School of Veterinary Medicine, Department of Veterinary Medicine
守屋大樹 モリヤ タイキ
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Website：Last Update :2026/09/03Researcher Profile and Settings
Name
MORIYA Taiki
Affiliation (Master)
School of Veterinary Medicine, Department of Veterinary Medicine
Affiliation & Job
Rakuno Gakuen University School of Veterinary Medicine Department of Veterinary Medicine Lecturer
Education
2011 04 - 2015 03 , Yamaguchi University, The United Graduate School of Veterinary Sciences 2013 05 - 2013 07 , Academy of Sciences of the Czech Republic, Institute of Experimental Medicine, Department of Molecular Neurophysiology 2005 04 - 2011 03 , Tottori University, Faculty of Agriculture, School of Veterinary Medicine Division of Basic Veterinary Sciences
Association Memberships
日本免疫学会 日本獣医学会
Research Activities
Research Areas
Life sciences, Neuroscience - general Life sciences, Laboratory animal science Life sciences, Veterinary medicine Life sciences, Animals: biochemistry, physiology, behavioral science Life sciences, Physiology Life sciences, Immunology
Research Interests
細胞移動, KikGR, バソプレシン, 腫瘍免疫, 樹状細胞,
Published Papers
CD4 + cell depletion accelerates dendritic cell migration and enhances resident dendritic cell proliferation in tumor-draining lymph nodes, Taiki Moriya; Mayuko Hashimoto; Mizuki Ueda; Tatsuya Aoyagi; Ayako Kawaguchi; Kentaro Takahashi; Kobayashi Tsukasa; Kazuki Doi; Hiroaki Hemmi; Tsuneyasu Kaisho; Satoshi Ueha; Koji Matsushima; Yutaka Kusumoto; Tatyana Chtanova; Michio Tomura, OncoImmunology, Informa UK Limited, 15, (1) , 2026 06 10 Sublingual immune cell clusters and dendritic cell distribution in the oral cavity., Yutaka Kusumoto; Mizuki Ueda; Mayuko Hashimoto; Haruka Takeuchi; Naoko Okada; Junya Yamamoto; Akiko Nishii; Atsuki Fujino; Akiho Kurahashi; Momoka Satoh; Yuki Iwasa; Koki Okamura; Karin Obazaki; Ryoto Kumagai; Naruya Sakamoto; Yuto Tanaka; Yukika Kamiya; Tetsushi Hoshida; Tsuneyasu Kaisho; Hiroaki Hemmi; Tomoya Katakai; Tetsuya Honda; Junichi Kikuta; Kosuke Kataoka; Ryoyo Ikebuchi; Taiki Moriya; Takahiro Adachi; Takeshi Watanabe; Masaru Ishii; Atsushi Miyawaki; Kenji Kabashima; Tatyana Chtanova; Michio Tomura, JCI insight, 2024 10 01 Near-infrared photoimmunotherapy induced tumor cell death enhances tumor dendritic cell migration., Taiki Moriya; Mayuko Hashimoto; Hina Matsushita; Shion Masuyama; Rina Yoshida; Ryuhei Okada; Aki Furusawa; Daiki Fujimura; Hiroaki Wakiyama; Takuya Kato; Peter L Choyke; Yutaka Kusumoto; Tatyana Chtanova; Hisataka Kobayashi; Michio Tomura, Cancer immunology, immunotherapy : CII, 71, (12) 3099 - 3106, 2022 05 28 The gut microbiota induces Peyer's-patch-dependent secretion of maternal IgA into milk., Katsuki Usami; Kanae Niimi; Ayumi Matsuo; Yoshihisa Suyama; Yoshifumi Sakai; Shintaro Sato; Kohtaro Fujihashi; Hiroshi Kiyono; Saeka Uchino; Mutsumi Furukawa; Jahidul Islam; Kaori Ito; Taiki Moriya; Yutaka Kusumoto; Michio Tomura; Russell C Hovey; Junichi Sugawara; Hiroshi Yoneyama; Haruki Kitazawa; Kouichi Watanabe; Hisashi Aso; Tomonori Nochi, Cell reports, 36, (10) 109655 - 109655, 2021 09 07 Cutting Edge: Recruitment, Retention, and Migration Underpin Functional Phenotypic Heterogeneity of Regulatory T Cells in Tumors., Ryoyo Ikebuchi; Taiki Moriya; Mizuki Ueda; Ippei Yasuda; Yutaka Kusumoto; Tatyana Chtanova; Michio Tomura, Journal of immunology (Baltimore, Md. : 1950), 207, (3) 771 - 776, 2021 08 01 Immunogenic tumor cell death promotes dendritic cell migration and inhibits tumor growth via enhanced T cell immunity., Taiki Moriya; Kurumi Kitagawa; Yuuki Hayakawa; Hiroaki Hemmi; Tsuneyasu Kaisho; Satoshi Ueha; Ryoyo Ikebuchi; Ippei Yasuda; Yasutaka Nakanishi; Tetsuya Honda; Koji Matsushima; Kenji Kabashima; Mizuki Ueda; Yutaka Kusumoto; Tatyana Chtanova; Michio Tomura, iScience, 24, (5) 102424 - 102424, 2021 05 21 Tracking the fate and migration of cells in live animals with cell-cycle indicators and photoconvertible proteins., Michio Tomura; Ryoyo Ikebuchi; Taiki Moriya; Yutaka Kusumoto, Journal of neuroscience methods, 355, 109127 - 109127, 2021 05 01 T cells are the main population in mouse breast milk and express similar profiles of tight junction proteins as those in mammary alveolar epithelial cells., Ryoyo Ikebuchi; Maika Fujimoto; Taiki Moriya; Yutaka Kusumoto; Ken Kobayashi; Michio Tomura, Journal of reproductive immunology, 140, 103137 - 103137, 2020 08 Prolyl-hydroxylase inhibitors reconstitute tumor blood vessels in mice., Shunji Nishide; Junji Uchida; Shinji Matsunaga; Kentaro Tokudome; Takehiro Yamaguchi; Kazuya