School of Veterinary Medicine, Department of Veterinary Medicine
翁長武紀 オナガ タケノリ
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Name
ONAGA Takenori
Affiliation (Master)
School of Veterinary Medicine, Department of Veterinary Medicine
Affiliation & Job
Rakuno Gakuen University School of Veterinary Medicine Department of Veterinary Medicine
Education
- 1990 , Rakuno Gakuen University, Faculty of Dairy Science - 1990 , Rakuno Gakuen University, Faculty of Dairy Science
Degree
Ph. D.(Veterinary Medicine), Hokkaido University
Association Memberships
日本生理学会 日本畜産学会 日本獣医学会 Physiological Society of Japan Japanese Society of Animal Science Japanese Society of Veterinary Science
Committee Memberships
2008 , Japanese Society of Veterinary Science, trustee
Research Activities
Research Areas
Life sciences, Veterinary medicine
Research Interests
膵外分泌, 反芻動物, 生理活性ペプチド, 比較内分泌, 消化管運動, Pancreas, Small Intestine, Abomasum, Rumen, Ruminants, EGF, NO, PYY, PACAP, Parasympathetic Nerve, Enteric Nervous System, Comparative Endocrinology, Gastrointestinal Motility,
Published Papers
Transgenic Zebrafish Expressing Rat Cytochrome P450 2E1 (CYP2E1): Augmentation of Acetaminophen-Induced Toxicity in the Liver and Retina, Yoshinori Sato; Wenjing Dong; Tatsuro Nakamura; Naohiro Mizoguchi; Tasuku Nawaji; Miyu Nishikawa; Takenori Onaga; Shinichi Ikushiro; Makoto Kobayashi; Hiroki Teraoka, International Journal of Molecular Sciences, MDPI AG, 24, (4) 4013 - 4013, 2023 02 16 Intravenous administration of xenin-25 accelerates cyclic ruminal contractions in healthy conscious sheep., Takenori Onaga; Ami Sakai; Yumiko Yasui, Neuropeptides, 96, 102293 - 102293, 2022 12 Neurotensin and xenin stimulates pancreatic exocrine secretion through the peripheral cholinergic nerves in conscious sheep., Takenori Onaga; Yumiko Yasui; Hideaki Hayashi, General and comparative endocrinology, 326, 114073 - 114073, 2022 09 15 Effects of xenin-25 on insulin and glucagon secretions in healthy conscious sheep., Takenori Onaga; Hideaki Hayashi; Yumiko Yasui, Domestic animal endocrinology, 77, 106635 - 106635, 2021 10 Messenger RNA expression and localization of xenin in the gastrointestinal tract in sheep, T. Onaga; A. Sakai; M. Kajita; H. Fukuda; Y. Yasui; H. Hayashi, Domestic Animal Endocrinology, Elsevier BV, 74, 106523 - 106523, 2021 01 Role of neurotensin in the regulation of gastric motility in healthy conscious sheep, Takenori Onaga; Tomohiko Shimoda; Taro Ohishi; Yumiko Yasui; Hideaki Hayashi, Small Ruminant Research, Elsevier BV, 172, 31 - 41, 2019 03 An Examination of Parasitic Infection and Cortisol Measurement with Feces from Captive Apes Kept in a Zoological Garden, AKIBA Yuki; KOIDE Kentaro; HIRATA Haruyuki; ONAGA Takenori; IWANO Hidetomo; YOKOTA Hiroshi; ASAKAWA Mitsuhiko; YOSHIDA Junichi; TAKAESU Noboru; ISHIBASHI Hiroki; WATANABE Yoko; TAKEDA Masahiro; ISHII Kazuko; IWATA Koichi; YAMAMOTO Tatsuya, Japanese Journal of Zoo and Wildlife Medicine, Japanese Society of Zoo and Wildlife Medicine, 23, (2) 27 - 31, 2018 Characterization of feline cytochrome P450 2B6, Gaku Okamatsu; Tetsuya Komatsu; Yuka Ono; Hiroki Inoue; Tsuyoshi Uchide; Takenori Onaga; Daiji Endoh; Takio Kitazawa; Takeo Hiraga; Yasuhiro Uno; Hiroki Teraoka, XENOBIOTICA, 47, (2) 93 - 102, 2017 Identification and functional characterization of novel feline cytochrome P450 2A, Gaku Okamatsu; Tetsuya Komatsu; Akira Kubota; Takenori Onaga; Tsuyoshi Uchide; Daiji Endo; Rikio Kirisawa; Guojun Yin; Hiroki Inoue; Takio Kitazawa; Yasuhiro Uno; Hiroki Teraoka, XENOBIOTICA, 45, (6) 503 - 510, 2015 06 Assessment of Testicular Corticosterone Biosynthesis in Adult Male Rats, Naoyuki Maeda; Sachi Tahata; Takeshi Yagi; Emi Tanaka; Kanae Masu; Michiko Sato; Satoko Haeno; Takenori Onaga; Hiroshi Yokota, PLOS ONE, 10, (2) , 2015 02 Tachykinin: Recent developments and novel roles in health and disease, Takenori Onaga, Biomolecular Concepts, De Gruyter Mouton, 5, (3) 225 - 243, 2014 06 01 Polymeric immunoglobulin receptor expression and local immunoglobulin A production in bovine sublingual, submandibular and parotid salivary glands, K. Sakaguchi; H. Yokota; T. Miyasho; N. Maeda; K. Nakamura; T. Onaga; M. Koiwa; K. Matsuda; M. Okamoto; K. Hirayama; H. Taniyama, Veterinary Journal, 197, (2) 291 - 296, 2013 08 Role of tachykinins and neurokinin receptor subtypes in the regulation of motility of the forestomach and abomasum in conscious sheep, Taro Oh-ishi; Tomohiko Shimoda; Hideaki Hayashi; Takenori Onaga, NEUROPEPTIDES, 47, (1) 9 - 18, 2013 02 Fatty acid-binding protein expression in the gastrointestinal tract of calves and cows, Hideaki Hayashi; Sachiko Maruyama; Madoka Fukuoka; Takaharu Kozakai; Keiichi Nakajima; Takenori Onaga; Seiyu Kato, ANIMAL SCIENCE JOURNAL, 84, (1) 35 - 41, 2013 A Case of Preliminary Utilization of Commercial ELISA Kit for Detecting Excretion of Urinary 8-hydroxyguanosine (8-OHdG) in Captive Chimpanzees, Pan troglodytes, Ohshima, Yuko; Ai Mizuo; Keiko Sugama; Genichi Idani; Ryoji Shimura; Akiko Uebayachi; Satoru Takahashi; Manabu Onuma; Takenori Onaga; Katsuro Hagiwara; Mutsuhiko Asakawa, Journal of Jpn. Assoc. Zoo Aqu., 日本動物園水族館協会, 52, (4) 140 - 144, 2012 04 Role of tachykinin and neurokinin receptors in the regulation of ovine omasal contractions, Takenori Onaga; Taro Oh-ishi; Tomohiko Shimoda; Seira Nishimoto; Hideaki Hayashi, REGULATORY PEPTIDES, 173, (1-3) 64 - 73, 2012 01 ラット小腸寄生線虫Orientostrongylus ezoensisの感染時における胃の影響, 山田 清太郎; 大西 広輔; 田澤 寿保; 石原 智明; 植田 弘美; 翁長 武紀; 北澤 多喜雄; 平田 晴之; 福本 真一郎, 獣医寄生虫学会誌, 日本獣医寄生虫学会, 9, (2) 134 - 134, 2011 03 ラット小腸寄生線虫Orientostrongylus ezoensisの感染時における胃の影響, 山田 清太郎; 田澤 寿保; 石原 智明; 植田 弘美; 翁長 武紀; 北澤 多喜雄; 平田 晴之; 福本 真一郎, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 150回, 183 - 183, 2010 09 Ghrelin stimulates gastric motility of the guinea pig through activation of a capsaicin-sensitive neural pathway: in vivo and in vitro functional studies, T. Nakamura; T. Onaga; T. Kitazawa, NEUROGASTROENTEROLOGY AND MOTILITY, 22, (4) 446 - +, 2010 04 Role of nitrergic nerves in the