School of Veterinary Medicine, Department of Veterinary Science
北澤多喜雄 キタザワ タキオ
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Name
KITAZAWA Takio
Affiliation (Master)
School of Veterinary Medicine, Department of Veterinary Science
Affiliation & Job
Rakuno Gakuen University School of Veterinary Medicine Department of Veterinary Science
Education
1979 04 - 1981 03 , Hokakido University Graduate School 1975 04 - 1979 03 , Hokkaido University, Veterinary Medicine
Association Memberships
The Japanese Society of Veterinary Nursing JAPAN SOCIETY OF SMOOTH MUSCLE RESEARCH THE JAPANESE PHARMACOLOGICAL SOCIETY THE JAPANESE SOCIETY OF VETERINARY SCIENCE
Committee Memberships
2020 08 - Today, Japan Pharmacological Society, Pharmacological Educator
Research Activities
Research Areas
Life sciences, Morphology, anatomy Life sciences, Veterinary medicine, Comparative Animal Pharmacology
Research Interests
musucarinic receptor, motilin, ghrelin, Comparative Biology, Gut hormone, Autonomic nerve, Gastrointestinal motility, Pharmacology,
Published Papers
Surface electromyography of the gluteus medius, vastus lateralis, and gastrocnemius muscles during sit-to-stand and stand-to-sit transitions in normal beagles., Kazuyuki Yoshikawa; Takio Kitazawa; Tadashi Sano; Takumi Ino; Tomoya Miyasaka; Kazuya Edamura, Research in Veterinary Science, 193, (105783) 1 - 5, 2025 06 Physiolgical roles of ghrelin in the regulation of gastrointestinal motility in vertebrates, Shuangyi Zhang; Hiroyuki Kaiya; Takio Kitazawa, General and Comparative Endocrinology, 365, (1468) 1 - 10, 2025 02 What is the function of motilin in the rabbit gastrointestinal tract?, Takio Kitazawa; Shuangyi Zhang, The Japanese Journal of Veterinary Research, 72, (3-4) 71 - 81, 2024 12 The Concise Guide to PHARMACOLOGY 2023/24: G protein-coupled receptors., Stephen P H Alexander; Arthur Christopoulos; Anthony P Davenport; Eamonn Kelly; Alistair A Mathie; John A Peters; Emma L Veale; Jane F Armstrong; Elena Faccenda; Simon D Harding; Jamie A Davies; Maria Pia Abbracchio; George Abraham; Alexander Agoulnik; Wayne Alexander; Khaled Al-Hosaini; Magnus Bäck; Jillian G Baker; Nicholas M Barnes; Ross Bathgate; Jean-Martin Beaulieu; Annette G Beck-Sickinger; Maik Behrens; Kenneth E Bernstein; Bernhard Bettler; Nigel J M Birdsall; Victoria Blaho; Francois Boulay; Corinne Bousquet; Hans Bräuner-Osborne; Geoffrey Burnstock; Girolamo Caló; Justo P Castaño; Kevin J Catt; Stefania Ceruti; Paul Chazot; Nan Chiang; Bice Chini; Jerold Chun; Antonia Cianciulli; Olivier Civelli; Lucie H Clapp; Réjean Couture; Helen M Cox; Zsolt Csaba; Claes Dahlgren; Gordon Dent; Steven D Douglas; Pascal Dournaud; Satoru Eguchi; Emanuel Escher; Edward J Filardo; Tung Fong; Marta Fumagalli; Raul R Gainetdinov; Michael L Garelja; Marc de Gasparo; Craig Gerard; Marvin Gershengorn; Fernand Gobeil; Theodore L Goodfriend; Cyril Goudet; Lukas Grätz; Karen J Gregory; Andrew L Gundlach; Jörg Hamann; Julien Hanson; Richard L Hauger; Debbie L Hay; Akos Heinemann; Deron Herr; Morley D Hollenberg; Nicholas D Holliday; Mastgugu Horiuchi; Daniel Hoyer; László Hunyady; Ahsan Husain; Adriaan P IJzerman; Tadashi Inagami; Kenneth A Jacobson; Robert T Jensen; Ralf Jockers; Deepa Jonnalagadda; Sadashiva Karnik; Klemens Kaupmann; Jacqueline Kemp; Charles Kennedy; Yasuyuki Kihara; Takio Kitazawa; Pawel Kozielewicz; Hans-Jürgen Kreienkamp; Jyrki P Kukkonen; Tobias Langenhan; Dan Larhammar; Katie Leach; Davide Lecca; John D Lee; Susan E Leeman; Jérôme Leprince; Xaria X Li; Stephen J Lolait; Amelie Lupp; Robyn Macrae; Janet Maguire; Davide Malfacini; Jean Mazella; Craig A McArdle; Shlomo Melmed; Martin C Michel; Laurence J Miller; Vincenzo Mitolo; Bernard Mouillac; Christa E Müller; Philip M Murphy; Jean-Louis Nahon; Tony Ngo; Xavier Norel; Duuamene Nyimanu; Anne-Marie O'Carroll; Stefan Offermanns; Maria Antonietta Panaro; Marc Parmentier; Roger G Pertwee; Jean-Philippe Pin; Eric R Prossnitz; Mark Quinn; Rithwik Ramachandran; Manisha Ray; Rainer K Reinscheid; Philippe Rondard; G Enrico Rovati; Chiara Ruzza; Gareth J Sanger; Torsten Schöneberg; Gunnar Schulte; Stefan Schulz; Deborah L Segaloff; Charles N Serhan; Khuraijam Dhanachandra Singh; Craig M Smith; Leigh A Stoddart; Yukihiko Sugimoto; Roger Summers; Valerie P Tan; David Thal; Walter Wally Thomas; Pieter B M W M Timmermans; Kalyan Tirupula; Lawrence Toll; Giovanni Tulipano; Hamiyet Unal; Thomas Unger; Celine Valant; Patrick Vanderheyden; David Vaudry; Hubert Vaudry; Jean-Pierre Vilardaga; Christopher S Walker; Ji Ming Wang; Donald T Ward; Hans-Jürgen Wester; Gary B Willars; Tom Lloyd Williams; Trent M Woodruff; Chengcan Yao; Richard D Ye, British journal of pharmacology, 180 Suppl 2, 2023 10 Does ghrelin regulate intestinal motility in rabbits? An in vitro study using isolated duodenal strips., Shuangyi Zhang; Hiroyuki Kaiya; Takio Kitazawa, General and comparative endocrinology, 344, 114384 - 114384, 2023 09 16 Kinematic characteristics of canine hindlimb movement during sit-to-stand and stand-to-sit motions., Kazuyuki Yoshikawa; Takio Kitazawa; Tadashi Sano; Takumi Ino; Tomoya Miyasaka, Research in veterinary science, 162, 104944 - 104944, 2023 06 30 Motilin is a regulator of gastric contraction in Japanese fire belly newts (Cynops pyrrhogaster), in vitro studies using isolated gastrointestinal strips of newts, rabbits, and chickens., Shuangyi Zhang; Hiroyuki Kaiya; Takio Kitazawa, General and comparative endocrinology, 330, 114140 - 114140, 2023 01 01 Metabarcoding of feces and intestinal contents to determine carnivorous diets in red-crowned cranes in eastern Hokkaido, Japan., Haruka Kataoka; Nanami Koita; Natsuko Ito Kondo; Hiroshi C Ito; Momoko Nakajima; Kunikazu Momose; Hiroko Iima; Tomoo Yoshino; Tomoko Amano; Takio Kitazawa; Daiji Endoh; Nobuyoshi Nakajima; Hiroki Teraoka, The Journal of veterinary medical science, 84, (3) 358 - 367, 2022 03 03 Pheasant motilin, its distribution and gastrointestinal contractility-stimulating action in the pheasant, Shuangyi Zhang; Hiroyuki Kaiya; Hiroki Teraoka; Takio Kitazawa, General and Comparative Endocrinology, Elsevier BV, 314, 113897 - 113897, 2021 12 THE CONCISE GUIDE TO PHARMACOLOGY 2021/22: G protein-coupled receptors., Stephen Ph Alexander; Arthur Christopoulos; Anthony P Davenport; Eamonn Kelly; Alistair Mathie; John A Peters; Emma L Veale; Jane F Armstrong; Elena Faccenda; Simon D Harding; Adam J Pawson; Christopher Southan; Jamie A Davies; Maria Pia Abbracchio; Wayne Alexander; Khaled Al-Hosaini; Magnus Bäck; Nicholas M Barnes; Ross Bathgate; Jean-Martin Beaulieu; Kenneth E Bernstein; Bernhard Bettler; Nigel J M Birdsall; Victoria Blaho; Francois Boulay; Corinne Bousquet; Hans Bräuner-Osborne; Geoffrey Burnstock; Girolamo Caló; Justo P Castaño; Kevin J Catt; Stefania Ceruti; Paul Chazot; Nan Chiang; Bice Chini; Jerold Chun; Antonia Cianciulli; Olivier Civelli; Lucie H Clapp; Réjean Couture; Zsolt Csaba; Claes Dahlgren; Gordon Dent; Khuraijam Dhanachandra Singh; Steven D Douglas; Pascal Dournaud; Satoru Eguchi; Emanuel Escher; Edward J Filardo; Tung Fong; Marta Fumagalli; Raul R Gainetdinov; Marc de Gasparo; Craig Gerard; Marvin Gershengorn; Fernand Gobeil; Theodore L Goodfriend; Cyril Goudet; Karen J Gregory; Andrew L Gundlach; Jörg Hamann; Julien Hanson; Richard L Hauger; Debbie L Hay; Akos Heinemann; Morley D Hollenberg; Nicholas D Holliday; Mastgugu Horiuchi; Daniel Hoyer; László Hunyady; Ahsan Husain; Adriaan P IJzerman; Tadashi Inagami; Kenneth A Jacobson; Robert T Jensen; Ralf Jockers; Deepa Jonnalagadda; Sadashiva Karnik; Klemens Kaupmann; Jacqueline Kemp; Charles Kennedy; Yasuyuki Kihara; Takio Kitazawa; Pawel Kozielewicz; Hans-Jürgen Kreienkamp; Jyrki P Kukkonen; Tobias Langenhan; Katie Leach; Davide Lecca; John D Lee; Susan E Leeman; Jérôme Leprince; Xaria X Li; Tom Lloyd Williams; Stephen J Lolait; Amelie Lupp; Robyn Macrae; Janet Maguire; Jean Mazella; Craig A McArdle; Shlomo Melmed; Martin C Michel; Laurence J Miller; Vincenzo Mitolo; Bernard Mouillac; Christa E Müller; Philip Murphy; Jean-Louis Nahon; Tony Ngo; Xavier Norel; Duuamene Nyimanu; Anne-Marie O'Carroll; Stefan Offermanns; Maria Antonietta Panaro; Marc Parmentier; Roger G Pertwee; Jean-Philippe Pin; Eric R Prossnitz; Mark Quinn; Rithwik Ramachandran; Manisha Ray; Rainer K Reinscheid; Philippe Rondard; G Enrico Rovati; Chiara Ruzza; Gareth J Sanger; Torsten Schöneberg; Gunnar Schulte; Stefan Schulz; Deborah L Segaloff; Charles N Serhan; Leigh A Stoddart; Yukihiko Sugimoto; Roger Summers; Valerie P Tan; David Thal; Walter Wally Thomas; Pieter B M W M Timmermans; Kalyan Tirupula; Giovanni Tulipano; Hamiyet Unal; Thomas Unger; Celine Valant; Patrick Vanderheyden; David Vaudry; Hubert Vaudry; Jean-Pierre Vilardaga; Christopher S Walker; Ji Ming Wang; Donald T Ward; Hans-Jürgen Wester; Gary B Willars; Trent M Woodruff; Chengcan Yao; Richard D Ye, British journal of pharmacology, 178 Suppl 1, 2021 10 Motilin Comparative Study: Structure, Distribution, Receptors, and Gastrointestinal Motility., Kitazawa Takio; Kaiya Hiroyuki, Frontier in Endocrinology, 12, (700884) 1 - 24, 2021 08 Functional assessment of the gluteus medius, cranial part of the biceps femoris, and vastus lateralis in Beagle dogs based on a novel gait phase classification., Kazuyuki Yoshikawa; Sae Tsubakishita; Tadashi Sano; Takumi Ino; Tomoya Miyasaka; Takio Kitazawa, The Journal of veterinary medical science, 83, (1) 116 - 124, 2021 01 23 Oxidative stress inducers potentiate 2,3,7,8-tetrachlorodibenzo-p-dioxin-mediated pre-cardiac edema in larval zebrafish, Katsuki TANAKA; Hikaru ADACHI; Hironobu AKASAKA; Junya TAMAOKI; Yuji FUSE; Makoto KOBAYASHI; Takio KITAZAWA; Hiroki TERAOKA, Journal of Veterinary Medical Science, Japanese Society of Veterinary Science, 83, (7) 1050 - 1058, 2021 Functions of Muscarinic Receptor Subtypes in Gastrointestinal Smooth Muscle: A Review of Studies with Rewceptor-Knockout Mice, Yasuyuki Tanahashi; Seiichi Komori; Hayato Matsuyama; Takio Kitazawa; Toshihiro Unno, Int. J. Mol. Sci. 2021, 22, 926., 22, (926) 1 - 24, 2021 01 Motilin- and ghrelin-induced contractions in isolated gastrointestinal strips from three species of frogs., Shuangyi Zhang; Hiroki Teraoka; Hiroyuki Kaiya; Takio Kitazawa, General and comparative endocrinology, 300, 113649 - 113649, 2021 01 01 Cytochrome P450 Expression and Chemical Metabolic Activity before Full Liver Development in Zebrafish., Tasuku Nawaji; Natsumi Yamashita; Haruka Umeda; Shuangyi Zhang; Naohiro Mizoguchi; Masanori Seki; Takio Kitazawa; Hiroki Teraoka, Pharmaceuticals (Basel, Switzerland), 13, (12) , 2020 12 11 Blood vessels are primary targets for 2,3,7,8-tetrachlorodibenzo-p-dioxin in pre-cardiac edema formation in larval zebrafish., Nijoukubo D, Adachi H, Kitazawa T, Teraoka H., Chemosphere., Elsevier BV, 254, (126808) 1 - 10, 2020 04 Identification of pheasant ghrelin and motilin and their actions on contractility of the isolated gastrointestinal tract.Identification of pheasant ghrelin and motilin and their actions on contractility of the isolated gastrointestinal tract., Zhang S; Okuhara Y; Iijima M; Takemi S; Sakata I; Kaiya H; Teraoka H; Kitazawa T, Gen Comp Endocrinol., 285, (1) 12 - 19, 2020 01 Genetic diversity of cytochrome P450 2A with different metabolic activities in domestic cats., Souta Sugiyama; Yasuhiro Uno; Tomoko Amano; Takio Kitazawa; Hiroki Teraoka, The Journal of veterinary medical science, 81, (7) 983 - 985, 2019 07 11 Genetic diversity of cytochrome P450 1A2 with different metabolic activities in domestic cats., Sugiyama S; Uno Y; Amano T; Kitazawa T; Teraoka H, J. Vte.Med. Sci, Japanese Society of Veterinary Science, 81, (7) 980 - 982, 2019 07 Limited expression of functional cytochrome p450 2c subtypes in the liver and small intestine of domestic cats., Ono Y; Sugiyama S; Matsushita M; Kitazawa T; Amano T; Uno Y; Ikushiro S; Teraoka H, Xenobiotica., Informa UK Limited, doi: 10.1080/00498254.2018.148, (6) 1 - 9, 2019 06 Transcription profiling of cytocrome P450 genes in the liver of adult zebrafish, Danio rerio., Kubota A; Kawai Y; Yamashita N; Lee JS; Kondoh D; Zhang S; Nishi Y; Suzuki K; Kitazawa T; Teraoka H, The Journal of Toxicological Sciences, (一社)日本毒性学会, 44, (Suppl.) S322 - S322, 2019 06 Regulation of Gastrointestinal Motility by Motilin and Ghrelin in Vertebrates, Kitazawa Takio; Kaiya Hiroyuki, Frontier in Endocrinology, doi: 10.3389/fendo.2019.00278, (MAY) 1 - 19, 2019 05 Genetic diversity of cytochrome P450 3A with different metabolic activity in domestic cats., Sugiyama S; Uno Y; Amano T; Kitazawa T; Teraoka H, J. Veterinary Medical Science, Japanese Society of Veterinary Science, 81, (4) 598 - 600, 2019 04 A verification study of gastrointestinal motility-stimulating action of guinea-pig motilin using isolated gastrointestinal strips from rabbits and guinea-pigs, Takio Kitazawa; Rio Harada; Ichiro Sakata; Takafumi Sakai; Hiroyuki Kaiya, General and Compaartive Endocrinology, 100, (1) 19 - 28, 2019 04 Alpha1B-adrenoceptor-mediated positive inotropic and positive chronotropic actions in the mouse atrium, Shuangyi Zhang Reia Takahashi; Natsumi Yamashita; Hiroki Teraoka; Takio Kitazawa, European J Pharmacology, 839, (1) 82 - 88, 2018 11 Contamination Status of Seven Elements in Hooded Cranes Wintering in South-West Kyushu, Japan: Comparison with Red-Crowned Cranes in Hokkaido, Japan, Hiroki Teraoka; Hasumi Miyagi; Yuko Haraguchi; Kozo Takase; Takio Kitazawa; Jun Noda, Archives of Environmental Contamination and Toxicology, Springer New York LLC, doi: 10.1007/s00244-018-0541-y, 1 - 9, 2018 05 31 Post-operative rehabilitation of a dog with tetraparesis resulting from cervical intervertebral disc herniation., Kitazawa Takio, Veterinary Nursing, 日本動物看護学会, 22, (2) 35 - 40, 2018 03 Aroclor 1254 and BDE-47 inhibit dopaminergic function manifesting as changes in locomotion behaviors in zebrafish embryos, Yasuaki Tanaka; Mari Fujiwara; Asako Shindo; Guojun Yin; Takio Kitazawa; Hiroki Teraoka, Chemosphere, Elsevier BV, 193, 1207 - 1215, 2018 02 Structural determination, distribution, and physiological actions of ghrelin in the guinea pig, Yuji Okuhara; Hiroyuki Kaiya; Hiroki Teraoka; Takio Kitazawa, Peptides, Elsevier Inc., 99, 70 - 81, 2018 01 01 Dexamethasone-induced hepatomegaly and steatosis in larval zebrafish, Guojun Yin; Liping Cao; Jinliang Du; Rui Jia; Takio Kitazawa; Akira Kubota; Hiroki Teraoka, JOURNAL OF TOXICOLOGICAL SCIENCES, 42, (4) 455 - 459, 2017 08 Does motilin peptide regulate gastrointestinal motility of zebrafish? An in vitro study using isolated intestinal strips, Takio Kitazawa; Maria Yoshida; Hiroki Teraoka; Hiroyuki Kaiya, GENERAL AND COMPARATIVE ENDOCRINOLOGY, 249, 15 - 23, 2017 08 The chicken is an interesting animal for study of the functional role of ghrelin in the gastrointestinal tract, Takio Kitazawa; Hiroki Teraoka; Hiroyuki Kaiya, ENDOCRINE JOURNAL, 64, S5 - S9, 2017 Characterization of feline cytochrome P450 2B6, Gaku Okamatsu; Tetsuya Komatsu; Yuka Ono; Hiroki Inoue; Tsuyoshi Uchide; Takenori Onaga; Daiji Endoh; Takio Kitazawa; Takeo Hiraga; Yasuhiro Uno; Hiroki Teraoka, XENOBIOTICA, 47, (2) 93 - 102, 2017 Functional expression and comparative characterization of four feline P450 cytochromes using fluorescent substrates, Gaku Okamatsu; Kei Kawakami; Tetsuya Komatsu; Takio Kitazawa; Yasuhiro Uno; Hiroki Teraoka, XENOBIOTICA, 47, (11) 951 - 961, 2017 Pharmacological characterization of muscarinic receptor subtypes involved in carbachol-induced contraction of the chicken proventriculus, Kitazawa Takio; Nahoko Kondo; Tetsuro Taneike, SM. J. Gastroenterol., 2, (1) 1003 - 1012, 2016 10 ムスカリン受容体を介した胃粘膜細胞の分化・増殖制御, 松山 勇人; 内藤 清惟; 和田 善明; 永野 宏; 齋藤 正一郎; 酒井 洋樹; 棚橋 靖行; 北澤 多喜雄; 小森 成一; 海野 年弘, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 159回, 497 - 497, 2016 08 Protective effect of prostacyclin against pre-cardiac edema caused by 2,3,7,8-tetrachlorodibenzo-p-dioxin and a thromboxane receptor agonist in developing zebrafish, Daisuke Nijoukubo; Yasuaki Tanaka; Yuki Okuno; Guojun Yin; Takio Kitazawa; Richard E. Peterson; Akira Kubota; Hiroki Teraoka, CHEMOSPHERE, 156, 111 - 117, 2016 08 Molecular cloning of motilin and mechanism of motilin-induced gastrointestinal motility in Japanese quail, Auvijit Saha Apu; Anupom Mondal; Takio Kitazawa; Shota Takemi; Takafumi Sakai; Ichiro Sakata, GENERAL AND COMPARATIVE ENDOCRINOLOGY, 233, 53 - 62, 2016 07 Effects of ghrelin and motilin on smooth muscle contractility of the isolated gastrointestinal tract from the bullfrog and Japanese fire belly newt, Takio Kitazawa; Misato Shimazaki; Ayumi Kikuta; Noriko Yaosaka; Hiroki Teraoka; Hiroyuki Kaiya, GENERAL AND COMPARATIVE ENDOCRINOLOGY, 232, 51 - 59, 2016 06 Regulation of heart contractility by M2 and M3 muscarinic receptors: Functional studies using muscarinic receptor knockout mouse, Takio Kitazawa; Hiroki Teraoka; Nao Harada; Kenta Ochi; Tatsuro Nakamura; Koichi Asakawa; Shinya Kanegae; Noriko Yaosaka; Toshihiro Unno; Sei Ichi Komori; Masahisa Yamada, Neuromethods, 107, 235 - 259, 2016 Dexamethasone-induced hepatomegaly and fatty liver in zebrafish, YIN Guojun; NIJOUKUBO Daisuke; KITAZAWA Takio; CAO Liping; DUB Jinliang; TERAOKA Hiroki, Annual Meeting of the Japanese Society of Toxicology, The Japanese Society of Toxicology, 43, P - 35, 2016 Accumulation properties of inorganic mercury and organic mercury in the red-crowned crane Grus japonensis in east Hokkaido, Japan, Hiroki Teraoka; Erika Okamoto; Moe Kudo; Shouta M. M. Nakayama; Yoshinori Ikenaka; Mayumi Ishizuka; Tetsuya Endo; Takio Kitazawa; Takeo Hiraga, ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 122, 557 - 564, 2015 12 Identification and functional characterization of novel feline cytochrome P450 2A, Gaku Okamatsu; Tetsuya Komatsu; Akira Kubota; Takenori Onaga; Tsuyoshi Uchide; Daiji Endo; Rikio Kirisawa; Guojun Yin; Hiroki Inoue; Takio Kitazawa; Yasuhiro Uno; Hiroki Teraoka, XENOBIOTICA, 45, (6) 503 - 510, 2015 06 Correlation of ghrelin concentration and ghrelin, ghrelin-O-acetyltransferase (GOAT) and growth hormone secretagogue receptor 1a mRNAs expression in the proventriculus and brain of the growing chicken, Takio Kitazawa; Takeo Hiraga; Hiroki Teraoka; Noriko Yaosaka; Hiroyuki Kaiya, PEPTIDES, 63, 134 - 142, 2015 01 What is the function of motilin in the rabbit gastrointestinal tract?, Takio Kitazawa; Shuangyi Zhang, The Japanese Journal of Veterinary Research, 72, (3-4) 71 - 81, 2014 12 Involvement of COX2-thromboxane pathway in TCDD-induced precardiac edema in developing zebrafish, Hiroki Teraoka; Yuki Okuno; Daisuke Nijoukubo; Ayumi Yamakoshi; Richard E. Peterson; John J. Stegeman; Takio Kitazawa; Takeo Hiraga; Akira Kubota, AQUATIC TOXICOLOGY, 154, 19 - 26, 2014 09 Roles of M-2 and M-3 muscarinic receptors in the generation of rhythmic motor activity in mouse small intestine, Y. Tanahashi; N. Waki; T. Unno; H. Matsuyama; S. Iino; T. Kitazawa; M. Yamada; S. Komori, NEUROGASTROENTEROLOGY AND MOTILITY, 25, (10) E687 - E697, 2013 10 Age-dependent reduction of ghrelin- and motilin-induced contractile activity in the chicken gastrointestinal tract, Takio Kitazawa; Akiko Yoshida; Takuya Tamano; Hiroki Teraoka; Hiroyuki Kaiya, Peptides, 43, 88 - 95, 