School of Veterinary Medicine, Department of Veterinary Medicine
秋庭正人 アキバ マサト
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Name
AKIBA Masato
Affiliation (Master)
School of Veterinary Medicine, Department of Veterinary Medicine
Affiliation & Job
Rakuno Gakuen University Veterinary Bacteriology, Department of Pathobiology, School of Veterinary Medicine Professor
Degree
Doctor of Veterinary Science, The University of Tokyo
Association Memberships
動物用抗菌剤研究会 日本カンピロバクター研究会 腸管出血性大腸菌感染症研究会 JAPANESE SOCIETY FOR BACTERIOLOGY JAPANESE SOCIETY OF VETERINARY SCIENCE
Research Activities
Research Areas
Life sciences, Veterinary medicine Life sciences, Hygiene and public health (laboratory) Life sciences, Bacteriology
Research Interests
animal, infectious disease, bacteria, molecular epidemiology, pathogenesis, antimicrobial resistance,
Published Papers
Characteristics of antimicrobial residues in manure composts from swine farms: Residual patterns, removal efficiencies, and relation to purchased quantities and composting methods in Japan, Mafumi Watanabe; Prasun Goswami; Katsumasa Kure; Itsuro Yamane; Sota Kobayashi; Masato Akiba; Keerthi S. Guruge, Journal of Hazardous Materials, 2023 08 Nationwide analysis of antimicrobial resistance in pathogenic Escherichia coli strains isolated from diseased swine over 29 years in Japan, Masahiro Kusumoto; Yukino Tamamura-Andoh; Yuna Hikoda-Kogiku; Asami Magome; Erina Okuhama; Keisuke Sato; Yoshino Mizuno; Nobuo Arai; Ayako Watanabe-Yanai; Taketoshi Iwata; Yoshitoshi Ogura; Yasuhiro Gotoh; Keiji Nakamura; Tetsuya Hayashi; Masato Akiba, Frontiers in Microbiology, Frontiers Media SA, 14, 2023 03 17 Occurrence and the potential ecological risk of veterinary antimicrobials in swine farm wastewaters in Japan: Seasonal changes, relation to purchased quantity and after termination of oxytetracycline usage, Mafumi Watanabe; Keerthi S Guruge; Ryuichi Uegaki; Katsumasa Kure; Itsuro Yamane; Sota Kobayashi; Masato Akiba, Environment International, 173, 107812 - 107812, 2023 03 Tryptanthrin Reduces Campylobacter jejuni Colonization in the Chicken Gut by a Bactericidal Mechanism., Taketoshi Iwata; Ayako Watanabe-Yanai; Yukino Tamamura-Andoh; Nobuo Arai; Masato Akiba; Masahiro Kusumoto, Applied and environmental microbiology, 89, (2) , 2023 02 28 Duplication of blaCTX-M-1 and a class 1 integron on the chromosome enhances antimicrobial resistance in Escherichia coli isolated from racehorses in Japan., Yukino Tamamura-Andoh; Hideaki Niwa; Yuta Kinoshita; Eri Uchida-Fujii; Nobuo Arai; Ayako Watanabe-Yanai; Taketoshi Iwata; Masato Akiba; Masahiro Kusumoto, Journal of global antimicrobial resistance, 27, 225 - 227, 2021 12 The association between antimicrobials and the antimicrobial-resistant phenotypes and resistance genes of Escherichia coli isolated from hospital wastewaters and adjacent surface waters in Sri Lanka, Keerthi S. Guruge; Yukino A. Tamamura; Prasun Goswami; Rumi Tanoue; K.B.S.N. Jinadasa; Kei Nomiyama; Takeshi Ohura; Tatsuya Kunisue; Shinsuke Tanabe; Masato Akiba, Chemosphere, Elsevier BV, 279, 130591 - 130591, 2021 09 Identification of a Recently Dominant Sublineage in Salmonella 4,[5],12:i:- Sequence Type 34 Isolated From Food Animals in Japan, Nobuo Arai; Tsuyoshi Sekizuka; Yukino Tamamura-Andoh; Lisa Barco; Atsushi Hinenoya; Shinji Yamasaki; Taketoshi Iwata; Ayako Watanabe-Yanai; Makoto Kuroda; Masato Akiba; Masahiro Kusumoto, Frontiers in Microbiology, Frontiers Media SA, 12, 2021 07 01 A survey of antimicrobial resistance in Escherichia coli isolated from wild sika deer (Cervus nippon) in Japan, Yukino TAMAMURA-ANDOH; Nobuyuki TANAKA; Keisuke SATO; Yoshino MIZUNO; Nobuo ARAI; Ayako WATANABE-YANAI; Masato AKIBA; Masahiro KUSUMOTO, Journal of Veterinary Medical Science, Japanese Society of Veterinary Science, 83, (5) 754 - 758, 2021 Influence of SOS-inducing agents on the expression of ArtAB toxin gene in Salmonella enterica and Salmonella bongori., Shou Miura; Yukino Tamamura; Mariko Takayasu; Miwa Sasaki; Natsuko Nishimura; Kanetaka Tokugawa; Izumi Suwa; Ryo Murata; Masato Akiba; Masahiro Kusumoto; Ikuo Uchida, Microbiology (Reading, England), 166, (8) 785 - 793, 2020 08 Occurrence of antimicrobial-resistant Escherichia coli in sewage treatment plants of South India, Praveenkumarreddy Yerabham; Masato Akiba; Keerthi Siri Guruge; Keshava Balakrishna; Kalwaje Eshwara Vandana; Virendra