School of Veterinary Medicine, Department of Veterinary Medicine
石﨑隆弘 イシザキ タカヒロ
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Website：Last Update :2025/06/30Researcher Profile and Settings
Name
ISHIZAKI Takahiro
Affiliation (Master)
School of Veterinary Medicine, Department of Veterinary Medicine
Affiliation & Job
College of Veterinary Medicine, Rakuno Gakuen University Unit of Medical Zoology Lecturer
Education
2016 04 - 2020 03 , Nagasaki University, Graduate School of Biomedical Sciences 2010 04 - 2016 03 , Obihiro University of Agriculture and Veterinary Medicine, Faculty of Animal Husbandry
Degree
Ph.D, Nagasaki University BVSc, Obihiro University of Agriculture and Veterinary Medicine
Association Memberships
日本獣医寄生虫学会 日本獣医学会 日本寄生虫学会
Research Activities
Research Areas
Life sciences, Molecular biology, Gene editing Life sciences, Parasitology, Haemoprotozoan
Research Interests
Erythrocyte Invasion Mechanism, Gene Editing, Molecular Parasitology, haemoprotozoan, Babesia, Plasmodium,
Published Papers
A scalable CRISPR-Cas9 gene editing system facilitates CRISPR screens in the malaria parasite Plasmodium berghei, Thorey K Jonsdottir; Martina S Paoletta; Takahiro Ishizaki; Sophia Hernandez; Maria Ivanova; Alicia Herrera Curbelo; Paulina A Saiki; Martin Selinger; Debojyoti Das; Johan Henriksson; Ellen SC Bushell, Nucleic Acids Research, 53, (2) , 2025 01 Erythrocyte membrane protein 3 (EMAP3) is exposed on the surface of the Plasmodium berghei infected red blood cell, Sophia Hernandez; Ravish Rashpa; Thorey K. Jonsdottir; Martina S. Paoletta; Maria Rayón Diaz; Severine Chevalley-Maurel; Takahiro Ishizaki; Chris J. Janse; Blandine Franke-Fayard; Mathieu Broche; Ellen SC Bushell, bioRxiv, 2024 05 CRISPR/Cas9 and genetic screens in malaria parasites: small genomes, big impact., T. Ishizaki; S. Hernandez; M.S. Paoletta; T. Sanderson; E.S.C. Bushell, Biochemical Society Transactions, 2022 05 Isolation of viable Babesia bovis merozoites to study parasite invasion, Hassan Hakimi; Masahito Asada; Takahiro Ishizaki; Shinichiro Kawazu, Scientific Reports, Springer Science and Business Media LLC, 11, (1) 16959 - 16959, 2021 12 Distinct effects on the secretion of MTRAP and AMA1 in Plasmodium yoelii following deletion of acylated pleckstrin homology domain-containing protein, Nattawat Chaiyawong; Takahiro Ishizaki; Hassan Hakimi; Masahito Asada; Kazuhide Yahata; Osamu Kaneko, Parasitology International, Elsevier BV, 102479 - 102479, 2021 10 cAMP-dependent protein kinase regulates secretion of apical membrane antigen 1 (AMA1) in Plasmodium yoelii, Takahiro Ishizaki; Masahito Asada; Hassan Hakimi; Nattawat Chaiyawong; Yuto Kegawa; Kazuhide Yahata; Osamu Kaneko, Parasitology International, Elsevier BV, 85, 102435 - 102435, 2021 08 Development of a stable transgenic Theileria equi parasite expressing an enhanced green fluorescent protein/blasticidin S deaminase, Bumduuren Tuvshintulga; Arifin Budiman Nugraha; Tomoka Mizutani; Mingming Liu; Takahiro Ishizaki; Thillaiampalam Sivakumar; Xuenan Xuan; Naoaki Yokoyama; Ikuo Igarashi, Scientific Reports, Springer Science and Business Media LLC, 11, (1) 9107 - 9107, 2021 04 A novel Plasmodium yoelii pseudokinase, PypPK1, is involved in erythrocyte invasion and exflagellation center formation., Takahiro Ishizaki; Nattawat Chaiyawong; Hassan Hakimi; Masahito Asada; Mayumi Tachibana; Tomoko Ishino; Kazuhide Yahata; Osamu Kaneko, Parasitology international, 76, 102056 - 102056, 2020 06 Plasmodium yoelii Erythrocyte-Binding-like Protein Modulates Host Cell Membrane Structure, Immunity, and Disease Severity., Yu-Chih Peng; Yanwei Qi; Cui Zhang; Xiangyu Yao; Jian Wu; Sittiporn Pattaradilokrat; Lu Xia; Keyla C Tumas; Xiao He; Takahiro Ishizaki; Chen-Feng Qi; Anthony A Holder; Timothy G Myers; Carole A Long; Osamu Kaneko; Jian Li; Xin-Zhuan Su, mBio, 11, (1) , 2020 01 07 Genome Editing of Babesia bovis Using the CRISPR/Cas9 System., Hakimi H; Ishizaki T; Kegawa Y; Kaneko O; Kawazu SI; Asada M, mSphere, 2019 06 Critical role of Erythrocyte Binding-Like protein of the rodent malaria parasite Plasmodium yoelii to establish an irreversible connection with the erythrocyte during invasion., Kegawa Y; Asada M; Ishizaki T; Yahata K; Kaneko O, Parasitology international, 2018 07 Babesia bovis BOV57, a Theileria parva P67 homolog, is an invasion-related, neutralization-sensitive antigen, Takahiro Ishizaki; Thillaiampalam Sivakumar; Kyoko Hayashida; Hitoshi Takemae; Bumduuren Tuvshintulga; Tserendorj Munkhjargal; Azirwan Guswanto; Ikuo Igarashi; Naoaki Yokoyama, INFECTION GENETICS AND EVOLUTION, 54, 138 - 145, 2017 10 RBC invasion and invasion-inhibition assays using free merozoites isolated after cold treatment of Babesia bovis in vitro culture, Takahiro Ishizaki; Thillaiampalam Sivakumar; Kyoko Hayashida; Bumduuren Tuvshintulga; Ikuo Igarashi; Naoaki Yokoyama, EXPERIMENTAL PARASITOLOGY, 166, 10 - 15, 2016 07 Molecular and biochemical characterization of methionine aminopeptidase of Babesia bovis as a potent drug target, Tserendorj Munkhjargal; Takahiro Ishizaki; Azirwan Guswanto; Hitoshi Takemae; Naoaki Yokoyama; Ikuo Igarashi, VETERINARY PARASITOLOGY, 221, 14 - 23, 2016 05 The PCR detection and phylogenetic characterization of Babesia microti in questing ticks in Mongolia, Bumduuren Tuvshintulga; Thillaiampalam Sivakumar; Badgar Battsetseg; Sandag-ochir Narantsatsaral; Batsaikhan Enkhtaivan; Banzragch Battur; Kyoko Hayashida; Kazuhiro Okubo; Takahiro Ishizaki; Noboru Inoue; Ikuo Igarashi; Naoaki Yokoyama, PARASITOLOGY INTERNATIONAL, 64, (6) 527 - 532, 2015 12
