School of Veterinary Medicine
植田弘美 ウエダ ヒロミ
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Website：Last Update :2024/12/18Researcher Profile and Settings
Name
UEDA Hiromi
Affiliation (Master)
School of Veterinary Medicine
Affiliation & Job
Rakuno Gakuen University School of Veterinary Medicine, Department of Veterinary Medicine
Degree
Veterinary Medical Doctor, Rakuno Gakuen University Veterinary Medical Master, Rakuno Gakuen University
Association Memberships
JAPAN VETERINARY MEDICAL ASSOCIATION THE JAPANESE SOCIETY OF MICROSCOPY 日本組織細胞化学会 日本解剖学会 日本獣医学会
Research Activities
Research Areas
Life sciences, Anatomy Life sciences, Veterinary medicine
Research Interests
herbal medicine, fatty liver, liver, diabetes, histology,
Published Papers
Morphological and biochemical characterization of Holstein cow skin at the tail root region susceptible to <i>Chorioptes bovis</i> and <i>texanus</i> parasitism, Emi FUJII; Hotaka HASHIMOTO; Shin-ichiro FUKUMOTO; Naoyuki MAEDA; Yasuhiro HASEGAWA; Tomohito IWASAKI; Marina HOSOTANI; Hiromi UEDA; Takafumi WATANABE, Journal of Veterinary Medical Science, Japanese Society of Veterinary Science, 2024 Oviduct Histopathology of Internal Laying and Egg-Bound Syndrome in Laying Hens, Marina Hosotani; Sohei Hamano; Tomohito Iwasaki; Yasuhiro Hasegawa; Hiromi Ueda; Takafumi Watanabe, Veterinary Sciences, {MDPI} {AG}, 10, (4) 260 - 260, 2023 03 29 The histopathological features of the surgical endometriosis model using systemic autoimmune disease-prone mice., Marina Hosotani; Machiko Akita; Hiromi Ueda; Takafumi Watanabe, The Journal of veterinary medical science, 85, (1) 1 - 8, 2022 11 28 Morphological analysis of cell distribution and network structure via gap junctions in swine corneal stroma., Nobutaka Hoshino; Kazushige Takehana; Marina Hosotani; Kiyokazu Kametani; Tomohito Iwasaki; Yasuhiro Hasegawa; Hiromi Ueda; Takafumi Watanabe, The Journal of veterinary medical science, 84, (12) 1621 - 1632, 2022 10 24 採卵鶏における卵管形態に着目した産卵障害の病態解析, 浜野 爽平; 渡邉 敬文; 岩崎 智仁; 長谷川 靖洋; 植田 弘美; 細谷 実里奈, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 165回, [A3P - 15], 2022 09 Comparing chemiluminescent response and intracellular bactericidal activity of bovine blood neutrophils and monocytes against Salmonella Dublin and Escherichia coli, Satoh Hideyasu; Gondaira Satoshi; Higuchi Hidetoshi; Ueda Hiromi; Nohara Masakatsu; Nagahata Hajime, Japanese Journal of Veterinary Research, 70, (2) 71 - 77, 2022 05 Anatomical features of ossa cordis in the Steller sea lion., Miki Yoshida; Kenjiro Miyoshi; Tomohiko Tajima; Akihiko Wada; Hiromi Ueda; Takanori Kooriyama, The Journal of veterinary medical science, 84(5), (1) 660 - 665, 2022 05 Three-dimensional structural analysis of mitochondria composing each subtype of fast-twitch muscle fibers in chicken., Sachi Makida; Kiyokazu Kametani; Marina Hosotani; Naoki Takahashi; Tomohito Iwasaki; Yasuhiro Hasegawa; Tomohide Takaya; Hiromi Ueda; Takafumi Watanabe, The Journal of veterinary medical science, 84, (6) 809 - 816, 2022 04 14 マウス卵管線毛上皮細胞と排卵卵子の相互作用 加齢と免疫異常は卵子輸送速度を変化させる, 細谷 実里奈; 市居 修; 渡邉 敬文; 植田 弘美; 昆 泰寛, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 164回, [AO - 37], 2021 09 Enhancement Effect of Hepatocellular Mitochondrial Activity in Diabetic Rats with Orally Administered Kampo Medicine, Hiromi Ueda; Yuta Ito; Tomoko Nozaki; Naoki Takahashi; Marina Hosotani; Takafumi Watanabe, The journal of comparative integrative medicine/ Japan, 27, (2) 5 - 12, 2021 03 Unique histological features of the tail skin of cotton rat (Sigmodon hispidus) related to caudal autotomy., Marina Hosotani; Teppei Nakamura; Osamu Ichii; Takao Irie; Yuji Sunden; Yaser Hosny Ali Elewa; Takafumi Watanabe; Hiromi Ueda; Takashi Mishima; Yasuhiro Kon, Biology open, 10, (2) , 2021 02 19 Three‐dimensional ultrastructure reconstruction of tendinous components at the bifurcation of the bovine superficial digital flexor tendon using array and STEM tomographies, Naoki Takahashi; Kiyokazu Kametani; Ryo Ota; Prasarn Tangkawattana; Tomohito Iwasaki; Yasuhiro Hasegawa; Hiromi Ueda; Marina Hosotani; Takafumi Watanabe, Journal of Anatomy, Wiley, 238, (1) 63 - 72, 2021 01 Anatomy and histology of the foramen of ovarian bursa opening to the peritoneal cavity and its changes in autoimmune disease‐prone mice, Marina Hosotani; Osamu Ichii; Teppei Nakamura; Takashi Namba; Md. Rashedul Islam; Yaser Hosny Ali Elewa; Takafumi Watanabe; Hiromi Ueda; Yasuhiro Kon, Journal of Anatomy, Wiley, 238, (1) 73 - 85, 2021 01 Physiological and Pathological Mitochondrial Clearance Is Related to Pectoralis Major Muscle Pathogenesis in Broilers With Wooden Breast Syndrome, Marina Hosotani; Takeshi Kawasaki; Yasuhiro Hasegawa; Yui Wakasa; Maki Hoshino; Naoki Takahashi; Hiromi Ueda; Tomohide Takaya; Tomohito Iwasaki; Takafumi Watanabe, Frontiers in Physiology, Frontiers Media SA, 11, 579 - 579, 2020 06 16 Ultrastructural study of the three-dimensional tenocyte network in newly hatched chick Achilles tendons using serial block face-scanning electron microscopy., Shu Hadate; Naoki Takahashi; Kiyokazu Kametani; Tomohito Iwasaki; Yasuhiro Hasega; Prasarn Tangkawattana; Takeshi Kawasaki; Hiromi Ueda; Marina Hosotani; Takafumi Watanabe, The Journal of veterinary medical science, 82, (7) 948 - 954, 2020 05 15 3次元電子顕微鏡を用いたアキレス腱の腱細胞の細胞質突起の立体構造解析, 羽立 洲; 高橋 直紀; 細谷 実里奈; 植田 弘美; 渡邉 敬文, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 162回, 334 - 334, 2019 08 ウシ浅趾屈筋腱分岐部のコラーゲン細線維の走行と超微細構造の3次元解析, 高橋 直紀; 亀谷 清和; 大多 亮; 細谷 実里奈; 植田 弘美; 渡邉 敬文, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 162回, 334 - 334, 2019 08 Fibrillar architecture at three different sites of the bovine superficial digital flexor tendon, Naoki Takahashi; Takuya Hirose; Jun A. Minaguchi; Hiromi Ueda; Prasarn Tangkawattana; Kazushige Takehana, Journal of Veterinary Medical Science, Japanese Society of Veterinary Science, 80, (3) 405 - 412, 2018 Natural larval Echinococcus multilocularis infection in a Norway rat, Rattus norvegicus, captured indoors in Hokkaido, Japan, Shin-ichiro Fukumoto; Seitaro Yamada; Manato Fushikida; Shotaro Toyada; Tomotaka Nishikawa; Hidetoshi Higuchi; Hiroshi Ueno; Hiromi Ueda; Hiromu Sugiyama; Yasuyuki Morishima, JOURNAL OF VETERINARY MEDICAL SCIENCE, JAPAN SOC VET SCI, 79, (11) 1857 - 1860, 2017 11 Remodeling of rat sir stormal-vascular cells to brite/beige adipocytes by prolyl-hydroxyproline, Jun A. Minaguchi; Sakino Ogata; Naoki Takahashi; Takuya Hirose; Hiromi Ueda; Kazushige Takehana, JOURNAL OF VETERINARY MEDICAL SCIENCE, JAPAN SOC VET SCI, 79, (3) 547 - 553, 2017 03 Anti-diabetic activities of traditional Chinese herbal medicine in streptozotocin-induced diabetic rats, Tomoko Nozaki; Jun Minaguchi; Kazushige Takehana; Hiromi Ueda, Okajimas Folia Anatomica Japonica, Okajima Foria Anatomica Yaponika Henshubu, 93, (4) 111 - 118, 2017 02 01 Anti-diabetic activities of traditional Chinese herbal medicine in streptozotocin-induced diabetic rats, Tomoko Nozaki; Jun Minaguchi; Kazushige Takehana; Hiromi Ueda, Okajima Folia Anatomia Japonica, 79, 574 - 553, 2017 Morphometric analysis of growing tenocytes in the superficial digital flexor tendon of piglets, Naoki Takahashi; Prasarn Tangkawattana; Yoshiki Ootomo; Takuya Hirose; Jun Minaguchi; Hiromi Ueda; Michi Yamada; Kazushige Takehana, Journal of Veterinary Medical Science, Japanese Society of Veterinary Science, 79, (12) 1960 - 1967, 2017 Effectiveness of Isoprothiolane in Preventing Fatty Liver in Dairy Cows, Minaguchi, J; Miura, M; Oikawa, S; Handa, F; Haraguchi, T; Miyamoto-Ohno, K; Ito, Y; Hosaka, Y; Ueda, H; Takehana, K, The Open Veterinary Science Journal, 7, 7 - 11, 2013 01 Histological differences of skin among three body regions in male and female Hokkaido sika deer (Cervus nippon yesoensis), Jun A. Minaguchi; Ayako Abe; Hiromi Ueda; Prasarn Tangkawattana; Kazushige Takehana, Okajimas Folia Anatomica Japonica, 89, (1) 1 - 5, 2012 05 Concerted and adaptive alignment of decorin dermatan sulfate filaments in the graded organization of collagen fibrils in the equine superficial digital flexor tendon., Takafumi Watanabe; Yasutada Imamura; Daisuke Suzuki; Yoshinao Hosaka; Hiromi Ueda; Kohzy Hiramatsu; Kazushige Takehana, Journal of anatomy, 220, (2) 156 - 163, 2012 02 Efficacy of self-assembled hydrogels composed of positively or negatively charged peptides as scaffolds for cell culture., Nagayasu A; Yokoi H; Minaguchi JA; Hosaka YZ; Ueda H; Takehana K, Journal of biomaterials applications, 26, (6) 651 - 665, 2012 02 Morphological and biochemical analysis of intact and opaque cornea in dogs., Hirooka M; Igarashi O; Nagayasu A; Minaguchi J; Hosaka YZ; Ueda H; Tangkawattana P; Takehana K, Okajimas folia anatomica Japonica, 87, (2) 59 - 64, 2010 08 Comparative study of the properties of tendinocytes derived from three different sites in the equine superficial digital flexor tendon., Hosaka YZ; Uratsuji T; Ueda H; Uehara M; Takehana K, Biomedical research (Tokyo, Japan), 31, (1) 35 - 44, 2010 02 Comparative study of the characteristics and properties of tendinocytes derived from three tendons in the equine forelimb, Yoshinao Z. Hosaka; Hideyuki Takahashi; Takehiro Uratsuji; Prasarn Tangkawattana; Hiromi Ueda; Kazushige Takehana, TISSUE & CELL, CHURCHILL LIVINGSTONE, 42, (1) 9 - 17, 2010 02 A Preliminary Study of the Administrative Effect of Collagen Peptides Derived from Salmon Skin on Dermis Around Bovine Hooves, HOSAKA Yoshinao; GOTOH Chihiro; ABE Kei; NAGAYASU Aya; UEDA Hiromi; TAKEHANA Kazushige, J.Jpn.Vet.Med.Assoc., 日本獸医師会, 63, (1) 38 - 43, 2010 01 20 Site-Dependent Differences in Collagen Lamellae in the Corneal Substantia Propria of Beagle Dogs, Aya Nagayasu; Tomoe Hirayanagi; Yuji Tanaka; Prasarn Tangkawattana; Hiromi Ueda; Kazushige Takehana, JOURNAL OF VETERINARY MEDICAL SCIENCE, JAPAN SOC VET SCI, 71, (9) 1229 - 1231, 2009 09 Morphological changes of the jejunum villi to different diets, HOSHINO Yuji; TAMAKI Kazuyuki; INAYAMA Ichinari; UENO Mitsutoshi; NAGAYASU Aya; HOSAKA Yoshinao; UEDA Hiromi; TAKEHANA Kazushige, Nihon Chikusan Gakkaiho, 公益社団法人 日本畜産学会, 80, (2) 171 - 177, 2009 05 25 Morphological changes of the jejunum villi to di erent diets, 星野 佑治; 玉置 和之; 稲山 一成; 上野 光敏; 永易 彩; 保坂 善真; 植田 弘美; 竹花 一成, 日本畜産学会報 = The Japanese journal of zootechnical science, 日本畜産学会, 80, (2) 171 - 177, 2009 05 Effect of Collagen Oligopeptide Injection on Rabbit Tenositis, Hiromi Ueda; Natsuko Meguri; Jun Minaguchi; Takafumi Watanabe; Aya Nagayasu; Yoshinao Hosaka; Prasarn Tangkawattana; Yasuo Kokai; Kazushige Takehana, JOURNAL OF VETERINARY MEDICAL SCIENCE, JAPAN SOC VET SCI, 70, (12) 1295 - 1300, 2008 12 Morphology of mesenteric arteries in horse having endotoxemia-related colic, HIDAKA Shuhei; UEDA Hiromi; HOSAKA Yoshinao; TAKEHANA Kazushige, Journal of the College of Dairying. Natural science, 酪農学園大学, 33, (1) 99 - 104, 2008 10 Effect of prolyl hydroxyproline on the differentiation of mouse 3T3-L1 preadipocytes in vitro, SAWAYA Satoe; KOYAMA Yoh-ichi; NAGAYASU Aya; HOSAKA Yoshinao; UEDA Hiromi; MAFUNE Naoki; TAKEHANA Kazushige, Journal of the College of Dairying. Natural science, 酪農学園大学, 33, (1) 13 - 18, 2008 10 Morphological analysis of corneal opacity in Shiba dog with GM1 gangliosidosis., Nagayasu A; Nakamura T; Yamato O; Tsuzuki K; Hosaka Y; Ueda H; Tangkawattana P; Takehana K, The Journal of veterinary medical science, 70, (9) 881 - 886, 2008 09 A preliminary study of direct application of atelocollagen into a wound lesion in the dog cornea, Aya Nagayasu; Yoshinao Hosaka; Ayako Yamasaki; Keiko Tsuzuki; Hiromi Ueda; Tomoaki Honda; Kazushige Takehana, CURRENT EYE RESEARCH, INFORMA HEALTHCARE, 33, (9) 727 - 735, 2008 The modulation of collagen fibril assembly and its structure by decorin: an electron microscopic study., Iwasaki S; Hosaka Y; Iwasaki T; Yamamoto K; Nagayasu A; Ueda H; Kokai Y; Takehana K, Arch. Histol. Cytol., 71, (1) 37 - 44, 2008 Graded arrangement of collagen fibrils in the equine superficial digital flexor tendon, Takafumi Watanabe; Yasutada Imamura; Yoshinao Hosaka; Hiromi Ueda; Kazushige Takehana, Connective Tissue Research, 48, (6) 332 - 337, 2007 11 01 Characteristics of collagen fibrils in the entire equine superficial digital flexor tendon, Masaru Sese; Hiromi Ueda; Takafumi Watanabe; Etsuko Yamamoto; Yoshinao Hosaka; Prasarn Tangkawattana; Kazushige Takehana, Okajimas Folia Anatomica Japonica, 84, (3) 111 - 114, 2007 11 Effect of heat on synthesis of gelatinases and pro-inflammatory cytokines in equine tendinocytes., Hosaka Y; Ozoe S; Kirisawa R; Ueda H; Takehana K; Yamaguchi M, Biomedical research (Tokyo, Japan), 27, (5) 233 - 241, 2006 10 Effects of ingestion of collagen peptide on collagen fibrils and Glycosaminoglycans in the dermis, Naoya Matsuda; Yoh-ichi Koyama; Yoshinao Hosaka; Hiromi Ueda; Takafumi Watanabe; Takayuki Araya; Shinkichi Irie; Kazushige Takehana, JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, CENTER ACADEMIC PUBL JAPAN, 52, (3) 211 - 215, 2006 06 Morphological, biochemical and mechanical features of the cranial cruciate and lateral collateral ligaments in dogs, Hiromi Ueda; Takuya Matsukawa; Takafumi Watanabe; Yoshinao Hosaka; Kazushige Takehana, Okajimas Folia Anatomica Japonica, 83, (1) 25 - 31, 2006 Control of the collagen fibril diameter in the equine superficial digital flexor tendon in horses by decorin, T Watanabe; Y Hosaka; E Yamamoto; H Ueda; K Sugawara; H Takahashi; K Takehana, JOURNAL OF VETERINARY MEDICAL SCIENCE, JAPAN SOC VET SCI, 67, (9) 855 - 860, 2005 09 Distribution of TNF receptors and TNF receptor-associated intracellular signaling factors on equine tendinocytes in vitro., Yoshinao Hosaka; Yurie Sakamoto; Rikio Kirisawa; Takafumi Watanabe; Hiromi Ueda; Kazushige Takehana; Mamoru Yamaguchi, The Japanese journal of veterinary research, 52, (3) 135 - 144, 2004 11 Morphological study of the Golgi tendon organ in equine superficial digital flexor tendon., Takafumi Watanabe; Yoshinao Hosaka; Etsuko Yamamoto; Hiromi Ueda; Prasarn Tangkawattana; Kazushige Takehana, Okajimas folia anatomica Japonica, 81, (2-3) 33 - 37, 2004 08 Effect of beta-aminopropionitrile and hyaluronic acid on repair of collagenase-induced injury of the rabbit Achilles tendon, E Yamamoto; D Hata; A Kobayashi; H Ueda; P Tangkawattana; M Oikawa; K Takehana, JOURNAL OF COMPARATIVE PATHOLOGY, ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD, 126, (2-3) 161 - 170, 2002 02 Characteristics of dorsal lingual papillae of the Bactrian camel (Camelus bactrianus), Eerdunchaolu; K Takehana; E Yamamoto; A Kobayashi; G Cao; Baiyin; H Ueda; P Tangkawattana, ANATOMIA HISTOLOGIA EMBRYOLOGIA-JOURNAL OF VETERINARY MEDICINE SERIES C, BLACKWELL WISSENSCHAFTS-VERLAG GMBH, 30, (3) 147 - 151, 2001 06 Immunohistochemical study of the distribution of endocrine cells in the gastrointestinal tract of the camel (Camelus bactrianus), DVM Eerdunchaolu; K Takehana; A Kobayashi; J Yamada; H Ueda; Baiyin; GF Cao; M Abe, EUROPEAN JOURNAL OF MORPHOLOGY, SWETS ZEITLINGER PUBLISHERS, 39, (1) 57 - 63, 2001 02 イヌの角膜成熟に関する形態学的、生化学的解析, 石井陽子; 山本悦子; 植田弘美; 竹花一成, 日本獣医師会雑誌, 54, (11) 843 - 846, 2001 Characteristics of Dorsal Lingual Papillae of the Bactrian Camel (Camelus Bactrianus), EERDUNCHAOLU; K. TAKEHANA; E. YAMAMOTO; A. KOBAYASHI; G. CAO; BAIYIN; H.UEDA; P. TANGKAWATTANA, Anat. Hist. Emb, 30, 1 - 5, 2001 ウマの実験的浅指屈腱炎に対する薬物治療効果の評価, 山本 悦子; 小林 中; 山下 和人; 岩佐 憲二; 吉原 豊彦; 植田 弘美; 竹花 一成, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 128回, 202 - 202, 1999 09
Books etc
獣医組織学実習マニュアル 第2版, 五味浩司; 吉岡一機; 坂上元栄, 第2章 筋組織, 学窓社, 2022 09 , 9784873627854 獣医組織学第八版, 日本獣医解剖学会 編, Chapter 8 脈管系；Chapter 9 リンパ器官, 学窓社, 2020 03 獣医組織学第七版, 植田 弘美, Chapter8 脈管系, 学窓社, 2017 03 書いて覚える塗って身につく動物解剖学ノート, 植田 弘美, Unit 13 - Unit 20, 講談社, 2016 03
Conference Activities & Talks