regulation of motility of the omasum and abomasum in healthy sheep (Ovis aries), Takenori Onaga; Noriko Hara; Yasuhito Shimizu, VETERINARY RESEARCH COMMUNICATIONS, 33, (1) 33 - 48, 2009 01 Preliminary Research on the Excretion of Urinary 8-Hydroxyguanosine (8-OHdG) as a Marker of Protozoan Parasites Infection in Captive Western Lowland Gorillas (Gorilla gorilla gorilla), MIZUO Ai; ONUMA Manabu; ONAGA Takenori; HAGIWARA Katsuro; SANADA Yoshinori; ASAKAWA Mitsuhiko; OHSHIMA Yuko; IMANISHI Ryo; KITADA Yuji; KASAHARA Michiko; HASHIZAKI Fumitaka; WADA Seitaro; MATUNAGA Masayuki; TAKAI Susumu, Japanese Journal of Zoo and Wildlife Medicine, Japanese Society of Zoo and Wildlife Medicine, 14, (1) 77 - 80, 2009 Localization of CCK-1 R in the omasum and role of CCK in the regulation of ornasal contractions in sheep, Takenori Onaga; Aya Sugita; Rena Wakaiki; Ichiro Hara; Kanji Kagawa; Rikio Kirisawa; Hideaki Hayashi, DOMESTIC ANIMAL ENDOCRINOLOGY, 35, (2) 231 - 244, 2008 08 Effect of intravenous infusion of proglumide on ruminal motility in conscious sheep (Ovis aries), T. Onaga, VETERINARY RESEARCH COMMUNICATIONS, 31, (8) 1021 - 1036, 2007 11 Localization and secretion of epidermal growth factor in the parotid gland and its intragastric kinetics in sheep, Takenori Onaga; Yasuhito Shimizu; Hideaki Hayashi; Masayoshi Tsuji; Daiji Endoh; Hiroyuki Okada, LIFE SCIENCES, 79, (17) 1616 - 1629, 2006 09 The Molecular Marker NTx Reflecting Changes in Tissue Remodeling Associated with Injury to the Equine Superficial Digital Flexor Tendon, HOSAKA Yoshinao; HIGUCHI Hidetoshi; ONAGA Takenori; NAGATA Hiroshi; KAMIKURA Tomoki; MISUMI Kazuhiro; UEDA Hiromi; Kazushige, Journal of the Japan Veterinary Medical Association, Japan Veterinary Medical Association, 58巻,第3号,p.175-179, (3) 175 - 179, 2005 Multiple regulation of peptide YY secretion in the digestive tract, T Onaga; R Zabielski; S Kato, PEPTIDES, 23, (2) 279 - 290, 2002 02 Effects of nitric oxide donor and nitric oxide synthase inhibitor on ruminal contractions in conscious sheep, T Onaga; H Okada; S Hagiwara; C Nagashima; H Inoue; W Korczynski; S Kato, RESEARCH IN VETERINARY SCIENCE, 71, (3) 189 - 195, 2001 12 Role of peptide tyrosine tyrosine (PYY) in the regulation of gastrointestinal motility and pancreatic exocrine secretion in sheep, Takenori Onaga, Hokkaido University, Hokkaido University, 49, (1) 30 - 31, 2001 Effect of nitric oxide synthase inhibitors on the temporal coordination of duodenal contractions and pancreatic exocrine secretion in sheep, T Onaga; C Nagashima; T Sakata, JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY, 170, (5-6) 469 - 479, 2000 09 Regional distribution and plasma concentration of peptide YY in sheep, T Onaga; M Yoshida; H Inoue; H Yokota, PEPTIDES, 21, (5) 655 - 667, 2000 05 Small intestinal UDP-glucuronosyltransferase sheUGT1A07: Partial purification and cDNA cloning from sheep small intestine, T Kobayashi; A Tatano; H Yokota; T Onaga; T Watanabe; A Yuasa, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 364, (2) 143 - 152, 1999 04 Pituitary adenylate cyclase-activating polypeptide (PACAP) induces duodenal phasic contractions via the vagal cholinergic nerves in sheep, T Onaga; Y Harada; K Okamoto, REGULATORY PEPTIDES, 77, (1-3) 69 - 76, 1998 10 Effects of proglumide on cholecystokinin-8-induced exocrine and endocrine pancreatic responses in conscious sheep, H. Mineo; N. Iwaki; K. Kogishi; T. Onaga; S. Kato; R. Zabielski, Comparative Biochemistry and Physiology - A Physiology, 118, (3) 759 - 764, 1997 11 PACAP stimulates pancreatic exocrine secretion via the vagal cholinergic nerves in sheep, T Onaga; K Okamoto; Y Harada; H Mineo; S Kato, REGULATORY PEPTIDES, 72, (2-3) 147 - 153, 1997 10 PGE(2) inhibits glucose uptake in isolated villous epithelial cells of the ovine small intestine, HS Hyun; S Arai; T Onaga; S Kato, JOURNAL OF VETERINARY MEDICINE SERIES A-PHYSIOLOGY PATHOLOGY CLINICAL MEDICINE, 44, (7) 443 - 447, 1997 09 Role of peptide YY in regulation of duodenal motility during the interdigestive period in sheep, T Onaga; S Kubagawa; S Lee; H Mineo; S Kato; S Naruse; T Ozaki, JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY, 167, (5) 352 - 360, 1997 07 Effects of oxytocin, arginine-vasopressin and lysine-vasopressin on insulin and glucagon secretion in sheep, H Mineo; M Ito; H Muto; H Kamita; HS Hyun; T Onaga, RESEARCH IN VETERINARY SCIENCE, 62, (2) 105 - 110, 1997 03 Responses of glucose absorption and fructose absorption to prostaglandin E2 in intestinal loops of sheep, HS Hyun; T Onaga; H Mineo; S Kato, RESEARCH IN VETERINARY SCIENCE, 62, (2) 153 - 157, 1997 03 Effects of C-terminal fragments of cholecystokinin on plasma insulin and glucagon concentrations in sheep, H Mineo; H Kamita; H Muto; M Ito; HS Hyun; T Onaga, RESEARCH IN VETERINARY SCIENCE, 62, (2) 171 - 174, 1997 03 PACAP contracts the ovine duodenum via the vagal cholinergic nerves, T Onaga; Y Harada; K Okamoto, ADVANCES IN COMPARATIVE ENDOCRINOLOGY, TOMES 1 AND 2, 693 - 696, 1997 Effect of L364 718 on interdigestive pancreatic exocrine secretion and gastroduodenal motility in conscious sheep, T Onaga; H Mineo; S Kato, REGULATORY PEPTIDES, 68, (2) 139 - 146, 1997 01 Effect of cholera toxin on glucose absorption and net movements of water and electrolytes in the intestinal loop of sheep, HS Hyun; T Onaga; H Mineo; JT Kim; S Kato, JOURNAL OF VETERINARY MEDICINE SERIES A-PHYSIOLOGY PATHOLOGY CLINICAL MEDICINE, 43, (10) 611 - 618, 1996 12 Comparison of the effects of secretagogues on the net movement of electrolyte and glucose absorption in the proximal and middle small intestine of sheep, HS Hyun; T Onaga; H Mineo; S Kato, JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION, 76, (4-5) 160 - 169, 1996 11 Effect of pituitary adenylate cyclase-activating polypeptide on exocrine and endocrine secretion in the ovine pancreas, T Onaga; M Uchida; M Kimura; M Miyazaki; H Mineo; S Kato; R Zabielski, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY, 115, (3) 185 - 193, 1996 11 Loperamide