2013 05 A ganglionic stimulant, 1,1-dimethyl-4-phenylpiperazinium, caused both cholinergic and adrenergic responses in the isolated mouse atrium, Kenta Ochi; Hiroki Teraoka; Toshihiro Unno; Sei-Ichi Komori; Masahisa Yamada; Takio Kitazawa, European Journal of Pharmacology, 704, (1-3) 7 - 14, 2013 03 15 Large-Scale Survey of Mitochondrial D-Loop of the Red-Crowned Crane Grus japonensis in Hokkaido, Japan by Convenient Genotyping Method, Yoshiaki Miura; Akira Shiomi; Junya Shiraishi; Kohei Makita; Mitsuhiko Asakawa; Takio Kitazawa; Takeo Hiraga; Yulia Momose; Kunikazu Momose; Hiroyuki Masatomi; Hiroki Teraoka, JOURNAL OF VETERINARY MEDICAL SCIENCE, 75, (1) 43 - 47, 2013 01 Evidence for M2 and M3 Muscarinic receptor involvement in cholinergic excitatory junction potentials through synergistic activation of Cation channels in the longitudinal muscle of mouse ileum, Hayato Matsuyama; Yasuyuki Tanahashi; Takio Kitazawa; Masahisa Yamada; Seiichi Komori; Toshihiro Unno, Journal of Pharmacological Sciences, 121, (3) 227 - 236, 2013 Origin of three red-crowned cranes Grus japonensis found in northeast Honshu and West Hokkaido, Japan, from 2008 to 2012, Yoshiaki Miura; Junya Shiraishi; Akira Shiomi; Takio Kitazawa; Takeo Hiraga; Fumio Matsumoto; Hiroki Teraoka; Hiroyuki Masatomi, Journal of Veterinary Medical Science, 75, (9) 1241 - 1244, 2013 Immunohistochemical and functional studies for M 3 muscarinic receptors and cyclo-oxygenase-2 expressed in the mouse atrium, N. Harada; K. Ochi; N. Yaosaka; H. Teraoka; T. Hiraga; T. Iwanaga; T. Unno; S. Komori; M. Yamada; T. Kitazawa, Autonomic and Autacoid Pharmacology, 32, (3) 41 - 52, 2012 10 Ghrelin does not affect gastrointestinal contractility in rainbow trout and goldfish in vitro, Takio Kitazawa; Kentaro Itoh; Noriko Yaosaka; Keisuke Maruyama; Kouhei Matsuda; Hiroki Teraoka; Hiroyuki Kaiya, GENERAL AND COMPARATIVE ENDOCRINOLOGY, 178, (3) 539 - 545, 2012 09 Changes of Mercury Contamination in Red-Crowned Cranes, Grus japonensis, in East Hokkaido, Japan, Hiroki Teraoka; Yukari Tagami; Moe Kudo; Yoshiaki Miura; Erika Okamoto; Fumio Matsumoto; Kimiya Koga; Akiko Uebayashi; Ryoji Shimura; Masako Inoue; Kunikazu Momose; Hiroyuki Masatomi; Takio Kitazawa; Takeo Hiraga; Annamalai Subramanian, ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 63, (1) 153 - 160, 2012 07 Differential Display System with Vertebrate-Common Degenerate Oligonucleotide Primers: Uncovering Genes Responsive to Dioxin in Avian Embryonic Liver, Hiroki Teraoka; Shino Ito; Haruki Ikeda; Akira Kubota; M. M. Abou Elmagd; Takio Kitazawa; Eun-Young Kim; Hisato Iwata; Daiji Endoh, ENVIRONMENTAL SCIENCE & TECHNOLOGY, 46, (1) 27 - 33, 2012 01 Expression of Two Novel Cytochrome P450 3A131 and 3A132 in Liver and Small Intestine of Domestic Cats, Kouichi Honda; Tetsuya Komatsu; Fumika Koyama; Akira Kubota; Kei Kawakami; Hiroyuki Asakura; Yasuhiro Uno; Takio Kitazawa; Takeo Hiraga; Hiroki Teraoka, JOURNAL OF VETERINARY MEDICAL SCIENCE, 73, (11) 1489 - 1492, 2011 11 Muscarinic receptor subtypes involved in regulation of colonic motility in mice: Functional studies using muscarinic receptor-deficient mice, Takaji Kondo; Miwa Nakajima; Hiroki Teraoka; Toshihiro Unno; Sei-ichi Komori; Masahisa Yamada; Takio Kitazawa, EUROPEAN JOURNAL OF PHARMACOLOGY, 670, (1) 236 - 243, 2011 11 Molecular identification of ghrelin receptor (GHS-R1a) and its functional role in the gastrointestinal tract of the guinea-pig, Takio Kitazawa; Tatsuro Nakamura; Atsuki Saeki; Hiroki Teraoka; Takeo Hiraga; Hiroyuki Kaiya, PEPTIDES, 32, (9) 1876 - 1886, 2011 09 Colon-specific contractile responses to tetrodotoxin in the isolated mouse gastrointestinal tract, Y. Okuno; T. Kondo; A. Saeki; E. Uchida; H. Teraoka; T. Kitazawa, Autonomic and Autacoid Pharmacology, 31, (1-2) 21 - 30, 2011 01 Molecular Cloning and Expression of Cytochrome P450 2D6 in the Livers of Domestic Cats, Tetsuya Komatsu; Kouichi Honda; Akira Kubota; Takio Kitazawa; Takeo Hiraga; Hiroki Teraoka, JOURNAL OF VETERINARY MEDICAL SCIENCE, 72, (12) 1633 - 1636, 2010 12 Ghrelin stimulates gastric motility of the guinea pig through activation of a capsaicin-sensitive neural pathway: in vivo and in vitro functional studies, T. Nakamura; T. Onaga; T. Kitazawa, NEUROGASTROENTEROLOGY AND MOTILITY, 22, (4) 446 - +, 2010 04 Muscarinic Receptor Subtypes Mediating Ca2+ Sensitization of Intestinal Smooth Muscle Contraction: Studies with Receptor Knockout Mice, Mayuko Suguro; Hayato Matsuyama; Yasuyuki Tanahashi; Toshihiro Unno; Takio Kitazawa; Masahisa Yamada; Seiichi Komori, JOURNAL OF VETERINARY MEDICAL SCIENCE, 72, (4) 443 - 451, 2010 04 Molecular cloning of growth hormone secretagogue-receptor and effect of quail ghrelin on gastrointestinal motility in Japanese quail, Takio Kitazawa; Yoshimi Maeda; Hiroyuki Kaiya, REGULATORY PEPTIDES, 158, (1-3) 132 - 142, 2009 11 M-3 Muscarinic Receptors Mediate Positive Inotropic Responses in Mouse Atria: A Study with Muscarinic Receptor Knockout Mice, Takio Kitazawa; Koichi Asakawa; Tatsuro Nakamura; Hiroki Teraoka; Toshihiro Unno; Sei-ichi Komori; Masahisa Yamada; Juergen Wess, JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 330, (2) 487 - 493, 2009 08 A non-selective cationic channel activated by diacylglycerol in mouse intestinal myocytes, Takashi Sakamoto; Hayato Matsuyama; Masayuki Yamamoto; Yasuyuki Tanahashi; Takio Kitazawa; Tetsuro Taneike; Seiichi Komori; Toshihiro Unno, EUROPEAN JOURNAL OF PHARMACOLOGY, 599, (1-3) 54 - 57, 2008 12 Excitatory and inhibitory 5-hydroxytryptamine (5-HT) receptors expressed in the isolated porcine uterine muscles, Tatsuro Nakamura; Takio Kitazawa; Jinshan Cao; Takuya Iwamoto; Hiroki Teraoka; Koichi Kadota; Tetsuro Taneike, EUROPEAN JOURNAL OF PHARMACOLOGY, 600, (1-3) 123 - 129, 2008 12 Muscarinic receptor subtypes involved in carbachol-induced contraction of mouse uterine smooth muscle, Takio Kitazawa; Ryuichi Hirama; Kozue Masunaga; Tatsuro Nakamura; Koichi Asakawa; Jinshan Cao; Hiroki Teraoka; Toshihiro Unno; Sei-ichi Komori; Masahisa Yamada; Juergen Wess; Tetsuro Taneike, NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 377, (4-6) 503 - 513, 2008 06 Characterization of prostanoid receptors present on adrenergic neurons innervating the porcine uterine longitudinal muscle, Jinshan Cao; Tatsuro Nakamura; Takio Kitazawa; Naoko Yamashiki; Tsubasa Yamamoto; Tetsuro Taneike, PROSTAGLANDINS & OTHER LIPID MEDIATORS, 86, (1-4) 26 - 34, 2008 06 高齢犬におけるトラマドールの薬物動態, 内田 陽介; 北澤 多喜雄; 井上 博紀; 山下 和人, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 144回, 152 - 152, 2007 08 Three distinct muscarinic signalling pathways for cationic channel activation in mouse gut smooth muscle cells, Takashi Sakamoto; Toshihiro Unno; Takio Kitazawa; Tetsuro Taneike; Masahisa Yamada; Jurgen Wess; Masakazu Nishimura; Seiichi Komori, JOURNAL OF PHYSIOLOGY-LONDON, 582, (1) 41 - 61, 2007 07 Contractile effects of ghrelin-related peptides on the chicken gastrointestinal tract in vitro, Takio Kitazawa; Hiroyuki Kaiya; Tetsuro Taneike, PEPTIDES, 28, (3) 617 - 624, 2007 03 Functional roles of muscarinic M-2 and M-3 receptors in mouse stomach motility: Studies with muscarinic receptor knockout mice, Takio Kitazawa; Kano Hashiba; Jinshan Cao; Toshihiro Unno; Sei-ichi Komori; Masahisa Yamada; Jurgen Wess; Tetsuro Taneike, EUROPEAN JOURNAL OF PHARMACOLOGY, 554, (2-3) 212 - 222, 2007 01 Endogenous prostaglandins regulate spontaneous contractile activity of uterine strips isolated from non-pregnant pigs, Jinshan Cao; Takio Kitazawa; Kazushige Takehana; Tetsuro Taneike, PROSTAGLANDINS & OTHER LIPID MEDIATORS, 81, (3-4) 93 - 105, 2006 12 Pharmacological characterization of 5-hydroxytryptamine-induced contraction in the chicken gastrointestinal tract, T. Kitazawa; H. Ukai; S. Komori; T. Taneike, Autonomic and Autacoid Pharmacology, 26, (2) 157 - 168, 2006 Intracerebroventricular Administration of Growth Hormone Releasing Peptide-6 (GHRP-6) Inhibits Food Intake, but not Food Retention of Crop and Stomaeh in Neonatal Chicks, Md. Sakirul Islam Khan; Koh-Ichi Dodo; Kazuki Yahata; Sayuri Nishimoto; Hiroshi Ueda; Tetsuro Taneike; Takio Kitazawa; Yoshinao Hosaka; Takashi Bungo, Journal of Poultry Science, 43, (1) 35 - 40, 2006 An in Vitro Study on Spontaneous Contractility and Drug Responsiveness of Porcine Myometrium During Three Ovarian Stages, KITAZAWA Takio; MIYAZAKI Reiko; ISOE Gentaro; CAO Jinshan; TANEIKE Tetsuro, The Japanese Journal of Swine Husbandry Research, The Japanese Society of Swine Science, 42, (4) 165 - 177, 2005 12 26 Gastric motor effects of peptide and non-peptide ghrelin agonists in mice in vivo and in vitro, T Kitazawa; B De Smet; K Verbeke; Depoortere, I; TL Peeters, GUT, 54, (8) 1078 - 1084, 2005 08 Uterine region-dependent differences in responsiveness to prostaglandins in the non-pregnant porcine myometrium, JS Cao; M Yosida; T Kitazawa; T Taneike, PROSTAGLANDINS & OTHER LIPID MEDIATORS, 75, (1-4) 105 - 122, 2005 01 Intracerebroventricular administration of chicken motilin does not induce hyperphagia in meat-type chicks, T Bungo; H Ueda; T Kitazawa; T Taneike; M Furuse, PHYSIOLOGY & BEHAVIOR, 82, (2-3) 199 - 203, 2004 09 Thromboxane A(2) (TP) receptor in the non-pregnant porcine myometrium and its role in regulation of spontaneous contractile activity, JS Cao; A Wakatsuki; M Yoshida; T Kitazawa; T Taneike, EUROPEAN JOURNAL OF PHARMACOLOGY, 485, (1-3) 317 - 327, 2004 02 Pregnancy-associated changes in responsiveness of the porcine myometrium to bioactive substances, T Kitazawa; H Hatakeyama; JS Cao; T Taneike, EUROPEAN JOURNAL OF PHARMACOLOGY, 469, (1-3) 135 - 144, 2003 05 5-HT7 receptor-mediated relaxation of the oviduct in nonpregnant proestrus pigs, M Inoue; T Kitazawa; J Cao; T Taneike, EUROPEAN JOURNAL OF PHARMACOLOGY, 461, (2-3) 207 - 218, 2003 02 In vitro pharmacological characterization of the prostanoid receptor population in the non-pregnant porcine myometrium, JS Cao; M Shayibuzhati; T Tajima; T Kitazawa; T Taneike, EUROPEAN JOURNAL OF PHARMACOLOGY, 442, (1-2) 115 - 123, 2002 05 5-HT7 receptor and beta(2)-adrenoceptor share in the inhibition of porcine uterine contractility in a muscle layer-dependent manner, T Kitazawa; K Nakagoshi; H Teraoka; T Taneike, EUROPEAN JOURNAL OF PHARMACOLOGY, 433, (2-3) 187 - 197, 2001 12 Muscle layer- and region-dependent distributions of oxytocin receptors in the porcine myometrium, T Kitazawa; T Kajiwara; A Kiuchi; H Hatakeyama; T Taneike, PEPTIDES, 22, (6) 963 - 974, 2001 06 Change of the involvement of 5-HT3 receptor in the gastric motor stimulating actions of KW-5139 (Leu(13)-motilin acetate) in the recovered and post-operative periods in dogs, T Yokoyama; K Takasaki; T Kitazawa; A Karasawa; H Kusaka, LIFE SCIENCES, 67, (19) 2281 - 2292, 2000 09 Smooth muscle layer-dependent distribution of 5-hydroxytryptamine(7) receptor in the porcine myometrium, T Kitazawa; Y Yamada; H Iwano; H Yokota; A Yuasa; T Taneike, BRITISH JOURNAL OF PHARMACOLOGY, 130, (1) 79 - 89, 2000 05 Characterization of functional endothelin receptors in the porcine myometrium, M Isaka; K Takaoka; Y Yamada; Y Abe; T Kitazawa; T Taneike, PEPTIDES, 21, (4) 543 - 551, 2000 04 The mechanisms of alpha(2)-adrenoceptor agonist-induced contraction in longitudinal muscle of the porcine uterus, T Kitazawa; Y Maezono; T Taneike, EUROPEAN JOURNAL OF PHARMACOLOGY, 390, (1-2) 185 - 195, 2000 02 5-Hydroxytryptamineのブタ子宮筋収縮抑制作用とその細胞内メカニズム, 北澤 多喜雄; 山田 裕子; 宮庄 拓; 岩野 英知; 横田 博; 湯浅 亮; 種池 哲朗, 獣医生化学, 37, 39 - 50, 2000 Mechanisms of 5-hydroxytryptamine-induced inhibition in the porcine myometrium, T Kitazawa; K Takaoka; T Taneike, JOURNAL OF AUTONOMIC PHARMACOLOGY, 19, (2) 65 - 75, 1999 04 Characterization of the muscarinic receptor subtype that mediates the contractile response of acetylcholine in the swine myometrium, T Kitazawa; F Uchiyama; K Hirose; T Taneike, EUROPEAN JOURNAL OF PHARMACOLOGY, 367, (2-3) 325 - 334, 1999 02 Smooth muscle layer-specific variations in the autonomic innervation of bovine myometrium, T Taneike; T Kitazawa; H Funakura; K Asanuma; S Tsuji; M Yamada; H Teraoka; A Ohga, GENERAL PHARMACOLOGY, 32, (1) 91 - 100, 1999 01 Involvement of 5-hydroxytryptamine(7) receptors in inhibition of porcine myometrial contractility by 5-hydroxytryptamine, T Kitazawa; O Kubo; M Satoh; T Taneike, BRITISH JOURNAL OF PHARMACOLOGY, 123, (2) 173 - 182, 1998 01 Functional characterization of neural and smooth muscle motilin receptors in the chicken proventriculus and ileum, T Kitazawa; T Taneike; A Ohga, REGULATORY PEPTIDES, 71, (2) 87 - 95, 1997 08 In vitro and in vivo investigations of the effect of Leu13-porcine motilin on pig gastrointestinal motility, T Kitazawa; S Kikui; T Taneike; A Ohga; K Sugisaki; N Tomizawa; S Hara, DIGESTIVE PHYSIOLOGY IN PIGS, (88) 158 - 162, 1997 Does motilin stimulate the gastrointestinal motility of the pig? In vitro study using smooth muscle strips and dispersed muscle cells, T Kitazawa; S Kikui; T Taneike; A Ohaga, GENERAL PHARMACOLOGY, 27, (4) 655 - 664, 1996 06 RECOVERY OF GASTROINTESTINAL MOTILITY FROM POSTOPERATIVE ILEUS IN DOGS - EFFECTS OF LEU(13)-MOTILIN (KW-5139) AND PROSTAGLANDIN-F2-ALPHA, T YOKOYAMA; T KITAZAWA; K TAKASAKI; A ISHII; A KARASAWA, NEUROGASTROENTEROLOGY AND MOTILITY, 7, (4) 199 - 210, 1995 12 BINDING AND FUNCTIONAL-CHARACTERIZATION OF ALPHA-2-ADRENOCEPTORS IN ISOLATED SWINE MYOMETRIUM, T TANEIKE; T NARITA; T KITAZAWA; S BANDO; H TERAOKA; A OHGA, JOURNAL OF AUTONOMIC PHARMACOLOGY, 15, (2) 93 - 105, 1995 04 MUSCLE LAYER AND REGIONAL DIFFERENCES IN AUTONOMIC INNERVATION AND RESPONSIVENESS TO TRANSMITTER AGENTS IN SWINE MYOMETRIUM, T TANEIKE; SI BANDO; K TAKASAKI; M OKUMURA; H SATO; H TERAOKA; T KITAZAWA; A OHGA, JOURNAL OF AUTONOMIC PHARMACOLOGY, 14, (3) 213 - 227, 1994 06 Enhancement by KW-5092, a Novel Gastroprokinetic Agent, of the Gastrointestinal Motor Activity in Dogs., Kishibayashi Nobuyuki; Tomaru Atsushi; Ichikawa Shunji; Kitazawa Takio; Shuto Katsuichi; Ishii Akio; Karasawa Akira, Journal of Pharmacological Sciences, The Japanese Pharmacological Society, 65, (2) 131 - 142, 1994 Antagonist like action of synthetic α2 -adrenoceptor agonists on contractile response to catecholamines in smooth muscle strips isolated from rainbow trout stomach (Salmo gairdneri), Kitazawa Takio; Miyashita Naoki; Chugun Akihito; Temma Kyosuke; Kondo Hiroshi, Comparative Biochemistry and Physiology. Part C, Comparative, 91, (2) 585 - 588, 1988
Books etc
Muscarinic regulation of gastrointestinal motility, Tanahashi Yasuyuki; Takio Kitazawa; Toshihiro Unno, Muscarinic receptor From structure to animal models Second edition. 307-339p, Humana Press registered company Springer Science+Business Media, LLC, part of Springer Nature., 2024 07 , 9781071640159 動物病理学 : 動物薬理学, 日本動物保健看護系大学協会カリキュラム委員会, 動物薬理学 2章の5,6節、3章と4章, インターズー, 2020 01 20 , 9784866710860 心臓収縮調節に関わる自律神経受容体の再検討―マウス心房筋を用いての解析ー, 北澤多喜雄, MPアグロ, 2019 04 伴侶動物におけるグレリン研究ー基礎とその臨床応用ー, 北澤多喜雄, 生物科学 69（２）, 生物科学 69（２）農文協, 2018 02 Regulation of heart contractility by M2 and M3 muscarinic receptors: functional studies using muscarinic receptor knockout mouse., Kitazawa T; Teraoka H; Harada N; Ochi K; Nakamura T; Asakawa K; Kanegae S; Yaosaka N; Unno T; Komori S; Yamada M, Chapter 13, Muscarinic receptor. From Structure to Animal Models. Neuromethods Vol. 107. DOI 10.1007/978-1-4939-2858-3_13@Springer Science+Business Media New York. 2015. 235-259, 2015 08 動物薬理学, 北澤多喜雄, 2,3,4,11章, Interzoo, 2014 10 獣医薬理学, 北澤多喜雄, 第13章 ホルモン, 近代出版, 2013 03 薬理学・毒性学実験, 北澤多喜雄, 25-29P, 117-118P, 文英堂, 2008 04
Conference Activities & Talks
Comparative study for regulation of gastrointestinal motiity by motilin, Takio Kitazawa, 156 Annual meeting for Japan veterinary science meeting 北海道東部に生息するタンチョウの糞および腸管内容 物のメタバーコーディング解析：動物食性の検討, 小板 ななみ; 片岡 陽香; 北澤 多喜雄; 飯間 裕子; 吉野 智生; 百瀬 邦和; 中嶋 信美; 遠藤 大二; 寺岡 宏樹, 第163回日本獣医学会学術集会 ネコにおける異物代謝に関わるシトクローム P450分子種の特徴, 尾野 由佳; 岡松 岳; 杉山 颯太; 宇野 泰広; 生城 真一; 北澤 多喜雄; 寺岡 宏樹, 第163回日本獣医学会学術集会 Potentiation of Dioxin-Induced Edema by LPS in Larval Zebrafish, H. Adachi; K. Tanaka; H. Kitamura; T. Kitazawa; H. Teraoka, Society of Toxicology 59th Annual Meeting ゼブラフィッシュ初期発生における肝臓形成前の化学物質代謝, 赤坂 洪暢; 縄司 奨; 山下夏未; 溝口直洋; 安達真里花; 関 雅範; Zhang Shuangy; 北澤多喜雄; 寺岡 宏樹, 野生動物に対するケミカルハザードに関する国際シンポジウム Cytochrome P450s expression and chemical metabolic activity before liver formation in zebrafish eleutheroembryos, 縄司奨; 山下夏未; 溝口直洋; 安達真里花; 関雅範; 北澤多喜雄; 寺岡宏樹, 第42回日本分子生物学会年会 Does motilin regulate gastrointestinal motility of bullfrog? In vitro study uisng isolated GI strips., Kitazawa Takio; Shuangyi Zhang; Teraoka Hiroki; Okuhara Yuji; Kaiya Hiroyuki, NeuroGastro 2019 (Lisobon) Identification of ghrelin and motilin in the pheasant , and their mechanical actions in the isolated gastrointestinal tract., Shuangyi Zhang; Ichiro Sakata; Hiroyuki Kaiya; HirokiTeraoka; Yuji Okuhara; Takio Kitazawa, NeuroGastro 2019 Transcriptional profiling of cytochrome P450 genes in adult liver and in whole embryo fry of zebrafish in some developmental stages, Hiroki Teraoka; Natsumi Yamashita; Yusuke K. Kawai; Shuangyi Zhang; Takio Kitazawa; Akira Kubota, 15th International Congress of Toxicology ゼブラフィッシュ肝臓におけるシトクロムP450分子種のmRNA発現プロファイル, 久保田彰; 川合佑典; 山下夏未; Jae Seung Lee; 近藤大輔; Shuangyi Zhang, 第46回 日本毒性学会学術年会 Alpha1B-adrenoceptor-mediated positive inotropic and positive chronotropic actions in the mouse atrium, Zhang S; Takahashi R; Yamashita N; Teraoka H; Kitazawa T, 92nd Annual Meeting of Japan Pharmacological Society A review of the motilin system in guinea-pig: Re-examination of gastrointestinal motility-stimulating action of guinea-pig motilin candidate in vitro, Kitazawa Takio; Hiroyuki Kaiya, 3rd meeting of the federation of neurogastroenterology and motility Guinea-pig ghrelin, its structure, distribution and function in gastrointestinal trac, Takio Kitazawa; Yuji Okuhara; Hiroyuki Kaiya; Hiroki Teraoka, World Clinical Pharmacology 2018 Kyoto Effects of prostaglandin E2 and F2 α on expression of growth factors and pro-, Shuangyi Zhang; Kitazawa Takio; Jinshan Cao, World Clinical Pharmacology 2018 Kyoto ダイオキシン毒性と炎症のクロストーク, 田中勝貴; 二条久保大祐; 安達 光; 北澤多喜雄; 寺岡宏樹, 2017年度生命科学系学会合同年次大会（ConBio2017） 犬血漿中アミノ酸量のバランス変化による腫瘍診断法の確立, 猿渡菜美香; 番目北澤多, 第16回日本獣医学会学術集会 発達期ゼブラフィッシュにおけるPCBおよび臭素化難燃物による自発運動の増強：ドーパミン神経経路とメラニン合成経路の関与, 田中泰彰; 藤原真理; 北澤多喜雄; 寺岡宏樹, 第44回日本毒性学会学術年会 Chicken is a unique animal model for studying function of ghrelin in gastrointestinal motility, Kitazawa Takio, International Symposium on Ghrelin and Energy metabolism Homeostasis Vascular Endothelium Is A Target Of 2,3,7,8-tetrachlorodibenzo-p-dioxin In Cardiac Edema In Early Zebrafish Larvae, 二条久保大祐; 田中勝貴; 田中泰彰; 北澤多喜雄; 寺岡宏樹, 第56回全米トキシコロジー学会 ムスカリン受容体を介した胃粘膜細胞の分化・増殖制御, 松山勇人; 内藤清惟; 和田善明; 永野宏; 斉藤正一郎; 酒井洋樹; 棚橋靖行; 北澤多喜雄; 小森成一; 海野年弘, 第159回日本獣医学会学術集会 Possible regulation of gastrointestinal motility of zebrafish by motilin: An invitro study using isolated muscle strips, Kitazawa Takio, RegPep2016 What is the general action of ghrelin invertebrates? Study focused on gastrointestinal motor stimulating action, Kitazawa Takio, 第93回日本生理学会
Works
脊髄のカテコールアミン反応性, 北澤多喜雄, 1979 04 - 1981 03 , 北海道大学大学院修士課程での仕事
修士論文の内容は以下に公表した。
Effects of catecholamines on spinal motoneurones and spinal reflex discharges in the isolated spinal cord of the newborn rat.