ブタ角膜固有質における細胞分布とギャップ結合によるネットワーク構造の形態学的解析, 星野信隆; 細谷実里奈; 植田弘美; 渡邉敬文, 令和4年度 日本顕微鏡学会 北海道支部学術講演会 北海道産海水魚における脊椎骨の組織学的解析と分類, 祖父江尚哉; 古田智絵; 山田加一朗; 吉川修司; 細谷実里奈; 渡邉敬文; 植田弘美, 令和4年度 日本顕微鏡学会 北海道支部学術講演会 Pathological role of hedgehog signaling in maintenance of the murine oviductal ciliated epithelium, Marina Hosotani; Osamu Ichii; Takashi Namba; Teppei Nakamura; Yasuhiro Hasegawa; Md. Abdul Masum; Takafumi Watanabe; Hiromi Ueda; Yasuhiro Kon, The 8th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 採卵鶏における卵管形態に着目した産卵障害の病態解析, 浜野 爽平; 渡邉 敬文; 岩﨑 智仁; 長谷川 靖洋; 植田 弘美; 細谷 実里奈, 第165回日本獣医学会学術集会 自己免疫疾患モデルマウスを使用した子宮内膜症の病態解析, 秋田真知子; 渡邉敬文; 植田弘美; 細谷実里奈, 第165回日本獣医学会学術集会 甘草の過剰摂取に伴うラット腎臓の形態変化, 李垠錫; 細谷実里奈; 渡邉敬文; 植田弘美, 令和3年度日本顕微鏡学会北海道支部学術講演会 ニワトリ速筋線維の各サブタイプを構成するミトコンドリアの三次元 構造解析, 牧田紗智; 岩﨑智仁; 長谷川靖洋; 細谷実里奈; 亀谷清和; 髙橋直紀; 植田弘美; 渡邉 敬文, 令和3年度日本顕微鏡学会北海道支部学術講演会 甘草過剰摂取により派生する肝線維化および細胆管反応の形態解析, 的場聖礼; 細谷実里奈; 渡邉敬文; 植田弘美, 令和3年度日本顕微鏡学会北海道支部学術講演会 マウス卵管線毛上皮細胞と排卵卵子の相互作用ー加齢と免疫異常は卵子輸送速度を変化させるー, 細谷実里奈; 市居修; 渡邉敬文; 植田弘美; 昆奏寛, 第164回日本獣医学会学術集会 ニワトリ外側腸脛骨筋ミトコンドリアの三次元構造解析, 牧田紗智; 岩崎智仁; 長谷川靖洋; 細谷実里奈; 亀谷清和; 高橋直樹; 植田弘美; 渡邉敬文, 第164回日本獣医学会学術集会 ラットの皮下に存在するFasciaの形態学的観察, 佐野日向子; 岩崎智仁; 長谷川靖洋; 細谷実里奈; 植田弘美; 渡邉敬文, 第164回日本獣医学会学術集会 北海道産魚類における脊柱の組織学的検討, 山口慧; 古田智絵; 吉川修司; 山田加一朗; 渡邉敬文; 細谷実里奈; 植田弘美, 日本顕微鏡学会第63回シンポジウム 北海道支部学術講演会 マウス卵管の線毛におけるヘッジホッグシグナル伝達経路の発現, 細谷実里奈; 市居修; 中村鉄平; 長谷川靖洋; Md. Abdul Masum; 渡邉敬文; 植田弘美; 昆泰寛, 第163回日本獣医学会学術集会 コットンラットの尾の組織学的特徴および自切機序の解析, 日本獣医学会学術集会講演要旨集, 第163回日本獣医学会学術集会 バーチャル電子顕微鏡データの作製方法と応用について, 渡邉 敬文; 高橋 直紀; 細谷 実里奈; 植田 弘美; 岩崎 智仁; 亀谷 清和, 第163回日本獣医学会学術集会講演要旨集 ドライエイジングによる筋組織の形態変化の解析, 小原千佳、高橋直紀、細谷実里奈、渡邉敬文、植田弘美, 日本顕微鏡学会北海道支部学術講演会 ウシ浅趾屈筋腱分岐部の微細構造の三次元解析, 高橋 直紀; 亀谷 清和; 大多 亮; 植田 弘美; 渡邉 敬文, 日本顕微鏡学会北海道支部学術講演会 マウスの卵巣囊－腹腔連絡路（卵巣囊孔）, 細谷実里奈、市居修、中村鉄平、難波貴志、Ali Elewa Yaser Hosny、渡邉敬文、植田弘美、昆泰寛, 第162回日本獣医学会学術集会 ウシ浅趾屈筋腱分岐部のコラーゲン細線維の走行と超微細構造の３次元解析, 高橋直紀、亀谷清和、大多亮、細谷実里奈、植田弘美、渡邉敬文, 第162回日本獣医学会学術集会 ３次元電子顕微鏡を用いたアキレス腱の腱細胞の細胞質突起の立体構造解析, 羽立洲、高橋直紀、細谷実里奈、植田弘美、渡邉敬文, 第162回日本獣医学会学術集会 腱分岐部の微細構造の3次元化, 髙橋直紀、亀谷清和、大多亮、植田弘美、渡邉敬文, 日本顕微鏡学会第75回学術講演会 新型エーラス・ダンロス症候群の皮膚病理解析から得た脆弱な皮膚のcollagen fibrilと糖鎖の構造, 広瀬 拓哉; 古症 知巳; 高橋 有希; 美名口 順; 高橋 直紀; 植田 弘美; 竹花 一成, 日本顕微鏡学会北海道支部学術講演会 糖尿病の発現に伴う肝臓の組織学的変化, 植田 弘美; 森井 俊貴; 平賀 武夫, 日本顕微鏡学会北海道支部学術講演会 糖尿病誘発ラットにおける肝線維化の形態学的観察, 植田 弘美; 森井 俊貴; 平賀 武夫, 日本解剖学会第63回東北・北海道連合支部学術集会 次亜塩素酸ナトリウムを用いた骨格標本作製の試み, 岩崎 聖; 中尾 伊吹; 平賀 武夫; 植田 弘美, 日本解剖学会第62回東北・北海道連合支部学術集会 糖尿病ラットに発現される肝臓の形態学的観察, 植田 弘美; 平賀 武夫, 日本解剖学会第62回東北・北海道連合支部学術集会
Works
ブタ脾臓の微細血管系の陰性荷電について, 2001 Anionic sites in the blood vessels of the pig spleen, 2001 十二指腸分泌物の分子生物学的解析, 1999 フタコブラクダ(Camelus Bactrianus)の舌の形態特徴, 1999 脾臓微細血管系の陰性荷電について, 1999 Molecular biological analysis of secretion in duodenal glands, 1999 Morphological feature in the camel (Camelus Bactrianus) tongue, 1999 Anionic sites in splenic blood vessels, 1999 ラット脾臓の血管内皮細胞および脾索細網細胞の形質膜糖衣における陰性荷電の電顕組織化学的研究, 1998 ラット大動脈のグリコサミノグリカンの電顕組織化学における三重酵素消化法の効果に関する研究, 1998 Electron microscopic histochemical studies of anionic sites on free surface of endothelial cells of blood vessels and reticular cells of splenic cord in rat spleen, 1998 Effects of a triple enzyme digestion method on a diamine reaction for glycosaminoglycans of the rat aorta in electron microscopy, 1998 モルモット脾臓血管内皮における糖質の電顕組織化学的研究, 1993 Electron microscopic histochemical studies on carbohydrates in the endothelium of the guinea pig splenic blood vessels, 1993 尿素処理超薄切片を使用する包埋後法によるN-硫酸複合糖質の検出法, 1991 A postembedding method using urea-unmasked ultrathin sections fot the electron microscopic detection of N-sulfated glycoconjugates, 1991 ラット脾臓血管系内皮細胞における糖質の電顕細胞化学的研究, 1990 Electron microscopic cytochemistry of carbohydrates in the endothelical cells lining the splenic blood vessels of the rat, 1990
Misc
ラットの皮下に存在するFasciaの形態学的観察, 佐野日向子; 岩崎智仁; 長谷川靖洋; 細谷実里奈; 植田弘美; 渡邉敬文, 日本獣医学会学術集会講演要旨集, 164th (CD-ROM), 2021 ニワトリ外側腸脛骨筋ミトコンドリアの三次元構造解析, 牧田紗智; 岩崎智仁; 長谷川靖洋; 細谷実里奈; 亀谷清和; 高橋直紀; 植田弘美; 渡邉敬文, 日本獣医学会学術集会講演要旨集, 164th (CD-ROM), 2021 ブロイラーのWooden Breast症における線維化メカニズムの研究, 益田侑空; 細谷実里奈; 岩崎智仁; 長谷川靖洋; 川崎武志; 植田弘美; 渡邉敬文, 日本獣医学会学術集会講演要旨集, 164th (CD-ROM), 2021 ニワトリ速筋線維の各サブタイプを構成するミトコンドリアの三次元構造解析, 牧田紗智; 岩崎智仁; 長谷川靖洋; 細谷実里奈; 亀谷清和; 高橋直紀; 植田弘美; 渡邉敬文, 日本顕微鏡学会北海道支部学術講演会講演要旨集, 2021 (CD-ROM), 2021 マウス卵管線毛上皮細胞と排卵卵子の相互作用-加齢と免疫異常は卵子輸送速度を変化させる-, 細谷実里奈; 市居修; 渡邉敬文; 植田弘美; 昆泰寛, 日本獣医学会学術集会講演要旨集, 164th (CD-ROM), 2021 コットンラットの尾の組織学的特徴および自切機序の解析, 中村鉄平; 中村鉄平; 細谷実里奈; 市居修; 入江隆夫; 入江隆夫; 寸田祐嗣; ELEWA Yaser Hosny Ali; 三嶋隆; 渡邉敬文; 植田弘美; 昆泰寛, 日本獣医学会学術集会講演要旨集, 163rd, 2020 マウス卵管の線毛におけるヘッジホッグシグナル伝達経路の発現, 細谷実里奈; 市居修; 中村鉄平; 中村鉄平; 長谷川靖洋; MASUM Md. Abdul; 渡邉敬文; 植田弘美; 昆泰寛, 日本獣医学会学術集会講演要旨集, 163rd, 2020 マウスの卵巣嚢-腹腔連絡路(卵巣嚢孔), 細谷実里奈; 市居修; 中村鉄平; 中村鉄平; 難波貴志; YASER HOSNY Ali Elewa; 渡邉敬文; 植田弘美; 昆泰寛, 日本獣医学会学術集会講演要旨集, 162nd, 2019 蝸牛の種差に関する鋳型樹脂標本を用いた研究, 城戸 大樹; 髙橋 直紀; 広瀨 拓哉; 福冨 彰; 植田 弘美; 白石 治恵; 竹花 一成, Research of One Health, 2017 06 豚の肝小葉の経時的な構造変化, 美案口 順; 山本かおる; 植田弘美; 竹花一成, 酪農学園大学紀要, 37, 2013 04 Comparative study of the characteristics and properties of tendinocytes derived from three tendons in the equine forelimb (vol 42, pg 9, 2010), Yoshinao Z. Hosaka; Hideyuki Takahashi; Takehiro Uratsuji; Parasarn Tangkawattana; Hiromi Ueda; Kazushige Takehana, TISSUE & CELL, 42, (4) 273 - 273, 2010 08 , 10.1016/j.tice.2010.08.001 角膜実質の再生医療 1.ブタ角膜実質細胞の培養法の検討, 永易彩; 角出泰造; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 145th, 2008 03 07 Active Expression of Matrix Metalloproteinase-13 mRNA in the Granulation Tissue of Equine Superficial Digital Flexor Tendinitis, NOMURA Masayuki; HOSAKA Yoshinao; KASASHIMA Yoshinori; UEDA Hiromi; TAKEHANA Kazushige; KUWANO Atsutoshi; ARAI Katsuhiko, Journal of Veterinary Medical Science, 69, (6) 637 - 639, 2007 06 25 , The DNA microarray analysis for matrix metalloproteinase (MMP)-related mRNA expression in equine superficial digital flexor tendinitis indicated that mRNA level of MMP-13 was apparently up-regulated in the tendinitis as compared to normal tendon. <i>In situ</i> hybridization also revealed that fibroblastic cells proliferated in the granulation tissue generated in the tendinitis actively expressed MMP-13 mRNA. On the other hand, in normal tendon, a few fibroblastic cells and vascular components lied in the endotenon barely expressed its mRNA, but other cellular components in the tendon bundle were not positively hybridized. As mentioned above, MMP-13 but not other collagenases or gelatinases, may play an important role in tendon injuries in the racehorses.<br> コラーゲン形態形成におけるデコリンの役割, 岩崎俊輔; 保坂善真; 植田弘美; 小海康夫; 竹花一成, 解剖学雑誌, 82, (2) 76 - 76, 2007 06 01 3T3‐L1脂肪前駆細胞の分化による脂肪蓄積と形態変化, 沢谷里江; 植田弘美; 保坂善真; 真船直樹; 新谷隆行; 小山洋一; 竹花一成, 解剖学雑誌, 82, (2) 76 - 76, 2007 06 01 ノックダウン細胞を用いた,デコリンとコラーゲン細繊維の相互関連の解析, 保坂善真; 岩崎俊輔; 植田弘美; 小海康夫; 竹花一成, 解剖学雑誌, 82, (2) 76 - 76, 2007 06 01 筋腱接合部における腱の構造特性について, 渡辺敬文; 今村保忠; 保坂善真; 植田弘美; 竹花一成, 日本顕微鏡学会北海道支部学術講演会プログラム・予稿集, 2006, 2007 02 コラーゲンペプチドの経口摂取がブタ皮膚の細胞外マトリックスに及ぼす効果, 松田直也; 保坂善真; 植田弘美; 竹花一成, 日本顕微鏡学会北海道支部学術講演会講演要旨集, 2007, 2007 イヌの食道における粘膜筋板の形態学的研究, 吉田愛; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 142nd, 2006 08 31 筋腱接合部のコラーゲン細線維の構造特性について, 渡辺敬文; 今村保忠; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 142nd, 2006 08 31 イヌ顎関節の軟骨組織に含まれる複合糖質の組織化学的研究, 広部真智; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 142nd, 2006 08 31 コラーゲンペプチド投与によるウシの蹄冠および趾間皮膚に対する効果, 阿部敬; 永易彩; 渡辺敬文; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 142nd, 2006 08 31 腱炎に対するコラーゲンオリゴペプチドの効果, 巡夏子; 美名口順; 渡辺敬文; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 142nd, 2006 08 31 ウマ浅指屈筋腱細胞の性状は存在部位によって異なる, 浦辻雄央; 保坂善真; 桐沢力雄; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 142nd, 2006 08 31 実験的脂肪肝牛の肝類洞内皮細胞下基底膜の形態的変化, 宮本和吉; 及川伸; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 142nd, 2006 08 31 趾乳頭腫症Papillomatous Digital Dermatitis(PDD)の皮膚構造と痛覚過敏の原因について, 田中健介; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 142nd, 2006 08 31 皮膚真皮のコラーゲン線維とグリコサミノグリカンに対するコラーゲンペプチド摂取の効果, 松田 直也; 小山 洋一; 保坂 善真; 植田 弘美; 渡辺 敬文; 新谷 隆行; 入江 伸吉; 竹花 一成, ビタミン, 80, (8) 433 - 433, 2006 08 , https://search-tp.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2006&ichushi_jid=J01262&link_issn=&doc_id=20060831370018&doc_link_id=%2Fcr9vitam%2F2006%2F008008%2F020%2F0433-0433%26dl%3D2&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fcr9vitam%2F2006%2F008008%2F020%2F0433-0433%26dl%3D2&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_5.gif クリプトスポリジウム人工感染子牛に関する治療・予防効果‐小腸粘膜での形態学的評価‐, 竹花一成; 沢谷里江; 保坂善真; 植田弘美; 小岩政照, 北海道獣医師会雑誌, 50, (8) 297 - 41, 2006 08 01 Structure and component alteration of rabbit Achilles tendon in tissue culture, Y Hosaka; H Ueda; T Yamasaki; D Suzuki; N Matsuda; K Takehana, BIOMEDICAL RESEARCH-TOKYO, 26, (6) 279 - 286, 2005 12 , 10.2220/biomedres.26.279, The aim of this study was to investigate alterations of cultured tendon tissues to determine whether tissue culture is a useful method for biological analyses of the tendon. Tendon tissues for tissue culture were isolated from Achilles tendons of rabbits. The tendon segments were placed one segment per well and incubated in growth medium consisting of Dullbecco's modified Eagle's medium supplemented with 5% fetal bovine serum at 37 degrees C in a humidified atmosphere with 5% CO2 for various periods. The alignment of collagen fibrils was preserved for 48 h, but tendon structure has disintegrated at 96 h. Alcian blue staining and gelatine zymography revealed that proteoglycan markedly diminished and that matrix metalloproteinase (MMPs) activity was upregulated sharply at 72 and 96 h. The ratio of collagen fibrils with large diameter had increased and the mean diameter and mass average diameter value had reached maximum at 48 h. The values then decreased and mean diameters at 72 and 96 h were significantly different from that at 48 h. At 96 h, the ratio of collagen fibrils with small diameters had increased and collagen fibrils with large diameters had disappeared. These findings indicate that structural alteration is possible to be induced by disintegration of collagen fibrils and disappearance of glycosaminoglycans from extracellular matrix (ECM), subsequent of upregulation of MMPs activity. Although the study period is limited, the tissue culture method is available for investigating cell-ECM interaction in tendons. 腱の状態によるTNFαとTNF-R1を介する細胞内シグナル伝達因子の分布と発現の差違, 保坂 善真; 桐澤 力雄; 植田 弘美; ほか, The journal of veterinary medical science, 67, (10) 985 - 991, 2005 10 柴犬のGM1ガングリオシドーシスにおける角膜混濁機序について, 中村泰斗; 大和修; 都築圭子; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 140th, 2005 08 31 ウマの鎖骨画における形態学的研究, 植田弘美; 寺井洋子; 渡辺敬文; 保坂善真; 竹花一成, 日本獣医学会学術集会講演要旨集, 140th, 2005 08 31 コラーゲンペプチドの経口摂取がブタ皮膚の細胞外マトリックスに及ぼす効果, 松田直也; 保坂善真; 植田弘美; 小山洋一; 入江伸吉; 竹花一成, 日本獣医学会学術集会講演要旨集, 140th, 2005 08 31 SHNマウスにおける子宮炎好発とVitamin Aとの関係, 山元明子; 竹花一成; 保坂善真; 植田弘美, 日本獣医学会学術集会講演要旨集, 140th, 2005 08 31 サケ皮膚由来I型コラーゲンゲルを用いたけん細胞培養の展開, 龍尾幸治; 保坂善真; 植田弘美; 真船直樹; 鈴木健; 竹花一成, 日本獣医学会学術集会講演要旨集, 140th, 2005 08 31 けん変性過程での熱,炎症性サイトカインおよびゼラチナーゼの関連, 保坂善真; 尾添紗知子; 桐沢力雄; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 140th, 2005 08 31 ウマ浅指屈筋けんのコラーゲン細線維直径を変化させる分子要因について, 渡辺敬文; 今村保忠; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 140th, 2005 08 31 若齢期のウマにおける伸筋けんおよび屈筋けんの組織学的変化, 山崎維嗣; 渡辺敬文; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 140th, 2005 08 31 The Molecular Marker NTx Reflecting Changes in Tissue Remodeling Associated with Injury to the Equine Superficial Digital Flexor Tendon, HOSAKA Yoshinao; HIGUCHI Hidetoshi; ONAGA Takenori; NAGATA Hiroshi; KAMIKURA Tomoki; MISUMI Kazuhiro; UEDA Hiromi; Kazushige, Journal of the Japan Veterinary Medical Association, 58, (3) 175 - 179, 2005 03 20 , 骨の代謝マーカーの一つであるI型コラーゲン架橋N-末端テロペプチド濃度（Nx）が馬の腱損傷後の治癒過程をモニタリングするマーカーとして有用であるかを検討した。血清中Nx濃度は腱損傷のない正常群と比較して、腱炎の急性期および亜急性期群で有意に高い値を示した。しかし、慢性期群と正常群間とのNx値には有意差が認められなかった。腱炎による腱のI型コラーゲンの分解は亜急性期までで、慢性期以降では腱組織の再構築が開始されることから、血清中Nx濃度は腱炎によるコラーゲン分解を鋭敏に反映していると考えられた。 RNAi法によるデコリンの遺伝子抑制がコラーゲン細線維のサイズと走行性に与える影響, 保坂善真; 小海康夫; 渡辺敬文; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 139th, 2005 03 01 ウマ浅指屈筋けんのコラーゲン細線維の形態は部位によって変化する, 渡辺敬文; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 139th, 2005 03 01 膝関節の靭帯の構造特性, 竹花一成; 大友由佳子; 松川拓也; 渡辺敬文; 保坂善真; 植田弘美, 日本獣医学会学術集会講演要旨集, 139th, 2005 03 01 Effects of ingestion of collagen peptide on collagen fibrils and glycosaminoglycans in Achilles tendon, Jun Minaguchi; Yoh-Ichi Koyama; Natsuko Meguri; Yoshinao Hosaka; Hiromi Ueda; Masashi Kusubata; Arisa Hirota; Shinkichi Irie; Naoki Mafune; Kazushige Takehana, Journal of Nutritional Science and Vitaminology, 51, (3) 169 - 174, 2005 , 10.3177/jnsv.51.169, In order to investigate whether the oral ingestion of collagen peptide affects the extracellular matrix of tendon, two doses (0.2 g/kg and 1.0 g/kg body weight) were orally administered daily for 56 d to a rabbit, and both the size of collagen fibrils and the amount of glycosaminoglycans in the Achilles tendon were measured in comparison with those in a rabbit fed with a control protein, lactalbumin, or water alone. Ingestion of collagen peptide or lactalbumin induced a significant increase in collagen fibril diameter and a decrease in fibril density except for a high dose of lactalbumin compared with the water control. A histogram pattern of fibril diameter in a high dose of collagen peptide showed a peak at 160-180 nm, which was not observed in other groups. However the percentage of diameters over 200 nm was the lowest in this group but highest in the low-dose group of collagen peptide. The mean fibril diameter and mass average diameter of a high dose of collagen peptide were significantly smaller than those in a low dose. The amount of dermatan sulphate increased in the high-dose groups, while the amount of hyaluronic acid decreased in rabbits fed with collagen peptide or lactalbumin at either dose. These results suggest that the ingestion of collagen peptide affects the size of collagen fibrils and composition of glycosaminoglycans in the Achilles tendon and thus may improve the mechanical properties of the Achilles tendon. Mechanism of cell death in inflamed superficial digital flexor tendon in the horse, Y Hosaka; H Teraoka; E Yamamoto; H Ueda; K Takehana, JOURNAL OF COMPARATIVE PATHOLOGY, 132, (1) 51 - 58, 2005 01 , 10.1016/j.jcpa.2004.06.006, The aim of the present study was to clarify the presence and determine the role of apoptosis in the degenerative process of the superficial digital flexor tendon (SDFT) in the horse. Samples were obtained from normal and inflamed SDFTs of horses. To detect apoptosis and to identify apoptotic cells, the samples were subjected to immunohistochemical labelling and Western blot analysis. Although a large number of cells in degenerate areas showed positive reactions with caspase-3 and single stranded DNA antibodies, cells in normal tendon samples showed very weak reactions. Excessive apoptosis was confirmed by the results of Western blot analysis, which showed a significant increase in activated caspase-3 protein in the inflamed SDFTs, suggesting that apoptosis occurred in the tendinocytes via a caspase-3-dependent pathways This is the first report of excessive apoptosis in inflamed SDFT of the horse. The results indicate that apoptosis may play an important role in the process of degeneration of the tendon as well as other tissues. (C) 2004 Elsevier Ltd. All rights reserved. RNAi法によるデコリンの産生抑制がコラーゲン細線維のサイズと走行性に与える影響, 保坂善真; 小海康夫; 渡辺敬文; 植田弘美; 竹花一成, 日本結合組織学会学術大会抄録集, 37th, 2005 ウマの浅指屈筋けんにおけるコラーゲン細線維直径の調節, 渡辺敬文; 保坂善真; 山本悦子; 植田弘美; 竹花一成, 日本結合組織学会学術大会抄録集, 37th, 2005 Differences in Tumor Necrosis Factor (TNF)α and TNF Receptor-1-Mediated Intracellular Signaling Factors in Normal, Inflamed and Scar-Formed Horse Tendons, HOSAKA Yoshinao; KIRISAWA Rikio; UEDA Hiromi; YAMAGUCHI Mamoru; TAKEHANA Kazushige, Journal of Veterinary Medical Science, 67, (10) 985 - 991, 2005 , 10.1292/jvms.67.985, Tumor necrosis factor (TNF) receptors (TNF-R)-mediated cell survival or apoptosis has been demonstrated in many cells, but little is known about survival or apoptotic signals via TNF-R1 in tendinocytes. In this study, we focused on four signaling factors, TNFα, TNF-R1, TNFR-associated factor2 (TRAF2) and caspase-3, in order to elucidate the signaling events in tendinocytes. Samples were obtained from normal, inflamed and scar-formed equine superficial digital flexor tendons. To detect these signaling factors, samples were subjected to immunohistochemistry and Western blot analysis, and some samples were also subjected to reverse transcription-polymerase chain reaction (RT-PCR), PCR-Southern blot analysis and in situ hybridization to detect the expression of TNFα mRNA. Distribution of the four factors differed depending on the tendon condition, normal, inflamed or scar-formed. In the normal tendon, large amounts of TRAF2 were found in tendinocytes, but the amounts of TNF-R1 were small. TNFα mRNA was expressed most highly in the inflamed tendon. TNF-R1, which was only faintly detected in the normal tendon, was detected at a high level in the inflamed tendon, and the amounts of TRAF2 and caspase-3 also increased. Activated caspase-3 was only detected in the inflamed tendon. TNFα mRNA was also expressed in the scar-formed tendon, though it showed weak signals, and the expression levels of TNF-R1, TRAF2 and caspase-3 proteins were very low. Two distinct intracellular signaling pathways of TNFα, which lead to cell survival and apoptosis, might be present in tendinocytes mediated through TNF-R1. These results, which reflect the dynamism of TNFα, provide important clues for means to prevent tendinopathy.