does not affect the prostaglandin E(2) (PGE(2))-induced anti-absorptive effect on glucose in the small intestinal loop of sheep, HS Hyun; T Onaga; H Mineo; S Kato, JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION-ZEITSCHRIFT FUR TIERPHYSIOLOGIE TIERERNAHRUNG UND FUTTERMITTELKUNDE, 76, (2-3) 80 - 89, 1996 09 Effects of postoperative periods on glucose absorption and electrolyte secretion in the intestinal loop of sheep, HS Hyun; T Onaga; H Mineo; S Kato, JOURNAL OF VETERINARY MEDICINE SERIES A-ZENTRALBLATT FUR VETERINARMEDIZIN REIHE A-PHYSIOLOGY PATHOLOGY CLINICAL MEDICINE, 42, (9) 601 - 610, 1995 11 The effect of PGE(2) on glucose absorption and net movement of water and electrolytes in the jejunal loop in sheep, HS Hyun; T Onaga; H Mineo; S Kato, JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION-ZEITSCHRIFT FUR TIERPHYSIOLOGIE TIERERNAHRUNG UND FUTTERMITTELKUNDE, 74, (4-5) 185 - 193, 1995 11 Effect of neural blockades, gastrointestinal regulatory peptides, and diversion of gastroduodenal contents on periodic pancreatic secretion in the preruminant calf, R Zabielski; P Kiela; T Onaga; H Mineo; PC Gregory; S Kato, CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 73, (11) 1616 - 1624, 1995 11 EFFECT OF VASOACTIVE INTESTINAL POLYPEPTIDE (VIP) ON THE NET MOVEMENT OF ELECTROLYTES AND WATER AND GLUCOSE-ABSORPTION IN THE JEJUNAL LOOP OF SHEEP, HS HYUN; T ONAGA; H MINEO; S KATO, JOURNAL OF VETERINARY MEDICAL SCIENCE, 57, (5) 865 - 869, 1995 10 ROLE OF ENDOGENOUS CCK IN REGULATION OF INTERDIGESTIVE PANCREATIC EXOCRINE SECRETION IN SHEEP (OVIS-ARIES), S TACHIBANA; T ONAGA; H MINEO; S KATO, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY, 112, (1) 103 - 109, 1995 09 EFFECTS OF BENZOIC-ACID AND ITS ANALOGS ON INSULIN AND GLUCAGON-SECRETION IN SHEEP, H MINEO; T OHDATE; K FUKUMURA; T KATAYAMA; T ONAGA; S KATO; N YANAIHARA, EUROPEAN JOURNAL OF PHARMACOLOGY, 280, (2) 149 - 154, 1995 07 INTRADUODENAL CHOLECYSTOKININ-OCTAPEPTIDE (CCK-8) CAN STIMULATE PANCREATIC-SECRETION IN THE CALF, R ZABIELSKI; T ONAGA; H MINEO; S KATO; SG PIERZYNOWSKI, INTERNATIONAL JOURNAL OF PANCREATOLOGY, 17, (3) 271 - 278, 1995 06 EFFECTS OF INTRAVENOUS INFUSIONS OF CHOLECYSTOKININ (CCK)-8 ON EXOCRINE AND ENDOCRINE PANCREATIC-SECRETION IN CONSCIOUS SHEEP, H MINEO; N IWAKI; K KOGISHI; R ZABIELSKI; T ONAGA; S KATO, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY, 111, (1) 133 - 138, 1995 05 CHOLECYSTOKININ DOES NOT ACT ON THE EFFERENT PATHWAY OF CHOLINERGIC AND ADRENERGIC-NERVES TO INHIBIT RUMINAL CONTRACTIONS IN SHEEP (OVIS-ARIES), T ONAGA; T ONODERA; H MINEO; S KATO, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY, 111, (1) 51 - 58, 1995 05 Effect of intravenous infusions of cholecystokinin-8 and pentagastrin on plasma concentration of insulin and glucagon in sheep, Mineo; H. Iwaki; N. Onaga; T. Kato, Research in Veterinary Science, 56, (3) 298 - 302, 1995 PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE (PACAP) STIMULATES EXOCRINE PANCREAS IN CONSCIOUS PRERUMINATING CALVES, R ZABIELSKI; T ONAGA; H MINEO; E OKINE; S KATO, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 109, (1) 93 - 99, 1994 09 CHEMICAL SPECIFICITY OF SHORT-CHAIN FATTY-ACIDS IN STIMULATING INSULIN AND GLUCAGON-SECRETION IN SHEEP, H MINEO; Y HASHIZUME; Y HANAKI; K MURATA; H MAEDA; T ONAGA; S KATO; N YANAIHARA, AMERICAN JOURNAL OF PHYSIOLOGY, 267, (2) E234 - E241, 1994 08 EFFECTS OF PERFUSION RATE AND GLUCOSE-CONCENTRATION ON GLUCOSE-ABSORPTION IN INTESTINAL THIRY-VELLA LOOP IN SHEEP, HS HYUN; T ONAGA; H MINEO; S KATO, JOURNAL OF VETERINARY MEDICAL SCIENCE, 56, (3) 617 - 618, 1994 06 EFFECTS OF INTRAVENOUS INFUSIONS OF CHOLECYSTOKININ-8 AND PENTAGASTRIN ON PLASMA-CONCENTRATIONS OF INSULIN AND GLUCAGON IN SHEEP, H MINEO; N IWAKI; T ONAGA; S KATO, RESEARCH IN VETERINARY SCIENCE, 56, (3) 298 - 302, 1994 05 PLASMA SECRETIN FLUCTUATES IN-PHASE WITH PERIODIC PANCREATIC-SECRETION AND THE DUODENAL MIGRATING MYOELECTRIC COMPLEX IN CALVES, R ZABIELSKI; Y TERUI; T ONAGA; H MINEO; S KATO, RESEARCH IN VETERINARY SCIENCE, 56, (3) 332 - 337, 1994 05 LOCAL VERSUS PERIPHERAL-BLOOD ADMINISTRATION OF CHOLECYSTOKININ-8 AND SECRETIN ON PANCREATIC-SECRETION IN CALVES, R ZABIELSKI; T ONAGA; H MINEO; SG PIERZYNOWSKI; S KATO, EXPERIMENTAL PHYSIOLOGY, 79, (3) 301 - 311, 1994 05 Chemical specificity of short-chain fatty acids in stimulating insulin and glucagon secretion in sheep, H. Mineo; Y. Hashizume; Y. Hanaki; K. Murata; H. Maeda; T. Onaga; S. Kato; N. Yanaihara, American Journal of Physiology - Endocrinology and Metabolism, 267, (2) E234 - E241, 1994 Pituitary adenylate cyclase-activating polypeptide (PACAP) stimulates exocrine pancreas in conscious preruminating calves, R. Zabielski; T. Onaga; H. Mineo; E. Okine; S. Kato, Comparative Biochemistry and Physiology. Part C: Comparative, 109, (1) 93 - 99, 1994 EXOCRINE PANCREATIC RESPONSES TO CCK-8 AND VIP INFUSED DURING NSA AND RSA PHASE OF DUODENAL MMC IN CALVES - DOES PANCREATIC RESPONSIVENESS TO GI PEPTIDES FLUCTUATE PERIODICALLY, R ZABIELSKI; T ONAGA; H MINEO; S KATO, BIOMEDICAL RESEARCH-TOKYO, 15, 371 - 375, 1994 THE INHIBITION OF INTERDIGESTIVE PERIODIC PANCREATIC-SECRETION BY PYY IN DOGS, T ONAGA; R ZABIELSKI; S KATO; S NARUSE; T OZAKI, BIOMEDICAL RESEARCH-TOKYO, 15, 377 - 381, 1994 PERIODIC FLUCTUATIONS IN PANCREATIC-SECRETION AND DUODENAL MOTILITY INVESTIGATED IN NEONATAL CALVES, R ZABIELSKI; T ONAGA; H MINEO; S KATO, EXPERIMENTAL PHYSIOLOGY, 78, (5) 675 - 684, 1993 09 若齢犬における損傷坐骨神経の再生(共著), 日本獣医学会雑誌, 45-46, 775 - 779, 1993 EFFECTS OF PERIPHERAL AND LOCAL VIP ADMINISTRATION ON SECRETION OF EXOCRINE PANCREAS AND ON ELECTRICAL AND MECHANICAL-ACTIVITY OF DUODENUM IN PRERUMINATING CALVES, R ZABIELSKI; T ONAGA; H MINEO; S KATO, BIOMEDICAL RESEARCH-TOKYO, 13, 243 - 246, 1992 Effect of intravenous infusion of insulin, glucose and tolbutamide on pancreatic exocrine secretion in sheep, Kato, S; S. Matsumoto; H. Kojima; H. Mineo; T. Onaga; K. Kikuchi, Japanese Journal of Zootechnology and Sciense, Japanese Society of Animal Science, 61, (4) 360 - 365, 1990 Effect of cholecystokinin octapeptide on contraction of bovine ruminal muscle, Onaga, T; S. Kato; H. Mineo; J. Ushijima, Asian-Australasian Journal of Animal Science, 2, 165 - 166, 1989