Books etc
J-IDEO, 人間だって動物だ！動物から学ぶ人の医療「マラリアの動物実験モデル」(pp.416-419), 中外医学社, 2024 05
Conference Activities & Talks
Prevalence of East Coast Fever (ECF) and efficacy of a traditional medicine in northern Malawi, Gift Kayira; Takahiro Ishizaki; Ryota Matsuyama; Kyoko Hayashida; Haruyuki Hirata; Kohei Makita, 65th Conference of the Japan Society of Veterinary Epidemiology Current Advances in Molecular Parasitology: Towards Understanding How Parasites Maintain Their Lifecycle, Takahiro Ishizaki, 9th One Health Lecture Series 北海道のエゾシカ(Cervus nippon yezoensis)におけるB. divergensの感染状況の把握と進化系統解析, 伊藤雅志; 池田恵子; 石﨑隆弘; 岡本実; 鈴木一由; 平田晴之, 第167回獣医学会学術集会 Involvement of a pseudokinase pPK4 in the exflagellation center formation in Plasmodium yoelii, Edwin Kimeli Too; Nattawa; Chaiyawong; Takahiro Ishizaki,Than; Zin Tun; Minami Baba; Hassan Hakimi; Masahito Asada; Kazuhide Yahata; Osamu Kaneko, 21st International Congress for Tropical Medicine and Malaria Essential role of pleckstrin homology domain-containing protein 1 (PH1) in AMA1 secretion during erythrocyte invasion by Plasmodium yoelii, Thant Zin Tun; Nattawa; Chaiyawong; Takahiro Ishizaki; Masahito Asada; Kazuhide Yahata; Minami Baba; Osamu Kaneko, 21st International Congress for Tropical Medicine and Malaria Phosphatidic acid, converted by Plasmodium yoelii diacylglycerol kinase 1 (PyDGK1), plays a pivotal role in protein secretion and distribution on merozoite surface during erythrocyte invasion, Takahiro Ishizaki; Nattawat Chaiyawong; Thant Zin Tun; Miako Sakaguchi; Masahito Asada; Fuyuki Tokumasu; Kazuhide Yahata; Prakash Srinivasan; Osamu Kaneko, The 22nd Awaji International Forum on Infection and Immunity Plasmodium yoelii cAMP-dependent protein kinase regulates parasite development in the mosquito vector and is essential for infection of the vertebrate host, Nattawat Chaiyawong; Takahiro Ishizaki; Deepti Sarkar; Osamu Kaneko; Prakash Srinivasan, The 22nd Awaji International Forum on Infection and Immunity ホルマリン固定寄生虫標本からのDNA抽出法の確立, 田口 奈緒、浅川 満彦、石﨑 隆弘, 第167回獣医学会学術集会 ホルマリン固定された吸虫標本からのDNA抽出法の確立, 田口奈緒; 浅川満彦; 石﨑隆弘, 第15回蠕虫研究会 最近の酪農学園大学野生動物医学センターWAMCに鑑定依頼されたダニ症例と同運用停止以降の課題, 浅川満彦; 石﨑隆弘, 第31回ダニと疾患のインターフェイスに関するセミナー(SADI) DGKが制御するマラリア原虫生活環, 石﨑隆弘, 第1回Transformative Life Science Meeting Plasmodium yoelii diacylglycerol kinases are required for the merozoite invasion and blood stage development, Takahiro Ishizaki; Nattawat Chaiyawong; Thant Zin Tun; Miako Sakaguchi; Masahito Asada; Fuyuki Tokumasu; Kazuhide Yahata; Osamu Kaneko, 第93回日本寄生虫学会大会 Phenotypic assessment of pseudokinase pPK3 and pPK4 knockout lines in Plasmodium yoelii, Edwin Kimeli Too; Nattawat Chaiyawong; Takahiro Ishizaki; Thant Zin Tun; Minami Baba; Hassan Hakimi; Masahito Asada; Kazuhide Yahata; Osamu Kaneko, The 21st Awaji International Forum on Infection and Immunity The critical role of Pleckstrin Homology domain-containing protein 1 (PH1) during the asexual blood stages of Plasmodium yoelii, Thant Zin Tun; Nattawat Chaiyawong; Takahiro Ishizaki; Masahito Asada; Kazuhide Yahata; Edwin Too; Minami Baba; Osamu Kaneko, The 21st Awaji International Forum on Infection and Immunity High-throughput gene editing system enables CRISPR screens in Plasmodium berghei., T.K. Jonsdottir; M.S. Paoletta; T. Ishizaki; S. Hernandez; M. Ivanova; D. Das; A. Curbelo; P. Saiki; M.R. Diaz; E.S.C. Bushell, XXXIV Molecular Parasitology Meeting Role of cAMP-dependent protein kinase in the mammalian host and mosquito vector, Chaiyawong N; Sarkar D; Jain Y; Ishizaki T; Kaneko O; Srinivasan P, World Malaria Day Symposium 2023 Critical Roles of Pleckstrin-Homology domain-containing protein 1 (PH1) in asexual blood stage of Plasmodium yoelii, T.Z. Tun; N. Cyaiyawong; T. Ishizaki; M. Asada; K. Yahata; M. Baba; O. Kaneko, The 92nd Annual Meeting of the Japanese Society of Parasitology A scalable CRISPR/Cas9 system using short homology repair (HR) template for genetic screens in Plasmodium berghei, T. Ishizaki; E. Bushell, 15th International Congress of Parasitology (ICOPA 2022) Molecular mechanisms of malaria parasite erythrocyte invasion, O. Kaneko; T.Z. Tun; N. Chaiyawong; T. Ishizaki; M. Baba, 28th Molecular parasitology workshop An effective CRISPR/Cas9 system using short length homology repair template for genetic modification of Plasmodium berghei, Takahiro Ishizaki; Ellen