Kumar, JOURNAL OF WATER SANITATION AND HYGIENE FOR DEVELOPMENT, IWA PUBLISHING, 10, (1) 48 - 55, 2020 03 Molecular Epidemiology of <i>Campylobacter fetus</i> Isolates from Cattle in the Tokachi Area of Hokkaido, Japan, Hiroyasu TAKAHASHI; Taketoshi IWATA; Kiyoko NOBUMOTO; Erika OKAMOTO; Masato AKIBA; Satoshi TACHIBANA, Journal of the Japan Veterinary Medical Association, Japan Veterinary Medical Association, 72, (12) 750 - 756, 2019 12 20 Identification of an Antigen Specific to a Protective Salmonella Monoclonal Antibody, Swarmistha Devi ARIBAM; Yumiko MASUKAGAMI; Kazumasa SHIRAIWA; Sayaka NISHIKAWA; Yohsuke OGAWA; Masato AKIBA; Yoshihiro SHIMOJI; Masahiro EGUCHI, JARQ, 53, (3) , 2019 07 Salmonella Genomic Island 3 Is an Integrative and Conjugative Element and Contributes to Copper and Arsenic Tolerance of Salmonella enterica, Nobuo Arai; Tsuyoshi Sekizuka; Yukino Tamamura; Masahiro Kusumoto; Atsushi Hinenoya; Shinji Yamasaki; Taketoshi Iwata; Ayako Watanabe-Yanai; Makoto Kuroda; Masato Akiba, Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 63, (9) , 2019 06 17 Transcriptomic analysis of Campylobacter jejuni grown in a medium containing serine as the main energy source, Ayako Watanabe-YanaiTaketoshi IwataMasahiro KusumotoYukino TamamuraMasato Akiba, Archives of Microbiology, 2018 11 In vivo effect of a TLR5 SNP (C1205T) on Salmonella enterica serovar Typhimurium infection in weaned, specific pathogen-free Landrace piglets, Yoshihiro Muneta; Nobuo Arai; Yoko Yakabe; Masahiro Eguchi; Tomoyuki Shibahara; Akiko Sakuma; Hiroki Shinkai; Hirohide Uenishi; Kensuke Hirose; Masato Akiba, Microbiology and Immunology, Wiley, 62, (6) 380 - 387, 2018 06 Phylogenetic Characterization ofSalmonella entericaSerovar Typhimurium and Its Monophasic Variant Isolated from Food Animals in Japan Revealed Replacement of Major Epidemic Clones in the Last 4 Decades, Nobuo Arai; Tsuyoshi Sekizuka; Yukino Tamamura; Kiyoshi Tanaka; Lisa Barco; Hidemasa Izumiya; Masahiro Kusumoto; Atsushi Hinenoya; Shinji Yamasaki; Taketoshi Iwata; Ayako Watanabe; Makoto Kuroda; Ikuo Uchida; Masato Akiba, Journal of Clinical Microbiology, American Society for Microbiology, 56, (5) , 2018 02 28 Seasonal movement of trace-element discharge in a typical south-Indian suburban community, Derrick Ian Joshua; Sumith Abeykoon; Izumi Watanabe; Lucyna Paszek; Keshava Balakrishna; Masato Akiba; Keerthi Siri Guruge, Water Science and Technology, IWA Publishing, 77, (4) 1035 - 1047, 2018 02 01 Population structure of Escherichia coli O26 : H11 with recent and repeated stx2 acquisition in multiple lineages., Yoshitoshi Ogura; Yasuhiro Gotoh; Takehiko Itoh; Mitsuhiko P Sato; Kazuko Seto; Shyuji Yoshino; Junko Isobe; Yoshiki Etoh; Mariko Kurogi; Keiko Kimata; Eriko Maeda; Denis Piérard; Masahiro Kusumoto; Masato Akiba; Kiyoshi Tominaga; Yumi Kirino; Yuki Kato; Katsuhiko Shirahige; Tadasuke Ooka; Nozomi Ishijima; Ken-Ichi Lee; Sunao Iyoda; Jacques Georges Mainil; Tetsuya Hayashi, Microbial genomics, 3, (11) , 2017 11 Nationwide investigation of Shiga toxin-producing Escherichia coli among cattle in Japan revealed the risk factors and potentially virulent subgroups, K. Lee; M. Kusumoto; T. Iwata; S. Iyoda; M. Akiba, EPIDEMIOLOGY AND INFECTION, CAMBRIDGE UNIV PRESS, 145, (8) 1557 - 1566, 2017 06 Determination of O:4 antigen-antibody affinity level in O: 5 antigen positive and negative variants of Salmonella enterica serovar Typhimurium, Yuka Nakai; Akihisa Ito; Yohsuke Ogawa; Swarmistha Devi Aribam; Marta Elsheimer-Matulova; Kazumasa Shiraiwa; Stevens M. B. Kisaka; Hirokazu Hikono; Sayaka Nishikawa; Masato Akiba; Kazuyoshi Kawahara; Yoshihiro Shimoji; Masahiro Eguchi, FEMS MICROBIOLOGY LETTERS, OXFORD UNIV PRESS, 364, (7) , 2017 04 Trace element distribution and risk assessment in South Indian surface waterways, K. S. Guruge; P. Goswami; I. Watanabe; S. Abeykoon; V. P. Prabhasankar; K. R. Binu; D. I. Joshua; K. Balakrishna; M. Akiba; N. Munuswamy, INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY, SPRINGER, 14, (1) 1 - 18, 2017 01 Transcription factor-mediated induction of gene expression and their association with the prevalence of antimicrobial-resistant Escherichia coli in wastewater, K. S. Guruge; N. Yamanaka; M. Yoshioka; M. Akiba; T. Yamamoto; T. Tsutsui; K. Balakrishna, TOXICOLOGY LETTERS, ELSEVIER IRELAND LTD, 259, S125 - S126, 2016 10 Peptidoglycan Acetylation of