School of Veterinary Medicine, Department of Veterinary Medicine
村松康和 ムラマツ ヤスカズ
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Name
MURAMATSU Yasukazu
Affiliation (Master)
School of Veterinary Medicine, Department of Veterinary Medicine
Association Memberships
JAPANESE SOCIETY FOR BACTERIOLOGY THE JAPANESE ASSOCIATION FOR INFECTIOUS DISEASES 人と動物の共通感染症研究会 THE JAPANESE SOCIETY FOR VIROLOGY JAPAN VETERINARY MEDICAL ASSOCIATION THE JAPANESE SOCIETY OF VETERINARY SCIENCE
Research Activities
Research Areas
Life sciences, Veterinary medicine
Published Papers
The Evaluation of Zika virus susceptibility in Aedes (Ochlerotatus) japonicus from Hokkaido, Japan., Leo Uchida; Kana Iwamoto; Shino Kawae; Makoto Shimooka; Ronald Enrique Morales-Vargas; Yuki Eshita; Yasuko Orba; Hirofumi Sawa; Yasukazu Muramatsu, Japanese journal of infectious diseases, 2026 04 30 Detection of haemosporidian parasite lineages from wild bird carcasses collected in eastern Japan., Momoko Eki; Mizuki Sasaki; Yasukazu Muramatsu; Mari Kobayashi; Takanori Ishikawa; Leo Uchida, The Journal of veterinary medical science, 87, (7) 838 - 842, 2025 07 11 Structure and milk hygiene of dairy cooperative value chains in an intensive production area of Uganda—A bottleneck of intervention, Yoshiharu Sugino; James Bugeza; David Bahame; Joseph Byaruhanga; Haruka Shimazaki; Masahiko Anzai; Taishi Kayano; William Mwebembezi; Andrew Akashaba; Taku Shimada; Yasukazu Muramatsu; Kohei Makita, Frontiers in Sustainable Food Systems, 7, 2023 Identification of three novel genes in Phenuiviridae detected from Aedes mosquitoes in Hokkaido, Japan., Leo Uchida; Yoshimi Sakurai; Makoto Shimooka; Ronald Enrique Morales-Vargas; Katsuro Hagiwara; Yasukazu Muramatsu, Japanese journal of infectious diseases, 76, (1) 55 - 63, 2022 09 30 3種のヤブカ体内において選択されたジカウイルスのゲノム変異評価, 下岡 誠; 三木 要; 江下 優樹; 大場 靖子; 澤 洋文; 村松 康和; 内田 玲麻, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 165回, [F1P - 06], 2022 09 Cryptosporidium sp. Mrb001 detected from Myodes rufocanus bedfordiae, an indigenous vole of Hokkaido, Japan, Yasukazu Muramatsu; Leo Uchida; Yutaka Tamura; Mitsuhiko Asakawa, J. Rakuno Gakuen Univ., 2022 03 Isolation and Characterization of Mosquito-Associated Spiroplasma cantharicola from Aedes japonicus Collected in Hokkaido, Japan., Makoto Shimooka; Yoshimi Sakurai; Yasukazu Muramatsu; Leo Uchida, Insects, 12, (12) , 2021 11 25 飼い主と飼育犬における口腔内細菌の検出頻度と細菌叢の比較, 村松 康和; 井谷 寛; 野村 美月; 内田 玲麻; 郡山 尚紀, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 164回, [FO - 53], 2021 09 Zika Virus Potential Vectors among Aedes Mosquitoes from Hokkaido, Northern Japan: Implications for Potential Emergence of Zika Disease., Leo Uchida; Miki Shibuya; Ronald Enrique Morales-Vargas; Katsuro Hagiwara; Yasukazu Muramatsu, Pathogens (Basel, Switzerland), 10, (8) , 2021 07 24 Effect of chemical tick control practices on tick infestation and Theileria parva infection in an intensive dairy production region of Uganda., Miyama, T; Byaruhanga J; Okamura, I; Uchida, L; Muramatsu, Y; Mwebembezi, W; Vudriko, P; Makita, K, Ticks Tick Borne Dis., Elsevier BV, 11, (4) 101438 - 101438, 2020 07 Prevalence of sub-clinical mastitis and its association with milking practices in an intensive dairy production region of Uganda., Miyama, T; Byaruhanga J; Okamura, I; Nagahata, H; Murata, R; Mwebembezi, W; Muramatsu, Y; Makita, K, J Vet Med Sci, Japanese Society of Veterinary Science, 82, (4) 488 - 493, 2020 04 FTA-Sodium hydroxide-based polymerase chain reaction (PCR): An efficient and cheaper option for Theileria parva detection in dairy cattle in Mbarara, Uganda., Leo Uchida; Joseph Byaruhanga; Ikuo Okamura; Takeshi Miyama; Yasukazu Muramatsu; Patrick Vudriko; Kohei Makita, The Journal of veterinary medical science, 82, (2) 188 - 192, 2020 02 18 PCR PRIMER DESIGN METHOD FOR DIFFERENTIATING AMONG SALMONELLA SEROGROUPS BASED ON AN ALGORITHM TARGETING GENE-FLANKING REGIONS, Yasukazu MURAMATSU, The Southeast Asian Journal of Tropical Medicine and Public Health, 50, (5) , 2019 09 Bergeyella zoohelcum isolated from oral cavities of therapy dogs, Yasukazu MURAMATSU; Nami HARAYA; Kazuki HORIE; Leo UCHIDA; Takanori KOORIYAMA; Akio SUZUKI; Motohiro HORIUCHI, Zoonoses and Public Health, 2019 08 MALDI Biotyperを用いた豚の肺膿瘍における網羅的細菌解析(Comprehensive identification of bacteria in pig lung abscesses using the MALDI Biotyper system), 内田 玲麻; 中山 大輔; 黒澤 拓也; Tran Nhat T.; 村田 亮; 宮庄 拓; 山 奈津子; 村松 康和, 酪農学園大学紀要(自然科学編), 酪農学園大学, 43, (2) 85 - 96, 2019 03 MALDI Biotyperを用いた健康牛の乳汁中における細菌の解析, 惠 之巍; 村田 亮; 大塚 浩通; 内田 玲麻; 村松 康和, 家畜感染症学会誌, 家畜感染症学会誌編集委員会, 7, (4) 181 - 181, 2018 12 Survey of tick-borne zoonotic viruses in wild deer in Hokkaido, Japan., Leo Uchida; Daisuke Hayasaka; Mya Myat Ngwe Tun; Kouichi Morita; Yasukazu Muramatsu; Katsuro Hagiwara, The Journal of veterinary medical science, 80, (6) 985 - 988, 2018 06 29 Genotyping of Coxiella burnetii detected from bovine placenta based on Multiple-locus variable-number tandem repeat analysis, Yasukazu MURAMATSU, Research of One Health, 2016 12 液体クロマトグラフ質量分析計(LC/MS)を用いた節足動物媒介性ウイルスの探索, 内田 玲麻; 岡本 健太; 鍋島 武; 青木 康太郎; 村松 康和; 森田 公一; 長谷部 太, 北海道獣医師会雑誌, (公社)北海道獣医師会, 60, (8) 427 - 427, 2016 08 Detection of methicillin-resistant Staphylococcus pseudintermedius ST169 and novel ST354 SCCmec II-III isolates related to the worldwide ST71 clone, K. Ishihara; A. Koizumi; M. Saito; Y. Muramatsu; Y. Tamura, Epidemiology and Infection, Cambridge University Press, 144, (2) 434 - 442, 2016 01 01 Epidemiological Analysis of Methicillin-Resistant Staphylococcus aureus Carriage among Veterinary Staff of Companion Animals in Japan, Kanako Ishihara; Mieko Saito; Natsumi Shimokubo; Yasukazu Muramatsu; Shigeki Maetani; Yutaka Tamura, JOURNAL OF VETERINARY MEDICAL SCIENCE, 76, (12) 1627 - 1629, 2014 12 SEROEPIDEMIOLOGIC SURVEY IN THAILAND OF COXIELLA BURNETII INFECTION IN CATTLE AND CHICKENS AND PRESENCE IN TICKS ATTACHED TO DAIRY CATTLE, Yasukazu Muramatsu; Noriyo Usaki; Chalermchaikit Thongchai; Indhira Kramomtong; Poonsuk Kriengsak; Yutaka Tamura, SOUTHEAST ASIAN JOURNAL OF TROPICAL MEDICINE AND PUBLIC HEALTH, 45, (5) 1167 - 1172, 2014 09 Genetic Diversity in the Prion Protein Gene (PRNP) of Domestic Cattle and Water Buffaloes in Vietnam, Indonesia and Thailand, Leo Uchida; Agus Heriyanto; Chalermchaikit Thongchai; Tran Thi Hanh; Motohiro Horiuchi; Kanako Ishihara; Yutaka Tamura; Yasukazu Muramatsu, JOURNAL OF VETERINARY MEDICAL SCIENCE, 76, (7) 1001 - 1008, 2014 07 Antimicrobial Susceptibility of Indicator Bacteria Isolated from Chickens in Southeast Asian Countries (Vietnam, Indonesia and Thailand), Masaru Usui; Shuhei Ozawa; Hiroyuki Onozato; Rikiya Kuge; Yuko Obata; Tomoko Uemae; Pham Thi Ngoc; Agus Heriyanto; Tongchai Chalemchaikit; Kohei Makita; Yasukazu Muramatsu; Yutaka Tamura, JOURNAL OF VETERINARY MEDICAL SCIENCE, 76, (5) 685 - 692, 2014 05 Methicillin-resistant Staphylococcus aureus carriage among veterinary staff and dogs in private veterinary clinics in Hokkaido, Japan, Kanako Ishihara; Mieko Saito; Natsumi Shimokubo; Yasukazu Muramatsu; Shigeki Maetani; Yutaka Tamura, MICROBIOLOGY AND IMMUNOLOGY, 58, (3) 149 - 154, 2014 03 Distribution of antimicrobial-resistant lactic acid bacteria in natural cheese in Japan, Kanako Ishihara; Kumiko Nakajima; Satoko Kishimoto; Fumiaki Atarashi; Yasukazu Muramatsu; Akitoyo Hotta; Satomi Ishii; Yasuyuki Takeda; Masanori Kikuchi; Yutaka Tamura, Microbiology and Immunology, 57, (10) 684 - 691, 2013 10 Contribution of the AcrAB-TolC efflux pump to high-level fluoroquinolone resistance in Escherichia coli isolated from dogs and humans, Toyotaka Sato; Shin-Ichi Yokota; Torahiko Okubo; Kanako Ishihara; Hiroshi Ueno; Yasukazu Muramatsu; Nobuhiro Fujii; Yutaka Tamura, Journal of Veterinary Medical Science, 75, (4) 407 - 414, 2013 Factors associated with antimicrobial-resistant Escherichia coli in zoo animals, Kanako Ishihara; Yuko Hosokawa; Kohei Makita; Jun Noda; Hiroshi Ueno; Yasukazu Muramatsu; Hiroshi Ueno; Takeshi Mukai; Hideaki Yamamoto; Masaki Ito; Yutaka Tamura, RESEARCH IN VETERINARY SCIENCE, 93, (2) 574 - 580, 2012 10 Parasitic Nematodes from Albino Individuals of Japanese Rat snakes Elaphe climacophora in Yamaguchi Prefecture, Japan, MATSUDA, Kazuya; MURAMATSU, Yasukazu; ASAKAWA, Mitsuhiko, 獣医畜産新報, 文永堂出版, 65, (9) 753 - 756, 2012 09 Effects of climatic elements on Campylobacter-contaminated chicken products in Japan., Ishihara K; Takahashi R; Andoh M; Makita K; Kamiji S; Ueno H; Muramatsu Y; Tamura Y, Epidemiol Infect, Cambridge University Press, 140, (6) 991 - 6, 2012 06 高速相同性検索ソフトUSEARCHを用いた乳牛糞便DNA-Illumina分析データのメタゲノム解析, 遠藤 大二; 北市 みのり; 村松 康和; 杉村 逸郎; 林 正信, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 153回, 238 - 238, 2012 03 Seasonal Variation in Campylobacter-contaminated Retail Chicken Products: A Year-Round Investigation in Japan, Kanako Ishihara; Ryuta Takahashi; Masako Andoh; Hiroshi Ueno; Yasukazu Muramatsu; Yutaka Tamura, JOURNAL OF VETERINARY MEDICAL SCIENCE, 74, (1) 117 - 120, 2012 01 Antimicrobial Susceptibility of Escherichia coli Isolates from Wild Mice in a Forest of a Natural Park in Hokkaido, Japan, Kanako Ishihara; Kyoko Kanamori; Tetsuo Asai; Akemi Kojima; Toshio Takahashi; Hiroshi Ueno; Yasukazu Muramatsu; Yutaka Tamura, JOURNAL OF VETERINARY MEDICAL SCIENCE, 73, (9) 1191 - 1193, 2011 09 鶏肉のカンピロバクター汚染に対する気候要素の影響, 石原 加奈子; 高橋 隆太; 安藤 匡子; 蒔田 浩平; 上野 弘志; 村松 康和; 田村 豊, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 152回, 260 - 260, 2011 08 カンピロバクターによる畜産食品汚染の季節変動, 石原 加奈子; 高橋 隆太; 安藤 匡子; 上野 弘志; 村松 康和; 田村 豊, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 151回, 243 - 243, 2011 03 Occurrence and Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus and Methicillin-Resistant Staphylococcus pseudintermedius in an Academic Veterinary Hospital, Kanako Ishihara; Natsumi Shimokubo; Akie Sakagami; Hiroshi Ueno; Yasukazu Muramatsu; Tsuyoshi Kadosawa; Chie Yanagisawa; Hideaki Hanaki; Chie Nakajima; Yasuhiko Suzuki; Yutaka Tamura, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76, (15) 