School of Veterinary Medicine, Department of Veterinary Medicine
嶋田圭祐 シマダ ケイスケ
E-Mail：
Website：https://www.rakuno.ac.jp/archives/teacher/37054.htmlLast Update :2025/08/21Researcher Profile and Settings
Name
SHIMADA Keisuke
Affiliation (Master)
School of Veterinary Medicine, Department of Veterinary Medicine
Affiliation & Job
Rakuno Gakuen University School of Veterinary Medicine Department of Veterinary Medicine
Education
2012 04 - 2016 03 , Hokkaido University, Graduate School of Veterinary Medicine 2003 04 - 2009 03 , Hokkaido University, Faculty of Veterinary Medicine
Degree
Ph.D of Veterinary Medicine, Hokkaido University
Association Memberships
Japanese Association for Laboratory Animal Science (JALAS) Japan Society of Andrology Society for the Study of Reproduction (SSR) Japanese College of Laboratory Animal Medicine (JCLAM) Japanese Association for Laboratory Animal Medicine (JALAM) THE JAPANESE SOCIETY OF VETERINARY SCIENCE
Research Activities
Research Areas
Life sciences, Developmental biology Life sciences, Laboratory animal science
Research Interests
Biology of Reproduction, Laboratory Animal Science,
Published Papers
Compensation for X-linked Pdha1 silencing by Pdha2 is essential for meiotic double-strand break repair in spermatogenesis, Chen Pan; Keisuke Shimada; Hsin-Yi Chang; Haoting Wang; Masahito Ikawa, Development, 2025 08 01 Mouse genome engineering uncovers 18 genes dispensable for male reproduction, Hsin‐Yi Chang; Yonggang Lu; Kaito Yamamoto; Jiang Sun; Keisuke Shimada; Yuki Hiradate; Yoshitaka Fujihara; Masahito Ikawa, Andrology, 2025 06 26 The absence of both RIBC1 and RIBC2 induces decreased sperm motility and litter size in male mice, Kento Katsuma; Keisuke Shimada; Shingo Tonai; Daisuke Mashiko; Rie Iida‐Norita; Yuki Kaneda; Haruhiko Miyata; Masahito Ikawa, Andrology, 2025 04 23 TEX38 localizes ZDHHC19 to the plasma membrane and regulates sperm head morphogenesis in mice, Yuki Kaneda; Yonggang Lu; Jiang Sun; Keisuke Shimada; Chihiro Emori; Taichi Noda; Takayuki Koyano; Makoto Matsuyama; Haruhiko Miyata; Masahito Ikawa, Proceedings of the National Academy of Sciences, 2025 03 11 Loss of CCDC188 causes male infertility with defects in the sperm head-neck connection in mice., Yumiao Qiu; Keisuke Shimada; Kaito Yamamoto; Masahito Ikawa, Biology of reproduction, 2024 09 18 FBXO24 deletion causes abnormal accumulation of membraneless electron-dense granules in sperm flagella and male infertility., Yuki Kaneda; Haruhiko Miyata; Zoulan Xu; Keisuke Shimada; Maki Kamoshita; Tatsuya Nakagawa; Chihiro Emori; Masahito Ikawa, eLife, eLife Sciences Publications, Ltd, 13, 2024 08 20 MYCBPAP is a central apparatus protein required for centrosome-nuclear envelope docking and sperm tail biogenesis in mice., Haoting Wang; Hiroko Kobayashi; Keisuke Shimada; Seiya Oura; Yuki Oyama; Hiroaki Kitakaze; Taichi Noda; Norikazu Yabuta; Haruhiko Miyata; Masahito Ikawa, Journal of cell science, The Company of Biologists, 137, (16) , 2024 08 15 Development of functional spermatozoa in mammalian spermiogenesis., Haruhiko Miyata; Keisuke Shimada; Yuki Kaneda; Masahito Ikawa, Development (Cambridge, England), The Company of Biologists, 151, (14) , 2024 07 15 Individual disruption of 12 testis-enriched genes via the CRISPR/Cas9 system does not affect the fertility of male mice., Akira Suzuki; Norikazu Yabuta; Keisuke Shimada; Daisuke Mashiko; Keizo Tokuhiro; Yuki Oyama; Haruhiko Miyata; Thomas X Garcia; Martin M Matzuk; Masahito Ikawa, Journal of reproductive immunology, 163, 104252 - 104252, 2024 06 Gene-deficient mouse model established by CRISPR/Cas9 system reveals 15 reproductive organ-enriched genes dispensable for male fertility., Tuyen Thi Thanh Nguyen; Keizo Tokuhiro; Keisuke Shimada; Haoting Wang; Daisuke Mashiko; Shingo Tonai; Daiji Kiyozumi; Masahito Ikawa, Frontiers in cell and developmental biology, 12, 1411162 - 1411162, 2024 05 01 Affinity-tagged SMAD1 and SMAD5 mouse lines reveal transcriptional reprogramming mechanisms during early pregnancy., Zian Liao; Suni Tang; Kaori Nozawa; Keisuke Shimada; Masahito Ikawa; Diana Monsivais; Martin Matzuk, eLife, 12, 2024 03 27 Disruption of testis-enriched cytochrome c oxidase subunit COX6B2 but not COX8C leads to subfertility., Keisuke Shimada; Yonggang Lu; Masahito Ikawa, Experimental animals, Japanese Association for Laboratory Animal Science, 73, (1) 1 - 10, 2024 02 14 Multiple ageing effects on testicular/epididymal germ cells lead to decreased male fertility in mice., Tsutomu Endo; Kiyonori Kobayashi; Takafumi Matsumura; Chihiro Emori; Manabu Ozawa; Shimpei Kawamoto; Daisuke Okuzaki; Keisuke Shimada; Haruhiko Miyata; Kentaro Shimada; Mayo Kodani; Yu Ishikawa-Yamauchi; Daisuke Motooka; Eiji Hara; Masahito Ikawa, Communications biology, 7, (1) 16 - 16, 2024 01 04 CCDC183 is essential for cytoplasmic invagination around the flagellum during spermiogenesis and male fertility., Keisuke Shimada; Masahito Ikawa, Development (Cambridge, England), 150, (21) , 2023 11 01 Testis-specific proteins, TSNAXIP1 and 1700010I14RIK, are important for sperm motility and male fertility in mice., Yuki