Campylobacter jejuni Is Essential for Maintaining Cell Wall Integrity and Colonization in Chicken Intestines, Taketoshi Iwata; Ayako Watanabe; Masahiro Kusumoto; Masato Akiba, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMER SOC MICROBIOLOGY, 82, (20) 6284 - 6290, 2016 10 Spatio-temporal distribution, source, and genotoxic potential of polycyclic aromatic hydrocarbons in estuarine and riverine sediments from southern India, Prasun Goswami; Takeshi Ohura; Keerthi S. Guruge; Miyako Yoshioka; Noriko Yamanaka; Masato Akiba; Natesan Munuswamy, ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, ACADEMIC PRESS INC ELSEVIER SCIENCE, 130, 113 - 123, 2016 08 Colistin-Resistant mcr-1-Positive Pathogenic Escherichia coil in Swine, Japan, 2007-2014, Masahiro Kusumoto; Yoshitoshi Ogura; Yasuhiro Gotoh; Taketoshi Iwata; Tetsuya Hayashi; Masato Akiba, EMERGING INFECTIOUS DISEASES, CENTERS DISEASE CONTROL, 22, (7) 1315 - 1317, 2016 07 Removal rates of antibiotics in four sewage treatment plants in South India, Valiparambil Prabhakaranunni Prabhasankar; Derrick Ian Joshua; Keshava Balakrishna; Iyanee Faroza Siddiqui; Sachi Taniyasu; Nobuyoshi Yamashita; Kurunthachalam Kannan; Masato Akiba; Yerabham Praveenkumarreddy; Keerthi S. Guruge, ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, SPRINGER HEIDELBERG, 23, (9) 8679 - 8685, 2016 05 Distribution and Relationships of Antimicrobial Resistance Determinants among Extended-Spectrum-Cephalosporin-Resistant or Carbapenem-Resistant Escherichia coli Isolates from Rivers and Sewage Treatment Plants in India, Masato Akiba; Tsuyoshi Sekizuka; Akifumi Yamashita; Makoto Kuroda; Yuki Fujii; Misato Murata; Ken-ichi Lee; Derrick Ian Joshua; Keshava Balakrishna; Indira Bairy; Kaushik Subramanian; Padma Krishnan; Natesan Munuswamy; Ravindra K. Sinha; Taketoshi Iwata; Masahiro Kusumoto; Keerthi S. Guruge, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, AMER SOC MICROBIOLOGY, 60, (5) 2972 - 2980, 2016 05 Emergence of a Multidrug-Resistant Shiga Toxin-Producing Enterotoxigenic Escherichia coli Lineage in Diseased Swine in Japan, Masahiro Kusumoto; Yuna Hikoda; Yuki Fujii; Misato Murata; Hirotsugu Miyoshi; Yoshitoshi Ogura; Yasuhiro Gotoh; Taketoshi Iwata; Tetsuya Hayashi; Masato Akiba, JOURNAL OF CLINICAL MICROBIOLOGY, AMER SOC MICROBIOLOGY, 54, (4) 1074 - 1081, 2016 04 Investigation of a plasmid genome database for colistin-resistance gene mcr-1, Satowa Suzuki; Mamoru Ohnishi; Michiko Kawanishi; Masato Akiba; Makoto Kuroda, LANCET INFECTIOUS DISEASES, ELSEVIER SCI LTD, 16, (3) 284 - 285, 2016 03 Source-Related Effects of Wastewater on Transcription Factor (AhR, CAR and PXR)-Mediated Induction of Gene Expression in Cultured Rat Hepatocytes and Their Association with the Prevalence of Antimicrobial-Resistant Escherichia coli, Keerthi S. Guruge; Noriko Yamanaka; Miyuki Sonobe; Wataru Fujizono; Miyako Yoshioka; Masato Akiba; Takehisa Yamamoto; Derrick I. Joshua; Keshava Balakrishna; Nobuyoshi Yamashita; Kurunthachalam Kannan; Toshiyuki Tsutsui, PLOS ONE, PUBLIC LIBRARY SCIENCE, 10, (9) , 2015 09 Lipooligosaccharide Core Truncations Affect the Ability of Campylobacter jejuni to Attach to Glass and Form Biofilms under Aerobic Conditions, Vu Tuan Nguyen; Taketoshi Iwata; Masahiro Kusumoto; Masato Akiba, JARQ-JAPAN AGRICULTURAL RESEARCH QUARTERLY, JAPAN INT RESEARCH CENTER AGRICULTURAL SCIENCES, 49, (3) 269 - 275, 2015 07 Molecular typing of Salmonella enterica serovar 4,[5],12:i:- isolates from humans, animals and river water in Japan by multilocus variable-number tandem repeat analysis and pulsed-field gel electrophoresis, Noriko Ido; Kaori Iwabuchi; Yusuke Sato'o; Yasuo Sato; Masaru Sugawara; Gakuji Yaegashi; Masaru Konno; Masato Akiba; Kiyoshi Tanaka; Katsuhiko Omoe; Ikuo Uchida, JOURNAL OF VETERINARY MEDICAL SCIENCE, JAPAN SOC VET SCI, 77, (5) 609 - 613, 2015 05 Impact of wastewater from different sources on the prevalence of antimicrobial-resistant Escherichia coli in sewage treatment plants in South India, Masato Akiba; Hironobu Senba; Haruna Otagiri; Valipparambil P. Prabhasankar; Sachi Taniyasu; Nobuyoshi Yamashita; Ken-ichi Lee; Takehisa Yamamoto; Toshiyuki Tsutsui; Derrick Ian Joshua; Keshava Balakrishna; Indira Bairy; Taketoshi Iwata; Masahiro Kusumoto; Kurunthachalam Kannan; Keerthi S. Guruge, ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, ACADEMIC PRESS INC ELSEVIER SCIENCE, 