ウマ浅指屈筋けんにおけるコラーゲン細線維の分岐, 渡辺敬文; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 137th, 2004 03 01 けん細胞へのデコリンアンチセンスオリゴヌクレオチド遺伝子の導入効果, 保坂善真; 桐沢力雄; 真船直樹; 山本悦子; 渡辺敬文; 尾添紗知子; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 137th, 2004 03 01 Production of decorin and TGF-bl in tendinocytes are surpressed by modificaton of decorin gene, Hosaka Y; Kirisawa R; Mafune N; Watanabe T; Ozoe S; Ueda H; Takehana K, Connective tissue, 36, (2) 104 - 104, 2004 Relationship between Serum Vitamin-A Concentration and Hepatic Stellate Cells in Cows with Fatty-liver Disease, 小川美希; 今井克幸; 及川伸; 保坂善真; 植田弘美; 竹花一成, 日本獣医師会雑誌, 57, (10) 639 - 643, 2004 , 10.12935/jvma1951.57.639 シロザケ(Oncorhynchus Keta)の体表粘液は棲息環境に応じて変化するのか, 松本紗生子; 植田弘美; 武田浩郁; 保坂善真; 竹花一成, 日本水産学会北海道支部大会講演要旨集, 2003, 2003 11 28 イヌにおける鎖骨の存在意義, 藤井崇文; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 136th, 2003 09 10 イヌの混濁を呈した角膜の形態学的および生化学的解析, 広岡雅満; 五十嵐治; 山本悦子; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 136th, 2003 09 10 ウマ浅指屈筋けんと総指伸筋けんの形態学的および生化学的性状の比較, 山本悦子; BIRCH H L; GOODSHIP A E; 渡辺敬文; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 136th, 2003 09 10 ウマ浅指屈けん炎におけるマトリックスメタロプロテアーゼ活性, 山本悦子; 渡辺敬文; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 136th, 2003 09 10 けん組織の取込み能について, 保坂善真; 山本悦子; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 136th, 2003 09 10 イヌ膝関節における前十字靭帯と外側側副靭帯の構造的差異, 松川拓也; 山本悦子; 保坂善真; 植田弘美; 中村邦男; 竹花一成, 日本獣医学会学術集会講演要旨集, 136th, 2003 09 10 ウマ浅指屈筋けんの部位差について, 瀬瀬賢; 渡辺敬文; 山本悦子; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 136th, 2003 09 10 馬浅指屈けん炎におけるマトリックスメタロプロテアーゼ活性, 山本悦子; 渡辺敬文; 保坂善真; 植田弘美; 竹花一成, 北海道獣医師会雑誌, 47, (8) 280 - 280, 2003 08 01 犬膝関節十字靭帯,側副靭帯の力学的,形態学的および生化学的相異, 松川拓也; 山本悦子; 保坂善真; 植田弘美; 中村邦男; 竹花一成, 北海道獣医師会雑誌, 47, (8) 308 - 308, 2003 08 01 犬の混濁を呈した角膜の形態学的および生化学的解析, 広岡雅満; 五十嵐治; 山本悦子; 保坂善真; 植田弘美; 竹花一成, 北海道獣医師会雑誌, 47, (8) 292 - 292, 2003 08 01 なぜウマの屈腱炎は骨腱付着部で発症しないのか?, 佐藤 紀子; 保坂 善真; 植田 弘美; 竹花 一成, 解剖学雑誌, 78, (Suppl.) 319 - 319, 2003 04 腱細胞におけるTNFα細胞内シグナル伝達因子の発現, 保坂 善真; 桐沢 力雄; 岩永 敏彦; 山本 悦子; 植田 弘美; 竹花 一成, 解剖学雑誌, 78, (Suppl.) 319 - 319, 2003 04 淡水カメへのビタミンD3投与による鰓後体と上皮小体の応答, 荒木 貴裕; 保坂 善真; 植田 弘美; 竹花 一成, 解剖学雑誌, 78, (Suppl.) 320 - 320, 2003 04 血管壁に発現する陰性荷電について, 植田 弘美; 山本 悦子; 保坂 善真; 竹花 一成, 解剖学雑誌, 78, (Suppl.) 321 - 321, 2003 04 シロザケ(Oncorhynchus keta)卵表面の塩漬に対する形態学的変化, 横澤 香菜; 保坂 善真; 植田 弘美; 武田 浩郁; 竹花 一成, 解剖学雑誌, 78, (Suppl.) 322 - 322, 2003 04 けん炎における腫よう壊死因子(TNF)αの関与, 保坂善真; 桐沢力雄; 岩永敏彦; 山本悦子; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 135th, 2003 03 25 Age-related Changes in the Morphological and Biochemical Characteristics of the Equine Superficial Digital Flexor Tendon, YAMAMOTO Etsuko; HOSAKA Yoshinao; UEDA Hiromi; NAKAMURA Fumio; TAKEHANA Kazushige, Journal of the Japan Veterinary Medical Association, 56, (2) 78 - 82, 2003 , Morphological and biochemical analyses of age-related changes in the superficial digital flexor tendon (SDFT) of thoroughbred horses showed a straightening of collagen-fibril orientation and a decrease in diameter with aging. Type III collagen content increased with aging. In terms of glycosaminoglcan composition, dermatan sulfate decreased and chondroitin sulfate increased. In the SDFT of thoroughbred horses, morphological age-related changes in collagen fibrils are accompanied by changes in biochemical composition. Uptake of erythrocyte iron by tendinocyte in inflamed tendon :, Hosaka Yoshinao; Yamamoto Etsuko; Ueda Hiromi; Takehana Kazushige, Connective tissue, 35, (2) 102 - 102, 2003 Localization of cytokines in tendinocytes of the superficial digital flexor tendon in the horse, Y Hosaka; R Kirisawa; E Yamamoto; H Ueda; H Iwai; K Takehana, JOURNAL OF VETERINARY MEDICAL SCIENCE, 64, (10) 945 - 947, 2002 10 , Although inflammatory activation of cytokines have been analyzed in various tissues, there have only been a few and as-yet-inconclusive studies on cytokines in equine tendons. In this study, the localizations of 4 cytokines (IL-1alpha, IL-1beta, TNFalpha and IFNgamma) in tendinocytes of the equine superficial digital flexor tendon (SDFT) were analyzed by the use of an immunohistochemical method. In inflamed tendons positive staining for all 4 cytokines antibodies were detected in endotedinieum cells and vascular epithelial cells. In contrast, negative or trace immunoreactions were obtained in many tendinocytes in the normal tendon. The variation in cellular immune responses depending on the kind of cytokine may reflect the physiological/pathological condition of the SDFT. 血清中ビタミンA濃度と肝臓星細胞の形態的変化について, 小川美希; 今井克幸; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 134th, 2002 08 20 新生イヌと成イヌの角膜混濁の相違性, 竹花一成; 山本悦子; 保坂善真; 植田弘美, 日本獣医学会学術集会講演要旨集, 134th, 2002 08 20 ウマ浅指屈けん炎に対するβ‐aminopropionitrile(BAPN)治療効果, 山本悦子; 保坂善真; 山下和人; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 134th, 2002 08 20 ラットのすい臓B細胞分泌に対する酢酸投与の影響, 久松和加; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 134th, 2002 08 20 けんにおける炎症性サイトカインとアポトーシス関連シグナル伝達因子の発現, 保坂善真; 桐沢力雄; 岩永敏彦; 山本悦子; 坂本由梨恵; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 134th, 2002 08 20 ウマの浅指屈けん筋におけるゴルジけん紡錘について, 渡辺敬文; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 134th, 2002 08 20 肝障害の程度と星細胞(ビタミンA貯蔵細胞)の関係, 竹花一成; 及川伸; 保坂善真; 植田弘美, 北海道獣医師会雑誌, 46, (8) 258 - 258, 2002 08 01 イヌの角膜成熟と細胞外マトリックス成分の相関性, 竹花一成; 山本悦子; 保坂善真; 植田弘美, マトリックス研究会大会, 49th, (1) 75 - 75, 2002 04 , 【目的】新生イヌの角膜は未発達な網膜や輝板を保護するために混濁しており、眼の成熟と共に透明になる.角膜の透明度は角膜固有層のコラーゲン細線維の規則正しい配列とそれらの間の細胞外マトリックスの一種であるグリコサミノグリカン(GAG)の充填が重要であるとされている.しかし,混濁とGAG構成の変化との相関性については明らかにされていない.そこでイヌの角膜の正常変化についてビーグル犬を用い形態学的および生化学的手法により解析した.【方法】形態学的には角膜のコラーゲン細線維の配列状態ならびに直径と単位面積あたりの基質占有割合を算出し,生化学的にはGAGの解析を行った.【結果】新生イヌの角膜は開眼時には混濁しており,その後徐々に混濁は和らぎ,生後3週目前後で完全に透明になった.角膜の主体である角膜固有層のコラーゲン細線維の直径は週齢毎でほぼ均一で0週齢では24.0nm程度,成イヌでは38.0nmであった.コラーゲン細線維の配列は成熟に伴い規則性を有していた.基質占有割合は80%から30%と減少していた.基質のGAG組成は週齢の増加と共にケラタン硫酸(KS)が増加しデルマタン硫酸(DS)が減少していた.【考察】角膜成熟に伴いコラーゲン細線維の太さと規則性の変化が認められ、生化学的には、KSの増加とDSの減少が顕著であった。このことは、コラーゲン細線維に対するKSとDSの作用、すなわちその直径と空間の維持と形成抑制作用に由来するもので、角膜の成熟にはこれらの成分の存在は必要不可欠であることが明らかになった。 けん成熟に対するMMPの作用, 山本悦子; 保坂善真; 植田弘美; 竹花一成, マトリックス研究会大会, 49th, (1) 88 - 88, 2002 04 , 【目的】浅指屈筋腱は腱細胞、コラーゲン細線維と非コラーゲン性マトリックスにより構成されている。ウマの腱における細胞外マトリックスの加齢性変化の研究は、コラーゲンについては多数報告されているが、マトリックス分解系についての報告はほとんどない。そこで、コラーゲンの細胞外分傍系の1つであるマトリツクスメタロブロテアーゼ(MMP)に著目し、腱の加齢性変化とMMPの関与を検索した。【材料および方法】材料は、サラブレッド種(0歳5例、1歳2例、2歳2例、3歳1例、計10例)の浅指屈筋腱中手骨中央部を用いた。形態学的には、電子顕微鏡を用いてコラーゲン細線維の走行と直径の変化を視察した。生化学的には、二次元電気泳動法にてグリコサミノグリカン(GAG)の分離・同定を行った。さらに、Film in situ ZymographyとザイモグラフィーにてMMPの分離・同定を行った。【結果および考察】コラーゲン細線維は0歳では波状に走行しており、3歳では長軸に沿って平行に走行していた。細線維直径は0歳では20nmと100nmが多く、加齢に伴ってそれぞれ60nm、220nmと太くなり、その割合は加齢に伴い細いものが増加した。GAGはヒアルロン酸、コンドロイチン硫酸、デルマタン硫酸で構成されており、加齢に伴いデルマタン硫酸が減少し、コンドロイチン硫酸が増加した。MMPはMMP-2とMMP-9の潜在型と活性型が存在し、加齢に伴いMMP-9が流少し、MMP-2が増加した。以上のことより、腱コラーゲン細線維の加齢に伴う形態変化は、コラーゲン細線維形成調節作用を持つGAGによって起こり、分解系であるMMPも作用していることが示唆された。 ウマ腱細胞におけるアポトーシス発現とサイトカインの関与について, 保坂 善真; 山本 悦子; 植田 弘美; 竹花 一成, 解剖学雑誌, 77, (Suppl.) APPENDIX24 - APPENDIX24, 2002 03 Ultrastructure and function of sertoli cell processes in the Korean native goat, Geon Oh Kwon; Young Seok Park; Seong Ho Lee; Etsuko Yamamoto; Yoshinao Hosaka; Hiromi Ueda; Kazushige Takehana, Okajimas Folia Anatomica Japonica, 79, (4) 121 - 128, 2002 , 10.2535/ofaj.79.121, The infrastructure of Sertoli cell in the testes of Korean native goats was investigated by scanning and transmission electron microscopy to elucidate the relationship between germ cell and Sertoli cell processes in the spermiogenetic cycle. Type-A Sertoli cells at stage V of the spermiogenetic cycle and type-B Sertoli cells at stage Vu of the spermiogenetic cycle were used for analysis. Morphologically, the Sertoli cell processes were classified into sheet-like and slender cord-like processes. The sheet-like process originated solely from the Sertoli cell column while the slender cord-like process projected either from the Sertoli cell column or the sheet-like process. Periodic acid (PA)-thiocarbohydrazide (TCH)-silver protein (SP)-physical development (PD)-positive granules were found diffusively both in the sheet-like and slender cord-like processes near the round spermatid, whereas they had accumulated near the head of the elongated spermatid. The morphological variation and glucoconjugate histochemical reaction of the Sertoli cell processes reflect nourishment, movement and transformation of the spermatogenic cells in accordance with spermiogenesis. RELATION BETWEEN INFLAMMATORY CYTOKINES AND APOTOSIS EXPRESSION IN TENDON : AN ANALYSIS IN THE EQUINE SUPERHCIAL DIGITAL FLEXOR TENDON, Hosaka Yoshinao; Yamamoto Etsuko; Ueda Hiromi; Takehana Kazushige, Connective tissue, 34, (1) 86 - 86, 2002 , 【目的】アポトーシスは多くの組織で発現報告がなされているが、腱組織では明らかにされていない。本研究では腱組織での腱細胞のアポトーシス発現の有無を検索するとともに、正常時および炎症時での発現の差異を明らかにすることを目的とした。加えて、腱組織内での炎症性サイトカイン陽性細胞の分布を検索し、アポトーシス発現との関与を明らかにした。【材料と方法】ウマの浅指屈筋腱中央部を試料として用いた。試料は肉眼的に異常が全く認められなかったものを正常腱とし、出血・腫脹等の炎症像が見られたものを炎症腱とした。アポトーシス発現の検索はTUNEL法と抗Caspase3抗体による免疫組織化学的手法で、サイトカイン陽性細胞の分布は免疫組織化学的手法およびin situ hybridization法で検索した。形態変化については透過型電子顕微鏡で観察した。【結果と考察】正常腱、炎症腱ともにTUNEL陽性細胞およびCaspase3陽性細胞が確認された。正常腱では陽性細胞が散発的に分布していたのに対し、炎症腱では正常腱に比して多数の陽性細胞が分布していた。電顕でも核クロマチンの凝集像を持つ腱細胞が炎症腱で多く認められ、腱組織中でのアポトーシス発現が明らかになった。サイトカイン陽性細胞は正常腱では散発的に分布していたが、炎症腱では組織内に遊走したマクロファージ等の免疫細胞に加え、腱(芽)細胞、腱内膜細胞および血管内皮細胞が張陽性を呈した。腱炎時に腱組織の細胞外マトリックス構成成分が変化することはすでに明らかにされている。炎症による健内の環境変化が腱細胞のサイトカイン産生を促進し、産生されたサイトカインがさらに腱細胞のアポトーシスの誘発に関与していると推察した。 Liver fattening in cattle presenting with ketosis and its correlation with basal membrane inferior to sinusoid endothelial cell., 岸基史; 東伸好; 保坂善真; 植田弘美; 及川伸; 小岩政照; 竹花一成, 家畜診療, 49, (474) 771 - 776, 2002 Micromorphological characteristics of hepatic sinusoidal endothelial cells and their basal laminae in five different animal species, Nobuyo Higashi; Hiromi Ueda; Osamu Yamada; Shin Oikawa; Masateru Koiwa; Prasarn Tangkawattana; Kazushige Takehana, Okajimas Folia Anatomica Japonica, 79, (5) 135 - 142, 2002 , 10.2535/ofaj.79.135, The micromorphological characteristics of hepatic sinusoidal endothelial cells and their basal laminae in five different animal species (cow, sheep, guinea pig, pig, and dog) were studied by electron microscopy. Using transmission electron microscopy, a prominent basal lamina was seen only in cow and sheep. In pig and guinea pig, no prominent basal lamina was evident. Basal lamina-like material was occasionally found in dog. Immunohistochemically, basal lamina was found in cow, sheep, and dog. Using scanning electron microscopy, the size of endothelial fenestrae differed between species. These results may suggest that variation of endothelia and their basal laminae of different species is related to differences in nourishment. ウシ十二指腸における生後の粘膜変化について, 渡辺洋輔; 山本悦子; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 132nd, 2001 09 07 ウマ浅指屈筋けんにおけるアポトーシス発現, 保坂善真; 山本悦子; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 132nd, 2001 09 07 ウマ浅指屈筋けんの加齢性変化 III コラーゲン細線維とGAG,MMPの関連性について, 山本悦子; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 132nd, 2001 09 07 イヌ子宮畜膿症における子宮内膜の形態学的および生化学的性状, 有泉幸雄; 山本悦子; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 132nd, 2001 09 07 新生イヌと成イヌの角膜混濁の相違性, 竹花一成; 山本悦子; 保坂善真; 植田弘美, 北海道獣医師会雑誌, 45, (8) 287 - 287, 2001 08 01 犬の経穴の構造について, 大田晃之; 山本悦子; 横沢香菜; 保坂善真; 植田弘美; 竹花一成, 北海道獣医師会雑誌, 45, (8) 285 - 285, 2001 08 01 イヌの鼻涙管の走行について, 源本泰士; 山本悦子; 保坂善真; 植田弘美; 竹花一成, 北海道獣医師会雑誌, 45, (8) 286 - 286, 2001 08 01 Electron microscopic cytochemical studies of anionic sites in the rat spleen, H Ueda; K Takehana; Eerdunchaolu; K Iwasa; O Fujimori; S Shimada, JOURNAL OF VETERINARY MEDICAL SCIENCE, 63, (3) 287 - 291, 2001 03 , 10.1292/jvms.63.287, The distribution patterns of the intensity of negative charge on the free surfaces (glycocalyx of the plasma membrane) of endothelial cells (ECs) in blood vessels and reticular cells (RCs) in the splenic cord of the rat spleen were studied by an electron microscopic cytochemical method using polyethyleneimine (PEI) as a cationic probe. Spleens from adult male rats were perfusion-fixed with 0.5% glutaraldehyde - 4 % paraformaldehyde containing 0.05% cetylpyridinium chloride and then perfused with 0.58 PEI at pH 7.4. On the free surfaces (glycocalyx of the plasma membrane) of the ECs examined, distinct PEI-positive reactions were observed in blood vessels, such as trabecular :arteries, central arteries, arterial capillaries, pulp veins and trabecular veins. These PEI-positive electron-dense substances in the trabecular arteries, central arteries, and trabecular veins took the shape of a band of 170-250 nm in thickness. On the other hand, the corresponding ultrastructure of the ECs lining the splenic sinuses and the RCs in the splenic cord showed exceedingly weak PEI reactions. The PEI-reactive deposits were significantly thinner than those in the above blood vessels. As the thickness of the electron-dense substances can be related to the density of the negative charge, these results suggest that there is a high intensity of negative charge on the free surfaces (glycocalyx of the plasma membrane) of ECs in blood vessels where blood cells and plasma pass into the red pulp ol are discharged from the red pulp. In contrast, the splenic sinuses and RCs, which are the main components of the red pulp, contain weakly negative-charged sites. This may contribute to the microcirculation of the splenic blood vessels and elucidate the possible physiological functions of the spleen, such as blood storage. A histochemical study of the camel (Camelus bactrianus) duodenal glands, K Takehana; Eerdunchaoluo; H Ueda; A Kobayashi; K Iwasa; K Sou, JOURNAL OF VETERINARY MEDICAL SCIENCE, 62, (4) 449 - 452, 2000 04 , 10.1292/jvms.62.449, The complex carbohydrates in the camel duodenal glands were examined histochemically at light and electron microscopic levels. The duodenal glands of the camel were distributed in the submucosa 2 m caudal from the pylorus. These were branched tubuloalveolar glands. The terminal portion of each lobule was formed by only one type of mucous cell. The duodenal gland cells contained acidic and neutral carbohydrates. The mucous cells mainly contained sulfate and carboxyl carbohydrate with sialic acid, and they also contained a few neutral carbohydrates with different saccharide residues such as mannose, glucose, galactose, N-acetyl glucosamine and N-acetyl galactosamine. The results showed that the secretary granules of the duodenal glands in the camel contain mainly acidic carbohydrates. These findings seem to be the morphological characteristics of the duodenal glands in the camel. Secretary Process Examined Morphologically in B-cell Granules of the Rat Pancreas Islet, OKADA Akira; TAKEHANA Kazushige; EERDUNCHAOLU; KOBAYASHI Ataru; UEDA Hiromi; IWASA Kenji, Journal of the College of Dairying. Natural science, 24, (1) 55 - 62, 1999 Secretary process examined morphologically in B-cell granules of the rat pancreas islet., OKADA Akira; TAKEHANA Kazushige; EERDUNCHAOLU; KOBAYASHI Ataru; UEDA Hiromi; IWASA Kenji, Journal of Rakuno Gakuen University, 24, (1) 55 - 62, 1999 Effects of a triple enzyme digestion method on a diamine reaction for glycosaminoglycans of the at aorta in electron microscopy, H Ueda; O Fujimori, JOURNAL OF VETERINARY MEDICAL SCIENCE, 60, (11) 1169 - 1174, 1998 11 , 10.1292/jvms.60.1169, Effects of a triple enzyme digestion method using glycosaminoglycans-degrading enzymes upon a diamine reaction have been tested for electron microscopic histochemical detection of glycosaminoglycans in extracellular matrix of the rat aorta. The triple