Books etc
Duke's Physiology of Domestic Animals, 監訳; 鈴木, 浩悦; 澁谷, 泉; 翁長, 武紀; 田谷, 一善, 42～46章, 学窓社, 2020 03 , 9784873627724 これからの乳牛群管理のためのハードヘルス学<成牛編>, 翁長 武紀, 3-1 反芻動物の栄養生理, 緑書房, 2017 12 実験で学ぶ生理学 [改訂版], 翁長 武紀, 消化吸収、腎臓、消化管平滑筋, 学窓社, 2014 03 実験で学ぶ生理学, 翁長 武紀, 消化吸収、腎臓、消化管平滑筋, 学窓社, 2009 10
Misc
Ghrelin Stimulates Gastric Motility Through Activation of the Vago-Vagal Reflex Pathway in the Anesthetized Guinea-Pig, Takio Kitazawa; Takenori Onaga; Tatsuro Nakamura, GASTROENTEROLOGY, 136, (5) A534 - A534, 2009 05 Ghrelin stimulates gastric motility of the anesthetized guinea-pig, Nakamura Tatsuro; Onaga Takenori; Kitazawa Takio, Journal of pharmacological sciences, 109, (1) 181 - 181, 2009 03 Ghrelin stimulates gastric motility of the anesthetized guinea-pig, Tatsuro Nakamura; Takenori Onaga; Takio Kitazawa, JOURNAL OF PHARMACOLOGICAL SCIENCES, 109, 2009 ROLE OF VIP IN THE REGULATION OF OMASAL MOTILITY IN SHEEP, Takenori Onaga; Yuji Takayama; Ei-ichi Tsuyama; Kanji Kagawa, JOURNAL OF PHYSIOLOGICAL SCIENCES, 59, 2009 ヒツジにおける延髄のPACAP結合部位と上部消化管機能の調節, 翁長武紀; 石橋寛子; 遠藤大二; 保坂善真; 竹花一成, 日本獣医学会学術集会講演要旨集, 139th, 2005
Research Grants & Projects
日本学術振興会, 科学研究費助成事業, 内分泌細胞に富む消化管オルガノイドの開発と消化管ホルモンの分泌調節因子の解明, 20210401, 20240331, 翁長 武紀, オルガノイドの開発において、Xenin内分泌細胞は生体内では胃および十二指腸粘膜に高密度に局在することから、先ずラットの胃粘膜をカルシウムキレート法で処理して陰窩を含む上皮細胞を分離してマトリゲル内で3次元培養した。これにWnt,、Noggin、R-spomdin、上皮成長因子EGFや線維芽細胞成長因子FGF、ガストリンなどの各種分化促進因子を適用し、初期培養液中に必要なニコチンアミドやRHOK-inhibitorであるY-27632をある時期以降から減らすとオルガノイド形成が進み、内分泌細胞への分化も増えると報告されているが、実際行ったところその通りの結果が得られていない。1回目は約4週間、2回目は約6週間培養しているが、細胞は継代できても、陰窩の幹細胞が分化して、球形のオルガノイドを形成するに至っていない。動物種は異なるが、既報に従った組成の培養液を調製しているが、ラットの胃上皮培養細胞の分化が進まず、初年度の目標であった転写因子を遺伝子導入して内分泌細胞に富むオルガノイドを作製するところまで進んでいない。
培養細胞の分化の進行を確認するための複数のPCR系は別途準備できているが、培養細胞の形態的な分化が不十分であるので、分化を検証するに至っていない。
調節遺伝子のクローニングについては、オルガノイドにノックインするラットの転写因子のうち、十二指腸への分化に関わるラットPdx1はクローニングできたが、胃の分化に関わるラットのBapx1のクローニングが一部未完成である。 Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Ghrelin peptides induced contractions and growth hormone secretagogue receptor expression in the chicken gastrointestinal tract, 2004, 2006, KITAZAWA Takio; ONAGA Takenori; IWANO Hidetomc, Background and aim. Ghrelin is the endogenous ligand for the growth hormone secretagogue receptor (GHS-R), and stimulates food intake and gastrointestinal (GI) motility in mammals. In chickens, on the other hand, ghrelin inhibits food intake. This discrepancy prompted us to investigate whether ghrelin affects gastrointestinal motility in the chicken. Methods, The contractile effect of chicken ghrelin was measured isometrically in muscle strips from different regions of the GI tract suspended in an organ bath. The effect of chicken ghrelin was compared with that of rat or human ghrelin. In addition, mRNA expression of ghrelin and GHS-R was examined by quantitative RT-PCR.
Results. Among the ghrelin peptides tested (rat, human and chicken ghrelin), only chicken ghrelin caused contraction of muscle strips from the GI tract. No effect was seen in des-octanoylated chicken ghrelin, suggesting that octanoylation at Ser^3 residue was essential for its activity. The contractile response to chicken ghrelin in the crop was not affected by tetrodotoxin (TTX), but that in the proventriculus was decreased by TTX and atropine to the same extent. D-Lys^3-GHRP-6 (a GHS-R antagonist) caused a transient contraction and inhibited the chicken ghrelin-induced contraction without affecting the 50 mM high-K^+-induced contraction. The amplitude of the chicken ghrelin-induced contraction was different among GI regions: highest in the crop and colon (70% of high-K^+-induced contraction), moderate in the esophagus and proventriculus (30%), and weak in the small intestine (10-20%) and the ranking order was consistent with GHS-R mRNA expression in each region (colon > crop = esophagus > proventriculus > duodenum =jejunum > ileum). GHS-R mRNA expression in the muscle preparations of crop and proventriculus without mucosa was 2-4 times higher than that in whole segments of crop and proventriculus, indicating that the GHS-R is mainly expressed in smooth muscle layers including enteric nerves. In contrast to the GHS-R expression, ghrelin mRNA was mainly expressed in the mucosa of proventriculus and duodenum.
Conclusion. These results indicate that ghrelin stimulates GI contractility and preferentially affects the motor activity of esophagus, crop and colon through myogenic and/or neurogenic mechanisms. These actions are mediated through the GHS-R, which is highly sensitive to chicken ghrelin 日本学術振興会, 科学研究費助成事業, 反芻動物における上皮増殖因子の分泌と胃内動態の研究, 2001, 2002, 翁長 武紀, 昨年度はヒツジのEGF量を測定するラジオレセプターアッセイ(RRA)を確立して、ヒツジの唾液腺におけるEGFの分布と組織含量を調べ、およびEGF活性物質の部分精製を行ったが、今年度はEGFの唾液中の分泌動態および胃内動態を検討した。方法として、手術によりヒツジの耳下腺管に挿管した後、無麻酔下において耳下腺から分泌された唾液を採取してRRAにより分泌動態を調べ、集めた唾液を低圧液体クロマトグラフィーおよびHPLCにより分析して下記の結果を得た。1)唾液腺から分離した高分子型のEGF活性物質は耳下腺の唾液中には検出されず、分子量およそ6kDaの低分子型EGFが検出された。2)食間期(10:00-18:00)の耳下腺唾液におけるEGFの濃度は582.0±33.26pM、分泌量は0.40±0.03pmol/minであり、摂食時(18:00-19:00)に濃度が1015.5±102.47pM、分泌量が2.30±0.40pmol/minに増加した。さらに胃に挿管して胃液を採取し、そのEGF濃度を調べたところ、3)第一胃液中のEGF濃度は食間期で73.3±6.0pMであり、食後に339.1±125.4pMに増加した。4)第四胃液中のEGF濃度は食間期で659.2±120.0pMであり、食後に259.4±157.3nMにまで著しく増加した。以上の結果より、唾液腺組織中の高分子型のEGF活性物質はおそらくEGFの前駆物質であり、ヒツジでは食間期においても唾液中に低分子量の分泌型EGFが常に分泌されていることが明らかとなった。その分泌は食後に著しく増加し、それに一致して第一胃内のEGF濃度も増加することが明らかとなった。また、第四胃液内のEGF濃度も食後に増加するが、第一胃よりも濃度上昇が著しいことから、唾液腺以外からの分泌によって第四胃内のEGF濃度が上昇することが示唆された。 反芻動物の消化管運動の調節機序に関する研究, 1992 消化管機能のペプチド作動性調節機序に関する研究, 1992 Study on the regulatory mechanism of gastrointestinal motility in ruminants, 1992 Study on peptidergic regulations of gastrointestinal functions, 1992
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Researcher Profile and Settings
Name
| ONAGA Takenori |
Affiliation (Master)
| School of Veterinary Medicine, Department of Veterinary Medicine |
Affiliation & Job
| Rakuno Gakuen University |