115, 203 - 208, 2015 05 Extensive amplification of GI-VII-6, a multidrug resistance genomic island of Salmonella enterica serovar Typhimurium, increases resistance to extended-spectrum cephalosporins, Ken-ichi Lee; Masahiro Kusumoto; Tsuyoshi Sekizuka; Makoto Kuroda; Ikuo Uchida; Taketoshi Iwata; Susumu Okamoto; Kimiko Yabe; Takashi Inaoka; Masato Akiba, FRONTIERS IN MICROBIOLOGY, FRONTIERS RESEARCH FOUNDATION, 6, 2015 02 Monoclonal antibody-based competitive enzyme-linked immunosorbent assay to detect antibodies to O:4 Salmonella in the sera of livestock and poultry, Swarmistha Devi Aribam; Yohsuke Ogawa; Hidenori Matsui; Jiro Hirota; Masashi Okamura; Masato Akiba; Yoshihiro Shimoji; Masahiro Eguchi, JOURNAL OF MICROBIOLOGICAL METHODS, ELSEVIER SCIENCE BV, 108, 1 - 3, 2015 01 Characteristics of Salmonella enterica Serovar 4,[5], 12: i:- as a Monophasic Variant of Serovar Typhimurium, Noriko Ido; Ken-ichi Lee; Kaori Iwabuchi; Hidemasa Izumiya; Ikuo Uchida; Masahiro Kusumoto; Taketoshi Iwata; Makoto Ohnishi; Masato Akiba, PLOS ONE, PUBLIC LIBRARY SCIENCE, 9, (8) , 2014 08 Molecular typing of Salmonella enterica serovar Enteritidis isolates from food-producing animals in Japan by multilocus variable-number tandem repeat analysis: evidence of clonal dissemination and replacement, Ayumi Kobayashi; Sayaka Takahashi; Masaaki Ono; Kiyoshi Tanaka; Masato Kishima; Masato Akiba; Ikuo Uchida, ACTA VETERINARIA SCANDINAVICA, BIOMED CENTRAL LTD, 56, 2014 05 HPCD子豚へのSalmonella enterica serovar Choleraesuis実験感染 病態の再現とTLR5一塩基多型(C1205T)の影響検証の試み, 溝呂木 聖子; 矢ヶ部 陽子; 芝原 友幸; 秋庭 正人; 楠本 正博; 佐久間 晶子; 中條 満; 新開 浩樹; 上西 博英; 宗田 吉広, 日本豚病研究会報, 日本豚病研究会, (63) 6 - 11, 2014 02 Lineage-Specific Distribution of Insertion Sequence Excision Enhancer in Enterotoxigenic Escherichia coli Isolated from Swine, Masahiro Kusumoto; Dai Fukamizu; Yoshitoshi Ogura; Eiji Yoshida; Fumiko Yamamoto; Taketoshi Iwata; Tadasuke Ooka; Masato Akiba; Tetsuya Hayashi, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMER SOC MICROBIOLOGY, 80, (4) 1394 - 1402, 2014 02 Whole-genome sequence of CMY-2 β-lactamase-producing Salmonella enterica serovar Typhimurium strain L-3553, Tsuyoshi Sekizuka; Ken-Ichi Lee; Makoto Kuroda; Masahiro Kusumoto; Taketoshi Iwata; Ikuo Uchida; Kiyoshi Tanaka; Yukino Tamamura; Masato Akiba, Genome Announcements, American Society for Microbiology, 2, (4) , 2014 Complete Nucleotide Sequences of Virulence-Resistance Plasmids Carried by Emerging Multidrug-Resistant Salmonella enterica Serovar Typhimurium Isolated from Cattle in Hokkaido, Japan, Yukino Tamamura; Kiyoshi Tanaka; Masato Akiba; Toru Kanno; Shinichi Hatama; Ryoko Ishihara; Ikuo Uchida, PLoS ONE, 8, (10) , 2013 10 14 Distribution of extended-spectrum cephalosporin resistance determinants in Salmonella enterica and Escherichia coli isolated from broilers in southern Japan, F. Shahada; T. Chuma; G. Kosugi; M. Kusumoto; T. Iwata; M. Akiba, Poultry Science, 92, (6) 1641 - 1649, 2013 06 Genomic Analysis of Salmonella enterica Serovar Typhimurium Definitive Phage Type 104, Hidemasa Izumiya; Jun Terajima; Shouji Yamamoto; Makoto Ohnishi; Haruo Watanabe; Akemi Kai; Takayuki Kurazono; Masumi Taguchi; Tetsuo Asai; Masato Akiba; Yuko Matsumoto; Yutaka Tamura, EMERGING INFECTIOUS DISEASES, CENTERS DISEASE CONTROL, 19, (5) 823 - 825, 2013 05 Chronological change of resistance to beta-lactams in Salmonella enterica serovar Infantis isolated from broilers in Japan, Takehisa Chuma; Daisuke Miyasako; Hesham Dahshan; Tomoko Takayama; Yuko Nakamoto; Francis Shahada; Masato Akiba; Karoku Okamoto, FRONTIERS IN MICROBIOLOGY, FRONTIERS RESEARCH FOUNDATION, 4, 2013 05 Effects of Lipooligosaccharide Inner Core Truncation on Bile Resistance and Chick Colonization by Campylobacter jejuni, Taketoshi Iwata; Kazuhiro Chiku; Ken-ichi Amano; Masahiro Kusumoto; Mayumi Ohnishi-Kameyama; Hiroshi Ono; Masato Akiba, PLoS ONE, 8, (2) , 2013 02 20 Colonization of chicken flocks by Campylobacter jejuni in multiple farms in Japan, S. Yano; T. Kira; Y. Morishita; K. Ishihara; T. Asai; T. Iwata; M. Akiba; T. Murase, Poultry Science, 92, (2) 375 - 381, 2013 02 Distinguishable epidemics of multidrug-resistant Salmonella typhimurium DT104 in different hosts, A. E. Mather; S. W.J. Reid; D. J. Maskell; J. Parkhill; M. C. Fookes; S. R. Harris; D. J. Brown; J. E. Coia; M. R. Mulvey; M. W. Gilmour; L. Petrovska; E. De