5165 - 5174, 2010 08 Availability of Portable Endotoxin Test System in Commercial Milk and Milk Drinks, SAKEMI Yoko; TAKAHASHI Junkichi; FUNATSUKI Kiyomi; ISHIHARA Kanako; UENO Hiroshi; MURAMATSU Yasukazu; TAMURA Yutaka, Journal of the Japan Veterinary Medical Association, Japan Veterinary Medical Association, 63, (2) 145 - 147, 2010 02 20 犬由来大腸菌のフルオロキノロン耐性機構, 佐藤 豊孝; 石原 加奈子; 村松 康和; 上野 弘志; 岡林 環樹; 横田 伸一; 藤井 暢弘; 田村 豊, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 146回, 319 - 319, 2008 09 Frequencies of PRNP gene polymorphisms in Vietnamese dairy cattle for potential association with BSE, Y. Muramatsu; Y. Sakemi; M. Horiuchi; T. Ogawa; K. Suzuki; M. Kanameda; T. T. Hanh; Y. Tamura, Zoonoses and Public Health, 55, (5) 267 - 273, 2008 06 動物病院におけるメチシリン耐性黄色ブドウ球菌の検出, 坂上 亜希恵; 村松 康和; 上野 弘志; 廉澤 剛; 田村 豊, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 144回, 124 - 124, 2007 08 Seroepidemiologic survey of Coxiella burnetii and attempt to detect Coxiella DNA in aged non-laying chickens in a prefecture of Japan where poultry farming prospers, Yasukazu Muramatsu; Kikuto Hukuta; Sayaka Satoh; Masatake Muramatsu; Masaaki Nishimura; Hajime Nagahata; Hiroshi Ueno; Chiharu Morita; Yutaka Tamura, JOURNAL OF VETERINARY MEDICAL SCIENCE, 68, (9) 1007 - 1008, 2006 09 Organizing pneumonia associated with Q fever, ONOE Kazuyuki; NASUHARA Yasuyuki; KASAHARA Ikumi; HIZAWA Nobuyuki; NISHIMURA Masaharu; UENO Hiroshi; MURAMATSU Yasukazu; MORITA Chiharu; TAMURA Yutaka, Nihon Naika Gakkai Kaishi, The Japanese Society of Internal Medicine, 94, (5) 961 - 963, 2005 05 10 Detection of Ehrlichial DNA in small rodents captured in a woodland area of Hokkaido, the northernmost island of Japan, where lyme disease is endemic, Yasukazu Muramatsu; Emiko Ikeda; Chiharu Morita; Yutaka Tamura, Japanese Journal of Infectious Diseases, 58, (5) 316 - 319, 2005 Spotted fever group rickettsiae from ticks captured in Sudan, Chiharu Morita; Abdel Rahim Mohamed El Hussein; Etsurou Matsuda; Khalil Mohamed Ali Abdel Gabbar; Yasukazu Muramatsu; Magdi Badawi Abdel Rahman; Ali Mohamed Hassan Eleragi; Shawgi Mohamed Hassan; Agnes Mumbi Chitambo; Hiroshi Ueno, Japanese Journal of Infectious Diseases, 57, (3) 107 - 109, 2004 06 Experimental transmission of abnormal prion protein (PrPsc) in the small intestinal epithelial cells of neonatal mice, M. Okamoto; H. Furuoka; M. Horiuchi; T. Noguchi; K. Hagiwara; Y. Muramatsu; K. Tomonaga; M. Tsuji; C. Ishihara; K. Ikuta; H. Taniyama, Veterinary Pathology, 40, (6) 723 - 727, 2003 11 Characterization of spotted fever group rickettsiae detected in dogs and ticks in Okinawa, Japan, Hiroshi Satoh; Yurie Motoi; Gerry A. Camer; Hisashi Inokuma; Masako Izawa; Tsuyoshi Kiyuuna; Norichika Kumazawa; Yasukazu Muramatsu; Hiroshi Ueno; Chiharu Morita, Microbiology and Immunology, 46, (4) 257 - 263, 2002 01 01 First detection of Ehrlichia platys in dogs and ticks in Okinawa, Japan, Yurie Motoi; Hiroshi Satoh; Hisashi Inokuma; Tsuyoshi Kiyuuna; Yasukazu Muramatsu; Hiroshi Ueno; Çhiharu Morita, Microbiology and Immunology, 45, (1) 89 - 91, 2001 01 01 Seroprevalence of antibodies against spotted fever group Rickettsia among dogs and humans in Okinawa, Japan, Hiroshi Satoh; Akemi Tsuneki; Hisashi Inokuma; Norichika Kumazawa; Yoshihide Jahana; Tsuyoshi Kiyuuna; Tamaki Okabayashi; Yasukazu Muramatsu; Hiroshi Ueno; Chiharu Morita, Microbiology and Immunology, Center for Academic Publications Japan, 45, (1) 85 - 87, 2001 Detection of serum antibodies to Bartonella henselae and Coxiella burnetii from Japanese children and pregnant women, Kei Numazaki; Hiroshi Ueno; Kazuko Yokoo; Yasukazu Muramatsu; Shunzo Chiba; Chiharu Morita, Microbes and Infection, Elsevier Masson SAS, 2, (12) 1431 - 1434, 2000 Serosurvey of wild rodents for Rickettsioses (spotted fever, murine typhus and Q fever) in Java Island, Indonesia, Ima Nurisa Ibrahim; Tamaki Okabayashi; Ristiyanto; Enny Wahyu Lestari; Tsuyoshi Yanase; Yasukazu Muramatsu; Hiroshi Ueno; Chiharu Morita, European Journal of Epidemiology, 15, (1) 89 - 93, 1999 Short report: Prevalence of antibodies against spotted fever, murine typhus, and Q fever rickettsiae in humans living in Zambia, Tamaki Okabayashi; Futoshi Hasebe; Kenny L. Samui; Aaron S. Mweene; Shanker G. Pandey; Tsuyoshi Yanase; Yasukazu Muramatsu; Hiroshi Ueno; Chiharu Morita, American Journal of Tropical Medicine and Hygiene, American Society of Tropical Medicine and Hygiene, 61, (1) 70 - 72, 1999 Detection of Coxiella burnetii from dust in a barn housing dairy cattle, Tsuyoshi Yanase; Yasukazu Muramatsu; Iwao Inouye; Tamaki Okabayashi; Hiroshi Ueno; Chiharu Morita, Microbiology and Immunology, Blackwell Publishing Asia, 42, (1) 51 - 53, 1998 Detection of coxiella burnetii in cow's milk by PCR-enzyme-linked immunosorbent assay combined with a novel sample preparation method, Yasukazu Muramatsu; Tsuyoshi Yanase; Tamaki Okabayashi; Hiroshi Ueno; Chiharu Morita, Applied and Environmental Microbiology, 63, (6) 2142 - 2146, 1997 06 Seasonal variations in the presence of antibodies against Coxiella burnetii in dairy cattle in Hokkaido, Japan, Tsuyoshi Yanase; Yasukazu Muramatsu; Hiroshi Ueno; Chiharu Morita, Microbiology and Immunology, Blackwell Publishing Asia, 41, (2) 73 - 75, 1997 Seroepidemiological survey of lymphocytic choriomeningitis virus in wild house mice in China with particular reference to their subspecies, Chiharu Morita; Kimisachi Tsuchiya; Hiroshi Ueno; Yasukazu Muramatsu; Akiko Kojimahara; Hitoshi Suzuki; Nobumoto Miyashita; Kazuo Moriwaki; Mei-Lei Jin; Xiang-Lin Wu; Feng-Shan Wang, Microbiology and Immunology, Blackwell Publishing Asia, 40, (4) 313 - 315, 1996 Serological survey of spotted fever group rickettsia in wild rats in thailand in the 1970s, Tamaki Okabayashi; Kimiyuki Tsutiya; Yasukazu Muramatsu; Hiroshi Ueno; Chiharu Morita, Microbiology and Immunology, Blackwell Publishing Asia, 40, (12) 895 - 898, 1996 Improved method for preparation of samples for the polymerase chain reaction for detection of Coxiella burnetii in milk using immunomagnetic separation, Yasukazu Muramatsu; Minoru Maruyama; Tsuyoshi Yanase; Hiroshi Ueno; Chiharu Morita, Veterinary Microbiology, Elsevier B.V., 51, (1-2) 179 - 185, 1996 The prevalence of Listeria monocytogenes in the environment of dairy farms, Hiroshi Ueno; Keiichi Yokota; Takanori Arai; Yasukazu Muramatsu; Hiroyuki Taniyama; Takashi Iida; Chiharu Morita, Microbiology and Immunology, Blackwell Publishing Asia, 40, (2) 121 - 124, 1996 Seroepidemiological survey of wild rats for Seoul virus in Indonesia, Ima-Nurisa Ibrahim; Mohammad Sudomo; Chiharu Morita; Sachiko Uemura; Yasukazu Muramatsu; Hiroshi Ueno; Takashi Kitamura, Japanese Journal of Medical Science and Biology, National Institute of Health, 49, (2) 69 - 74, 1996 Epidemiological survey of small rodents for spotted fever rickettsial antibody in Hokkaido, Japan, Tamaki Okabayashi; Nozomi Miura; Singo Miyazaki; Tomomi Nigo; Yasukazu Muramatsu; Hiroshi Ueno; Chiharu Morita, Japanese Journal of Medical Science and Biology, National Institute of Health, 49, (2) 63 - 68, 1996 Does coinfection of Bartonella henselae and FIV induce clinical disorders in cats?, Hiroshi Ueno; Tsutomu Hohdatsu; Yasukazu Muramatsu; Hiroyuki Koyama; Chiharu Morita, Microbiology and Immunology, Blackwell Publishing Asia, 40, (9) 617 - 620, 1996 Seroepidemiological Survey of Bartonella (Rochalimaea) henselae in Domestic Cats in Japan, Hiroshi Ueno; Yasukazu Muramatsu; Chiharu Morita; Bruno B. Chomel; Tsutomu Hohdatsu; Hiroyuki Koyama, Microbiology and Immunology, 39, (5) 339 - 341, 1995 Amino acid polymorphisms of PrP with reference to onset of scrapie in Suffolk and Corriedale sheep in Japan, T. Ikeda; M. Horiuchi; N. Ishiguro; Y. Muramatsu; G. D. Kai-Uwe; M. Shinagawa, Journal of General Virology, Microbiology Society, 76, (10) 2577 - 2581, 1995 Detection of PrPSc in sheep at the preclinical stage of scrapie and its significance for diagnosis of insidious infection, Y. Muramatsu; A. Onodera; M. Horiuchi; N. Ishiguro; M. Shinagawa, Archives of Virology, Springer-Verlag, 134, (3-4) 427 - 432, 1994 09 Seroepidemiological Survey of Coxiella burnetii in Domestic Cats in Japan, Chiharu Morita; Tsutomu Hohdatsu; Hiroyuki Koyama; Jun Katsuyama; Tsuyoshi Yanase; Hiroshi Ueno; Yasukazu Muramatsu, Microbiology and Immunology, 38, (12) 1001 - 1003, 1994 The Significance of PrPSc Detection for the Diagnosis of Insidious Scrapie, Y. MURAMATSU; A. ONODERA; M. HORIUCHI; N. ISHIGURO; M. SHINAGAWA, Annals of the New York Academy of Sciences, 724, (1) 347 - 349, 1994 Isolation of Scrapie Agent from the Placenta of Sheep with Natural Scrapie in Japan, Onodera Takashi; Ikeda Toshio; Muramatsu Yasukazu; Shinagawa Morikazu, Japanese Journal of Microbiology, Center For Academic Publications Japan, 37, (4) 311 - 316, 1993 A specific RFLP type associated with the occurrence of sheep scrapie in Japan, Y. Muramatsu; K. Tanaka; M. Horiuchi; N. Ishiguro; M. Shinagawa; T. Matsui; T. Onodera, Archives of Virology, Springer-Verlag, 127, (1-4) 1 - 9, 1992 03
Books etc
公衆衛生学 ; 動物看護関連法規 ; 動物愛護・適正飼養関連法規, 日本動物保健看護系大学協会カリキュラム委員会, 第3章第1節、第4節, EDUWARD Press, 2022 02 , 9784866711591 公衆衛生学 : 動物医療関連法規, 日本動物保健看護系大学協会カリキュラム委員会, 第3章第1節、第4節, インターズー, 2019 12 , 9784866710891 動物公衆衛生学 改訂版, 村松 康和, インターズー, 2019 12 Q fever, Yasukazu MURAMATSU, 日本小児医事出版社, 2017 12 獣医公衆衛生研究, 村松 康和, 獣医大学からのお便り③, 全国公衆衛生獣医師協議会, 2017 03 化学療法の領域, 村松 康和, Q熱, 医薬ジャーナル社, 2017 02 動物公衆衛生学, 村松 康和, pp. 45-48, 89-98, 109-113., インターズー, 2015 12 健康教室, 村松 康和, ペット由来の感染症 pp.25-30., 東山書房, 2015 11 獣医公衆衛生学Ⅱ, 村松 康和, 紅斑熱群 pp59-60. Q熱 pp.86-88., 文永堂出版, 2014 05
Conference Activities & Talks