Bushell, BioMalPar XVIII cAMP-dependent protein kinase regulates secretion of apical membrane antigen 1 (AMA1) in Plasmodium yoelii, Ishizaki T; Asada M; Hakimi H; Chaiyawong N; Kegawa Y; Yahata K; Kaneko O, The 19th Awaji International Forum on Infection and Immunity Phenotypic characterization of Plasmodium yoelii in which a pseudokinase pPK4 is disrupted, Too EK; Chaiyawong N; Ishizaki T; Hakimi H; Asada M; Yahata K; Kaneko O, The 19th Awaji International Forum on Infection and Immunity Acylated pleckstrin homology domain-containing protein (APH) is required for efficient secretion of microneme proteins during erythrocyte invasion by Plasmodium yoelii, Chaiyawong N; Ishizaki T; Hakimi H; Asada M; Yahata K; Kaneko O, The 19th Awaji International Forum on Infection and Immunity Functional analysis of two diacylglycerol kinases of Plasmodium yoelii, Takahiro Ishizaki; Nattawat Cyaiyawong; Miako Sakaguchi; Fuyuki Tokumasu; Kazuhide Yahata; Osamu Kaneko, The 90th Japan Society of Parasitology and the 32nd Japan Society of Clinical Parasitology Joint Conference A novel Plasmodium yoelii pseudokinase, PypPK1, is involved in erythrocyte invasion and exflagellation center formation, Ishizaki T , Chaiyawong N , Hakimi H , Asada M , Tachibana M , Ishino T , Yahata K , Kaneko O, US-Japan Cooperative Medical Sciences Program: Parasitic Diseases Panel Meeting Characterization of a novel Plasmodium yoelii serine threonine kinase related to exflagellation center formation., Ishizaki T, Chaiyawong N, Tachibana M, Ishino T, Yahata K, Asada M, Kaneko O, The 18th Awaji International Forum on Infection and Immunity A novel Plasmodium yoelii serine threonine kinase related to exflagellation center formation., Ishizaki T, Chaiyawong N, Asada M, Yahata K, Culleton R, Kaneko O, 30th Annual Molecular Parasitology Meeting ネズミマラリア原虫Plasmodium yoeliiのdiacylglycerol kinase(DAGKp)は赤血球侵入関連分子の表面分泌に関わる, 石﨑隆弘 , 麻田正仁 , 徳舛富由樹 , 坂口美亜子 , 矢幡一英 , 金子修, 第27回分子寄生虫学ワークショップ/第17回分子寄生虫・マラリア研究フォーラム合同大会 Uncovering the mechanism of erythrocyte invasion through phenotypic characterization of undefined malaria parasite proteins for their roles in host cell invasion., Suzuki M , Ishizaki T , Asada M , Yahata K , Kaneko O, 第27回分子寄生虫学ワークショップ/第17回分子寄生虫・マラリア研究フォーラム合同大会 Erythrocyte-Binding-Like protein of Plasmodium yoelii is critical for the erythrocyte invasion., Kegawa Y, Asada M, Ishizaki T, Yahata K, Kaneko O, US-Japan Cooperative Medical Sciences Program: Parasitic Disease joint meeting Distinct timing of secretion of the erythrocyte invasion-related molecules from Plasmodium yoelii merozoite., Ishizaki T , Asada M , Yahata K , Kaneko O, The 17th Awaji International Forum on Infection and Immunity. Development of inducible knockout method for Plasmodium yoelii, T. Ishizaki, Wellcome Genome Campus Advanced Courses and Scientific Conferences “Malaria Experimental Genetics” Babesia bovis BOV57, a Theileria parva P67 homolog, is an invasion-related, neutralization-sensitive antigen., Ishizaki T., Sivakumar T., Hayashida K., Igarashi I., Yokoyama N., 28th Molecular Parasitology Meeting EBL secretion dynamics of malaria parasites during erythrocyte invasion., T. Ishizaki, K. Yahata, M. Asada, O. Kaneko, The 13th Nagasaki-Singapore Medical Symposium Functional analysis of Plasmodium yoelii asexual stage kinases., Ishizaki T., Asada M., Kegawa Y., Yahata K., Kaneko O., 24th Molecular parasitology workshop
Misc
Camallanus sp. (Spirudia: Nematoda) obtained from a cichlid tropical fish captive in an aquarium in Sapporo-city, Hokkaido, Japan, Keisuke Okamoto; Takahiro Ishizaki; Mitsuhiko Asakawa, Journal of the Natural History of Aomori, (30) 11 - 12, 2025 03 An overview of research activities of free-ranging wild macaques based on the Wild Animal Medical Center of Rakuno Gakuen University in Japan, Mitsuhiko Asakawa; Takahiro Ishizaki; Munehiro Okamoto; Shin-Ichi Hayama, Journal of the Natural History of Aomori, (30) 5 - 10, 2025 03 An addendum to the overview of research and educational activities based on the papers published from the Wild Animal Medical Center of Rakuno Gakuen University since 2023, Mitsuhiko Asakawa; Takahiro Ishizaki, J. Rakuno Gakuen Univ, 49, (2) 143 - 148, 2025 03 An overview of research activities of captive wild primates based on the Wild Animal Medical Center of Rakuno Gakuen University in Japan, Mitsuhiko Asakawa; Takahiro Ishizaki; Munehiro Okamoto, J. Rakuno Gakuen Univ, 49, (2) 131 - 142, 2025 03 A summary of parasitic insects related cases handled by the Wild Animal Medical Center at Rakuno Gakuen University, Japan, Mitsuhiko Asakawa; Takahiro Ishizaki; Tomoo Yoshino, J. Rakuno Gakuen Univ, 49, (2) 119 - 129, 2025 03 北海道伊達市内で経験された保護猫に認められたツツガムシ刺咬事例について, 田淵祐司; 石﨑隆弘; 浅川満彦, 北海道獣医師会雑誌, 69, (3) 9 - 10, 2025 03 The summary of mite and tick-related cases handled by the Wild Animal Medical Center at Rakuno Gakuen University, Japan, Mitsuhiko Asakawa; Takahiro Ishizaki, J. Rakuno Gakuen Univ, 49, (1) 