| School of Veterinary Medicine Department of Veterinary Medicine |
Education
| - 1990 , Rakuno Gakuen University, Faculty of Dairy Science |
| - 1990 , Rakuno Gakuen University, Faculty of Dairy Science |
Degree
| Ph. D.(Veterinary Medicine), Hokkaido University |
Association Memberships
| 日本生理学会 |
| 日本畜産学会 |
| 日本獣医学会 |
| Physiological Society of Japan |
| Japanese Society of Animal Science |
| Japanese Society of Veterinary Science |
Committee Memberships
| 2008 , Japanese Society of Veterinary Science, trustee |
Research Activities
Research Areas
| Life sciences, Veterinary medicine |
Research Interests
| 膵外分泌, |
| 反芻動物, |
| 生理活性ペプチド, |
| 比較内分泌, |
| 消化管運動, |
| Pancreas, |
| Small Intestine, |
| Abomasum, |
| Rumen, |
| Ruminants, |
| EGF, |
| NO, |
| PYY, |
| PACAP, |
| Parasympathetic Nerve, |
| Enteric Nervous System, |
| Comparative Endocrinology, |
| Gastrointestinal Motility, |
Published Papers
| Transgenic Zebrafish Expressing Rat Cytochrome P450 2E1 (CYP2E1): Augmentation of Acetaminophen-Induced Toxicity in the Liver and Retina, Yoshinori Sato; Wenjing Dong; Tatsuro Nakamura; Naohiro Mizoguchi; Tasuku Nawaji; Miyu Nishikawa; Takenori Onaga; Shinichi Ikushiro; Makoto Kobayashi; Hiroki Teraoka, International Journal of Molecular Sciences, MDPI AG, 24, (4) 4013 - 4013, 2023 02 16 |
| Intravenous administration of xenin-25 accelerates cyclic ruminal contractions in healthy conscious sheep., Takenori Onaga; Ami Sakai; Yumiko Yasui, Neuropeptides, 96, 102293 - 102293, 2022 12 |
| Neurotensin and xenin stimulates pancreatic exocrine secretion through the peripheral cholinergic nerves in conscious sheep., Takenori Onaga; Yumiko Yasui; Hideaki Hayashi, General and comparative endocrinology, 326, 114073 - 114073, 2022 09 15 |
| Effects of xenin-25 on insulin and glucagon secretions in healthy conscious sheep., Takenori Onaga; Hideaki Hayashi; Yumiko Yasui, Domestic animal endocrinology, 77, 106635 - 106635, 2021 10 |
| Messenger RNA expression and localization of xenin in the gastrointestinal tract in sheep, T. Onaga; A. Sakai; M. Kajita; H. Fukuda; Y. Yasui; H. Hayashi, Domestic Animal Endocrinology, Elsevier BV, 74, 106523 - 106523, 2021 01 |
| Role of neurotensin in the regulation of gastric motility in healthy conscious sheep, Takenori Onaga; Tomohiko Shimoda; Taro Ohishi; Yumiko Yasui; Hideaki Hayashi, Small Ruminant Research, Elsevier BV, 172, 31 - 41, 2019 03 |
| An Examination of Parasitic Infection and Cortisol Measurement with Feces from Captive Apes Kept in a Zoological Garden, AKIBA Yuki; KOIDE Kentaro; HIRATA Haruyuki; ONAGA Takenori; IWANO Hidetomo; YOKOTA Hiroshi; ASAKAWA Mitsuhiko; YOSHIDA Junichi; TAKAESU Noboru; ISHIBASHI Hiroki; WATANABE Yoko; TAKEDA Masahiro; ISHII Kazuko; IWATA Koichi; YAMAMOTO Tatsuya, Japanese Journal of Zoo and Wildlife Medicine, Japanese Society of Zoo and Wildlife Medicine, 23, (2) 27 - 31, 2018 |
| Characterization of feline cytochrome P450 2B6, Gaku Okamatsu; Tetsuya Komatsu; Yuka Ono; Hiroki Inoue; Tsuyoshi Uchide; Takenori Onaga; Daiji Endoh; Takio Kitazawa; Takeo Hiraga; Yasuhiro Uno; Hiroki Teraoka, XENOBIOTICA, 47, (2) 93 - 102, 2017 |
| Identification and functional characterization of novel feline cytochrome P450 2A, Gaku Okamatsu; Tetsuya Komatsu; Akira Kubota; Takenori Onaga; Tsuyoshi Uchide; Daiji Endo; Rikio Kirisawa; Guojun Yin; Hiroki Inoue; Takio Kitazawa; Yasuhiro Uno; Hiroki Teraoka, XENOBIOTICA, 45, (6) 503 - 510, 2015 06 |
| Assessment of Testicular Corticosterone Biosynthesis in Adult Male Rats, Naoyuki Maeda; Sachi Tahata; Takeshi Yagi; Emi Tanaka; Kanae Masu; Michiko Sato; Satoko Haeno; Takenori Onaga; Hiroshi Yokota, PLOS ONE, 10, (2) , 2015 02 |
| Tachykinin: Recent developments and novel roles in health and disease, Takenori Onaga, Biomolecular Concepts, De Gruyter Mouton, 5, (3) 225 - 243, 2014 06 01 |
| Polymeric immunoglobulin receptor expression and local immunoglobulin A production in bovine sublingual, submandibular and parotid salivary glands, K. Sakaguchi; H. Yokota; T. Miyasho; N. Maeda; K. Nakamura; T. Onaga; M. Koiwa; K. Matsuda; M. Okamoto; K. Hirayama; H. Taniyama, Veterinary Journal, 197, (2) 291 - 296, 2013 08 |
| Role of tachykinins and neurokinin receptor subtypes in the regulation of motility of the forestomach and abomasum in conscious sheep, Taro Oh-ishi; Tomohiko Shimoda; Hideaki Hayashi; Takenori Onaga, NEUROPEPTIDES, 47, (1) 9 - 18, 2013 02 |
| Fatty acid-binding protein expression in the gastrointestinal tract of calves and cows, Hideaki Hayashi; Sachiko Maruyama; Madoka Fukuoka; Takaharu Kozakai; Keiichi Nakajima; Takenori Onaga; Seiyu Kato, ANIMAL SCIENCE JOURNAL, 84, (1) 35 - 41, 2013 |
| A Case of Preliminary Utilization of Commercial ELISA Kit for Detecting Excretion of Urinary 8-hydroxyguanosine (8-OHdG) in Captive Chimpanzees, Pan troglodytes, Ohshima, Yuko; Ai Mizuo; Keiko Sugama; Genichi Idani; Ryoji Shimura; Akiko Uebayachi; Satoru Takahashi; Manabu Onuma; Takenori Onaga; Katsuro Hagiwara; Mutsuhiko Asakawa, Journal of Jpn. Assoc. Zoo Aqu., 日本動物園水族館協会, 52, (4) 140 - 144, 2012 04 |
| Role of tachykinin and neurokinin receptors in the regulation of ovine omasal contractions, Takenori Onaga; Taro Oh-ishi; Tomohiko Shimoda; Seira Nishimoto; Hideaki Hayashi, REGULATORY PEPTIDES, 173, (1-3) 64 - 73, 2012 01 |
| ラット小腸寄生線虫Orientostrongylus ezoensisの感染時における胃の影響, 山田 清太郎; 大西 広輔; 田澤 寿保; 石原 智明; 植田 弘美; 翁長 武紀; 北澤 多喜雄; 平田 晴之; 福本 真一郎, 獣医寄生虫学会誌, 日本獣医寄生虫学会, 9, (2) 134 - 134, 2011 03 |
| ラット小腸寄生線虫Orientostrongylus ezoensisの感染時における胃の影響, 山田 清太郎; 田澤 寿保; 石原 智明; 植田 弘美; 翁長 武紀; 北澤 多喜雄; 平田 晴之; 福本 真一郎, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 150回, 183 - 183, 2010 09 |
| Ghrelin stimulates gastric motility of the guinea pig through activation of a capsaicin-sensitive neural pathway: in vivo and in vitro functional studies, T. Nakamura; T. Onaga; T. Kitazawa, NEUROGASTROENTEROLOGY AND MOTILITY, 22, (4) 446 - +, 2010 04 |
| Role of nitrergic nerves in the regulation of motility of the omasum and abomasum in healthy sheep (Ovis aries), Takenori Onaga; Noriko Hara; Yasuhito Shimizu, VETERINARY RESEARCH COMMUNICATIONS, 33, (1) 33 - 48, 2009 01 |