enzyme digestion method consists of a sequence of chondroitinase B, testicular hyaluronidase and heparitinase. The results obtained by the present experimental and control studies indicated that dermatan sulfates, chondroitin sulfates (A and/or C) or heparan sulfates were apparently observed in various ultrastructural features of aortic extracellular matrix, such as bundle of collagen fibers and soluble matrix of interstitial space. Particularly, we found that both heparan sulfates and chondroitin sulfates (A and/or C) were detected in association with the basal lamina of smooth muscle cells and the external surface of elastic lamina, and in the latter heparan sulfates were frequently recognized as a mass, whereas chondroitin sulfates (A and/or C) were found intermittently along the external surface of elastic lamina. This suggests that the triple enzyme digestion method which combines the glycosaminoglycans-degrading enzymes with the diamine reaction can be postulated to represent efficient and useful technique for precise electron microscopic histochemical detection of the glycosaminoglycans in the extracellular matrix of the rat aorta. Cytochemical studies on carbohydrates in the reticular cells of the rat splenic cord, H Ueda; O Fujimori, ACTA HISTOCHEMICA ET CYTOCHEMICA, 31, (1) 77 - 79, 1998 , 10.1267/ahc.31.77, Carbohydrates in the reticular cells of the rat splenic cord were studied by means of a series of electron microscopic cytochemical methods. The results indicated that the reticular cells contained an appreciable abundance of glycogen particles in the cytoplasm. In the free surface coat of the plasma membrane, the cells included neutral glycoprotein, which were devoid of detectable amounts of acidic glycoconjugates. The possible physiological significance of these carbohydrates is discussed, with special reference to their characteristic cytochemical features examined in the present study. Histochemical analysis of acidic glycoconjugates in the endothelium lining the splenic blood vessels in the rat, H Ueda; O Fujimori; M Abe, ARCHIVES OF HISTOLOGY AND CYTOLOGY, 59, (4) 389 - 397, 1996 10 , 10.1679/aohc.59.389, An electron microscopic analysis with specific histochemical stainings for acidic glycoconjugates was carried out to examine the endothelium lining blood vessels of the rat spleen, Histochemical staining performed was the postembedding high or low iron diamine-thiocarbohydrazide-silver protein-physical development (HID or LID-TCH-SP-PD) method, with or without prior digestion with acidic glycoconjugate-degrading enzymes, such as heparitinase, testicular hyaluronidase, chondroitinase B and neuraminidase. The results indicated that the acidic glycoconjugates in the basal lamina of the endothelial cells lining the four types of blood vessel(central arteries, arterial capillaries, splenic sinuses and pulp veins) were heparan sulfate, chondroitin sulfate A, and/or C, chondroitin sulfate B and sialic acid residues. In the endothelial cells lining the central arteries, arterial capillaries and pulp veins, the surface coat of the luminal plasma membrane included heparan sulfate, chondroitin sulfate A and/or C, chondroitin sulfate B and sialic acid residues, whereas the corresponding ultrastructure of the splenic sinuses was devoid of detectable amounts of acidic glycoconjugates. This suggests that such characteristic histochemical features of the endothelium in the four types of the splenic blood vessel can be related to the possible physiological functions of the spleen. Electron Microscopic Histochemical Studies on Carbohydrates in the Endothelium Lining the Splenic Blood Vessels of the Guinea Pig, HATTORI OSAMU; UEDA HIROMI, The Journal of the Nagoya City University Medical Association, 47, (1) 31 - 54, 1996 02 01 CARBOHYDRATES IN THE EPIDERMAL MUCOUS CELLS OF A FRESH-WATER FISH MASTACEMBELUS-PANCALUS (MASTACEMBELIDAE, PISCES) AS STUDIED BY ELECTRON-MICROSCOPIC CYTOCHEMICAL METHODS, AK MITTAL; O FUJIMORI; H UEDA; K YAMADA, CELL AND TISSUE RESEARCH, 280, (3) 531 - 539, 1995 06 , Carbohydrates in the mucous cells of the epidermis of the fish Mastacembelus pancalus were studied by means of electron-microscopic cytochemical methods using physical development procedures. Three types of mucous cells (types A-C) were differentiated on the basis of the reactivities of the secretory products elaborated by them. The carbohydrate contents of mucous globules predominantly comprised sulfate esters and traces of oxidizable vicinal diols in type-A cells, oxidizable vicinal diols in type-B cells, and moderate amounts of both sulfate esters and oxidizable vicinal diols in type-C cells. Glycogen particles were also found to occur in the cytoplasm of these cells and glycoproteins containing oxidizable vicinal diols were visualized in Golgi cisternae, rough endoplasmic reticulum, nuclear envelopes, and plasma membranes. In the type-A and type-B cells situated in the superficial layers of the epidermis, extensive cisternae of the Golgi apparatus and copious rough endoplasmic reticulum suggested the active syntheses of secretory contents, in contrast to the type-C mucous cells, which displayed poor development of these organelles, in the deeper layers. A POSTEMBEDDING METHOD USING UREA-UNMASKED ULTRATHIN SECTIONS FOR THE ELECTRON-MICROSCOPIC DETECTION OF SULFATED GLYCOCONJUGATES, H UEDA; O FUJIMORI; K YAMADA, ACTA HISTOCHEMICA ET CYTOCHEMICA, 26, (1) 27 - 34, 1993 , 10.1267/ahc.26.27, Using urea-unmasked ultrathin sections, a postembedding staining method in combination with nitrous acid procedure has been established for the efficient visualization of sulfated glycoconjugates in a series of ultrastructures of three salivary glands of the rat. Pieces of the parotid, submandibular and sublingual glands from adult rats were subjected to glutaraldehyde-paraformaldehyde fixation, rinsed, dehydrated and embedded in LR-White resin. Ultrathin sections were prepared, grid-mounted, incubated in urea (6M) aqueous solution, and then stained with an alcian blue (AB) pH 1.0-phosphotungstic acid (PTA) sequence with or without prior treatment with nitrous acid. The results obtained have shown that sulfated glycoconjugates are involved in such ultrastructures as mast cell granules, basal lamina of arteriolar endothelial cells and intercellular matrix of arteriolar smooth muscle cells. CYTOPLASMIC FILAMENTS IN THE ENDOTHELIAL-CELLS OF THE SHEATHED CAPILLARY - AN ULTRASTRUCTURAL AND IMMUNOCYTOCHEMICAL STUDY IN THE PIG SPLEEN, K TAKEHANA; M ABE; M YAMAGUCHI; T UCHIDA; M INAGAKI; K YAMAMOTO; J MASTY; A WINNARD; H UEDA; H MIYATA, ACTA ANATOMICA, 143, (4) 294 - 300, 1992 04 , Cytoplasmic filaments of the endothelial cells of sheathed capillaries in the pig spleen were identified and their ultrastructure was studied. Two types of cytoplasmic filaments were found: intermediate filaments (diameter: 10 nm) which filled most of the interior of the cells, and thin filaments (diameter: 5 nm) which were located just beneath the cell membrane and filled the lateral cytoplasmic processes. In immunocytochemical preparations, the intermediate filaments were positive for vimentin and desmin, and were negative for keratin. Staining of the thin filaments with heavy meromyosin resulted in arrowhead formations. These observations suggest that the intermediate filaments maintain the cytoarchitecture, possibly protecting the cell from structural alterations induced by blood pressure changes. Concurrently, thin filaments may facilitate the passage of red blood cells and blood platelets through the interendothelial fenestrae of the sheathed endothelial cell to the reticular meshwork in the capillary sheath. ULTRASTRUCTURE OF THE RED PULP IN SPLEEN INNERVATION IN HORSE AND PIG, H UEDA; M ABE; K TAKEHANA; K IWASA; T HIRAGA, ACTA ANATOMICA, 141, (2) 151 - 158, 1991 06 , The innervation of the red pulp in the spleen of horse and pig was investigated by electron microscopy. In addition, the neurilemma was studied by immunohistochemistry specific for S-100 protein. In the pig, a large population of smooth-muscle cells extending from the smooth-muscle trabeculae was present in the red pulp. The cytoplasmic processes of reticular cells enwrapped the smooth-muscle cells, and nerve fibres were distributed between the smooth-muscle cells and the reticular cells. The nerve terminals clustered toward the facing of the smooth-muscle cells. Nerve fibres and terminals were not disclosed within the sheathed artery. Immunohistochemically the neurilemma showed a reaction positive for S-100 protein. In the horse, no smooth-muscle cells were noted in the red pulp. The nerve fibres terminated around the cytoplasmic processes of the reticular cells. Nerve fibres and terminals were disclosed within the sheathed artery, and the terminals contained both large and small dense-cored vesicles. Immunohistochemically the neurilemma showed a reaction negative for S-100 protein. These findings support the presence of the axon-bearing reticular cells described earlier in the horse spleen. A HISTOCHEMICAL METHOD FOR THE DEMONSTRATION OF UNSATURATED LIPIDS, O FUJIMORI; H UEDA; K YAMADA, HISTOCHEMICAL JOURNAL, 23, (2) 91 - 99, 1991 02 , A method has been developed for the histochemical demonstration of unsaturated lipids in light microscopy. It is a peracetic acid-thiocarbohydrazide-silver protein sequence followed by a physical development procedure. In the present study on paraffin and cryostat sections of liver, brain and ovary, unsaturated lipids were visualized as distinct reaction products coloured various shades of brown and black. The reaction products are easier to see and the method is more efficient than the peracetic acid-Schiff method. Cytochemical studies on vicinal diol-containing carbohydrates in the endothelium of the rat splenic blood vessels, Ueda, H; Yanase, Y; Fujimoro, O; Yamada, K, Nagoya Medical Journal, 36, (2) 115 - 127, 1991 AN EFFICIENT HISTOCHEMICAL METHOD FOR UNSATURATED LIPIDS USING A SILVER ENHANCEMENT PROCEDURE, O FUJIMORI; H UEDA; K YAMADA, JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 38, (7) 1060 - 1060, 1990 07 ULTRASTRUCTURE OF SPLEEN INNERVATION IN THE HORSE, COW, PIG AND CHICKEN, H UEDA; M ABE; K TAKEHANA; K IWASA; T HIRAGA; N KUDO, JOURNAL OF ELECTRON MICROSCOPY, 39, (2) 129 - 129, 1990 04 Electron microscopic cytochemistry of neutral carbohydrates in the endothelial cells lining the splenic blood vessels of the rat. :, Ueda H.; Yanase Y.; Yamada K., Acta histochemica et cytochemica, 23, (5) 745 - 745, 1990 ELECTRON-MICROSCOPIC STUDIES OF THE INNERVATION AND CELLS ENFOLDING AXONS IN THE HORSE, PIG AND CHICKEN SPLEENS, M ABE; H UEDA; K TAKEHANA; K IWASA; T HIRAGA, JOURNAL OF ELECTRON MICROSCOPY, 38, (4) 277 - 277, 1989 MORPHOLOGY OF THE ENDOTHELIAL-CELL OF THE SHEATHED ARTERY IN THE SPLEEN, H UEDA; M ABE; K TAKEHANA; K IWASA; T HIRAGA, JOURNAL OF ELECTRON MICROSCOPY, 37, (5) 254 - 255, 1988 A COMPARATIVE-STUDY OF THE SHEATHED ARTERY OF HORSE, COW, PIG, DOG, MINK AND CHICKEN SPLEENS, M ABE; H UEDA; K TAKEHANA; K IWASA; T HIRAGA, JOURNAL OF ELECTRON MICROSCOPY, 37, (2) 108 - 108, 1988
Awards & Honors
2021 09 , 日本獣医解剖学会, 日本獣医解剖学会奨励賞, ニワトリ速筋線維の各サブタイプを構成するミトコンドリアの三次元構造解析 2019 12 , 公益財団法人日本顕微鏡学会北海道支部, 令和元年日本顕微鏡学会北海道支部学術講演会北海道支部長賞, ドライエイジングによる筋組織の形態変化の解析
Research Grants & Projects
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Cloning and functional analysis of taste receptors for sourness and saltiness, 1998, 2000, SHIMADA Shoichi; UEDA Takashi; HIRABAYASHI Yoshifumi; FUJIMORI Osamu; UGAWA Shinya, We have identifieda cDNA encoding a taste receptor for sourness (MDEG1) from a rat circumvallate papilla cDNA library by combined cDNA homology and functional expression approaches. When expressed in Xenopus laevis oocytes, MDEG1 showed characteristics of a proton-gated amiloride-sensitive cation channel. Interestingly, acidic stimulation by acetic acid, the chief ingredient of vinegar, generatedlarger inwardcurrents than that by hydrochloric acidat equal pH.In situ hybridization revealed that the mRNA of MDEG1 was exclusively localized in taste buds. MDEG1 immunoreactivity was found on the cell surface and in the apical portion of taste bud cells, especially in type III cells which are classifiedas gustatory receptor cells. This is the first taste receptor cDNA to be cloned. To isolate another subunit of the sour-taste receptor, we screened a rat circumvallate papilla cDNA library and iden tified MDEG2. Immunohistochemical and electrophysiological studies demonstrated that MDEG2 formed heterooligomeric channels with MDIEG1 at the apical portion of single taste-cells and modified the desensitization of MDEG1 channel. The desensitization rate of the heterooligomer was dramatically altered by a single amino acid substitution of Gly481 in MDEG2 without changing other channel properties. These findings suggest that MDEG2 regulates the sour-taste receptor desensitization contributing to sour-taste adaptation and that Gly481 in MDEG2 is the key amino acidresidue that controls the duration of sourtaste sensation. 脾臓微細血管系の陰性荷電に関する研究 脾臓微細血管系の複合糖質に関する研究 Study on negative charge in splenic blood vessels Study on carbohydrates in splenic blood vessels
社会貢献活動情報
Social Contribution
2021 04 01 - 2023 03 31 , 公益社団法人日本顕微鏡学会北海道支部支部長# 2019 09 07 - 2019 09 08 , 日本解剖学会第65回東北・北海道連合支部学術集会, 日本解剖学会北海道支部, 日本解剖学会第65回東北・北海道連合支部学術集会を二日間に渡って酪農学園大学の学生ホールにて開催し、私は大会長を務めた。# 2019 08 24 , 市立札幌開成中等教育学校での先端科学特論における講師, 市立札幌開成中等教育学校, 先端科学特論# 2018 10 24 , 動物愛護教室での講師, 北海道宗谷総合振興局長, 稚内市立東小学校での動物愛護教室# 2018 08 21 , 生物教育分科会研修での講師, 広島市食肉衛生検査所, 広島県私立中学・高等学校生物教育研究会# 2018 08 21 , 体験実習での講師, 広島市食肉衛生検査所, 夏休み親子体験教室# 2017 08 26 , 市立札幌開成中等教育学校での先端科学特論における講師, 市立札幌開成中等教育学校, 先端科学特論# 2016 08 27 , 市立札幌開成中等教育学校での先端科学特論における講師, 市立札幌開成中等教育学校, 先端科学特論#
教員総覧
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Last Update :2024/12/18
Researcher Profile and Settings
Name
| UEDA Hiromi |
Affiliation (Master)
| School of Veterinary Medicine |
Affiliation & Job
| Rakuno Gakuen University |
| School of Veterinary Medicine, Department of Veterinary Medicine |
Degree
| Veterinary Medical Doctor, Rakuno Gakuen University |
| Veterinary Medical Master, Rakuno Gakuen University |
Association Memberships
| JAPAN VETERINARY MEDICAL ASSOCIATION |
| THE JAPANESE SOCIETY OF MICROSCOPY |
| 日本組織細胞化学会 |
| 日本解剖学会 |
| 日本獣医学会 |
Research Activities
Research Areas
| Life sciences, Anatomy |
| Life sciences, Veterinary medicine |
Research Interests
| herbal medicine, |
| fatty liver, |
| liver, |
| diabetes, |
| histology, |
Published Papers
| Morphological and biochemical characterization of Holstein cow skin at the tail root region susceptible to <i>Chorioptes bovis</i> and <i>texanus</i> parasitism, Emi FUJII; Hotaka HASHIMOTO; Shin-ichiro FUKUMOTO; Naoyuki MAEDA; Yasuhiro HASEGAWA; Tomohito IWASAKI; Marina HOSOTANI; Hiromi UEDA; Takafumi WATANABE, Journal of Veterinary Medical Science, Japanese Society of Veterinary Science, 2024 |
| Oviduct Histopathology of Internal Laying and Egg-Bound Syndrome in Laying Hens, Marina Hosotani; Sohei Hamano; Tomohito Iwasaki; Yasuhiro Hasegawa; Hiromi Ueda; Takafumi Watanabe, Veterinary Sciences, {MDPI} {AG}, 10, (4) 260 - 260, 2023 03 29 |
| The histopathological features of the surgical endometriosis model using systemic autoimmune disease-prone mice., Marina Hosotani; Machiko Akita; Hiromi Ueda; Takafumi Watanabe, The Journal of veterinary medical science, 85, (1) 1 - 8, 2022 11 28 |