Kaneda; Haruhiko Miyata; Keisuke Shimada; Seiya Oura; Masahito Ikawa, Andrology, 11, (5) 799 - 807, 2023 07 TSKS localizes to nuage in spermatids and regulates cytoplasmic elimination during spermiation., Keisuke Shimada; Soojin Park; Seiya Oura; Taichi Noda; Akane Morohoshi; Martin M Matzuk; Masahito Ikawa, Proceedings of the National Academy of Sciences of the United States of America, 120, (11) , 2023 03 14 Evaluation of the efficacy of creatine chemical exchange saturation transfer imaging in assessing testicular maturity., Sohei Kuribayashi; Shinichiro Fukuhara; Go Tsujimura; Takahiro Imanaka; Koichi Okada; Norichika Ueda; Kentaro Takezawa; Hiroshi Kiuchi; Shigeyoshi Saito; Yusuke Takahashi; Hidetaka Kioka; Seiya Oura; Keisuke Shimada; Masahito Ikawa; Norio Nonomura, Reproductive medicine and biology, 22, (1) , 2023 02 IRGC1, a testis-enriched immunity related GTPase, is important for fibrous sheath integrity and sperm motility in mice., Yuki Kaneda; Haruhiko Miyata; Keisuke Shimada; Yuki Oyama; Rie Iida-Norita; Masahito Ikawa, Developmental biology, Elsevier BV, 488, 104 - 113, 2022 08 01 Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis., Shintaro Mise; Akinobu Matsumoto; Keisuke Shimada; Toshiaki Hosaka; Masatomo Takahashi; Kazuya Ichihara; Hideyuki Shimizu; Chisa Shiraishi; Daisuke Saito; Mikita Suyama; Tomoharu Yasuda; Toru Ide; Yoshihiro Izumi; Takeshi Bamba; Tomomi Kimura-Someya; Mikako Shirouzu; Haruhiko Miyata; Masahito Ikawa; Keiichi I Nakayama, Nature communications, 13, (1) 1071 - 1071, 2022 02 28 TULP2 deletion mice exhibit abnormal outer dense fiber structure and male infertility., Yuki Oyama; Haruhiko Miyata; Keisuke Shimada; Tamara Larasati; Yoshitaka Fujihara; Masahito Ikawa, Reproductive medicine and biology, Wiley, 21, (1) , 2022 05 01 LRRC23 is a conserved component of the radial spoke that is necessary for sperm motility and male fertility in mice, Xin Zhang; Jiang Sun; Yonggang Lu; Jintao Zhang; Keisuke Shimada; Taichi Noda; Shuqin Zhao; Takayuki Koyano; Makoto Matsuyama; Shushu Zhou; Jiayan Wu; Masahito Ikawa; Mingxi Liu, Journal of Cell Science, The Company of Biologists, 134, (20) , 2021 09 29 FAM209 associates with DPY19L2 and is required for sperm acrosome biogenesis and fertility in mice, Julio M. Castaneda; Keisuke Shimada; Yuhkoh Satouh; Zhifeng Yu; Masahito Ikawa; Martin M. Matzuk, Journal of Cell Science, The Company of Biologists, 134, (21) , 2021 09 02 SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice, Haruhiko Miyata; Seiya Oura; Akane Morohoshi; Keisuke Shimada; Daisuke Mashiko; Yuki Oyama; Yuki Kaneda; Takafumi Matsumura; Ferheen Abbasi; Masahito Ikawa, Proceedings of the National Academy of Sciences of the United States of America, 118, (35) , 2021 08 31 CRISPR/Cas9-mediated genome editing reveals 12 testis-enriched genes dispensable for male fertility in mice., Yuki Oyama; Haruhiko Miyata; Keisuke Shimada; Yoshitaka Fujihara; Keizo Tokuhiro; Thomas X Garcia; Martin M Matzuk; Masahito Ikawa, Asian journal of andrology, 24, (3) 266 - 272, 2021 07 16 Endometrial receptivity and implantation require uterine BMP signaling through an ACVR2A-SMAD1/SMAD5 axis, Diana Monsivais; Takashi Nagashima; Renata Prunskaite-Hyyryläinen; Kaori Nozawa; Keisuke Shimada; Suni Tang; Clark Hamor; Julio E. Agno; Fengju Chen; Ramya P. Masand; Steven L. Young; Chad J. Creighton; Francesco J. DeMayo; Masahito Ikawa; Se Jin Lee; Martin M. Matzuk, Nature Communications, 12, (1) , 2021 06 07 ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility, Keisuke Shimada; Soojin Park; Haruhiko Miyata; Zhifeng Yu; Akane Morohoshi; Seiya Oura; Martin M. Matzuk; Masahito Ikawa, Proceedings of the National Academy of Sciences of the United States of America, 118, (6) , 2021 02 09 Bi-allelic DNAH8 Variants Lead to Multiple Morphological Abnormalities of the Sperm Flagella and Primary Male Infertility, Chunyu Liu; Haruhiko Miyata; Yang Gao; Yanwei Sha; Shuyan Tang; Zoulan Xu; Marjorie Whitfield; Catherine Patrat; Huan Wu; Emmanuel Dulioust; Shixiong Tian; Keisuke Shimada; Jiangshan Cong; Taichi Noda; Hang Li; Akane Morohoshi; Caroline Cazin; Zine Eddine Kherraf; Christophe Arnoult; Li Jin; Xiaojin He; Pierre F. Ray; Yunxia Cao; Aminata Touré; Feng Zhang; Masahito Ikawa, American Journal of Human Genetics, 107, (2) 330 - 341, 2020 08 06 Tmprss12 is required for sperm motility and uterotubal junction migration in mice, Tamara Larasati; Taichi Noda; Yoshitaka Fujihara; Keisuke Shimada; Tomohiro Tobita; Zhifeng Yu; Martin M. Matzuk; Masahito Ikawa, Biology of Reproduction, 103, (2) 254 - 263, 2020 08 01 CRISPR/Cas9-mediated genome-edited mice reveal 10 testis-enriched genes are dispensable for male fecundity, Soojin Park; Keisuke Shimada; Yoshitaka Fujihara; Zoulan Xu; Kentaro Shimada; Tamara Larasati; Putri Pratiwi; Ryan M. Matzuk; Darius J. Devlin; Zhifeng Yu; Thomas X. Garcia; Martin M. Matzuk; Masahito Ikawa, Biology of Reproduction, 103, (2) 195 - 204, 2020 08 01 PHF7 Modulates BRDT Stability and Histone-to-Protamine Exchange during Spermiogenesis, Chang Rok Kim; Taichi Noda; Hyunkyung Kim; Gibeom Kim; Seongwan Park; Yongwoo