Kabei; Taiki Moriya; Katsuyuki Miura; Tatsuya Nakatani; Shuhei Tomita, Journal of pharmacological sciences, 143, (2) 122 - 126, 2020 06 Dynamic Changes in the Phenotype of Dendritic Cells in the Uterus and Uterine Draining Lymph Nodes After Coitus., Ippei Yasuda; Tomoko Shima; Taiki Moriya; Ryoyo Ikebuchi; Yutaka Kusumoto; Akemi Ushijima; Akitoshi Nakashima; Michio Tomura; Shigeru Saito, Frontiers in immunology, 11, 557720 - 557720, 2020 Persistent Na+ influx drives L-type channel resting Ca2+ entry in rat melanotrophs., Tomohiko Kayano; Yuto Sasaki; Naoki Kitamura; Nobuya Harayama; Taiki Moriya; Govindan Dayanithi; Alexei Verkhratsky; Izumi Shibuya, Cell calcium, 79, 11 - 19, 2019 05 Functional Phenotypic Diversity of Regulatory T Cells Remaining in Inflamed Skin., Ryoyo Ikebuchi; Maika Fujimoto; Yasutaka Nakanishi; Hiromi Okuyama; Taiki Moriya; Yutaka Kusumoto; Michio Tomura, Frontiers in immunology, 10, 1098 - 1098, 2019 Regulatory T cells with superior immunosuppressive capacity emigrate from the inflamed colon to draining lymph nodes., Y Nakanishi; R Ikebuchi; T Chtanova; Y Kusumoto; H Okuyama; T Moriya; T Honda; K Kabashima; T Watanabe; Y Sakai; M Tomura, Mucosal immunology, 11, (2) 437 - 448, 2018 03 Epithelial membrane protein 3 (Emp3) downregulates induction and function of cytotoxic T lymphocytes by macrophages via TNF-α production., Yutaka Kusumoto; Hiromi Okuyama; Takuma Shibata; Kazunori Konno; Yusuke Takemoto; Daisuke Maekawa; Tomoyuki Kononaga; Takashi Ishii; Sachiko Akashi-Takamura; Shin-Ichiroh Saitoh; Ryoyo Ikebuchi; Taiki Moriya; Mizuki Ueda; Kensuke Miyake; Shiro Ono; Michio Tomura, Cellular immunology, 324, 33 - 41, 2018 02 マウスの母乳及び乳腺組織のリンパ球ポピュレーション解析, 池渕 良洋; 藤本 麻衣花; 中西 保貴; 奥山 洋美; 守屋 大樹; 楠本 豊; 戸村 道夫, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 159回, 433 - 433, 2016 08 Full-length transient receptor potential vanilloid 1 channels mediate calcium signals and possibly contribute to osmoreception in vasopressin neurones in the rat supraoptic nucleus., Taiki Moriya; Rina Shibasaki; Tomohiko Kayano; Nami Takebuchi; Momoko Ichimura; Naoki Kitamura; Atsushi Asano; Yoshinao Z Hosaka; Oksana Forostyak; Alexei Verkhratsky; Govindan Dayanithi; Izumi Shibuya, Cell calcium, 57, (1) 25 - 37, 2015 01 Analysis of G-protein-activated inward rectifying K(+) (GIRK) channel currents upon GABAB receptor activation in rat supraoptic neurons., Nobuya Harayama; Tomohiko Kayano; Taiki Moriya; Naoki Kitamura; Izumi Shibuya; Keiko Tanaka-Yamamoto; Yasuhito Uezono; Yoichi Ueta; Takeyoshi Sata, Brain research, 1591, 1 - 13, 2014 12 03 Chronic NGF treatment induces somatic hyperexcitability in cultured dorsal root ganglion neurons of the rat., Tomohiko Kayano; Naoki Kitamura; Taiki Moriya; Takeshi Kuwahara; You Komagiri; Emil C Toescu; Izumi Shibuya, Biomedical research (Tokyo, Japan), 34, (6) 329 - 42, 2013 Vasopressin-induced intracellular Ca²⁺ concentration responses in non-neuronal cells of the rat dorsal root ganglion., Taiki Moriya; Tomohiko Kayano; Naoki Kitamura; Yoshinao Z Hosaka; Atsushi Asano; Oksana Forostyak; Alexei Verkhratsky; Cedric Viero; Govindan Dayanithi; Emil C Toescu; Izumi Shibuya, Brain research, 1483, 1 - 12, 2012 11 05 Gymnopilin--a substance produced by the hallucinogenic mushroom, Gymnopilus junonius--mobilizes intracellular Ca(2+) in dorsal root ganglion cells., Shunsuke Miyazaki; Naoki Kitamura; Aiko Nishio; Saki Tanaka; Tomohiko Kayano; Taiki Moriya; Tsuyoshi Ichiyanagi; Norihiro Shimomura; Izumi Shibuya; Tadanori Aimi, Biomedical research (Tokyo, Japan), 33, (2) 111 - 8, 2012 04 Gymnopilin-a substance produced by the hallucinogenic mushroom, Gymnopilus junonius-mobilizes intracellular Ca2+ in dorsal root ganglion cells, Shunsuke Miyazaki; Naoki Kitamura; Aiko Nishio; Saki Tanaka; Tomohiko Kayano; Taiki Moriya; Tsuyoshi Ichiyanagi; Norihiro Shimomura; Izumi Shibuya; Tadanori Aimi, Biomedical Research, 33, (2) 111 - 118, 2012 Chronic treatment with NGF induces spontaneous fluctuations of intracellular Ca(2+) in icilin-sensitive dorsal root ganglion neurons of the rat., Tomohiko Kayano; Naoki Kitamura; Taiki Moriya; Atsushi Tsutsumi; Yui Ozaki; Govindan Dayanithi; Izumi Shibuya, The Journal of veterinary medical science, 72, (12) 1531 - 8, 2010 12
Books etc
ラボ必携 : フローサイトメトリーQ&A : 正しいデータを出すための100箇条, 戸村, 道夫, 羊土社, 2017 11 , 9784758122351
Conference Activities & Talks