47 - 58, 2024 10 Annual reports on the educational and outreach activities of the Wild Animal Medical Center at Rakuno Gakuen University for the 2023 and 2024, Mitsuhiko Asakawa; Takahiro Ishizaki; Tomoo Yoshino, J. Rakuno Gakuen Univ, 49, (1) 35 - 45, 2024 10 Cases of parasitic diseases in aquatic animals corresponded by the Wild Animal Medical Center (WAMC) at Rakuno Gakuen University since 2018: Application to core curriculum in education of fish pathology in Japan, Mitsuhiko Asakawa; Takahiro Ishizaki, J. Rakuno Gakuen University, 49, (1) 27 - 33, 2024 10 裂頭条虫類後方ストロビラの形態は教科書的な成熟片節とは異なる, 伊藤萌楓; 加来雅人; 石﨑隆弘; 浅川満彦, 日本獣医師回覧板, (275) 34 - 34, 2024 07 Plasmodium yoeliiのErythrocyte-Binding-Like(EBL)タンパク質は赤血球侵入に必須である, 外川裕人; 外川裕人; 麻田正仁; 麻田正仁; 石崎隆弘; 石崎隆弘; 矢幡一英; 金子修; 金子修, 日本寄生虫学会大会プログラム・抄録集, 87th, 2018 On ice法を用いた赤血球侵入能を有する赤血球外バベシアの精製, 石崎隆弘; SIVAKUMAR Thillaiamapalam; 五十嵐郁男; 横山直明, 日本獣医学会学術集会講演要旨集, 157th, 2014 08 11
Awards & Honors
2023 03 , 日本寄生虫学会, 第32回奨励賞, マラリア原虫の偽キナーゼpPK1に関する研究 2022 03 , The Marine Biological Laboratory, Scholarship award for the program Biology of Parasitism 2021 02 , Alumni Association of Institute of Tropical Medicine, Incentive award, A novel Plasmodium yoelii pseudokinase, PypPK1, is involved in erythrocyte invasion and exflagellation center formation. 2018 04 , Wellcome Genome Campus Advanced Courses and Scientific Conferences, WGC Advanced Courses Travel award
Research Grants & Projects
公益財団法人 秋山記念生命科学振興財団, 研究助成(奨励), 北海道内牧野におけるウシピロプラズマ症の分布とウシ品種間における病態差異の解明, 202506, 202603 酪農学園大学, 学内共同研究, ホルマリン固定吸虫標本からの高純度なDNA抽出法の確立, 202504, 202603 一般財団法人 シオノギ感染症研究振興財団, 次世代育成支援研究助成金, バベシア原虫赤内期必須遺伝子の同定に向けた次世代型CRISPRスクリーニング基盤の構築, 202501, 202512 中辻創智社, 会議開催費助成, 会議開催費助成(第15回蠕虫研究会), 202408 帯広畜産大学原虫病研究センター, 原虫病制圧に向けた国際的共同研究拠点共同研究, ウシバベシア原虫赤内期必須遺伝子の同定に向けた次世代型CRISPRスクリーニング基盤の構築, 202304, 202403 Japan Society for the Promotion of Science, Overseas Research Fellow, Elucidation of the sensing mechanism in malaria parasite that play a role in host environment adaptation, 202105, 202304 Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists, 分裂体期脂質転換酵素による新規『遅延死』モデルの構築, 202104, 202303, 石崎 隆弘 Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity start-up, Elucidation of the DGK regulated erythrocyte invasion mechanism of malaria parasites, 202009, 202203, Takahiro Ishizaki, マラリア原虫は赤血球寄生原虫であり、宿主赤血球へと侵入する際に細胞内シグナル伝達を介して原虫タンパク質の分泌を制御する。ネズミマラリア原虫(Plasmodium yoelii)が保有するジアシルグリセロール(DAG)をホスファチジン酸(PA)へと転換させるジアシルグリセロールキナーゼ(DGK)の1つであるDGK1欠損マラリア原虫は赤血球侵入関連分子の細胞内局在の変化、分子分泌の低下、その結果に伴う原虫のマウスへの感染率と病原性の低下という表現型を示した。ネズミマラリア原虫は2つのDGKを有しており、その2つが相補的な機能を有しているかを検討するためにDGK1/DGK3二重欠損原虫を作出した。DGK1欠損原虫とDGK1/DGK3二重欠損原虫間における赤血球侵入効率には変化がなかったため、DGK1のみがマラリア原虫の赤血球侵入時に必要な酵素であり、赤血球侵入過程において2つのDGK間に相補的作用はないという結論に至った。
またDGK1によって転換されるPAはPHドメインを有するタンパク質と結合することが知られており、原虫が保有するPHドメイン誘導型欠損原虫の作出と解析を並行して実施した。その結果PHドメインを有する分子の1つであるAPHを誘導型欠損した原虫でDGK1欠損原虫同様に赤血球侵入関連分子の表面分泌が阻害される知見を得た。その一方でAPH欠損原虫では赤血球侵入関連分子の細胞内局在の変化は見られなかった。これらの結果からDGK1によって転換されるPAとAPHの相互作用が赤血球侵入関連分子の分泌には必須だが、それら分子の細胞内局在はDGK1由来のPAのみが関与する可能性が示唆された。 Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Signaling mechanism of the apical organelle discharge by malaria parasites during erythrocyte invasion, 201904, 202203, 金子 修; 矢幡 一英; 石崎 隆弘; 麻田 正仁 The Japan Science Society, Sasagawa Scientific Research Grant, マラリア原虫のタンパク質小胞輸送先を決定づける原虫側分子の探索, 202004, 202102 Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows, マラリア原虫の赤血球侵入関連分子分泌調節カスケードの解明, 20170426, 20200331, 石崎 隆弘, マラリア原虫の赤血球侵入は原虫の生存に必須なステップであり、宿主への病原性と密接な繋がりを持つ。赤血球から脱出した原虫はマイクロネームやロプトリーといった細胞小器官から種々の分子を順序立てて分泌しながら赤血球侵入を行う。マイクロネーム分子の分泌には細胞外液組成である低カリウム条件に起因するCa2+シグナルが関与することが明らかとなっているが、分子分泌に至るシグナル経路には不明な点が多い。本研究はネズミマラリア原虫を用いて、後期シゾント期における発現が高いキナーゼ群をDiCre-loxPシステムにより誘導型欠損させた原虫を使って、赤血球侵入に関わる標的キナーゼの同並びに、表現系の得られた分子の下流分子の探索を目的とする。
本年度は、得られたloxP挿入原虫もしくは遺伝子欠損原虫のうち、3つのキナーゼにおいて侵入能力が有意に減少するという結果が得られた。これら得られた3つのキナーゼのうち、2つのキナーゼは侵入能力、マウスへの病原性に対して顕著な減少が見られたことから赤血球侵入関連分子の分泌を免疫蛍光抗体法により評価した。その結果1つのキナーゼでは分子分泌のタイミングが遅れるということ、別のキナーゼでは侵入関連分子群の分泌そのものが低下するということが明らかとなった。これらの結果が引き起こされる分子メカニズムを明らかにするために、次年度はリン酸化プロテオーム解析及び、リン脂質メディエーターの探索を行う予定である。
Educational Activities
Teaching Experience
感染病理学特論(分担), Rakuno Gakuen University 統合獣医学(分担), Rakuno Gakuen University Fish Diseases, Rakuno Gakuen University Practical lecture of infection and pathology, Rakuno Gakuen University Practical training of Veterinary Parasitology, Rakuno Gakuen University Veterinary Parasitology, Rakuno Gakuen University
社会貢献活動情報
Social Contribution
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教員総覧
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Last Update :2025/06/30
Researcher Profile and Settings
Name
| ISHIZAKI Takahiro |
Affiliation (Master)
| School of Veterinary Medicine, Department of Veterinary Medicine |
Affiliation & Job
| College of Veterinary Medicine, Rakuno Gakuen University |
| Unit of Medical Zoology |
| Lecturer |
Education
| 2016 04 - 2020 03 , Nagasaki University, Graduate School of Biomedical Sciences |