Na; Seiya Oura; Keisuke Shimada; Injin Bang; Jun Yeong Ahn; Yong Ryoul Kim; Se Kyu Oh; Hee Jung Choi; Jong Seo Kim; Inkyung Jung; Ho Lee; Yuki Okada; Masahito Ikawa; Sung Hee Baek, Cell Reports, Elsevier {BV}, 32, (4) 107950 - 107950, 2020 07 28 Welfare Impact of Carbon Dioxide Euthanasia on Laboratory Mice and Rats: A Systematic Review, Patricia V. Turner; Debra L. Hickman; Judith van Luijk; Merel Ritskes-Hoitinga; Jan M. Sargeant; T. Miki Kurosawa; Takashi Agui; Vera Baumans; Woo Sung Choi; Yang Kyu Choi; Paul A. Flecknell; Byeong H. Lee; Pedro J. Otaegui; Kathleen R. Pritchett-Corning; Keisuke Shimada, Frontiers in Veterinary Science, 7, 2020 07 22 Testis-enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa, Haruhiko Miyata; Keisuke Shimada; Akane Morohoshi; Seiya Oura; Takafumi Matsumura; Zoulan Xu; Yuki Oyama; Masahito Ikawa, FASEB Journal, 34, (4) 5389 - 5400, 2020 04 Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice, Akane Morohoshi; Haruhiko Miyata; Keisuke Shimada; Kaori Nozawa; Takafumi Matsumura; Ryuji Yanase; Kogiku Shiba; Kazuo Inaba; Masahito Ikawa, PLoS Genetics, Public Library of Science ({PLoS}), 16, (1) , 2020 CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched genes dispensable for male fertility in mice, Yonggang Lu; Seiya Oura; Takafumi Matsumura; Asami Oji; Nobuyuki Sakurai; Yoshitaka Fujihara; Keisuke Shimada; Haruhiko Miyata; Tomohiro Tobita; Taichi Noda; Julio M. Castaneda; Daiji Kiyozumi; Qian Zhang; Tamara Larasati; Samantha A.M. Young; Mayo Kodani; Caitlin A. Huddleston; Matthew J. Robertson; Cristian Coarfa; Ayako Isotani; R. John Aitken; Masaru Okabe; Martin M. Matzuk; Thomas X. Garcia; Masahito Ikawa, Biology of Reproduction, Oxford University Press ({OUP}), 101, (2) 501 - 511, 2019 08 01 Glycerol kinase 2 is essential for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during mouse spermatogenesis, Keisuke Shimada; Hirotaka Kato; Haruhiko Miyata; Masahito Ikawa, Journal of Reproduction and Development, 65, (2) 155 - 162, 2019 Chimeric analysis with newly established EGFP/DsRed2-tagged ES cells identify HYDIN as essential for spermiogenesis in mice, Seiya Oura; Haruhiko Miyata; Taichi Noda; Keisuke Shimada; Takafumi Matsumura; Akane Morohoshi; Ayako Isotani; Masahito Ikawa, Experimental Animals, 68, (1) 25 - 34, 2019 RSPH6A is required for sperm flagellum formation and male fertility in mice, Ferheen Abbasi; Haruhiko Miyata; Keisuke Shimada; Akane Morohoshi; Kaori Nozawa; Takafumi Matsumura; Zoulan Xu; Putri Pratiwi; Masahito Ikawa, Journal of Cell Science, 131, (19) , 2018 10 01 Prolonged rest period enables the detection of micronucleated hepatocytes in the liver of young adult rats after a single dose of diethylnitrosamine or mitomycin C, Keisuke Shimada; Mika Yamamoto; Miyuki Takashima; Jiro Seki; Yoichi Miyamae; Akihiro Wakata, Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 791, 38 - 41, 2015 09 01 Repeated-dose liver micronucleus assay of mitomycin C in young adult rats, Keisuke Shimada; Mika Yamamoto; Miyuki Takashima; Yumi Wako; Kazufumi Kawasako; Yoshinobu Aoki; Jiro Seki; Yoichi Miyamae; Akihiro Wakata, Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 780-781, 85 - 89, 2015 03 01 Evaluation of the repeated-dose liver and gastrointestinal tract micronucleus assays with 22 chemicals using young adult rats: Summary of the collaborative study by the Collaborative Study Group for the Micronucleus Test (CSGMT)/The Japanese Environmental Mutagen Society (JEMS) - Mammalian Mutagenicity Study Group (MMS), Shuichi Hamada; Wakako Ohyama; Rie Takashima; Keisuke Shimada; Kazumi Matsumoto; Satoru Kawakami; Fuyumi Uno; Hajime Sui; Yasushi Shimada; Tadashi Imamura; Shoji Matsumura; Hisakazu Sanada; Kenji Inoue; Shigeharu Muto; Izumi Ogawa; Aya Hayashi; Tomomi Takayanagi; Yosuke Ogiwara; Akihisa Maeda; Emiko Okada; Yukari Terashima; Hironao Takasawa; Kazunori Narumi; Yumi Wako; Kazufumi Kawasako; Masaki Sano; Nobuyuki Ohashi; Takeshi Morita; Hajime Kojima; Masamitsu Honma; Makoto Hayashi, Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 780-781, 2 - 17, 2015 03 01
Conference Activities & Talks
TSKSは精子細胞内のnuageに局在し、細胞質内内容物の除去に関わることで流線型の精子形成を可能にする, 嶋田圭祐, 日本アンドロロジー学会 第44回学術大会 Molecular Mechanism Analysis Involved in Mitochondrial Sheath Formation, Keisuke Shimada, ASA/ICA 2025 Annual Meeting CCDC183 is Essential for Axonemal Compartmentalization during Spermiogenesis, Keisuke Shimada and Masahito Ikawa, SSR Annual meeting 2024 CCDC183は精子形成過程において軸糸微小管のコンパートメント化に必須である, 嶋田圭祐; 伊川正人, 日本アンドロロジー学会 第43回学術大会 CCDC183は精子形成過程における鞭毛周囲の細胞質陥入および軸糸微小管のコンパートメント化に必須である, 嶋田圭祐, 第71回日本実験動物学会総会 遺伝子改変マウスを用いた精子形成に関する分子メカニズム解析, 嶋田 圭祐, 実験動物札幌セミナー2023 Molecular mechanism analysis involved in mitochondrial sheath formation and spermiation processes using gene-manipulated mice, 嶋田 圭祐, IMP seminar at research Institute of Molecular Pathology TSKS-derived nuages regulate the transportation of cytoplasmic contents during spermiation, Shimada Keisuke; Park Soojin; Oura Seiya; Noda Taichi; Morohoshi Akane; Matzuk M. Martin; Ikawa Masahito, European testis workshop 2023 TSKSは精子細胞内の細胞質内内容物の輸送を制御することで流線型の精子形成を可能にする, 