メリチンと抗がん剤の併用による免疫原性細胞死誘導, 藤原 慶子; 戸村 道夫; 前田 尚之; 守屋 大樹, 第103回日本生理学会大会 Visualizing Tumor–Lymph Node Immune Cell Trafficking, 守屋大樹, チャレンジフィールド北海道シンポジウム メリチンを活用した抗腫瘍免疫増強の検討, 藤原慶子; 前田尚之; 戸村道夫; 守屋大樹, 第32回日本獣医がん学会 IgE抗体を介した抗腫瘍免疫応答の探索, 小河 千花; 守屋大樹; 寺岡 宏樹; 中村 達朗, 第7回 日本獣医薬理学・毒性学会・春季研究会 Immunogenic cell death promotes dendritic cell migration, Taiki Moriya, Chonnam National University - Rakuno Gakuen University Joint Symposium 2024 Near-infrared photoimmunotherapy induced tumor cell death enhances tumor-infiltrating dendritic cell migration via ATP-P2X7 receptor and Gαi protein-coupled receptor signaling pathways., The 52nd Annual Meeting of the Japanese Society for Immunology ATP-P2X7 receptor and HMGB1-TLR4 signaling pathways are involved in DT-induced enhancement of Ti-DC migration, Taiki Moriya; Yutaka Kusumoto; Michio Tomura, The 50th Annual Meeting of the Japanese Society for Immunology
Misc
Effector T cell migration from gut immune system, Mizuki Ueda; Taiki Moriya; Ryoyo Kusumoto; Yutaka Kusumoto; Michio Tomura, CYTOKINE, 100, 2017 12 Single-cell gene and protein expression analysis revealed functional and migratory heterogeneity in regulatory T cells of inflamed skin, Ryoyo Ikebuchi; Maika Fujimoto; Taiki Moriya; Hiromi Okuyama; Yutaka Kusumoto; Michio Tomura, CYTOKINE, 100, 2017 12 Immunization induces migration of MHC class II intermediate dendritic cells from immunized sites to draining lymph nodes, Taiki Moriya; Ryoyo Ikebuchi; Mizuki Ueda; Yutaka Kusumoto; Michio Tomura, CYTOKINE, 100, 2017 12 乳腺-初乳-新生仔と個体間を超えて移動するリンパ球に関する研究, 池渕良洋; 池渕良洋; 藤本麻衣花; 中西保貴; 奥山洋美; 守屋大樹; 楠本豊; 戸村道夫, 日本分子生物学会年会プログラム・要旨集(Web), 39th, 2016 ラット視索上核ニューロンに発現するTRPV1の機能解析および浸透圧受容器発現細胞の解析, 守屋大樹; 守屋大樹; 柴崎梨奈; 萱野智彦; 萱野智彦; 萱野智彦; 竹渕奈美; 市村桃子; 北村直樹; 北村直樹; 浅野淳; 浅野淳; 保坂善真; 保坂善真; DAYANITHI Govindan; 澁谷泉; 澁谷泉, 日本獣医学会学術集会講演要旨集, 157th, 2014 VASOPRESSIN-INDUCED [CA(2+)]I INCREASE IN NON-NEURONAL CELLS FROM RAT DORSAL ROOT GANGLIA, G. Dayanithi; T. Moriya; T. Kayano; N. Kitamura; Y. Hosaka; C. Viero; E. C. Toescu; O. Forostyak; Shibuya, I, GLIA, 59, 2011 10
Research Grants & Projects
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists, Elucidation of tumor infiltration molecular mechanism of dendritic cell progenitor cells and enhancement of antitumor immunity by application, 20220401, 20250331, 守屋 大樹 Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory), Elucidation of in vivo kinetics of leukemia cell differentiation and proliferation in blast crisis, 20200730, 20220331, 戸村 道夫; 守屋 大樹; 楠本 豊; 岩崎 正幸, 当研究は、急性転化後の白血病幹細胞および白血病細胞の全身レベルでの分化・動態・細胞周期情報を取得し、取得した情報をもとに細胞を分画し、分画間の分子発現情報比較から急性転化後の白血病幹細胞の性状を明らかにし、さらに新規の治療標的分子を見出すことを目的としている。
令和2年度は、急性転化後の白血病幹細胞および白血病細胞の全身レベルでの動態・細胞周期情報を取得できる急性転化モデルマウスを作成し、白血病細胞の細胞動態と細胞周期解析を開始した。生体内細胞動態を解析できるKikGRマウス、細胞周期を可視化できるFucci-Tgマウス、およびKikGR/Fucciマウスの骨髄c-kit+細胞に、ヒトCML原因遺伝子のBCR-ABL(BA)とAMLへの急性転化に関わるNUP98-HOXA9(NH)の両遺伝子、あるいはNHを単独で導入し不死化c-kit+細胞を得た。これら細胞を放射線照射したマウスに移入後、末梢血中の白血病細胞の頻度をモニターし、発症は骨髄細胞のスメア標本で確認した。発症確認後、採取した骨髄細胞(一次骨髄)を放射線照射したマウスに移入し白血病の発症を確認して骨髄(二次骨髄)を取得した。 現在までに、KikGRおよびFucci発現のBA/NH遺伝子導入の急性転化、ならびにNH単独発現の慢性白血病モデルを作成した。
発症した白血病マウスの細胞を抗体染色し、報告されているマーカーによる幹細胞含有画分と蛍光シグナルを同時検出できるフローサイトメトリー染色パネルを作成し、解析を開始した。KikGR発現白血病モデルマウスの骨髄に光照射し、白血病細胞のマーキングと他臓器への移動を追跡できる評価系を確立した。
白血病幹細胞の長期滞在ニッチの可視化検出を試みるために、長期接触細胞をマークできる分割GFPのGRAPHICシステムのアクセプターGFPマウスと、ドナーGFPマウスを導入した。 Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A), Elucidation of reproductive cycle-specific immunological and microbiological dynamics using bioimaging, 20180401, 20220331, Nochi Tomonori, The evolutionary strategy of transferring maternal antibodies via milk profoundly impacts the survival, lifelong health and wellbeing of all neonates. While there has been increased recognition that interorgan connectivity influences the quality of a mother’s milk, potentially to personalize it for her offspring, the underlying bases for these processes are incompletely resolved. Here we define an essential role of Peyer’s patches (PP) for the generation of plasma cells that secrete maternal IgA into milk. Our metagenomic analysis reveals that the presence of certain residential microorganisms in the gastrointestinal tract, such as Bacteroides acidifaciens and Prevotella buccalis, is indispensable for the programming of maternal IgA synthesis prior to lactational transfer. Our data provide important insights into how the microbiome of the maternal gastrointestinal environment, specifically through the PP, can be communicated to the next generation via milk. Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Role of Immune Cell Clusters in Sublingual Mucosal Tissue in Oral Immune Responses, 20180401, 20210331, Kusumoto Yutaka, To clarify the mechanisms of immune responses in the oral mucosa, we analyzed the properties, constituent cells, and functional roles of immune cell clusters in the sublingual mucosa. The clusters in steady state contained regulatory T cells (Treg), unlike other tissues. The subsets of constituent T cells significantly changed depending on the immune responses. The coexistence of a subsets of dendritic cells, which were able to activate these T cells, suggested that the clusters had a local immunomodulatory mechanism. These findings may lead to the elucidation of the mechanisms of sublingually administered vaccines and sublingual immunotherapy. 日本学術振興会, 科学研究費助成事業 若手研究, CD103+樹状細胞の腫瘍局在促進による抗腫瘍免疫増強, 20180401, 20210331, 守屋 大樹, 腫瘍浸潤CD103+樹状細胞の中には、リンパ節に移行し、抗原提示により細胞傷害性T 細胞(CTL)を誘導する細胞に加え、腫瘍に留まりCTLに抗原提示をする細胞も存在することが示唆されている。 本研究は(1)腫瘍に「留まる」細胞が利用する分子メカニズムを解明し、(2)留まる分子を利用したCD103+樹状細胞の腫瘍局在促進により抗腫瘍CTLへの抗原提示促進を試みる。2018年度は(1)に関して、腫瘍に「留まる」CD103+樹状細胞の単細胞遺伝子発現解析のための準備を、(2)に関して、抗腫瘍CTLへの抗原提示の検出方法の確立を行った。
（１）腫瘍に「留まる」CD103+樹状細胞が利用する分子メカニズム解明のため申請者の所属機関外にて網羅的遺伝子発現解析を行う予定であったが、申請者の所属機関に新たにシングルセル解析システム（BD Rhapsody）の導入が決定し所属機関での実施が可能となった。このためBD Rhapsodyでの解析のための細胞分画の決定等の条件設定を行った。
（２）S/G2/M期の細胞がmAzami-Green(mAG), G1期の細胞がmKusabira-Orange2蛍光タンパク質を発現し細胞周期を可視化解析可能なFucciマウスを用い、本研究で用いている腫瘍のモデル抗原である卵白アルブミン(OVA)に対する抗原特異的CD8+T細胞をOVAテトラマー陽性細胞にて同定、フローサイトメトリー法により解析を行った。すると腫瘍組織内OVA抗原特異的CD8+T細胞の一部においてmAG+の増殖期の細胞を検出することができた。この方法を用い、CD103+樹状細胞の腫瘍局在増強時の抗原提示増強の有無について定量解析を行う予定である。 Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Elucidation of mechanisms underlying multi-organ cooperative regulation by analyzing immune cells migrated from gut to whole body and their functional molecules, 20160401, 20190331, Michio Tomura; KUSUMOTO yutaka; MORIYA taiki; ADACHI takahiro; Ueda mizuki; NISHII akiko, The intestinal environment is thought to be involved in systemic diseases such as cancer, obesity and diabetes, however the mechanism is poorly understood. Therefore, in this study, we aimed to clarify the mechanism of multi-organ cooperation starting from the intestinal tract, focusing on immune cells migrated from intestinal tissue to multiple organs. We analyzed cells migrated from gut-related tissues to whole body by KikGR mice that can track cell movement between organs in normal food intake, high fat diet intake mice, and enteric commensal bacteria-free mice with antibiotics. Then, we clarified immune cell subsets and phenotypes that are migrated to the immune system. Furthermore, we found the mechanism that enteric tissue-derived cells stimulated by commensal bacteria migrate to the bone marrow and express function. Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Establishment of skin inflammation drug to accumulate high-functional regulatory T cells by single-cell gene expression analysis, 20150401, 20170331, Ikebuchi Ryoyo; TOMURA Michio; KUSUMOTO Yutaka; MORIYA Taiki; TERAGUCHI Shunsuke; Alexis Vandenbon; NAKAYAMA Takashi; MATSUO Kazuhiko, We identified several regulatory T cell (Treg) subsets in inflamed skin tissue of mouse line expressing photoconvertible protein KikGR, by which we can distinguish skin-coming and -remaining lymphocytes, by multi-parameter single-cell gene and protein expression analysis. Each subset expressed each set of functional and migration-related molecules and showed different capabilities to remain in inflamed skin. We distinguished skin-coming and -remaining Tregs without KikGR expression data by multi-parameter single-cell expression analysis of functional and migration-related molecules. We also tried to identify master molecules of Treg subset differentiation and establish method to collect the Treg subsets. This research suggests that Tregs expressing different functional molecules showed different migration status in inflamed tissues. 日本学術振興会, 科学研究費助成事業 特別研究員奨励費, ラットDRGにおけるAVPを介したニューロンーグリア細胞間の相互作用の解明, 2012, 2014, 守屋 大樹, 昨年度までにラットSONニューロンに発現する浸透圧受容器のうち少なくとも一部は全長TRPV1によって構成されていることを明らかにした。しかし、浸透圧受容器と報告されているホモ4量体のTRPV1では逆転電位の値などいくつか性質が異なることが分かっている。
今年度に実施した実験の中でラットSONでエクソンの1-5番目を欠く新しいTRPV1スプライスバリアントを発見し、この分子をTRPV1_SONと名付けた。
TRPV1は4量体で一つのチャネルを形成し、他の分子とヘテロ4量体を形成しうること、そしてヘテロ4量体分子はCapsaicinに対する応答が減弱する等ホモ4量体TRPV1が示す性質が変化することが報告されている。
我々はラットSONに発現する浸透圧受容器は全長TRPV1とTRPV1_SONのヘテロ4量体で構成されているという仮説を立てた。これを検証するために全長TRPV1、TRPV1_SON、TRPV1とTRPV1_SONを接続した遺伝子をそれぞれ組み込んだベクターを作成し、それぞれのベクターを導入した細胞の浸透圧応答を調べることを検討している。
これまで全長TRPV1、TRPV1_SONベクターを作成した。全長TRPV1発現細胞にCapsaicinを適用するとほぼ全ての細胞で応答が見られた。応答が見られた細胞のうち約20%の細胞は高浸透圧刺激にも応答したが、繰り返しの高浸透圧刺激には応答しなかった。
TRPV1_SON発現細胞ではcapsaicinおよび高浸透圧刺激に応答しなかった。
以上の結果からTRPV1のみ、TRPV1_SONのみを強制発現した細胞ではいずれもラットSONニューロンで見られた性質を再現することが出来なかった。このため、ラットSONニューロンに発現する浸透圧受容器はそれぞれの分子のみで構成されている分子ではないことが考えられた。
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Last Update :2026/09/03