| Morphological analysis of cell distribution and network structure via gap junctions in swine corneal stroma., Nobutaka Hoshino; Kazushige Takehana; Marina Hosotani; Kiyokazu Kametani; Tomohito Iwasaki; Yasuhiro Hasegawa; Hiromi Ueda; Takafumi Watanabe, The Journal of veterinary medical science, 84, (12) 1621 - 1632, 2022 10 24 |
| 採卵鶏における卵管形態に着目した産卵障害の病態解析, 浜野 爽平; 渡邉 敬文; 岩崎 智仁; 長谷川 靖洋; 植田 弘美; 細谷 実里奈, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 165回, [A3P - 15], 2022 09 |
| Comparing chemiluminescent response and intracellular bactericidal activity of bovine blood neutrophils and monocytes against Salmonella Dublin and Escherichia coli, Satoh Hideyasu; Gondaira Satoshi; Higuchi Hidetoshi; Ueda Hiromi; Nohara Masakatsu; Nagahata Hajime, Japanese Journal of Veterinary Research, 70, (2) 71 - 77, 2022 05 |
| Anatomical features of ossa cordis in the Steller sea lion., Miki Yoshida; Kenjiro Miyoshi; Tomohiko Tajima; Akihiko Wada; Hiromi Ueda; Takanori Kooriyama, The Journal of veterinary medical science, 84(5), (1) 660 - 665, 2022 05 |
| Three-dimensional structural analysis of mitochondria composing each subtype of fast-twitch muscle fibers in chicken., Sachi Makida; Kiyokazu Kametani; Marina Hosotani; Naoki Takahashi; Tomohito Iwasaki; Yasuhiro Hasegawa; Tomohide Takaya; Hiromi Ueda; Takafumi Watanabe, The Journal of veterinary medical science, 84, (6) 809 - 816, 2022 04 14 |
| マウス卵管線毛上皮細胞と排卵卵子の相互作用 加齢と免疫異常は卵子輸送速度を変化させる, 細谷 実里奈; 市居 修; 渡邉 敬文; 植田 弘美; 昆 泰寛, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 164回, [AO - 37], 2021 09 |
| Enhancement Effect of Hepatocellular Mitochondrial Activity in Diabetic Rats with Orally Administered Kampo Medicine, Hiromi Ueda; Yuta Ito; Tomoko Nozaki; Naoki Takahashi; Marina Hosotani; Takafumi Watanabe, The journal of comparative integrative medicine/ Japan, 27, (2) 5 - 12, 2021 03 |
| Unique histological features of the tail skin of cotton rat (Sigmodon hispidus) related to caudal autotomy., Marina Hosotani; Teppei Nakamura; Osamu Ichii; Takao Irie; Yuji Sunden; Yaser Hosny Ali Elewa; Takafumi Watanabe; Hiromi Ueda; Takashi Mishima; Yasuhiro Kon, Biology open, 10, (2) , 2021 02 19 |
| Three‐dimensional ultrastructure reconstruction of tendinous components at the bifurcation of the bovine superficial digital flexor tendon using array and STEM tomographies, Naoki Takahashi; Kiyokazu Kametani; Ryo Ota; Prasarn Tangkawattana; Tomohito Iwasaki; Yasuhiro Hasegawa; Hiromi Ueda; Marina Hosotani; Takafumi Watanabe, Journal of Anatomy, Wiley, 238, (1) 63 - 72, 2021 01 |
| Anatomy and histology of the foramen of ovarian bursa opening to the peritoneal cavity and its changes in autoimmune disease‐prone mice, Marina Hosotani; Osamu Ichii; Teppei Nakamura; Takashi Namba; Md. Rashedul Islam; Yaser Hosny Ali Elewa; Takafumi Watanabe; Hiromi Ueda; Yasuhiro Kon, Journal of Anatomy, Wiley, 238, (1) 73 - 85, 2021 01 |
| Physiological and Pathological Mitochondrial Clearance Is Related to Pectoralis Major Muscle Pathogenesis in Broilers With Wooden Breast Syndrome, Marina Hosotani; Takeshi Kawasaki; Yasuhiro Hasegawa; Yui Wakasa; Maki Hoshino; Naoki Takahashi; Hiromi Ueda; Tomohide Takaya; Tomohito Iwasaki; Takafumi Watanabe, Frontiers in Physiology, Frontiers Media SA, 11, 579 - 579, 2020 06 16 |
| Ultrastructural study of the three-dimensional tenocyte network in newly hatched chick Achilles tendons using serial block face-scanning electron microscopy., Shu Hadate; Naoki Takahashi; Kiyokazu Kametani; Tomohito Iwasaki; Yasuhiro Hasega; Prasarn Tangkawattana; Takeshi Kawasaki; Hiromi Ueda; Marina Hosotani; Takafumi Watanabe, The Journal of veterinary medical science, 82, (7) 948 - 954, 2020 05 15 |
| 3次元電子顕微鏡を用いたアキレス腱の腱細胞の細胞質突起の立体構造解析, 羽立 洲; 高橋 直紀; 細谷 実里奈; 植田 弘美; 渡邉 敬文, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 162回, 334 - 334, 2019 08 |
| ウシ浅趾屈筋腱分岐部のコラーゲン細線維の走行と超微細構造の3次元解析, 高橋 直紀; 亀谷 清和; 大多 亮; 細谷 実里奈; 植田 弘美; 渡邉 敬文, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 162回, 334 - 334, 2019 08 |
| Fibrillar architecture at three different sites of the bovine superficial digital flexor tendon, Naoki Takahashi; Takuya Hirose; Jun A. Minaguchi; Hiromi Ueda; Prasarn Tangkawattana; Kazushige Takehana, Journal of Veterinary Medical Science, Japanese Society of Veterinary Science, 80, (3) 405 - 412, 2018 |
| Natural larval Echinococcus multilocularis infection in a Norway rat, Rattus norvegicus, captured indoors in Hokkaido, Japan, Shin-ichiro Fukumoto; Seitaro Yamada; Manato Fushikida; Shotaro Toyada; Tomotaka Nishikawa; Hidetoshi Higuchi; Hiroshi Ueno; Hiromi Ueda; Hiromu Sugiyama; Yasuyuki Morishima, JOURNAL OF VETERINARY MEDICAL SCIENCE, JAPAN SOC VET SCI, 79, (11) 1857 - 1860, 2017 11 |
| Remodeling of rat sir stormal-vascular cells to brite/beige adipocytes by prolyl-hydroxyproline, Jun A. Minaguchi; Sakino Ogata; Naoki Takahashi; Takuya Hirose; Hiromi Ueda; Kazushige Takehana, JOURNAL OF VETERINARY MEDICAL SCIENCE, JAPAN SOC VET SCI, 79, (3) 547 - 553, 2017 03 |
| Anti-diabetic activities of traditional Chinese herbal medicine in streptozotocin-induced diabetic rats, Tomoko Nozaki; Jun Minaguchi; Kazushige Takehana; Hiromi Ueda, Okajimas Folia Anatomica Japonica, Okajima Foria Anatomica Yaponika Henshubu, 93, (4) 111 - 118, 2017 02 01 |
| Anti-diabetic activities of traditional Chinese herbal medicine in streptozotocin-induced diabetic rats, Tomoko Nozaki; Jun Minaguchi; Kazushige Takehana; Hiromi Ueda, Okajima Folia Anatomia Japonica, 79, 574 - 553, 2017 |
| Morphometric analysis of growing tenocytes in the superficial digital flexor tendon of piglets, Naoki Takahashi; Prasarn Tangkawattana; Yoshiki Ootomo; Takuya Hirose; Jun Minaguchi; Hiromi Ueda; Michi Yamada; Kazushige Takehana, Journal of Veterinary Medical Science, Japanese Society of Veterinary Science, 79, (12) 1960 - 1967, 2017 |
| Effectiveness of Isoprothiolane in Preventing Fatty Liver in Dairy Cows, Minaguchi, J; Miura, M; Oikawa, S; Handa, F; Haraguchi, T; Miyamoto-Ohno, K; Ito, Y; Hosaka, Y; Ueda, H; Takehana, K, The Open Veterinary Science Journal, 7, 7 - 11, 2013 01 |
| Histological differences of skin among three body regions in male and female Hokkaido sika deer (Cervus nippon yesoensis), Jun A. Minaguchi; Ayako Abe; Hiromi Ueda; Prasarn Tangkawattana; Kazushige Takehana, Okajimas Folia Anatomica Japonica, 89, (1) 1 - 5, 2012 05 |
| Concerted and adaptive alignment of decorin dermatan sulfate filaments in the graded organization of collagen fibrils in the equine superficial digital flexor tendon., Takafumi Watanabe; Yasutada Imamura; Daisuke Suzuki; Yoshinao Hosaka; Hiromi Ueda; Kohzy Hiramatsu; Kazushige Takehana, Journal of anatomy, 220, (2) 156 - 163, 2012 02 |
| Efficacy of self-assembled hydrogels composed of positively or negatively charged peptides as scaffolds for cell culture., Nagayasu A; Yokoi H; Minaguchi JA; Hosaka YZ; Ueda H; Takehana K, Journal of biomaterials applications, 26, (6) 651 - 665, 2012 02 |
| Morphological and biochemical analysis of intact and opaque cornea in dogs., Hirooka M; Igarashi O; Nagayasu A; Minaguchi J; Hosaka YZ; Ueda H; Tangkawattana P; Takehana K, Okajimas folia anatomica Japonica, 87, (2) 59 - 64, 2010 08 |
| Comparative study of the properties of tendinocytes derived from three different sites in the equine superficial digital flexor tendon., Hosaka YZ; Uratsuji T; Ueda H; Uehara M; Takehana K, Biomedical research (Tokyo, Japan), 31, (1) 35 - 44, 2010 02 |
| Comparative study of the characteristics and properties of tendinocytes derived from three tendons in the equine forelimb, Yoshinao Z. Hosaka; Hideyuki Takahashi; Takehiro Uratsuji; Prasarn Tangkawattana; Hiromi Ueda; Kazushige Takehana, TISSUE & CELL, CHURCHILL LIVINGSTONE, 42, (1) 9 - 17, 2010 02 |
| A Preliminary Study of the Administrative Effect of Collagen Peptides Derived from Salmon Skin on Dermis Around Bovine Hooves, HOSAKA Yoshinao; GOTOH Chihiro; ABE Kei; NAGAYASU Aya; UEDA Hiromi; TAKEHANA Kazushige, J.Jpn.Vet.Med.Assoc., 日本獸医師会, 63, (1) 38 - 43, 2010 01 20 |
| Site-Dependent Differences in Collagen Lamellae in the Corneal Substantia Propria of Beagle Dogs, Aya Nagayasu; Tomoe Hirayanagi; Yuji Tanaka; Prasarn Tangkawattana; Hiromi Ueda; Kazushige Takehana, JOURNAL OF VETERINARY MEDICAL SCIENCE, JAPAN SOC VET SCI, 71, (9) 1229 - 1231, 2009 09 |
| Morphological changes of the jejunum villi to different diets, HOSHINO Yuji; TAMAKI Kazuyuki; INAYAMA Ichinari; UENO Mitsutoshi; NAGAYASU Aya; HOSAKA Yoshinao; UEDA Hiromi; TAKEHANA Kazushige, Nihon Chikusan Gakkaiho, 公益社団法人 日本畜産学会, 80, (2) 171 - 177, 2009 05 25 |
| Morphological changes of the jejunum villi to di erent diets, 星野 佑治; 玉置 和之; 稲山 一成; 上野 光敏; 永易 彩; 保坂 善真; 植田 弘美; 竹花 一成, 日本畜産学会報 = The Japanese journal of zootechnical science, 日本畜産学会, 80, (2) 171 - 177, 2009 05 |
| Effect of Collagen Oligopeptide Injection on Rabbit Tenositis, Hiromi Ueda; Natsuko Meguri; Jun Minaguchi; Takafumi Watanabe; Aya Nagayasu; Yoshinao Hosaka; Prasarn Tangkawattana; Yasuo Kokai; Kazushige Takehana, JOURNAL OF VETERINARY MEDICAL SCIENCE, JAPAN SOC VET SCI, 70, (12) 1295 - 1300, 2008 12 |
| Morphology of mesenteric arteries in horse having endotoxemia-related colic, HIDAKA Shuhei; UEDA Hiromi; HOSAKA Yoshinao; TAKEHANA Kazushige, Journal of the College of Dairying. Natural science, 酪農学園大学, 33, (1) 99 - 104, 2008 10 |
| Effect of prolyl hydroxyproline on the differentiation of mouse 3T3-L1 preadipocytes in vitro, SAWAYA Satoe; KOYAMA Yoh-ichi; NAGAYASU Aya; HOSAKA Yoshinao; UEDA Hiromi; MAFUNE Naoki; TAKEHANA Kazushige, Journal of the College of Dairying. Natural science, 酪農学園大学, 33, (1) 13 - 18, 2008 10 |
| Morphological analysis of corneal opacity in Shiba dog with GM1 gangliosidosis., Nagayasu A; Nakamura T; Yamato O; Tsuzuki K; Hosaka Y; Ueda H; Tangkawattana P; Takehana K, The Journal of veterinary medical science, 70, (9) 881 - 886, 2008 09 |
| A preliminary study of direct application of atelocollagen into a wound lesion in the dog cornea, Aya Nagayasu; Yoshinao Hosaka; Ayako Yamasaki; Keiko Tsuzuki; Hiromi Ueda; Tomoaki Honda; Kazushige Takehana, CURRENT EYE RESEARCH, INFORMA HEALTHCARE, 33, (9) 727 - 735, 2008 |
| The modulation of collagen fibril assembly and its structure by decorin: an electron microscopic study., Iwasaki S; Hosaka Y; Iwasaki T; Yamamoto K; Nagayasu A; Ueda H; Kokai Y; Takehana K, Arch. Histol. Cytol., 71, (1) 37 - 44, 2008 |
| Graded arrangement of collagen fibrils in the equine superficial digital flexor tendon, Takafumi Watanabe; Yasutada Imamura; Yoshinao Hosaka; Hiromi Ueda; Kazushige Takehana, Connective Tissue Research, 48, (6) 332 - 337, 2007 11 01 |
| Characteristics of collagen fibrils in the entire equine superficial digital flexor tendon, Masaru Sese; Hiromi Ueda; Takafumi Watanabe; Etsuko Yamamoto; Yoshinao Hosaka; Prasarn Tangkawattana; Kazushige Takehana, Okajimas Folia Anatomica Japonica, 84, (3) 111 - 114, 2007 11 |
| Effect of heat on synthesis of gelatinases and pro-inflammatory cytokines in equine tendinocytes., Hosaka Y; Ozoe S; Kirisawa R; Ueda H; Takehana K; Yamaguchi M, Biomedical research (Tokyo, Japan), 27, (5) 233 - 241, 2006 10 |
| Effects of ingestion of collagen peptide on collagen fibrils and Glycosaminoglycans in the dermis, Naoya Matsuda; Yoh-ichi Koyama; Yoshinao Hosaka; Hiromi Ueda; Takafumi Watanabe; Takayuki Araya; Shinkichi Irie; Kazushige Takehana, JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, CENTER ACADEMIC PUBL JAPAN, 52, (3) 211 - 215, 2006 06 |
| Morphological, biochemical and mechanical features of the cranial cruciate and lateral collateral ligaments in dogs, Hiromi Ueda; Takuya Matsukawa; Takafumi Watanabe; Yoshinao Hosaka; Kazushige Takehana, Okajimas Folia Anatomica Japonica, 83, (1) 25 - 31, 2006 |
| Control of the collagen fibril diameter in the equine superficial digital flexor tendon in horses by decorin, T Watanabe; Y Hosaka; E Yamamoto; H Ueda; K Sugawara; H Takahashi; K Takehana, JOURNAL OF VETERINARY MEDICAL SCIENCE, JAPAN SOC VET SCI, 67, (9) 855 - 860, 2005 09 |
| Distribution of TNF receptors and TNF receptor-associated intracellular signaling factors on equine tendinocytes in vitro., Yoshinao Hosaka; Yurie Sakamoto; Rikio Kirisawa; Takafumi Watanabe; Hiromi Ueda; Kazushige Takehana; Mamoru Yamaguchi, The Japanese journal of veterinary research, 52, (3) 135 - 144, 2004 11 |
| Morphological study of the Golgi tendon organ in equine superficial digital flexor tendon., Takafumi Watanabe; Yoshinao Hosaka; Etsuko Yamamoto; Hiromi Ueda; Prasarn Tangkawattana; Kazushige Takehana, Okajimas folia anatomica Japonica, 81, (2-3) 33 - 37, 2004 08 |
| Effect of beta-aminopropionitrile and hyaluronic acid on repair of collagenase-induced injury of the rabbit Achilles tendon, E Yamamoto; D Hata; A Kobayashi; H Ueda; P Tangkawattana; M Oikawa; K Takehana, JOURNAL OF COMPARATIVE PATHOLOGY, ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD, 126, (2-3) 161 - 170, 2002 02 |
| Characteristics of dorsal lingual papillae of the Bactrian camel (Camelus bactrianus), Eerdunchaolu; K Takehana; E Yamamoto; A Kobayashi; G Cao; Baiyin; H Ueda; P Tangkawattana, ANATOMIA HISTOLOGIA EMBRYOLOGIA-JOURNAL OF VETERINARY MEDICINE SERIES C, BLACKWELL WISSENSCHAFTS-VERLAG GMBH, 30, (3) 147 - 151, 2001 06 |
| Immunohistochemical study of the distribution of endocrine cells in the gastrointestinal tract of the camel (Camelus bactrianus), DVM Eerdunchaolu; K Takehana; A Kobayashi; J Yamada; H Ueda; Baiyin; GF Cao; M Abe, EUROPEAN JOURNAL OF MORPHOLOGY, SWETS ZEITLINGER PUBLISHERS, 39, (1) 57 - 63, 2001 02 |
| イヌの角膜成熟に関する形態学的、生化学的解析, 石井陽子; 山本悦子; 植田弘美; 竹花一成, 日本獣医師会雑誌, 54, (11) 843 - 846, 2001 |
| Characteristics of Dorsal Lingual Papillae of the Bactrian Camel (Camelus Bactrianus), EERDUNCHAOLU; K. TAKEHANA; E. YAMAMOTO; A. KOBAYASHI; G. CAO; BAIYIN; H.UEDA; P. TANGKAWATTANA, Anat. Hist. Emb, 30, 1 - 5, 2001 |
| ウマの実験的浅指屈腱炎に対する薬物治療効果の評価, 山本 悦子; 小林 中; 山下 和人; 岩佐 憲二; 吉原 豊彦; 植田 弘美; 竹花 一成, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 128回, 202 - 202, 1999 09 |
Books etc
| 獣医組織学実習マニュアル 第2版, 五味浩司; 吉岡一機; 坂上元栄, 第2章 筋組織, 学窓社, 2022 09 , 9784873627854 |
| 獣医組織学第八版, 日本獣医解剖学会 編, Chapter 8 脈管系；Chapter 9 リンパ器官, 学窓社, 2020 03 |
| 獣医組織学第七版, 植田 弘美, Chapter8 脈管系, 学窓社, 2017 03 |
| 書いて覚える塗って身につく動物解剖学ノート, 植田 弘美, Unit 13 - Unit 20, 講談社, 2016 03 |
Conference Activities & Talks
| ブタ角膜固有質における細胞分布とギャップ結合によるネットワーク構造の形態学的解析, 星野信隆; 細谷実里奈; 植田弘美; 渡邉敬文, 令和4年度 日本顕微鏡学会 北海道支部学術講演会 |
| 北海道産海水魚における脊椎骨の組織学的解析と分類, 祖父江尚哉; 古田智絵; 山田加一朗; 吉川修司; 細谷実里奈; 渡邉敬文; 植田弘美, 令和4年度 日本顕微鏡学会 北海道支部学術講演会 |
| Pathological role of hedgehog signaling in maintenance of the murine oviductal ciliated epithelium, Marina Hosotani; Osamu Ichii; Takashi Namba; Teppei Nakamura; Yasuhiro Hasegawa; Md. Abdul Masum; Takafumi Watanabe; Hiromi Ueda; Yasuhiro Kon, The 8th Congress of Asian Association of Veterinary Anatomists (Asian AVA) |
| 採卵鶏における卵管形態に着目した産卵障害の病態解析, 浜野 爽平; 渡邉 敬文; 岩﨑 智仁; 長谷川 靖洋; 植田 弘美; 細谷 実里奈, 第165回日本獣医学会学術集会 |
| 自己免疫疾患モデルマウスを使用した子宮内膜症の病態解析, 秋田真知子; 渡邉敬文; 植田弘美; 細谷実里奈, 第165回日本獣医学会学術集会 |
| 甘草の過剰摂取に伴うラット腎臓の形態変化, 李垠錫; 細谷実里奈; 渡邉敬文; 植田弘美, 令和3年度日本顕微鏡学会北海道支部学術講演会 |
| ニワトリ速筋線維の各サブタイプを構成するミトコンドリアの三次元 構造解析, 牧田紗智; 岩﨑智仁; 長谷川靖洋; 細谷実里奈; 亀谷清和; 髙橋直紀; 植田弘美; 渡邉 敬文, 令和3年度日本顕微鏡学会北海道支部学術講演会 |
| 甘草過剰摂取により派生する肝線維化および細胆管反応の形態解析, 的場聖礼; 細谷実里奈; 渡邉敬文; 植田弘美, 令和3年度日本顕微鏡学会北海道支部学術講演会 |
| マウス卵管線毛上皮細胞と排卵卵子の相互作用ー加齢と免疫異常は卵子輸送速度を変化させるー, 細谷実里奈; 市居修; 渡邉敬文; 植田弘美; 昆奏寛, 第164回日本獣医学会学術集会 |