Whole-genome analysis of two novel Coxiella burnetii strains isolated from different bovine breeds., 成田駿; 小野寺杏佳; 内田玲麻; 村松康和, 第72回日本ウイルス学会学術集会 乳牛および肉牛由来Coxiella burnetii株の分離, 小野寺杏佳; 成田駿; 内田玲麻; 村松康和, 第168回日本獣医学会学術集会 Characterization and serological test of Aedes galloisi flavivirus (AGFV) and AGFV-like virus isolated from mosquitoes in Hokkaido, ○富久藍; 蒔田凪; Vincen; Ross Vicencio; 村松康和; 内田玲麻, The 71st Annual Meeting of the Japanese Society for Virology The effects of the amino acid polymorphisms surrounding the N-linked glycosylation site of ZIKA E protein on the adaptation to mosquitoes, ○島崎晴輝; 下岡誠 Vincen; Ross Vicencio; 村松康和; 内田玲麻, The 71st Annual Meeting of the Japanese Society for Virology セラピー犬の口腔内から分離されたBergeyella zoohelcumの薬剤感受性調査, 野口桃子; 平澤朋卯子; 内田玲麻; 村松康和, 第167回 日本獣医学会学術集会 セラピー犬由来Bergeyella zoohelcum分離株の薬剤感受性に関する疫学調査, 野口桃子; 平澤朋卯子; 内田玲麻; 村松康和, 令和6年度日本獣医公衆衛生学会 北海道地区学会 黒毛和牛からのCoxiella burnetiiの検出とその遺伝子型, 谷田隆太郎; 西寒水将; 片岡妃奈; 内田玲麻; 村松康和, 令和5年度日本獣医公衆衛生学会 北海道地区学会 牛飼育環境からのCoxiella burnetii の検出と遺伝子型別, 星山弘行; 内田玲麻; 村松康和, 第164回日本獣医学会学術集会案内 北海道産ヤマトヤブカにおけるジカウイルス感受性の評価, 川江 詩乃; 岩本 夏奈; 江下 優樹; 大場 靖子; 澤 洋文; 村松 康和; 内田 玲麻, 第164回日本獣医学会学術集会 北海道のヤブカにおけるアルボウイルスの検索, 内田 玲麻; 櫻井 愛美; 村松 康和, 第164回日本獣医学会学術集会 ヤマトヤブカ体内におけるジカウイルスのQuasispecies評価, 三木 要; 岩本 夏奈; 江下 優樹; 大場 靖子; 澤 洋文; 村松 康和; 内田 玲麻, 第164回日本獣医学会学術集会 飼い主と飼育犬における口腔内細菌の検出頻度と細菌叢の比較, ○村松 康和、井谷 寛、野村 美月、内田 玲麻、郡山 尚紀, 第164回日本獣医学会学術集会 「人の生活に身近な野生動物、伴侶動物と共生する微生物」, 村松康和, （一社）札幌市小動物獣医師会 野生動物委員会主催セミナー セラピードッグとその飼い主における口腔内細菌叢の比較, 西川泰代; 郡山尚紀; 堀江一樹; 内田玲麻; 村松康和, 第162回日本獣医学会学術集会 飼育犬とその飼い主の口腔内細菌叢比較調査におけるMALDI-TOF MSシステムによる菌種同定法の有用性, 堀江一樹; 郡山尚紀; 西川泰代; 内田玲麻; 村松康和, 第162回日本獣医学会学術集会 北海道のマダニにおける吸血源とマダニ媒介性ウイルスの調査, 小柳優奈; 内田玲麻; 村松康和, 第162回日本獣医学会学術集会 飼い主とその飼育犬に関する口腔内細菌叢の相互関連性調査, 西川泰代; 郡山尚紀; 堀江一樹; 内田玲麻; 村松康和, 令和元年度北海道地区学会 ヒト由来口腔内細菌叢との比較に基づく犬由来口腔内細菌叢調査におけるMALDI-TOF MSシステムを用いた菌種同定法の意義, 堀江一樹; 郡山尚紀; 西川泰代; 内田玲麻; 村松康和, 令和元年度北海道地区学会 MALDI Biotyperを用いた健康牛の乳汁中における細菌の解析, 惠之巍; 村田亮; 大塚浩通; 内田玲麻; 村松康和, 第8回家畜感染症学会 豚由来Pasteurella multocida分離菌株における分子疫学調査, 村松康和; Tran Thi Nhat; 佐藤百合菜; 惠之巍; 内田玲麻, 平成30年度北海道地区学会 オホーツク管内で捕獲されたエゾシカ口腔内からの網羅的な細菌検出, 内海 博貴; 折原 詳; 内田 玲麻; 黒澤 拓也; 横山 敦志; 村松 康和, 第160回 日本獣医学会学術集会 と畜場における豚の肺膿瘍に関する分子疫学調査, 中山 大輔; 内田 玲麻; 黒澤 拓也; 井上 陽介; 山 奈津子; Thi Nhat Tran; 村松 康和 Single nucleotide polymorphisms解析に基づくウシ胎盤由来Coxiella burnetii DNAの遺伝子型別, 村松 康和, 第159回日本獣医学会学術集会 Multiple-locus variable-number tandem repeat analysisに基づくウシ胎盤から検出したCoxiella burnetii DNAの遺伝子型別, 村松 康和, 日本獣医師会北海道地区学会
Misc
Comparing Educational Effects for Veterinary Students Between Virtual Reality-Based and On-Site Based Practices on Learning of Meat Inspection for Cattle, 松山亮太; 内田玲麻; 千里今日子; 浮田真琴; 福田昭; 権平智; 福森理加; 臼井優; 樋口豪紀; 及川伸; 村松康和; 蒔田浩平, 獣医疫学雑誌, 28, 2024 北海道産ヤマトヤブカにおけるジカウイルス感受性の評価, 川江詩乃; 岩本夏奈; 江下優樹; 大場靖子; 澤洋文; 村松康和; 内田玲麻, 日本獣医学会学術集会講演要旨集, 164th (CD-ROM), 2021 ヤマトヤブカ体内におけるジカウイルスのQuasispecies評価, 三木要; 岩本夏奈; 江下優樹; 大場靖子; 澤洋文; 村松康和; 内田玲麻, 日本獣医学会学術集会講演要旨集, 164th (CD-ROM), 2021 飼い主とその飼育犬に関する口腔内細菌叢の相互関連性調査, 西川泰代; 郡山尚紀; 堀江一樹; 内田玲麻; 村松康和, 北海道獣医師会雑誌, 63, 2019 ヒト由来口腔内細菌叢との比較に基づく犬由来口腔内細菌叢調査におけるMALDI-TOF MSシステムを用いた菌種同定法の意義, 堀江一樹; 郡山尚紀; 西川泰代; 内田玲麻; 村松康和, 北海道獣医師会雑誌, 63, 2019 飼育犬とその飼い主の口腔内細菌叢比較調査におけるMALDI-TOF MSシステムによる菌種同定法の有用性, 堀江一樹; 郡山尚紀; 西川泰代; 内田玲麻; 村松康和, 日本獣医学会学術集会講演要旨集, 162nd, 2019 セラピードッグとその飼い主における口腔内細菌叢の比較, 西川泰代; 郡山尚紀; 堀江一樹; 内田玲麻; 村松康和, 日本獣医学会学術集会講演要旨集, 162nd, 2019 Q & A 公衆衛生編, Yasukazu MURAMATSU, Journal of Japan Veterinary Medical association, 71, (3) 129 - 131, 2018 03 MALDI-TOF質量分析システムによる犬口腔内からのBergeyella zoohelcumの検出, 原谷那美; 内田玲麻; 郡山尚紀; 鈴木章夫; 堀内基広; 村松康和, 北海道獣医師会雑誌, 59, 2015 イヌ口腔内からのBergeyella zoohelcumの分離, 村松康和; 原谷那美; 内田玲麻; 郡山尚紀; 鈴木章夫; 堀内基広, 日本獣医学会学術集会講演要旨集, 158th, 2015 C-3 下北半島に生息するニホンザル（Macaca fuscata）の寄生虫症および感染症に関する疫学調査, 浅川 満彦; 萩原 克郎; 村松 康和; 岡本 実; 渡辺 洋子; 三觜 慶, 霊長類研究所年報, 44, (44) 102 - 103, 2014 12 04 C-4 下北半島に生息するニホンザル (Macaca fuscata) の寄生虫症および感染症に関する疫学調査, 浅川 満彦; 渡辺 洋子; 萩原 克郎; 村松 康和; 岡本 実; 後藤 俊二, 霊長類研究所年報, (43) 112 - 112, 2013 11 北海道の飼育犬におけるCapnocytophaga属菌の侵淫調査, HATANAKA YUI; TAKIGUCHI MITSUYOSHI; GOTO MASAMITSU; MURAMATSU YASUKAZU, 日本獣医学会学術集会講演要旨集, 154th, 2012 08 31 高速相同性検索ソフトUSEARCHを用いた乳牛糞便DNA-Illumina分析データのメタゲノム解析, 遠藤大二; 北市みのり; 村松康和; 杉村逸郎; 林正信, 日本獣医学会学術集会講演要旨集, 153rd, 2012 鳥類の感染・寄生虫症モニタリング調査に基盤を置く保全医学研究 : 私立大学戦略的研究基盤形成支援事業2008年度～2010年度の概要, 浅川 満彦; 遠藤 大二; 萩原 克郎; 村松 康和; 寺岡 宏樹; 松田 一哉; 岡本 実; 谷山 弘行; 田村 豊; 横田 博, 鳥類臨床研究会報, (14) 25 - 28, 2011 12 Antimicrobial Susceptibility of Escherichia coli and Klebsiella spp. Isolated from Clinical Bovine Mastitis in the Ishikari Area of Hokkaido, SAKEMI Yoko; OKAKOI Masaaki; SHINODA Kojiro; MURAMATSU Yasukazu; UENO Hiroshi; TAMURA Yutaka, Journal of the Japan Veterinary Medical Association, 63, (3) 215 - 218, 2010 03 20 , 乳房炎由来細菌の薬剤感受性状況と、薬剤耐性化による公衆衛生上のリスクを評価する目的で、平成18年6月〜9月に北海道石狩地域で発症した牛乳房炎由来Escherichia coliおよび、Klebsiella属菌について薬剤感受性試験を行った。抗菌剤は、動物用および医療用の18種類を供試した。供試した106株のE. coli中29.2％、34株のKlebsiella属菌中91.2％に耐性株を認めたが、E. coliは家畜由来指標細菌の耐性率より低く、E. coli、Klebsiella属菌ともに二次選択薬である動物用抗菌剤に対してすべて感受性であった。したがって、該当地域における牛乳房炎由来E. coliおよびKlebsiella 属菌の人の健康への直接的な影響は少ないと考えられた。 動物病院におけるメチシリン耐性黄色ブドウ球菌の伝播, 田村豊; 村松康和; 中島千絵; 柳沢千恵; 鈴木定彦; 花木秀明, 感染症学雑誌, 82, 2008 日本の種雄牛におけるBSE感受性に関するプリオン遺伝子多型の出現頻度, 村松康和; 高橋健一; 早川宏之; 堀内基広; 上野弘志; 田村豊, 日本獣医学会学術集会講演要旨集, 144th, 2007 北海道のウシにおけるプリオン遺伝子の多形性, 村松康和; 堀内基広; 田村豊, 日本獣医学会学術集会講演要旨集, 141st, 2006 ベトナムの酪農牛におけるプリオン遺伝子の多形性, 村松康和; 要田正治; 小河孝; 堀内基広; 田村豊, 日本獣医学会学術集会講演要旨集, 141st, 2006 モンゴル在来ヒツジからのピロプラズマ目原虫DNAの検出, 村松康和; 八幡陽介; 堀内基広; 石黒直隆; 品川森一; 森田千春; 田村豊, 日本獣医学会学術集会講演要旨集, 140th, 2005 Ehrlichia ruminantium, Sudan [3], Yasukazu Muramatsu; Shin-Ya Ukegawa; Abdel Rahim Mohamed El Hussein; Magdi Badawi Abdel Rahman; Khalil Mohamed Ali Abdel Gabbar; Agnes Mumbi Chitambo; Tomoyoshi Komiya; Enala Tembo Mwase; Chiharu Morita; Yutaka Tamura, Emerging Infectious Diseases, 11, (11) 1792 - 1793, 2005 , 10.3201/eid1111.050744 A Preliminary Report of Parasitological and Microbiological survey of Freeranging Japanese Macaques (Macaca fuscata (Blyth)) in Boso Peninsula, Japan, SATOYOSHI Ayako; KABAYA Hajime; HAGIWARA Ko; TANIYAMA Hiroyuki; YOSHIZAWA Kazunori; TUJI Masayoshi; HAGIWARA Kasturo; MURAMATSU Yasukazu; ASAKAWA Mitsuhiko, Japanese Journal of Zoo and Wildlife Medicine, 9, (2) 79 - 83, 2004 09 01 , 10.5686/jjzwm.9.79, Because alien macaques (probably, Macaca mulatta) occur in the southern part of the Boso Peninsula, Japan, there is a possibility that their parasites and/or other pathogens may infect Japanese macaques (M.fuscata). To verify the possibility, this study was conducted mainly on parasites that included protozoan, helminth and arthropod, and other pathogens including Rickettsia and the Borna virus of 50 free ranging Japanese macaques in the peninsula. Consequently, 5 arthropod and nematode parasite species common to the Japanese macaque, namely Pedicinus obtusus, P.eurygaster, Streptopharagus pigmentatus, Strongyloides fulleborni, and Trichuris trichiura, were obtained in this survey. It is the first record of sites where Pedicinus spp. are seen in a host species.Coccidia and Babesia sp. were not obtained. Coxciella and the Borna virus from the same host samples will be published elsewhere. Detection of antibodies against spotted fever group Rickettsia (SFGR), typhus group Rickettsia (TGR), and Coxiella burnetii in human febrile patients in the Philippines, Gerry Amor Camer; Marissa Alejandria; Miguel Amor; Hiroshi Satoh; Yasukazu Muramatsu; Hiroshi Ueno; Chiharu Morita, Japanese Journal of Infectious Diseases, 56, (1) 26 - 28, 2003 02 新生子マウスの腸粘膜上皮細胞へのプリオン伝達の試み, 岡本実; 古岡秀文; 堀内基弘; 野口敬紀; 萩原克郎; 村松康和; 辻正義; 朝長啓造; 生田和良, 日本獣医学会学術集会講演要旨集, 136th, 2003 大阪における野生シカの紅斑熱群リケッチアに対する血清抗体調査及び遺伝子的検出, 岡林 環樹; 石塚 譲; 大谷 新太郎; 川井 裕史; 山田 倫章; 佐藤 弘; 村松 康和; 上野 弘志; 森田 千春, 日本獣医学会学術集会講演要旨集, 127回, 1999 03 An example of Ehrlichia disease in dog., 津山慎吾; 山内秀夫; 石坂智路; 久末正晴; 鄭光一; 村松康和; 森田千春; 長谷川篤彦; 辻本元, 日本獣医学会学術集会講演要旨集, 126th, 1998 08 Seroepidemiological Study of Coxiella burnetii in Cervus nippon in Northern Japan, YASUMOTO Morihiro; YANASE Tsuyoshi; MURAMATSU Yasukazu; UENO Hiroshi; MORITA Chiharu, Japanese Journal of Zoo and Wildlife Medicine, 2, (2) 101 - 106, 1997 09 01 , Q-fever, caused by Coxiella(C) burnetii, is one of the most important zoonoses. In the present study, the antibody against C.burnetii was analyzed by indirect immunofluorescence antibody(IFA) test in 168 individuals of Japanese deer (Cervus nippon) to determine the prevalence of Q-fever in wild deer of northern Japan. Specific antibody against C.burnetii was found in 72(42.9%) deer, including 24 of 46(52.2%) in Hokkaido and 48 of 122(39.3%) in Tohoku. In Toya and Shari, the positive rate between adult(66.7%) and immature animals(17.6%) was significant (p<0.01). In adult deer, a significant difference was also noted between Hokkaido and Tohoku(p<0.01). SURVEY OF NATURAL SCRAPIE IN JAPAN - ANALYSIS OF RFLP TYPES OF THE PRP GENE AND DETECTION OF PRPSC MAINLY IN SUFFOLK SHEEP, A ONODERA; T IKEDA; M HORIUCHI; N ISHIGURO; M ONUMA; N HIRANO; T MIKAMI; E HONDA; K HIRAI; K KAI; H YUGI; Y MURAMATSU; M SHINAGAWA, JOURNAL OF VETERINARY MEDICAL SCIENCE, 56, (4) 627 - 632, 1994 08 , 10.1292/jvms.56.627 FAMILIAL SCRAPIE CASES WITH SHORTENED INCUBATION PERIODS, M SHINAGAWA; Y MURAMATSU; A ONODERA; T MATSUI; M HORIUCHI; N ISHIGURO; M NAKAGAWA, JOURNAL OF VETERINARY MEDICAL SCIENCE, 55, (4) 665 - 667, 1993 08 , 10.1292/jvms.55.665
Awards & Honors
2019 09 , 北海道獣医師会, 日本獣医公衆衛生学会（北海道）奨励賞, 飼い主とその飼育犬に関する口腔内細菌叢の相互関連性調査 2008 09 , 北海道獣医師会, 平成19年度 北海道獣医師会 会長賞
Research Grants & Projects
Ministry of Health, Labour and Welfare, 厚生労働科学研究費補助金（食品の安全確保推進研究事業）, Pilot study conducible to build a support model for meat inspection by the diagnostic imaging based on the artificial intelligence technology, 202504, 202803 MEXT, Japan, Grant-in-Aid for Scientific Research, アニマルセラピーにおける動物由来感染症起因細菌の網羅的検索, 201704, 202003, Yasukazu MURAMATSU Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, The research for ansector of methicillin-resistant Staphylococcus pseudintermedius among dogs in Japan, 20140401, 20180331, Ishihara Kanako; TAMURA Yutaka; MURAMATSU Yasukazu, The recent appearance of methicillin-resistant Staphylococcus pseudintermedius (MRSP) is a concern for both veterinary and human healthcare. The current study compared the molecular characteristics of 43 MRSP isolates from dogs in Japan with those of MRSP from previous reports using multilocus sequence typing, SCCmec typing, and detection of antimicrobial resistance genes.