Pinna; M. Kuroda; M. Akiba; H. Izumiya; T. R. Connor; M. A. Suchard; P. Lemey; D. J. Mellor; D. T. Haydon; N. R. Thomson, Science, American Association for the Advancement of Science, 341, (6153) 1514 - 1517, 2013 Fluoroquinolone resistance mechanisms in an Escherichia coli isolate, HUE1, without quinolone resistance-determining region mutations, Toyotaka Sato; Shin-Ichi Yokota; Ikuo Uchida; Torahiko Okubo; Masaru Usui; Masahiro Kusumoto; Masato Akiba; Nobuhiro Fujii; Yutaka Tamura, Frontiers in Microbiology, Frontiers Research Foundation, 4, (MAY) , 2013 Molecular typing of Salmonella enterica serotype Typhimurium and serotype 4,5,12:i:- isolates from cattle by multiple-locus variable-number tandem-repeats analysis, Atsushi Kurosawa; Takeshi Imamura; Kiyoshi Tanaka; Yukino Tamamura; Ikuo Uchida; Ayumi Kobayashi; Eiji Hata; Toru Kanno; Masato Akiba; Shouichirou Yukawa; Yutaka Tamura, VETERINARY MICROBIOLOGY, ELSEVIER SCIENCE BV, 160, (1-2) 264 - 268, 2012 11 Change in Antimicrobial Resistance Pattern in Salmonella enterica serovar Typhimurium Isolates Detected in a Beef Cattle Farm, Masaru Sugawara; Francis Shahada; Hidemasa Izumiya; Haruo Watanabe; Ikuo Uchida; Yukino Tamamura; Masahiro Kusumoto; Taketoshi Iwata; Masato Akiba, JOURNAL OF VETERINARY MEDICAL SCIENCE, JAPAN SOC VET SCI, 74, (1) 93 - 97, 2012 01 Characterization of Salmonella enterica Serovar Typhimurium Isolates Harboring a Chromosomally Encoded CMY-2 beta-Lactamase Gene Located on a Multidrug Resistance Genomic Island, Francis Shahada; Tsuyoshi Sekizuka; Makoto Kuroda; Masahiro Kusumoto; Daiki Ohishi; Atsuko Matsumoto; Hizuru Okazaki; Kiyoshi Tanaka; Ikuo Uchida; Hidemasa Izumiya; Haruo Watanabe; Yukino Tamamura; Taketoshi Iwata; Masato Akiba, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, AMER SOC MICROBIOLOGY, 55, (9) 4114 - 4121, 2011 09 Porcine Toll-like receptors: Recognition of Salmonella enterica serovar Choleraesuis and influence of polymorphisms, Hiroki Shinkai; Rintaro Suzuki; Masato Akiba; Naohiko Okumura; Hirohide Uenishi, MOLECULAR IMMUNOLOGY, PERGAMON-ELSEVIER SCIENCE LTD, 48, (9-10) 1114 - 1120, 2011 05 Rapid identification of Salmonella enterica serovars, Typhimurium, Choleraesuis, Infantis, Hadar, Enteritidis, Dublin and Gallinarum, by multiplex PCR, Masato Akiba; Masahiro Kusumoto; Taketoshi Iwata, JOURNAL OF MICROBIOLOGICAL METHODS, ELSEVIER SCIENCE BV, 85, (1) 9 - 15, 2011 04 Molecular and Phenotypic Characteristics of CMY-2 beta-Lactamase-Producing Salmonella enterica Serovar Typhimurium Isolated from Cattle in Japan, Masaru Sugawara; Junko Komori; Marimo Kawakami; Hidemasa Izumiya; Haruo Watanabe; Masato Akiba, JOURNAL OF VETERINARY MEDICAL SCIENCE, JAPAN SOC VET SCI, 73, (3) 345 - 349, 2011 03 Molecular Epidemiology of Salmonella enterica Serovar Typhimurium Isolates from Cattle in Hokkaido, Japan: Evidence of Clonal Replacement and Characterization of the Disseminated Clone, Yukino Tamamura; Ikuo Uchida; Kiyoshi Tanaka; Hizuru Okazaki; Satoru Tezuka; Hideki Hanyu; Natsumi Kataoka; Sou-ichi Makino; Masato Kishima; Takayuki Kubota; Toru Kanno; Shinichi Hatama; Ryoko Ishihara; Eiji Hata; Hironari Yamada; Yuuji Nakaoka; Masato Akiba, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMER SOC MICROBIOLOGY, 77, (5) 1739 - 1750, 2011 03 Molecular and Phenotypic Characteristics of Salmonella enterica Serovar 4,5,12:i:- Isolated from Cattle and Humans in Iwate Prefecture, Japan, Noriko Ido; Takashi Kudo; Koji Sasaki; Masato Motokawa; Kaori Iwabuchi; Hiroki Matsudate; Yukio M. Seimiya; Masato Akiba, JOURNAL OF VETERINARY MEDICAL SCIENCE, JAPAN SOC VET SCI, 73, (2) 241 - 244, 2011 02 Insertion sequence-excision enhancer removes transposable elements from bacterial genomes and induces various genomic deletions, Masahiro Kusumoto; Tadasuke Ooka; Yoshiaki Nishiya; Yoshitoshi Ogura; Takashi Saito; Yasuhiko Sekine; Taketoshi Iwata; Masato Akiba; Tetsuya Hayashi, NATURE COMMUNICATIONS, NATURE PUBLISHING GROUP, 2, 2011 01 Identification of a Key Amino Acid of LuxS Involved in AI-2 Production in Campylobacter jejuni, Paul Plummer; Jinge Zhu; Masato Akiba; Dehua Pei; Qijing Zhang, PLOS ONE, PUBLIC LIBRARY SCIENCE, 6, (1) , 2011 01 Characterization of Antibiotic Resistance and the Emergence of AmpC-Producing Salmonella Infantis from Pigs, Hesham Dahshan; Takehisa Chuma; Francis Shahada; Masato Akiba; Hideki Fujimoto; Keishirou Akasaka; Yuji Kamimura; Karoku Okamoto, JOURNAL OF