| 2010 04 - 2016 03 , Obihiro University of Agriculture and Veterinary Medicine, Faculty of Animal Husbandry |
Degree
| Ph.D, Nagasaki University |
| BVSc, Obihiro University of Agriculture and Veterinary Medicine |
Association Memberships
| 日本獣医寄生虫学会 |
| 日本獣医学会 |
| 日本寄生虫学会 |
Research Activities
Research Areas
| Life sciences, Molecular biology, Gene editing |
| Life sciences, Parasitology, Haemoprotozoan |
Research Interests
| Erythrocyte Invasion Mechanism, |
| Gene Editing, |
| Molecular Parasitology, |
| haemoprotozoan, |
| Babesia, |
| Plasmodium, |
Published Papers
| A scalable CRISPR-Cas9 gene editing system facilitates CRISPR screens in the malaria parasite Plasmodium berghei, Thorey K Jonsdottir; Martina S Paoletta; Takahiro Ishizaki; Sophia Hernandez; Maria Ivanova; Alicia Herrera Curbelo; Paulina A Saiki; Martin Selinger; Debojyoti Das; Johan Henriksson; Ellen SC Bushell, Nucleic Acids Research, 53, (2) , 2025 01 |
| Erythrocyte membrane protein 3 (EMAP3) is exposed on the surface of the Plasmodium berghei infected red blood cell, Sophia Hernandez; Ravish Rashpa; Thorey K. Jonsdottir; Martina S. Paoletta; Maria Rayón Diaz; Severine Chevalley-Maurel; Takahiro Ishizaki; Chris J. Janse; Blandine Franke-Fayard; Mathieu Broche; Ellen SC Bushell, bioRxiv, 2024 05 |
| CRISPR/Cas9 and genetic screens in malaria parasites: small genomes, big impact., T. Ishizaki; S. Hernandez; M.S. Paoletta; T. Sanderson; E.S.C. Bushell, Biochemical Society Transactions, 2022 05 |
| Isolation of viable Babesia bovis merozoites to study parasite invasion, Hassan Hakimi; Masahito Asada; Takahiro Ishizaki; Shinichiro Kawazu, Scientific Reports, Springer Science and Business Media LLC, 11, (1) 16959 - 16959, 2021 12 |
| Distinct effects on the secretion of MTRAP and AMA1 in Plasmodium yoelii following deletion of acylated pleckstrin homology domain-containing protein, Nattawat Chaiyawong; Takahiro Ishizaki; Hassan Hakimi; Masahito Asada; Kazuhide Yahata; Osamu Kaneko, Parasitology International, Elsevier BV, 102479 - 102479, 2021 10 |
| cAMP-dependent protein kinase regulates secretion of apical membrane antigen 1 (AMA1) in Plasmodium yoelii, Takahiro Ishizaki; Masahito Asada; Hassan Hakimi; Nattawat Chaiyawong; Yuto Kegawa; Kazuhide Yahata; Osamu Kaneko, Parasitology International, Elsevier BV, 85, 102435 - 102435, 2021 08 |
| Development of a stable transgenic Theileria equi parasite expressing an enhanced green fluorescent protein/blasticidin S deaminase, Bumduuren Tuvshintulga; Arifin Budiman Nugraha; Tomoka Mizutani; Mingming Liu; Takahiro Ishizaki; Thillaiampalam Sivakumar; Xuenan Xuan; Naoaki Yokoyama; Ikuo Igarashi, Scientific Reports, Springer Science and Business Media LLC, 11, (1) 9107 - 9107, 2021 04 |
| A novel Plasmodium yoelii pseudokinase, PypPK1, is involved in erythrocyte invasion and exflagellation center formation., Takahiro Ishizaki; Nattawat Chaiyawong; Hassan Hakimi; Masahito Asada; Mayumi Tachibana; Tomoko Ishino; Kazuhide Yahata; Osamu Kaneko, Parasitology international, 76, 102056 - 102056, 2020 06 |
| Plasmodium yoelii Erythrocyte-Binding-like Protein Modulates Host Cell Membrane Structure, Immunity, and Disease Severity., Yu-Chih Peng; Yanwei Qi; Cui Zhang; Xiangyu Yao; Jian Wu; Sittiporn Pattaradilokrat; Lu Xia; Keyla C Tumas; Xiao He; Takahiro Ishizaki; Chen-Feng Qi; Anthony A Holder; Timothy G Myers; Carole A Long; Osamu Kaneko; Jian Li; Xin-Zhuan Su, mBio, 11, (1) , 2020 01 07 |
| Genome Editing of Babesia bovis Using the CRISPR/Cas9 System., Hakimi H; Ishizaki T; Kegawa Y; Kaneko O; Kawazu SI; Asada M, mSphere, 2019 06 |
| Critical role of Erythrocyte Binding-Like protein of the rodent malaria parasite Plasmodium yoelii to establish an irreversible connection with the erythrocyte during invasion., Kegawa Y; Asada M; Ishizaki T; Yahata K; Kaneko O, Parasitology international, 2018 07 |
| Babesia bovis BOV57, a Theileria parva P67 homolog, is an invasion-related, neutralization-sensitive antigen, Takahiro Ishizaki; Thillaiampalam Sivakumar; Kyoko Hayashida; Hitoshi Takemae; Bumduuren Tuvshintulga; Tserendorj Munkhjargal; Azirwan Guswanto; Ikuo Igarashi; Naoaki Yokoyama, INFECTION GENETICS AND EVOLUTION, 54, 138 - 145, 2017 10 |
| RBC invasion and invasion-inhibition assays using free merozoites isolated after cold treatment of Babesia bovis in vitro culture, Takahiro Ishizaki; Thillaiampalam Sivakumar; Kyoko Hayashida; Bumduuren Tuvshintulga; Ikuo Igarashi; Naoaki Yokoyama, EXPERIMENTAL PARASITOLOGY, 166, 10 - 15, 2016 07 |
| Molecular and biochemical characterization of methionine aminopeptidase of Babesia bovis as a potent drug target, Tserendorj Munkhjargal; Takahiro Ishizaki; Azirwan Guswanto; Hitoshi Takemae; Naoaki Yokoyama; Ikuo Igarashi, VETERINARY PARASITOLOGY, 221, 14 - 23, 2016 05 |