Three related clonal lineages, ST71, ST169, and the newly registered ST354, were observed amongst SCCmec II-III isolates from Japan, despite MRSP SCCmec II-III isolates being thought to belong to a single clonal lineage. The majority of SCCmec II-III isolates belonging to ST169 (9/11) and ST354 (3/3), but not ST71 (0/11), harboured tetM. Since the allele sequences of two loci (ack and sar) differ between ST71 and ST169 isolates, ST71 and ST169 clones have been derived from a common ancestral clone. ST354 is likely a variant clone of ST169. 科学技術振興機構, 「食の安全」高度化を可能にする細菌叢プロファイリング法の開発と応用, 2008, 2008, 村松 康和, 主な食品媒介性病原細菌群の簡易、高感度かつ高特異的な検査系を確立し、「食の安全」高度化に貢献する。特許技術に基づ食品媒介性病原細菌群DNA検出プライマー最適化を行い、高感度・高特異的な細菌叢DNAプロファイリングにより、集約化・大規模化が急速に進む北海道酪農における、信頼度の高い「食の安全」の為の牛群管理高度化を目指す。 Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Epidemiological survey on the high-risk zoonosis in the southeast asian countries, 2005, 2007, TAMURA Yutaka; MURAMATSU Yasukazu, This study investigates to clarify the present situation of high-risk zoonosis to invade our country through an animal and food in the southeast asian countries.
1. Rabies virus
(1) Indonesia : The N gene was detected from a dog brain that suffered rabies, and resembled that of isolates in China and Philippines.
(2) Vietnam : The N gene was not detected from bat brain, but neutralizing antibody for rabies virus was found in dog serum.
2. Q fever rickettsia
(1) Thailand : An antibody for Q fever was detected from chicken and cattle serum, and detected Coxiella DNA from ticks attached to cattle.
(2) Indonesia : An antibody for Q fever was detected from chicken and cattle serum.
(3) Vietnam : An antibody for Q fever was detected from chicken and cattle serum, and Coxiella DNA was detected from chicken and cattle.
3. Drug resistant bacteria
(1) Thailand : A lot of multiple drug-resistant Enterococci were isolated from chicken feces.
(2) Indonesia: Multiple drug-resistant Enterococci was isolated from chicken feces, and a lot of vancomycin resistant Enterococci (VRE) were isolated.
(3) Vietnam : A lot of multiple drug-resistant Enterococci were isolated from chicken and cattle feces.
From these results, rabies virus, Coxiella burnetti and drug resistant bacteria including VRE spread in the southeast asian countries which surrounded our country, and the possibility exists to invade our country through an animal and food. Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Experimental transmission of abnormal prion protein (PrPsc) in the small intestinal epithelial cells of neonatal mice, 2003, 2004, TANIYAMA Hiroyuki; HAGIWARA Katsurou; MURAMATSU Yasukazu, Using an immunohistochemical method, we attempted to detect the transmission of abnormal prion protein (PrPsc) to the enterocytes of the small intestine of neonatal mice by oral exposure with sheep brain affected by scrapie. Five 1-day-old neonatal mice were exposed by oral inoculation to the homogenized brain of a scrapie-affected sheep. In the small intestine of all mice 1 hour after inoculation, immunoreactivity with antinormal prion protein (PrPc) antibody was seen in the cytoplasm of viilus enterocytes. This finding suggests transmission of abnormal PrPsc into the cytoplasm of enterocytes. In control mice treated with normal sheep brain, no PrPc signal was seen in enterocytes of the small intestine. Immunopositivity for neurofilament protein and glial fibrillary acidic protein was seen in the cytoplasm of enterocytes of mice inoculated with scrapie and normal sheep brain. This suggests that the enterocytes of neonatal mice can absorb PrPsc and other macromolecular proteins of the sheep brain affected by scrapie and may be more important than previously thought as a pathway for PrPsc transmission in neonatal animals. After 7 days to 3months of oral inoculation to the homogenized brain of a scrapie-affected sheep, immunoreactivity with antinormal prion protein (PrPc) antibody was not seen in the cytoplasm of villus enterocytes and other tissues in the mucosa. The results suggest that the term of the transmission of abnormal priors protein (PrPsc) ineeds over 3 months and another system of the transmission is present in the mucosa of mouse. Lam inine reseptor (LR) and normal prion were more frequently present in the cytoplasm of enterocytes of suckling mice and young cattle than adult mice and cattle, thus young animals have the higher risk of transmission of abnormal prion than adult animals, via the gastrointestinal menbrane. Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Molecular phylogenic study of Ehrlichia spp. microorganisms as the etiological agents of emerging diseases in Japan, 2002, 2004, MURAMATSU Yasukazu, 1)Ehrlichial gene was detected from small rodents trapped in a Lyme disease-endemic area in Hokkaido, the northernmost island of Japan. Primer pairs of 168 rDNA targeting the genus of Ehrlichia and other region of the 16S rDNA specific for E. chaffeensis and E. muris were used for identification. The DNA fragment specific for 16S rDNA of E. spp. was detected from 4 of 94 Apodemus. speciosus mice (positive rate 4.3%) and 5 of 73 Clethrionomys rufocanus bedfordiae mice (positive rate 6.8%). The nucleotide sequence of the amplified 168 rDNA fragment was most similar to those of E muris-like ehrlichia, E spp HF565 and Shizuoka-36 originating in the northeastern part of Japan. In phylogenic analysis based on 16S rDNA sequences, these northeastern and southwestern groups of E. murrslike ehrlichia made a cluster with E. muris group microorganisms. These results suggest that there are two groups of E. murislike microorganisms in Japan.
2)A novel genotype of Ehrlichia rummantium, the causative agent of heartwater, was genetically detected in the Sudan. The new genotype was provisionally named as "Gedaref". The results of phylogenic analyses on the regions of pCS20 and major antigen protein I (map)) genes revealed that the ehrlichia) strain belongs to E. ruminantium-group, but does not correspond with any known strains of E. ruminantium. Furthermore, as the results of phylogenic analyses on map), "Gedaref" made clusters with several strains of E. ruminantium other than Um Banein, which had been known as the sole strain of E. ruminantium in the Sudan. This work provided the evidence that not only Um Banein strain but also the other strain exist in the Sudan. Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Etiological and molecular genetic surveillance of scrapie in Mongolia, 2000, 2002, HORIUCHI Motohiro; MURAMATSU Yasukazu; ISHIGURO Naotaka, Mongolia has a far long history for sheep-farming and is now farming over twelve million sheep. In the future, Mongolia has a potential for the major provider of livestock products among the world. Thus surveillance of prion diseases in animal in Mongolia will provide important information to evaluate the safety of livestock products in the country. During the research term, we visit to central, west, and east part of Mongolia, we did not get a positive indication on the existence of scrapie-suspected sheep in hearing. We also checked PrPSc in the medulla oblongata from sheep showed neurological symptoms, but all were negative for PrPSc.
In order to investigate the diversity of PrP gene of sheep in Mongolia, we collected a total of 470 venous blood samples from 9 different breeds (Khalkh, Yeroo, Orkhon, Khangai, Sartuul, Govi-altai, Bayad, Darhad, and Symber-Karakul) and purified genomic DNA. PrP genotype (amino acid type) was determined by direct sequencing of PCR-amplified fragment corresponding to PrP open reading frame. With regard to PrP genotype at codon 171 determining susceptibility to scrapie, sheep carrying 171-Gln/Gln genotype that is associated with the susceptibility to scrape, varied from 58 to 97% among the breeds. Sheep carrying 171-Arg/Arg or 171-Arg/Gln genotype that confers resistance to scrapie infection were less abundant, especially only a small percentage of sheep possessed homozygous for 171-Arg alle. These results indicate sheep in Mongolia is composed rather of scrapie-susceptible population, so continuous surveillance as well as preventive measures against prion-infected animal are important to evaluate the risk of prion disease. These results are also useful for performing a selective breeding program that produce scrapie-resistant flock. Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Investigation of Listeria monocytogenes in organic farming environments based on detection of VBNC cells of the organism., 1998, 2000, UBNO Hiroshi, Listeria monocytogenes has a possibility to form VBNC (Viable But Non-Culturable) cells in natural environments, although knowledge of this phenomenon in the bacteria is very limitted. The author found that the suitable conditions for detecting VBNC cells of a test strain of L.monocytogenes were incubation at a concentration of 1/2 to 2/3 MIC in brain heart infusion for 4 to 8 hours after contact with levofloxacin. Because these conditions did not allow division of the test organism, but allowed formation of elongated VBNC cells, stained by Giemsa solution, in a liquid medium.
About 10^7/ml of viable test organisms were added to sterilized soil solution, PBS and distilled water, and settled for 56 days at 0℃. During this period, plate counts and total bacterial counts were almost constant in sterilized soil solution, whereas VBNC cells were frequently (43% of numbers of total cells) found in 7 days after settlement. The similar results were obtained in PBS, although VBNC cells were seldom observed in distilled water. These results suggest that entry of L.monocytogenes organisms into VBNC state occurs at cold and starved conditions.
After inoculating 6×10^7/ml of viable organisms in sterilized soil solution, the solution was concentrated by ten using PBS.Plate counts increased to 4.7×10^8/ml in the solution and almost same numbers (2.1×10^8/ml) of total bacterial counts were observed by an enzyme-linked immuno-sorbent assay (EIA) using peroxydase labeled L.monocytogenes antibodies. On the other hand, there was no stained organism in a sample taken from un-sterilized soil solution that yielded a plate count of 9.2×10^5/ml. These results suggest that L.monocytogenes in soil could be detected specifically by using the EIA. 日本学術振興会, 科学研究費助成事業, リポ多糖解析に基づくcoxiella burnetiiの分子疫学的研究, 1997, 1998, 村松 康和, 北海道のウシの生乳から、マウスを用いて継代・分離した2株のCoxiella burnetii、Nine Mile II相菌(American Type Culture Collectionより購入;ATCC VR-616)、Califonia株(同;ATCC VR-614)、およびPricilla株(岐阜大学;平井克哉教授より分与)を発育鶏卵卵黄嚢内接種により大量培養した。次いでWilliams,J.C.et al.(1981)の方法に従いRenografinの密度勾配を用いた遠心分離によりC.burnetiiを精製した。
得られたコクシエラの精製菌を用いてSDS-PAGEを行った。次いで銀染色、およびWesternblot analysis(WBA)により、本研究による分離菌と、既存の各菌株との蛋白、および抗原性状に関する比較を行った。
銀染色の結果、本研究で得られた分離株には、比較に用いたNine Mile H相菌株、およびCalifornia株と異なる、株特異な蛋白画分は認められなかった。また、用いた全てのコクシエラで全蛋白の泳動像に差異は認められなかった。これらの結果は、国内外における以前の報告を裏付けるものである。
抗コクシエラ・マウス血清を用いたWBAの結果、分離2株中、1株において、他のコクシエラとは異なる抗原性状が認められた。現在、糖鎖の性状について検討中である。