Researcher Profile and Settings
Name
| MORIYA Taiki |
Affiliation (Master)
| School of Veterinary Medicine, Department of Veterinary Medicine |
Affiliation & Job
| Rakuno Gakuen University |
| School of Veterinary Medicine Department of Veterinary Medicine |
| Lecturer |
Education
| 2011 04 - 2015 03 , Yamaguchi University, The United Graduate School of Veterinary Sciences |
| 2013 05 - 2013 07 , Academy of Sciences of the Czech Republic, Institute of Experimental Medicine, Department of Molecular Neurophysiology |
| 2005 04 - 2011 03 , Tottori University, Faculty of Agriculture, School of Veterinary Medicine Division of Basic Veterinary Sciences |
Association Memberships
| 日本免疫学会 |
| 日本獣医学会 |
Research Activities
Research Areas
| Life sciences, Neuroscience - general |
| Life sciences, Laboratory animal science |
| Life sciences, Veterinary medicine |
| Life sciences, Animals: biochemistry, physiology, behavioral science |
| Life sciences, Physiology |
| Life sciences, Immunology |
Research Interests
| 細胞移動, |
| KikGR, |
| バソプレシン, |
| 腫瘍免疫, |
| 樹状細胞, |
Published Papers
| CD4 + cell depletion accelerates dendritic cell migration and enhances resident dendritic cell proliferation in tumor-draining lymph nodes, Taiki Moriya; Mayuko Hashimoto; Mizuki Ueda; Tatsuya Aoyagi; Ayako Kawaguchi; Kentaro Takahashi; Kobayashi Tsukasa; Kazuki Doi; Hiroaki Hemmi; Tsuneyasu Kaisho; Satoshi Ueha; Koji Matsushima; Yutaka Kusumoto; Tatyana Chtanova; Michio Tomura, OncoImmunology, Informa UK Limited, 15, (1) , 2026 06 10 |
| Sublingual immune cell clusters and dendritic cell distribution in the oral cavity., Yutaka Kusumoto; Mizuki Ueda; Mayuko Hashimoto; Haruka Takeuchi; Naoko Okada; Junya Yamamoto; Akiko Nishii; Atsuki Fujino; Akiho Kurahashi; Momoka Satoh; Yuki Iwasa; Koki Okamura; Karin Obazaki; Ryoto Kumagai; Naruya Sakamoto; Yuto Tanaka; Yukika Kamiya; Tetsushi Hoshida; Tsuneyasu Kaisho; Hiroaki Hemmi; Tomoya Katakai; Tetsuya Honda; Junichi Kikuta; Kosuke Kataoka; Ryoyo Ikebuchi; Taiki Moriya; Takahiro Adachi; Takeshi Watanabe; Masaru Ishii; Atsushi Miyawaki; Kenji Kabashima; Tatyana Chtanova; Michio Tomura, JCI insight, 2024 10 01 |
| Near-infrared photoimmunotherapy induced tumor cell death enhances tumor dendritic cell migration., Taiki Moriya; Mayuko Hashimoto; Hina Matsushita; Shion Masuyama; Rina Yoshida; Ryuhei Okada; Aki Furusawa; Daiki Fujimura; Hiroaki Wakiyama; Takuya Kato; Peter L Choyke; Yutaka Kusumoto; Tatyana Chtanova; Hisataka Kobayashi; Michio Tomura, Cancer immunology, immunotherapy : CII, 71, (12) 3099 - 3106, 2022 05 28 |
| The gut microbiota induces Peyer's-patch-dependent secretion of maternal IgA into milk., Katsuki Usami; Kanae Niimi; Ayumi Matsuo; Yoshihisa Suyama; Yoshifumi Sakai; Shintaro Sato; Kohtaro Fujihashi; Hiroshi Kiyono; Saeka Uchino; Mutsumi Furukawa; Jahidul Islam; Kaori Ito; Taiki Moriya; Yutaka Kusumoto; Michio Tomura; Russell C Hovey; Junichi Sugawara; Hiroshi Yoneyama; Haruki Kitazawa; Kouichi Watanabe; Hisashi Aso; Tomonori Nochi, Cell reports, 36, (10) 109655 - 109655, 2021 09 07 |
| Cutting Edge: Recruitment, Retention, and Migration Underpin Functional Phenotypic Heterogeneity of Regulatory T Cells in Tumors., Ryoyo Ikebuchi; Taiki Moriya; Mizuki Ueda; Ippei Yasuda; Yutaka Kusumoto; Tatyana Chtanova; Michio Tomura, Journal of immunology (Baltimore, Md. : 1950), 207, (3) 771 - 776, 2021 08 01 |
| Immunogenic tumor cell death promotes dendritic cell migration and inhibits tumor growth via enhanced T cell immunity., Taiki Moriya; Kurumi Kitagawa; Yuuki Hayakawa; Hiroaki Hemmi; Tsuneyasu Kaisho; Satoshi Ueha; Ryoyo Ikebuchi; Ippei Yasuda; Yasutaka Nakanishi; Tetsuya Honda; Koji Matsushima; Kenji Kabashima; Mizuki Ueda; Yutaka Kusumoto; Tatyana Chtanova; Michio Tomura, iScience, 24, (5) 102424 - 102424, 2021 05 21 |
| Tracking the fate and migration of cells in live animals with cell-cycle indicators and photoconvertible proteins., Michio Tomura; Ryoyo Ikebuchi; Taiki Moriya; Yutaka Kusumoto, Journal of neuroscience methods, 355, 109127 - 109127, 2021 05 01 |
| T cells are the main population in mouse breast milk and express similar profiles of tight junction proteins as those in mammary alveolar epithelial cells., Ryoyo Ikebuchi; Maika Fujimoto; Taiki Moriya; Yutaka Kusumoto; Ken Kobayashi; Michio Tomura, Journal of reproductive immunology, 140, 103137 - 103137, 2020 08 |
| Prolyl-hydroxylase inhibitors reconstitute tumor blood vessels in mice., Shunji Nishide; Junji Uchida; Shinji Matsunaga; Kentaro Tokudome; Takehiro Yamaguchi; Kazuya Kabei; Taiki Moriya; Katsuyuki Miura; Tatsuya Nakatani; Shuhei Tomita, Journal of pharmacological sciences, 143, (2) 122 - 126, 2020 06 |