嶋田 圭祐; Park Soojin; 大浦聖矢; 野田大地; 諸星茜; Martin M. Matzuk; 伊川 正人, 第70回日本実験動物学会総会 ARMC12はTBC1D21と協働してミトコンドリア鞘形成を可能にする, 嶋田 圭祐; Park Soojin; 宮田治彦; Zhifeng Yu; 諸星茜; 大浦聖矢; Martin M. Matzuk; 伊川 正人, 第69回日本実験動物学会総会 ARMC12は精子形成期におけるミトコンドリア鞘形成に必須である, 嶋田圭祐, Soojin Park, 宮田治彦, Zhifeng Yu, 諸星茜, 大浦聖矢, Martin M. Matzuk, 伊川正人, 第163回日本獣医学会学術集会 遺伝子改変マウスを用いた精子鞭毛形成の解析, 諸星 茜、宮田 治彦、嶋田 圭祐、野澤 香織、松村 貴史、梁瀬 隆二、柴 小菊、稲葉 一男、伊川 正人, 新学術領域研究「全能性プログラム：デコーディングからデザインへ」若手勉強会2019 Molecular mechanism of mitochondrial sheath formation revealed through gene-edited mice, Keisuke Shimada, Haruhiko Miyata, Masahito Ikawa, 第14回研究所ネットワーク国際シンポジウム SMDR1は精子形成期においてミトコンドリア間を繋ぎ止めて精子中片部にミトコンドリア鞘を形成するのに必須のタンパク質である, 嶋田圭祐,宮田治彦,伊川正人, 第162回日本獣医学会学術集会 Glycerol kinase 2 is important for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath, Keisuke Shimada, Haruhiko Miyata, Masahito Ikawa, SSR 52nd Annual Meeting マウスRSPH6Aは精子鞭毛の形成と生殖能力に必須である, 宮田 治彦, Ferheen Abbasi, 嶋田 圭祐, 諸星 茜, 野澤 香織, 松村 貴史, Zoulan Xu, Putri Pratiwi, 伊川 正人, 日本アンドロロジー学会第38回学術大会 SMDR1は精子ミトコンドリアの鞭毛への巻き付きに必須である, 嶋田 圭祐, 宮田 治彦, 伊川 正人, 第33回日本生殖免疫学会 マウスDRC7は精子鞭毛形成および生殖能力に必須である, 諸星 茜、宮田 治彦、嶋田 圭祐、野澤 香織、松村 貴史、伊川 正人, 第41回日本分子生物学会年会 SMDR1は精子形成期における精子ミトコンドリアの鞭毛への巻き付きに関わることでミトコンドリア鞘の形成に必須となるタンパク質である, 嶋田 圭祐, 宮田 治彦, 伊川 正人, 第161回日本獣医学会学術集会 新規EGFP/DsRed2標識ES細胞を用いたゲノム編集とキメラマウスにおける表現型解析, 大浦 聖矢、宮田 治彦、野田 大地、嶋田 圭祐、松村 貴史、諸星 茜、磯谷 綾子、伊川 正人, 若手支援技術講習会2018 RSPH6A is essential for sperm flagellum formation and male fertility in mice, Haruhiko Miyata, Ferheen Abbasi, Keisuke Shimada, Akane Morohoshi, Kaori Nozawa, Takafumi Matsumura, Masahito Ikawa, 51st Annual Meeting of the Society for the Study of Reproduction マウスRSPH6Aは精子鞭毛の形成と生殖能力に必須である, 宮田治彦, Ferheen Abbasi, 嶋田圭祐, 松村貴史, 諸星茜, 伊川正人, 第65回日本実験動物学会総会 Smdr1 is required for sperm mitochondrial alignment during spermatogenesis, 嶋田圭祐,宮田治彦,伊川正人, The 1st International Mitochondria Meeting for Young Scientists Gk2 knockout mice show impaired sperm motility and male infertility, Keisuke Shimada, Hirotaka Kato, Haruhiko Miyata, Yuhkoh Satouh, Masahito Ikawa, 50th Annual Meeting of the Society for the Study of Reproduction Gk2 knockout mice show impaired sperm motility and male infertility, Keisuke Shimada, Hirotaka Kato, Haruhiko Miyata, Yuhkoh Satouh, Masahito Ikawa, Fertilization & Activation of Development (Gordon Research Conference) CRISPR/Cas9システムによるES細胞のゲノム編集とキメラマウス解析, 大字 亜沙美 ,野田 大地 ,嶋田 圭祐 ,伊川 正人, 平成28年度新学術領域研究「学術研究支援基盤形成【先端モデル動物支援プラットフォーム】」若手支援技術講習会
Awards & Honors
2025 03 , International Society of Andrology, ISA Young Andrologist Award 2024 06 , Japan society of androrology, 令和6年度 日本アンドロロジー学会学術奨励賞 2024 01 , 杉山記念財団, 第5回 SMF論文表彰制度 優秀賞 2022 05 , 公益社団法人 日本実験動物学会, 第69回日本実験動物学会総会 優秀発表賞 2019 01 , Research Institute for Microbial Diseases, Osaka University, Biken Best Academic Award
Research Grants & Projects
日本学術振興会, 科学研究費助成事業 基盤研究(C), 精子形成過程に出現する球状ミトコンドリアの形成メカニズムに迫る, 20230401, 20260331, 嶋田 圭祐 Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists, Analysis of mitochondrial sheath formation mechanism using KO mice, 202004, 202303, 嶋田 圭祐, 本研究ではSmdr3及びSmdr5遺伝子を欠損させたKOマウスの表現型を解析し，これら遺伝子がどのようにミトコンドリア鞘形成に関与しているのかを明らかにすることを目的としている。
SMDR3に対する抗体を作成し，免疫電子顕微鏡観察をしたところ，SMDR3は予想に反してミトコンドリアとは独立した2種類のnuageに局在していることが明らかになった。このnuageはpiRNAが局在するIntermitochondrial cementとは異なるnuageであり，これまでにその機能については全く解析されてこなかったものであった。本研究によりSMDR3に由来するnuageが精子ミトコンドリアの整列に関連することが明らかになった。SMDR3を培養細胞に過剰発現させると，SMDR3は単独で細胞質内にdropletを形成することから，SMDR3は細胞内に液-液相分離を引き起こすことで機能していることも明らかになった。
Smdr5 KOマウスは精子形成過程で精子ミトコンドリアの内部構造に異常を呈し，ミトコンドリア鞘形成がうまくいかないことで雄性不妊となる。今回Smdr5を生殖細胞のみで発現するトランスジェニック（Tg）マウスを作成し、Smdr5 KOマウスに導入すると、雄性不妊がレスキューできることが明らかになった。さらにユビキタスに発現するSmdr5のファミリー遺伝子についても、生殖細胞のみで発現するTgマウスを作成し、Smdr5 KOマウスに導入しても雄性不妊はレスキューできたため，SMDR5の機能はそのファミリータンパク質と同様の働きを有していることが考えられた。したがって，これら2つのタンパク質は発現場所のみ違いがあるものの，機能は同じタンパク質であることが示唆された。 Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Revealing the mechanism of mitochondrial localization in the sperm midpiece, 201704, 202003, Keisuke Shimada, We discovered that absence of Sperm mitochondria related gene 1(SMDR1) disrupts mitochondrial sheath formation, resulting in reduced sperm motility and male sterility. In vitro analysis demonstrates that SMDR1 induces mitochondrial clustering, revealing that SMDR1 functions as an adherence factor between mitochondria. In mouse testicular germ cells, SMDR1 localizes to the mitochondrial surface and directly interacts with mitochondrial outer membrane proteins VDAC2 and VDAC3 as well as SMDR2. These findings suggest that SMDR1 binds to VDAC proteins to tether adjacent mitochondria, and this tethering allows mitochondria to properly elongate and coil around the sperm flagellum. Thus, our studies demonstrate for the first time that SMDR1 regulates sperm mitochondrial dynamics, an essential function for mitochondrial sheath formation and male fertility. 公益財団法人千里ライフサイエンス振興財団, 岸本基金研究助成, 精子形成期後半に発生する 2 つの異なる nuage の機能と役割の解明 公益財団法人 武田科学振興財団, 医学系研究助成（基礎）, Elucidation of molecular mechanisms involved in sperm mitochondrial dynamics
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Last Update :2025/08/21
Researcher Profile and Settings
Name
| SHIMADA Keisuke |
Affiliation (Master)