| The PCR detection and phylogenetic characterization of Babesia microti in questing ticks in Mongolia, Bumduuren Tuvshintulga; Thillaiampalam Sivakumar; Badgar Battsetseg; Sandag-ochir Narantsatsaral; Batsaikhan Enkhtaivan; Banzragch Battur; Kyoko Hayashida; Kazuhiro Okubo; Takahiro Ishizaki; Noboru Inoue; Ikuo Igarashi; Naoaki Yokoyama, PARASITOLOGY INTERNATIONAL, 64, (6) 527 - 532, 2015 12 |
Books etc
| J-IDEO, 人間だって動物だ！動物から学ぶ人の医療「マラリアの動物実験モデル」(pp.416-419), 中外医学社, 2024 05 |
Conference Activities & Talks
| Prevalence of East Coast Fever (ECF) and efficacy of a traditional medicine in northern Malawi, Gift Kayira; Takahiro Ishizaki; Ryota Matsuyama; Kyoko Hayashida; Haruyuki Hirata; Kohei Makita, 65th Conference of the Japan Society of Veterinary Epidemiology |
| Current Advances in Molecular Parasitology: Towards Understanding How Parasites Maintain Their Lifecycle, Takahiro Ishizaki, 9th One Health Lecture Series |
| 北海道のエゾシカ(Cervus nippon yezoensis)におけるB. divergensの感染状況の把握と進化系統解析, 伊藤雅志; 池田恵子; 石﨑隆弘; 岡本実; 鈴木一由; 平田晴之, 第167回獣医学会学術集会 |
| Involvement of a pseudokinase pPK4 in the exflagellation center formation in Plasmodium yoelii, Edwin Kimeli Too; Nattawa; Chaiyawong; Takahiro Ishizaki,Than; Zin Tun; Minami Baba; Hassan Hakimi; Masahito Asada; Kazuhide Yahata; Osamu Kaneko, 21st International Congress for Tropical Medicine and Malaria |
| Essential role of pleckstrin homology domain-containing protein 1 (PH1) in AMA1 secretion during erythrocyte invasion by Plasmodium yoelii, Thant Zin Tun; Nattawa; Chaiyawong; Takahiro Ishizaki; Masahito Asada; Kazuhide Yahata; Minami Baba; Osamu Kaneko, 21st International Congress for Tropical Medicine and Malaria |
| Phosphatidic acid, converted by Plasmodium yoelii diacylglycerol kinase 1 (PyDGK1), plays a pivotal role in protein secretion and distribution on merozoite surface during erythrocyte invasion, Takahiro Ishizaki; Nattawat Chaiyawong; Thant Zin Tun; Miako Sakaguchi; Masahito Asada; Fuyuki Tokumasu; Kazuhide Yahata; Prakash Srinivasan; Osamu Kaneko, The 22nd Awaji International Forum on Infection and Immunity |
| Plasmodium yoelii cAMP-dependent protein kinase regulates parasite development in the mosquito vector and is essential for infection of the vertebrate host, Nattawat Chaiyawong; Takahiro Ishizaki; Deepti Sarkar; Osamu Kaneko; Prakash Srinivasan, The 22nd Awaji International Forum on Infection and Immunity |
| ホルマリン固定寄生虫標本からのDNA抽出法の確立, 田口 奈緒、浅川 満彦、石﨑 隆弘, 第167回獣医学会学術集会 |
| ホルマリン固定された吸虫標本からのDNA抽出法の確立, 田口奈緒; 浅川満彦; 石﨑隆弘, 第15回蠕虫研究会 |
| 最近の酪農学園大学野生動物医学センターWAMCに鑑定依頼されたダニ症例と同運用停止以降の課題, 浅川満彦; 石﨑隆弘, 第31回ダニと疾患のインターフェイスに関するセミナー(SADI) |
| DGKが制御するマラリア原虫生活環, 石﨑隆弘, 第1回Transformative Life Science Meeting |
| Plasmodium yoelii diacylglycerol kinases are required for the merozoite invasion and blood stage development, Takahiro Ishizaki; Nattawat Chaiyawong; Thant Zin Tun; Miako Sakaguchi; Masahito Asada; Fuyuki Tokumasu; Kazuhide Yahata; Osamu Kaneko, 第93回日本寄生虫学会大会 |
| Phenotypic assessment of pseudokinase pPK3 and pPK4 knockout lines in Plasmodium yoelii, Edwin Kimeli Too; Nattawat Chaiyawong; Takahiro Ishizaki; Thant Zin Tun; Minami Baba; Hassan Hakimi; Masahito Asada; Kazuhide Yahata; Osamu Kaneko, The 21st Awaji International Forum on Infection and Immunity |
| The critical role of Pleckstrin Homology domain-containing protein 1 (PH1) during the asexual blood stages of Plasmodium yoelii, Thant Zin Tun; Nattawat Chaiyawong; Takahiro Ishizaki; Masahito Asada; Kazuhide Yahata; Edwin Too; Minami Baba; Osamu Kaneko, The 21st Awaji International Forum on Infection and Immunity |
| High-throughput gene editing system enables CRISPR screens in Plasmodium berghei., T.K. Jonsdottir; M.S. Paoletta; T. Ishizaki; S. Hernandez; M. Ivanova; D. Das; A. Curbelo; P. Saiki; M.R. Diaz; E.S.C. Bushell, XXXIV Molecular Parasitology Meeting |
| Role of cAMP-dependent protein kinase in the mammalian host and mosquito vector, Chaiyawong N; Sarkar D; Jain Y; Ishizaki T; Kaneko O; Srinivasan P, World Malaria Day Symposium 2023 |
| Critical Roles of Pleckstrin-Homology domain-containing protein 1 (PH1) in asexual blood stage of Plasmodium yoelii, T.Z. Tun; N. Cyaiyawong; T. Ishizaki; M. Asada; K. Yahata; M. Baba; O. Kaneko, The 92nd Annual Meeting of the Japanese Society of Parasitology |
| A scalable CRISPR/Cas9 system using short homology repair (HR) template for genetic screens in Plasmodium berghei, T. Ishizaki; E. Bushell, 15th International Congress of Parasitology (ICOPA 2022) |
| Molecular mechanisms of malaria parasite erythrocyte invasion, O. Kaneko; T.Z. Tun; N. Chaiyawong; T. Ishizaki; M. Baba, 28th Molecular parasitology workshop |
| An effective CRISPR/Cas9 system using short length homology repair template for genetic modification of Plasmodium berghei, Takahiro Ishizaki; Ellen Bushell, BioMalPar XVIII |
| cAMP-dependent protein kinase regulates secretion of apical membrane antigen 1 (AMA1) in Plasmodium yoelii, Ishizaki T; Asada M; Hakimi H; Chaiyawong N; Kegawa Y; Yahata K; Kaneko O, The 19th Awaji International Forum on Infection and Immunity |