| Preliminary Research on the Excretion of Urinary 8-Hydroxyguanosine (8-OHdG) as a Marker of Protozoan Parasites Infection in Captive Western Lowland Gorillas (Gorilla gorilla gorilla), MIZUO Ai; ONUMA Manabu; ONAGA Takenori; HAGIWARA Katsuro; SANADA Yoshinori; ASAKAWA Mitsuhiko; OHSHIMA Yuko; IMANISHI Ryo; KITADA Yuji; KASAHARA Michiko; HASHIZAKI Fumitaka; WADA Seitaro; MATUNAGA Masayuki; TAKAI Susumu, Japanese Journal of Zoo and Wildlife Medicine, Japanese Society of Zoo and Wildlife Medicine, 14, (1) 77 - 80, 2009 |
| Localization of CCK-1 R in the omasum and role of CCK in the regulation of ornasal contractions in sheep, Takenori Onaga; Aya Sugita; Rena Wakaiki; Ichiro Hara; Kanji Kagawa; Rikio Kirisawa; Hideaki Hayashi, DOMESTIC ANIMAL ENDOCRINOLOGY, 35, (2) 231 - 244, 2008 08 |
| Effect of intravenous infusion of proglumide on ruminal motility in conscious sheep (Ovis aries), T. Onaga, VETERINARY RESEARCH COMMUNICATIONS, 31, (8) 1021 - 1036, 2007 11 |
| Localization and secretion of epidermal growth factor in the parotid gland and its intragastric kinetics in sheep, Takenori Onaga; Yasuhito Shimizu; Hideaki Hayashi; Masayoshi Tsuji; Daiji Endoh; Hiroyuki Okada, LIFE SCIENCES, 79, (17) 1616 - 1629, 2006 09 |
| The Molecular Marker NTx Reflecting Changes in Tissue Remodeling Associated with Injury to the Equine Superficial Digital Flexor Tendon, HOSAKA Yoshinao; HIGUCHI Hidetoshi; ONAGA Takenori; NAGATA Hiroshi; KAMIKURA Tomoki; MISUMI Kazuhiro; UEDA Hiromi; Kazushige, Journal of the Japan Veterinary Medical Association, Japan Veterinary Medical Association, 58巻,第3号,p.175-179, (3) 175 - 179, 2005 |
| Multiple regulation of peptide YY secretion in the digestive tract, T Onaga; R Zabielski; S Kato, PEPTIDES, 23, (2) 279 - 290, 2002 02 |
| Effects of nitric oxide donor and nitric oxide synthase inhibitor on ruminal contractions in conscious sheep, T Onaga; H Okada; S Hagiwara; C Nagashima; H Inoue; W Korczynski; S Kato, RESEARCH IN VETERINARY SCIENCE, 71, (3) 189 - 195, 2001 12 |
| Role of peptide tyrosine tyrosine (PYY) in the regulation of gastrointestinal motility and pancreatic exocrine secretion in sheep, Takenori Onaga, Hokkaido University, Hokkaido University, 49, (1) 30 - 31, 2001 |
| Effect of nitric oxide synthase inhibitors on the temporal coordination of duodenal contractions and pancreatic exocrine secretion in sheep, T Onaga; C Nagashima; T Sakata, JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY, 170, (5-6) 469 - 479, 2000 09 |
| Regional distribution and plasma concentration of peptide YY in sheep, T Onaga; M Yoshida; H Inoue; H Yokota, PEPTIDES, 21, (5) 655 - 667, 2000 05 |
| Small intestinal UDP-glucuronosyltransferase sheUGT1A07: Partial purification and cDNA cloning from sheep small intestine, T Kobayashi; A Tatano; H Yokota; T Onaga; T Watanabe; A Yuasa, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 364, (2) 143 - 152, 1999 04 |
| Pituitary adenylate cyclase-activating polypeptide (PACAP) induces duodenal phasic contractions via the vagal cholinergic nerves in sheep, T Onaga; Y Harada; K Okamoto, REGULATORY PEPTIDES, 77, (1-3) 69 - 76, 1998 10 |
| Effects of proglumide on cholecystokinin-8-induced exocrine and endocrine pancreatic responses in conscious sheep, H. Mineo; N. Iwaki; K. Kogishi; T. Onaga; S. Kato; R. Zabielski, Comparative Biochemistry and Physiology - A Physiology, 118, (3) 759 - 764, 1997 11 |
| PACAP stimulates pancreatic exocrine secretion via the vagal cholinergic nerves in sheep, T Onaga; K Okamoto; Y Harada; H Mineo; S Kato, REGULATORY PEPTIDES, 72, (2-3) 147 - 153, 1997 10 |
| PGE(2) inhibits glucose uptake in isolated villous epithelial cells of the ovine small intestine, HS Hyun; S Arai; T Onaga; S Kato, JOURNAL OF VETERINARY MEDICINE SERIES A-PHYSIOLOGY PATHOLOGY CLINICAL MEDICINE, 44, (7) 443 - 447, 1997 09 |
| Role of peptide YY in regulation of duodenal motility during the interdigestive period in sheep, T Onaga; S Kubagawa; S Lee; H Mineo; S Kato; S Naruse; T Ozaki, JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY, 167, (5) 352 - 360, 1997 07 |
| Effects of oxytocin, arginine-vasopressin and lysine-vasopressin on insulin and glucagon secretion in sheep, H Mineo; M Ito; H Muto; H Kamita; HS Hyun; T Onaga, RESEARCH IN VETERINARY SCIENCE, 62, (2) 105 - 110, 1997 03 |
| Responses of glucose absorption and fructose absorption to prostaglandin E2 in intestinal loops of sheep, HS Hyun; T Onaga; H Mineo; S Kato, RESEARCH IN VETERINARY SCIENCE, 62, (2) 153 - 157, 1997 03 |
| Effects of C-terminal fragments of cholecystokinin on plasma insulin and glucagon concentrations in sheep, H Mineo; H Kamita; H Muto; M Ito; HS Hyun; T Onaga, RESEARCH IN VETERINARY SCIENCE, 62, (2) 171 - 174, 1997 03 |
| PACAP contracts the ovine duodenum via the vagal cholinergic nerves, T Onaga; Y Harada; K Okamoto, ADVANCES IN COMPARATIVE ENDOCRINOLOGY, TOMES 1 AND 2, 693 - 696, 1997 |
| Effect of L364 718 on interdigestive pancreatic exocrine secretion and gastroduodenal motility in conscious sheep, T Onaga; H Mineo; S Kato, REGULATORY PEPTIDES, 68, (2) 139 - 146, 1997 01 |
| Effect of cholera toxin on glucose absorption and net movements of water and electrolytes in the intestinal loop of sheep, HS Hyun; T Onaga; H Mineo; JT Kim; S Kato, JOURNAL OF VETERINARY MEDICINE SERIES A-PHYSIOLOGY PATHOLOGY CLINICAL MEDICINE, 43, (10) 611 - 618, 1996 12 |
| Comparison of the effects of secretagogues on the net movement of electrolyte and glucose absorption in the proximal and middle small intestine of sheep, HS Hyun; T Onaga; H Mineo; S Kato, JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION, 76, (4-5) 160 - 169, 1996 11 |
| Effect of pituitary adenylate cyclase-activating polypeptide on exocrine and endocrine secretion in the ovine pancreas, T Onaga; M Uchida; M Kimura; M Miyazaki; H Mineo; S Kato; R Zabielski, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY, 115, (3) 185 - 193, 1996 11 |