VETERINARY MEDICAL SCIENCE, JAPAN SOC VET SCI, 72, (11) 1437 - 1442, 2010 11 Detection and Characterization of Extended-Spectrum beta-Lactamase (TEM-52)-Producing Salmonella Serotype Infantis from Broilers in Japan, Francis Shahada; Takehisa Chuma; Hesham Dahshan; Masato Akiba; Masuo Sueyoshi; Karoku Okamoto, FOODBORNE PATHOGENS AND DISEASE, MARY ANN LIEBERT INC, 7, (5) 515 - 521, 2010 05 Pulsed-Field Gel Electrophoresis Profile Changes Resulting from Spontaneous Chromosomal Deletions in Enterohemorrhagic Escherichia coli O157:H7 during Passage in Cattle, Noriyo Yoshii; Yoshitoshi Ogura; Tetsuya Hayashi; Takashi Ajiro; Toshiya Sameshima; Muneo Nakazawa; Masahiro Kusumoto; Taketoshi Iwata; Masato Akiba, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMER SOC MICROBIOLOGY, 75, (17) 5719 - 5726, 2009 09 Salmonella enterica serotype Typhimurium DT104 ArtA-dependent modification of pertussis toxin-sensitive G proteins in the presence of [32P]NAD, Ikuo Uchida; Ryoko Ishihara; Kiyoshi Tanaka; Eiji Hata; Sou-Ichi Makino; Toru Kanno; Shinichi Hatama; Masato Kishima; Masato Akiba; Atsushi Watanabe; Takayuki Kubota, Microbiology, 155, (11) 3710 - 3718, 2009 Phage type and antimicrobial susceptibility of Salmonella enterica serovar Enteritidis from food-producing animals in Japan between 1976 and 2004., Tetsuo Asai; Kazuki Harada; Akemi Kojima; Toshiya Sameshima; Toshio Takahashi; Masato Akiba; Muneo Nakazawa; Hidemasa Izumiya; Jun Terajima; Haruo Watanbe, The new microbiologica, 31, (4) 555 - 9, 2008 10 Antimicrobial resistance of Salmonella enterica serovar Typhimurium isolated from cattle in Japan, Masato Akiba; Toshiya Sameshima; Ikuo Uchida; Muneo Nakazawa, Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment, 25, (9) 1076 - 1079, 2008 09 Antimicrobial resistance of Salmonella enterica serovar Typhimurium isolated from cattle in Japan, Masato Akiba; Toshiya Sameshima; Ikuo Uchida; Muneo Nakazawa, FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, TAYLOR & FRANCIS LTD, 25, (9) 1076 - 1079, 2008 Changes in antimicrobial susceptibility in a population of Salmonella enterica serovar Dublin isolated from cattle in Japan from 1976 to 2005, Masato Akiba; Yuji Nakaoka; Muneyoshi Kida; Yukiko Ishioka; Toshiya Sameshima; Noriyo Yoshii; Muneo Nakazawa; Ikuo Uchida; Nobuyuki Terakado, JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, OXFORD UNIV PRESS, 60, (6) 1235 - 1242, 2007 12 Detection and characterization of variant Salmonella genomic island is from Salmonella Derby isolates, Masato Akiba; Kotaro Nakamura; David Shinoda; Noriyo Yoshii; Hiroya Ito; Ikuo Uchida; Muneo Nakazawa, JAPANESE JOURNAL OF INFECTIOUS DISEASES, NATL INST INFECTIOUS DISEASES, 59, (5) 341 - 345, 2006 10 Comparison of PCR-RFLP and PFGE for determining the clonality of enterohemorrhagic Escherichia coli strains, Kensuke Shima; Noriyo Yoshii; Masato Akiba; Kazuhiko Nishimura; Muneo Nakazawa; Shinji Yamasaki, FEMS Microbiology Letters, 257, (1) 124 - 131, 2006 04 Interaction of CmeABC and CmeDEF in conferring antimicrobial resistance and maintaining cell viability in Campylobacter jejuni, M Akiba; J Lin; YW Barton; QJ Zhang, JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, OXFORD UNIV PRESS, 57, (1) 52 - 60, 2006 01 The artAB genes encode a putative ADP-ribosyltransferase toxin homologue associated with Salmonella enterica serovar Typhimurium DT104, Mariko Saitoh; Kiyoshi Tanaka; Kei Nishimori; Sou-Ichi Makino; Toru Kanno; Ryoko Ishihara; Shinichi Hatama; Rie Kitano; Masato Kishima; Toshiya Sameshima; Masato Akiba; Muneo Nakazawa; Yuichi Yokomizo; Ikuo Uchida, Microbiology, 151, (9) 3089 - 3096, 2005 09 Contamination of Salmonella in retail meats and shrimps in the Mekong Delta, Vietnam, Thi Phan Tran; Ly Thi Lien Khai; Natsue Ogasawara; Thu Tam Nguyen; Alexandre Tomomitsu Okatani; Masato Akiba; Hideki Hayashidani, Journal of Food Protection, 68, (5) 1077 - 1080, 2005 05 CmeR functions as a transcriptional repressor for the multidrug efflux pump CmeABC in Campylobacter jejuni, J Lin; M Akiba; O Sahin; QJ Zhang, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, AMER SOC MICROBIOLOGY, 49, (3) 1067 - 1075, 2005 03 The importance of a 95-kb virulence plasmid in the pathogenicity of Salmonella abortusequi in horses, Toru Anzai; Yasushi Kuwamoto; Seiji Hobo; Hidekazu Niwa; Yoshinari Katayama; Hirotaka Ode; Nariaki Abe; Akira Doi; Masato Akiba; Toshiya