| ニワトリ外側腸脛骨筋ミトコンドリアの三次元構造解析, 牧田紗智; 岩崎智仁; 長谷川靖洋; 細谷実里奈; 亀谷清和; 高橋直樹; 植田弘美; 渡邉敬文, 第164回日本獣医学会学術集会 |
| ラットの皮下に存在するFasciaの形態学的観察, 佐野日向子; 岩崎智仁; 長谷川靖洋; 細谷実里奈; 植田弘美; 渡邉敬文, 第164回日本獣医学会学術集会 |
| 北海道産魚類における脊柱の組織学的検討, 山口慧; 古田智絵; 吉川修司; 山田加一朗; 渡邉敬文; 細谷実里奈; 植田弘美, 日本顕微鏡学会第63回シンポジウム 北海道支部学術講演会 |
| マウス卵管の線毛におけるヘッジホッグシグナル伝達経路の発現, 細谷実里奈; 市居修; 中村鉄平; 長谷川靖洋; Md. Abdul Masum; 渡邉敬文; 植田弘美; 昆泰寛, 第163回日本獣医学会学術集会 |
| コットンラットの尾の組織学的特徴および自切機序の解析, 日本獣医学会学術集会講演要旨集, 第163回日本獣医学会学術集会 |
| バーチャル電子顕微鏡データの作製方法と応用について, 渡邉 敬文; 高橋 直紀; 細谷 実里奈; 植田 弘美; 岩崎 智仁; 亀谷 清和, 第163回日本獣医学会学術集会講演要旨集 |
| ドライエイジングによる筋組織の形態変化の解析, 小原千佳、高橋直紀、細谷実里奈、渡邉敬文、植田弘美, 日本顕微鏡学会北海道支部学術講演会 |
| ウシ浅趾屈筋腱分岐部の微細構造の三次元解析, 高橋 直紀; 亀谷 清和; 大多 亮; 植田 弘美; 渡邉 敬文, 日本顕微鏡学会北海道支部学術講演会 |
| マウスの卵巣囊－腹腔連絡路（卵巣囊孔）, 細谷実里奈、市居修、中村鉄平、難波貴志、Ali Elewa Yaser Hosny、渡邉敬文、植田弘美、昆泰寛, 第162回日本獣医学会学術集会 |
| ウシ浅趾屈筋腱分岐部のコラーゲン細線維の走行と超微細構造の３次元解析, 高橋直紀、亀谷清和、大多亮、細谷実里奈、植田弘美、渡邉敬文, 第162回日本獣医学会学術集会 |
| ３次元電子顕微鏡を用いたアキレス腱の腱細胞の細胞質突起の立体構造解析, 羽立洲、高橋直紀、細谷実里奈、植田弘美、渡邉敬文, 第162回日本獣医学会学術集会 |
| 腱分岐部の微細構造の3次元化, 髙橋直紀、亀谷清和、大多亮、植田弘美、渡邉敬文, 日本顕微鏡学会第75回学術講演会 |
| 新型エーラス・ダンロス症候群の皮膚病理解析から得た脆弱な皮膚のcollagen fibrilと糖鎖の構造, 広瀬 拓哉; 古症 知巳; 高橋 有希; 美名口 順; 高橋 直紀; 植田 弘美; 竹花 一成, 日本顕微鏡学会北海道支部学術講演会 |
| 糖尿病の発現に伴う肝臓の組織学的変化, 植田 弘美; 森井 俊貴; 平賀 武夫, 日本顕微鏡学会北海道支部学術講演会 |
| 糖尿病誘発ラットにおける肝線維化の形態学的観察, 植田 弘美; 森井 俊貴; 平賀 武夫, 日本解剖学会第63回東北・北海道連合支部学術集会 |
| 次亜塩素酸ナトリウムを用いた骨格標本作製の試み, 岩崎 聖; 中尾 伊吹; 平賀 武夫; 植田 弘美, 日本解剖学会第62回東北・北海道連合支部学術集会 |
| 糖尿病ラットに発現される肝臓の形態学的観察, 植田 弘美; 平賀 武夫, 日本解剖学会第62回東北・北海道連合支部学術集会 |
Works
| ブタ脾臓の微細血管系の陰性荷電について, 2001 |
| Anionic sites in the blood vessels of the pig spleen, 2001 |
| 十二指腸分泌物の分子生物学的解析, 1999 |
| フタコブラクダ(Camelus Bactrianus)の舌の形態特徴, 1999 |
| 脾臓微細血管系の陰性荷電について, 1999 |
| Molecular biological analysis of secretion in duodenal glands, 1999 |
| Morphological feature in the camel (Camelus Bactrianus) tongue, 1999 |
| Anionic sites in splenic blood vessels, 1999 |
| ラット脾臓の血管内皮細胞および脾索細網細胞の形質膜糖衣における陰性荷電の電顕組織化学的研究, 1998 |
| ラット大動脈のグリコサミノグリカンの電顕組織化学における三重酵素消化法の効果に関する研究, 1998 |
| Electron microscopic histochemical studies of anionic sites on free surface of endothelial cells of blood vessels and reticular cells of splenic cord in rat spleen, 1998 |
| Effects of a triple enzyme digestion method on a diamine reaction for glycosaminoglycans of the rat aorta in electron microscopy, 1998 |
| モルモット脾臓血管内皮における糖質の電顕組織化学的研究, 1993 |
| Electron microscopic histochemical studies on carbohydrates in the endothelium of the guinea pig splenic blood vessels, 1993 |
| 尿素処理超薄切片を使用する包埋後法によるN-硫酸複合糖質の検出法, 1991 |
| A postembedding method using urea-unmasked ultrathin sections fot the electron microscopic detection of N-sulfated glycoconjugates, 1991 |
| ラット脾臓血管系内皮細胞における糖質の電顕細胞化学的研究, 1990 |
| Electron microscopic cytochemistry of carbohydrates in the endothelical cells lining the splenic blood vessels of the rat, 1990 |
Misc
| ラットの皮下に存在するFasciaの形態学的観察, 佐野日向子; 岩崎智仁; 長谷川靖洋; 細谷実里奈; 植田弘美; 渡邉敬文, 日本獣医学会学術集会講演要旨集, 164th (CD-ROM), 2021 |
| ニワトリ外側腸脛骨筋ミトコンドリアの三次元構造解析, 牧田紗智; 岩崎智仁; 長谷川靖洋; 細谷実里奈; 亀谷清和; 高橋直紀; 植田弘美; 渡邉敬文, 日本獣医学会学術集会講演要旨集, 164th (CD-ROM), 2021 |
| ブロイラーのWooden Breast症における線維化メカニズムの研究, 益田侑空; 細谷実里奈; 岩崎智仁; 長谷川靖洋; 川崎武志; 植田弘美; 渡邉敬文, 日本獣医学会学術集会講演要旨集, 164th (CD-ROM), 2021 |
| ニワトリ速筋線維の各サブタイプを構成するミトコンドリアの三次元構造解析, 牧田紗智; 岩崎智仁; 長谷川靖洋; 細谷実里奈; 亀谷清和; 高橋直紀; 植田弘美; 渡邉敬文, 日本顕微鏡学会北海道支部学術講演会講演要旨集, 2021 (CD-ROM), 2021 |
| マウス卵管線毛上皮細胞と排卵卵子の相互作用-加齢と免疫異常は卵子輸送速度を変化させる-, 細谷実里奈; 市居修; 渡邉敬文; 植田弘美; 昆泰寛, 日本獣医学会学術集会講演要旨集, 164th (CD-ROM), 2021 |
| コットンラットの尾の組織学的特徴および自切機序の解析, 中村鉄平; 中村鉄平; 細谷実里奈; 市居修; 入江隆夫; 入江隆夫; 寸田祐嗣; ELEWA Yaser Hosny Ali; 三嶋隆; 渡邉敬文; 植田弘美; 昆泰寛, 日本獣医学会学術集会講演要旨集, 163rd, 2020 |
| マウス卵管の線毛におけるヘッジホッグシグナル伝達経路の発現, 細谷実里奈; 市居修; 中村鉄平; 中村鉄平; 長谷川靖洋; MASUM Md. Abdul; 渡邉敬文; 植田弘美; 昆泰寛, 日本獣医学会学術集会講演要旨集, 163rd, 2020 |
| マウスの卵巣嚢-腹腔連絡路(卵巣嚢孔), 細谷実里奈; 市居修; 中村鉄平; 中村鉄平; 難波貴志; YASER HOSNY Ali Elewa; 渡邉敬文; 植田弘美; 昆泰寛, 日本獣医学会学術集会講演要旨集, 162nd, 2019 |
| 蝸牛の種差に関する鋳型樹脂標本を用いた研究, 城戸 大樹; 髙橋 直紀; 広瀨 拓哉; 福冨 彰; 植田 弘美; 白石 治恵; 竹花 一成, Research of One Health, 2017 06 |
| 豚の肝小葉の経時的な構造変化, 美案口 順; 山本かおる; 植田弘美; 竹花一成, 酪農学園大学紀要, 37, 2013 04 |
| Comparative study of the characteristics and properties of tendinocytes derived from three tendons in the equine forelimb (vol 42, pg 9, 2010), Yoshinao Z. Hosaka; Hideyuki Takahashi; Takehiro Uratsuji; Parasarn Tangkawattana; Hiromi Ueda; Kazushige Takehana, TISSUE & CELL, 42, (4) 273 - 273, 2010 08 , 10.1016/j.tice.2010.08.001 |
| 角膜実質の再生医療 1.ブタ角膜実質細胞の培養法の検討, 永易彩; 角出泰造; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 145th, 2008 03 07 |
| Active Expression of Matrix Metalloproteinase-13 mRNA in the Granulation Tissue of Equine Superficial Digital Flexor Tendinitis, NOMURA Masayuki; HOSAKA Yoshinao; KASASHIMA Yoshinori; UEDA Hiromi; TAKEHANA Kazushige; KUWANO Atsutoshi; ARAI Katsuhiko, Journal of Veterinary Medical Science, 69, (6) 637 - 639, 2007 06 25 , The DNA microarray analysis for matrix metalloproteinase (MMP)-related mRNA expression in equine superficial digital flexor tendinitis indicated that mRNA level of MMP-13 was apparently up-regulated in the tendinitis as compared to normal tendon. <i>In situ</i> hybridization also revealed that fibroblastic cells proliferated in the granulation tissue generated in the tendinitis actively expressed MMP-13 mRNA. On the other hand, in normal tendon, a few fibroblastic cells and vascular components lied in the endotenon barely expressed its mRNA, but other cellular components in the tendon bundle were not positively hybridized. As mentioned above, MMP-13 but not other collagenases or gelatinases, may play an important role in tendon injuries in the racehorses.<br> |
| コラーゲン形態形成におけるデコリンの役割, 岩崎俊輔; 保坂善真; 植田弘美; 小海康夫; 竹花一成, 解剖学雑誌, 82, (2) 76 - 76, 2007 06 01 |
| 3T3‐L1脂肪前駆細胞の分化による脂肪蓄積と形態変化, 沢谷里江; 植田弘美; 保坂善真; 真船直樹; 新谷隆行; 小山洋一; 竹花一成, 解剖学雑誌, 82, (2) 76 - 76, 2007 06 01 |
| ノックダウン細胞を用いた,デコリンとコラーゲン細繊維の相互関連の解析, 保坂善真; 岩崎俊輔; 植田弘美; 小海康夫; 竹花一成, 解剖学雑誌, 82, (2) 76 - 76, 2007 06 01 |
| 筋腱接合部における腱の構造特性について, 渡辺敬文; 今村保忠; 保坂善真; 植田弘美; 竹花一成, 日本顕微鏡学会北海道支部学術講演会プログラム・予稿集, 2006, 2007 02 |
| コラーゲンペプチドの経口摂取がブタ皮膚の細胞外マトリックスに及ぼす効果, 松田直也; 保坂善真; 植田弘美; 竹花一成, 日本顕微鏡学会北海道支部学術講演会講演要旨集, 2007, 2007 |
| イヌの食道における粘膜筋板の形態学的研究, 吉田愛; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 142nd, 2006 08 31 |
| 筋腱接合部のコラーゲン細線維の構造特性について, 渡辺敬文; 今村保忠; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 142nd, 2006 08 31 |
| イヌ顎関節の軟骨組織に含まれる複合糖質の組織化学的研究, 広部真智; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 142nd, 2006 08 31 |
| コラーゲンペプチド投与によるウシの蹄冠および趾間皮膚に対する効果, 阿部敬; 永易彩; 渡辺敬文; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 142nd, 2006 08 31 |
| 腱炎に対するコラーゲンオリゴペプチドの効果, 巡夏子; 美名口順; 渡辺敬文; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 142nd, 2006 08 31 |
| ウマ浅指屈筋腱細胞の性状は存在部位によって異なる, 浦辻雄央; 保坂善真; 桐沢力雄; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 142nd, 2006 08 31 |
| 実験的脂肪肝牛の肝類洞内皮細胞下基底膜の形態的変化, 宮本和吉; 及川伸; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 142nd, 2006 08 31 |
| 趾乳頭腫症Papillomatous Digital Dermatitis(PDD)の皮膚構造と痛覚過敏の原因について, 田中健介; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 142nd, 2006 08 31 |
| 皮膚真皮のコラーゲン線維とグリコサミノグリカンに対するコラーゲンペプチド摂取の効果, 松田 直也; 小山 洋一; 保坂 善真; 植田 弘美; 渡辺 敬文; 新谷 隆行; 入江 伸吉; 竹花 一成, ビタミン, 80, (8) 433 - 433, 2006 08 , https://search-tp.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2006&ichushi_jid=J01262&link_issn=&doc_id=20060831370018&doc_link_id=%2Fcr9vitam%2F2006%2F008008%2F020%2F0433-0433%26dl%3D2&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fcr9vitam%2F2006%2F008008%2F020%2F0433-0433%26dl%3D2&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_5.gif |
| クリプトスポリジウム人工感染子牛に関する治療・予防効果‐小腸粘膜での形態学的評価‐, 竹花一成; 沢谷里江; 保坂善真; 植田弘美; 小岩政照, 北海道獣医師会雑誌, 50, (8) 297 - 41, 2006 08 01 |
| Structure and component alteration of rabbit Achilles tendon in tissue culture, Y Hosaka; H Ueda; T Yamasaki; D Suzuki; N Matsuda; K Takehana, BIOMEDICAL RESEARCH-TOKYO, 26, (6) 279 - 286, 2005 12 , 10.2220/biomedres.26.279, The aim of this study was to investigate alterations of cultured tendon tissues to determine whether tissue culture is a useful method for biological analyses of the tendon. Tendon tissues for tissue culture were isolated from Achilles tendons of rabbits. The tendon segments were placed one segment per well and incubated in growth medium consisting of Dullbecco's modified Eagle's medium supplemented with 5% fetal bovine serum at 37 degrees C in a humidified atmosphere with 5% CO2 for various periods. The alignment of collagen fibrils was preserved for 48 h, but tendon structure has disintegrated at 96 h. Alcian blue staining and gelatine zymography revealed that proteoglycan markedly diminished and that matrix metalloproteinase (MMPs) activity was upregulated sharply at 72 and 96 h. The ratio of collagen fibrils with large diameter had increased and the mean diameter and mass average diameter value had reached maximum at 48 h. The values then decreased and mean diameters at 72 and 96 h were significantly different from that at 48 h. At 96 h, the ratio of collagen fibrils with small diameters had increased and collagen fibrils with large diameters had disappeared. These findings indicate that structural alteration is possible to be induced by disintegration of collagen fibrils and disappearance of glycosaminoglycans from extracellular matrix (ECM), subsequent of upregulation of MMPs activity. Although the study period is limited, the tissue culture method is available for investigating cell-ECM interaction in tendons. |
| 腱の状態によるTNFαとTNF-R1を介する細胞内シグナル伝達因子の分布と発現の差違, 保坂 善真; 桐澤 力雄; 植田 弘美; ほか, The journal of veterinary medical science, 67, (10) 985 - 991, 2005 10 |
| 柴犬のGM1ガングリオシドーシスにおける角膜混濁機序について, 中村泰斗; 大和修; 都築圭子; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 140th, 2005 08 31 |
| ウマの鎖骨画における形態学的研究, 植田弘美; 寺井洋子; 渡辺敬文; 保坂善真; 竹花一成, 日本獣医学会学術集会講演要旨集, 140th, 2005 08 31 |
| コラーゲンペプチドの経口摂取がブタ皮膚の細胞外マトリックスに及ぼす効果, 松田直也; 保坂善真; 植田弘美; 小山洋一; 入江伸吉; 竹花一成, 日本獣医学会学術集会講演要旨集, 140th, 2005 08 31 |
| SHNマウスにおける子宮炎好発とVitamin Aとの関係, 山元明子; 竹花一成; 保坂善真; 植田弘美, 日本獣医学会学術集会講演要旨集, 140th, 2005 08 31 |
| サケ皮膚由来I型コラーゲンゲルを用いたけん細胞培養の展開, 龍尾幸治; 保坂善真; 植田弘美; 真船直樹; 鈴木健; 竹花一成, 日本獣医学会学術集会講演要旨集, 140th, 2005 08 31 |
| けん変性過程での熱,炎症性サイトカインおよびゼラチナーゼの関連, 保坂善真; 尾添紗知子; 桐沢力雄; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 140th, 2005 08 31 |
| ウマ浅指屈筋けんのコラーゲン細線維直径を変化させる分子要因について, 渡辺敬文; 今村保忠; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 140th, 2005 08 31 |
| 若齢期のウマにおける伸筋けんおよび屈筋けんの組織学的変化, 山崎維嗣; 渡辺敬文; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 140th, 2005 08 31 |
| The Molecular Marker NTx Reflecting Changes in Tissue Remodeling Associated with Injury to the Equine Superficial Digital Flexor Tendon, HOSAKA Yoshinao; HIGUCHI Hidetoshi; ONAGA Takenori; NAGATA Hiroshi; KAMIKURA Tomoki; MISUMI Kazuhiro; UEDA Hiromi; Kazushige, Journal of the Japan Veterinary Medical Association, 58, (3) 175 - 179, 2005 03 20 , 骨の代謝マーカーの一つであるI型コラーゲン架橋N-末端テロペプチド濃度（Nx）が馬の腱損傷後の治癒過程をモニタリングするマーカーとして有用であるかを検討した。血清中Nx濃度は腱損傷のない正常群と比較して、腱炎の急性期および亜急性期群で有意に高い値を示した。しかし、慢性期群と正常群間とのNx値には有意差が認められなかった。腱炎による腱のI型コラーゲンの分解は亜急性期までで、慢性期以降では腱組織の再構築が開始されることから、血清中Nx濃度は腱炎によるコラーゲン分解を鋭敏に反映していると考えられた。 |
| RNAi法によるデコリンの遺伝子抑制がコラーゲン細線維のサイズと走行性に与える影響, 保坂善真; 小海康夫; 渡辺敬文; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 139th, 2005 03 01 |
| ウマ浅指屈筋けんのコラーゲン細線維の形態は部位によって変化する, 渡辺敬文; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 139th, 2005 03 01 |
| 膝関節の靭帯の構造特性, 竹花一成; 大友由佳子; 松川拓也; 渡辺敬文; 保坂善真; 植田弘美, 日本獣医学会学術集会講演要旨集, 139th, 2005 03 01 |
| Effects of ingestion of collagen peptide on collagen fibrils and glycosaminoglycans in Achilles tendon, Jun Minaguchi; Yoh-Ichi Koyama; Natsuko Meguri; Yoshinao Hosaka; Hiromi Ueda; Masashi Kusubata; Arisa Hirota; Shinkichi Irie; Naoki Mafune; Kazushige Takehana, Journal of Nutritional Science and Vitaminology, 51, (3) 169 - 174, 2005 , 10.3177/jnsv.51.169, In order to investigate whether the oral ingestion of collagen peptide affects the extracellular matrix of tendon, two doses (0.2 g/kg and 1.0 g/kg body weight) were orally administered daily for 56 d to a rabbit, and both the size of collagen fibrils and the amount of glycosaminoglycans in the Achilles tendon were measured in comparison with those in a rabbit fed with a control protein, lactalbumin, or water alone. Ingestion of collagen peptide or lactalbumin induced a significant increase in collagen fibril diameter and a decrease in fibril density except for a high dose of lactalbumin compared with the water control. A histogram pattern of fibril diameter in a high dose of collagen peptide showed a peak at 160-180 nm, which was not observed in other groups. However the percentage of diameters over 200 nm was the lowest in this group but highest in the low-dose group of collagen peptide. The mean fibril diameter and mass average diameter of a high dose of collagen peptide were significantly smaller than those in a low dose. The amount of dermatan sulphate increased in the high-dose groups, while the amount of hyaluronic acid decreased in rabbits fed with collagen peptide or lactalbumin at either dose. These results suggest that the ingestion of collagen peptide affects the size of collagen fibrils and composition of glycosaminoglycans in the Achilles tendon and thus may improve the mechanical properties of the Achilles tendon. |
| Mechanism of cell death in inflamed superficial digital flexor tendon in the horse, Y Hosaka; H Teraoka; E Yamamoto; H Ueda; K Takehana, JOURNAL OF COMPARATIVE PATHOLOGY, 132, (1) 51 - 58, 2005 01 , 10.1016/j.jcpa.2004.06.006, The aim of the present study was to clarify the presence and determine the role of apoptosis in the degenerative process of the superficial digital flexor tendon (SDFT) in the horse. Samples were obtained from normal and inflamed SDFTs of horses. To detect apoptosis and to identify apoptotic cells, the samples were subjected to immunohistochemical labelling and Western blot analysis. Although a large number of cells in degenerate areas showed positive reactions with caspase-3 and single stranded DNA antibodies, cells in normal tendon samples showed very weak reactions. Excessive apoptosis was confirmed by the results of Western blot analysis, which showed a significant increase in activated caspase-3 protein in the inflamed SDFTs, suggesting that apoptosis occurred in the tendinocytes via a caspase-3-dependent pathways This is the first report of excessive apoptosis in inflamed SDFT of the horse. The results indicate that apoptosis may play an important role in the process of degeneration of the tendon as well as other tissues. (C) 2004 Elsevier Ltd. All rights reserved. |
| RNAi法によるデコリンの産生抑制がコラーゲン細線維のサイズと走行性に与える影響, 保坂善真; 小海康夫; 渡辺敬文; 植田弘美; 竹花一成, 日本結合組織学会学術大会抄録集, 37th, 2005 |
| ウマの浅指屈筋けんにおけるコラーゲン細線維直径の調節, 渡辺敬文; 保坂善真; 山本悦子; 植田弘美; 竹花一成, 日本結合組織学会学術大会抄録集, 37th, 2005 |