| School of Veterinary Medicine, Department of Veterinary Medicine |
Affiliation & Job
| Rakuno Gakuen University |
| School of Veterinary Medicine Department of Veterinary Medicine |
Education
| 2012 04 - 2016 03 , Hokkaido University, Graduate School of Veterinary Medicine |
| 2003 04 - 2009 03 , Hokkaido University, Faculty of Veterinary Medicine |
Degree
| Ph.D of Veterinary Medicine, Hokkaido University |
Association Memberships
| Japanese Association for Laboratory Animal Science (JALAS) |
| Japan Society of Andrology |
| Society for the Study of Reproduction (SSR) |
| Japanese College of Laboratory Animal Medicine (JCLAM) |
| Japanese Association for Laboratory Animal Medicine (JALAM) |
| THE JAPANESE SOCIETY OF VETERINARY SCIENCE |
Research Activities
Research Areas
| Life sciences, Developmental biology |
| Life sciences, Laboratory animal science |
Research Interests
| Biology of Reproduction, |
| Laboratory Animal Science, |
Published Papers
| Compensation for X-linked Pdha1 silencing by Pdha2 is essential for meiotic double-strand break repair in spermatogenesis, Chen Pan; Keisuke Shimada; Hsin-Yi Chang; Haoting Wang; Masahito Ikawa, Development, 2025 08 01 |
| Mouse genome engineering uncovers 18 genes dispensable for male reproduction, Hsin‐Yi Chang; Yonggang Lu; Kaito Yamamoto; Jiang Sun; Keisuke Shimada; Yuki Hiradate; Yoshitaka Fujihara; Masahito Ikawa, Andrology, 2025 06 26 |
| The absence of both RIBC1 and RIBC2 induces decreased sperm motility and litter size in male mice, Kento Katsuma; Keisuke Shimada; Shingo Tonai; Daisuke Mashiko; Rie Iida‐Norita; Yuki Kaneda; Haruhiko Miyata; Masahito Ikawa, Andrology, 2025 04 23 |
| TEX38 localizes ZDHHC19 to the plasma membrane and regulates sperm head morphogenesis in mice, Yuki Kaneda; Yonggang Lu; Jiang Sun; Keisuke Shimada; Chihiro Emori; Taichi Noda; Takayuki Koyano; Makoto Matsuyama; Haruhiko Miyata; Masahito Ikawa, Proceedings of the National Academy of Sciences, 2025 03 11 |
| Loss of CCDC188 causes male infertility with defects in the sperm head-neck connection in mice., Yumiao Qiu; Keisuke Shimada; Kaito Yamamoto; Masahito Ikawa, Biology of reproduction, 2024 09 18 |
| FBXO24 deletion causes abnormal accumulation of membraneless electron-dense granules in sperm flagella and male infertility., Yuki Kaneda; Haruhiko Miyata; Zoulan Xu; Keisuke Shimada; Maki Kamoshita; Tatsuya Nakagawa; Chihiro Emori; Masahito Ikawa, eLife, eLife Sciences Publications, Ltd, 13, 2024 08 20 |
| MYCBPAP is a central apparatus protein required for centrosome-nuclear envelope docking and sperm tail biogenesis in mice., Haoting Wang; Hiroko Kobayashi; Keisuke Shimada; Seiya Oura; Yuki Oyama; Hiroaki Kitakaze; Taichi Noda; Norikazu Yabuta; Haruhiko Miyata; Masahito Ikawa, Journal of cell science, The Company of Biologists, 137, (16) , 2024 08 15 |
| Development of functional spermatozoa in mammalian spermiogenesis., Haruhiko Miyata; Keisuke Shimada; Yuki Kaneda; Masahito Ikawa, Development (Cambridge, England), The Company of Biologists, 151, (14) , 2024 07 15 |
| Individual disruption of 12 testis-enriched genes via the CRISPR/Cas9 system does not affect the fertility of male mice., Akira Suzuki; Norikazu Yabuta; Keisuke Shimada; Daisuke Mashiko; Keizo Tokuhiro; Yuki Oyama; Haruhiko Miyata; Thomas X Garcia; Martin M Matzuk; Masahito Ikawa, Journal of reproductive immunology, 163, 104252 - 104252, 2024 06 |
| Gene-deficient mouse model established by CRISPR/Cas9 system reveals 15 reproductive organ-enriched genes dispensable for male fertility., Tuyen Thi Thanh Nguyen; Keizo Tokuhiro; Keisuke Shimada; Haoting Wang; Daisuke Mashiko; Shingo Tonai; Daiji Kiyozumi; Masahito Ikawa, Frontiers in cell and developmental biology, 12, 1411162 - 1411162, 2024 05 01 |
| Affinity-tagged SMAD1 and SMAD5 mouse lines reveal transcriptional reprogramming mechanisms during early pregnancy., Zian Liao; Suni Tang; Kaori Nozawa; Keisuke Shimada; Masahito Ikawa; Diana Monsivais; Martin Matzuk, eLife, 12, 2024 03 27 |
| Disruption of testis-enriched cytochrome c oxidase subunit COX6B2 but not COX8C leads to subfertility., Keisuke Shimada; Yonggang Lu; Masahito Ikawa, Experimental animals, Japanese Association for Laboratory Animal Science, 73, (1) 1 - 10, 2024 02 14 |
| Multiple ageing effects on testicular/epididymal germ cells lead to decreased male fertility in mice., Tsutomu Endo; Kiyonori Kobayashi; Takafumi Matsumura; Chihiro Emori; Manabu Ozawa; Shimpei Kawamoto; Daisuke Okuzaki; Keisuke Shimada; Haruhiko Miyata; Kentaro Shimada; Mayo Kodani; Yu Ishikawa-Yamauchi; Daisuke Motooka; Eiji Hara; Masahito Ikawa, Communications biology, 7, (1) 16 - 16, 2024 01 04 |
| CCDC183 is essential for cytoplasmic invagination around the flagellum during spermiogenesis and male fertility., Keisuke Shimada; Masahito Ikawa, Development (Cambridge, England), 150, (21) , 2023 11 01 |
| Testis-specific proteins, TSNAXIP1 and 1700010I14RIK, are important for sperm motility and male fertility in mice., Yuki Kaneda; Haruhiko Miyata; Keisuke Shimada; Seiya Oura; Masahito Ikawa, Andrology, 11, (5) 799 - 807, 2023 07 |