Kitazawa T, Saito K, Ohga A.
Brain Res. 1985 Mar;351(1):31-6.
Misc
Does oxytocin directly regulate the atrial contractility in mouse?, Makoto Ito; Takio Kitazawa, J.Rakuno Gakuen Univ., 49, (2) 101 - 109, 2025 03 Is motilin a physiological regulator of rabbit gastrointestinal motility?, Kitazawa Takio; Zhang Shuangyi, J.Rakuno Gakuen Univ., 49, (2) 81 - 99, 2025 03 Physiological roles of muscarinic acetylcholine receptor in the gastrointestinal tract: Functional study using muscarinic receptor knockout mice, Kitazawa Takio, . Rakuno Gakuen Univ., 48 (2)：255～276 (2024), 48, (2) 255 - 276, 2024 03 Comparative study for motilin in vertebrates; its structure, distribution, receptor and gastrointestinal motility stimulating action, Takio Kitazawa; Shuangyi Zhang; Hiroyuki Kaiya, J. Rakuno Gakuen Univ.,, 47, (2) 149 - 177, 2023 03 Ghrelin in vertebrates; distribution, receptor, release mechanism and regulation of gastrointestinal motility, Takio KITAZAWA; Shuangyi ZHANG; Hiroyuki KAIYA, J. Rakuno Gakuen Univ., 47 (2)：179～197 (2023), 47, (2) 179 - 197, 2023 03 Smooth muscle layer- and region-dependent differences in autonomic innervation and responsiveness to bioactive substances in the porcine uterus, Takio KITAZAWA; Tetsuro TANEIKE; Jinshan CAO, J. Rakuno Gakuen Univ.,, 46, (2) 15 - 27, 2022 03 10 Regulation of cardiac contractility by autonomic nerves and their neurotransmitters in the isolated mouse atrium, 北 澤 多喜雄; 浅 川 幸 一; 原 田 奈 央; 越 智 建 太; 高 橋 礼 奈; Zhang SHUANGYI, J. Rakuno Gakuen Univ.,, 46, (2) 1 - 13, 2022 03 10 ネコの膀胱炎から分離された多剤耐性大腸菌に対する新規ファージの分離とその性状解析, 大橋春香; 大橋春香; 中村暢宏; 酒井俊和; 藤木純平; 北澤多喜雄; 村田亮; 岩崎智仁; 岩野英知, 日本獣医学会学術集会講演要旨集, 164th (CD-ROM), 2021 発達期ゼブラフィッシュのAHR2介在性浮腫における炎症および酸化ストレスの関与, 田中 勝貴; 安達 光; 二条久保 大祐; 北澤 多喜雄; 寺岡 宏樹, The Journal of Toxicological Sciences, 43, (Suppl.) S327 - S327, 2018 06 ダイオキシン毒性と炎症のクロストーク, 田中 勝貴; 二条久保 大祐; 安達 光; 北澤 多喜雄; 寺岡 宏樹, 生命科学系学会合同年次大会, 2017年度, 2017 12 犬血漿中アミノ酸量のバランス変化による腫瘍診断法の確立, 猿渡 菜美香; 樋口 豪紀; 北澤 多喜雄; 福本 真也; 藤木 純平; 打出 毅; 遠藤 能史; 廉澤 剛; 井上 博紀; 岩野 英知, 日本獣医学会学術集会講演要旨集, 160回, 2017 08 マウス結腸輪走筋のコリン作動性神経-平滑筋伝達におけるM2およびM3ムスカリン受容体サブタイプの役割, 松山 勇人; 佐久間 絵光; 永野 宏; 棚橋 靖行; 北澤 多喜雄; 海野 年弘, 日本獣医学会学術集会講演要旨集, 160回, 2017 08 動物看護学課程で提供される望ましい野生動物(医)学教育とは―酪農学園大学獣医学群における事例から―, 浅川満彦; 郡山尚紀; 佐野忠士; 北澤多喜雄, Veterinary Nursing, 22, (1) 43 - 43, 2017 07 01 イヌ血液中アミノ酸量の変化に基づいた腫瘍診断法の確立, 猿渡 菜美香; 大野 さや; 北澤 多喜雄; 井上 博紀; 福本 真也; 打出 毅; 遠藤 能史; 廉澤 剛; 岩野 英知, Veterinary Nursing, 22, (1) 29 - 29, 2017 07 発達期ゼフラフィッシュにおけるPCBおよび臭素化難燃物による自発運動の増強 ドーパミン神経経路とメラニン合成経路の関与, 田中 泰彰; 藤原 麻里; 北澤 多喜雄; 寺岡 宏樹, The Journal of Toxicological Sciences, 42, (Suppl.) S282 - S282, 2017 06 犬血漿中アミノ酸量のバランス変化による腫瘍診断法の確立, 岩野英知; 猿渡菜美香; 大野さや; 樋口豪紀; 北澤多喜雄; 福本真也; 打出毅; 遠藤能史; 廉澤剛; 横田博, 北海道獣医師会雑誌, 60, (8) 406 - 406, 2016 08 10 タンチョウの水銀蓄積特性に関する検討, 寺岡宏樹; 岡本絵梨佳; 久道萌; 中山翔太; 池中良徳; 石塚真由美; 北澤多喜雄, 環境化学討論会要旨集(CD-ROM), 24th, 2015 06 17 ネコ、イヌ、ヒトにおけるシトクロームP450 3A分子種の動物種差, 岡松 岳; 川上 圭; 小松 徹也; 宇野 泰広; 北澤 多喜雄; 寺岡 宏樹, 中毒研究, 27, (3) 279 - 279, 2014 09 CHICKEN IS A SPECIFIC AND USEFUL ANIMAL MODEL TO STUDY THE FUNCTIONAL ROLE OF GHRELIN AND MOTILIN IN REGULATION OF GASTROINTESTINAL MOTILITY, T. Kitazawa; H. Teraoka; H. Kaiya, JOURNAL OF MOLECULAR NEUROSCIENCE, 53, 2014 08 Involvement of M-2 or M-3 muscarinic receptor-coupled signalling molecules in the activation of muscarinic cation channels in mouse ileal smooth muscle cells, Toshihiro Unno; Taisuke Katsurada; Hayato Matsuyama; Yasuyuki Tanahashi; Takio Kitazawa; Masahisa Yamada; Yurgen Wess, JOURNAL OF PHARMACOLOGICAL SCIENCES, 121, 2013 Muscarinic regulation of ATP sensitive K+ channels in mouse ileal smooth muscles, Yasuyuki Tanahashi; Toshihiro Unno; Hayato Matsuyama; Takio Kitazawa; Masahisa Yamada; Jurgen Wess; Komori Seiichi, JOURNAL OF PHARMACOLOGICAL SCIENCES, 121, 2013 Basic biochemical study for growth hormone secretagogue-receptor (GHS-R) 1a and plasma ghrelin in dogs, KITAZAWA Takio; ISHIDA Shoji; IWA Masaru; YAMAGATA Tomoya; OCHI Kenta; UCHIDA Eiji; HIRAGA Takeo; KAIYA Hiroyuki, Journal of the College of Dairying. Natural science, 36, (2) 295 - 310, 2012 04 A ganglion stimulant, 1, 1dimethyl-4phenylpiperazinium iodide(DMPP) stimulates both cholinergic and adrenergic neurons in isolated atrium of the mouse, Kenta Ochi; Hiroki Teraoka; Toshihiro Unno; Sei-ichi Komori; Masahisa Yamada; Takio Kitazawa, JOURNAL OF PHARMACOLOGICAL SCIENCES, 118, 2012 Neural M1 muscarinic receptor mediates inhibition of mouse colonic motility through activation of nitrergic nerves, Takaji Kondo; Hiroki Teraoka; Toshihiro Unno; Sei-ichi Komori; Masahisa Yamada; Takio Kitazawa, JOURNAL OF PHARMACOLOGICAL SCIENCES, 118, 2012 北海道におけるタンチョウの水銀汚染の原因についての検討, 岡本絵梨佳; 久道萌; 水野直治; 古賀公也; 志村良治; 中山翔; 池中良徳; 石塚真由美; 北澤多喜雄; 井上雅子; 百瀬邦和; 正富宏之; 寺岡宏樹; 寺岡宏樹, 日本鳥学会大会講演要旨集, 2011, 2011 09 13 北海道におけるタンチョウの水銀汚染の原因についての検討, 岡本絵梨佳; 久道萌; 水野直治; 古賀公也; 志村良治; 中山翔; 池中良徳; 石塚真由美; 北澤多喜雄; 井上雅子; 百瀬邦和; 正富宏之; 寺岡宏樹, 日本鳥学会大会講演要旨集, 2011, 2011 09 13 Role of M3 muscarinic receptor-coupled signalling molecule in the activation of muscarinic cation channels in mouse ileal smooth muscle cells, Toshihiro Unno; Michiko Inasaki; Hayato Matsuyama; Yasuyuki Tanahashi; Takio Kitazawa; Masahisa Yamada; Yurgen Wess; Seiichi Komori, JOURNAL OF PHARMACOLOGICAL SCIENCES, 115, 2011 脊椎動物共通プライマーを用いた大規模ディファレンシャル・ディスプレイ法によるダイオキシン応答遺伝子の探索, 鵜飼 典佳; 遠藤 大二; 池田 晴喜; 伊藤 知子; 伊東 志野; 金 恩英; 岩田 久人; 北澤 多喜雄; 平賀 武夫; 寺岡 宏樹, 日本獣医学会学術集会講演要旨集, 150回, 2010 09 エコトキシコゲノミクスへの応用を目的とした半網羅的ディファレンシャル・ディスプレイ法, 伊東 志野; 遠藤 大二; 池田 晴喜; 伊藤 知子; 鵜飼 典佳; 金 恩英; 岩田 久人; 北澤 多喜雄; 平賀 武夫; 寺岡 宏樹, The Journal of Toxicological Sciences, 35, (Suppl.) S181 - S181, 2010 05 Region-related contractile response to tetrodotoxin in mouse gastrointestinal tract, Yuichi Okuno; Takio Kitazawa, JOURNAL OF PHARMACOLOGICAL SCIENCES, 112, 2010 Ghrelin Stimulates Gastric Motility Through Activation of the Vago-Vagal Reflex Pathway in the Anesthetized Guinea-Pig, Takio Kitazawa; Takenori Onaga; Tatsuro Nakamura, GASTROENTEROLOGY, 136, (5) A534 - A534, 2009 05 Opposite inotropic action induced by activation of M2 and M3 muscarinic receptors in mouse atrium, Kitazawa Takio; Asakawa Koichi; Teraoka Hiroki; Unno Toshihiro; Komori Sei-ichi; Yamada Masahisa; Wess Jurgen, Journal of pharmacological sciences, 109, (1) 86 - 86, 2009 03 Ghrelin stimulates gastric motility of the anesthetized guinea-pig, Nakamura Tatsuro; Onaga Takenori; Kitazawa Takio, Journal of pharmacological sciences, 109, (1) 181 - 181, 2009 03 Ghrelin stimulates gastric motility of the anesthetized guinea-pig, Tatsuro Nakamura; Takenori Onaga; Takio Kitazawa, JOURNAL OF PHARMACOLOGICAL SCIENCES, 109, 2009 Opposite inotropic action induced by activation of M2 and M3 muscarinic receptors in mouse atrium, Takio Kitazawa; Koichi Asakawa; Hiroki Teraoka; Toshihiro Unno; Sei-ichi Komori; Masahisa Yamada; Jurgen Wess, JOURNAL OF PHARMACOLOGICAL SCIENCES, 109, 2009 Activation of cationic channels by diacylglycerol in mouse ileal smooth muscle cells, Sakamoto Takashi; Unno Toshihiro; Matsuyama Hayato; Kitazawa Takio; Taneike Tetsuro; Yamada Masahisa; Wess Jurgen; Komori Seiichi, Journal of pharmacological sciences, 106, (1) 270 - 270, 2008 02 M3 muscarinic receptor mediates carbachol-induced contraction of mouse uterine smooth muscle, Kitazawa Takio; Hirama Ryuichi; Masunaga Kozue; Nakamura Tatsuro; Asakawa Koichi; Cao Jinshan; Teraoka Hiroki; Unno Toshihiro; Komori Sei-ichi; Yamada Masahisa; Wess Jurgen; Taneike Tetsuro, Journal of pharmacological sciences, 106, (1) 108 - 108, 2008 02 Activation of cationic channels by diacylglycerol in mouse ileal smooth muscle cells, Takashi Sakamoto; Toshihiro Unno; Hayato Matsuyama; Takio Kitazawa; Tetsuro Taneike; Masahisa Yamada; Jurgen Wess; Seiichi Komori, JOURNAL OF PHARMACOLOGICAL SCIENCES, 106, 2008 M3 muscarinic receptor mediates carbachol-induced contraction of mouse uterine smooth muscle, Takio Kitazawa; Ryuichi Hirama; Kozue Masunaga; Tatsuro Nakamura; Koichi Asakawa; Jinshan Cao; Hiroki Teraoka; Toshihiro Unno; Sei-ichi Komori; Masahisa Yamada; Jurgen Wess; Tetsurci Taneike, JOURNAL OF PHARMACOLOGICAL SCIENCES, 106, 2008 Three distinct muscarinic signaling pathways for cationic channel activation in gut smooth muscle cells, Seiichi Komori; Takashi Sakamoto; Toshihiro Unno; Takio Kitazawa; Tetsuro Taneike; Masahisa Yamada; Jurgen Wess, NEUROGASTROENTEROLOGY AND MOTILITY, 19, 2007 10 Ghrelin-induced contractions and growth hormone secretagogue receptor expression in the chicken gastrointestinal tract, Takio Kitazawa; Hiroyuki Kaiya; Tetsuro Taneike, GASTROENTEROLOGY, 132, (4) A542 - A542, 2007 04 Synergistic activation of receptoroperated cationic channels by M-2 and M-3 muscarinic receptors in mouse ileal smooth muscle cells., Takashi Sakamoto; Toshihiro Unno; Hayato Matsuyama; Takio Kitazawa; Tetsuro Taneike; Masahisa Yamada; Wess Jurgen; Seiichi Komori, JOURNAL OF PHARMACOLOGICAL SCIENCES, 103, 2007 Different functional roles of M2 and M3 receptors in gastric contraction induced by carbachol and nerve stimulation, Takio Kitazawa; Kano Hashiba; Jinshan Cao; Toshihiro Unno; Sei-Ichi Komori; Masahisa Yamada; Jurgen Wess; Tetsuro Taneike, JOURNAL OF PHARMACOLOGICAL SCIENCES, 103, 2007 Characterization of prostanoid receptors present on adrenergic neurons of porcine uterine longitudinal muscle, Jinshan Cao; Takio Kitazawa; Tetsuro Taneike, JOURNAL OF PHARMACOLOGICAL SCIENCES, 103, 2007 Gastrointestinal-motility stimulating action of ghrelin in isolated chicken gastrointestinal tract, Takio Kitazawa; Tetsuro Taneike, ACTA PHARMACOLOGICA SINICA, 27, 2006 07 Endogenous prostaglandins (PGs) regulate spontaneous contractility of non-pregnant porcine myometrium, Jinshan Cao; Takio Kitazawa; Tetsuro Taneike, ACTA PHARMACOLOGICA SINICA, 27, 2006 07 Effects of ghrelin peptides on contractility of isolated chicken gastrointestinal tracts, T Kitazawa; T Taneike, JOURNAL OF PHARMACOLOGICAL SCIENCES, 100, 2006 モチリン・アンタゴニストの同時投与がGHSアゴニストの摂食抑制作用に及ぼす影響, カーン シャキル.イスラム; 百々功一; 上田博史; 種池哲朗; 北沢多喜雄; 保坂善真; 高梨契典; 豊後貴嗣, 日本家禽学会誌, 42, 2005 10 15 PHARMACOLOGICAL STUDY FOR CHARACTERIZATION OF THE NON-ADRENERGIC, NON-CHOLINERGIC (NANC) NEUROTRANSMITTER IN THE BOVINE UTERUS, KITAZAWA Takio; HATAKEYAMA Hirofumi; ONODERA Shuichi; SHIOMI Norio; TANEIKE Tetsuro, 日本平滑筋学会機関誌, 9, (2) "J - 47"-"J-64", 2005 08 01 , Pharmacological characteristics of electrical field stimulation (EFS)-induced mechanical responses in the bovine uterus indicate smooth muscle layer-specific variations in autonomic innervation (exclusive adrenergic innervation in the longitudinal muscle and non-adrenergic, non-cholinergic (NANC) innervation in the circular muscle). The aim of the present study was to characterize the neurotransmitter of NANC nerves in the bovine uterus using functional and biochemical approaches. EFS of uterine circular muscle caused atropine and guanethidine-resistant contraction, which was abolished by tetrodotoxin. The EFS-induced contraction was not decreased by pyrilamine, methysergide or indomethacin. In the presence of atropine and guanethidine, bioactive peptides (angiotensin II, bombesin, calcitonin-gene related peptide, enkephalins, galanin, neurokinin A, neurokinin B, substance P, neurotensin, vasoactive intestinal polypeptide), ATP and 5-hydroxytryptamine did not produce contraction of the myometrium. An acid extract of the bovine uterus (EBU) contracted both guinea-pig ileum longitudinal muscle (GPILM) and bovine uterus. Neither tetrodotoxin nor methysergide decreased the EBU-induced contraction. Atropine and pyrilamine inhibited the EBU-induced responses, but some of the contractions (5-15%) were resistant to both antagonists. On a Sephadex G50 column, smooth muscle contracting material was eluted in one peak. Active fractions were pooled and applied to a Sephadex G25 column to compare the elution volume of the active material with those of peptides of known molecular weights. The active material was eluted in one peak and the molecular weight was estimated to be 1200. The gel-filtrated fraction induced contraction of both GPILM and bovine uterus, and its contractile activity was abolished by α-chymotrypsin. In conclusion, uterotonic peptide was discovered in extract of the bovine uterus and was thought to be one of transmitter for NANC nerves. However, further studies are needed to clarify the structure of this unknown peptide and its physiological roles. GHS‐R AgonistおよびAntagonist投与がニワトリヒナの摂食量,消化管通過に及ぼす影響, 百々功一; 上田博史; 種池哲朗; 保坂善真; 北沢多喜雄; 豊後貴嗣, 日本家禽学会誌, 42, 2005 03 10 Involvement of the endogenous prostaglandins in regulation of spontaneous contraction in non-pregnant porcine uterus, JS Cao; T Kitazawa; T Taneike, JOURNAL OF PHARMACOLOGICAL SCIENCES, 97, 2005 Distribution and functional roles of 5-HT2A and 5-HT7 receptors in porcine uterus, T Iwamoto; T Kitazawa; JS Cao; T Taneike, JOURNAL OF PHARMACOLOGICAL SCIENCES, 97, 2005 In vivo and in vitro effects of peptide and nonpeptide ghrelin receptor agonists on gastric emptying in mice, Depoortere, I; T Kitazawa; B De Smet; T Peeters, REGULATORY PEPTIDES, 122, (1) 15 - 15, 2004 09 Region-related distribution of prostanoid receptors in non-pregnant porcine uterus, JS Cao; T Kitazawa; T Taneike, JOURNAL OF PHARMACOLOGICAL SCIENCES, 94, 2004 Gastroprokinetic action of growth hormone secretagogue receptor (GHS-R) agonists in mice, T Kitazawa; T Taneike; B De Smet; Depoortere, I; T Peeters, JOURNAL OF PHARMACOLOGICAL SCIENCES, 94, 2004 Presence of thromboxane A(2) (TP) receptor in the non-pregnant porcine uterus., JS Cao; T Kitazawa; T Taneike, JOURNAL OF PHARMACOLOGICAL SCIENCES, 91, 2003 ブタ子宮に存在する収縮性及び弛緩性EP受容体subtypeの解析, 曹 金山; 田島 剛; 沙衣布扎提; 北澤 多喜雄; 種池 哲朗, 日本獣医学会学術集会講演要旨集, 133回, 2002 03 Potentiation of motilin-induced contraction by nitric oxide synthase inhibition in the isolated chicken gastrointestinal tract, T Kitazawa; C Onodera; T Taneike, NEUROGASTROENTEROLOGY AND MOTILITY, 14, (1) 3 - 13, 2002 02 , 10.1046/j.1365-2982.2002.00298.x Subtype of prostanoid EP receptors present in the non-pregnant porcine myometrium, T Kitazawa; M Shayibuzhati; T Tajima; JS Cao; T Taneike, JAPANESE JOURNAL OF PHARMACOLOGY, 88, 2002 ブタ子宮におけるprostanoid受容体発現の筋層差, 田島 剛; 北澤 多喜雄; ミクラム・サイブザティ; 種池 哲朗, 日本獣医学会学術集会講演要旨集, 132回, 2001 09 ブタ子宮筋に存在するプロスタノイド受容体サブタイプの薬理学的解析, 沙衣布扎堤; 米克熱木; 畠山 洋文; 田島 剛; 北澤 多喜雄; 種池 哲朗, Journal of Smooth Muscle Research Japanese Section, 5, (2) J69 - J80, 2001 08 , 10.1540/heikatsukinzashi1997.5.J69 ブタ子宮におけるプロスタノイド受容体分布の筋層差, 北澤 多喜雄; 沙衣布扎提; 木; 田島 剛; 種池 哲朗, Journal of Smooth Muscle Research Japanese Section, 5, (1) J47 - J47, 2001 04 ブタ子宮筋のacetylcholine誘発性収縮に関与するmuscarine受容体subtypeの解明, 種池 哲朗; 内山 史一; 広瀬 佳代; 北澤 多喜雄, Journal of smooth muscle research. Japanese section, 2, (1) J - 49, 1998 04 30 Histamine mediates the muscle layer-specific responses in the isolated swine myometrium, T Kitazawa; H Shishido; T Sato; T Taneike, JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS, 20, (3) 187 - 197, 1997 06 , 10.1111/j.1365-2885.1997.tb00094.x EXCITATORY ACTION OF [LEU(13)]MOTILIN ON THE GASTROINTESTINAL SMOOTH-MUSCLE ISOLATED FROM THE CHICKEN, T KITAZAWA; T TANEIKE; A OHGA, PEPTIDES, 16, (7) 1243 - 1252, 1995 , 10.1016/0196-9781(95)00095-2 THE LEU(13)-MOTILIN (KW-5139)-EVOKED RELEASE OF ACETYLCHOLINE FROM ENTERIC NEURONS IN THE RABBIT DUODENUM, T KITAZAWA; A ISHII; K TANIYAMA, BRITISH JOURNAL OF PHARMACOLOGY, 109, (1) 94 - 99, 1993 05 , 10.1111/j.1476-5381.1993.tb13536.x