血液吸着濾紙により採取した見かけ上健康なネコの乾燥濾紙血液を用いて、間接蛍光抗体法、およびPCRを行った。その結果、今回初めてPCRにより乾燥濾紙血液からのコクシエラ、およびネコひっかき病の主要な原因リケッチアであるBartonella henselaeに特異なDNAの検出に成功した。この事は乾燥濾紙血液サンプルが広範囲にわたるコクシエラの疫学調査に有用であることを示している(現在投稿準備中)。 日本学術振興会, 科学研究費助成事業, PCR-ELISAによるQ熱の診断に関する研究, 1995, 1995, 村松 康和, BGM細胞に0.001〜0.1%濃度の界面活性剤Triton X-100 (TX100)を加え、鏡検により細胞の破砕を観察した。Coxiella burnetii Nine Mile II相菌(ATCC VR-616)の培養上清(細胞外の菌体が主体)、及び感染BGM細胞(細胞内の菌体が主体)を別々に回収した。
培養上清について各々0.001〜0.01%濃度のTX100を加えた試料と無添加の試料を遠心後、PCRを行った結果、無添加の場合と検出感度に差は無かった。
感染細胞では0.01、0.02%のTX100を加え遠心した試料、免疫鉄ビーズで菌体を回収した(この方法についてはVeterinary microbiologyに掲載決定)。試料、及びTX100添加後、免疫鉄ビーズで菌体を回収した試料でPCRを行った。0.01%濃度のTX100を加えた試料では、TX100無添加で免疫鉄ビーズにより菌体回収した試料と比べPCRの結果、検出感度の上昇が見られた。また、0.01%TX100は免疫鉄ビーズと菌体の反応を阻害しないことが判った。牛乳の場合、0.01%TX100のみ添加では遠心後に大量の堅固なpelletが残り、PCRサンプル調整の妨げになると共に検出感度が低下した。牛乳からのPCR-ELISAサンプル調整では0.01%TX100添加+免疫鉄ビーズによる菌体回収で検出感度が良かった。
ウシ60頭の生乳から、この方法によりサンプル調整し、PCR-ELISAによりC. burnetii検出を行った。PCR-ELISAは先に提出した申請書に記載した方法に準じて行った。10頭が陽性で(陽性率16.7%)、うち5頭が強陽性であった。PCR-ELISAは高感度に、多数の試料の判定が出来る利点を持つ。(以上の結果について第121回日本獣医学会で発表予定。Applied and environmental microbiologyに投稿準備中。)
社会貢献活動情報
Social Contribution
2025 11 13 - 2025 11 13 , 人工知能（AI）を活用した 食肉検査について, 千葉県, 千葉県食肉技術研修会# 2025 11 01 - 2025 11 01 , ヒトに感染する注意すべきペットの病原性口腔内細菌 ～Bergeyella zoohelcumを中心に～, 北海道獣医師会、北海道歯科医師会, One Health連携シンポジウム# 2019 09 30 - 2019 09 30 , 人の生活に身近な野生動物、伴侶動物と共生する微生物, （一社）札幌市小動物獣医師会#
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Last Update :2026/09/14
Researcher Profile and Settings
Name
| MURAMATSU Yasukazu |
Affiliation (Master)
| School of Veterinary Medicine, Department of Veterinary Medicine |
Association Memberships
| JAPANESE SOCIETY FOR BACTERIOLOGY |
| THE JAPANESE ASSOCIATION FOR INFECTIOUS DISEASES |
| 人と動物の共通感染症研究会 |
| THE JAPANESE SOCIETY FOR VIROLOGY |
| JAPAN VETERINARY MEDICAL ASSOCIATION |
| THE JAPANESE SOCIETY OF VETERINARY SCIENCE |
Research Activities
Research Areas
| Life sciences, Veterinary medicine |
Published Papers
| The Evaluation of Zika virus susceptibility in Aedes (Ochlerotatus) japonicus from Hokkaido, Japan., Leo Uchida; Kana Iwamoto; Shino Kawae; Makoto Shimooka; Ronald Enrique Morales-Vargas; Yuki Eshita; Yasuko Orba; Hirofumi Sawa; Yasukazu Muramatsu, Japanese journal of infectious diseases, 2026 04 30 |
| Detection of haemosporidian parasite lineages from wild bird carcasses collected in eastern Japan., Momoko Eki; Mizuki Sasaki; Yasukazu Muramatsu; Mari Kobayashi; Takanori Ishikawa; Leo Uchida, The Journal of veterinary medical science, 87, (7) 838 - 842, 2025 07 11 |
| Structure and milk hygiene of dairy cooperative value chains in an intensive production area of Uganda—A bottleneck of intervention, Yoshiharu Sugino; James Bugeza; David Bahame; Joseph Byaruhanga; Haruka Shimazaki; Masahiko Anzai; Taishi Kayano; William Mwebembezi; Andrew Akashaba; Taku Shimada; Yasukazu Muramatsu; Kohei Makita, Frontiers in Sustainable Food Systems, 7, 2023 |
| Identification of three novel genes in Phenuiviridae detected from Aedes mosquitoes in Hokkaido, Japan., Leo Uchida; Yoshimi Sakurai; Makoto Shimooka; Ronald Enrique Morales-Vargas; Katsuro Hagiwara; Yasukazu Muramatsu, Japanese journal of infectious diseases, 76, (1) 55 - 63, 2022 09 30 |
| 3種のヤブカ体内において選択されたジカウイルスのゲノム変異評価, 下岡 誠; 三木 要; 江下 優樹; 大場 靖子; 澤 洋文; 村松 康和; 内田 玲麻, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 165回, [F1P - 06], 2022 09 |
| Cryptosporidium sp. Mrb001 detected from Myodes rufocanus bedfordiae, an indigenous vole of Hokkaido, Japan, Yasukazu Muramatsu; Leo Uchida; Yutaka Tamura; Mitsuhiko Asakawa, J. Rakuno Gakuen Univ., 2022 03 |
| Isolation and Characterization of Mosquito-Associated Spiroplasma cantharicola from Aedes japonicus Collected in Hokkaido, Japan., Makoto Shimooka; Yoshimi Sakurai; Yasukazu Muramatsu; Leo Uchida, Insects, 12, (12) , 2021 11 25 |
| 飼い主と飼育犬における口腔内細菌の検出頻度と細菌叢の比較, 村松 康和; 井谷 寛; 野村 美月; 内田 玲麻; 郡山 尚紀, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 164回, [FO - 53], 2021 09 |
| Zika Virus Potential Vectors among Aedes Mosquitoes from Hokkaido, Northern Japan: Implications for Potential Emergence of Zika Disease., Leo Uchida; Miki Shibuya; Ronald Enrique Morales-Vargas; Katsuro Hagiwara; Yasukazu Muramatsu, Pathogens (Basel, Switzerland), 10, (8) , 2021 07 24 |
| Effect of chemical tick control practices on tick infestation and Theileria parva infection in an intensive dairy production region of Uganda., Miyama, T; Byaruhanga J; Okamura, I; Uchida, L; Muramatsu, Y; Mwebembezi, W; Vudriko, P; Makita, K, Ticks Tick Borne Dis., Elsevier BV, 11, (4) 101438 - 101438, 2020 07 |
| Prevalence of sub-clinical mastitis and its association with milking practices in an intensive dairy production region of Uganda., Miyama, T; Byaruhanga J; Okamura, I; Nagahata, H; Murata, R; Mwebembezi, W; Muramatsu, Y; Makita, K, J Vet Med Sci, Japanese Society of Veterinary Science, 82, (4) 488 - 493, 2020 04 |
| FTA-Sodium hydroxide-based polymerase chain reaction (PCR): An efficient and cheaper option for Theileria parva detection in dairy cattle in Mbarara, Uganda., Leo Uchida; Joseph Byaruhanga; Ikuo Okamura; Takeshi Miyama; Yasukazu Muramatsu; Patrick Vudriko; Kohei Makita, The Journal of veterinary medical science, 82, (2) 188 - 192, 2020 02 18 |
| PCR PRIMER DESIGN METHOD FOR DIFFERENTIATING AMONG SALMONELLA SEROGROUPS BASED ON AN ALGORITHM TARGETING GENE-FLANKING REGIONS, Yasukazu MURAMATSU, The Southeast Asian Journal of Tropical Medicine and Public Health, 50, (5) , 2019 09 |
| Bergeyella zoohelcum isolated from oral cavities of therapy dogs, Yasukazu MURAMATSU; Nami HARAYA; Kazuki HORIE; Leo UCHIDA; Takanori KOORIYAMA; Akio SUZUKI; Motohiro HORIUCHI, Zoonoses and Public Health, 2019 08 |
| MALDI Biotyperを用いた豚の肺膿瘍における網羅的細菌解析(Comprehensive identification of bacteria in pig lung abscesses using the MALDI Biotyper system), 内田 玲麻; 中山 大輔; 黒澤 拓也; Tran Nhat T.; 村田 亮; 宮庄 拓; 山 奈津子; 村松 康和, 酪農学園大学紀要(自然科学編), 酪農学園大学, 43, (2) 85 - 96, 2019 03 |
| MALDI Biotyperを用いた健康牛の乳汁中における細菌の解析, 惠 之巍; 村田 亮; 大塚 浩通; 内田 玲麻; 村松 康和, 家畜感染症学会誌, 家畜感染症学会誌編集委員会, 7, (4) 181 - 181, 2018 12 |
| Survey of tick-borne zoonotic viruses in wild deer in Hokkaido, Japan., Leo Uchida; Daisuke Hayasaka; Mya Myat Ngwe Tun; Kouichi Morita; Yasukazu Muramatsu; Katsuro Hagiwara, The Journal of veterinary medical science, 80, (6) 985 - 988, 2018 06 29 |
| Genotyping of Coxiella burnetii detected from bovine placenta based on Multiple-locus variable-number tandem repeat analysis, Yasukazu MURAMATSU, Research of One Health, 2016 12 |
| 液体クロマトグラフ質量分析計(LC/MS)を用いた節足動物媒介性ウイルスの探索, 内田 玲麻; 岡本 健太; 鍋島 武; 青木 康太郎; 村松 康和; 森田 公一; 長谷部 太, 北海道獣医師会雑誌, (公社)北海道獣医師会, 60, (8) 427 - 427, 2016 08 |
| Detection of methicillin-resistant Staphylococcus pseudintermedius ST169 and novel ST354 SCCmec II-III isolates related to the worldwide ST71 clone, K. Ishihara; A. Koizumi; M. Saito; Y. Muramatsu; Y. Tamura, Epidemiology and Infection, Cambridge University Press, 144, (2) 434 - 442, 2016 01 01 |
| Epidemiological Analysis of Methicillin-Resistant Staphylococcus aureus Carriage among Veterinary Staff of Companion Animals in Japan, Kanako Ishihara; Mieko Saito; Natsumi Shimokubo; Yasukazu Muramatsu; Shigeki Maetani; Yutaka Tamura, JOURNAL OF VETERINARY MEDICAL SCIENCE, 76, (12) 1627 - 1629, 2014 12 |
| SEROEPIDEMIOLOGIC SURVEY IN THAILAND OF COXIELLA BURNETII INFECTION IN CATTLE AND CHICKENS AND PRESENCE IN TICKS ATTACHED TO DAIRY CATTLE, Yasukazu Muramatsu; Noriyo Usaki; Chalermchaikit Thongchai; Indhira Kramomtong; Poonsuk Kriengsak; Yutaka Tamura, SOUTHEAST ASIAN JOURNAL OF TROPICAL MEDICINE AND PUBLIC HEALTH, 45, (5) 1167 - 1172, 2014 09 |
| Genetic Diversity in the Prion Protein Gene (PRNP) of Domestic Cattle and Water Buffaloes in Vietnam, Indonesia and Thailand, Leo Uchida; Agus Heriyanto; Chalermchaikit Thongchai; Tran Thi Hanh; Motohiro Horiuchi; Kanako Ishihara; Yutaka Tamura; Yasukazu Muramatsu, JOURNAL OF VETERINARY MEDICAL SCIENCE, 76, (7) 1001 - 1008, 2014 07 |
| Antimicrobial Susceptibility of Indicator Bacteria Isolated from Chickens in Southeast Asian Countries (Vietnam, Indonesia and Thailand), Masaru Usui; Shuhei Ozawa; Hiroyuki Onozato; Rikiya Kuge; Yuko Obata; Tomoko Uemae; Pham Thi Ngoc; Agus Heriyanto; Tongchai Chalemchaikit; Kohei Makita; Yasukazu Muramatsu; Yutaka Tamura, JOURNAL OF VETERINARY MEDICAL SCIENCE, 76, (5) 685 - 692, 2014 05 |
| Methicillin-resistant Staphylococcus aureus carriage among veterinary staff and dogs in private veterinary clinics in Hokkaido, Japan, Kanako Ishihara; Mieko Saito; Natsumi Shimokubo; Yasukazu Muramatsu; Shigeki Maetani; Yutaka Tamura, MICROBIOLOGY AND IMMUNOLOGY, 58, (3) 149 - 154, 2014 03 |
| Distribution of antimicrobial-resistant lactic acid bacteria in natural cheese in Japan, Kanako Ishihara; Kumiko Nakajima; Satoko Kishimoto; Fumiaki Atarashi; Yasukazu Muramatsu; Akitoyo Hotta; Satomi Ishii; Yasuyuki Takeda; Masanori Kikuchi; Yutaka Tamura, Microbiology and Immunology, 57, (10) 684 - 691, 2013 10 |
| Contribution of the AcrAB-TolC efflux pump to high-level fluoroquinolone resistance in Escherichia coli isolated from dogs and humans, Toyotaka Sato; Shin-Ichi Yokota; Torahiko Okubo; Kanako Ishihara; Hiroshi Ueno; Yasukazu Muramatsu; Nobuhiro Fujii; Yutaka Tamura, Journal of Veterinary Medical Science, 75, (4) 407 - 414, 2013 |
| Factors associated with antimicrobial-resistant Escherichia coli in zoo animals, Kanako Ishihara; Yuko Hosokawa; Kohei Makita; Jun Noda; Hiroshi Ueno; Yasukazu Muramatsu; Hiroshi Ueno; Takeshi Mukai; Hideaki Yamamoto; Masaki Ito; Yutaka Tamura, RESEARCH IN VETERINARY SCIENCE, 93, (2) 574 - 580, 2012 10 |
| Parasitic Nematodes from Albino Individuals of Japanese Rat snakes Elaphe climacophora in Yamaguchi Prefecture, Japan, MATSUDA, Kazuya; MURAMATSU, Yasukazu; ASAKAWA, Mitsuhiko, 獣医畜産新報, 文永堂出版, 65, (9) 753 - 756, 2012 09 |
| Effects of climatic elements on Campylobacter-contaminated chicken products in Japan., Ishihara K; Takahashi R; Andoh M; Makita K; Kamiji S; Ueno H; Muramatsu Y; Tamura Y, Epidemiol Infect, Cambridge University Press, 140, (6) 991 - 6, 2012 06 |
| 高速相同性検索ソフトUSEARCHを用いた乳牛糞便DNA-Illumina分析データのメタゲノム解析, 遠藤 大二; 北市 みのり; 村松 康和; 杉村 逸郎; 林 正信, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 153回, 238 - 238, 2012 03 |
| Seasonal Variation in Campylobacter-contaminated Retail Chicken Products: A Year-Round Investigation in Japan, Kanako Ishihara; Ryuta Takahashi; Masako Andoh; Hiroshi Ueno; Yasukazu Muramatsu; Yutaka Tamura, JOURNAL OF VETERINARY MEDICAL SCIENCE, 74, (1) 117 - 120, 2012 01 |
| Antimicrobial Susceptibility of Escherichia coli Isolates from Wild Mice in a Forest of a Natural Park in Hokkaido, Japan, Kanako Ishihara; Kyoko Kanamori; Tetsuo Asai; Akemi Kojima; Toshio Takahashi; Hiroshi Ueno; Yasukazu Muramatsu; Yutaka Tamura, JOURNAL OF VETERINARY MEDICAL SCIENCE, 73, (9) 1191 - 1193, 2011 09 |
| 鶏肉のカンピロバクター汚染に対する気候要素の影響, 石原 加奈子; 高橋 隆太; 安藤 匡子; 蒔田 浩平; 上野 弘志; 村松 康和; 田村 豊, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 152回, 260 - 260, 2011 08 |