| Dynamic Changes in the Phenotype of Dendritic Cells in the Uterus and Uterine Draining Lymph Nodes After Coitus., Ippei Yasuda; Tomoko Shima; Taiki Moriya; Ryoyo Ikebuchi; Yutaka Kusumoto; Akemi Ushijima; Akitoshi Nakashima; Michio Tomura; Shigeru Saito, Frontiers in immunology, 11, 557720 - 557720, 2020 |
| Persistent Na+ influx drives L-type channel resting Ca2+ entry in rat melanotrophs., Tomohiko Kayano; Yuto Sasaki; Naoki Kitamura; Nobuya Harayama; Taiki Moriya; Govindan Dayanithi; Alexei Verkhratsky; Izumi Shibuya, Cell calcium, 79, 11 - 19, 2019 05 |
| Functional Phenotypic Diversity of Regulatory T Cells Remaining in Inflamed Skin., Ryoyo Ikebuchi; Maika Fujimoto; Yasutaka Nakanishi; Hiromi Okuyama; Taiki Moriya; Yutaka Kusumoto; Michio Tomura, Frontiers in immunology, 10, 1098 - 1098, 2019 |
| Regulatory T cells with superior immunosuppressive capacity emigrate from the inflamed colon to draining lymph nodes., Y Nakanishi; R Ikebuchi; T Chtanova; Y Kusumoto; H Okuyama; T Moriya; T Honda; K Kabashima; T Watanabe; Y Sakai; M Tomura, Mucosal immunology, 11, (2) 437 - 448, 2018 03 |
| Epithelial membrane protein 3 (Emp3) downregulates induction and function of cytotoxic T lymphocytes by macrophages via TNF-α production., Yutaka Kusumoto; Hiromi Okuyama; Takuma Shibata; Kazunori Konno; Yusuke Takemoto; Daisuke Maekawa; Tomoyuki Kononaga; Takashi Ishii; Sachiko Akashi-Takamura; Shin-Ichiroh Saitoh; Ryoyo Ikebuchi; Taiki Moriya; Mizuki Ueda; Kensuke Miyake; Shiro Ono; Michio Tomura, Cellular immunology, 324, 33 - 41, 2018 02 |
| マウスの母乳及び乳腺組織のリンパ球ポピュレーション解析, 池渕 良洋; 藤本 麻衣花; 中西 保貴; 奥山 洋美; 守屋 大樹; 楠本 豊; 戸村 道夫, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 159回, 433 - 433, 2016 08 |
| Full-length transient receptor potential vanilloid 1 channels mediate calcium signals and possibly contribute to osmoreception in vasopressin neurones in the rat supraoptic nucleus., Taiki Moriya; Rina Shibasaki; Tomohiko Kayano; Nami Takebuchi; Momoko Ichimura; Naoki Kitamura; Atsushi Asano; Yoshinao Z Hosaka; Oksana Forostyak; Alexei Verkhratsky; Govindan Dayanithi; Izumi Shibuya, Cell calcium, 57, (1) 25 - 37, 2015 01 |
| Analysis of G-protein-activated inward rectifying K(+) (GIRK) channel currents upon GABAB receptor activation in rat supraoptic neurons., Nobuya Harayama; Tomohiko Kayano; Taiki Moriya; Naoki Kitamura; Izumi Shibuya; Keiko Tanaka-Yamamoto; Yasuhito Uezono; Yoichi Ueta; Takeyoshi Sata, Brain research, 1591, 1 - 13, 2014 12 03 |
| Chronic NGF treatment induces somatic hyperexcitability in cultured dorsal root ganglion neurons of the rat., Tomohiko Kayano; Naoki Kitamura; Taiki Moriya; Takeshi Kuwahara; You Komagiri; Emil C Toescu; Izumi Shibuya, Biomedical research (Tokyo, Japan), 34, (6) 329 - 42, 2013 |
| Vasopressin-induced intracellular Ca²⁺ concentration responses in non-neuronal cells of the rat dorsal root ganglion., Taiki Moriya; Tomohiko Kayano; Naoki Kitamura; Yoshinao Z Hosaka; Atsushi Asano; Oksana Forostyak; Alexei Verkhratsky; Cedric Viero; Govindan Dayanithi; Emil C Toescu; Izumi Shibuya, Brain research, 1483, 1 - 12, 2012 11 05 |
| Gymnopilin--a substance produced by the hallucinogenic mushroom, Gymnopilus junonius--mobilizes intracellular Ca(2+) in dorsal root ganglion cells., Shunsuke Miyazaki; Naoki Kitamura; Aiko Nishio; Saki Tanaka; Tomohiko Kayano; Taiki Moriya; Tsuyoshi Ichiyanagi; Norihiro Shimomura; Izumi Shibuya; Tadanori Aimi, Biomedical research (Tokyo, Japan), 33, (2) 111 - 8, 2012 04 |
| Gymnopilin-a substance produced by the hallucinogenic mushroom, Gymnopilus junonius-mobilizes intracellular Ca2+ in dorsal root ganglion cells, Shunsuke Miyazaki; Naoki Kitamura; Aiko Nishio; Saki Tanaka; Tomohiko Kayano; Taiki Moriya; Tsuyoshi Ichiyanagi; Norihiro Shimomura; Izumi Shibuya; Tadanori Aimi, Biomedical Research, 33, (2) 111 - 118, 2012 |
| Chronic treatment with NGF induces spontaneous fluctuations of intracellular Ca(2+) in icilin-sensitive dorsal root ganglion neurons of the rat., Tomohiko Kayano; Naoki Kitamura; Taiki Moriya; Atsushi Tsutsumi; Yui Ozaki; Govindan Dayanithi; Izumi Shibuya, The Journal of veterinary medical science, 72, (12) 1531 - 8, 2010 12 |
Books etc
| ラボ必携 : フローサイトメトリーQ&A : 正しいデータを出すための100箇条, 戸村, 道夫, 羊土社, 2017 11 , 9784758122351 |
Conference Activities & Talks
| メリチンと抗がん剤の併用による免疫原性細胞死誘導, 藤原 慶子; 戸村 道夫; 前田 尚之; 守屋 大樹, 第103回日本生理学会大会 |
| Visualizing Tumor–Lymph Node Immune Cell Trafficking, 守屋大樹, チャレンジフィールド北海道シンポジウム |
| メリチンを活用した抗腫瘍免疫増強の検討, 藤原慶子; 前田尚之; 戸村道夫; 守屋大樹, 第32回日本獣医がん学会 |