Research Grants & Projects
文部科学省, 科学研究費 基盤研究C, グレリン―モチリン系による消化管運動調節機構に関する比較生物学的研究, 201404, 201703, 北澤多喜雄 Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Role of ATP sensitive K+ channels in muscarinic regulation of intestinal contractility and its molecular identity, 201304, 201603, TANAHASHI Yasuyuki; WESS Jurgen; YAMADA Masahisa; KITAZAWA Takio; UNNO Toshihiro; KOMORI Seiichi; MATSUYAMA Hayato; NAGANO Hiroshi; TAKEUCHI Minoru; MURAKAMI Yuri; WANG Ban; ONO Maiki, The aim of this study was to elucidate the physiological roles of ATP-sensitive K+ channels (KATP channels) in muscarinic regulation of intestinal contractility and its mechanism. In addition, the properties of this channel were characterised. The results suggested that (1) the properties of the KATP channel, expressed in the intestinal smooth muscles, are similar to those of the Kir6.1/ SUR2B KATP channel in the vascular smooth muscles, and (2) M3 muscarinic subtype can inhibit the activities of the KATP channel, thereby contributing to the membrane depolarisation in smooth muscles, leading to their contraction. Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Age-related changes in ghrelin function, ghrelin concentration and ghrelin related molecules expression in the growing chickens., 201104, 201403, KITAZAWA Takio; KAIYA Hiroyuki; SAKAI Takafumi, Ghrelin contracted a chicken gastrointestinal tract. The ghrelin-induced contraction and expression of growth hormone secretagogue receptor (GHS-R1a) mRNA in the proventriculus decreased depending on age. Ghrelin concentrations in the proventriculus and plasma increased after hatching and reached plateau at 30 days. Expression of ghrelin and ghrelin-O-acetyltransferase (GOAT) mRNAs also tended to decrease depending on age. Expression of ghrelin mRNA did not show any correlation with plasma ghrelin. Inverse relationships between ghrelin concentration and GHS-R1a and GOAT mRNAs expression are indicative for a ghrelin-induced downregulation of the ghrelin receptor and synthetic enzyme. The expression pattern of ghrelin, GOAT and GHS-R1a mRNAs in the brain distinctly change in region-dependent manner on growing stage. The present results indicated that physiological role of ghrelin system changes in the brain and gastrointestinal tract of growing chickens. Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Elucidation of mechanisms for the generation of gut peristaltic reflex using various types of knockout mice., 201004, 201303, KOMORI Seiichi; UNNO Toshihiro; KITAZAWA Takio; IINO Satoshi; MATUYAMA Hayato; TANAHASHI Yasuyuki; JURGEN Wess; YAMADA Masahisa; SAKAI Hiroki; SAITOH Shouchirou; ANDOH Kumiko; KIMURA Kaori; SUGIYAMA Kyoko; MATSUI Ryuusuke; KATSURADA Taisuke; NAITO Kiyiotada; SOBUE Yuki; NAGANO Hiroshi; WAKATA Tomohiro, The cholinergic neurons play an important rolein the generation and regulation of gut peristaltic reflex. Acetylcholine released from the cholinergic neurons act on muscarinic receptors in various neurons and smooth muscles to produce peristalsis. However, the precise roles of muscarinic receptor subtypes have not yet been elucidated. In the present study, we analyzed gut peristalsis in muscarinic receptor subtype knockout mice. The results suggest that M2 subtype of muscarinic receptor plays an essential role in the generation of peristalsis and that this subtype may be a useful target for the development of new drugs to treat for various intestinal disorders, such as irritable bowel syndrome. Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Identification of novel ghrelin receptor in fish and clarification of the relationship between the receptor and biological action., 2008, 2010, KAIYA Hiroyuki; MIYAZATO Mikiya; KANGAWA Kenji; MATSUDA Kouhei; KITAZAWA Takio, In this study, we cloned cDNAs for four functional ghrelin receptors (GHS-R1a-1, 1a-2, 2a-1 and 2a-2) that composed of 360, 360, 366 and 367 amino acids, respectively in goldfish brain. Each receptor mRNA showed tissue-specific expression, suggesting that each receptor mediates tissue-specific action of ghrelin in goldfish. One species of ghrelin receptor, GHS-R1a has been known to be present in vertebrates other than fish examined so far. We tried to isolate new ligands for the new functional GHS-Ra, which is the novel type of ghrelin receptor, from tissue extracts of goldfish brain and intestine, but unfortunately the new ligand was not able to find out during the course of this study. Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Ghrelin peptides induced contractions and growth hormone secretagogue receptor expression in the chicken gastrointestinal tract, 200404, 200703, KITAZAWA Takio; ONAGA Takenori; IWANO Hidetomc, Background and aim. Ghrelin is the endogenous ligand for the growth hormone secretagogue receptor (GHS-R), and stimulates food intake and gastrointestinal (GI) motility in mammals. In chickens, on the other hand, ghrelin inhibits food intake. This discrepancy prompted us to investigate whether ghrelin affects gastrointestinal motility in the chicken. Methods, The contractile effect of chicken ghrelin was measured isometrically in muscle strips from different regions of the GI tract suspended in an organ bath. The effect of chicken ghrelin was compared with that of rat or human ghrelin. In addition, mRNA expression of ghrelin and GHS-R was examined by quantitative RT-PCR.
Results. Among the ghrelin peptides tested (rat, human and chicken ghrelin), only chicken ghrelin caused contraction of muscle strips from the GI tract. No effect was seen in des-octanoylated chicken ghrelin, suggesting that octanoylation at Ser^3 residue was essential for its activity. The contractile response to chicken ghrelin in the crop was not affected by tetrodotoxin (TTX), but that in the proventriculus was decreased by TTX and atropine to the same extent. D-Lys^3-GHRP-6 (a GHS-R antagonist) caused a transient contraction and inhibited the chicken ghrelin-induced contraction without affecting the 50 mM high-K^+-induced contraction. The amplitude of the chicken ghrelin-induced contraction was different among GI regions: highest in the crop and colon (70% of high-K^+-induced contraction), moderate in the esophagus and proventriculus (30%), and weak in the small intestine (10-20%) and the ranking order was consistent with GHS-R mRNA expression in each region (colon > crop = esophagus > proventriculus > duodenum =jejunum > ileum). GHS-R mRNA expression in the muscle preparations of crop and proventriculus without mucosa was 2-4 times higher than that in whole segments of crop and proventriculus, indicating that the GHS-R is mainly expressed in smooth muscle layers including enteric nerves. In contrast to the GHS-R expression, ghrelin mRNA was mainly expressed in the mucosa of proventriculus and duodenum.
Conclusion. These results indicate that ghrelin stimulates GI contractility and preferentially affects the motor activity of esophagus, crop and colon through myogenic and/or neurogenic mechanisms. These actions are mediated through the GHS-R, which is highly sensitive to chicken ghrelin Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Pharmacological characterization of prostanoid receptors present in the porcine uterus, 200104, 200403, TANEIKE Tetsuro; KITAZAWA Takio; YOKOTA Hiroshi, In the present research project, prostanoid receptor subtype and distribution of cyclooxygenase (COX) in the porcine uterus were characterized using pharmacological and biochemical techniques, and physiological roles of prostanoid in regulation of myometrial contractility were discussed. Contractile (EP1, EP3, FP and TP) and relaxant prostanoid receptors (DP, IP and EP2) were demonstrated in the porcine uterus muscle and these receptor populations were consistent with human. Therefore the porcine uterus might be a useful animal model for assessing the mechanical actions of prostanoid receptor agonists in the human uterus. Prostanoid receptors in the porcine uterus was suggested to distribute heterogeneously depending on smooth muscle types (longitudinal and circular muscle) and uterine regions (cornu, corpus and cervix). In the longitudinal muscle, responsiveness to contractile prostanoid receptor agonists was high compared with the circular muscle and decreased gradually from cornu to cervix. In contrary, responsiveness to relaxant prostanoid receptor agonists was low in the cornu and increased toward cervix. On the other hand, although responses to relaxant prostanoid receptor agonists were high in the circular muscle, regional difference in responsiveness to both contractile and relaxant prostanoid receptor agonists was not marked in comparison with the case of longitudinal muscle. The heterogeneous distribution of contractile and relaxant prostanoid receptor contributes to produce a gradient of the intra-luminal pressure from the cornu (high) to cervix (low) that is convenient for transportation of luminal contents. COX-1 but not COX-2 was expressed in the non-pregnant porcine uterus and distributed heterogeneously depending on muscle layer and uterine regions as did contractile prostanoid receptors. Inhibitors of COX and thromboxane A2 synthase decreased the spontaneous contraction of longitudinal muscle but not circular muscle. Present results suggest that prostanoid modulates, spontaneous myometrial contractility in pig. In the uterus of pregnant pig, COX-2 was expressed intensely and the responsiveness to a FP receptor agonist increased, suggesting a pregnancy-associated change in the physiological role of COX-prostanoid pathway. Further examinations might be necessary to clarify how steroid hormones regulate prostanoid receptor and COX expression, and myometrial motility. Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Charactrristics of motilin-induced contraction and its developmental change in the chicken gastrointestinal tract, 199904, 200203, KITAZAWA Takio; TAKEHARA Kazushige; YOKOTA Hiroshi, Chicken motilin (ch-MT) caused contractions of longitudinal muscle strips of the chicken stomach (proventriculus, Prov) though both myogenic and neurogenic (mostly cholinergic) mechanisms. On the other hand, ch-MT contracted the small intestine through only a myogenic mechanism. The sensitivity of gastrointestinal tracts to ch-MT was region-dependent (jejunum > ileum > duodenum > proventricculus > colon). Ch-MT also stimulated the gastric motility in vivo. The present experiments were designed to investigate developmental changes in responsiveness to ch-MT and in characteristics of electrical field stimulation (EFS)-induced responses, and interaction between NO and ch-MT. Immunohistochemical study was also carried out to examine the presence of ch-MT containing cells. Development of ch-MT-induced contraction : Amplitude of the ch-MT-induced contraction not-log EC_<50> value decreased gradually depending on hatching days(1-50 days), because of the reduction of indirect contraction through cholinergic nerves. But response to a Ach and 5-HT in Prov, and ch-MT-induced contraction in the ileum were not changed at every hatching day. These result suggest the physiological roles of MT in the chicken small intestinal motility ch-MT and NO interaction : L-Nitroarginine methyleaster (L-NAME) potentiated and L-arginine inhibited ch-MT and EFS-induced contractions in Prov without affecting the response to Ach and 5-HT. However, in the ileum and atropine-treated Prov, neither L-NAME nor L-arginine modified the responses to ch-MT. NO synthase inhibition potentiated the contractile response of ch-MT and EFS in Prov through reduction of endogenous NO-mediated presynaptic inhibition on neural ACh release. However, NOS inhibition did nor modify the myogenic (direct) action of ch-MT in gastric and intestinal smooth muscles. Innervation of chicken Prov : Prov was innervated 5 types of autonomic nerves, such as cholinergic excitatory nerve (0.1 - 20 Hz), nonadrenergicnoncholinergic (NANC) excitatory nerves (0.5 - 1 Hz and over 10Hz), NO inhibitory nerve (2 - 7 Hz), NANC, nonNO inhibitory nerve (over 7 Hz). Cholinergic and NO innervation was already completed on 1 day after hatching but NANC, nonNO inhibitory nerve was slowly. Immunohisyochemistry : Specific ch-MT antibody was produced using rabbits. MT-immunoreactive cells were present in the pylorus, duodenum, jejunum, ileum but not in the gizzard, Prov, rectum and caecum. MT containing cell present regions had a high sensitivity to ch-MT compared with other regions. MT is a motor stimulating hormone in the chicken intestinal tracts. Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Functional study for physiological role of 5-hydroxytryptamine7 receptors in porcine myometrium, 199804, 200103, TANEIKE Tetsuro; KITAZAWA Takio; YOKOTA Hiroshi, 5-Hydroxytryptamine (5-HT) inhibits the spontaneous contraction in longitudinal (LM) and circular muscles (CM) of the porcine uterus but CM is more sensitive to 5-HT than LM.The present experiments were designed to clarify the 5-HT receptor subtype mediating the inhibition, subcellular mechanisms of the inhibition and mechanisms underlying the muscle layer-dependent inhibition by 5-HT
Receptor subtype : On the basis of ranking order of 5-HT receptor agonists (5-carboxamidotryptamine>5-HT>5-methoxytryptamine>8-hydroxy-2-(di-n-propylamino) tetralin) and 5-HT receptor antagonists (methiothepin>methysergide>metergoline>mianserin>clozapine>spiperone), it was demonstrated that the 5-HT_7 receptor mediated the inhibition of myometrial contractility by 5-HT.
Inhibitory mechanism : Analysis of the 5-HT-induced inhibition suggested a following cascade of events in the porcine myometrium : 5-HT_7 receptor activation→activation of Gs→activation of adenylate cyclase→increase in cAMP→decrease in [Ca^<2+>]i and Ca^<2+>-sensitivity of the contractile elements→inhibition of muscle contractility.
5-HT_7receptor distribution : LM and CM layers contained a single class of [^3H]5-carboxamidotryptamine binding sites with a similar Kd value. However, Bmax in the CM was 4-times higher than that in the LM.The molecular study (reverse transcription polymerase chain reaction) demonstrated that the expression of 5-HT_7 receptor mRNA in the CM was higher than that in the LM.5-HT increased the cAMP in both muscle layers, but the increment in the CM was higher than that in the LM.Therefore, it was suggested that the different inhibition of the contractility by 5-HT was caused by muscle layer-related accumulation of cAMP (CM>LM), due to muscle-layer dependent distribution (CM>LM) of 5-HT_7 receptors. This heterogeneous distribution of 5-HT_7 receptor is quite unique and different from that of other receptors (muscarinc M_3 receptor, histamine H_1 receptor , endothelin A receptor, β and α2-adrenaline receptors, LM>CM) present in the porcine uterus.