| カンピロバクターによる畜産食品汚染の季節変動, 石原 加奈子; 高橋 隆太; 安藤 匡子; 上野 弘志; 村松 康和; 田村 豊, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 151回, 243 - 243, 2011 03 |
| Occurrence and Molecular Characteristics of Methicillin-Resistant Staphylococcus aureus and Methicillin-Resistant Staphylococcus pseudintermedius in an Academic Veterinary Hospital, Kanako Ishihara; Natsumi Shimokubo; Akie Sakagami; Hiroshi Ueno; Yasukazu Muramatsu; Tsuyoshi Kadosawa; Chie Yanagisawa; Hideaki Hanaki; Chie Nakajima; Yasuhiko Suzuki; Yutaka Tamura, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 76, (15) 5165 - 5174, 2010 08 |
| Availability of Portable Endotoxin Test System in Commercial Milk and Milk Drinks, SAKEMI Yoko; TAKAHASHI Junkichi; FUNATSUKI Kiyomi; ISHIHARA Kanako; UENO Hiroshi; MURAMATSU Yasukazu; TAMURA Yutaka, Journal of the Japan Veterinary Medical Association, Japan Veterinary Medical Association, 63, (2) 145 - 147, 2010 02 20 |
| 犬由来大腸菌のフルオロキノロン耐性機構, 佐藤 豊孝; 石原 加奈子; 村松 康和; 上野 弘志; 岡林 環樹; 横田 伸一; 藤井 暢弘; 田村 豊, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 146回, 319 - 319, 2008 09 |
| Frequencies of PRNP gene polymorphisms in Vietnamese dairy cattle for potential association with BSE, Y. Muramatsu; Y. Sakemi; M. Horiuchi; T. Ogawa; K. Suzuki; M. Kanameda; T. T. Hanh; Y. Tamura, Zoonoses and Public Health, 55, (5) 267 - 273, 2008 06 |
| 動物病院におけるメチシリン耐性黄色ブドウ球菌の検出, 坂上 亜希恵; 村松 康和; 上野 弘志; 廉澤 剛; 田村 豊, 日本獣医学会学術集会講演要旨集, (公社)日本獣医学会, 144回, 124 - 124, 2007 08 |
| Seroepidemiologic survey of Coxiella burnetii and attempt to detect Coxiella DNA in aged non-laying chickens in a prefecture of Japan where poultry farming prospers, Yasukazu Muramatsu; Kikuto Hukuta; Sayaka Satoh; Masatake Muramatsu; Masaaki Nishimura; Hajime Nagahata; Hiroshi Ueno; Chiharu Morita; Yutaka Tamura, JOURNAL OF VETERINARY MEDICAL SCIENCE, 68, (9) 1007 - 1008, 2006 09 |
| Organizing pneumonia associated with Q fever, ONOE Kazuyuki; NASUHARA Yasuyuki; KASAHARA Ikumi; HIZAWA Nobuyuki; NISHIMURA Masaharu; UENO Hiroshi; MURAMATSU Yasukazu; MORITA Chiharu; TAMURA Yutaka, Nihon Naika Gakkai Kaishi, The Japanese Society of Internal Medicine, 94, (5) 961 - 963, 2005 05 10 |
| Detection of Ehrlichial DNA in small rodents captured in a woodland area of Hokkaido, the northernmost island of Japan, where lyme disease is endemic, Yasukazu Muramatsu; Emiko Ikeda; Chiharu Morita; Yutaka Tamura, Japanese Journal of Infectious Diseases, 58, (5) 316 - 319, 2005 |
| Spotted fever group rickettsiae from ticks captured in Sudan, Chiharu Morita; Abdel Rahim Mohamed El Hussein; Etsurou Matsuda; Khalil Mohamed Ali Abdel Gabbar; Yasukazu Muramatsu; Magdi Badawi Abdel Rahman; Ali Mohamed Hassan Eleragi; Shawgi Mohamed Hassan; Agnes Mumbi Chitambo; Hiroshi Ueno, Japanese Journal of Infectious Diseases, 57, (3) 107 - 109, 2004 06 |
| Experimental transmission of abnormal prion protein (PrPsc) in the small intestinal epithelial cells of neonatal mice, M. Okamoto; H. Furuoka; M. Horiuchi; T. Noguchi; K. Hagiwara; Y. Muramatsu; K. Tomonaga; M. Tsuji; C. Ishihara; K. Ikuta; H. Taniyama, Veterinary Pathology, 40, (6) 723 - 727, 2003 11 |
| Characterization of spotted fever group rickettsiae detected in dogs and ticks in Okinawa, Japan, Hiroshi Satoh; Yurie Motoi; Gerry A. Camer; Hisashi Inokuma; Masako Izawa; Tsuyoshi Kiyuuna; Norichika Kumazawa; Yasukazu Muramatsu; Hiroshi Ueno; Chiharu Morita, Microbiology and Immunology, 46, (4) 257 - 263, 2002 01 01 |
| First detection of Ehrlichia platys in dogs and ticks in Okinawa, Japan, Yurie Motoi; Hiroshi Satoh; Hisashi Inokuma; Tsuyoshi Kiyuuna; Yasukazu Muramatsu; Hiroshi Ueno; Çhiharu Morita, Microbiology and Immunology, 45, (1) 89 - 91, 2001 01 01 |
| Seroprevalence of antibodies against spotted fever group Rickettsia among dogs and humans in Okinawa, Japan, Hiroshi Satoh; Akemi Tsuneki; Hisashi Inokuma; Norichika Kumazawa; Yoshihide Jahana; Tsuyoshi Kiyuuna; Tamaki Okabayashi; Yasukazu Muramatsu; Hiroshi Ueno; Chiharu Morita, Microbiology and Immunology, Center for Academic Publications Japan, 45, (1) 85 - 87, 2001 |
| Detection of serum antibodies to Bartonella henselae and Coxiella burnetii from Japanese children and pregnant women, Kei Numazaki; Hiroshi Ueno; Kazuko Yokoo; Yasukazu Muramatsu; Shunzo Chiba; Chiharu Morita, Microbes and Infection, Elsevier Masson SAS, 2, (12) 1431 - 1434, 2000 |
| Serosurvey of wild rodents for Rickettsioses (spotted fever, murine typhus and Q fever) in Java Island, Indonesia, Ima Nurisa Ibrahim; Tamaki Okabayashi; Ristiyanto; Enny Wahyu Lestari; Tsuyoshi Yanase; Yasukazu Muramatsu; Hiroshi Ueno; Chiharu Morita, European Journal of Epidemiology, 15, (1) 89 - 93, 1999 |
| Short report: Prevalence of antibodies against spotted fever, murine typhus, and Q fever rickettsiae in humans living in Zambia, Tamaki Okabayashi; Futoshi Hasebe; Kenny L. Samui; Aaron S. Mweene; Shanker G. Pandey; Tsuyoshi Yanase; Yasukazu Muramatsu; Hiroshi Ueno; Chiharu Morita, American Journal of Tropical Medicine and Hygiene, American Society of Tropical Medicine and Hygiene, 61, (1) 70 - 72, 1999 |
| Detection of Coxiella burnetii from dust in a barn housing dairy cattle, Tsuyoshi Yanase; Yasukazu Muramatsu; Iwao Inouye; Tamaki Okabayashi; Hiroshi Ueno; Chiharu Morita, Microbiology and Immunology, Blackwell Publishing Asia, 42, (1) 51 - 53, 1998 |
| Detection of coxiella burnetii in cow's milk by PCR-enzyme-linked immunosorbent assay combined with a novel sample preparation method, Yasukazu Muramatsu; Tsuyoshi Yanase; Tamaki Okabayashi; Hiroshi Ueno; Chiharu Morita, Applied and Environmental Microbiology, 63, (6) 2142 - 2146, 1997 06 |
| Seasonal variations in the presence of antibodies against Coxiella burnetii in dairy cattle in Hokkaido, Japan, Tsuyoshi Yanase; Yasukazu Muramatsu; Hiroshi Ueno; Chiharu Morita, Microbiology and Immunology, Blackwell Publishing Asia, 41, (2) 73 - 75, 1997 |
| Seroepidemiological survey of lymphocytic choriomeningitis virus in wild house mice in China with particular reference to their subspecies, Chiharu Morita; Kimisachi Tsuchiya; Hiroshi Ueno; Yasukazu Muramatsu; Akiko Kojimahara; Hitoshi Suzuki; Nobumoto Miyashita; Kazuo Moriwaki; Mei-Lei Jin; Xiang-Lin Wu; Feng-Shan Wang, Microbiology and Immunology, Blackwell Publishing Asia, 40, (4) 313 - 315, 1996 |
| Serological survey of spotted fever group rickettsia in wild rats in thailand in the 1970s, Tamaki Okabayashi; Kimiyuki Tsutiya; Yasukazu Muramatsu; Hiroshi Ueno; Chiharu Morita, Microbiology and Immunology, Blackwell Publishing Asia, 40, (12) 895 - 898, 1996 |
| Improved method for preparation of samples for the polymerase chain reaction for detection of Coxiella burnetii in milk using immunomagnetic separation, Yasukazu Muramatsu; Minoru Maruyama; Tsuyoshi Yanase; Hiroshi Ueno; Chiharu Morita, Veterinary Microbiology, Elsevier B.V., 51, (1-2) 179 - 185, 1996 |
| The prevalence of Listeria monocytogenes in the environment of dairy farms, Hiroshi Ueno; Keiichi Yokota; Takanori Arai; Yasukazu Muramatsu; Hiroyuki Taniyama; Takashi Iida; Chiharu Morita, Microbiology and Immunology, Blackwell Publishing Asia, 40, (2) 121 - 124, 1996 |
| Seroepidemiological survey of wild rats for Seoul virus in Indonesia, Ima-Nurisa Ibrahim; Mohammad Sudomo; Chiharu Morita; Sachiko Uemura; Yasukazu Muramatsu; Hiroshi Ueno; Takashi Kitamura, Japanese Journal of Medical Science and Biology, National Institute of Health, 49, (2) 69 - 74, 1996 |
| Epidemiological survey of small rodents for spotted fever rickettsial antibody in Hokkaido, Japan, Tamaki Okabayashi; Nozomi Miura; Singo Miyazaki; Tomomi Nigo; Yasukazu Muramatsu; Hiroshi Ueno; Chiharu Morita, Japanese Journal of Medical Science and Biology, National Institute of Health, 49, (2) 63 - 68, 1996 |
| Does coinfection of Bartonella henselae and FIV induce clinical disorders in cats?, Hiroshi Ueno; Tsutomu Hohdatsu; Yasukazu Muramatsu; Hiroyuki Koyama; Chiharu Morita, Microbiology and Immunology, Blackwell Publishing Asia, 40, (9) 617 - 620, 1996 |
| Seroepidemiological Survey of Bartonella (Rochalimaea) henselae in Domestic Cats in Japan, Hiroshi Ueno; Yasukazu Muramatsu; Chiharu Morita; Bruno B. Chomel; Tsutomu Hohdatsu; Hiroyuki Koyama, Microbiology and Immunology, 39, (5) 339 - 341, 1995 |
| Amino acid polymorphisms of PrP with reference to onset of scrapie in Suffolk and Corriedale sheep in Japan, T. Ikeda; M. Horiuchi; N. Ishiguro; Y. Muramatsu; G. D. Kai-Uwe; M. Shinagawa, Journal of General Virology, Microbiology Society, 76, (10) 2577 - 2581, 1995 |
| Detection of PrPSc in sheep at the preclinical stage of scrapie and its significance for diagnosis of insidious infection, Y. Muramatsu; A. Onodera; M. Horiuchi; N. Ishiguro; M. Shinagawa, Archives of Virology, Springer-Verlag, 134, (3-4) 427 - 432, 1994 09 |
| Seroepidemiological Survey of Coxiella burnetii in Domestic Cats in Japan, Chiharu Morita; Tsutomu Hohdatsu; Hiroyuki Koyama; Jun Katsuyama; Tsuyoshi Yanase; Hiroshi Ueno; Yasukazu Muramatsu, Microbiology and Immunology, 38, (12) 1001 - 1003, 1994 |
| The Significance of PrPSc Detection for the Diagnosis of Insidious Scrapie, Y. MURAMATSU; A. ONODERA; M. HORIUCHI; N. ISHIGURO; M. SHINAGAWA, Annals of the New York Academy of Sciences, 724, (1) 347 - 349, 1994 |
| Isolation of Scrapie Agent from the Placenta of Sheep with Natural Scrapie in Japan, Onodera Takashi; Ikeda Toshio; Muramatsu Yasukazu; Shinagawa Morikazu, Japanese Journal of Microbiology, Center For Academic Publications Japan, 37, (4) 311 - 316, 1993 |
| A specific RFLP type associated with the occurrence of sheep scrapie in Japan, Y. Muramatsu; K. Tanaka; M. Horiuchi; N. Ishiguro; M. Shinagawa; T. Matsui; T. Onodera, Archives of Virology, Springer-Verlag, 127, (1-4) 1 - 9, 1992 03 |
Books etc
| 公衆衛生学 ; 動物看護関連法規 ; 動物愛護・適正飼養関連法規, 日本動物保健看護系大学協会カリキュラム委員会, 第3章第1節、第4節, EDUWARD Press, 2022 02 , 9784866711591 |
| 公衆衛生学 : 動物医療関連法規, 日本動物保健看護系大学協会カリキュラム委員会, 第3章第1節、第4節, インターズー, 2019 12 , 9784866710891 |
| 動物公衆衛生学 改訂版, 村松 康和, インターズー, 2019 12 |
| Q fever, Yasukazu MURAMATSU, 日本小児医事出版社, 2017 12 |
| 獣医公衆衛生研究, 村松 康和, 獣医大学からのお便り③, 全国公衆衛生獣医師協議会, 2017 03 |
| 化学療法の領域, 村松 康和, Q熱, 医薬ジャーナル社, 2017 02 |
| 動物公衆衛生学, 村松 康和, pp. 45-48, 89-98, 109-113., インターズー, 2015 12 |
| 健康教室, 村松 康和, ペット由来の感染症 pp.25-30., 東山書房, 2015 11 |
| 獣医公衆衛生学Ⅱ, 村松 康和, 紅斑熱群 pp59-60. Q熱 pp.86-88., 文永堂出版, 2014 05 |
Conference Activities & Talks
| Whole-genome analysis of two novel Coxiella burnetii strains isolated from different bovine breeds., 成田駿; 小野寺杏佳; 内田玲麻; 村松康和, 第72回日本ウイルス学会学術集会 |
| 乳牛および肉牛由来Coxiella burnetii株の分離, 小野寺杏佳; 成田駿; 内田玲麻; 村松康和, 第168回日本獣医学会学術集会 |
| Characterization and serological test of Aedes galloisi flavivirus (AGFV) and AGFV-like virus isolated from mosquitoes in Hokkaido, ○富久藍; 蒔田凪; Vincen; Ross Vicencio; 村松康和; 内田玲麻, The 71st Annual Meeting of the Japanese Society for Virology |
| The effects of the amino acid polymorphisms surrounding the N-linked glycosylation site of ZIKA E protein on the adaptation to mosquitoes, ○島崎晴輝; 下岡誠 Vincen; Ross Vicencio; 村松康和; 内田玲麻, The 71st Annual Meeting of the Japanese Society for Virology |