| Differences in Tumor Necrosis Factor (TNF)α and TNF Receptor-1-Mediated Intracellular Signaling Factors in Normal, Inflamed and Scar-Formed Horse Tendons, HOSAKA Yoshinao; KIRISAWA Rikio; UEDA Hiromi; YAMAGUCHI Mamoru; TAKEHANA Kazushige, Journal of Veterinary Medical Science, 67, (10) 985 - 991, 2005 , 10.1292/jvms.67.985, Tumor necrosis factor (TNF) receptors (TNF-R)-mediated cell survival or apoptosis has been demonstrated in many cells, but little is known about survival or apoptotic signals via TNF-R1 in tendinocytes. In this study, we focused on four signaling factors, TNFα, TNF-R1, TNFR-associated factor2 (TRAF2) and caspase-3, in order to elucidate the signaling events in tendinocytes. Samples were obtained from normal, inflamed and scar-formed equine superficial digital flexor tendons. To detect these signaling factors, samples were subjected to immunohistochemistry and Western blot analysis, and some samples were also subjected to reverse transcription-polymerase chain reaction (RT-PCR), PCR-Southern blot analysis and in situ hybridization to detect the expression of TNFα mRNA. Distribution of the four factors differed depending on the tendon condition, normal, inflamed or scar-formed. In the normal tendon, large amounts of TRAF2 were found in tendinocytes, but the amounts of TNF-R1 were small. TNFα mRNA was expressed most highly in the inflamed tendon. TNF-R1, which was only faintly detected in the normal tendon, was detected at a high level in the inflamed tendon, and the amounts of TRAF2 and caspase-3 also increased. Activated caspase-3 was only detected in the inflamed tendon. TNFα mRNA was also expressed in the scar-formed tendon, though it showed weak signals, and the expression levels of TNF-R1, TRAF2 and caspase-3 proteins were very low. Two distinct intracellular signaling pathways of TNFα, which lead to cell survival and apoptosis, might be present in tendinocytes mediated through TNF-R1. These results, which reflect the dynamism of TNFα, provide important clues for means to prevent tendinopathy. |
| ウマ浅指屈筋けんにおけるコラーゲン細線維の分岐, 渡辺敬文; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 137th, 2004 03 01 |
| けん細胞へのデコリンアンチセンスオリゴヌクレオチド遺伝子の導入効果, 保坂善真; 桐沢力雄; 真船直樹; 山本悦子; 渡辺敬文; 尾添紗知子; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 137th, 2004 03 01 |
| Production of decorin and TGF-bl in tendinocytes are surpressed by modificaton of decorin gene, Hosaka Y; Kirisawa R; Mafune N; Watanabe T; Ozoe S; Ueda H; Takehana K, Connective tissue, 36, (2) 104 - 104, 2004 |
| Relationship between Serum Vitamin-A Concentration and Hepatic Stellate Cells in Cows with Fatty-liver Disease, 小川美希; 今井克幸; 及川伸; 保坂善真; 植田弘美; 竹花一成, 日本獣医師会雑誌, 57, (10) 639 - 643, 2004 , 10.12935/jvma1951.57.639 |
| シロザケ(Oncorhynchus Keta)の体表粘液は棲息環境に応じて変化するのか, 松本紗生子; 植田弘美; 武田浩郁; 保坂善真; 竹花一成, 日本水産学会北海道支部大会講演要旨集, 2003, 2003 11 28 |
| イヌにおける鎖骨の存在意義, 藤井崇文; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 136th, 2003 09 10 |
| イヌの混濁を呈した角膜の形態学的および生化学的解析, 広岡雅満; 五十嵐治; 山本悦子; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 136th, 2003 09 10 |
| ウマ浅指屈筋けんと総指伸筋けんの形態学的および生化学的性状の比較, 山本悦子; BIRCH H L; GOODSHIP A E; 渡辺敬文; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 136th, 2003 09 10 |
| ウマ浅指屈けん炎におけるマトリックスメタロプロテアーゼ活性, 山本悦子; 渡辺敬文; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 136th, 2003 09 10 |
| けん組織の取込み能について, 保坂善真; 山本悦子; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 136th, 2003 09 10 |
| イヌ膝関節における前十字靭帯と外側側副靭帯の構造的差異, 松川拓也; 山本悦子; 保坂善真; 植田弘美; 中村邦男; 竹花一成, 日本獣医学会学術集会講演要旨集, 136th, 2003 09 10 |
| ウマ浅指屈筋けんの部位差について, 瀬瀬賢; 渡辺敬文; 山本悦子; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 136th, 2003 09 10 |
| 馬浅指屈けん炎におけるマトリックスメタロプロテアーゼ活性, 山本悦子; 渡辺敬文; 保坂善真; 植田弘美; 竹花一成, 北海道獣医師会雑誌, 47, (8) 280 - 280, 2003 08 01 |
| 犬膝関節十字靭帯,側副靭帯の力学的,形態学的および生化学的相異, 松川拓也; 山本悦子; 保坂善真; 植田弘美; 中村邦男; 竹花一成, 北海道獣医師会雑誌, 47, (8) 308 - 308, 2003 08 01 |
| 犬の混濁を呈した角膜の形態学的および生化学的解析, 広岡雅満; 五十嵐治; 山本悦子; 保坂善真; 植田弘美; 竹花一成, 北海道獣医師会雑誌, 47, (8) 292 - 292, 2003 08 01 |
| なぜウマの屈腱炎は骨腱付着部で発症しないのか?, 佐藤 紀子; 保坂 善真; 植田 弘美; 竹花 一成, 解剖学雑誌, 78, (Suppl.) 319 - 319, 2003 04 |
| 腱細胞におけるTNFα細胞内シグナル伝達因子の発現, 保坂 善真; 桐沢 力雄; 岩永 敏彦; 山本 悦子; 植田 弘美; 竹花 一成, 解剖学雑誌, 78, (Suppl.) 319 - 319, 2003 04 |
| 淡水カメへのビタミンD3投与による鰓後体と上皮小体の応答, 荒木 貴裕; 保坂 善真; 植田 弘美; 竹花 一成, 解剖学雑誌, 78, (Suppl.) 320 - 320, 2003 04 |
| 血管壁に発現する陰性荷電について, 植田 弘美; 山本 悦子; 保坂 善真; 竹花 一成, 解剖学雑誌, 78, (Suppl.) 321 - 321, 2003 04 |
| シロザケ(Oncorhynchus keta)卵表面の塩漬に対する形態学的変化, 横澤 香菜; 保坂 善真; 植田 弘美; 武田 浩郁; 竹花 一成, 解剖学雑誌, 78, (Suppl.) 322 - 322, 2003 04 |
| けん炎における腫よう壊死因子(TNF)αの関与, 保坂善真; 桐沢力雄; 岩永敏彦; 山本悦子; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 135th, 2003 03 25 |
| Age-related Changes in the Morphological and Biochemical Characteristics of the Equine Superficial Digital Flexor Tendon, YAMAMOTO Etsuko; HOSAKA Yoshinao; UEDA Hiromi; NAKAMURA Fumio; TAKEHANA Kazushige, Journal of the Japan Veterinary Medical Association, 56, (2) 78 - 82, 2003 , Morphological and biochemical analyses of age-related changes in the superficial digital flexor tendon (SDFT) of thoroughbred horses showed a straightening of collagen-fibril orientation and a decrease in diameter with aging. Type III collagen content increased with aging. In terms of glycosaminoglcan composition, dermatan sulfate decreased and chondroitin sulfate increased. In the SDFT of thoroughbred horses, morphological age-related changes in collagen fibrils are accompanied by changes in biochemical composition. |
| Uptake of erythrocyte iron by tendinocyte in inflamed tendon :, Hosaka Yoshinao; Yamamoto Etsuko; Ueda Hiromi; Takehana Kazushige, Connective tissue, 35, (2) 102 - 102, 2003 |
| Localization of cytokines in tendinocytes of the superficial digital flexor tendon in the horse, Y Hosaka; R Kirisawa; E Yamamoto; H Ueda; H Iwai; K Takehana, JOURNAL OF VETERINARY MEDICAL SCIENCE, 64, (10) 945 - 947, 2002 10 , Although inflammatory activation of cytokines have been analyzed in various tissues, there have only been a few and as-yet-inconclusive studies on cytokines in equine tendons. In this study, the localizations of 4 cytokines (IL-1alpha, IL-1beta, TNFalpha and IFNgamma) in tendinocytes of the equine superficial digital flexor tendon (SDFT) were analyzed by the use of an immunohistochemical method. In inflamed tendons positive staining for all 4 cytokines antibodies were detected in endotedinieum cells and vascular epithelial cells. In contrast, negative or trace immunoreactions were obtained in many tendinocytes in the normal tendon. The variation in cellular immune responses depending on the kind of cytokine may reflect the physiological/pathological condition of the SDFT. |
| 血清中ビタミンA濃度と肝臓星細胞の形態的変化について, 小川美希; 今井克幸; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 134th, 2002 08 20 |
| 新生イヌと成イヌの角膜混濁の相違性, 竹花一成; 山本悦子; 保坂善真; 植田弘美, 日本獣医学会学術集会講演要旨集, 134th, 2002 08 20 |
| ウマ浅指屈けん炎に対するβ‐aminopropionitrile(BAPN)治療効果, 山本悦子; 保坂善真; 山下和人; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 134th, 2002 08 20 |
| ラットのすい臓B細胞分泌に対する酢酸投与の影響, 久松和加; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 134th, 2002 08 20 |
| けんにおける炎症性サイトカインとアポトーシス関連シグナル伝達因子の発現, 保坂善真; 桐沢力雄; 岩永敏彦; 山本悦子; 坂本由梨恵; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 134th, 2002 08 20 |
| ウマの浅指屈けん筋におけるゴルジけん紡錘について, 渡辺敬文; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 134th, 2002 08 20 |
| 肝障害の程度と星細胞(ビタミンA貯蔵細胞)の関係, 竹花一成; 及川伸; 保坂善真; 植田弘美, 北海道獣医師会雑誌, 46, (8) 258 - 258, 2002 08 01 |
| イヌの角膜成熟と細胞外マトリックス成分の相関性, 竹花一成; 山本悦子; 保坂善真; 植田弘美, マトリックス研究会大会, 49th, (1) 75 - 75, 2002 04 , 【目的】新生イヌの角膜は未発達な網膜や輝板を保護するために混濁しており、眼の成熟と共に透明になる.角膜の透明度は角膜固有層のコラーゲン細線維の規則正しい配列とそれらの間の細胞外マトリックスの一種であるグリコサミノグリカン(GAG)の充填が重要であるとされている.しかし,混濁とGAG構成の変化との相関性については明らかにされていない.そこでイヌの角膜の正常変化についてビーグル犬を用い形態学的および生化学的手法により解析した.【方法】形態学的には角膜のコラーゲン細線維の配列状態ならびに直径と単位面積あたりの基質占有割合を算出し,生化学的にはGAGの解析を行った.【結果】新生イヌの角膜は開眼時には混濁しており,その後徐々に混濁は和らぎ,生後3週目前後で完全に透明になった.角膜の主体である角膜固有層のコラーゲン細線維の直径は週齢毎でほぼ均一で0週齢では24.0nm程度,成イヌでは38.0nmであった.コラーゲン細線維の配列は成熟に伴い規則性を有していた.基質占有割合は80%から30%と減少していた.基質のGAG組成は週齢の増加と共にケラタン硫酸(KS)が増加しデルマタン硫酸(DS)が減少していた.【考察】角膜成熟に伴いコラーゲン細線維の太さと規則性の変化が認められ、生化学的には、KSの増加とDSの減少が顕著であった。このことは、コラーゲン細線維に対するKSとDSの作用、すなわちその直径と空間の維持と形成抑制作用に由来するもので、角膜の成熟にはこれらの成分の存在は必要不可欠であることが明らかになった。 |
| けん成熟に対するMMPの作用, 山本悦子; 保坂善真; 植田弘美; 竹花一成, マトリックス研究会大会, 49th, (1) 88 - 88, 2002 04 , 【目的】浅指屈筋腱は腱細胞、コラーゲン細線維と非コラーゲン性マトリックスにより構成されている。ウマの腱における細胞外マトリックスの加齢性変化の研究は、コラーゲンについては多数報告されているが、マトリックス分解系についての報告はほとんどない。そこで、コラーゲンの細胞外分傍系の1つであるマトリツクスメタロブロテアーゼ(MMP)に著目し、腱の加齢性変化とMMPの関与を検索した。【材料および方法】材料は、サラブレッド種(0歳5例、1歳2例、2歳2例、3歳1例、計10例)の浅指屈筋腱中手骨中央部を用いた。形態学的には、電子顕微鏡を用いてコラーゲン細線維の走行と直径の変化を視察した。生化学的には、二次元電気泳動法にてグリコサミノグリカン(GAG)の分離・同定を行った。さらに、Film in situ ZymographyとザイモグラフィーにてMMPの分離・同定を行った。【結果および考察】コラーゲン細線維は0歳では波状に走行しており、3歳では長軸に沿って平行に走行していた。細線維直径は0歳では20nmと100nmが多く、加齢に伴ってそれぞれ60nm、220nmと太くなり、その割合は加齢に伴い細いものが増加した。GAGはヒアルロン酸、コンドロイチン硫酸、デルマタン硫酸で構成されており、加齢に伴いデルマタン硫酸が減少し、コンドロイチン硫酸が増加した。MMPはMMP-2とMMP-9の潜在型と活性型が存在し、加齢に伴いMMP-9が流少し、MMP-2が増加した。以上のことより、腱コラーゲン細線維の加齢に伴う形態変化は、コラーゲン細線維形成調節作用を持つGAGによって起こり、分解系であるMMPも作用していることが示唆された。 |
| ウマ腱細胞におけるアポトーシス発現とサイトカインの関与について, 保坂 善真; 山本 悦子; 植田 弘美; 竹花 一成, 解剖学雑誌, 77, (Suppl.) APPENDIX24 - APPENDIX24, 2002 03 |
| Ultrastructure and function of sertoli cell processes in the Korean native goat, Geon Oh Kwon; Young Seok Park; Seong Ho Lee; Etsuko Yamamoto; Yoshinao Hosaka; Hiromi Ueda; Kazushige Takehana, Okajimas Folia Anatomica Japonica, 79, (4) 121 - 128, 2002 , 10.2535/ofaj.79.121, The infrastructure of Sertoli cell in the testes of Korean native goats was investigated by scanning and transmission electron microscopy to elucidate the relationship between germ cell and Sertoli cell processes in the spermiogenetic cycle. Type-A Sertoli cells at stage V of the spermiogenetic cycle and type-B Sertoli cells at stage Vu of the spermiogenetic cycle were used for analysis. Morphologically, the Sertoli cell processes were classified into sheet-like and slender cord-like processes. The sheet-like process originated solely from the Sertoli cell column while the slender cord-like process projected either from the Sertoli cell column or the sheet-like process. Periodic acid (PA)-thiocarbohydrazide (TCH)-silver protein (SP)-physical development (PD)-positive granules were found diffusively both in the sheet-like and slender cord-like processes near the round spermatid, whereas they had accumulated near the head of the elongated spermatid. The morphological variation and glucoconjugate histochemical reaction of the Sertoli cell processes reflect nourishment, movement and transformation of the spermatogenic cells in accordance with spermiogenesis. |
| RELATION BETWEEN INFLAMMATORY CYTOKINES AND APOTOSIS EXPRESSION IN TENDON : AN ANALYSIS IN THE EQUINE SUPERHCIAL DIGITAL FLEXOR TENDON, Hosaka Yoshinao; Yamamoto Etsuko; Ueda Hiromi; Takehana Kazushige, Connective tissue, 34, (1) 86 - 86, 2002 , 【目的】アポトーシスは多くの組織で発現報告がなされているが、腱組織では明らかにされていない。本研究では腱組織での腱細胞のアポトーシス発現の有無を検索するとともに、正常時および炎症時での発現の差異を明らかにすることを目的とした。加えて、腱組織内での炎症性サイトカイン陽性細胞の分布を検索し、アポトーシス発現との関与を明らかにした。【材料と方法】ウマの浅指屈筋腱中央部を試料として用いた。試料は肉眼的に異常が全く認められなかったものを正常腱とし、出血・腫脹等の炎症像が見られたものを炎症腱とした。アポトーシス発現の検索はTUNEL法と抗Caspase3抗体による免疫組織化学的手法で、サイトカイン陽性細胞の分布は免疫組織化学的手法およびin situ hybridization法で検索した。形態変化については透過型電子顕微鏡で観察した。【結果と考察】正常腱、炎症腱ともにTUNEL陽性細胞およびCaspase3陽性細胞が確認された。正常腱では陽性細胞が散発的に分布していたのに対し、炎症腱では正常腱に比して多数の陽性細胞が分布していた。電顕でも核クロマチンの凝集像を持つ腱細胞が炎症腱で多く認められ、腱組織中でのアポトーシス発現が明らかになった。サイトカイン陽性細胞は正常腱では散発的に分布していたが、炎症腱では組織内に遊走したマクロファージ等の免疫細胞に加え、腱(芽)細胞、腱内膜細胞および血管内皮細胞が張陽性を呈した。腱炎時に腱組織の細胞外マトリックス構成成分が変化することはすでに明らかにされている。炎症による健内の環境変化が腱細胞のサイトカイン産生を促進し、産生されたサイトカインがさらに腱細胞のアポトーシスの誘発に関与していると推察した。 |
| Liver fattening in cattle presenting with ketosis and its correlation with basal membrane inferior to sinusoid endothelial cell., 岸基史; 東伸好; 保坂善真; 植田弘美; 及川伸; 小岩政照; 竹花一成, 家畜診療, 49, (474) 771 - 776, 2002 |
| Micromorphological characteristics of hepatic sinusoidal endothelial cells and their basal laminae in five different animal species, Nobuyo Higashi; Hiromi Ueda; Osamu Yamada; Shin Oikawa; Masateru Koiwa; Prasarn Tangkawattana; Kazushige Takehana, Okajimas Folia Anatomica Japonica, 79, (5) 135 - 142, 2002 , 10.2535/ofaj.79.135, The micromorphological characteristics of hepatic sinusoidal endothelial cells and their basal laminae in five different animal species (cow, sheep, guinea pig, pig, and dog) were studied by electron microscopy. Using transmission electron microscopy, a prominent basal lamina was seen only in cow and sheep. In pig and guinea pig, no prominent basal lamina was evident. Basal lamina-like material was occasionally found in dog. Immunohistochemically, basal lamina was found in cow, sheep, and dog. Using scanning electron microscopy, the size of endothelial fenestrae differed between species. These results may suggest that variation of endothelia and their basal laminae of different species is related to differences in nourishment. |
| ウシ十二指腸における生後の粘膜変化について, 渡辺洋輔; 山本悦子; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 132nd, 2001 09 07 |
| ウマ浅指屈筋けんにおけるアポトーシス発現, 保坂善真; 山本悦子; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 132nd, 2001 09 07 |
| ウマ浅指屈筋けんの加齢性変化 III コラーゲン細線維とGAG,MMPの関連性について, 山本悦子; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 132nd, 2001 09 07 |
| イヌ子宮畜膿症における子宮内膜の形態学的および生化学的性状, 有泉幸雄; 山本悦子; 保坂善真; 植田弘美; 竹花一成, 日本獣医学会学術集会講演要旨集, 132nd, 2001 09 07 |
| 新生イヌと成イヌの角膜混濁の相違性, 竹花一成; 山本悦子; 保坂善真; 植田弘美, 北海道獣医師会雑誌, 45, (8) 287 - 287, 2001 08 01 |
| 犬の経穴の構造について, 大田晃之; 山本悦子; 横沢香菜; 保坂善真; 植田弘美; 竹花一成, 北海道獣医師会雑誌, 45, (8) 285 - 285, 2001 08 01 |
| イヌの鼻涙管の走行について, 源本泰士; 山本悦子; 保坂善真; 植田弘美; 竹花一成, 北海道獣医師会雑誌, 45, (8) 286 - 286, 2001 08 01 |
| Electron microscopic cytochemical studies of anionic sites in the rat spleen, H Ueda; K Takehana; Eerdunchaolu; K Iwasa; O Fujimori; S Shimada, JOURNAL OF VETERINARY MEDICAL SCIENCE, 63, (3) 287 - 291, 2001 03 , 10.1292/jvms.63.287, The distribution patterns of the intensity of negative charge on the free surfaces (glycocalyx of the plasma membrane) of endothelial cells (ECs) in blood vessels and reticular cells (RCs) in the splenic cord of the rat spleen were studied by an electron microscopic cytochemical method using polyethyleneimine (PEI) as a cationic probe. Spleens from adult male rats were perfusion-fixed with 0.5% glutaraldehyde - 4 % paraformaldehyde containing 0.05% cetylpyridinium chloride and then perfused with 0.58 PEI at pH 7.4. On the free surfaces (glycocalyx of the plasma membrane) of the ECs examined, distinct PEI-positive reactions were observed in blood vessels, such as trabecular :arteries, central arteries, arterial capillaries, pulp veins and trabecular veins. These PEI-positive electron-dense substances in the trabecular arteries, central arteries, and trabecular veins took the shape of a band of 170-250 nm in thickness. On the other hand, the corresponding ultrastructure of the ECs lining the splenic sinuses and the RCs in the splenic cord showed exceedingly weak PEI reactions. The PEI-reactive deposits were significantly thinner than those in the above blood vessels. As the thickness of the electron-dense substances can be related to the density of the negative charge, these results suggest that there is a high intensity of negative charge on the free surfaces (glycocalyx of the plasma membrane) of ECs in blood vessels where blood cells and plasma pass into the red pulp ol are discharged from the red pulp. In contrast, the splenic sinuses and RCs, which are the main components of the red pulp, contain weakly negative-charged sites. This may contribute to the microcirculation of the splenic blood vessels and elucidate the possible physiological functions of the spleen, such as blood storage. |
| A histochemical study of the camel (Camelus bactrianus) duodenal glands, K Takehana; Eerdunchaoluo; H Ueda; A Kobayashi; K Iwasa; K Sou, JOURNAL OF VETERINARY MEDICAL SCIENCE, 62, (4) 449 - 452, 2000 04 , 10.1292/jvms.62.449, The complex carbohydrates in the camel duodenal glands were examined histochemically at light and electron microscopic levels. The duodenal glands of the camel were distributed in the submucosa 2 m caudal from the pylorus. These were branched tubuloalveolar glands. The terminal portion of each lobule was formed by only one type of mucous cell. The duodenal gland cells contained acidic and neutral carbohydrates. The mucous cells mainly contained sulfate and carboxyl carbohydrate with sialic acid, and they also contained a few neutral carbohydrates with different saccharide residues such as mannose, glucose, galactose, N-acetyl glucosamine and N-acetyl galactosamine. The results showed that the secretary granules of the duodenal glands in the camel contain mainly acidic carbohydrates. These findings seem to be the morphological characteristics of the duodenal glands in the camel. |