Muscle layer-dependent inhibition : 5-HT_7 and β_2-adrenaline receptor-mediatd pathways were the main inhibitory mechanisms in the porcine myometrium and shared in the inhibition of contractility in a smooth muscle layer-dependent manner (LM : β_2-adrenoceptors, CM : 5-HT_7 receptors). The responses of 5-HT and isoproterenol differ from region to region, probably because of differences in the dominant subtype of 5-HT receptors and density of β_2-adrenaline receptors. Bioactive peptides and nitric oxide do not play a significant physiological role (as inhibitory agents) in the regulation of porcine uterine contractility. 日本学術振興会, 科学研究費助成事業, ブタの子宮縦走筋ならびに輸走筋におけるの2受容体性収縮機構の解明, 1993, 1993, 種池 哲朗; 寺岡 宏樹; 北澤 多喜雄, 1.収縮実験:【.encircled1.】ノルエピネフリン(NE,10nM-10muM,propranolol処置)は、Krebs液(Ca^<2+>2.5mM;37°C)液下で発情前期のブタ子宮角縦走筋(LM)標本を濃度依存性に収縮させた。この収縮は、TIX耐性で、alpha遮断薬phentolamine,alpha2遮断薬yohimbine(Yoh.10nM-1muM,pA2=8.37±0.06)により競合的に抑制されたが、alpha1遮断薬prazosin(Pra.1muM)では抑制されなかった。alpha2作動薬clonidine>xylazaine>>alpha1作動薬phenylephrine≧methoxamineの順番でLMを収縮させた。TMSで得られるLMの収縮は、TTX,phenotolamine,Yoh.(10-100nM)で抑制されたが、Pra.(1muM)では抑制されなかった。輪走筋(CM)はalpha1-とalpha2-作動薬に感受性を示さなかった。【.encircled2.】Ebashi-Kumagai液(Ca^<2+>0.4mM・Mg^<2+>0.2mM;28°C)下の収縮力価は、NE>phenylephrine>>methoxamineで、clonidine,xylazaineは100muMでも効果がなかった。NE収縮は、Yoh.(pA2=8.38±0.13)により競合的に抑制されたが、Pra.では非競合的に抑制された。受容体結合実験:粗製膜標本と放射性alpha1受容体リガンド^3H-prazosin、alpha2受容体^3H-rauwolscineとの結合が飽和性で特異的であった。^3H-prazosinの解離定数(kd値、nmol/1,n=4)と最大結合数(Bmax値、fmol/mg protein)は、LMで0.35±0.037,35.86±10.13,CMでは0.28±0.008,9.68±5.37であった。^3H-rauwolscineのKdとBmaxは、LMで3.09±0.26,255.61±25.15,CMでは2.86±0.19,62.64±6.26であった。以上より、ブタ子宮筋に存在するシナプス後受容体は、主にalpha2タイプであり、CMよりもLMに多く外因性および内因性NEの収縮反応の発現に関与していることを示している。しかし、このalpha2受容体機構は、低Ca^<2+>、低Mg^<2+>、低温下では受容体あるいはそれ以降のレベルで作動しずらくなるものと考えられる。
2.TMSの刺激頻度依存性にNE放出量は増加した。この放出増加はCa^2-free液中またはTTX処置で抑制された。誘発性のNE放出は、Yoh.,idazoxane,rauwolscineによりそれぞれ著明に増大したが、Pra.,clonidine,xylazaine,epinephrineによっては影響を受けなかった。これらより、シナプス前alpha2受容体を介するシナプス前抑制機構が作動しているか否かについては更なる検討が必要である。
3.静止時の子宮縦走筋のcAMP含量は約4 pmol/mg proteinで、IBMX(1mM)により増加する傾向が見られた。Isoproterenolは濃度依存性(0.1-100nM)にcAMP合成を刺激したが、収縮の最大反応を起こす1muMclonidineはIBMXの存在に関わらずcAMP含量に全く影響を与えなかった。従って、alpha2受容体刺激によるブタ子宮縦走筋の収縮反応にアデニレートシクラーゼ系は関与していないものと考えられた。 日本学術振興会, 科学研究費助成事業, Substance Pアミノ酸構造の系統発生過程での変化に関する基礎的研究, 1989, 1989, 北澤多喜雄 日本学術振興会, 科学研究費助成事業, コイ腸管膨大部におけるsubstance pの神経伝達物質としての可能性について, 1987, 1987, 北澤多喜雄 日本学術振興会, 科学研究費助成事業, コイ腸管膨大部の非Choline作動性収縮に関与する生理活性peptideの検索, 1986, 1986, 北澤多喜雄 日本学術振興会, 科学研究費助成事業, 消化管平滑筋神経支配様式の系統発生過程での変化に関する基礎的研究, 1984, 1984, 北澤多喜雄 日本学術振興会, 科学研究費助成事業, アドレナリン作動性刺激が消化管平滑筋に及ぼす影響に関する系統発生学的研究, 1983, 1983, 北澤 多喜雄
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Researcher Profile and Settings
Name
| KITAZAWA Takio |
Affiliation (Master)
| School of Veterinary Medicine, Department of Veterinary Science |
Affiliation & Job
| Rakuno Gakuen University |
| School of Veterinary Medicine Department of Veterinary Science |
Education
| 1979 04 - 1981 03 , Hokakido University Graduate School |
| 1975 04 - 1979 03 , Hokkaido University, Veterinary Medicine |
Association Memberships
| The Japanese Society of Veterinary Nursing |
| JAPAN SOCIETY OF SMOOTH MUSCLE RESEARCH |
| THE JAPANESE PHARMACOLOGICAL SOCIETY |
| THE JAPANESE SOCIETY OF VETERINARY SCIENCE |
Committee Memberships
| 2020 08 - Today, Japan Pharmacological Society, Pharmacological Educator |
Research Activities
Research Areas
| Life sciences, Morphology, anatomy |
| Life sciences, Veterinary medicine, Comparative Animal Pharmacology |
Research Interests
| musucarinic receptor, |
| motilin, ghrelin, |
| Comparative Biology, |
| Gut hormone, |
| Autonomic nerve, |
| Gastrointestinal motility, |
| Pharmacology, |
Published Papers
| Surface electromyography of the gluteus medius, vastus lateralis, and gastrocnemius muscles during sit-to-stand and stand-to-sit transitions in normal beagles., Kazuyuki Yoshikawa; Takio Kitazawa; Tadashi Sano; Takumi Ino; Tomoya Miyasaka; Kazuya Edamura, Research in Veterinary Science, 193, (105783) 1 - 5, 2025 06 |
| Physiolgical roles of ghrelin in the regulation of gastrointestinal motility in vertebrates, Shuangyi Zhang; Hiroyuki Kaiya; Takio Kitazawa, General and Comparative Endocrinology, 365, (1468) 1 - 10, 2025 02 |
| What is the function of motilin in the rabbit gastrointestinal tract?, Takio Kitazawa; Shuangyi Zhang, The Japanese Journal of Veterinary Research, 72, (3-4) 71 - 81, 2024 12 |
| The Concise Guide to PHARMACOLOGY 2023/24: G protein-coupled receptors., Stephen P H Alexander; Arthur Christopoulos; Anthony P Davenport; Eamonn Kelly; Alistair A Mathie; John A Peters; Emma L Veale; Jane F Armstrong; Elena Faccenda; Simon D Harding; Jamie A Davies; Maria Pia Abbracchio; George Abraham; Alexander Agoulnik; Wayne Alexander; Khaled Al-Hosaini; Magnus Bäck; Jillian G Baker; Nicholas M Barnes; Ross Bathgate; Jean-Martin Beaulieu; Annette G Beck-Sickinger; Maik Behrens; Kenneth E Bernstein; Bernhard Bettler; Nigel J M Birdsall; Victoria Blaho; Francois Boulay; Corinne Bousquet; Hans Bräuner-Osborne; Geoffrey Burnstock; Girolamo Caló; Justo P Castaño; Kevin J Catt; Stefania Ceruti; Paul Chazot; Nan Chiang; Bice Chini; Jerold Chun; Antonia Cianciulli; Olivier Civelli; Lucie H Clapp; Réjean Couture; Helen M Cox; Zsolt Csaba; Claes Dahlgren; Gordon Dent; Steven D Douglas; Pascal Dournaud; Satoru Eguchi; Emanuel Escher; Edward J Filardo; Tung Fong; Marta Fumagalli; Raul R Gainetdinov; Michael L Garelja; Marc de Gasparo; Craig Gerard; Marvin Gershengorn; Fernand Gobeil; Theodore L Goodfriend; Cyril Goudet; Lukas Grätz; Karen J Gregory; Andrew L Gundlach; Jörg Hamann; Julien Hanson; Richard L Hauger; Debbie L Hay; Akos Heinemann; Deron Herr; Morley D Hollenberg; Nicholas D Holliday; Mastgugu Horiuchi; Daniel Hoyer; László Hunyady; Ahsan Husain; Adriaan P IJzerman; Tadashi Inagami; Kenneth A Jacobson; Robert T Jensen; Ralf Jockers; Deepa Jonnalagadda; Sadashiva Karnik; Klemens Kaupmann; Jacqueline Kemp; Charles Kennedy; Yasuyuki Kihara; Takio Kitazawa; Pawel Kozielewicz; Hans-Jürgen Kreienkamp; Jyrki P Kukkonen; Tobias Langenhan; Dan Larhammar; Katie Leach; Davide Lecca; John D Lee; Susan E Leeman; Jérôme Leprince; Xaria X Li; Stephen J Lolait; Amelie Lupp; Robyn Macrae; Janet Maguire; Davide Malfacini; Jean Mazella; Craig A McArdle; Shlomo Melmed; Martin C Michel; Laurence J Miller; Vincenzo Mitolo; Bernard Mouillac; Christa E Müller; Philip M Murphy; Jean-Louis Nahon; Tony Ngo; Xavier Norel; Duuamene Nyimanu; Anne-Marie O'Carroll; Stefan Offermanns; Maria Antonietta Panaro; Marc Parmentier; Roger G Pertwee; Jean-Philippe Pin; Eric R Prossnitz; Mark Quinn; Rithwik Ramachandran; Manisha Ray; Rainer K Reinscheid; Philippe Rondard; G Enrico Rovati; Chiara Ruzza; Gareth J Sanger; Torsten Schöneberg; Gunnar Schulte; Stefan Schulz; Deborah L Segaloff; Charles N Serhan; Khuraijam Dhanachandra Singh; Craig M Smith; Leigh A Stoddart; Yukihiko Sugimoto; Roger Summers; Valerie P Tan; David Thal; Walter Wally Thomas; Pieter B M W M Timmermans; Kalyan Tirupula; Lawrence Toll; Giovanni Tulipano; Hamiyet Unal; Thomas Unger; Celine Valant; Patrick Vanderheyden; David Vaudry; Hubert Vaudry; Jean-Pierre Vilardaga; Christopher S Walker; Ji Ming Wang; Donald T Ward; Hans-Jürgen Wester; Gary B Willars; Tom Lloyd Williams; Trent M Woodruff; Chengcan Yao; Richard D Ye, British journal of pharmacology, 180 Suppl 2, 2023 10 |
| Does ghrelin regulate intestinal motility in rabbits? An in vitro study using isolated duodenal strips., Shuangyi Zhang; Hiroyuki Kaiya; Takio Kitazawa, General and comparative endocrinology, 344, 114384 - 114384, 2023 09 16 |
| Kinematic characteristics of canine hindlimb movement during sit-to-stand and stand-to-sit motions., Kazuyuki Yoshikawa; Takio Kitazawa; Tadashi Sano; Takumi Ino; Tomoya Miyasaka, Research in veterinary science, 162, 104944 - 104944, 2023 06 30 |
| Motilin is a regulator of gastric contraction in Japanese fire belly newts (Cynops pyrrhogaster), in vitro studies using isolated gastrointestinal strips of newts, rabbits, and chickens., Shuangyi Zhang; Hiroyuki Kaiya; Takio Kitazawa, General and comparative endocrinology, 330, 114140 - 114140, 2023 01 01 |
| Metabarcoding of feces and intestinal contents to determine carnivorous diets in red-crowned cranes in eastern Hokkaido, Japan., Haruka Kataoka; Nanami Koita; Natsuko Ito Kondo; Hiroshi C Ito; Momoko Nakajima; Kunikazu Momose; Hiroko Iima; Tomoo Yoshino; Tomoko Amano; Takio Kitazawa; Daiji Endoh; Nobuyoshi Nakajima; Hiroki Teraoka, The Journal of veterinary medical science, 84, (3) 358 - 367, 2022 03 03 |
| Pheasant motilin, its distribution and gastrointestinal contractility-stimulating action in the pheasant, Shuangyi Zhang; Hiroyuki Kaiya; Hiroki Teraoka; Takio Kitazawa, General and Comparative Endocrinology, Elsevier BV, 314, 113897 - 113897, 2021 12 |
| THE CONCISE GUIDE TO PHARMACOLOGY 2021/22: G protein-coupled receptors., Stephen Ph Alexander; Arthur Christopoulos; Anthony P Davenport; Eamonn Kelly; Alistair Mathie; John A Peters; Emma L Veale; Jane F Armstrong; Elena Faccenda; Simon D Harding; Adam J Pawson; Christopher Southan; Jamie A Davies; Maria Pia Abbracchio; Wayne Alexander; Khaled Al-Hosaini; Magnus Bäck; Nicholas M Barnes; Ross Bathgate; Jean-Martin Beaulieu; Kenneth E Bernstein; Bernhard Bettler; Nigel J M Birdsall; Victoria Blaho; Francois Boulay; Corinne Bousquet; Hans Bräuner-Osborne; Geoffrey Burnstock; Girolamo Caló; Justo P Castaño; Kevin J Catt; Stefania Ceruti; Paul Chazot; Nan Chiang; Bice Chini; Jerold Chun; Antonia Cianciulli; Olivier Civelli; Lucie H Clapp; Réjean Couture; Zsolt Csaba; Claes Dahlgren; Gordon Dent; Khuraijam Dhanachandra Singh; Steven D Douglas; Pascal Dournaud; Satoru Eguchi; Emanuel Escher; Edward J Filardo; Tung Fong; Marta Fumagalli; Raul R Gainetdinov; Marc de Gasparo; Craig Gerard; Marvin Gershengorn; Fernand Gobeil; Theodore L Goodfriend; Cyril Goudet; Karen J Gregory; Andrew L Gundlach; Jörg Hamann; Julien Hanson; Richard L Hauger; Debbie L Hay; Akos Heinemann; Morley D Hollenberg; Nicholas D Holliday; Mastgugu Horiuchi; Daniel Hoyer; László Hunyady; Ahsan Husain; Adriaan P IJzerman; Tadashi Inagami; Kenneth A Jacobson; Robert T Jensen; Ralf Jockers; Deepa Jonnalagadda; Sadashiva Karnik; Klemens Kaupmann; Jacqueline Kemp; Charles Kennedy; Yasuyuki Kihara; Takio Kitazawa; Pawel Kozielewicz; Hans-Jürgen Kreienkamp; Jyrki P Kukkonen; Tobias Langenhan; Katie Leach; Davide Lecca; John D Lee; Susan E Leeman; Jérôme Leprince; Xaria X Li; Tom Lloyd Williams; Stephen J Lolait; Amelie Lupp; Robyn Macrae; Janet Maguire; Jean Mazella; Craig A McArdle; Shlomo Melmed; Martin C Michel; Laurence J Miller; Vincenzo Mitolo; Bernard Mouillac; Christa E Müller; Philip Murphy; Jean-Louis Nahon; Tony Ngo; Xavier Norel; Duuamene Nyimanu; Anne-Marie O'Carroll; Stefan Offermanns; Maria Antonietta Panaro; Marc Parmentier; Roger G Pertwee; Jean-Philippe Pin; Eric R Prossnitz; Mark Quinn; Rithwik Ramachandran; Manisha Ray; Rainer K Reinscheid; Philippe Rondard; G Enrico Rovati; Chiara Ruzza; Gareth J Sanger; Torsten Schöneberg; Gunnar Schulte; Stefan Schulz; Deborah L Segaloff; Charles N Serhan; Leigh A Stoddart; Yukihiko Sugimoto; Roger Summers; Valerie P Tan; David Thal; Walter Wally Thomas; Pieter B M W M Timmermans; Kalyan Tirupula; Giovanni Tulipano; Hamiyet Unal; Thomas Unger; Celine Valant; Patrick Vanderheyden; David Vaudry; Hubert Vaudry; Jean-Pierre Vilardaga; Christopher S Walker; Ji Ming Wang; Donald T Ward; Hans-Jürgen Wester; Gary B Willars; Trent M Woodruff; Chengcan Yao; Richard D Ye, British journal of pharmacology, 178 Suppl 1, 2021 10 |
| Motilin Comparative Study: Structure, Distribution, Receptors, and Gastrointestinal Motility., Kitazawa Takio; Kaiya Hiroyuki, Frontier in Endocrinology, 12, (700884) 1 - 24, 2021 08 |
| Functional assessment of the gluteus medius, cranial part of the biceps femoris, and vastus lateralis in Beagle dogs based on a novel gait phase classification., Kazuyuki Yoshikawa; Sae Tsubakishita; Tadashi Sano; Takumi Ino; Tomoya Miyasaka; Takio Kitazawa, The Journal of veterinary medical science, 83, (1) 116 - 124, 2021 01 23 |
| Oxidative stress inducers potentiate 2,3,7,8-tetrachlorodibenzo-p-dioxin-mediated pre-cardiac edema in larval zebrafish, Katsuki TANAKA; Hikaru ADACHI; Hironobu AKASAKA; Junya TAMAOKI; Yuji FUSE; Makoto KOBAYASHI; Takio KITAZAWA; Hiroki TERAOKA, Journal of Veterinary Medical Science, Japanese Society of Veterinary Science, 83, (7) 1050 - 1058, 2021 |
| Functions of Muscarinic Receptor Subtypes in Gastrointestinal Smooth Muscle: A Review of Studies with Rewceptor-Knockout Mice, Yasuyuki Tanahashi; Seiichi Komori; Hayato Matsuyama; Takio Kitazawa; Toshihiro Unno, Int. J. Mol. Sci. 2021, 22, 926., 22, (926) 1 - 24, 2021 01 |
| Motilin- and ghrelin-induced contractions in isolated gastrointestinal strips from three species of frogs., Shuangyi Zhang; Hiroki Teraoka; Hiroyuki Kaiya; Takio Kitazawa, General and comparative endocrinology, 300, 113649 - 113649, 2021 01 01 |
| Cytochrome P450 Expression and Chemical Metabolic Activity before Full Liver Development in Zebrafish., Tasuku Nawaji; Natsumi Yamashita; Haruka Umeda; Shuangyi Zhang; Naohiro Mizoguchi; Masanori Seki; Takio Kitazawa; Hiroki Teraoka, Pharmaceuticals (Basel, Switzerland), 13, (12) , 2020 12 11 |
| Blood vessels are primary targets for 2,3,7,8-tetrachlorodibenzo-p-dioxin in pre-cardiac edema formation in larval zebrafish., Nijoukubo D, Adachi H, Kitazawa T, Teraoka H., Chemosphere., Elsevier BV, 254, (126808) 1 - 10, 2020 04 |
| Identification of pheasant ghrelin and motilin and their actions on contractility of the isolated gastrointestinal tract.Identification of pheasant ghrelin and motilin and their actions on contractility of the isolated gastrointestinal tract., Zhang S; Okuhara Y; Iijima M; Takemi S; Sakata I; Kaiya H; Teraoka H; Kitazawa T, Gen Comp Endocrinol., 285, (1) 12 - 19, 2020 01 |
| Genetic diversity of cytochrome P450 2A with different metabolic activities in domestic cats., Souta Sugiyama; Yasuhiro Uno; Tomoko Amano; Takio Kitazawa; Hiroki Teraoka, The Journal of veterinary medical science, 81, (7) 983 - 985, 2019 07 11 |
| Genetic diversity of cytochrome P450 1A2 with different metabolic activities in domestic cats., Sugiyama S; Uno Y; Amano T; Kitazawa T; Teraoka H, J. Vte.Med. Sci, Japanese Society of Veterinary Science, 81, (7) 980 - 982, 2019 07 |
| Limited expression of functional cytochrome p450 2c subtypes in the liver and small intestine of domestic cats., Ono Y; Sugiyama S; Matsushita M; Kitazawa T; Amano T; Uno Y; Ikushiro S; Teraoka H, Xenobiotica., Informa UK Limited, doi: 10.1080/00498254.2018.148, (6) 1 - 9, 2019 06 |
| Transcription profiling of cytocrome P450 genes in the liver of adult zebrafish, Danio rerio., Kubota A; Kawai Y; Yamashita N; Lee JS; Kondoh D; Zhang S; Nishi Y; Suzuki K; Kitazawa T; Teraoka H, The Journal of Toxicological Sciences, (一社)日本毒性学会, 44, (Suppl.) S322 - S322, 2019 06 |
| Regulation of Gastrointestinal Motility by Motilin and Ghrelin in Vertebrates, Kitazawa Takio; Kaiya Hiroyuki, Frontier in Endocrinology, doi: 10.3389/fendo.2019.00278, (MAY) 1 - 19, 2019 05 |
| Genetic diversity of cytochrome P450 3A with different metabolic activity in domestic cats., Sugiyama S; Uno Y; Amano T; Kitazawa T; Teraoka H, J. Veterinary Medical Science, Japanese Society of Veterinary Science, 81, (4) 598 - 600, 2019 04 |
| A verification study of gastrointestinal motility-stimulating action of guinea-pig motilin using isolated gastrointestinal strips from rabbits and guinea-pigs, Takio Kitazawa; Rio Harada; Ichiro Sakata; Takafumi Sakai; Hiroyuki Kaiya, General and Compaartive Endocrinology, 100, (1) 19 - 28, 2019 04 |
| Alpha1B-adrenoceptor-mediated positive inotropic and positive chronotropic actions in the mouse atrium, Shuangyi Zhang Reia Takahashi; Natsumi Yamashita; Hiroki Teraoka; Takio Kitazawa, European J Pharmacology, 839, (1) 82 - 88, 2018 11 |
| Contamination Status of Seven Elements in Hooded Cranes Wintering in South-West Kyushu, Japan: Comparison with Red-Crowned Cranes in Hokkaido, Japan, Hiroki Teraoka; Hasumi Miyagi; Yuko Haraguchi; Kozo Takase; Takio Kitazawa; Jun Noda, Archives of Environmental Contamination and Toxicology, Springer New York LLC, doi: 10.1007/s00244-018-0541-y, 1 - 9, 2018 05 31 |
| Post-operative rehabilitation of a dog with tetraparesis resulting from cervical intervertebral disc herniation., Kitazawa Takio, Veterinary Nursing, 日本動物看護学会, 22, (2) 35 - 40, 2018 03 |
| Aroclor 1254 and BDE-47 inhibit dopaminergic function manifesting as changes in locomotion behaviors in zebrafish embryos, Yasuaki Tanaka; Mari Fujiwara; Asako Shindo; Guojun Yin; Takio Kitazawa; Hiroki Teraoka, Chemosphere, Elsevier BV, 193, 1207 - 1215, 2018 02 |
| Structural determination, distribution, and physiological actions of ghrelin in the guinea pig, Yuji Okuhara; Hiroyuki Kaiya; Hiroki Teraoka; Takio Kitazawa, Peptides, Elsevier Inc., 99, 70 - 81, 2018 01 01 |
| Dexamethasone-induced hepatomegaly and steatosis in larval zebrafish, Guojun Yin; Liping Cao; Jinliang Du; Rui Jia; Takio Kitazawa; Akira Kubota; Hiroki Teraoka, JOURNAL OF TOXICOLOGICAL SCIENCES, 42, (4) 455 - 459, 2017 08 |
| Does motilin peptide regulate gastrointestinal motility of zebrafish? An in vitro study using isolated intestinal strips, Takio Kitazawa; Maria Yoshida; Hiroki Teraoka; Hiroyuki Kaiya, GENERAL AND COMPARATIVE ENDOCRINOLOGY, 249, 15 - 23, 2017 08 |
| The chicken is an interesting animal for study of the functional role of ghrelin in the gastrointestinal tract, Takio Kitazawa; Hiroki Teraoka; Hiroyuki Kaiya, ENDOCRINE JOURNAL, 64, S5 - S9, 2017 |
| Characterization of feline cytochrome P450 2B6, Gaku Okamatsu; Tetsuya Komatsu; Yuka Ono; Hiroki Inoue; Tsuyoshi Uchide; Takenori Onaga; Daiji Endoh; Takio Kitazawa; Takeo Hiraga; Yasuhiro Uno; Hiroki Teraoka, XENOBIOTICA, 47, (2) 93 - 102, 2017 |
| Functional expression and comparative characterization of four feline P450 cytochromes using fluorescent substrates, Gaku Okamatsu; Kei Kawakami; Tetsuya Komatsu; Takio Kitazawa; Yasuhiro Uno; Hiroki Teraoka, XENOBIOTICA, 47, (11) 951 - 961, 2017 |
| Pharmacological characterization of muscarinic receptor subtypes involved in carbachol-induced contraction of the chicken proventriculus, Kitazawa Takio; Nahoko Kondo; Tetsuro Taneike, SM. J. Gastroenterol., 2, (1) 1003 - 1012, 2016 10 |
| ムスカリン受容体を介した胃粘膜細胞の分化・増殖制御, 松山 勇人; 内藤 清惟; 和田 善明; 永野 宏; 齋藤 正一郎; 酒井 洋樹; 棚橋 靖行; 北澤 多喜雄; 小森 成一; 海野 年弘, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 159回, 497 - 497, 2016 08 |