Sameshima, Journal of Equine Science, 16, (4) 111 - 116, 2005 Prevalence of Salmonella spp. in pigs, chickens and ducks in the Mekong Delta, Vietnam, Thi Phan Tran; Thi Lien Khai Ly; Thu Tan Nguyen; Masato Akiba; Natsue Ogasawara; David Shinoda; Alexandre Tomomitsu Okatani; Hideki Hayashidani, Journal of Veterinary Medical Science, 66, (8) 1011 - 1014, 2004 08 Molecular Characterization of a Prophage of Salmonella enterica Serotype Typhimurium DT104, Kiyoshi Tanaka; Kei Nishimori; Sou-Ichi Makino; Tomoko Nishimori; Toru Kanno; Ryoko Ishihara; Toshiya Sameshima; Masato Akiba; Muneo Nakazawa; Yuichi Yokomizo; Ikuo Uchida, Journal of Clinical Microbiology, 42, (4) 1807 - 1812, 2004 04 Analysis of the complete nucleotide sequence of an Actinobacillus pleuropneumoniae streptomycin-sulfonamide resistance plasmid, pMS260, Hiroya Ito; Hiroshi Ishii; Masato Akiba, Plasmid, Academic Press Inc., 51, (1) 41 - 47, 2004 Virulence and other biological properties of Salmonella Typhimurium isolated from foals in Japan from 1981 until 1996, Toru Anzai; Yasushi Kuwamoto; Ryuichi Wada; Masato Akiba; Tatsuo Ohya; Toshiya Sameshima, Journal of Equine Science, 14, (3) 87 - 92, 2003 09 Comparison of Salmonella enterica serovar Abortusequi isolates of equine origin by pulsed-field gel electrophoresis and fluorescent amplified-fragment length polymorphism fingerprinting, M Akiba; Uchida, I; K Nishimori; K Tanaka; T Anzai; Y Kuwamoto; R Wada; T Ohya; H Ito, VETERINARY MICROBIOLOGY, ELSEVIER SCIENCE BV, 92, (4) 379 - 388, 2003 04 Isolation of Salmonella Strains from the Aquatic Environment and Comparison with those of Animal Origin in Tan Phu Thanh Village, Mekong Delta, Vietnam, Tran Thi Phan; Ly Thi Lien Khai; Chau Ba Loc; Hideki Hayashidani; Toshiya Sameshima; Takeshi Watanabe; Toshiaki Taniguchi; Hideki Kobayashi; Hiroya Ito; Masato Akiba, Japan Agricultural Research Quarterly, Japan International Research Center for Agricultural Sciences, 37, (4) 237 - 241, 2003 Use of a trial probiotic product in calves experimentally infected with Escherichia coli O157, T Ohya; M Akiba; H Ito, JARQ-JAPAN AGRICULTURAL RESEARCH QUARTERLY, TROPICAL AGRICULTURE RES CENTR, 35, (3) 189 - 194, 2001 07 Molecular typing and epidemiological study of Salmonella enterica serotype typhimurium isolates from cattle by fluorescent amplified-fragment length polymorphism fingerprinting and pulsed-field gel electrophoresis, Y Tamada; Y Nakaoka; K Nishimori; A Doi; T Kumaki; N Uemura; K Tanaka; SI Makino; T Sameshima; M Akiba; M Nakazawa; Uchida, I, JOURNAL OF CLINICAL MICROBIOLOGY, AMER SOC MICROBIOLOGY, 39, (3) 1057 - 1066, 2001 03 Salmonella typhimurium DT104 from livestock in Japan, Toshiya Sameshima; Masato Akiba; Hidemasa Izumiya; Jun Terajima; Kazumiti Tamura; Haruo Watanabe; Muneo Nakazawa, Japanese Journal of Infectious Diseases, 53, (1) 15 - 16, 2000 02 A comparison of Escherichia coli O157 isolates from cattle in Japan and the USA by molecular biological methods, M. Akiba; D. H. Rice; M. A. Davis; T. Masuda; T. Sameshima; M. Nakazawa; D. D. Hancock, Epidemiology and Infection, 125, (1) 221 - 224, 2000 Salmonella Abortusequi strains of equine origin harbor a 95kb plasmid responsible for virulence in mice, Masato Akiba; Toshiya Sameshima; Toru Anzai; Ryuichi Wada; Muneo Nakazawa, Veterinary Microbiology, 68, (3-4) 265 - 272, 1999 08 31 Molecular typing of Escherichia coli O157:H7 (H-) isolates from cattle in Japan, M. Akiba; T. Masuda; T. Sameshima; K. Katsuda; M. Nakazawa, Epidemiology and Infection, 122, (2) 337 - 341, 1999 04 Swine as a potential reservoir of shiga toxin-producing Escherichia coli O157:H7 in Japan [1], M. Nakazawa; M. Akiba; T. Sameshima, Emerging Infectious Diseases, Centers for Disease Control and Prevention (CDC), 5, (6) 833 - 834, 1999 Characterisation of Escherichia coli O157:H7 (H-) derived from cattle using random amplification of polymorphic DNA analysis, Ken Katsuda; Toshiya Sameshima; Masato Akiba; Muneo Nakazawa, Research in Veterinary Science, Elsevier, 65, (2) 177 - 178, 1998 Clonal turnover of enterohemorrhagic Escherichia coli O157: H7 in experimentally infected cattle, FEMS Microbiol. Lett. (2000), 184(1), 79-83 The shift of genetic subtypes of Escherichia coli O157: H7 isolates from cattle, Epidemiol. Infect. (1999), 122 (2), 343-346
Misc