| Phenotypic characterization of Plasmodium yoelii in which a pseudokinase pPK4 is disrupted, Too EK; Chaiyawong N; Ishizaki T; Hakimi H; Asada M; Yahata K; Kaneko O, The 19th Awaji International Forum on Infection and Immunity |
| Acylated pleckstrin homology domain-containing protein (APH) is required for efficient secretion of microneme proteins during erythrocyte invasion by Plasmodium yoelii, Chaiyawong N; Ishizaki T; Hakimi H; Asada M; Yahata K; Kaneko O, The 19th Awaji International Forum on Infection and Immunity |
| Functional analysis of two diacylglycerol kinases of Plasmodium yoelii, Takahiro Ishizaki; Nattawat Cyaiyawong; Miako Sakaguchi; Fuyuki Tokumasu; Kazuhide Yahata; Osamu Kaneko, The 90th Japan Society of Parasitology and the 32nd Japan Society of Clinical Parasitology Joint Conference |
| A novel Plasmodium yoelii pseudokinase, PypPK1, is involved in erythrocyte invasion and exflagellation center formation, Ishizaki T , Chaiyawong N , Hakimi H , Asada M , Tachibana M , Ishino T , Yahata K , Kaneko O, US-Japan Cooperative Medical Sciences Program: Parasitic Diseases Panel Meeting |
| Characterization of a novel Plasmodium yoelii serine threonine kinase related to exflagellation center formation., Ishizaki T, Chaiyawong N, Tachibana M, Ishino T, Yahata K, Asada M, Kaneko O, The 18th Awaji International Forum on Infection and Immunity |
| A novel Plasmodium yoelii serine threonine kinase related to exflagellation center formation., Ishizaki T, Chaiyawong N, Asada M, Yahata K, Culleton R, Kaneko O, 30th Annual Molecular Parasitology Meeting |
| ネズミマラリア原虫Plasmodium yoeliiのdiacylglycerol kinase(DAGKp)は赤血球侵入関連分子の表面分泌に関わる, 石﨑隆弘 , 麻田正仁 , 徳舛富由樹 , 坂口美亜子 , 矢幡一英 , 金子修, 第27回分子寄生虫学ワークショップ/第17回分子寄生虫・マラリア研究フォーラム合同大会 |
| Uncovering the mechanism of erythrocyte invasion through phenotypic characterization of undefined malaria parasite proteins for their roles in host cell invasion., Suzuki M , Ishizaki T , Asada M , Yahata K , Kaneko O, 第27回分子寄生虫学ワークショップ/第17回分子寄生虫・マラリア研究フォーラム合同大会 |
| Erythrocyte-Binding-Like protein of Plasmodium yoelii is critical for the erythrocyte invasion., Kegawa Y, Asada M, Ishizaki T, Yahata K, Kaneko O, US-Japan Cooperative Medical Sciences Program: Parasitic Disease joint meeting |
| Distinct timing of secretion of the erythrocyte invasion-related molecules from Plasmodium yoelii merozoite., Ishizaki T , Asada M , Yahata K , Kaneko O, The 17th Awaji International Forum on Infection and Immunity. |
| Development of inducible knockout method for Plasmodium yoelii, T. Ishizaki, Wellcome Genome Campus Advanced Courses and Scientific Conferences “Malaria Experimental Genetics” |
| Babesia bovis BOV57, a Theileria parva P67 homolog, is an invasion-related, neutralization-sensitive antigen., Ishizaki T., Sivakumar T., Hayashida K., Igarashi I., Yokoyama N., 28th Molecular Parasitology Meeting |
| EBL secretion dynamics of malaria parasites during erythrocyte invasion., T. Ishizaki, K. Yahata, M. Asada, O. Kaneko, The 13th Nagasaki-Singapore Medical Symposium |
| Functional analysis of Plasmodium yoelii asexual stage kinases., Ishizaki T., Asada M., Kegawa Y., Yahata K., Kaneko O., 24th Molecular parasitology workshop |
Misc
| Camallanus sp. (Spirudia: Nematoda) obtained from a cichlid tropical fish captive in an aquarium in Sapporo-city, Hokkaido, Japan, Keisuke Okamoto; Takahiro Ishizaki; Mitsuhiko Asakawa, Journal of the Natural History of Aomori, (30) 11 - 12, 2025 03 |
| An overview of research activities of free-ranging wild macaques based on the Wild Animal Medical Center of Rakuno Gakuen University in Japan, Mitsuhiko Asakawa; Takahiro Ishizaki; Munehiro Okamoto; Shin-Ichi Hayama, Journal of the Natural History of Aomori, (30) 5 - 10, 2025 03 |
| An addendum to the overview of research and educational activities based on the papers published from the Wild Animal Medical Center of Rakuno Gakuen University since 2023, Mitsuhiko Asakawa; Takahiro Ishizaki, J. Rakuno Gakuen Univ, 49, (2) 143 - 148, 2025 03 |
| An overview of research activities of captive wild primates based on the Wild Animal Medical Center of Rakuno Gakuen University in Japan, Mitsuhiko Asakawa; Takahiro Ishizaki; Munehiro Okamoto, J. Rakuno Gakuen Univ, 49, (2) 131 - 142, 2025 03 |
| A summary of parasitic insects related cases handled by the Wild Animal Medical Center at Rakuno Gakuen University, Japan, Mitsuhiko Asakawa; Takahiro Ishizaki; Tomoo Yoshino, J. Rakuno Gakuen Univ, 49, (2) 119 - 129, 2025 03 |
| 北海道伊達市内で経験された保護猫に認められたツツガムシ刺咬事例について, 田淵祐司; 石﨑隆弘; 浅川満彦, 北海道獣医師会雑誌, 69, (3) 9 - 10, 2025 03 |
| The summary of mite and tick-related cases handled by the Wild Animal Medical Center at Rakuno Gakuen University, Japan, Mitsuhiko Asakawa; Takahiro Ishizaki, J. Rakuno Gakuen Univ, 49, (1) 47 - 58, 2024 10 |
| Annual reports on the educational and outreach activities of the Wild Animal Medical Center at Rakuno Gakuen University for the 2023 and 2024, Mitsuhiko Asakawa; Takahiro Ishizaki; Tomoo Yoshino, J. Rakuno Gakuen Univ, 49, (1) 35 - 45, 2024 10 |