| Protective effect of prostacyclin against pre-cardiac edema caused by 2,3,7,8-tetrachlorodibenzo-p-dioxin and a thromboxane receptor agonist in developing zebrafish, Daisuke Nijoukubo; Yasuaki Tanaka; Yuki Okuno; Guojun Yin; Takio Kitazawa; Richard E. Peterson; Akira Kubota; Hiroki Teraoka, CHEMOSPHERE, 156, 111 - 117, 2016 08 |
| Molecular cloning of motilin and mechanism of motilin-induced gastrointestinal motility in Japanese quail, Auvijit Saha Apu; Anupom Mondal; Takio Kitazawa; Shota Takemi; Takafumi Sakai; Ichiro Sakata, GENERAL AND COMPARATIVE ENDOCRINOLOGY, 233, 53 - 62, 2016 07 |
| Effects of ghrelin and motilin on smooth muscle contractility of the isolated gastrointestinal tract from the bullfrog and Japanese fire belly newt, Takio Kitazawa; Misato Shimazaki; Ayumi Kikuta; Noriko Yaosaka; Hiroki Teraoka; Hiroyuki Kaiya, GENERAL AND COMPARATIVE ENDOCRINOLOGY, 232, 51 - 59, 2016 06 |
| Regulation of heart contractility by M |
| Dexamethasone-induced hepatomegaly and fatty liver in zebrafish, YIN Guojun; NIJOUKUBO Daisuke; KITAZAWA Takio; CAO Liping; DUB Jinliang; TERAOKA Hiroki, Annual Meeting of the Japanese Society of Toxicology, The Japanese Society of Toxicology, 43, P - 35, 2016 |
| Accumulation properties of inorganic mercury and organic mercury in the red-crowned crane Grus japonensis in east Hokkaido, Japan, Hiroki Teraoka; Erika Okamoto; Moe Kudo; Shouta M. M. Nakayama; Yoshinori Ikenaka; Mayumi Ishizuka; Tetsuya Endo; Takio Kitazawa; Takeo Hiraga, ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 122, 557 - 564, 2015 12 |
| Identification and functional characterization of novel feline cytochrome P450 2A, Gaku Okamatsu; Tetsuya Komatsu; Akira Kubota; Takenori Onaga; Tsuyoshi Uchide; Daiji Endo; Rikio Kirisawa; Guojun Yin; Hiroki Inoue; Takio Kitazawa; Yasuhiro Uno; Hiroki Teraoka, XENOBIOTICA, 45, (6) 503 - 510, 2015 06 |
| Correlation of ghrelin concentration and ghrelin, ghrelin-O-acetyltransferase (GOAT) and growth hormone secretagogue receptor 1a mRNAs expression in the proventriculus and brain of the growing chicken, Takio Kitazawa; Takeo Hiraga; Hiroki Teraoka; Noriko Yaosaka; Hiroyuki Kaiya, PEPTIDES, 63, 134 - 142, 2015 01 |
| What is the function of motilin in the rabbit gastrointestinal tract?, Takio Kitazawa; Shuangyi Zhang, The Japanese Journal of Veterinary Research, 72, (3-4) 71 - 81, 2014 12 |
| Involvement of COX2-thromboxane pathway in TCDD-induced precardiac edema in developing zebrafish, Hiroki Teraoka; Yuki Okuno; Daisuke Nijoukubo; Ayumi Yamakoshi; Richard E. Peterson; John J. Stegeman; Takio Kitazawa; Takeo Hiraga; Akira Kubota, AQUATIC TOXICOLOGY, 154, 19 - 26, 2014 09 |
| Roles of M-2 and M-3 muscarinic receptors in the generation of rhythmic motor activity in mouse small intestine, Y. Tanahashi; N. Waki; T. Unno; H. Matsuyama; S. Iino; T. Kitazawa; M. Yamada; S. Komori, NEUROGASTROENTEROLOGY AND MOTILITY, 25, (10) E687 - E697, 2013 10 |
| Age-dependent reduction of ghrelin- and motilin-induced contractile activity in the chicken gastrointestinal tract, Takio Kitazawa; Akiko Yoshida; Takuya Tamano; Hiroki Teraoka; Hiroyuki Kaiya, Peptides, 43, 88 - 95, 2013 05 |
| A ganglionic stimulant, 1,1-dimethyl-4-phenylpiperazinium, caused both cholinergic and adrenergic responses in the isolated mouse atrium, Kenta Ochi; Hiroki Teraoka; Toshihiro Unno; Sei-Ichi Komori; Masahisa Yamada; Takio Kitazawa, European Journal of Pharmacology, 704, (1-3) 7 - 14, 2013 03 15 |
| Large-Scale Survey of Mitochondrial D-Loop of the Red-Crowned Crane Grus japonensis in Hokkaido, Japan by Convenient Genotyping Method, Yoshiaki Miura; Akira Shiomi; Junya Shiraishi; Kohei Makita; Mitsuhiko Asakawa; Takio Kitazawa; Takeo Hiraga; Yulia Momose; Kunikazu Momose; Hiroyuki Masatomi; Hiroki Teraoka, JOURNAL OF VETERINARY MEDICAL SCIENCE, 75, (1) 43 - 47, 2013 01 |
| Evidence for M2 and M3 Muscarinic receptor involvement in cholinergic excitatory junction potentials through synergistic activation of Cation channels in the longitudinal muscle of mouse ileum, Hayato Matsuyama; Yasuyuki Tanahashi; Takio Kitazawa; Masahisa Yamada; Seiichi Komori; Toshihiro Unno, Journal of Pharmacological Sciences, 121, (3) 227 - 236, 2013 |
| Origin of three red-crowned cranes Grus japonensis found in northeast Honshu and West Hokkaido, Japan, from 2008 to 2012, Yoshiaki Miura; Junya Shiraishi; Akira Shiomi; Takio Kitazawa; Takeo Hiraga; Fumio Matsumoto; Hiroki Teraoka; Hiroyuki Masatomi, Journal of Veterinary Medical Science, 75, (9) 1241 - 1244, 2013 |
| Immunohistochemical and functional studies for M 3 muscarinic receptors and cyclo-oxygenase-2 expressed in the mouse atrium, N. Harada; K. Ochi; N. Yaosaka; H. Teraoka; T. Hiraga; T. Iwanaga; T. Unno; S. Komori; M. Yamada; T. Kitazawa, Autonomic and Autacoid Pharmacology, 32, (3) 41 - 52, 2012 10 |
| Ghrelin does not affect gastrointestinal contractility in rainbow trout and goldfish in vitro, Takio Kitazawa; Kentaro Itoh; Noriko Yaosaka; Keisuke Maruyama; Kouhei Matsuda; Hiroki Teraoka; Hiroyuki Kaiya, GENERAL AND COMPARATIVE ENDOCRINOLOGY, 178, (3) 539 - 545, 2012 09 |
| Changes of Mercury Contamination in Red-Crowned Cranes, Grus japonensis, in East Hokkaido, Japan, Hiroki Teraoka; Yukari Tagami; Moe Kudo; Yoshiaki Miura; Erika Okamoto; Fumio Matsumoto; Kimiya Koga; Akiko Uebayashi; Ryoji Shimura; Masako Inoue; Kunikazu Momose; Hiroyuki Masatomi; Takio Kitazawa; Takeo Hiraga; Annamalai Subramanian, ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 63, (1) 153 - 160, 2012 07 |
| Differential Display System with Vertebrate-Common Degenerate Oligonucleotide Primers: Uncovering Genes Responsive to Dioxin in Avian Embryonic Liver, Hiroki Teraoka; Shino Ito; Haruki Ikeda; Akira Kubota; M. M. Abou Elmagd; Takio Kitazawa; Eun-Young Kim; Hisato Iwata; Daiji Endoh, ENVIRONMENTAL SCIENCE & TECHNOLOGY, 46, (1) 27 - 33, 2012 01 |
| Expression of Two Novel Cytochrome P450 3A131 and 3A132 in Liver and Small Intestine of Domestic Cats, Kouichi Honda; Tetsuya Komatsu; Fumika Koyama; Akira Kubota; Kei Kawakami; Hiroyuki Asakura; Yasuhiro Uno; Takio Kitazawa; Takeo Hiraga; Hiroki Teraoka, JOURNAL OF VETERINARY MEDICAL SCIENCE, 73, (11) 1489 - 1492, 2011 11 |
| Muscarinic receptor subtypes involved in regulation of colonic motility in mice: Functional studies using muscarinic receptor-deficient mice, Takaji Kondo; Miwa Nakajima; Hiroki Teraoka; Toshihiro Unno; Sei-ichi Komori; Masahisa Yamada; Takio Kitazawa, EUROPEAN JOURNAL OF PHARMACOLOGY, 670, (1) 236 - 243, 2011 11 |
| Molecular identification of ghrelin receptor (GHS-R1a) and its functional role in the gastrointestinal tract of the guinea-pig, Takio Kitazawa; Tatsuro Nakamura; Atsuki Saeki; Hiroki Teraoka; Takeo Hiraga; Hiroyuki Kaiya, PEPTIDES, 32, (9) 1876 - 1886, 2011 09 |
| Colon-specific contractile responses to tetrodotoxin in the isolated mouse gastrointestinal tract, Y. Okuno; T. Kondo; A. Saeki; E. Uchida; H. Teraoka; T. Kitazawa, Autonomic and Autacoid Pharmacology, 31, (1-2) 21 - 30, 2011 01 |
| Molecular Cloning and Expression of Cytochrome P450 2D6 in the Livers of Domestic Cats, Tetsuya Komatsu; Kouichi Honda; Akira Kubota; Takio Kitazawa; Takeo Hiraga; Hiroki Teraoka, JOURNAL OF VETERINARY MEDICAL SCIENCE, 72, (12) 1633 - 1636, 2010 12 |
| Ghrelin stimulates gastric motility of the guinea pig through activation of a capsaicin-sensitive neural pathway: in vivo and in vitro functional studies, T. Nakamura; T. Onaga; T. Kitazawa, NEUROGASTROENTEROLOGY AND MOTILITY, 22, (4) 446 - +, 2010 04 |
| Muscarinic Receptor Subtypes Mediating Ca2+ Sensitization of Intestinal Smooth Muscle Contraction: Studies with Receptor Knockout Mice, Mayuko Suguro; Hayato Matsuyama; Yasuyuki Tanahashi; Toshihiro Unno; Takio Kitazawa; Masahisa Yamada; Seiichi Komori, JOURNAL OF VETERINARY MEDICAL SCIENCE, 72, (4) 443 - 451, 2010 04 |
| Molecular cloning of growth hormone secretagogue-receptor and effect of quail ghrelin on gastrointestinal motility in Japanese quail, Takio Kitazawa; Yoshimi Maeda; Hiroyuki Kaiya, REGULATORY PEPTIDES, 158, (1-3) 132 - 142, 2009 11 |
| M-3 Muscarinic Receptors Mediate Positive Inotropic Responses in Mouse Atria: A Study with Muscarinic Receptor Knockout Mice, Takio Kitazawa; Koichi Asakawa; Tatsuro Nakamura; Hiroki Teraoka; Toshihiro Unno; Sei-ichi Komori; Masahisa Yamada; Juergen Wess, JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 330, (2) 487 - 493, 2009 08 |
| A non-selective cationic channel activated by diacylglycerol in mouse intestinal myocytes, Takashi Sakamoto; Hayato Matsuyama; Masayuki Yamamoto; Yasuyuki Tanahashi; Takio Kitazawa; Tetsuro Taneike; Seiichi Komori; Toshihiro Unno, EUROPEAN JOURNAL OF PHARMACOLOGY, 599, (1-3) 54 - 57, 2008 12 |
| Excitatory and inhibitory 5-hydroxytryptamine (5-HT) receptors expressed in the isolated porcine uterine muscles, Tatsuro Nakamura; Takio Kitazawa; Jinshan Cao; Takuya Iwamoto; Hiroki Teraoka; Koichi Kadota; Tetsuro Taneike, EUROPEAN JOURNAL OF PHARMACOLOGY, 600, (1-3) 123 - 129, 2008 12 |
| Muscarinic receptor subtypes involved in carbachol-induced contraction of mouse uterine smooth muscle, Takio Kitazawa; Ryuichi Hirama; Kozue Masunaga; Tatsuro Nakamura; Koichi Asakawa; Jinshan Cao; Hiroki Teraoka; Toshihiro Unno; Sei-ichi Komori; Masahisa Yamada; Juergen Wess; Tetsuro Taneike, NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 377, (4-6) 503 - 513, 2008 06 |
| Characterization of prostanoid receptors present on adrenergic neurons innervating the porcine uterine longitudinal muscle, Jinshan Cao; Tatsuro Nakamura; Takio Kitazawa; Naoko Yamashiki; Tsubasa Yamamoto; Tetsuro Taneike, PROSTAGLANDINS & OTHER LIPID MEDIATORS, 86, (1-4) 26 - 34, 2008 06 |
| 高齢犬におけるトラマドールの薬物動態, 内田 陽介; 北澤 多喜雄; 井上 博紀; 山下 和人, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 144回, 152 - 152, 2007 08 |
| Three distinct muscarinic signalling pathways for cationic channel activation in mouse gut smooth muscle cells, Takashi Sakamoto; Toshihiro Unno; Takio Kitazawa; Tetsuro Taneike; Masahisa Yamada; Jurgen Wess; Masakazu Nishimura; Seiichi Komori, JOURNAL OF PHYSIOLOGY-LONDON, 582, (1) 41 - 61, 2007 07 |
| Contractile effects of ghrelin-related peptides on the chicken gastrointestinal tract in vitro, Takio Kitazawa; Hiroyuki Kaiya; Tetsuro Taneike, PEPTIDES, 28, (3) 617 - 624, 2007 03 |
| Functional roles of muscarinic M-2 and M-3 receptors in mouse stomach motility: Studies with muscarinic receptor knockout mice, Takio Kitazawa; Kano Hashiba; Jinshan Cao; Toshihiro Unno; Sei-ichi Komori; Masahisa Yamada; Jurgen Wess; Tetsuro Taneike, EUROPEAN JOURNAL OF PHARMACOLOGY, 554, (2-3) 212 - 222, 2007 01 |
| Endogenous prostaglandins regulate spontaneous contractile activity of uterine strips isolated from non-pregnant pigs, Jinshan Cao; Takio Kitazawa; Kazushige Takehana; Tetsuro Taneike, PROSTAGLANDINS & OTHER LIPID MEDIATORS, 81, (3-4) 93 - 105, 2006 12 |
| Pharmacological characterization of 5-hydroxytryptamine-induced contraction in the chicken gastrointestinal tract, T. Kitazawa; H. Ukai; S. Komori; T. Taneike, Autonomic and Autacoid Pharmacology, 26, (2) 157 - 168, 2006 |
| Intracerebroventricular Administration of Growth Hormone Releasing Peptide-6 (GHRP-6) Inhibits Food Intake, but not Food Retention of Crop and Stomaeh in Neonatal Chicks, Md. Sakirul Islam Khan; Koh-Ichi Dodo; Kazuki Yahata; Sayuri Nishimoto; Hiroshi Ueda; Tetsuro Taneike; Takio Kitazawa; Yoshinao Hosaka; Takashi Bungo, Journal of Poultry Science, 43, (1) 35 - 40, 2006 |
| An in Vitro Study on Spontaneous Contractility and Drug Responsiveness of Porcine Myometrium During Three Ovarian Stages, KITAZAWA Takio; MIYAZAKI Reiko; ISOE Gentaro; CAO Jinshan; TANEIKE Tetsuro, The Japanese Journal of Swine Husbandry Research, The Japanese Society of Swine Science, 42, (4) 165 - 177, 2005 12 26 |
| Gastric motor effects of peptide and non-peptide ghrelin agonists in mice in vivo and in vitro, T Kitazawa; B De Smet; K Verbeke; Depoortere, I; TL Peeters, GUT, 54, (8) 1078 - 1084, 2005 08 |
| Uterine region-dependent differences in responsiveness to prostaglandins in the non-pregnant porcine myometrium, JS Cao; M Yosida; T Kitazawa; T Taneike, PROSTAGLANDINS & OTHER LIPID MEDIATORS, 75, (1-4) 105 - 122, 2005 01 |
| Intracerebroventricular administration of chicken motilin does not induce hyperphagia in meat-type chicks, T Bungo; H Ueda; T Kitazawa; T Taneike; M Furuse, PHYSIOLOGY & BEHAVIOR, 82, (2-3) 199 - 203, 2004 09 |
| Thromboxane A(2) (TP) receptor in the non-pregnant porcine myometrium and its role in regulation of spontaneous contractile activity, JS Cao; A Wakatsuki; M Yoshida; T Kitazawa; T Taneike, EUROPEAN JOURNAL OF PHARMACOLOGY, 485, (1-3) 317 - 327, 2004 02 |
| Pregnancy-associated changes in responsiveness of the porcine myometrium to bioactive substances, T Kitazawa; H Hatakeyama; JS Cao; T Taneike, EUROPEAN JOURNAL OF PHARMACOLOGY, 469, (1-3) 135 - 144, 2003 05 |
| 5-HT7 receptor-mediated relaxation of the oviduct in nonpregnant proestrus pigs, M Inoue; T Kitazawa; J Cao; T Taneike, EUROPEAN JOURNAL OF PHARMACOLOGY, 461, (2-3) 207 - 218, 2003 02 |
| In vitro pharmacological characterization of the prostanoid receptor population in the non-pregnant porcine myometrium, JS Cao; M Shayibuzhati; T Tajima; T Kitazawa; T Taneike, EUROPEAN JOURNAL OF PHARMACOLOGY, 442, (1-2) 115 - 123, 2002 05 |
| 5-HT7 receptor and beta(2)-adrenoceptor share in the inhibition of porcine uterine contractility in a muscle layer-dependent manner, T Kitazawa; K Nakagoshi; H Teraoka; T Taneike, EUROPEAN JOURNAL OF PHARMACOLOGY, 433, (2-3) 187 - 197, 2001 12 |
| Muscle layer- and region-dependent distributions of oxytocin receptors in the porcine myometrium, T Kitazawa; T Kajiwara; A Kiuchi; H Hatakeyama; T Taneike, PEPTIDES, 22, (6) 963 - 974, 2001 06 |
| Change of the involvement of 5-HT3 receptor in the gastric motor stimulating actions of KW-5139 (Leu(13)-motilin acetate) in the recovered and post-operative periods in dogs, T Yokoyama; K Takasaki; T Kitazawa; A Karasawa; H Kusaka, LIFE SCIENCES, 67, (19) 2281 - 2292, 2000 09 |
| Smooth muscle layer-dependent distribution of 5-hydroxytryptamine(7) receptor in the porcine myometrium, T Kitazawa; Y Yamada; H Iwano; H Yokota; A Yuasa; T Taneike, BRITISH JOURNAL OF PHARMACOLOGY, 130, (1) 79 - 89, 2000 05 |
| Characterization of functional endothelin receptors in the porcine myometrium, M Isaka; K Takaoka; Y Yamada; Y Abe; T Kitazawa; T Taneike, PEPTIDES, 21, (4) 543 - 551, 2000 04 |
| The mechanisms of alpha(2)-adrenoceptor agonist-induced contraction in longitudinal muscle of the porcine uterus, T Kitazawa; Y Maezono; T Taneike, EUROPEAN JOURNAL OF PHARMACOLOGY, 390, (1-2) 185 - 195, 2000 02 |
| 5-Hydroxytryptamineのブタ子宮筋収縮抑制作用とその細胞内メカニズム, 北澤 多喜雄; 山田 裕子; 宮庄 拓; 岩野 英知; 横田 博; 湯浅 亮; 種池 哲朗, 獣医生化学, 37, 39 - 50, 2000 |
| Mechanisms of 5-hydroxytryptamine-induced inhibition in the porcine myometrium, T Kitazawa; K Takaoka; T Taneike, JOURNAL OF AUTONOMIC PHARMACOLOGY, 19, (2) 65 - 75, 1999 04 |
| Characterization of the muscarinic receptor subtype that mediates the contractile response of acetylcholine in the swine myometrium, T Kitazawa; F Uchiyama; K Hirose; T Taneike, EUROPEAN JOURNAL OF PHARMACOLOGY, 367, (2-3) 325 - 334, 1999 02 |
| Smooth muscle layer-specific variations in the autonomic innervation of bovine myometrium, T Taneike; T Kitazawa; H Funakura; K Asanuma; S Tsuji; M Yamada; H Teraoka; A Ohga, GENERAL PHARMACOLOGY, 32, (1) 91 - 100, 1999 01 |
| Involvement of 5-hydroxytryptamine(7) receptors in inhibition of porcine myometrial contractility by 5-hydroxytryptamine, T Kitazawa; O Kubo; M Satoh; T Taneike, BRITISH JOURNAL OF PHARMACOLOGY, 123, (2) 173 - 182, 1998 01 |
| Functional characterization of neural and smooth muscle motilin receptors in the chicken proventriculus and ileum, T Kitazawa; T Taneike; A Ohga, REGULATORY PEPTIDES, 71, (2) 87 - 95, 1997 08 |
| In vitro and in vivo investigations of the effect of Leu13-porcine motilin on pig gastrointestinal motility, T Kitazawa; S Kikui; T Taneike; A Ohga; K Sugisaki; N Tomizawa; S Hara, DIGESTIVE PHYSIOLOGY IN PIGS, (88) 158 - 162, 1997 |
| Does motilin stimulate the gastrointestinal motility of the pig? In vitro study using smooth muscle strips and dispersed muscle cells, T Kitazawa; S Kikui; T Taneike; A Ohaga, GENERAL PHARMACOLOGY, 27, (4) 655 - 664, 1996 06 |
| RECOVERY OF GASTROINTESTINAL MOTILITY FROM POSTOPERATIVE ILEUS IN DOGS - EFFECTS OF LEU(13)-MOTILIN (KW-5139) AND PROSTAGLANDIN-F2-ALPHA, T YOKOYAMA; T KITAZAWA; K TAKASAKI; A ISHII; A KARASAWA, NEUROGASTROENTEROLOGY AND MOTILITY, 7, (4) 199 - 210, 1995 12 |
| BINDING AND FUNCTIONAL-CHARACTERIZATION OF ALPHA-2-ADRENOCEPTORS IN ISOLATED SWINE MYOMETRIUM, T TANEIKE; T NARITA; T KITAZAWA; S BANDO; H TERAOKA; A OHGA, JOURNAL OF AUTONOMIC PHARMACOLOGY, 15, (2) 93 - 105, 1995 04 |
| MUSCLE LAYER AND REGIONAL DIFFERENCES IN AUTONOMIC INNERVATION AND RESPONSIVENESS TO TRANSMITTER AGENTS IN SWINE MYOMETRIUM, T TANEIKE; SI BANDO; K TAKASAKI; M OKUMURA; H SATO; H TERAOKA; T KITAZAWA; A OHGA, JOURNAL OF AUTONOMIC PHARMACOLOGY, 14, (3) 213 - 227, 1994 06 |
| Enhancement by KW-5092, a Novel Gastroprokinetic Agent, of the Gastrointestinal Motor Activity in Dogs., Kishibayashi Nobuyuki; Tomaru Atsushi; Ichikawa Shunji; Kitazawa Takio; Shuto Katsuichi; Ishii Akio; Karasawa Akira, Journal of Pharmacological Sciences, The Japanese Pharmacological Society, 65, (2) 131 - 142, 1994 |
| Antagonist like action of synthetic α |
Books etc
| Muscarinic regulation of gastrointestinal motility, Tanahashi Yasuyuki; Takio Kitazawa; Toshihiro Unno, Muscarinic receptor From structure to animal models Second edition. 307-339p, Humana Press registered company Springer Science+Business Media, LLC, part of Springer Nature., 2024 07 , 9781071640159 |
| 動物病理学 : 動物薬理学, 日本動物保健看護系大学協会カリキュラム委員会, 動物薬理学 2章の5,6節、3章と4章, インターズー, 2020 01 20 , 9784866710860 |
| 心臓収縮調節に関わる自律神経受容体の再検討―マウス心房筋を用いての解析ー, 北澤多喜雄, MPアグロ, 2019 04 |
| 伴侶動物におけるグレリン研究ー基礎とその臨床応用ー, 北澤多喜雄, 生物科学 69（２）, 生物科学 69（２）農文協, 2018 02 |
| Regulation of heart contractility by M2 and M3 muscarinic receptors: functional studies using muscarinic receptor knockout mouse., Kitazawa T; Teraoka H; Harada N; Ochi K; Nakamura T; Asakawa K; Kanegae S; Yaosaka N; Unno T; Komori S; Yamada M, Chapter 13, Muscarinic receptor. From Structure to Animal Models. Neuromethods Vol. 107. DOI 10.1007/978-1-4939-2858-3_13@Springer Science+Business Media New York. 2015. 235-259, 2015 08 |