| Loperamide does not affect the prostaglandin E(2) (PGE(2))-induced anti-absorptive effect on glucose in the small intestinal loop of sheep, HS Hyun; T Onaga; H Mineo; S Kato, JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION-ZEITSCHRIFT FUR TIERPHYSIOLOGIE TIERERNAHRUNG UND FUTTERMITTELKUNDE, 76, (2-3) 80 - 89, 1996 09 |
| Effects of postoperative periods on glucose absorption and electrolyte secretion in the intestinal loop of sheep, HS Hyun; T Onaga; H Mineo; S Kato, JOURNAL OF VETERINARY MEDICINE SERIES A-ZENTRALBLATT FUR VETERINARMEDIZIN REIHE A-PHYSIOLOGY PATHOLOGY CLINICAL MEDICINE, 42, (9) 601 - 610, 1995 11 |
| The effect of PGE(2) on glucose absorption and net movement of water and electrolytes in the jejunal loop in sheep, HS Hyun; T Onaga; H Mineo; S Kato, JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION-ZEITSCHRIFT FUR TIERPHYSIOLOGIE TIERERNAHRUNG UND FUTTERMITTELKUNDE, 74, (4-5) 185 - 193, 1995 11 |
| Effect of neural blockades, gastrointestinal regulatory peptides, and diversion of gastroduodenal contents on periodic pancreatic secretion in the preruminant calf, R Zabielski; P Kiela; T Onaga; H Mineo; PC Gregory; S Kato, CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 73, (11) 1616 - 1624, 1995 11 |
| EFFECT OF VASOACTIVE INTESTINAL POLYPEPTIDE (VIP) ON THE NET MOVEMENT OF ELECTROLYTES AND WATER AND GLUCOSE-ABSORPTION IN THE JEJUNAL LOOP OF SHEEP, HS HYUN; T ONAGA; H MINEO; S KATO, JOURNAL OF VETERINARY MEDICAL SCIENCE, 57, (5) 865 - 869, 1995 10 |
| ROLE OF ENDOGENOUS CCK IN REGULATION OF INTERDIGESTIVE PANCREATIC EXOCRINE SECRETION IN SHEEP (OVIS-ARIES), S TACHIBANA; T ONAGA; H MINEO; S KATO, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY, 112, (1) 103 - 109, 1995 09 |
| EFFECTS OF BENZOIC-ACID AND ITS ANALOGS ON INSULIN AND GLUCAGON-SECRETION IN SHEEP, H MINEO; T OHDATE; K FUKUMURA; T KATAYAMA; T ONAGA; S KATO; N YANAIHARA, EUROPEAN JOURNAL OF PHARMACOLOGY, 280, (2) 149 - 154, 1995 07 |
| INTRADUODENAL CHOLECYSTOKININ-OCTAPEPTIDE (CCK-8) CAN STIMULATE PANCREATIC-SECRETION IN THE CALF, R ZABIELSKI; T ONAGA; H MINEO; S KATO; SG PIERZYNOWSKI, INTERNATIONAL JOURNAL OF PANCREATOLOGY, 17, (3) 271 - 278, 1995 06 |
| EFFECTS OF INTRAVENOUS INFUSIONS OF CHOLECYSTOKININ (CCK)-8 ON EXOCRINE AND ENDOCRINE PANCREATIC-SECRETION IN CONSCIOUS SHEEP, H MINEO; N IWAKI; K KOGISHI; R ZABIELSKI; T ONAGA; S KATO, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY, 111, (1) 133 - 138, 1995 05 |
| CHOLECYSTOKININ DOES NOT ACT ON THE EFFERENT PATHWAY OF CHOLINERGIC AND ADRENERGIC-NERVES TO INHIBIT RUMINAL CONTRACTIONS IN SHEEP (OVIS-ARIES), T ONAGA; T ONODERA; H MINEO; S KATO, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY, 111, (1) 51 - 58, 1995 05 |
| Effect of intravenous infusions of cholecystokinin-8 and pentagastrin on plasma concentration of insulin and glucagon in sheep, Mineo; H. Iwaki; N. Onaga; T. Kato, Research in Veterinary Science, 56, (3) 298 - 302, 1995 |
| PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE (PACAP) STIMULATES EXOCRINE PANCREAS IN CONSCIOUS PRERUMINATING CALVES, R ZABIELSKI; T ONAGA; H MINEO; E OKINE; S KATO, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 109, (1) 93 - 99, 1994 09 |
| CHEMICAL SPECIFICITY OF SHORT-CHAIN FATTY-ACIDS IN STIMULATING INSULIN AND GLUCAGON-SECRETION IN SHEEP, H MINEO; Y HASHIZUME; Y HANAKI; K MURATA; H MAEDA; T ONAGA; S KATO; N YANAIHARA, AMERICAN JOURNAL OF PHYSIOLOGY, 267, (2) E234 - E241, 1994 08 |
| EFFECTS OF PERFUSION RATE AND GLUCOSE-CONCENTRATION ON GLUCOSE-ABSORPTION IN INTESTINAL THIRY-VELLA LOOP IN SHEEP, HS HYUN; T ONAGA; H MINEO; S KATO, JOURNAL OF VETERINARY MEDICAL SCIENCE, 56, (3) 617 - 618, 1994 06 |
| EFFECTS OF INTRAVENOUS INFUSIONS OF CHOLECYSTOKININ-8 AND PENTAGASTRIN ON PLASMA-CONCENTRATIONS OF INSULIN AND GLUCAGON IN SHEEP, H MINEO; N IWAKI; T ONAGA; S KATO, RESEARCH IN VETERINARY SCIENCE, 56, (3) 298 - 302, 1994 05 |
| PLASMA SECRETIN FLUCTUATES IN-PHASE WITH PERIODIC PANCREATIC-SECRETION AND THE DUODENAL MIGRATING MYOELECTRIC COMPLEX IN CALVES, R ZABIELSKI; Y TERUI; T ONAGA; H MINEO; S KATO, RESEARCH IN VETERINARY SCIENCE, 56, (3) 332 - 337, 1994 05 |
| LOCAL VERSUS PERIPHERAL-BLOOD ADMINISTRATION OF CHOLECYSTOKININ-8 AND SECRETIN ON PANCREATIC-SECRETION IN CALVES, R ZABIELSKI; T ONAGA; H MINEO; SG PIERZYNOWSKI; S KATO, EXPERIMENTAL PHYSIOLOGY, 79, (3) 301 - 311, 1994 05 |
| Chemical specificity of short-chain fatty acids in stimulating insulin and glucagon secretion in sheep, H. Mineo; Y. Hashizume; Y. Hanaki; K. Murata; H. Maeda; T. Onaga; S. Kato; N. Yanaihara, American Journal of Physiology - Endocrinology and Metabolism, 267, (2) E234 - E241, 1994 |
| Pituitary adenylate cyclase-activating polypeptide (PACAP) stimulates exocrine pancreas in conscious preruminating calves, R. Zabielski; T. Onaga; H. Mineo; E. Okine; S. Kato, Comparative Biochemistry and Physiology. Part C: Comparative, 109, (1) 93 - 99, 1994 |
| EXOCRINE PANCREATIC RESPONSES TO CCK-8 AND VIP INFUSED DURING NSA AND RSA PHASE OF DUODENAL MMC IN CALVES - DOES PANCREATIC RESPONSIVENESS TO GI PEPTIDES FLUCTUATE PERIODICALLY, R ZABIELSKI; T ONAGA; H MINEO; S KATO, BIOMEDICAL RESEARCH-TOKYO, 15, 371 - 375, 1994 |
| THE INHIBITION OF INTERDIGESTIVE PERIODIC PANCREATIC-SECRETION BY PYY IN DOGS, T ONAGA; R ZABIELSKI; S KATO; S NARUSE; T OZAKI, BIOMEDICAL RESEARCH-TOKYO, 15, 377 - 381, 1994 |
| PERIODIC FLUCTUATIONS IN PANCREATIC-SECRETION AND DUODENAL MOTILITY INVESTIGATED IN NEONATAL CALVES, R ZABIELSKI; T ONAGA; H MINEO; S KATO, EXPERIMENTAL PHYSIOLOGY, 78, (5) 675 - 684, 1993 09 |
| 若齢犬における損傷坐骨神経の再生(共著), 日本獣医学会雑誌, 45-46, 775 - 779, 1993 |
| EFFECTS OF PERIPHERAL AND LOCAL VIP ADMINISTRATION ON SECRETION OF EXOCRINE PANCREAS AND ON ELECTRICAL AND MECHANICAL-ACTIVITY OF DUODENUM IN PRERUMINATING CALVES, R ZABIELSKI; T ONAGA; H MINEO; S KATO, BIOMEDICAL RESEARCH-TOKYO, 13, 243 - 246, 1992 |