| Secretary Process Examined Morphologically in B-cell Granules of the Rat Pancreas Islet, OKADA Akira; TAKEHANA Kazushige; EERDUNCHAOLU; KOBAYASHI Ataru; UEDA Hiromi; IWASA Kenji, Journal of the College of Dairying. Natural science, 24, (1) 55 - 62, 1999 |
| Secretary process examined morphologically in B-cell granules of the rat pancreas islet., OKADA Akira; TAKEHANA Kazushige; EERDUNCHAOLU; KOBAYASHI Ataru; UEDA Hiromi; IWASA Kenji, Journal of Rakuno Gakuen University, 24, (1) 55 - 62, 1999 |
| Effects of a triple enzyme digestion method on a diamine reaction for glycosaminoglycans of the at aorta in electron microscopy, H Ueda; O Fujimori, JOURNAL OF VETERINARY MEDICAL SCIENCE, 60, (11) 1169 - 1174, 1998 11 , 10.1292/jvms.60.1169, Effects of a triple enzyme digestion method using glycosaminoglycans-degrading enzymes upon a diamine reaction have been tested for electron microscopic histochemical detection of glycosaminoglycans in extracellular matrix of the rat aorta. The triple enzyme digestion method consists of a sequence of chondroitinase B, testicular hyaluronidase and heparitinase. The results obtained by the present experimental and control studies indicated that dermatan sulfates, chondroitin sulfates (A and/or C) or heparan sulfates were apparently observed in various ultrastructural features of aortic extracellular matrix, such as bundle of collagen fibers and soluble matrix of interstitial space. Particularly, we found that both heparan sulfates and chondroitin sulfates (A and/or C) were detected in association with the basal lamina of smooth muscle cells and the external surface of elastic lamina, and in the latter heparan sulfates were frequently recognized as a mass, whereas chondroitin sulfates (A and/or C) were found intermittently along the external surface of elastic lamina. This suggests that the triple enzyme digestion method which combines the glycosaminoglycans-degrading enzymes with the diamine reaction can be postulated to represent efficient and useful technique for precise electron microscopic histochemical detection of the glycosaminoglycans in the extracellular matrix of the rat aorta. |
| Cytochemical studies on carbohydrates in the reticular cells of the rat splenic cord, H Ueda; O Fujimori, ACTA HISTOCHEMICA ET CYTOCHEMICA, 31, (1) 77 - 79, 1998 , 10.1267/ahc.31.77, Carbohydrates in the reticular cells of the rat splenic cord were studied by means of a series of electron microscopic cytochemical methods. The results indicated that the reticular cells contained an appreciable abundance of glycogen particles in the cytoplasm. In the free surface coat of the plasma membrane, the cells included neutral glycoprotein, which were devoid of detectable amounts of acidic glycoconjugates. The possible physiological significance of these carbohydrates is discussed, with special reference to their characteristic cytochemical features examined in the present study. |
| Histochemical analysis of acidic glycoconjugates in the endothelium lining the splenic blood vessels in the rat, H Ueda; O Fujimori; M Abe, ARCHIVES OF HISTOLOGY AND CYTOLOGY, 59, (4) 389 - 397, 1996 10 , 10.1679/aohc.59.389, An electron microscopic analysis with specific histochemical stainings for acidic glycoconjugates was carried out to examine the endothelium lining blood vessels of the rat spleen, Histochemical staining performed was the postembedding high or low iron diamine-thiocarbohydrazide-silver protein-physical development (HID or LID-TCH-SP-PD) method, with or without prior digestion with acidic glycoconjugate-degrading enzymes, such as heparitinase, testicular hyaluronidase, chondroitinase B and neuraminidase. The results indicated that the acidic glycoconjugates in the basal lamina of the endothelial cells lining the four types of blood vessel(central arteries, arterial capillaries, splenic sinuses and pulp veins) were heparan sulfate, chondroitin sulfate A, and/or C, chondroitin sulfate B and sialic acid residues. In the endothelial cells lining the central arteries, arterial capillaries and pulp veins, the surface coat of the luminal plasma membrane included heparan sulfate, chondroitin sulfate A and/or C, chondroitin sulfate B and sialic acid residues, whereas the corresponding ultrastructure of the splenic sinuses was devoid of detectable amounts of acidic glycoconjugates. This suggests that such characteristic histochemical features of the endothelium in the four types of the splenic blood vessel can be related to the possible physiological functions of the spleen. |
| Electron Microscopic Histochemical Studies on Carbohydrates in the Endothelium Lining the Splenic Blood Vessels of the Guinea Pig, HATTORI OSAMU; UEDA HIROMI, The Journal of the Nagoya City University Medical Association, 47, (1) 31 - 54, 1996 02 01 |
| CARBOHYDRATES IN THE EPIDERMAL MUCOUS CELLS OF A FRESH-WATER FISH MASTACEMBELUS-PANCALUS (MASTACEMBELIDAE, PISCES) AS STUDIED BY ELECTRON-MICROSCOPIC CYTOCHEMICAL METHODS, AK MITTAL; O FUJIMORI; H UEDA; K YAMADA, CELL AND TISSUE RESEARCH, 280, (3) 531 - 539, 1995 06 , Carbohydrates in the mucous cells of the epidermis of the fish Mastacembelus pancalus were studied by means of electron-microscopic cytochemical methods using physical development procedures. Three types of mucous cells (types A-C) were differentiated on the basis of the reactivities of the secretory products elaborated by them. The carbohydrate contents of mucous globules predominantly comprised sulfate esters and traces of oxidizable vicinal diols in type-A cells, oxidizable vicinal diols in type-B cells, and moderate amounts of both sulfate esters and oxidizable vicinal diols in type-C cells. Glycogen particles were also found to occur in the cytoplasm of these cells and glycoproteins containing oxidizable vicinal diols were visualized in Golgi cisternae, rough endoplasmic reticulum, nuclear envelopes, and plasma membranes. In the type-A and type-B cells situated in the superficial layers of the epidermis, extensive cisternae of the Golgi apparatus and copious rough endoplasmic reticulum suggested the active syntheses of secretory contents, in contrast to the type-C mucous cells, which displayed poor development of these organelles, in the deeper layers. |
| A POSTEMBEDDING METHOD USING UREA-UNMASKED ULTRATHIN SECTIONS FOR THE ELECTRON-MICROSCOPIC DETECTION OF SULFATED GLYCOCONJUGATES, H UEDA; O FUJIMORI; K YAMADA, ACTA HISTOCHEMICA ET CYTOCHEMICA, 26, (1) 27 - 34, 1993 , 10.1267/ahc.26.27, Using urea-unmasked ultrathin sections, a postembedding staining method in combination with nitrous acid procedure has been established for the efficient visualization of sulfated glycoconjugates in a series of ultrastructures of three salivary glands of the rat. Pieces of the parotid, submandibular and sublingual glands from adult rats were subjected to glutaraldehyde-paraformaldehyde fixation, rinsed, dehydrated and embedded in LR-White resin. Ultrathin sections were prepared, grid-mounted, incubated in urea (6M) aqueous solution, and then stained with an alcian blue (AB) pH 1.0-phosphotungstic acid (PTA) sequence with or without prior treatment with nitrous acid. The results obtained have shown that sulfated glycoconjugates are involved in such ultrastructures as mast cell granules, basal lamina of arteriolar endothelial cells and intercellular matrix of arteriolar smooth muscle cells. |
| CYTOPLASMIC FILAMENTS IN THE ENDOTHELIAL-CELLS OF THE SHEATHED CAPILLARY - AN ULTRASTRUCTURAL AND IMMUNOCYTOCHEMICAL STUDY IN THE PIG SPLEEN, K TAKEHANA; M ABE; M YAMAGUCHI; T UCHIDA; M INAGAKI; K YAMAMOTO; J MASTY; A WINNARD; H UEDA; H MIYATA, ACTA ANATOMICA, 143, (4) 294 - 300, 1992 04 , Cytoplasmic filaments of the endothelial cells of sheathed capillaries in the pig spleen were identified and their ultrastructure was studied. Two types of cytoplasmic filaments were found: intermediate filaments (diameter: 10 nm) which filled most of the interior of the cells, and thin filaments (diameter: 5 nm) which were located just beneath the cell membrane and filled the lateral cytoplasmic processes. In immunocytochemical preparations, the intermediate filaments were positive for vimentin and desmin, and were negative for keratin. Staining of the thin filaments with heavy meromyosin resulted in arrowhead formations. These observations suggest that the intermediate filaments maintain the cytoarchitecture, possibly protecting the cell from structural alterations induced by blood pressure changes. Concurrently, thin filaments may facilitate the passage of red blood cells and blood platelets through the interendothelial fenestrae of the sheathed endothelial cell to the reticular meshwork in the capillary sheath. |
| ULTRASTRUCTURE OF THE RED PULP IN SPLEEN INNERVATION IN HORSE AND PIG, H UEDA; M ABE; K TAKEHANA; K IWASA; T HIRAGA, ACTA ANATOMICA, 141, (2) 151 - 158, 1991 06 , The innervation of the red pulp in the spleen of horse and pig was investigated by electron microscopy. In addition, the neurilemma was studied by immunohistochemistry specific for S-100 protein. In the pig, a large population of smooth-muscle cells extending from the smooth-muscle trabeculae was present in the red pulp. The cytoplasmic processes of reticular cells enwrapped the smooth-muscle cells, and nerve fibres were distributed between the smooth-muscle cells and the reticular cells. The nerve terminals clustered toward the facing of the smooth-muscle cells. Nerve fibres and terminals were not disclosed within the sheathed artery. Immunohistochemically the neurilemma showed a reaction positive for S-100 protein. In the horse, no smooth-muscle cells were noted in the red pulp. The nerve fibres terminated around the cytoplasmic processes of the reticular cells. Nerve fibres and terminals were disclosed within the sheathed artery, and the terminals contained both large and small dense-cored vesicles. Immunohistochemically the neurilemma showed a reaction negative for S-100 protein. These findings support the presence of the axon-bearing reticular cells described earlier in the horse spleen. |
| A HISTOCHEMICAL METHOD FOR THE DEMONSTRATION OF UNSATURATED LIPIDS, O FUJIMORI; H UEDA; K YAMADA, HISTOCHEMICAL JOURNAL, 23, (2) 91 - 99, 1991 02 , A method has been developed for the histochemical demonstration of unsaturated lipids in light microscopy. It is a peracetic acid-thiocarbohydrazide-silver protein sequence followed by a physical development procedure. In the present study on paraffin and cryostat sections of liver, brain and ovary, unsaturated lipids were visualized as distinct reaction products coloured various shades of brown and black. The reaction products are easier to see and the method is more efficient than the peracetic acid-Schiff method. |
| Cytochemical studies on vicinal diol-containing carbohydrates in the endothelium of the rat splenic blood vessels, Ueda, H; Yanase, Y; Fujimoro, O; Yamada, K, Nagoya Medical Journal, 36, (2) 115 - 127, 1991 |
| AN EFFICIENT HISTOCHEMICAL METHOD FOR UNSATURATED LIPIDS USING A SILVER ENHANCEMENT PROCEDURE, O FUJIMORI; H UEDA; K YAMADA, JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 38, (7) 1060 - 1060, 1990 07 |
| ULTRASTRUCTURE OF SPLEEN INNERVATION IN THE HORSE, COW, PIG AND CHICKEN, H UEDA; M ABE; K TAKEHANA; K IWASA; T HIRAGA; N KUDO, JOURNAL OF ELECTRON MICROSCOPY, 39, (2) 129 - 129, 1990 04 |
| Electron microscopic cytochemistry of neutral carbohydrates in the endothelial cells lining the splenic blood vessels of the rat. :, Ueda H.; Yanase Y.; Yamada K., Acta histochemica et cytochemica, 23, (5) 745 - 745, 1990 |
| ELECTRON-MICROSCOPIC STUDIES OF THE INNERVATION AND CELLS ENFOLDING AXONS IN THE HORSE, PIG AND CHICKEN SPLEENS, M ABE; H UEDA; K TAKEHANA; K IWASA; T HIRAGA, JOURNAL OF ELECTRON MICROSCOPY, 38, (4) 277 - 277, 1989 |
| MORPHOLOGY OF THE ENDOTHELIAL-CELL OF THE SHEATHED ARTERY IN THE SPLEEN, H UEDA; M ABE; K TAKEHANA; K IWASA; T HIRAGA, JOURNAL OF ELECTRON MICROSCOPY, 37, (5) 254 - 255, 1988 |
| A COMPARATIVE-STUDY OF THE SHEATHED ARTERY OF HORSE, COW, PIG, DOG, MINK AND CHICKEN SPLEENS, M ABE; H UEDA; K TAKEHANA; K IWASA; T HIRAGA, JOURNAL OF ELECTRON MICROSCOPY, 37, (2) 108 - 108, 1988 |
Awards & Honors
| 2021 09 , 日本獣医解剖学会, 日本獣医解剖学会奨励賞, ニワトリ速筋線維の各サブタイプを構成するミトコンドリアの三次元構造解析 |
| 2019 12 , 公益財団法人日本顕微鏡学会北海道支部, 令和元年日本顕微鏡学会北海道支部学術講演会北海道支部長賞, ドライエイジングによる筋組織の形態変化の解析 |
Research Grants & Projects
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Cloning and functional analysis of taste receptors for sourness and saltiness, 1998, 2000, SHIMADA Shoichi; UEDA Takashi; HIRABAYASHI Yoshifumi; FUJIMORI Osamu; UGAWA Shinya, We have identifieda cDNA encoding a taste receptor for sourness (MDEG1) from a rat circumvallate papilla cDNA library by combined cDNA homology and functional expression approaches. When expressed in Xenopus laevis oocytes, MDEG1 showed characteristics of a proton-gated amiloride-sensitive cation channel. Interestingly, acidic stimulation by acetic acid, the chief ingredient of vinegar, generatedlarger inwardcurrents than that by hydrochloric acidat equal pH.In situ hybridization revealed that the mRNA of MDEG1 was exclusively localized in taste buds. MDEG1 immunoreactivity was found on the cell surface and in the apical portion of taste bud cells, especially in type III cells which are classifiedas gustatory receptor cells. This is the first taste receptor cDNA to be cloned. To isolate another subunit of the sour-taste receptor, we screened a rat circumvallate papilla cDNA library and iden tified MDEG2. Immunohistochemical and electrophysiological studies demonstrated that MDEG2 formed heterooligomeric channels with MDIEG1 at the apical portion of single taste-cells and modified the desensitization of MDEG1 channel. The desensitization rate of the heterooligomer was dramatically altered by a single amino acid substitution of Gly481 in MDEG2 without changing other channel properties. These findings suggest that MDEG2 regulates the sour-taste receptor desensitization contributing to sour-taste adaptation and that Gly481 in MDEG2 is the key amino acidresidue that controls the duration of sourtaste sensation. |
| 脾臓微細血管系の陰性荷電に関する研究 |
| 脾臓微細血管系の複合糖質に関する研究 |
| Study on negative charge in splenic blood vessels |
| Study on carbohydrates in splenic blood vessels |
社会貢献活動情報
Social Contribution
| 2021 04 01 - 2023 03 31 , 公益社団法人日本顕微鏡学会北海道支部支部長# |
| 2019 09 07 - 2019 09 08 , 日本解剖学会第65回東北・北海道連合支部学術集会, 日本解剖学会北海道支部, 日本解剖学会第65回東北・北海道連合支部学術集会を二日間に渡って酪農学園大学の学生ホールにて開催し、私は大会長を務めた。# |
| 2019 08 24 , 市立札幌開成中等教育学校での先端科学特論における講師, 市立札幌開成中等教育学校, 先端科学特論# |
| 2018 10 24 , 動物愛護教室での講師, 北海道宗谷総合振興局長, 稚内市立東小学校での動物愛護教室# |
| 2018 08 21 , 生物教育分科会研修での講師, 広島市食肉衛生検査所, 広島県私立中学・高等学校生物教育研究会# |
| 2018 08 21 , 体験実習での講師, 広島市食肉衛生検査所, 夏休み親子体験教室# |
| 2017 08 26 , 市立札幌開成中等教育学校での先端科学特論における講師, 市立札幌開成中等教育学校, 先端科学特論# |
| 2016 08 27 , 市立札幌開成中等教育学校での先端科学特論における講師, 市立札幌開成中等教育学校, 先端科学特論# |
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