| 動物薬理学, 北澤多喜雄, 2,3,4,11章, Interzoo, 2014 10 |
| 獣医薬理学, 北澤多喜雄, 第13章 ホルモン, 近代出版, 2013 03 |
| 薬理学・毒性学実験, 北澤多喜雄, 25-29P, 117-118P, 文英堂, 2008 04 |
Conference Activities & Talks
| Comparative study for regulation of gastrointestinal motiity by motilin, Takio Kitazawa, 156 Annual meeting for Japan veterinary science meeting |
| 北海道東部に生息するタンチョウの糞および腸管内容 物のメタバーコーディング解析：動物食性の検討, 小板 ななみ; 片岡 陽香; 北澤 多喜雄; 飯間 裕子; 吉野 智生; 百瀬 邦和; 中嶋 信美; 遠藤 大二; 寺岡 宏樹, 第163回日本獣医学会学術集会 |
| ネコにおける異物代謝に関わるシトクローム P450分子種の特徴, 尾野 由佳; 岡松 岳; 杉山 颯太; 宇野 泰広; 生城 真一; 北澤 多喜雄; 寺岡 宏樹, 第163回日本獣医学会学術集会 |
| Potentiation of Dioxin-Induced Edema by LPS in Larval Zebrafish, H. Adachi; K. Tanaka; H. Kitamura; T. Kitazawa; H. Teraoka, Society of Toxicology 59th Annual Meeting |
| ゼブラフィッシュ初期発生における肝臓形成前の化学物質代謝, 赤坂 洪暢; 縄司 奨; 山下夏未; 溝口直洋; 安達真里花; 関 雅範; Zhang Shuangy; 北澤多喜雄; 寺岡 宏樹, 野生動物に対するケミカルハザードに関する国際シンポジウム |
| Cytochrome P450s expression and chemical metabolic activity before liver formation in zebrafish eleutheroembryos, 縄司奨; 山下夏未; 溝口直洋; 安達真里花; 関雅範; 北澤多喜雄; 寺岡宏樹, 第42回日本分子生物学会年会 |
| Does motilin regulate gastrointestinal motility of bullfrog? In vitro study uisng isolated GI strips., Kitazawa Takio; Shuangyi Zhang; Teraoka Hiroki; Okuhara Yuji; Kaiya Hiroyuki, NeuroGastro 2019 (Lisobon) |
| Identification of ghrelin and motilin in the pheasant , and their mechanical actions in the isolated gastrointestinal tract., Shuangyi Zhang; Ichiro Sakata; Hiroyuki Kaiya; HirokiTeraoka; Yuji Okuhara; Takio Kitazawa, NeuroGastro 2019 |
| Transcriptional profiling of cytochrome P450 genes in adult liver and in whole embryo fry of zebrafish in some developmental stages, Hiroki Teraoka; Natsumi Yamashita; Yusuke K. Kawai; Shuangyi Zhang; Takio Kitazawa; Akira Kubota, 15th International Congress of Toxicology |
| ゼブラフィッシュ肝臓におけるシトクロムP450分子種のmRNA発現プロファイル, 久保田彰; 川合佑典; 山下夏未; Jae Seung Lee; 近藤大輔; Shuangyi Zhang, 第46回 日本毒性学会学術年会 |
| Alpha1B-adrenoceptor-mediated positive inotropic and positive chronotropic actions in the mouse atrium, Zhang S; Takahashi R; Yamashita N; Teraoka H; Kitazawa T, 92nd Annual Meeting of Japan Pharmacological Society |
| A review of the motilin system in guinea-pig: Re-examination of gastrointestinal motility-stimulating action of guinea-pig motilin candidate in vitro, Kitazawa Takio; Hiroyuki Kaiya, 3rd meeting of the federation of neurogastroenterology and motility |
| Guinea-pig ghrelin, its structure, distribution and function in gastrointestinal trac, Takio Kitazawa; Yuji Okuhara; Hiroyuki Kaiya; Hiroki Teraoka, World Clinical Pharmacology 2018 Kyoto |
| Effects of prostaglandin E2 and F2 α on expression of growth factors and pro-, Shuangyi Zhang; Kitazawa Takio; Jinshan Cao, World Clinical Pharmacology 2018 Kyoto |
| ダイオキシン毒性と炎症のクロストーク, 田中勝貴; 二条久保大祐; 安達 光; 北澤多喜雄; 寺岡宏樹, 2017年度生命科学系学会合同年次大会（ConBio2017） |
| 犬血漿中アミノ酸量のバランス変化による腫瘍診断法の確立, 猿渡菜美香; 番目北澤多, 第16回日本獣医学会学術集会 |
| 発達期ゼブラフィッシュにおけるPCBおよび臭素化難燃物による自発運動の増強：ドーパミン神経経路とメラニン合成経路の関与, 田中泰彰; 藤原真理; 北澤多喜雄; 寺岡宏樹, 第44回日本毒性学会学術年会 |
| Chicken is a unique animal model for studying function of ghrelin in gastrointestinal motility, Kitazawa Takio, International Symposium on Ghrelin and Energy metabolism Homeostasis |
| Vascular Endothelium Is A Target Of 2,3,7,8-tetrachlorodibenzo-p-dioxin In Cardiac Edema In Early Zebrafish Larvae, 二条久保大祐; 田中勝貴; 田中泰彰; 北澤多喜雄; 寺岡宏樹, 第56回全米トキシコロジー学会 |
| ムスカリン受容体を介した胃粘膜細胞の分化・増殖制御, 松山勇人; 内藤清惟; 和田善明; 永野宏; 斉藤正一郎; 酒井洋樹; 棚橋靖行; 北澤多喜雄; 小森成一; 海野年弘, 第159回日本獣医学会学術集会 |
| Possible regulation of gastrointestinal motility of zebrafish by motilin: An invitro study using isolated muscle strips, Kitazawa Takio, RegPep2016 |
| What is the general action of ghrelin invertebrates? Study focused on gastrointestinal motor stimulating action, Kitazawa Takio, 第93回日本生理学会 |
Works
| 脊髄のカテコールアミン反応性, 北澤多喜雄, 1979 04 - 1981 03 , 北海道大学大学院修士課程での仕事 修士論文の内容は以下に公表した。 Effects of catecholamines on spinal motoneurones and spinal reflex discharges in the isolated spinal cord of the newborn rat. Kitazawa T, Saito K, Ohga A. Brain Res. 1985 Mar;351(1):31-6. |
Misc
| Does oxytocin directly regulate the atrial contractility in mouse?, Makoto Ito; Takio Kitazawa, J.Rakuno Gakuen Univ., 49, (2) 101 - 109, 2025 03 |
| Is motilin a physiological regulator of rabbit gastrointestinal motility?, Kitazawa Takio; Zhang Shuangyi, J.Rakuno Gakuen Univ., 49, (2) 81 - 99, 2025 03 |
| Physiological roles of muscarinic acetylcholine receptor in the gastrointestinal tract: Functional study using muscarinic receptor knockout mice, Kitazawa Takio, . Rakuno Gakuen Univ., 48 (2)：255～276 (2024), 48, (2) 255 - 276, 2024 03 |
| Comparative study for motilin in vertebrates; its structure, distribution, receptor and gastrointestinal motility stimulating action, Takio Kitazawa; Shuangyi Zhang; Hiroyuki Kaiya, J. Rakuno Gakuen Univ.,, 47, (2) 149 - 177, 2023 03 |
| Ghrelin in vertebrates; distribution, receptor, release mechanism and regulation of gastrointestinal motility, Takio KITAZAWA; Shuangyi ZHANG; Hiroyuki KAIYA, J. Rakuno Gakuen Univ., 47 (2)：179～197 (2023), 47, (2) 179 - 197, 2023 03 |
| Smooth muscle layer- and region-dependent differences in autonomic innervation and responsiveness to bioactive substances in the porcine uterus, Takio KITAZAWA; Tetsuro TANEIKE; Jinshan CAO, J. Rakuno Gakuen Univ.,, 46, (2) 15 - 27, 2022 03 10 |
| Regulation of cardiac contractility by autonomic nerves and their neurotransmitters in the isolated mouse atrium, 北 澤 多喜雄; 浅 川 幸 一; 原 田 奈 央; 越 智 建 太; 高 橋 礼 奈; Zhang SHUANGYI, J. Rakuno Gakuen Univ.,, 46, (2) 1 - 13, 2022 03 10 |
| ネコの膀胱炎から分離された多剤耐性大腸菌に対する新規ファージの分離とその性状解析, 大橋春香; 大橋春香; 中村暢宏; 酒井俊和; 藤木純平; 北澤多喜雄; 村田亮; 岩崎智仁; 岩野英知, 日本獣医学会学術集会講演要旨集, 164th (CD-ROM), 2021 |
| 発達期ゼブラフィッシュのAHR2介在性浮腫における炎症および酸化ストレスの関与, 田中 勝貴; 安達 光; 二条久保 大祐; 北澤 多喜雄; 寺岡 宏樹, The Journal of Toxicological Sciences, 43, (Suppl.) S327 - S327, 2018 06 |
| ダイオキシン毒性と炎症のクロストーク, 田中 勝貴; 二条久保 大祐; 安達 光; 北澤 多喜雄; 寺岡 宏樹, 生命科学系学会合同年次大会, 2017年度, 2017 12 |
| 犬血漿中アミノ酸量のバランス変化による腫瘍診断法の確立, 猿渡 菜美香; 樋口 豪紀; 北澤 多喜雄; 福本 真也; 藤木 純平; 打出 毅; 遠藤 能史; 廉澤 剛; 井上 博紀; 岩野 英知, 日本獣医学会学術集会講演要旨集, 160回, 2017 08 |
| マウス結腸輪走筋のコリン作動性神経-平滑筋伝達におけるM2およびM3ムスカリン受容体サブタイプの役割, 松山 勇人; 佐久間 絵光; 永野 宏; 棚橋 靖行; 北澤 多喜雄; 海野 年弘, 日本獣医学会学術集会講演要旨集, 160回, 2017 08 |
| 動物看護学課程で提供される望ましい野生動物(医)学教育とは―酪農学園大学獣医学群における事例から―, 浅川満彦; 郡山尚紀; 佐野忠士; 北澤多喜雄, Veterinary Nursing, 22, (1) 43 - 43, 2017 07 01 |
| イヌ血液中アミノ酸量の変化に基づいた腫瘍診断法の確立, 猿渡 菜美香; 大野 さや; 北澤 多喜雄; 井上 博紀; 福本 真也; 打出 毅; 遠藤 能史; 廉澤 剛; 岩野 英知, Veterinary Nursing, 22, (1) 29 - 29, 2017 07 |
| 発達期ゼフラフィッシュにおけるPCBおよび臭素化難燃物による自発運動の増強 ドーパミン神経経路とメラニン合成経路の関与, 田中 泰彰; 藤原 麻里; 北澤 多喜雄; 寺岡 宏樹, The Journal of Toxicological Sciences, 42, (Suppl.) S282 - S282, 2017 06 |
| 犬血漿中アミノ酸量のバランス変化による腫瘍診断法の確立, 岩野英知; 猿渡菜美香; 大野さや; 樋口豪紀; 北澤多喜雄; 福本真也; 打出毅; 遠藤能史; 廉澤剛; 横田博, 北海道獣医師会雑誌, 60, (8) 406 - 406, 2016 08 10 |
| タンチョウの水銀蓄積特性に関する検討, 寺岡宏樹; 岡本絵梨佳; 久道萌; 中山翔太; 池中良徳; 石塚真由美; 北澤多喜雄, 環境化学討論会要旨集(CD-ROM), 24th, 2015 06 17 |
| ネコ、イヌ、ヒトにおけるシトクロームP450 3A分子種の動物種差, 岡松 岳; 川上 圭; 小松 徹也; 宇野 泰広; 北澤 多喜雄; 寺岡 宏樹, 中毒研究, 27, (3) 279 - 279, 2014 09 |
| CHICKEN IS A SPECIFIC AND USEFUL ANIMAL MODEL TO STUDY THE FUNCTIONAL ROLE OF GHRELIN AND MOTILIN IN REGULATION OF GASTROINTESTINAL MOTILITY, T. Kitazawa; H. Teraoka; H. Kaiya, JOURNAL OF MOLECULAR NEUROSCIENCE, 53, 2014 08 |
| Involvement of M-2 or M-3 muscarinic receptor-coupled signalling molecules in the activation of muscarinic cation channels in mouse ileal smooth muscle cells, Toshihiro Unno; Taisuke Katsurada; Hayato Matsuyama; Yasuyuki Tanahashi; Takio Kitazawa; Masahisa Yamada; Yurgen Wess, JOURNAL OF PHARMACOLOGICAL SCIENCES, 121, 2013 |
| Muscarinic regulation of ATP sensitive K+ channels in mouse ileal smooth muscles, Yasuyuki Tanahashi; Toshihiro Unno; Hayato Matsuyama; Takio Kitazawa; Masahisa Yamada; Jurgen Wess; Komori Seiichi, JOURNAL OF PHARMACOLOGICAL SCIENCES, 121, 2013 |
| Basic biochemical study for growth hormone secretagogue-receptor (GHS-R) 1a and plasma ghrelin in dogs, KITAZAWA Takio; ISHIDA Shoji; IWA Masaru; YAMAGATA Tomoya; OCHI Kenta; UCHIDA Eiji; HIRAGA Takeo; KAIYA Hiroyuki, Journal of the College of Dairying. Natural science, 36, (2) 295 - 310, 2012 04 |
| A ganglion stimulant, 1, 1dimethyl-4phenylpiperazinium iodide(DMPP) stimulates both cholinergic and adrenergic neurons in isolated atrium of the mouse, Kenta Ochi; Hiroki Teraoka; Toshihiro Unno; Sei-ichi Komori; Masahisa Yamada; Takio Kitazawa, JOURNAL OF PHARMACOLOGICAL SCIENCES, 118, 2012 |
| Neural M1 muscarinic receptor mediates inhibition of mouse colonic motility through activation of nitrergic nerves, Takaji Kondo; Hiroki Teraoka; Toshihiro Unno; Sei-ichi Komori; Masahisa Yamada; Takio Kitazawa, JOURNAL OF PHARMACOLOGICAL SCIENCES, 118, 2012 |
| 北海道におけるタンチョウの水銀汚染の原因についての検討, 岡本絵梨佳; 久道萌; 水野直治; 古賀公也; 志村良治; 中山翔; 池中良徳; 石塚真由美; 北澤多喜雄; 井上雅子; 百瀬邦和; 正富宏之; 寺岡宏樹; 寺岡宏樹, 日本鳥学会大会講演要旨集, 2011, 2011 09 13 |
| 北海道におけるタンチョウの水銀汚染の原因についての検討, 岡本絵梨佳; 久道萌; 水野直治; 古賀公也; 志村良治; 中山翔; 池中良徳; 石塚真由美; 北澤多喜雄; 井上雅子; 百瀬邦和; 正富宏之; 寺岡宏樹, 日本鳥学会大会講演要旨集, 2011, 2011 09 13 |
| Role of M3 muscarinic receptor-coupled signalling molecule in the activation of muscarinic cation channels in mouse ileal smooth muscle cells, Toshihiro Unno; Michiko Inasaki; Hayato Matsuyama; Yasuyuki Tanahashi; Takio Kitazawa; Masahisa Yamada; Yurgen Wess; Seiichi Komori, JOURNAL OF PHARMACOLOGICAL SCIENCES, 115, 2011 |
| 脊椎動物共通プライマーを用いた大規模ディファレンシャル・ディスプレイ法によるダイオキシン応答遺伝子の探索, 鵜飼 典佳; 遠藤 大二; 池田 晴喜; 伊藤 知子; 伊東 志野; 金 恩英; 岩田 久人; 北澤 多喜雄; 平賀 武夫; 寺岡 宏樹, 日本獣医学会学術集会講演要旨集, 150回, 2010 09 |
| エコトキシコゲノミクスへの応用を目的とした半網羅的ディファレンシャル・ディスプレイ法, 伊東 志野; 遠藤 大二; 池田 晴喜; 伊藤 知子; 鵜飼 典佳; 金 恩英; 岩田 久人; 北澤 多喜雄; 平賀 武夫; 寺岡 宏樹, The Journal of Toxicological Sciences, 35, (Suppl.) S181 - S181, 2010 05 |
| Region-related contractile response to tetrodotoxin in mouse gastrointestinal tract, Yuichi Okuno; Takio Kitazawa, JOURNAL OF PHARMACOLOGICAL SCIENCES, 112, 2010 |
| Ghrelin Stimulates Gastric Motility Through Activation of the Vago-Vagal Reflex Pathway in the Anesthetized Guinea-Pig, Takio Kitazawa; Takenori Onaga; Tatsuro Nakamura, GASTROENTEROLOGY, 136, (5) A534 - A534, 2009 05 |
| Opposite inotropic action induced by activation of M2 and M3 muscarinic receptors in mouse atrium, Kitazawa Takio; Asakawa Koichi; Teraoka Hiroki; Unno Toshihiro; Komori Sei-ichi; Yamada Masahisa; Wess Jurgen, Journal of pharmacological sciences, 109, (1) 86 - 86, 2009 03 |
| Ghrelin stimulates gastric motility of the anesthetized guinea-pig, Nakamura Tatsuro; Onaga Takenori; Kitazawa Takio, Journal of pharmacological sciences, 109, (1) 181 - 181, 2009 03 |
| Ghrelin stimulates gastric motility of the anesthetized guinea-pig, Tatsuro Nakamura; Takenori Onaga; Takio Kitazawa, JOURNAL OF PHARMACOLOGICAL SCIENCES, 109, 2009 |
| Opposite inotropic action induced by activation of M2 and M3 muscarinic receptors in mouse atrium, Takio Kitazawa; Koichi Asakawa; Hiroki Teraoka; Toshihiro Unno; Sei-ichi Komori; Masahisa Yamada; Jurgen Wess, JOURNAL OF PHARMACOLOGICAL SCIENCES, 109, 2009 |
| Activation of cationic channels by diacylglycerol in mouse ileal smooth muscle cells, Sakamoto Takashi; Unno Toshihiro; Matsuyama Hayato; Kitazawa Takio; Taneike Tetsuro; Yamada Masahisa; Wess Jurgen; Komori Seiichi, Journal of pharmacological sciences, 106, (1) 270 - 270, 2008 02 |
| M3 muscarinic receptor mediates carbachol-induced contraction of mouse uterine smooth muscle, Kitazawa Takio; Hirama Ryuichi; Masunaga Kozue; Nakamura Tatsuro; Asakawa Koichi; Cao Jinshan; Teraoka Hiroki; Unno Toshihiro; Komori Sei-ichi; Yamada Masahisa; Wess Jurgen; Taneike Tetsuro, Journal of pharmacological sciences, 106, (1) 108 - 108, 2008 02 |
| Activation of cationic channels by diacylglycerol in mouse ileal smooth muscle cells, Takashi Sakamoto; Toshihiro Unno; Hayato Matsuyama; Takio Kitazawa; Tetsuro Taneike; Masahisa Yamada; Jurgen Wess; Seiichi Komori, JOURNAL OF PHARMACOLOGICAL SCIENCES, 106, 2008 |
| M3 muscarinic receptor mediates carbachol-induced contraction of mouse uterine smooth muscle, Takio Kitazawa; Ryuichi Hirama; Kozue Masunaga; Tatsuro Nakamura; Koichi Asakawa; Jinshan Cao; Hiroki Teraoka; Toshihiro Unno; Sei-ichi Komori; Masahisa Yamada; Jurgen Wess; Tetsurci Taneike, JOURNAL OF PHARMACOLOGICAL SCIENCES, 106, 2008 |
| Three distinct muscarinic signaling pathways for cationic channel activation in gut smooth muscle cells, Seiichi Komori; Takashi Sakamoto; Toshihiro Unno; Takio Kitazawa; Tetsuro Taneike; Masahisa Yamada; Jurgen Wess, NEUROGASTROENTEROLOGY AND MOTILITY, 19, 2007 10 |
| Ghrelin-induced contractions and growth hormone secretagogue receptor expression in the chicken gastrointestinal tract, Takio Kitazawa; Hiroyuki Kaiya; Tetsuro Taneike, GASTROENTEROLOGY, 132, (4) A542 - A542, 2007 04 |
| Synergistic activation of receptoroperated cationic channels by M-2 and M-3 muscarinic receptors in mouse ileal smooth muscle cells., Takashi Sakamoto; Toshihiro Unno; Hayato Matsuyama; Takio Kitazawa; Tetsuro Taneike; Masahisa Yamada; Wess Jurgen; Seiichi Komori, JOURNAL OF PHARMACOLOGICAL SCIENCES, 103, 2007 |
| Different functional roles of M2 and M3 receptors in gastric contraction induced by carbachol and nerve stimulation, Takio Kitazawa; Kano Hashiba; Jinshan Cao; Toshihiro Unno; Sei-Ichi Komori; Masahisa Yamada; Jurgen Wess; Tetsuro Taneike, JOURNAL OF PHARMACOLOGICAL SCIENCES, 103, 2007 |
| Characterization of prostanoid receptors present on adrenergic neurons of porcine uterine longitudinal muscle, Jinshan Cao; Takio Kitazawa; Tetsuro Taneike, JOURNAL OF PHARMACOLOGICAL SCIENCES, 103, 2007 |
| Gastrointestinal-motility stimulating action of ghrelin in isolated chicken gastrointestinal tract, Takio Kitazawa; Tetsuro Taneike, ACTA PHARMACOLOGICA SINICA, 27, 2006 07 |
| Endogenous prostaglandins (PGs) regulate spontaneous contractility of non-pregnant porcine myometrium, Jinshan Cao; Takio Kitazawa; Tetsuro Taneike, ACTA PHARMACOLOGICA SINICA, 27, 2006 07 |
| Effects of ghrelin peptides on contractility of isolated chicken gastrointestinal tracts, T Kitazawa; T Taneike, JOURNAL OF PHARMACOLOGICAL SCIENCES, 100, 2006 |
| モチリン・アンタゴニストの同時投与がGHSアゴニストの摂食抑制作用に及ぼす影響, カーン シャキル.イスラム; 百々功一; 上田博史; 種池哲朗; 北沢多喜雄; 保坂善真; 高梨契典; 豊後貴嗣, 日本家禽学会誌, 42, 2005 10 15 |
| PHARMACOLOGICAL STUDY FOR CHARACTERIZATION OF THE NON-ADRENERGIC, NON-CHOLINERGIC (NANC) NEUROTRANSMITTER IN THE BOVINE UTERUS, KITAZAWA Takio; HATAKEYAMA Hirofumi; ONODERA Shuichi; SHIOMI Norio; TANEIKE Tetsuro, 日本平滑筋学会機関誌, 9, (2) "J - 47"-"J-64", 2005 08 01 , Pharmacological characteristics of electrical field stimulation (EFS)-induced mechanical responses in the bovine uterus indicate smooth muscle layer-specific variations in autonomic innervation (exclusive adrenergic innervation in the longitudinal muscle and non-adrenergic, non-cholinergic (NANC) innervation in the circular muscle). The aim of the present study was to characterize the neurotransmitter of NANC nerves in the bovine uterus using functional and biochemical approaches. EFS of uterine circular muscle caused atropine and guanethidine-resistant contraction, which was abolished by tetrodotoxin. The EFS-induced contraction was not decreased by pyrilamine, methysergide or indomethacin. In the presence of atropine and guanethidine, bioactive peptides (angiotensin II, bombesin, calcitonin-gene related peptide, enkephalins, galanin, neurokinin A, neurokinin B, substance P, neurotensin, vasoactive intestinal polypeptide), ATP and 5-hydroxytryptamine did not produce contraction of the myometrium. An acid extract of the bovine uterus (EBU) contracted both guinea-pig ileum longitudinal muscle (GPILM) and bovine uterus. Neither tetrodotoxin nor methysergide decreased the EBU-induced contraction. Atropine and pyrilamine inhibited the EBU-induced responses, but some of the contractions (5-15%) were resistant to both antagonists. On a Sephadex G50 column, smooth muscle contracting material was eluted in one peak. Active fractions were pooled and applied to a Sephadex G25 column to compare the elution volume of the active material with those of peptides of known molecular weights. The active material was eluted in one peak and the molecular weight was estimated to be 1200. The gel-filtrated fraction induced contraction of both GPILM and bovine uterus, and its contractile activity was abolished by α-chymotrypsin. In conclusion, uterotonic peptide was discovered in extract of the bovine uterus and was thought to be one of transmitter for NANC nerves. However, further studies are needed to clarify the structure of this unknown peptide and its physiological roles. |
| GHS‐R AgonistおよびAntagonist投与がニワトリヒナの摂食量,消化管通過に及ぼす影響, 百々功一; 上田博史; 種池哲朗; 保坂善真; 北沢多喜雄; 豊後貴嗣, 日本家禽学会誌, 42, 2005 03 10 |
| Involvement of the endogenous prostaglandins in regulation of spontaneous contraction in non-pregnant porcine uterus, JS Cao; T Kitazawa; T Taneike, JOURNAL OF PHARMACOLOGICAL SCIENCES, 97, 2005 |
| Distribution and functional roles of 5-HT2A and 5-HT7 receptors in porcine uterus, T Iwamoto; T Kitazawa; JS Cao; T Taneike, JOURNAL OF PHARMACOLOGICAL SCIENCES, 97, 2005 |
| In vivo and in vitro effects of peptide and nonpeptide ghrelin receptor agonists on gastric emptying in mice, Depoortere, I; T Kitazawa; B De Smet; T Peeters, REGULATORY PEPTIDES, 122, (1) 15 - 15, 2004 09 |
| Region-related distribution of prostanoid receptors in non-pregnant porcine uterus, JS Cao; T Kitazawa; T Taneike, JOURNAL OF PHARMACOLOGICAL SCIENCES, 94, 2004 |
| Gastroprokinetic action of growth hormone secretagogue receptor (GHS-R) agonists in mice, T Kitazawa; T Taneike; B De Smet; Depoortere, I; T Peeters, JOURNAL OF PHARMACOLOGICAL SCIENCES, 94, 2004 |
| Presence of thromboxane A(2) (TP) receptor in the non-pregnant porcine uterus., JS Cao; T Kitazawa; T Taneike, JOURNAL OF PHARMACOLOGICAL SCIENCES, 91, 2003 |
| ブタ子宮に存在する収縮性及び弛緩性EP受容体subtypeの解析, 曹 金山; 田島 剛; 沙衣布扎提; 北澤 多喜雄; 種池 哲朗, 日本獣医学会学術集会講演要旨集, 133回, 2002 03 |
| Potentiation of motilin-induced contraction by nitric oxide synthase inhibition in the isolated chicken gastrointestinal tract, T Kitazawa; C Onodera; T Taneike, NEUROGASTROENTEROLOGY AND MOTILITY, 14, (1) 3 - 13, 2002 02 , 10.1046/j.1365-2982.2002.00298.x |
| Subtype of prostanoid EP receptors present in the non-pregnant porcine myometrium, T Kitazawa; M Shayibuzhati; T Tajima; JS Cao; T Taneike, JAPANESE JOURNAL OF PHARMACOLOGY, 88, 2002 |
| ブタ子宮におけるprostanoid受容体発現の筋層差, 田島 剛; 北澤 多喜雄; ミクラム・サイブザティ; 種池 哲朗, 日本獣医学会学術集会講演要旨集, 132回, 2001 09 |
| ブタ子宮筋に存在するプロスタノイド受容体サブタイプの薬理学的解析, 沙衣布扎堤; 米克熱木; 畠山 洋文; 田島 剛; 北澤 多喜雄; 種池 哲朗, Journal of Smooth Muscle Research Japanese Section, 5, (2) J69 - J80, 2001 08 , 10.1540/heikatsukinzashi1997.5.J69 |