| Cases of parasitic diseases in aquatic animals corresponded by the Wild Animal Medical Center (WAMC) at Rakuno Gakuen University since 2018: Application to core curriculum in education of fish pathology in Japan, Mitsuhiko Asakawa; Takahiro Ishizaki, J. Rakuno Gakuen University, 49, (1) 27 - 33, 2024 10 |
| 裂頭条虫類後方ストロビラの形態は教科書的な成熟片節とは異なる, 伊藤萌楓; 加来雅人; 石﨑隆弘; 浅川満彦, 日本獣医師回覧板, (275) 34 - 34, 2024 07 |
| Plasmodium yoeliiのErythrocyte-Binding-Like(EBL)タンパク質は赤血球侵入に必須である, 外川裕人; 外川裕人; 麻田正仁; 麻田正仁; 石崎隆弘; 石崎隆弘; 矢幡一英; 金子修; 金子修, 日本寄生虫学会大会プログラム・抄録集, 87th, 2018 |
| On ice法を用いた赤血球侵入能を有する赤血球外バベシアの精製, 石崎隆弘; SIVAKUMAR Thillaiamapalam; 五十嵐郁男; 横山直明, 日本獣医学会学術集会講演要旨集, 157th, 2014 08 11 |
Awards & Honors
| 2023 03 , 日本寄生虫学会, 第32回奨励賞, マラリア原虫の偽キナーゼpPK1に関する研究 |
| 2022 03 , The Marine Biological Laboratory, Scholarship award for the program Biology of Parasitism |
| 2021 02 , Alumni Association of Institute of Tropical Medicine, Incentive award, A novel Plasmodium yoelii pseudokinase, PypPK1, is involved in erythrocyte invasion and exflagellation center formation. |
| 2018 04 , Wellcome Genome Campus Advanced Courses and Scientific Conferences, WGC Advanced Courses Travel award |
Research Grants & Projects
| 公益財団法人 秋山記念生命科学振興財団, 研究助成(奨励), 北海道内牧野におけるウシピロプラズマ症の分布とウシ品種間における病態差異の解明, 202506, 202603 |
| 酪農学園大学, 学内共同研究, ホルマリン固定吸虫標本からの高純度なDNA抽出法の確立, 202504, 202603 |
| 一般財団法人 シオノギ感染症研究振興財団, 次世代育成支援研究助成金, バベシア原虫赤内期必須遺伝子の同定に向けた次世代型CRISPRスクリーニング基盤の構築, 202501, 202512 |
| 中辻創智社, 会議開催費助成, 会議開催費助成(第15回蠕虫研究会), 202408 |
| 帯広畜産大学原虫病研究センター, 原虫病制圧に向けた国際的共同研究拠点共同研究, ウシバベシア原虫赤内期必須遺伝子の同定に向けた次世代型CRISPRスクリーニング基盤の構築, 202304, 202403 |
| Japan Society for the Promotion of Science, Overseas Research Fellow, Elucidation of the sensing mechanism in malaria parasite that play a role in host environment adaptation, 202105, 202304 |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists, 分裂体期脂質転換酵素による新規『遅延死』モデルの構築, 202104, 202303, 石崎 隆弘 |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity start-up, Elucidation of the DGK regulated erythrocyte invasion mechanism of malaria parasites, 202009, 202203, Takahiro Ishizaki, マラリア原虫は赤血球寄生原虫であり、宿主赤血球へと侵入する際に細胞内シグナル伝達を介して原虫タンパク質の分泌を制御する。ネズミマラリア原虫(Plasmodium yoelii)が保有するジアシルグリセロール(DAG)をホスファチジン酸(PA)へと転換させるジアシルグリセロールキナーゼ(DGK)の1つであるDGK1欠損マラリア原虫は赤血球侵入関連分子の細胞内局在の変化、分子分泌の低下、その結果に伴う原虫のマウスへの感染率と病原性の低下という表現型を示した。ネズミマラリア原虫は2つのDGKを有しており、その2つが相補的な機能を有しているかを検討するためにDGK1/DGK3二重欠損原虫を作出した。DGK1欠損原虫とDGK1/DGK3二重欠損原虫間における赤血球侵入効率には変化がなかったため、DGK1のみがマラリア原虫の赤血球侵入時に必要な酵素であり、赤血球侵入過程において2つのDGK間に相補的作用はないという結論に至った。 またDGK1によって転換されるPAはPHドメインを有するタンパク質と結合することが知られており、原虫が保有するPHドメイン誘導型欠損原虫の作出と解析を並行して実施した。その結果PHドメインを有する分子の1つであるAPHを誘導型欠損した原虫でDGK1欠損原虫同様に赤血球侵入関連分子の表面分泌が阻害される知見を得た。その一方でAPH欠損原虫では赤血球侵入関連分子の細胞内局在の変化は見られなかった。これらの結果からDGK1によって転換されるPAとAPHの相互作用が赤血球侵入関連分子の分泌には必須だが、それら分子の細胞内局在はDGK1由来のPAのみが関与する可能性が示唆された。 |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Signaling mechanism of the apical organelle discharge by malaria parasites during erythrocyte invasion, 201904, 202203, 金子 修; 矢幡 一英; 石崎 隆弘; 麻田 正仁 |
| The Japan Science Society, Sasagawa Scientific Research Grant, マラリア原虫のタンパク質小胞輸送先を決定づける原虫側分子の探索, 202004, 202102 |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for JSPS Fellows, マラリア原虫の赤血球侵入関連分子分泌調節カスケードの解明, 20170426, 20200331, 石崎 隆弘, マラリア原虫の赤血球侵入は原虫の生存に必須なステップであり、宿主への病原性と密接な繋がりを持つ。赤血球から脱出した原虫はマイクロネームやロプトリーといった細胞小器官から種々の分子を順序立てて分泌しながら赤血球侵入を行う。マイクロネーム分子の分泌には細胞外液組成である低カリウム条件に起因するCa2+シグナルが関与することが明らかとなっているが、分子分泌に至るシグナル経路には不明な点が多い。本研究はネズミマラリア原虫を用いて、後期シゾント期における発現が高いキナーゼ群をDiCre-loxPシステムにより誘導型欠損させた原虫を使って、赤血球侵入に関わる標的キナーゼの同並びに、表現系の得られた分子の下流分子の探索を目的とする。 本年度は、得られたloxP挿入原虫もしくは遺伝子欠損原虫のうち、3つのキナーゼにおいて侵入能力が有意に減少するという結果が得られた。これら得られた3つのキナーゼのうち、2つのキナーゼは侵入能力、マウスへの病原性に対して顕著な減少が見られたことから赤血球侵入関連分子の分泌を免疫蛍光抗体法により評価した。その結果1つのキナーゼでは分子分泌のタイミングが遅れるということ、別のキナーゼでは侵入関連分子群の分泌そのものが低下するということが明らかとなった。これらの結果が引き起こされる分子メカニズムを明らかにするために、次年度はリン酸化プロテオーム解析及び、リン脂質メディエーターの探索を行う予定である。 |
Educational Activities
Teaching Experience
| 感染病理学特論(分担), Rakuno Gakuen University |
| 統合獣医学(分担), Rakuno Gakuen University |
| Fish Diseases, Rakuno Gakuen University |
| Practical lecture of infection and pathology, Rakuno Gakuen University |
| Practical training of Veterinary Parasitology, Rakuno Gakuen University |
| Veterinary Parasitology, Rakuno Gakuen University |
社会貢献活動情報
Social Contribution
| 2025 06 21 , 身近な感染症講座「蚊やマダニが媒介する病気とその制御法」, 酪農学園大学社会連携センター, 2025年度市民公開講座# |
| 2024 10 , ベクターが媒介する寄生虫・原虫病を知ろう！, 北海道獣医師会, 北海道ワンヘルスフェア2024# |
| 2024 08 17 , サイエンスファーム2024, 酪農学園大学獣医学群, 獣医大学が運営する高校生研究発表企画# |
| 2024 06 30 , WRA初級認定講座実習, 野生動物リハビリテーター協会, WRA初級認定講座# |
| 2024 06 22 , 愛護動物の概要を知り、皆さんの暮らしを豊かにしましょう, 酪農学園大学社会連携センター, 2024年度小学生・中学生・高校生向け講座# |
| 2021 09 , 東京大学医学部MD研究者育成プログラム 基礎医学ゼミ# |
| 2017 08 02 - 2017 08 03 , 平成29年度霞ヶ関子供見学デー, 文部科学省, 平成29年度霞ヶ関子供見学デー# |
| 2016 10 , スーパーグローバルハイスクール(長崎東高等学校)研究指導# |
| 2016 08 , World Mosquito Day 2016, 長崎大学フィラリアNTD室, World Mosquito Day 2016# |
| 2016 07 27 - 2016 07 28 , 平成28年度霞ヶ関子供見学デー, 文部科学省, 平成28年度霞ヶ関子供見学デー# |
教員総覧