| セラピー犬の口腔内から分離されたBergeyella zoohelcumの薬剤感受性調査, 野口桃子; 平澤朋卯子; 内田玲麻; 村松康和, 第167回 日本獣医学会学術集会 |
| セラピー犬由来Bergeyella zoohelcum分離株の薬剤感受性に関する疫学調査, 野口桃子; 平澤朋卯子; 内田玲麻; 村松康和, 令和6年度日本獣医公衆衛生学会 北海道地区学会 |
| 黒毛和牛からのCoxiella burnetiiの検出とその遺伝子型, 谷田隆太郎; 西寒水将; 片岡妃奈; 内田玲麻; 村松康和, 令和5年度日本獣医公衆衛生学会 北海道地区学会 |
| 牛飼育環境からのCoxiella burnetii の検出と遺伝子型別, 星山弘行; 内田玲麻; 村松康和, 第164回日本獣医学会学術集会案内 |
| 北海道産ヤマトヤブカにおけるジカウイルス感受性の評価, 川江 詩乃; 岩本 夏奈; 江下 優樹; 大場 靖子; 澤 洋文; 村松 康和; 内田 玲麻, 第164回日本獣医学会学術集会 |
| 北海道のヤブカにおけるアルボウイルスの検索, 内田 玲麻; 櫻井 愛美; 村松 康和, 第164回日本獣医学会学術集会 |
| ヤマトヤブカ体内におけるジカウイルスのQuasispecies評価, 三木 要; 岩本 夏奈; 江下 優樹; 大場 靖子; 澤 洋文; 村松 康和; 内田 玲麻, 第164回日本獣医学会学術集会 |
| 飼い主と飼育犬における口腔内細菌の検出頻度と細菌叢の比較, ○村松 康和、井谷 寛、野村 美月、内田 玲麻、郡山 尚紀, 第164回日本獣医学会学術集会 |
| 「人の生活に身近な野生動物、伴侶動物と共生する微生物」, 村松康和, （一社）札幌市小動物獣医師会 野生動物委員会主催セミナー |
| セラピードッグとその飼い主における口腔内細菌叢の比較, 西川泰代; 郡山尚紀; 堀江一樹; 内田玲麻; 村松康和, 第162回日本獣医学会学術集会 |
| 飼育犬とその飼い主の口腔内細菌叢比較調査におけるMALDI-TOF MSシステムによる菌種同定法の有用性, 堀江一樹; 郡山尚紀; 西川泰代; 内田玲麻; 村松康和, 第162回日本獣医学会学術集会 |
| 北海道のマダニにおける吸血源とマダニ媒介性ウイルスの調査, 小柳優奈; 内田玲麻; 村松康和, 第162回日本獣医学会学術集会 |
| 飼い主とその飼育犬に関する口腔内細菌叢の相互関連性調査, 西川泰代; 郡山尚紀; 堀江一樹; 内田玲麻; 村松康和, 令和元年度北海道地区学会 |
| ヒト由来口腔内細菌叢との比較に基づく犬由来口腔内細菌叢調査におけるMALDI-TOF MSシステムを用いた菌種同定法の意義, 堀江一樹; 郡山尚紀; 西川泰代; 内田玲麻; 村松康和, 令和元年度北海道地区学会 |
| MALDI Biotyperを用いた健康牛の乳汁中における細菌の解析, 惠之巍; 村田亮; 大塚浩通; 内田玲麻; 村松康和, 第8回家畜感染症学会 |
| 豚由来Pasteurella multocida分離菌株における分子疫学調査, 村松康和; Tran Thi Nhat; 佐藤百合菜; 惠之巍; 内田玲麻, 平成30年度北海道地区学会 |
| オホーツク管内で捕獲されたエゾシカ口腔内からの網羅的な細菌検出, 内海 博貴; 折原 詳; 内田 玲麻; 黒澤 拓也; 横山 敦志; 村松 康和, 第160回 日本獣医学会学術集会 |
| と畜場における豚の肺膿瘍に関する分子疫学調査, 中山 大輔; 内田 玲麻; 黒澤 拓也; 井上 陽介; 山 奈津子; Thi Nhat Tran; 村松 康和 |
| Single nucleotide polymorphisms解析に基づくウシ胎盤由来Coxiella burnetii DNAの遺伝子型別, 村松 康和, 第159回日本獣医学会学術集会 |
| Multiple-locus variable-number tandem repeat analysisに基づくウシ胎盤から検出したCoxiella burnetii DNAの遺伝子型別, 村松 康和, 日本獣医師会北海道地区学会 |
Misc
| Comparing Educational Effects for Veterinary Students Between Virtual Reality-Based and On-Site Based Practices on Learning of Meat Inspection for Cattle, 松山亮太; 内田玲麻; 千里今日子; 浮田真琴; 福田昭; 権平智; 福森理加; 臼井優; 樋口豪紀; 及川伸; 村松康和; 蒔田浩平, 獣医疫学雑誌, 28, 2024 |
| 北海道産ヤマトヤブカにおけるジカウイルス感受性の評価, 川江詩乃; 岩本夏奈; 江下優樹; 大場靖子; 澤洋文; 村松康和; 内田玲麻, 日本獣医学会学術集会講演要旨集, 164th (CD-ROM), 2021 |
| ヤマトヤブカ体内におけるジカウイルスのQuasispecies評価, 三木要; 岩本夏奈; 江下優樹; 大場靖子; 澤洋文; 村松康和; 内田玲麻, 日本獣医学会学術集会講演要旨集, 164th (CD-ROM), 2021 |
| 飼い主とその飼育犬に関する口腔内細菌叢の相互関連性調査, 西川泰代; 郡山尚紀; 堀江一樹; 内田玲麻; 村松康和, 北海道獣医師会雑誌, 63, 2019 |
| ヒト由来口腔内細菌叢との比較に基づく犬由来口腔内細菌叢調査におけるMALDI-TOF MSシステムを用いた菌種同定法の意義, 堀江一樹; 郡山尚紀; 西川泰代; 内田玲麻; 村松康和, 北海道獣医師会雑誌, 63, 2019 |
| 飼育犬とその飼い主の口腔内細菌叢比較調査におけるMALDI-TOF MSシステムによる菌種同定法の有用性, 堀江一樹; 郡山尚紀; 西川泰代; 内田玲麻; 村松康和, 日本獣医学会学術集会講演要旨集, 162nd, 2019 |
| セラピードッグとその飼い主における口腔内細菌叢の比較, 西川泰代; 郡山尚紀; 堀江一樹; 内田玲麻; 村松康和, 日本獣医学会学術集会講演要旨集, 162nd, 2019 |
| Q & A 公衆衛生編, Yasukazu MURAMATSU, Journal of Japan Veterinary Medical association, 71, (3) 129 - 131, 2018 03 |
| MALDI-TOF質量分析システムによる犬口腔内からのBergeyella zoohelcumの検出, 原谷那美; 内田玲麻; 郡山尚紀; 鈴木章夫; 堀内基広; 村松康和, 北海道獣医師会雑誌, 59, 2015 |
| イヌ口腔内からのBergeyella zoohelcumの分離, 村松康和; 原谷那美; 内田玲麻; 郡山尚紀; 鈴木章夫; 堀内基広, 日本獣医学会学術集会講演要旨集, 158th, 2015 |
| C-3 下北半島に生息するニホンザル（Macaca fuscata）の寄生虫症および感染症に関する疫学調査, 浅川 満彦; 萩原 克郎; 村松 康和; 岡本 実; 渡辺 洋子; 三觜 慶, 霊長類研究所年報, 44, (44) 102 - 103, 2014 12 04 |
| C-4 下北半島に生息するニホンザル (Macaca fuscata) の寄生虫症および感染症に関する疫学調査, 浅川 満彦; 渡辺 洋子; 萩原 克郎; 村松 康和; 岡本 実; 後藤 俊二, 霊長類研究所年報, (43) 112 - 112, 2013 11 |
| 北海道の飼育犬におけるCapnocytophaga属菌の侵淫調査, HATANAKA YUI; TAKIGUCHI MITSUYOSHI; GOTO MASAMITSU; MURAMATSU YASUKAZU, 日本獣医学会学術集会講演要旨集, 154th, 2012 08 31 |
| 高速相同性検索ソフトUSEARCHを用いた乳牛糞便DNA-Illumina分析データのメタゲノム解析, 遠藤大二; 北市みのり; 村松康和; 杉村逸郎; 林正信, 日本獣医学会学術集会講演要旨集, 153rd, 2012 |
| 鳥類の感染・寄生虫症モニタリング調査に基盤を置く保全医学研究 : 私立大学戦略的研究基盤形成支援事業2008年度～2010年度の概要, 浅川 満彦; 遠藤 大二; 萩原 克郎; 村松 康和; 寺岡 宏樹; 松田 一哉; 岡本 実; 谷山 弘行; 田村 豊; 横田 博, 鳥類臨床研究会報, (14) 25 - 28, 2011 12 |
| Antimicrobial Susceptibility of Escherichia coli and Klebsiella spp. Isolated from Clinical Bovine Mastitis in the Ishikari Area of Hokkaido, SAKEMI Yoko; OKAKOI Masaaki; SHINODA Kojiro; MURAMATSU Yasukazu; UENO Hiroshi; TAMURA Yutaka, Journal of the Japan Veterinary Medical Association, 63, (3) 215 - 218, 2010 03 20 , 乳房炎由来細菌の薬剤感受性状況と、薬剤耐性化による公衆衛生上のリスクを評価する目的で、平成18年6月〜9月に北海道石狩地域で発症した牛乳房炎由来Escherichia coliおよび、Klebsiella属菌について薬剤感受性試験を行った。抗菌剤は、動物用および医療用の18種類を供試した。供試した106株のE. coli中29.2％、34株のKlebsiella属菌中91.2％に耐性株を認めたが、E. coliは家畜由来指標細菌の耐性率より低く、E. coli、Klebsiella属菌ともに二次選択薬である動物用抗菌剤に対してすべて感受性であった。したがって、該当地域における牛乳房炎由来E. coliおよびKlebsiella 属菌の人の健康への直接的な影響は少ないと考えられた。 |
| 動物病院におけるメチシリン耐性黄色ブドウ球菌の伝播, 田村豊; 村松康和; 中島千絵; 柳沢千恵; 鈴木定彦; 花木秀明, 感染症学雑誌, 82, 2008 |
| 日本の種雄牛におけるBSE感受性に関するプリオン遺伝子多型の出現頻度, 村松康和; 高橋健一; 早川宏之; 堀内基広; 上野弘志; 田村豊, 日本獣医学会学術集会講演要旨集, 144th, 2007 |
| 北海道のウシにおけるプリオン遺伝子の多形性, 村松康和; 堀内基広; 田村豊, 日本獣医学会学術集会講演要旨集, 141st, 2006 |
| ベトナムの酪農牛におけるプリオン遺伝子の多形性, 村松康和; 要田正治; 小河孝; 堀内基広; 田村豊, 日本獣医学会学術集会講演要旨集, 141st, 2006 |
| モンゴル在来ヒツジからのピロプラズマ目原虫DNAの検出, 村松康和; 八幡陽介; 堀内基広; 石黒直隆; 品川森一; 森田千春; 田村豊, 日本獣医学会学術集会講演要旨集, 140th, 2005 |
| Ehrlichia ruminantium, Sudan [3], Yasukazu Muramatsu; Shin-Ya Ukegawa; Abdel Rahim Mohamed El Hussein; Magdi Badawi Abdel Rahman; Khalil Mohamed Ali Abdel Gabbar; Agnes Mumbi Chitambo; Tomoyoshi Komiya; Enala Tembo Mwase; Chiharu Morita; Yutaka Tamura, Emerging Infectious Diseases, 11, (11) 1792 - 1793, 2005 , 10.3201/eid1111.050744 |
| A Preliminary Report of Parasitological and Microbiological survey of Freeranging Japanese Macaques (Macaca fuscata (Blyth)) in Boso Peninsula, Japan, SATOYOSHI Ayako; KABAYA Hajime; HAGIWARA Ko; TANIYAMA Hiroyuki; YOSHIZAWA Kazunori; TUJI Masayoshi; HAGIWARA Kasturo; MURAMATSU Yasukazu; ASAKAWA Mitsuhiko, Japanese Journal of Zoo and Wildlife Medicine, 9, (2) 79 - 83, 2004 09 01 , 10.5686/jjzwm.9.79, Because alien macaques (probably, Macaca mulatta) occur in the southern part of the Boso Peninsula, Japan, there is a possibility that their parasites and/or other pathogens may infect Japanese macaques (M.fuscata). To verify the possibility, this study was conducted mainly on parasites that included protozoan, helminth and arthropod, and other pathogens including Rickettsia and the Borna virus of 50 free ranging Japanese macaques in the peninsula. Consequently, 5 arthropod and nematode parasite species common to the Japanese macaque, namely Pedicinus obtusus, P.eurygaster, Streptopharagus pigmentatus, Strongyloides fulleborni, and Trichuris trichiura, were obtained in this survey. It is the first record of sites where Pedicinus spp. are seen in a host species.Coccidia and Babesia sp. were not obtained. Coxciella and the Borna virus from the same host samples will be published elsewhere. |
| Detection of antibodies against spotted fever group Rickettsia (SFGR), typhus group Rickettsia (TGR), and Coxiella burnetii in human febrile patients in the Philippines, Gerry Amor Camer; Marissa Alejandria; Miguel Amor; Hiroshi Satoh; Yasukazu Muramatsu; Hiroshi Ueno; Chiharu Morita, Japanese Journal of Infectious Diseases, 56, (1) 26 - 28, 2003 02 |
| 新生子マウスの腸粘膜上皮細胞へのプリオン伝達の試み, 岡本実; 古岡秀文; 堀内基弘; 野口敬紀; 萩原克郎; 村松康和; 辻正義; 朝長啓造; 生田和良, 日本獣医学会学術集会講演要旨集, 136th, 2003 |
| 大阪における野生シカの紅斑熱群リケッチアに対する血清抗体調査及び遺伝子的検出, 岡林 環樹; 石塚 譲; 大谷 新太郎; 川井 裕史; 山田 倫章; 佐藤 弘; 村松 康和; 上野 弘志; 森田 千春, 日本獣医学会学術集会講演要旨集, 127回, 1999 03 |
| An example of Ehrlichia disease in dog., 津山慎吾; 山内秀夫; 石坂智路; 久末正晴; 鄭光一; 村松康和; 森田千春; 長谷川篤彦; 辻本元, 日本獣医学会学術集会講演要旨集, 126th, 1998 08 |
| Seroepidemiological Study of Coxiella burnetii in Cervus nippon in Northern Japan, YASUMOTO Morihiro; YANASE Tsuyoshi; MURAMATSU Yasukazu; UENO Hiroshi; MORITA Chiharu, Japanese Journal of Zoo and Wildlife Medicine, 2, (2) 101 - 106, 1997 09 01 , Q-fever, caused by Coxiella(C) burnetii, is one of the most important zoonoses. In the present study, the antibody against C.burnetii was analyzed by indirect immunofluorescence antibody(IFA) test in 168 individuals of Japanese deer (Cervus nippon) to determine the prevalence of Q-fever in wild deer of northern Japan. Specific antibody against C.burnetii was found in 72(42.9%) deer, including 24 of 46(52.2%) in Hokkaido and 48 of 122(39.3%) in Tohoku. In Toya and Shari, the positive rate between adult(66.7%) and immature animals(17.6%) was significant (p<0.01). In adult deer, a significant difference was also noted between Hokkaido and Tohoku(p<0.01). |
| SURVEY OF NATURAL SCRAPIE IN JAPAN - ANALYSIS OF RFLP TYPES OF THE PRP GENE AND DETECTION OF PRPSC MAINLY IN SUFFOLK SHEEP, A ONODERA; T IKEDA; M HORIUCHI; N ISHIGURO; M ONUMA; N HIRANO; T MIKAMI; E HONDA; K HIRAI; K KAI; H YUGI; Y MURAMATSU; M SHINAGAWA, JOURNAL OF VETERINARY MEDICAL SCIENCE, 56, (4) 627 - 632, 1994 08 , 10.1292/jvms.56.627 |
| FAMILIAL SCRAPIE CASES WITH SHORTENED INCUBATION PERIODS, M SHINAGAWA; Y MURAMATSU; A ONODERA; T MATSUI; M HORIUCHI; N ISHIGURO; M NAKAGAWA, JOURNAL OF VETERINARY MEDICAL SCIENCE, 55, (4) 665 - 667, 1993 08 , 10.1292/jvms.55.665 |
Awards & Honors
| 2019 09 , 北海道獣医師会, 日本獣医公衆衛生学会（北海道）奨励賞, 飼い主とその飼育犬に関する口腔内細菌叢の相互関連性調査 |
| 2008 09 , 北海道獣医師会, 平成19年度 北海道獣医師会 会長賞 |
Research Grants & Projects
| Ministry of Health, Labour and Welfare, 厚生労働科学研究費補助金（食品の安全確保推進研究事業）, Pilot study conducible to build a support model for meat inspection by the diagnostic imaging based on the artificial intelligence technology, 202504, 202803 |
| MEXT, Japan, Grant-in-Aid for Scientific Research, アニマルセラピーにおける動物由来感染症起因細菌の網羅的検索, 201704, 202003, Yasukazu MURAMATSU |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, The research for ansector of methicillin-resistant Staphylococcus pseudintermedius among dogs in Japan, 20140401, 20180331, Ishihara Kanako; TAMURA Yutaka; MURAMATSU Yasukazu, The recent appearance of methicillin-resistant Staphylococcus pseudintermedius (MRSP) is a concern for both veterinary and human healthcare. The current study compared the molecular characteristics of 43 MRSP isolates from dogs in Japan with those of MRSP from previous reports using multilocus sequence typing, SCCmec typing, and detection of antimicrobial resistance genes. Three related clonal lineages, ST71, ST169, and the newly registered ST354, were observed amongst SCCmec II-III isolates from Japan, despite MRSP SCCmec II-III isolates being thought to belong to a single clonal lineage. The majority of SCCmec II-III isolates belonging to ST169 (9/11) and ST354 (3/3), but not ST71 (0/11), harboured tetM. Since the allele sequences of two loci (ack and sar) differ between ST71 and ST169 isolates, ST71 and ST169 clones have been derived from a common ancestral clone. ST354 is likely a variant clone of ST169. |