| ブタ子宮におけるプロスタノイド受容体分布の筋層差, 北澤 多喜雄; 沙衣布扎提; 木; 田島 剛; 種池 哲朗, Journal of Smooth Muscle Research Japanese Section, 5, (1) J47 - J47, 2001 04 |
| ブタ子宮筋のacetylcholine誘発性収縮に関与するmuscarine受容体subtypeの解明, 種池 哲朗; 内山 史一; 広瀬 佳代; 北澤 多喜雄, Journal of smooth muscle research. Japanese section, 2, (1) J - 49, 1998 04 30 |
| Histamine mediates the muscle layer-specific responses in the isolated swine myometrium, T Kitazawa; H Shishido; T Sato; T Taneike, JOURNAL OF VETERINARY PHARMACOLOGY AND THERAPEUTICS, 20, (3) 187 - 197, 1997 06 , 10.1111/j.1365-2885.1997.tb00094.x |
| EXCITATORY ACTION OF [LEU(13)]MOTILIN ON THE GASTROINTESTINAL SMOOTH-MUSCLE ISOLATED FROM THE CHICKEN, T KITAZAWA; T TANEIKE; A OHGA, PEPTIDES, 16, (7) 1243 - 1252, 1995 , 10.1016/0196-9781(95)00095-2 |
| THE LEU(13)-MOTILIN (KW-5139)-EVOKED RELEASE OF ACETYLCHOLINE FROM ENTERIC NEURONS IN THE RABBIT DUODENUM, T KITAZAWA; A ISHII; K TANIYAMA, BRITISH JOURNAL OF PHARMACOLOGY, 109, (1) 94 - 99, 1993 05 , 10.1111/j.1476-5381.1993.tb13536.x |
Research Grants & Projects
| 文部科学省, 科学研究費 基盤研究C, グレリン―モチリン系による消化管運動調節機構に関する比較生物学的研究, 201404, 201703, 北澤多喜雄 |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Role of ATP sensitive K+ channels in muscarinic regulation of intestinal contractility and its molecular identity, 201304, 201603, TANAHASHI Yasuyuki; WESS Jurgen; YAMADA Masahisa; KITAZAWA Takio; UNNO Toshihiro; KOMORI Seiichi; MATSUYAMA Hayato; NAGANO Hiroshi; TAKEUCHI Minoru; MURAKAMI Yuri; WANG Ban; ONO Maiki, The aim of this study was to elucidate the physiological roles of ATP-sensitive K+ channels (KATP channels) in muscarinic regulation of intestinal contractility and its mechanism. In addition, the properties of this channel were characterised. The results suggested that (1) the properties of the KATP channel, expressed in the intestinal smooth muscles, are similar to those of the Kir6.1/ SUR2B KATP channel in the vascular smooth muscles, and (2) M3 muscarinic subtype can inhibit the activities of the KATP channel, thereby contributing to the membrane depolarisation in smooth muscles, leading to their contraction. |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Age-related changes in ghrelin function, ghrelin concentration and ghrelin related molecules expression in the growing chickens., 201104, 201403, KITAZAWA Takio; KAIYA Hiroyuki; SAKAI Takafumi, Ghrelin contracted a chicken gastrointestinal tract. The ghrelin-induced contraction and expression of growth hormone secretagogue receptor (GHS-R1a) mRNA in the proventriculus decreased depending on age. Ghrelin concentrations in the proventriculus and plasma increased after hatching and reached plateau at 30 days. Expression of ghrelin and ghrelin-O-acetyltransferase (GOAT) mRNAs also tended to decrease depending on age. Expression of ghrelin mRNA did not show any correlation with plasma ghrelin. Inverse relationships between ghrelin concentration and GHS-R1a and GOAT mRNAs expression are indicative for a ghrelin-induced downregulation of the ghrelin receptor and synthetic enzyme. The expression pattern of ghrelin, GOAT and GHS-R1a mRNAs in the brain distinctly change in region-dependent manner on growing stage. The present results indicated that physiological role of ghrelin system changes in the brain and gastrointestinal tract of growing chickens. |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Elucidation of mechanisms for the generation of gut peristaltic reflex using various types of knockout mice., 201004, 201303, KOMORI Seiichi; UNNO Toshihiro; KITAZAWA Takio; IINO Satoshi; MATUYAMA Hayato; TANAHASHI Yasuyuki; JURGEN Wess; YAMADA Masahisa; SAKAI Hiroki; SAITOH Shouchirou; ANDOH Kumiko; KIMURA Kaori; SUGIYAMA Kyoko; MATSUI Ryuusuke; KATSURADA Taisuke; NAITO Kiyiotada; SOBUE Yuki; NAGANO Hiroshi; WAKATA Tomohiro, The cholinergic neurons play an important rolein the generation and regulation of gut peristaltic reflex. Acetylcholine released from the cholinergic neurons act on muscarinic receptors in various neurons and smooth muscles to produce peristalsis. However, the precise roles of muscarinic receptor subtypes have not yet been elucidated. In the present study, we analyzed gut peristalsis in muscarinic receptor subtype knockout mice. The results suggest that M2 subtype of muscarinic receptor plays an essential role in the generation of peristalsis and that this subtype may be a useful target for the development of new drugs to treat for various intestinal disorders, such as irritable bowel syndrome. |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Identification of novel ghrelin receptor in fish and clarification of the relationship between the receptor and biological action., 2008, 2010, KAIYA Hiroyuki; MIYAZATO Mikiya; KANGAWA Kenji; MATSUDA Kouhei; KITAZAWA Takio, In this study, we cloned cDNAs for four functional ghrelin receptors (GHS-R1a-1, 1a-2, 2a-1 and 2a-2) that composed of 360, 360, 366 and 367 amino acids, respectively in goldfish brain. Each receptor mRNA showed tissue-specific expression, suggesting that each receptor mediates tissue-specific action of ghrelin in goldfish. One species of ghrelin receptor, GHS-R1a has been known to be present in vertebrates other than fish examined so far. We tried to isolate new ligands for the new functional GHS-Ra, which is the novel type of ghrelin receptor, from tissue extracts of goldfish brain and intestine, but unfortunately the new ligand was not able to find out during the course of this study. |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Ghrelin peptides induced contractions and growth hormone secretagogue receptor expression in the chicken gastrointestinal tract, 200404, 200703, KITAZAWA Takio; ONAGA Takenori; IWANO Hidetomc, Background and aim. Ghrelin is the endogenous ligand for the growth hormone secretagogue receptor (GHS-R), and stimulates food intake and gastrointestinal (GI) motility in mammals. In chickens, on the other hand, ghrelin inhibits food intake. This discrepancy prompted us to investigate whether ghrelin affects gastrointestinal motility in the chicken. Methods, The contractile effect of chicken ghrelin was measured isometrically in muscle strips from different regions of the GI tract suspended in an organ bath. The effect of chicken ghrelin was compared with that of rat or human ghrelin. In addition, mRNA expression of ghrelin and GHS-R was examined by quantitative RT-PCR. Results. Among the ghrelin peptides tested (rat, human and chicken ghrelin), only chicken ghrelin caused contraction of muscle strips from the GI tract. No effect was seen in des-octanoylated chicken ghrelin, suggesting that octanoylation at Ser^3 residue was essential for its activity. The contractile response to chicken ghrelin in the crop was not affected by tetrodotoxin (TTX), but that in the proventriculus was decreased by TTX and atropine to the same extent. D-Lys^3-GHRP-6 (a GHS-R antagonist) caused a transient contraction and inhibited the chicken ghrelin-induced contraction without affecting the 50 mM high-K^+-induced contraction. The amplitude of the chicken ghrelin-induced contraction was different among GI regions: highest in the crop and colon (70% of high-K^+-induced contraction), moderate in the esophagus and proventriculus (30%), and weak in the small intestine (10-20%) and the ranking order was consistent with GHS-R mRNA expression in each region (colon > crop = esophagus > proventriculus > duodenum =jejunum > ileum). GHS-R mRNA expression in the muscle preparations of crop and proventriculus without mucosa was 2-4 times higher than that in whole segments of crop and proventriculus, indicating that the GHS-R is mainly expressed in smooth muscle layers including enteric nerves. In contrast to the GHS-R expression, ghrelin mRNA was mainly expressed in the mucosa of proventriculus and duodenum. Conclusion. These results indicate that ghrelin stimulates GI contractility and preferentially affects the motor activity of esophagus, crop and colon through myogenic and/or neurogenic mechanisms. These actions are mediated through the GHS-R, which is highly sensitive to chicken ghrelin |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Pharmacological characterization of prostanoid receptors present in the porcine uterus, 200104, 200403, TANEIKE Tetsuro; KITAZAWA Takio; YOKOTA Hiroshi, In the present research project, prostanoid receptor subtype and distribution of cyclooxygenase (COX) in the porcine uterus were characterized using pharmacological and biochemical techniques, and physiological roles of prostanoid in regulation of myometrial contractility were discussed. Contractile (EP1, EP3, FP and TP) and relaxant prostanoid receptors (DP, IP and EP2) were demonstrated in the porcine uterus muscle and these receptor populations were consistent with human. Therefore the porcine uterus might be a useful animal model for assessing the mechanical actions of prostanoid receptor agonists in the human uterus. Prostanoid receptors in the porcine uterus was suggested to distribute heterogeneously depending on smooth muscle types (longitudinal and circular muscle) and uterine regions (cornu, corpus and cervix). In the longitudinal muscle, responsiveness to contractile prostanoid receptor agonists was high compared with the circular muscle and decreased gradually from cornu to cervix. In contrary, responsiveness to relaxant prostanoid receptor agonists was low in the cornu and increased toward cervix. On the other hand, although responses to relaxant prostanoid receptor agonists were high in the circular muscle, regional difference in responsiveness to both contractile and relaxant prostanoid receptor agonists was not marked in comparison with the case of longitudinal muscle. The heterogeneous distribution of contractile and relaxant prostanoid receptor contributes to produce a gradient of the intra-luminal pressure from the cornu (high) to cervix (low) that is convenient for transportation of luminal contents. COX-1 but not COX-2 was expressed in the non-pregnant porcine uterus and distributed heterogeneously depending on muscle layer and uterine regions as did contractile prostanoid receptors. Inhibitors of COX and thromboxane A2 synthase decreased the spontaneous contraction of longitudinal muscle but not circular muscle. Present results suggest that prostanoid modulates, spontaneous myometrial contractility in pig. In the uterus of pregnant pig, COX-2 was expressed intensely and the responsiveness to a FP receptor agonist increased, suggesting a pregnancy-associated change in the physiological role of COX-prostanoid pathway. Further examinations might be necessary to clarify how steroid hormones regulate prostanoid receptor and COX expression, and myometrial motility. |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Charactrristics of motilin-induced contraction and its developmental change in the chicken gastrointestinal tract, 199904, 200203, KITAZAWA Takio; TAKEHARA Kazushige; YOKOTA Hiroshi, Chicken motilin (ch-MT) caused contractions of longitudinal muscle strips of the chicken stomach (proventriculus, Prov) though both myogenic and neurogenic (mostly cholinergic) mechanisms. On the other hand, ch-MT contracted the small intestine through only a myogenic mechanism. The sensitivity of gastrointestinal tracts to ch-MT was region-dependent (jejunum > ileum > duodenum > proventricculus > colon). Ch-MT also stimulated the gastric motility in vivo. The present experiments were designed to investigate developmental changes in responsiveness to ch-MT and in characteristics of electrical field stimulation (EFS)-induced responses, and interaction between NO and ch-MT. Immunohistochemical study was also carried out to examine the presence of ch-MT containing cells. Development of ch-MT-induced contraction : Amplitude of the ch-MT-induced contraction not-log EC_<50> value decreased gradually depending on hatching days(1-50 days), because of the reduction of indirect contraction through cholinergic nerves. But response to a Ach and 5-HT in Prov, and ch-MT-induced contraction in the ileum were not changed at every hatching day. These result suggest the physiological roles of MT in the chicken small intestinal motility ch-MT and NO interaction : L-Nitroarginine methyleaster (L-NAME) potentiated and L-arginine inhibited ch-MT and EFS-induced contractions in Prov without affecting the response to Ach and 5-HT. However, in the ileum and atropine-treated Prov, neither L-NAME nor L-arginine modified the responses to ch-MT. NO synthase inhibition potentiated the contractile response of ch-MT and EFS in Prov through reduction of endogenous NO-mediated presynaptic inhibition on neural ACh release. However, NOS inhibition did nor modify the myogenic (direct) action of ch-MT in gastric and intestinal smooth muscles. Innervation of chicken Prov : Prov was innervated 5 types of autonomic nerves, such as cholinergic excitatory nerve (0.1 - 20 Hz), nonadrenergicnoncholinergic (NANC) excitatory nerves (0.5 - 1 Hz and over 10Hz), NO inhibitory nerve (2 - 7 Hz), NANC, nonNO inhibitory nerve (over 7 Hz). Cholinergic and NO innervation was already completed on 1 day after hatching but NANC, nonNO inhibitory nerve was slowly. Immunohisyochemistry : Specific ch-MT antibody was produced using rabbits. MT-immunoreactive cells were present in the pylorus, duodenum, jejunum, ileum but not in the gizzard, Prov, rectum and caecum. MT containing cell present regions had a high sensitivity to ch-MT compared with other regions. MT is a motor stimulating hormone in the chicken intestinal tracts. |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Functional study for physiological role of 5-hydroxytryptamine7 receptors in porcine myometrium, 199804, 200103, TANEIKE Tetsuro; KITAZAWA Takio; YOKOTA Hiroshi, 5-Hydroxytryptamine (5-HT) inhibits the spontaneous contraction in longitudinal (LM) and circular muscles (CM) of the porcine uterus but CM is more sensitive to 5-HT than LM.The present experiments were designed to clarify the 5-HT receptor subtype mediating the inhibition, subcellular mechanisms of the inhibition and mechanisms underlying the muscle layer-dependent inhibition by 5-HT Receptor subtype : On the basis of ranking order of 5-HT receptor agonists (5-carboxamidotryptamine>5-HT>5-methoxytryptamine>8-hydroxy-2-(di-n-propylamino) tetralin) and 5-HT receptor antagonists (methiothepin>methysergide>metergoline>mianserin>clozapine>spiperone), it was demonstrated that the 5-HT_7 receptor mediated the inhibition of myometrial contractility by 5-HT. Inhibitory mechanism : Analysis of the 5-HT-induced inhibition suggested a following cascade of events in the porcine myometrium : 5-HT_7 receptor activation→activation of Gs→activation of adenylate cyclase→increase in cAMP→decrease in [Ca^<2+>]i and Ca^<2+>-sensitivity of the contractile elements→inhibition of muscle contractility. 5-HT_7receptor distribution : LM and CM layers contained a single class of [^3H]5-carboxamidotryptamine binding sites with a similar Kd value. However, Bmax in the CM was 4-times higher than that in the LM.The molecular study (reverse transcription polymerase chain reaction) demonstrated that the expression of 5-HT_7 receptor mRNA in the CM was higher than that in the LM.5-HT increased the cAMP in both muscle layers, but the increment in the CM was higher than that in the LM.Therefore, it was suggested that the different inhibition of the contractility by 5-HT was caused by muscle layer-related accumulation of cAMP (CM>LM), due to muscle-layer dependent distribution (CM>LM) of 5-HT_7 receptors. This heterogeneous distribution of 5-HT_7 receptor is quite unique and different from that of other receptors (muscarinc M_3 receptor, histamine H_1 receptor , endothelin A receptor, β and α2-adrenaline receptors, LM>CM) present in the porcine uterus. Muscle layer-dependent inhibition : 5-HT_7 and β_2-adrenaline receptor-mediatd pathways were the main inhibitory mechanisms in the porcine myometrium and shared in the inhibition of contractility in a smooth muscle layer-dependent manner (LM : β_2-adrenoceptors, CM : 5-HT_7 receptors). The responses of 5-HT and isoproterenol differ from region to region, probably because of differences in the dominant subtype of 5-HT receptors and density of β_2-adrenaline receptors. Bioactive peptides and nitric oxide do not play a significant physiological role (as inhibitory agents) in the regulation of porcine uterine contractility. |
| 日本学術振興会, 科学研究費助成事業, ブタの子宮縦走筋ならびに輸走筋におけるの2受容体性収縮機構の解明, 1993, 1993, 種池 哲朗; 寺岡 宏樹; 北澤 多喜雄, 1.収縮実験:【.encircled1.】ノルエピネフリン(NE,10nM-10muM,propranolol処置)は、Krebs液(Ca^<2+>2.5mM;37°C)液下で発情前期のブタ子宮角縦走筋(LM)標本を濃度依存性に収縮させた。この収縮は、TIX耐性で、alpha遮断薬phentolamine,alpha2遮断薬yohimbine(Yoh.10nM-1muM,pA2=8.37±0.06)により競合的に抑制されたが、alpha1遮断薬prazosin(Pra.1muM)では抑制されなかった。alpha2作動薬clonidine>xylazaine>>alpha1作動薬phenylephrine≧methoxamineの順番でLMを収縮させた。TMSで得られるLMの収縮は、TTX,phenotolamine,Yoh.(10-100nM)で抑制されたが、Pra.(1muM)では抑制されなかった。輪走筋(CM)はalpha1-とalpha2-作動薬に感受性を示さなかった。【.encircled2.】Ebashi-Kumagai液(Ca^<2+>0.4mM・Mg^<2+>0.2mM;28°C)下の収縮力価は、NE>phenylephrine>>methoxamineで、clonidine,xylazaineは100muMでも効果がなかった。NE収縮は、Yoh.(pA2=8.38±0.13)により競合的に抑制されたが、Pra.では非競合的に抑制された。受容体結合実験:粗製膜標本と放射性alpha1受容体リガンド^3H-prazosin、alpha2受容体^3H-rauwolscineとの結合が飽和性で特異的であった。^3H-prazosinの解離定数(kd値、nmol/1,n=4)と最大結合数(Bmax値、fmol/mg protein)は、LMで0.35±0.037,35.86±10.13,CMでは0.28±0.008,9.68±5.37であった。^3H-rauwolscineのKdとBmaxは、LMで3.09±0.26,255.61±25.15,CMでは2.86±0.19,62.64±6.26であった。以上より、ブタ子宮筋に存在するシナプス後受容体は、主にalpha2タイプであり、CMよりもLMに多く外因性および内因性NEの収縮反応の発現に関与していることを示している。しかし、このalpha2受容体機構は、低Ca^<2+>、低Mg^<2+>、低温下では受容体あるいはそれ以降のレベルで作動しずらくなるものと考えられる。 2.TMSの刺激頻度依存性にNE放出量は増加した。この放出増加はCa^2-free液中またはTTX処置で抑制された。誘発性のNE放出は、Yoh.,idazoxane,rauwolscineによりそれぞれ著明に増大したが、Pra.,clonidine,xylazaine,epinephrineによっては影響を受けなかった。これらより、シナプス前alpha2受容体を介するシナプス前抑制機構が作動しているか否かについては更なる検討が必要である。 3.静止時の子宮縦走筋のcAMP含量は約4 pmol/mg proteinで、IBMX(1mM)により増加する傾向が見られた。Isoproterenolは濃度依存性(0.1-100nM)にcAMP合成を刺激したが、収縮の最大反応を起こす1muMclonidineはIBMXの存在に関わらずcAMP含量に全く影響を与えなかった。従って、alpha2受容体刺激によるブタ子宮縦走筋の収縮反応にアデニレートシクラーゼ系は関与していないものと考えられた。 |
| 日本学術振興会, 科学研究費助成事業, Substance Pアミノ酸構造の系統発生過程での変化に関する基礎的研究, 1989, 1989, 北澤多喜雄 |
| 日本学術振興会, 科学研究費助成事業, コイ腸管膨大部におけるsubstance pの神経伝達物質としての可能性について, 1987, 1987, 北澤多喜雄 |
| 日本学術振興会, 科学研究費助成事業, コイ腸管膨大部の非Choline作動性収縮に関与する生理活性peptideの検索, 1986, 1986, 北澤多喜雄 |
| 日本学術振興会, 科学研究費助成事業, 消化管平滑筋神経支配様式の系統発生過程での変化に関する基礎的研究, 1984, 1984, 北澤多喜雄 |
| 日本学術振興会, 科学研究費助成事業, アドレナリン作動性刺激が消化管平滑筋に及ぼす影響に関する系統発生学的研究, 1983, 1983, 北澤 多喜雄 |
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