| TSKS localizes to nuage in spermatids and regulates cytoplasmic elimination during spermiation., Keisuke Shimada; Soojin Park; Seiya Oura; Taichi Noda; Akane Morohoshi; Martin M Matzuk; Masahito Ikawa, Proceedings of the National Academy of Sciences of the United States of America, 120, (11) , 2023 03 14 |
| Evaluation of the efficacy of creatine chemical exchange saturation transfer imaging in assessing testicular maturity., Sohei Kuribayashi; Shinichiro Fukuhara; Go Tsujimura; Takahiro Imanaka; Koichi Okada; Norichika Ueda; Kentaro Takezawa; Hiroshi Kiuchi; Shigeyoshi Saito; Yusuke Takahashi; Hidetaka Kioka; Seiya Oura; Keisuke Shimada; Masahito Ikawa; Norio Nonomura, Reproductive medicine and biology, 22, (1) , 2023 02 |
| IRGC1, a testis-enriched immunity related GTPase, is important for fibrous sheath integrity and sperm motility in mice., Yuki Kaneda; Haruhiko Miyata; Keisuke Shimada; Yuki Oyama; Rie Iida-Norita; Masahito Ikawa, Developmental biology, Elsevier BV, 488, 104 - 113, 2022 08 01 |
| Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis., Shintaro Mise; Akinobu Matsumoto; Keisuke Shimada; Toshiaki Hosaka; Masatomo Takahashi; Kazuya Ichihara; Hideyuki Shimizu; Chisa Shiraishi; Daisuke Saito; Mikita Suyama; Tomoharu Yasuda; Toru Ide; Yoshihiro Izumi; Takeshi Bamba; Tomomi Kimura-Someya; Mikako Shirouzu; Haruhiko Miyata; Masahito Ikawa; Keiichi I Nakayama, Nature communications, 13, (1) 1071 - 1071, 2022 02 28 |
| TULP2 deletion mice exhibit abnormal outer dense fiber structure and male infertility., Yuki Oyama; Haruhiko Miyata; Keisuke Shimada; Tamara Larasati; Yoshitaka Fujihara; Masahito Ikawa, Reproductive medicine and biology, Wiley, 21, (1) , 2022 05 01 |
| LRRC23 is a conserved component of the radial spoke that is necessary for sperm motility and male fertility in mice, Xin Zhang; Jiang Sun; Yonggang Lu; Jintao Zhang; Keisuke Shimada; Taichi Noda; Shuqin Zhao; Takayuki Koyano; Makoto Matsuyama; Shushu Zhou; Jiayan Wu; Masahito Ikawa; Mingxi Liu, Journal of Cell Science, The Company of Biologists, 134, (20) , 2021 09 29 |
| FAM209 associates with DPY19L2 and is required for sperm acrosome biogenesis and fertility in mice, Julio M. Castaneda; Keisuke Shimada; Yuhkoh Satouh; Zhifeng Yu; Masahito Ikawa; Martin M. Matzuk, Journal of Cell Science, The Company of Biologists, 134, (21) , 2021 09 02 |
| SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice, Haruhiko Miyata; Seiya Oura; Akane Morohoshi; Keisuke Shimada; Daisuke Mashiko; Yuki Oyama; Yuki Kaneda; Takafumi Matsumura; Ferheen Abbasi; Masahito Ikawa, Proceedings of the National Academy of Sciences of the United States of America, 118, (35) , 2021 08 31 |
| CRISPR/Cas9-mediated genome editing reveals 12 testis-enriched genes dispensable for male fertility in mice., Yuki Oyama; Haruhiko Miyata; Keisuke Shimada; Yoshitaka Fujihara; Keizo Tokuhiro; Thomas X Garcia; Martin M Matzuk; Masahito Ikawa, Asian journal of andrology, 24, (3) 266 - 272, 2021 07 16 |
| Endometrial receptivity and implantation require uterine BMP signaling through an ACVR2A-SMAD1/SMAD5 axis, Diana Monsivais; Takashi Nagashima; Renata Prunskaite-Hyyryläinen; Kaori Nozawa; Keisuke Shimada; Suni Tang; Clark Hamor; Julio E. Agno; Fengju Chen; Ramya P. Masand; Steven L. Young; Chad J. Creighton; Francesco J. DeMayo; Masahito Ikawa; Se Jin Lee; Martin M. Matzuk, Nature Communications, 12, (1) , 2021 06 07 |
| ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility, Keisuke Shimada; Soojin Park; Haruhiko Miyata; Zhifeng Yu; Akane Morohoshi; Seiya Oura; Martin M. Matzuk; Masahito Ikawa, Proceedings of the National Academy of Sciences of the United States of America, 118, (6) , 2021 02 09 |
| Bi-allelic DNAH8 Variants Lead to Multiple Morphological Abnormalities of the Sperm Flagella and Primary Male Infertility, Chunyu Liu; Haruhiko Miyata; Yang Gao; Yanwei Sha; Shuyan Tang; Zoulan Xu; Marjorie Whitfield; Catherine Patrat; Huan Wu; Emmanuel Dulioust; Shixiong Tian; Keisuke Shimada; Jiangshan Cong; Taichi Noda; Hang Li; Akane Morohoshi; Caroline Cazin; Zine Eddine Kherraf; Christophe Arnoult; Li Jin; Xiaojin He; Pierre F. Ray; Yunxia Cao; Aminata Touré; Feng Zhang; Masahito Ikawa, American Journal of Human Genetics, 107, (2) 330 - 341, 2020 08 06 |
| Tmprss12 is required for sperm motility and uterotubal junction migration in mice, Tamara Larasati; Taichi Noda; Yoshitaka Fujihara; Keisuke Shimada; Tomohiro Tobita; Zhifeng Yu; Martin M. Matzuk; Masahito Ikawa, Biology of Reproduction, 103, (2) 254 - 263, 2020 08 01 |
| CRISPR/Cas9-mediated genome-edited mice reveal 10 testis-enriched genes are dispensable for male fecundity, Soojin Park; Keisuke Shimada; Yoshitaka Fujihara; Zoulan Xu; Kentaro Shimada; Tamara Larasati; Putri Pratiwi; Ryan M. Matzuk; Darius J. Devlin; Zhifeng Yu; Thomas X. Garcia; Martin M. Matzuk; Masahito Ikawa, Biology of Reproduction, 103, (2) 195 - 204, 2020 08 01 |
| PHF7 Modulates BRDT Stability and Histone-to-Protamine Exchange during Spermiogenesis, Chang Rok Kim; Taichi Noda; Hyunkyung Kim; Gibeom Kim; Seongwan Park; Yongwoo Na; Seiya Oura; Keisuke Shimada; Injin Bang; Jun Yeong Ahn; Yong Ryoul Kim; Se Kyu Oh; Hee Jung Choi; Jong Seo Kim; Inkyung Jung; Ho Lee; Yuki Okada; Masahito Ikawa; Sung Hee Baek, Cell Reports, Elsevier {BV}, 32, (4) 107950 - 107950, 2020 07 28 |