| 科学技術振興機構, 「食の安全」高度化を可能にする細菌叢プロファイリング法の開発と応用, 2008, 2008, 村松 康和, 主な食品媒介性病原細菌群の簡易、高感度かつ高特異的な検査系を確立し、「食の安全」高度化に貢献する。特許技術に基づ食品媒介性病原細菌群DNA検出プライマー最適化を行い、高感度・高特異的な細菌叢DNAプロファイリングにより、集約化・大規模化が急速に進む北海道酪農における、信頼度の高い「食の安全」の為の牛群管理高度化を目指す。 |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Epidemiological survey on the high-risk zoonosis in the southeast asian countries, 2005, 2007, TAMURA Yutaka; MURAMATSU Yasukazu, This study investigates to clarify the present situation of high-risk zoonosis to invade our country through an animal and food in the southeast asian countries. 1. Rabies virus (1) Indonesia : The N gene was detected from a dog brain that suffered rabies, and resembled that of isolates in China and Philippines. (2) Vietnam : The N gene was not detected from bat brain, but neutralizing antibody for rabies virus was found in dog serum. 2. Q fever rickettsia (1) Thailand : An antibody for Q fever was detected from chicken and cattle serum, and detected Coxiella DNA from ticks attached to cattle. (2) Indonesia : An antibody for Q fever was detected from chicken and cattle serum. (3) Vietnam : An antibody for Q fever was detected from chicken and cattle serum, and Coxiella DNA was detected from chicken and cattle. 3. Drug resistant bacteria (1) Thailand : A lot of multiple drug-resistant Enterococci were isolated from chicken feces. (2) Indonesia: Multiple drug-resistant Enterococci was isolated from chicken feces, and a lot of vancomycin resistant Enterococci (VRE) were isolated. (3) Vietnam : A lot of multiple drug-resistant Enterococci were isolated from chicken and cattle feces. From these results, rabies virus, Coxiella burnetti and drug resistant bacteria including VRE spread in the southeast asian countries which surrounded our country, and the possibility exists to invade our country through an animal and food. |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Experimental transmission of abnormal prion protein (PrPsc) in the small intestinal epithelial cells of neonatal mice, 2003, 2004, TANIYAMA Hiroyuki; HAGIWARA Katsurou; MURAMATSU Yasukazu, Using an immunohistochemical method, we attempted to detect the transmission of abnormal prion protein (PrPsc) to the enterocytes of the small intestine of neonatal mice by oral exposure with sheep brain affected by scrapie. Five 1-day-old neonatal mice were exposed by oral inoculation to the homogenized brain of a scrapie-affected sheep. In the small intestine of all mice 1 hour after inoculation, immunoreactivity with antinormal prion protein (PrPc) antibody was seen in the cytoplasm of viilus enterocytes. This finding suggests transmission of abnormal PrPsc into the cytoplasm of enterocytes. In control mice treated with normal sheep brain, no PrPc signal was seen in enterocytes of the small intestine. Immunopositivity for neurofilament protein and glial fibrillary acidic protein was seen in the cytoplasm of enterocytes of mice inoculated with scrapie and normal sheep brain. This suggests that the enterocytes of neonatal mice can absorb PrPsc and other macromolecular proteins of the sheep brain affected by scrapie and may be more important than previously thought as a pathway for PrPsc transmission in neonatal animals. After 7 days to 3months of oral inoculation to the homogenized brain of a scrapie-affected sheep, immunoreactivity with antinormal prion protein (PrPc) antibody was not seen in the cytoplasm of villus enterocytes and other tissues in the mucosa. The results suggest that the term of the transmission of abnormal priors protein (PrPsc) ineeds over 3 months and another system of the transmission is present in the mucosa of mouse. Lam inine reseptor (LR) and normal prion were more frequently present in the cytoplasm of enterocytes of suckling mice and young cattle than adult mice and cattle, thus young animals have the higher risk of transmission of abnormal prion than adult animals, via the gastrointestinal menbrane. |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Molecular phylogenic study of Ehrlichia spp. microorganisms as the etiological agents of emerging diseases in Japan, 2002, 2004, MURAMATSU Yasukazu, 1)Ehrlichial gene was detected from small rodents trapped in a Lyme disease-endemic area in Hokkaido, the northernmost island of Japan. Primer pairs of 168 rDNA targeting the genus of Ehrlichia and other region of the 16S rDNA specific for E. chaffeensis and E. muris were used for identification. The DNA fragment specific for 16S rDNA of E. spp. was detected from 4 of 94 Apodemus. speciosus mice (positive rate 4.3%) and 5 of 73 Clethrionomys rufocanus bedfordiae mice (positive rate 6.8%). The nucleotide sequence of the amplified 168 rDNA fragment was most similar to those of E muris-like ehrlichia, E spp HF565 and Shizuoka-36 originating in the northeastern part of Japan. In phylogenic analysis based on 16S rDNA sequences, these northeastern and southwestern groups of E. murrslike ehrlichia made a cluster with E. muris group microorganisms. These results suggest that there are two groups of E. murislike microorganisms in Japan. 2)A novel genotype of Ehrlichia rummantium, the causative agent of heartwater, was genetically detected in the Sudan. The new genotype was provisionally named as "Gedaref". The results of phylogenic analyses on the regions of pCS20 and major antigen protein I (map)) genes revealed that the ehrlichia) strain belongs to E. ruminantium-group, but does not correspond with any known strains of E. ruminantium. Furthermore, as the results of phylogenic analyses on map), "Gedaref" made clusters with several strains of E. ruminantium other than Um Banein, which had been known as the sole strain of E. ruminantium in the Sudan. This work provided the evidence that not only Um Banein strain but also the other strain exist in the Sudan. |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Etiological and molecular genetic surveillance of scrapie in Mongolia, 2000, 2002, HORIUCHI Motohiro; MURAMATSU Yasukazu; ISHIGURO Naotaka, Mongolia has a far long history for sheep-farming and is now farming over twelve million sheep. In the future, Mongolia has a potential for the major provider of livestock products among the world. Thus surveillance of prion diseases in animal in Mongolia will provide important information to evaluate the safety of livestock products in the country. During the research term, we visit to central, west, and east part of Mongolia, we did not get a positive indication on the existence of scrapie-suspected sheep in hearing. We also checked PrPSc in the medulla oblongata from sheep showed neurological symptoms, but all were negative for PrPSc. In order to investigate the diversity of PrP gene of sheep in Mongolia, we collected a total of 470 venous blood samples from 9 different breeds (Khalkh, Yeroo, Orkhon, Khangai, Sartuul, Govi-altai, Bayad, Darhad, and Symber-Karakul) and purified genomic DNA. PrP genotype (amino acid type) was determined by direct sequencing of PCR-amplified fragment corresponding to PrP open reading frame. With regard to PrP genotype at codon 171 determining susceptibility to scrapie, sheep carrying 171-Gln/Gln genotype that is associated with the susceptibility to scrape, varied from 58 to 97% among the breeds. Sheep carrying 171-Arg/Arg or 171-Arg/Gln genotype that confers resistance to scrapie infection were less abundant, especially only a small percentage of sheep possessed homozygous for 171-Arg alle. These results indicate sheep in Mongolia is composed rather of scrapie-susceptible population, so continuous surveillance as well as preventive measures against prion-infected animal are important to evaluate the risk of prion disease. These results are also useful for performing a selective breeding program that produce scrapie-resistant flock. |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Investigation of Listeria monocytogenes in organic farming environments based on detection of VBNC cells of the organism., 1998, 2000, UBNO Hiroshi, Listeria monocytogenes has a possibility to form VBNC (Viable But Non-Culturable) cells in natural environments, although knowledge of this phenomenon in the bacteria is very limitted. The author found that the suitable conditions for detecting VBNC cells of a test strain of L.monocytogenes were incubation at a concentration of 1/2 to 2/3 MIC in brain heart infusion for 4 to 8 hours after contact with levofloxacin. Because these conditions did not allow division of the test organism, but allowed formation of elongated VBNC cells, stained by Giemsa solution, in a liquid medium. About 10^7/ml of viable test organisms were added to sterilized soil solution, PBS and distilled water, and settled for 56 days at 0℃. During this period, plate counts and total bacterial counts were almost constant in sterilized soil solution, whereas VBNC cells were frequently (43% of numbers of total cells) found in 7 days after settlement. The similar results were obtained in PBS, although VBNC cells were seldom observed in distilled water. These results suggest that entry of L.monocytogenes organisms into VBNC state occurs at cold and starved conditions. After inoculating 6×10^7/ml of viable organisms in sterilized soil solution, the solution was concentrated by ten using PBS.Plate counts increased to 4.7×10^8/ml in the solution and almost same numbers (2.1×10^8/ml) of total bacterial counts were observed by an enzyme-linked immuno-sorbent assay (EIA) using peroxydase labeled L.monocytogenes antibodies. On the other hand, there was no stained organism in a sample taken from un-sterilized soil solution that yielded a plate count of 9.2×10^5/ml. These results suggest that L.monocytogenes in soil could be detected specifically by using the EIA. |
| 日本学術振興会, 科学研究費助成事業, リポ多糖解析に基づくcoxiella burnetiiの分子疫学的研究, 1997, 1998, 村松 康和, 北海道のウシの生乳から、マウスを用いて継代・分離した2株のCoxiella burnetii、Nine Mile II相菌(American Type Culture Collectionより購入;ATCC VR-616)、Califonia株(同;ATCC VR-614)、およびPricilla株(岐阜大学;平井克哉教授より分与)を発育鶏卵卵黄嚢内接種により大量培養した。次いでWilliams,J.C.et al.(1981)の方法に従いRenografinの密度勾配を用いた遠心分離によりC.burnetiiを精製した。 得られたコクシエラの精製菌を用いてSDS-PAGEを行った。次いで銀染色、およびWesternblot analysis(WBA)により、本研究による分離菌と、既存の各菌株との蛋白、および抗原性状に関する比較を行った。 銀染色の結果、本研究で得られた分離株には、比較に用いたNine Mile H相菌株、およびCalifornia株と異なる、株特異な蛋白画分は認められなかった。また、用いた全てのコクシエラで全蛋白の泳動像に差異は認められなかった。これらの結果は、国内外における以前の報告を裏付けるものである。 抗コクシエラ・マウス血清を用いたWBAの結果、分離2株中、1株において、他のコクシエラとは異なる抗原性状が認められた。現在、糖鎖の性状について検討中である。 血液吸着濾紙により採取した見かけ上健康なネコの乾燥濾紙血液を用いて、間接蛍光抗体法、およびPCRを行った。その結果、今回初めてPCRにより乾燥濾紙血液からのコクシエラ、およびネコひっかき病の主要な原因リケッチアであるBartonella henselaeに特異なDNAの検出に成功した。この事は乾燥濾紙血液サンプルが広範囲にわたるコクシエラの疫学調査に有用であることを示している(現在投稿準備中)。 |
| 日本学術振興会, 科学研究費助成事業, PCR-ELISAによるQ熱の診断に関する研究, 1995, 1995, 村松 康和, BGM細胞に0.001〜0.1%濃度の界面活性剤Triton X-100 (TX100)を加え、鏡検により細胞の破砕を観察した。Coxiella burnetii Nine Mile II相菌(ATCC VR-616)の培養上清(細胞外の菌体が主体)、及び感染BGM細胞(細胞内の菌体が主体)を別々に回収した。 培養上清について各々0.001〜0.01%濃度のTX100を加えた試料と無添加の試料を遠心後、PCRを行った結果、無添加の場合と検出感度に差は無かった。 感染細胞では0.01、0.02%のTX100を加え遠心した試料、免疫鉄ビーズで菌体を回収した(この方法についてはVeterinary microbiologyに掲載決定)。試料、及びTX100添加後、免疫鉄ビーズで菌体を回収した試料でPCRを行った。0.01%濃度のTX100を加えた試料では、TX100無添加で免疫鉄ビーズにより菌体回収した試料と比べPCRの結果、検出感度の上昇が見られた。また、0.01%TX100は免疫鉄ビーズと菌体の反応を阻害しないことが判った。牛乳の場合、0.01%TX100のみ添加では遠心後に大量の堅固なpelletが残り、PCRサンプル調整の妨げになると共に検出感度が低下した。牛乳からのPCR-ELISAサンプル調整では0.01%TX100添加+免疫鉄ビーズによる菌体回収で検出感度が良かった。 ウシ60頭の生乳から、この方法によりサンプル調整し、PCR-ELISAによりC. burnetii検出を行った。PCR-ELISAは先に提出した申請書に記載した方法に準じて行った。10頭が陽性で(陽性率16.7%)、うち5頭が強陽性であった。PCR-ELISAは高感度に、多数の試料の判定が出来る利点を持つ。(以上の結果について第121回日本獣医学会で発表予定。Applied and environmental microbiologyに投稿準備中。) |
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