| IgE抗体を介した抗腫瘍免疫応答の探索, 小河 千花; 守屋大樹; 寺岡 宏樹; 中村 達朗, 第7回 日本獣医薬理学・毒性学会・春季研究会 |
| Immunogenic cell death promotes dendritic cell migration, Taiki Moriya, Chonnam National University - Rakuno Gakuen University Joint Symposium 2024 |
| Near-infrared photoimmunotherapy induced tumor cell death enhances tumor-infiltrating dendritic cell migration via ATP-P2X7 receptor and Gαi protein-coupled receptor signaling pathways., The 52nd Annual Meeting of the Japanese Society for Immunology |
| ATP-P2X7 receptor and HMGB1-TLR4 signaling pathways are involved in DT-induced enhancement of Ti-DC migration, Taiki Moriya; Yutaka Kusumoto; Michio Tomura, The 50th Annual Meeting of the Japanese Society for Immunology |
Misc
| Effector T cell migration from gut immune system, Mizuki Ueda; Taiki Moriya; Ryoyo Kusumoto; Yutaka Kusumoto; Michio Tomura, CYTOKINE, 100, 2017 12 |
| Single-cell gene and protein expression analysis revealed functional and migratory heterogeneity in regulatory T cells of inflamed skin, Ryoyo Ikebuchi; Maika Fujimoto; Taiki Moriya; Hiromi Okuyama; Yutaka Kusumoto; Michio Tomura, CYTOKINE, 100, 2017 12 |
| Immunization induces migration of MHC class II intermediate dendritic cells from immunized sites to draining lymph nodes, Taiki Moriya; Ryoyo Ikebuchi; Mizuki Ueda; Yutaka Kusumoto; Michio Tomura, CYTOKINE, 100, 2017 12 |
| 乳腺-初乳-新生仔と個体間を超えて移動するリンパ球に関する研究, 池渕良洋; 池渕良洋; 藤本麻衣花; 中西保貴; 奥山洋美; 守屋大樹; 楠本豊; 戸村道夫, 日本分子生物学会年会プログラム・要旨集(Web), 39th, 2016 |
| ラット視索上核ニューロンに発現するTRPV1の機能解析および浸透圧受容器発現細胞の解析, 守屋大樹; 守屋大樹; 柴崎梨奈; 萱野智彦; 萱野智彦; 萱野智彦; 竹渕奈美; 市村桃子; 北村直樹; 北村直樹; 浅野淳; 浅野淳; 保坂善真; 保坂善真; DAYANITHI Govindan; 澁谷泉; 澁谷泉, 日本獣医学会学術集会講演要旨集, 157th, 2014 |
| VASOPRESSIN-INDUCED [CA(2+)]I INCREASE IN NON-NEURONAL CELLS FROM RAT DORSAL ROOT GANGLIA, G. Dayanithi; T. Moriya; T. Kayano; N. Kitamura; Y. Hosaka; C. Viero; E. C. Toescu; O. Forostyak; Shibuya, I, GLIA, 59, 2011 10 |
Research Grants & Projects
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists, Elucidation of tumor infiltration molecular mechanism of dendritic cell progenitor cells and enhancement of antitumor immunity by application, 20220401, 20250331, 守屋 大樹 |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory), Elucidation of in vivo kinetics of leukemia cell differentiation and proliferation in blast crisis, 20200730, 20220331, 戸村 道夫; 守屋 大樹; 楠本 豊; 岩崎 正幸, 当研究は、急性転化後の白血病幹細胞および白血病細胞の全身レベルでの分化・動態・細胞周期情報を取得し、取得した情報をもとに細胞を分画し、分画間の分子発現情報比較から急性転化後の白血病幹細胞の性状を明らかにし、さらに新規の治療標的分子を見出すことを目的としている。 令和2年度は、急性転化後の白血病幹細胞および白血病細胞の全身レベルでの動態・細胞周期情報を取得できる急性転化モデルマウスを作成し、白血病細胞の細胞動態と細胞周期解析を開始した。生体内細胞動態を解析できるKikGRマウス、細胞周期を可視化できるFucci-Tgマウス、およびKikGR/Fucciマウスの骨髄c-kit+細胞に、ヒトCML原因遺伝子のBCR-ABL(BA)とAMLへの急性転化に関わるNUP98-HOXA9(NH)の両遺伝子、あるいはNHを単独で導入し不死化c-kit+細胞を得た。これら細胞を放射線照射したマウスに移入後、末梢血中の白血病細胞の頻度をモニターし、発症は骨髄細胞のスメア標本で確認した。発症確認後、採取した骨髄細胞(一次骨髄)を放射線照射したマウスに移入し白血病の発症を確認して骨髄(二次骨髄)を取得した。 現在までに、KikGRおよびFucci発現のBA/NH遺伝子導入の急性転化、ならびにNH単独発現の慢性白血病モデルを作成した。 発症した白血病マウスの細胞を抗体染色し、報告されているマーカーによる幹細胞含有画分と蛍光シグナルを同時検出できるフローサイトメトリー染色パネルを作成し、解析を開始した。KikGR発現白血病モデルマウスの骨髄に光照射し、白血病細胞のマーキングと他臓器への移動を追跡できる評価系を確立した。 白血病幹細胞の長期滞在ニッチの可視化検出を試みるために、長期接触細胞をマークできる分割GFPのGRAPHICシステムのアクセプターGFPマウスと、ドナーGFPマウスを導入した。 |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A), Elucidation of reproductive cycle-specific immunological and microbiological dynamics using bioimaging, 20180401, 20220331, Nochi Tomonori, The evolutionary strategy of transferring maternal antibodies via milk profoundly impacts the survival, lifelong health and wellbeing of all neonates. While there has been increased recognition that interorgan connectivity influences the quality of a mother’s milk, potentially to personalize it for her offspring, the underlying bases for these processes are incompletely resolved. Here we define an essential role of Peyer’s patches (PP) for the generation of plasma cells that secrete maternal IgA into milk. Our metagenomic analysis reveals that the presence of certain residential microorganisms in the gastrointestinal tract, such as Bacteroides acidifaciens and Prevotella buccalis, is indispensable for the programming of maternal IgA synthesis prior to lactational transfer. Our data provide important insights into how the microbiome of the maternal gastrointestinal environment, specifically through the PP, can be communicated to the next generation via milk. |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Role of Immune Cell Clusters in Sublingual Mucosal Tissue in Oral Immune Responses, 20180401, 20210331, Kusumoto Yutaka, To clarify the mechanisms of immune responses in the oral mucosa, we analyzed the properties, constituent cells, and functional roles of immune cell clusters in the sublingual mucosa. The clusters in steady state contained regulatory T cells (Treg), unlike other tissues. The subsets of constituent T cells significantly changed depending on the immune responses. The coexistence of a subsets of dendritic cells, which were able to activate these T cells, suggested that the clusters had a local immunomodulatory mechanism. These findings may lead to the elucidation of the mechanisms of sublingually administered vaccines and sublingual immunotherapy. |