| Welfare Impact of Carbon Dioxide Euthanasia on Laboratory Mice and Rats: A Systematic Review, Patricia V. Turner; Debra L. Hickman; Judith van Luijk; Merel Ritskes-Hoitinga; Jan M. Sargeant; T. Miki Kurosawa; Takashi Agui; Vera Baumans; Woo Sung Choi; Yang Kyu Choi; Paul A. Flecknell; Byeong H. Lee; Pedro J. Otaegui; Kathleen R. Pritchett-Corning; Keisuke Shimada, Frontiers in Veterinary Science, 7, 2020 07 22 |
| Testis-enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa, Haruhiko Miyata; Keisuke Shimada; Akane Morohoshi; Seiya Oura; Takafumi Matsumura; Zoulan Xu; Yuki Oyama; Masahito Ikawa, FASEB Journal, 34, (4) 5389 - 5400, 2020 04 |
| Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice, Akane Morohoshi; Haruhiko Miyata; Keisuke Shimada; Kaori Nozawa; Takafumi Matsumura; Ryuji Yanase; Kogiku Shiba; Kazuo Inaba; Masahito Ikawa, PLoS Genetics, Public Library of Science ({PLoS}), 16, (1) , 2020 |
| CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched genes dispensable for male fertility in mice, Yonggang Lu; Seiya Oura; Takafumi Matsumura; Asami Oji; Nobuyuki Sakurai; Yoshitaka Fujihara; Keisuke Shimada; Haruhiko Miyata; Tomohiro Tobita; Taichi Noda; Julio M. Castaneda; Daiji Kiyozumi; Qian Zhang; Tamara Larasati; Samantha A.M. Young; Mayo Kodani; Caitlin A. Huddleston; Matthew J. Robertson; Cristian Coarfa; Ayako Isotani; R. John Aitken; Masaru Okabe; Martin M. Matzuk; Thomas X. Garcia; Masahito Ikawa, Biology of Reproduction, Oxford University Press ({OUP}), 101, (2) 501 - 511, 2019 08 01 |
| Glycerol kinase 2 is essential for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during mouse spermatogenesis, Keisuke Shimada; Hirotaka Kato; Haruhiko Miyata; Masahito Ikawa, Journal of Reproduction and Development, 65, (2) 155 - 162, 2019 |
| Chimeric analysis with newly established EGFP/DsRed2-tagged ES cells identify HYDIN as essential for spermiogenesis in mice, Seiya Oura; Haruhiko Miyata; Taichi Noda; Keisuke Shimada; Takafumi Matsumura; Akane Morohoshi; Ayako Isotani; Masahito Ikawa, Experimental Animals, 68, (1) 25 - 34, 2019 |
| RSPH6A is required for sperm flagellum formation and male fertility in mice, Ferheen Abbasi; Haruhiko Miyata; Keisuke Shimada; Akane Morohoshi; Kaori Nozawa; Takafumi Matsumura; Zoulan Xu; Putri Pratiwi; Masahito Ikawa, Journal of Cell Science, 131, (19) , 2018 10 01 |
| Prolonged rest period enables the detection of micronucleated hepatocytes in the liver of young adult rats after a single dose of diethylnitrosamine or mitomycin C, Keisuke Shimada; Mika Yamamoto; Miyuki Takashima; Jiro Seki; Yoichi Miyamae; Akihiro Wakata, Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 791, 38 - 41, 2015 09 01 |
| Repeated-dose liver micronucleus assay of mitomycin C in young adult rats, Keisuke Shimada; Mika Yamamoto; Miyuki Takashima; Yumi Wako; Kazufumi Kawasako; Yoshinobu Aoki; Jiro Seki; Yoichi Miyamae; Akihiro Wakata, Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 780-781, 85 - 89, 2015 03 01 |
| Evaluation of the repeated-dose liver and gastrointestinal tract micronucleus assays with 22 chemicals using young adult rats: Summary of the collaborative study by the Collaborative Study Group for the Micronucleus Test (CSGMT)/The Japanese Environmental Mutagen Society (JEMS) - Mammalian Mutagenicity Study Group (MMS), Shuichi Hamada; Wakako Ohyama; Rie Takashima; Keisuke Shimada; Kazumi Matsumoto; Satoru Kawakami; Fuyumi Uno; Hajime Sui; Yasushi Shimada; Tadashi Imamura; Shoji Matsumura; Hisakazu Sanada; Kenji Inoue; Shigeharu Muto; Izumi Ogawa; Aya Hayashi; Tomomi Takayanagi; Yosuke Ogiwara; Akihisa Maeda; Emiko Okada; Yukari Terashima; Hironao Takasawa; Kazunori Narumi; Yumi Wako; Kazufumi Kawasako; Masaki Sano; Nobuyuki Ohashi; Takeshi Morita; Hajime Kojima; Masamitsu Honma; Makoto Hayashi, Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 780-781, 2 - 17, 2015 03 01 |
Conference Activities & Talks
| TSKSは精子細胞内のnuageに局在し、細胞質内内容物の除去に関わることで流線型の精子形成を可能にする, 嶋田圭祐, 日本アンドロロジー学会 第44回学術大会 |
| Molecular Mechanism Analysis Involved in Mitochondrial Sheath Formation, Keisuke Shimada, ASA/ICA 2025 Annual Meeting |
| CCDC183 is Essential for Axonemal Compartmentalization during Spermiogenesis, Keisuke Shimada and Masahito Ikawa, SSR Annual meeting 2024 |
| CCDC183は精子形成過程において軸糸微小管のコンパートメント化に必須である, 嶋田圭祐; 伊川正人, 日本アンドロロジー学会 第43回学術大会 |
| CCDC183は精子形成過程における鞭毛周囲の細胞質陥入および軸糸微小管のコンパートメント化に必須である, 嶋田圭祐, 第71回日本実験動物学会総会 |
| 遺伝子改変マウスを用いた精子形成に関する分子メカニズム解析, 嶋田 圭祐, 実験動物札幌セミナー2023 |
| Molecular mechanism analysis involved in mitochondrial sheath formation and spermiation processes using gene-manipulated mice, 嶋田 圭祐, IMP seminar at research Institute of Molecular Pathology |
| TSKS-derived nuages regulate the transportation of cytoplasmic contents during spermiation, Shimada Keisuke; Park Soojin; Oura Seiya; Noda Taichi; Morohoshi Akane; Matzuk M. Martin; Ikawa Masahito, European testis workshop 2023 |