| Effect of intravenous infusion of insulin, glucose and tolbutamide on pancreatic exocrine secretion in sheep, Kato, S; S. Matsumoto; H. Kojima; H. Mineo; T. Onaga; K. Kikuchi, Japanese Journal of Zootechnology and Sciense, Japanese Society of Animal Science, 61, (4) 360 - 365, 1990 |
| Effect of cholecystokinin octapeptide on contraction of bovine ruminal muscle, Onaga, T; S. Kato; H. Mineo; J. Ushijima, Asian-Australasian Journal of Animal Science, 2, 165 - 166, 1989 |
Books etc
| Duke's Physiology of Domestic Animals, 監訳; 鈴木, 浩悦; 澁谷, 泉; 翁長, 武紀; 田谷, 一善, 42～46章, 学窓社, 2020 03 , 9784873627724 |
| これからの乳牛群管理のためのハードヘルス学<成牛編>, 翁長 武紀, 3-1 反芻動物の栄養生理, 緑書房, 2017 12 |
| 実験で学ぶ生理学 [改訂版], 翁長 武紀, 消化吸収、腎臓、消化管平滑筋, 学窓社, 2014 03 |
| 実験で学ぶ生理学, 翁長 武紀, 消化吸収、腎臓、消化管平滑筋, 学窓社, 2009 10 |
Misc
| Ghrelin Stimulates Gastric Motility Through Activation of the Vago-Vagal Reflex Pathway in the Anesthetized Guinea-Pig, Takio Kitazawa; Takenori Onaga; Tatsuro Nakamura, GASTROENTEROLOGY, 136, (5) A534 - A534, 2009 05 |
| Ghrelin stimulates gastric motility of the anesthetized guinea-pig, Nakamura Tatsuro; Onaga Takenori; Kitazawa Takio, Journal of pharmacological sciences, 109, (1) 181 - 181, 2009 03 |
| Ghrelin stimulates gastric motility of the anesthetized guinea-pig, Tatsuro Nakamura; Takenori Onaga; Takio Kitazawa, JOURNAL OF PHARMACOLOGICAL SCIENCES, 109, 2009 |
| ROLE OF VIP IN THE REGULATION OF OMASAL MOTILITY IN SHEEP, Takenori Onaga; Yuji Takayama; Ei-ichi Tsuyama; Kanji Kagawa, JOURNAL OF PHYSIOLOGICAL SCIENCES, 59, 2009 |
| ヒツジにおける延髄のPACAP結合部位と上部消化管機能の調節, 翁長武紀; 石橋寛子; 遠藤大二; 保坂善真; 竹花一成, 日本獣医学会学術集会講演要旨集, 139th, 2005 |
Research Grants & Projects
| 日本学術振興会, 科学研究費助成事業, 内分泌細胞に富む消化管オルガノイドの開発と消化管ホルモンの分泌調節因子の解明, 20210401, 20240331, 翁長 武紀, オルガノイドの開発において、Xenin内分泌細胞は生体内では胃および十二指腸粘膜に高密度に局在することから、先ずラットの胃粘膜をカルシウムキレート法で処理して陰窩を含む上皮細胞を分離してマトリゲル内で3次元培養した。これにWnt,、Noggin、R-spomdin、上皮成長因子EGFや線維芽細胞成長因子FGF、ガストリンなどの各種分化促進因子を適用し、初期培養液中に必要なニコチンアミドやRHOK-inhibitorであるY-27632をある時期以降から減らすとオルガノイド形成が進み、内分泌細胞への分化も増えると報告されているが、実際行ったところその通りの結果が得られていない。1回目は約4週間、2回目は約6週間培養しているが、細胞は継代できても、陰窩の幹細胞が分化して、球形のオルガノイドを形成するに至っていない。動物種は異なるが、既報に従った組成の培養液を調製しているが、ラットの胃上皮培養細胞の分化が進まず、初年度の目標であった転写因子を遺伝子導入して内分泌細胞に富むオルガノイドを作製するところまで進んでいない。 培養細胞の分化の進行を確認するための複数のPCR系は別途準備できているが、培養細胞の形態的な分化が不十分であるので、分化を検証するに至っていない。 調節遺伝子のクローニングについては、オルガノイドにノックインするラットの転写因子のうち、十二指腸への分化に関わるラットPdx1はクローニングできたが、胃の分化に関わるラットのBapx1のクローニングが一部未完成である。 |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Ghrelin peptides induced contractions and growth hormone secretagogue receptor expression in the chicken gastrointestinal tract, 2004, 2006, KITAZAWA Takio; ONAGA Takenori; IWANO Hidetomc, Background and aim. Ghrelin is the endogenous ligand for the growth hormone secretagogue receptor (GHS-R), and stimulates food intake and gastrointestinal (GI) motility in mammals. In chickens, on the other hand, ghrelin inhibits food intake. This discrepancy prompted us to investigate whether ghrelin affects gastrointestinal motility in the chicken. Methods, The contractile effect of chicken ghrelin was measured isometrically in muscle strips from different regions of the GI tract suspended in an organ bath. The effect of chicken ghrelin was compared with that of rat or human ghrelin. In addition, mRNA expression of ghrelin and GHS-R was examined by quantitative RT-PCR. Results. Among the ghrelin peptides tested (rat, human and chicken ghrelin), only chicken ghrelin caused contraction of muscle strips from the GI tract. No effect was seen in des-octanoylated chicken ghrelin, suggesting that octanoylation at Ser^3 residue was essential for its activity. The contractile response to chicken ghrelin in the crop was not affected by tetrodotoxin (TTX), but that in the proventriculus was decreased by TTX and atropine to the same extent. D-Lys^3-GHRP-6 (a GHS-R antagonist) caused a transient contraction and inhibited the chicken ghrelin-induced contraction without affecting the 50 mM high-K^+-induced contraction. The amplitude of the chicken ghrelin-induced contraction was different among GI regions: highest in the crop and colon (70% of high-K^+-induced contraction), moderate in the esophagus and proventriculus (30%), and weak in the small intestine (10-20%) and the ranking order was consistent with GHS-R mRNA expression in each region (colon > crop = esophagus > proventriculus > duodenum =jejunum > ileum). GHS-R mRNA expression in the muscle preparations of crop and proventriculus without mucosa was 2-4 times higher than that in whole segments of crop and proventriculus, indicating that the GHS-R is mainly expressed in smooth muscle layers including enteric nerves. In contrast to the GHS-R expression, ghrelin mRNA was mainly expressed in the mucosa of proventriculus and duodenum. Conclusion. These results indicate that ghrelin stimulates GI contractility and preferentially affects the motor activity of esophagus, crop and colon through myogenic and/or neurogenic mechanisms. These actions are mediated through the GHS-R, which is highly sensitive to chicken ghrelin |
| 日本学術振興会, 科学研究費助成事業, 反芻動物における上皮増殖因子の分泌と胃内動態の研究, 2001, 2002, 翁長 武紀, 昨年度はヒツジのEGF量を測定するラジオレセプターアッセイ(RRA)を確立して、ヒツジの唾液腺におけるEGFの分布と組織含量を調べ、およびEGF活性物質の部分精製を行ったが、今年度はEGFの唾液中の分泌動態および胃内動態を検討した。方法として、手術によりヒツジの耳下腺管に挿管した後、無麻酔下において耳下腺から分泌された唾液を採取してRRAにより分泌動態を調べ、集めた唾液を低圧液体クロマトグラフィーおよびHPLCにより分析して下記の結果を得た。1)唾液腺から分離した高分子型のEGF活性物質は耳下腺の唾液中には検出されず、分子量およそ6kDaの低分子型EGFが検出された。2)食間期(10:00-18:00)の耳下腺唾液におけるEGFの濃度は582.0±33.26pM、分泌量は0.40±0.03pmol/minであり、摂食時(18:00-19:00)に濃度が1015.5±102.47pM、分泌量が2.30±0.40pmol/minに増加した。さらに胃に挿管して胃液を採取し、そのEGF濃度を調べたところ、3)第一胃液中のEGF濃度は食間期で73.3±6.0pMであり、食後に339.1±125.4pMに増加した。4)第四胃液中のEGF濃度は食間期で659.2±120.0pMであり、食後に259.4±157.3nMにまで著しく増加した。以上の結果より、唾液腺組織中の高分子型のEGF活性物質はおそらくEGFの前駆物質であり、ヒツジでは食間期においても唾液中に低分子量の分泌型EGFが常に分泌されていることが明らかとなった。その分泌は食後に著しく増加し、それに一致して第一胃内のEGF濃度も増加することが明らかとなった。また、第四胃液内のEGF濃度も食後に増加するが、第一胃よりも濃度上昇が著しいことから、唾液腺以外からの分泌によって第四胃内のEGF濃度が上昇することが示唆された。 |
| 反芻動物の消化管運動の調節機序に関する研究, 1992 |
| 消化管機能のペプチド作動性調節機序に関する研究, 1992 |
| Study on the regulatory mechanism of gastrointestinal motility in ruminants, 1992 |
| Study on peptidergic regulations of gastrointestinal functions, 1992 |
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