| 日本学術振興会, 科学研究費助成事業 若手研究, CD103+樹状細胞の腫瘍局在促進による抗腫瘍免疫増強, 20180401, 20210331, 守屋 大樹, 腫瘍浸潤CD103+樹状細胞の中には、リンパ節に移行し、抗原提示により細胞傷害性T 細胞(CTL)を誘導する細胞に加え、腫瘍に留まりCTLに抗原提示をする細胞も存在することが示唆されている。 本研究は(1)腫瘍に「留まる」細胞が利用する分子メカニズムを解明し、(2)留まる分子を利用したCD103+樹状細胞の腫瘍局在促進により抗腫瘍CTLへの抗原提示促進を試みる。2018年度は(1)に関して、腫瘍に「留まる」CD103+樹状細胞の単細胞遺伝子発現解析のための準備を、(2)に関して、抗腫瘍CTLへの抗原提示の検出方法の確立を行った。 （１）腫瘍に「留まる」CD103+樹状細胞が利用する分子メカニズム解明のため申請者の所属機関外にて網羅的遺伝子発現解析を行う予定であったが、申請者の所属機関に新たにシングルセル解析システム（BD Rhapsody）の導入が決定し所属機関での実施が可能となった。このためBD Rhapsodyでの解析のための細胞分画の決定等の条件設定を行った。 （２）S/G2/M期の細胞がmAzami-Green(mAG), G1期の細胞がmKusabira-Orange2蛍光タンパク質を発現し細胞周期を可視化解析可能なFucciマウスを用い、本研究で用いている腫瘍のモデル抗原である卵白アルブミン(OVA)に対する抗原特異的CD8+T細胞をOVAテトラマー陽性細胞にて同定、フローサイトメトリー法により解析を行った。すると腫瘍組織内OVA抗原特異的CD8+T細胞の一部においてmAG+の増殖期の細胞を検出することができた。この方法を用い、CD103+樹状細胞の腫瘍局在増強時の抗原提示増強の有無について定量解析を行う予定である。 |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Elucidation of mechanisms underlying multi-organ cooperative regulation by analyzing immune cells migrated from gut to whole body and their functional molecules, 20160401, 20190331, Michio Tomura; KUSUMOTO yutaka; MORIYA taiki; ADACHI takahiro; Ueda mizuki; NISHII akiko, The intestinal environment is thought to be involved in systemic diseases such as cancer, obesity and diabetes, however the mechanism is poorly understood. Therefore, in this study, we aimed to clarify the mechanism of multi-organ cooperation starting from the intestinal tract, focusing on immune cells migrated from intestinal tissue to multiple organs. We analyzed cells migrated from gut-related tissues to whole body by KikGR mice that can track cell movement between organs in normal food intake, high fat diet intake mice, and enteric commensal bacteria-free mice with antibiotics. Then, we clarified immune cell subsets and phenotypes that are migrated to the immune system. Furthermore, we found the mechanism that enteric tissue-derived cells stimulated by commensal bacteria migrate to the bone marrow and express function. |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Establishment of skin inflammation drug to accumulate high-functional regulatory T cells by single-cell gene expression analysis, 20150401, 20170331, Ikebuchi Ryoyo; TOMURA Michio; KUSUMOTO Yutaka; MORIYA Taiki; TERAGUCHI Shunsuke; Alexis Vandenbon; NAKAYAMA Takashi; MATSUO Kazuhiko, We identified several regulatory T cell (Treg) subsets in inflamed skin tissue of mouse line expressing photoconvertible protein KikGR, by which we can distinguish skin-coming and -remaining lymphocytes, by multi-parameter single-cell gene and protein expression analysis. Each subset expressed each set of functional and migration-related molecules and showed different capabilities to remain in inflamed skin. We distinguished skin-coming and -remaining Tregs without KikGR expression data by multi-parameter single-cell expression analysis of functional and migration-related molecules. We also tried to identify master molecules of Treg subset differentiation and establish method to collect the Treg subsets. This research suggests that Tregs expressing different functional molecules showed different migration status in inflamed tissues. |
| 日本学術振興会, 科学研究費助成事業 特別研究員奨励費, ラットDRGにおけるAVPを介したニューロンーグリア細胞間の相互作用の解明, 2012, 2014, 守屋 大樹, 昨年度までにラットSONニューロンに発現する浸透圧受容器のうち少なくとも一部は全長TRPV1によって構成されていることを明らかにした。しかし、浸透圧受容器と報告されているホモ4量体のTRPV1では逆転電位の値などいくつか性質が異なることが分かっている。 今年度に実施した実験の中でラットSONでエクソンの1-5番目を欠く新しいTRPV1スプライスバリアントを発見し、この分子をTRPV1_SONと名付けた。 TRPV1は4量体で一つのチャネルを形成し、他の分子とヘテロ4量体を形成しうること、そしてヘテロ4量体分子はCapsaicinに対する応答が減弱する等ホモ4量体TRPV1が示す性質が変化することが報告されている。 我々はラットSONに発現する浸透圧受容器は全長TRPV1とTRPV1_SONのヘテロ4量体で構成されているという仮説を立てた。これを検証するために全長TRPV1、TRPV1_SON、TRPV1とTRPV1_SONを接続した遺伝子をそれぞれ組み込んだベクターを作成し、それぞれのベクターを導入した細胞の浸透圧応答を調べることを検討している。 これまで全長TRPV1、TRPV1_SONベクターを作成した。全長TRPV1発現細胞にCapsaicinを適用するとほぼ全ての細胞で応答が見られた。応答が見られた細胞のうち約20%の細胞は高浸透圧刺激にも応答したが、繰り返しの高浸透圧刺激には応答しなかった。 TRPV1_SON発現細胞ではcapsaicinおよび高浸透圧刺激に応答しなかった。 以上の結果からTRPV1のみ、TRPV1_SONのみを強制発現した細胞ではいずれもラットSONニューロンで見られた性質を再現することが出来なかった。このため、ラットSONニューロンに発現する浸透圧受容器はそれぞれの分子のみで構成されている分子ではないことが考えられた。 |
教員総覧