| TSKSは精子細胞内の細胞質内内容物の輸送を制御することで流線型の精子形成を可能にする, 嶋田 圭祐; Park Soojin; 大浦聖矢; 野田大地; 諸星茜; Martin M. Matzuk; 伊川 正人, 第70回日本実験動物学会総会 |
| ARMC12はTBC1D21と協働してミトコンドリア鞘形成を可能にする, 嶋田 圭祐; Park Soojin; 宮田治彦; Zhifeng Yu; 諸星茜; 大浦聖矢; Martin M. Matzuk; 伊川 正人, 第69回日本実験動物学会総会 |
| ARMC12は精子形成期におけるミトコンドリア鞘形成に必須である, 嶋田圭祐, Soojin Park, 宮田治彦, Zhifeng Yu, 諸星茜, 大浦聖矢, Martin M. Matzuk, 伊川正人, 第163回日本獣医学会学術集会 |
| 遺伝子改変マウスを用いた精子鞭毛形成の解析, 諸星 茜、宮田 治彦、嶋田 圭祐、野澤 香織、松村 貴史、梁瀬 隆二、柴 小菊、稲葉 一男、伊川 正人, 新学術領域研究「全能性プログラム：デコーディングからデザインへ」若手勉強会2019 |
| Molecular mechanism of mitochondrial sheath formation revealed through gene-edited mice, Keisuke Shimada, Haruhiko Miyata, Masahito Ikawa, 第14回研究所ネットワーク国際シンポジウム |
| SMDR1は精子形成期においてミトコンドリア間を繋ぎ止めて精子中片部にミトコンドリア鞘を形成するのに必須のタンパク質である, 嶋田圭祐,宮田治彦,伊川正人, 第162回日本獣医学会学術集会 |
| Glycerol kinase 2 is important for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath, Keisuke Shimada, Haruhiko Miyata, Masahito Ikawa, SSR 52nd Annual Meeting |
| マウスRSPH6Aは精子鞭毛の形成と生殖能力に必須である, 宮田 治彦, Ferheen Abbasi, 嶋田 圭祐, 諸星 茜, 野澤 香織, 松村 貴史, Zoulan Xu, Putri Pratiwi, 伊川 正人, 日本アンドロロジー学会第38回学術大会 |
| SMDR1は精子ミトコンドリアの鞭毛への巻き付きに必須である, 嶋田 圭祐, 宮田 治彦, 伊川 正人, 第33回日本生殖免疫学会 |
| マウスDRC7は精子鞭毛形成および生殖能力に必須である, 諸星 茜、宮田 治彦、嶋田 圭祐、野澤 香織、松村 貴史、伊川 正人, 第41回日本分子生物学会年会 |
| SMDR1は精子形成期における精子ミトコンドリアの鞭毛への巻き付きに関わることでミトコンドリア鞘の形成に必須となるタンパク質である, 嶋田 圭祐, 宮田 治彦, 伊川 正人, 第161回日本獣医学会学術集会 |
| 新規EGFP/DsRed2標識ES細胞を用いたゲノム編集とキメラマウスにおける表現型解析, 大浦 聖矢、宮田 治彦、野田 大地、嶋田 圭祐、松村 貴史、諸星 茜、磯谷 綾子、伊川 正人, 若手支援技術講習会2018 |
| RSPH6A is essential for sperm flagellum formation and male fertility in mice, Haruhiko Miyata, Ferheen Abbasi, Keisuke Shimada, Akane Morohoshi, Kaori Nozawa, Takafumi Matsumura, Masahito Ikawa, 51st Annual Meeting of the Society for the Study of Reproduction |
| マウスRSPH6Aは精子鞭毛の形成と生殖能力に必須である, 宮田治彦, Ferheen Abbasi, 嶋田圭祐, 松村貴史, 諸星茜, 伊川正人, 第65回日本実験動物学会総会 |
| Smdr1 is required for sperm mitochondrial alignment during spermatogenesis, 嶋田圭祐,宮田治彦,伊川正人, The 1st International Mitochondria Meeting for Young Scientists |
| Gk2 knockout mice show impaired sperm motility and male infertility, Keisuke Shimada, Hirotaka Kato, Haruhiko Miyata, Yuhkoh Satouh, Masahito Ikawa, 50th Annual Meeting of the Society for the Study of Reproduction |
| Gk2 knockout mice show impaired sperm motility and male infertility, Keisuke Shimada, Hirotaka Kato, Haruhiko Miyata, Yuhkoh Satouh, Masahito Ikawa, Fertilization & Activation of Development (Gordon Research Conference) |
| CRISPR/Cas9システムによるES細胞のゲノム編集とキメラマウス解析, 大字 亜沙美 ,野田 大地 ,嶋田 圭祐 ,伊川 正人, 平成28年度新学術領域研究「学術研究支援基盤形成【先端モデル動物支援プラットフォーム】」若手支援技術講習会 |
Awards & Honors
| 2025 03 , International Society of Andrology, ISA Young Andrologist Award |
| 2024 06 , Japan society of androrology, 令和6年度 日本アンドロロジー学会学術奨励賞 |
| 2024 01 , 杉山記念財団, 第5回 SMF論文表彰制度 優秀賞 |
| 2022 05 , 公益社団法人 日本実験動物学会, 第69回日本実験動物学会総会 優秀発表賞 |
| 2019 01 , Research Institute for Microbial Diseases, Osaka University, Biken Best Academic Award |
Research Grants & Projects
| 日本学術振興会, 科学研究費助成事業 基盤研究(C), 精子形成過程に出現する球状ミトコンドリアの形成メカニズムに迫る, 20230401, 20260331, 嶋田 圭祐 |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists, Analysis of mitochondrial sheath formation mechanism using KO mice, 202004, 202303, 嶋田 圭祐, 本研究ではSmdr3及びSmdr5遺伝子を欠損させたKOマウスの表現型を解析し，これら遺伝子がどのようにミトコンドリア鞘形成に関与しているのかを明らかにすることを目的としている。
SMDR3に対する抗体を作成し，免疫電子顕微鏡観察をしたところ，SMDR3は予想に反してミトコンドリアとは独立した2種類のnuageに局在していることが明らかになった。このnuageはpiRNAが局在するIntermitochondrial cementとは異なるnuageであり，これまでにその機能については全く解析されてこなかったものであった。本研究によりSMDR3に由来するnuageが精子ミトコンドリアの整列に関連することが明らかになった。SMDR3を培養細胞に過剰発現させると，SMDR3は単独で細胞質内にdropletを形成することから，SMDR3は細胞内に液-液相分離を引き起こすことで機能していることも明らかになった。
Smdr5 KOマウスは精子形成過程で精子ミトコンドリアの内部構造に異常を呈し，ミトコンドリア鞘形成がうまくいかないことで雄性不妊となる。今回Smdr5を生殖細胞のみで発現するトランスジェニック（Tg）マウスを作成し、Smdr5 KOマウスに導入すると、雄性不妊がレスキューできることが明らかになった。さらにユビキタスに発現するSmdr5のファミリー遺伝子についても、生殖細胞のみで発現するTgマウスを作成し、Smdr5 KOマウスに導入しても雄性不妊はレスキューできたため，SMDR5の機能はそのファミリータンパク質と同様の働きを有していることが考えられた。したがって，これら2つのタンパク質は発現場所のみ違いがあるものの，機能は同じタンパク質であることが示唆された。 |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B), Revealing the mechanism of mitochondrial localization in the sperm midpiece, 201704, 202003, Keisuke Shimada, We discovered that absence of Sperm mitochondria related gene 1(SMDR1) disrupts mitochondrial sheath formation, resulting in reduced sperm motility and male sterility. In vitro analysis demonstrates that SMDR1 induces mitochondrial clustering, revealing that SMDR1 functions as an adherence factor between mitochondria. In mouse testicular germ cells, SMDR1 localizes to the mitochondrial surface and directly interacts with mitochondrial outer membrane proteins VDAC2 and VDAC3 as well as SMDR2. These findings suggest that SMDR1 binds to VDAC proteins to tether adjacent mitochondria, and this tethering allows mitochondria to properly elongate and coil around the sperm flagellum. Thus, our studies demonstrate for the first time that SMDR1 regulates sperm mitochondrial dynamics, an essential function for mitochondrial sheath formation and male fertility. |
| 公益財団法人千里ライフサイエンス振興財団, 岸本基金研究助成, 精子形成期後半に発生する 2 つの異なる nuage の機能と役割の解明 |
| 公益財団法人 武田科学振興財団, 医学系研究助成（基礎）, Elucidation of molecular mechanisms involved in sperm mitochondrial dynamics |
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