サルモネラ認識を低下させる豚TLR5の一塩基多型とその豚サルモネラ症に及ぼす影響の検証, 宗田吉広; 新井暢夫; 新井暢夫; 矢ヶ部陽子; 新開浩樹; 芝原友幸; 江口正浩; 上西博英; 秋庭正人, 日本獣医学会学術集会講演要旨集, 161回, 2018 08 Comparative Analysis of Pathogenic Escherichia coli Isolated from Diseased Swine in Ibaraki Prefecture, Japan : Analysis of the Most Prevalent Serogroup O116, 藤井 勇紀; 田邊 ひとみ; 西野 弘人; 大谷 芳子; 都筑 智子; 大内 義尚; 秋庭 正人; 楠本 正博, 日本獣医師会雑誌 = Journal of the Japan Veterinary Medical Association, 70, (10) 643 - 649, 2017 10 Studies on porcine TLR5 SNP (C1205T) which related with impaired recognition of Salmonella. SNP discovery, functional analysis, genotyping method, frequency distribution, and experimental infection, Muneta Y; Arai N; Yakabe, Y; Eguchi M; Shibahara, T; Sakuma, A; Shinkai, H; Uenishi, H; Hirose K; Akiba M, Proceedings of the Japanese Pig Veterinary Society, (70) 25 - 31, 2017 08 サルモネラ薬剤耐性アイランド多コピー化の様式解明, 李謙一; 関塚剛史; 黒田誠; 楠本正博; 内田郁夫; 岩田剛敏; 岡本晋; 矢部希見子; 稲岡隆史; 秋庭正人, 日本獣医学会学術集会講演要旨集, 157th, 2014 08 11 HPCD子豚へのSalmonella enterica serovar Choleraesuis実験感染, 溝呂木 聖子; 矢ヶ部 陽子; 芝原 友幸; 秋庭 正人; 楠本 正博; 佐久間 晶子; 中條 満; 新開 浩樹; 上西 博英; 宗田 吉広, 日本豚病研究会報, (63) 6 - 11, 2014 02 , Toll-like receptor5(TLR5)は細菌のべん毛タンパク質(flagellin)を認識するパターン認識受容体で、豚ゲノムの解析においてサルモネラのflagellin及びSalmonella enterica serovar Choleraesuis(SC)の認識を低下させるTLR5一塩基多型(single nucleotide polymorphism: SNP) (C1205T)が報告されている。当該SNPがヘテロ型のランドレース豚の雌雄を交配して得られる妊娠母豚の子宮切除術によって作出されたhysterectomy produced colostrum deprived(HPCD)子豚へSCを無菌ミルクに混ぜて2回の経口実験感染を行った結果、HPCD子豚へのSC経口感染により、これまで再現が困難であった下痢や敗血症を特徴とする、豚サルモネラ症の病態モデルの再現ができた。しかし当該TLR5 SNPのSC感染に対する影響については、感染初期での排菌量に有意差は認められず、生存日数への影響についても2回の実験からは確かめられなかった。その理由としてHPCD子豚ではflagellin刺激に対するIL-6応答の顕著な低下が原因である可能性が示唆された。 Analysis of intestinal bacterial flora transplanted from bovine to chicken, 楠本 正博; 岩田 剛敏; 秋庭 正人, 動物衛生研究所研究報告, (121) 31 - 33, 2014 薬剤耐性アイランドの多コピー化に基づくサルモネラの耐性増強, 李謙一; 稲岡隆史; 楠本正博; 内田郁夫; 岩田剛敏; 岡本晋; 矢部季見子; 秋庭正人, 日本獣医学会学術集会講演要旨集, 156th, 2013 08 30 Salmonella Choleraesuisの離乳子豚に対する感染試験:TLR5の一塩基多型(C1205T)の影響, 宗田吉広; 宗田吉広; 溝呂木聖子; 溝呂木聖子; 矢ケ部陽子; 芝原友幸; 秋庭正人; 楠本正博; 佐久間晶子; 中條満; 新開浩樹; 上西博英, 日本獣医学会学術集会講演要旨集, 156回, 2013 08 カンピロバクター研究の最前線 細胞表面糖鎖の構造変化がカンピロバクター環境抵抗性に及ぼす影響, 岩田 剛敏; 知久 和寛; 天野 憲一; 楠本 正博; 秋庭 正人, 日本細菌学雑誌, 68, (1) 106 - 106, 2013 02 , https://search-tp.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2013&ichushi_jid=J01077&link_issn=&doc_id=20130227320078&doc_link_id=%2Fck0saiki%2F2013%2F006801%2F084%2F0106-0106%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fck0saiki%2F2013%2F006801%2F084%2F0106-0106%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif 細胞表面糖鎖の構造変化がカンピロバクター環境抵抗性に及ぼす影響, 岩田 剛敏; 知久 和寛; 天野 憲一; 楠本 正博; 秋庭 正人, 日本細菌学雑誌, 68, (1) 138 - 138, 2013 02 , https://search-tp.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2013&ichushi_jid=J01077&link_issn=&doc_id=20130227320173&doc_link_id=%2Fck0saiki%2F2013%2F006801%2F179%2F0138-0138%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fck0saiki%2F2013%2F006801%2F179%2F0138-0138%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif カンピロバクターの細胞表面糖脂質の糖鎖欠損により鶏腸管内定着性が低下する, 岩田剛敏; 知久和寛; 天野憲一; 楠本正博; 亀山眞由美; 小野裕嗣; 秋庭正人, 農研機構動物衛生研究所成果情報(Web), 2013, 2013 カンピロバクターの胆汁酸抵抗性及び鶏腸管内定着に重要なLOS糖鎖構造, 岩田 剛敏; 知久 和寛; 天野 憲一; 楠本 正博; 秋庭 正人, 日本獣医学会学術集会講演要旨集, 154回, 2012 08 ブタTLR遺伝子の多型がサルモネラ認識に及ぼす影響の解析, 新開浩樹; 鈴木倫太郎; 秋庭正人; 奥村直彦; 上西博英, 日本畜産学会大会講演要旨, 114回, 2011 08 近年北海道で分離される牛由来Salmonella Typhimuriumの遺伝学的特徴, 玉村雪乃; 内田郁夫; 田中聖; 秋庭正人; 窪田宜之; 秦英司; 菅野徹; 畠間真一; 石原凉子, 農研機構動物衛生研究所成果情報(Web), 2011, 2011 Salmonella Typhimurium DT104は百日咳毒素様のADP‐リボシル化酵素を産生する, 内田郁夫; 石原涼子; 田中聖; 秦英司; 牧野壮一; 菅野徹; 畠間真一; 木嶋眞人; 秋庭正人; 渡部淳; 窪田宜之, 動物衛生研究成果情報, (9) 39 - 40, 2010 10 29 Avian pathogenic Escherichia coli infection as an extraintestinal pathogenic E. coli infection, 秋庭 正人, 医学のあゆみ, 235, (4) 345 - 350, 2010 10 23 Bactericidal effect of superfine nanobubble and high density ozone water on Campylobacter jejuni, 岩田 剛敏; 秋庭 正人; 高木 昌美, Research report of animal feed, 34, 2009 06 ヒトと動物に共通する腸内細菌のストレス耐性, 中澤 宗生; 秋庭 正人; 佐藤 国雄, 畜産の研究, 62, (2) 252 - 254, 2008 02 , 人獣共通感染性腸内細菌である腸管出血性大腸菌（EHEC）O157を含む志賀毒素産生性大腸菌、サルモネラ、エンテロバクターなどの各種ストレスに対する耐性を調べた。EHEC O157：H7、志賀毒素産生性大腸菌、一般大腸菌はサルモネラやエンテロバクターに比べ高い酸耐性を示した。EHEC O157の酸耐性は一般大腸菌と比較しても有意に高かく、熱耐性でも、他の大腸菌やサルモネラより高い数値を示した。ヒトのEHEC O157：H7の感染菌量は100個未満であり、少量菌で感染が成立する要因の一つとして本菌のもつ強い酸耐性が考えられた。また、サルモネラの熱耐性では、感染宿主域が狭く種特異性が高い血清型Dublin、TyphisuisおよびPullormは熱ストレスに対してまったく抵抗できなかったが、宿主域が広く種特異性の低い血清型Enteritidis、Typhimurium、Agona、Infantis、SenftenbergおよびVirchowは0.001％〜30.0％の生残率を示した。サルモネラ食中毒の主要血清型であるこれら菌型のもつストレス耐性能は、環境や食品中での生存性に関与する要因の一つである考えられた。以上のように、腸内細菌のストレス耐性は菌種よる違いがあるが、強いストレス耐性能を有する菌型が存在することから、これらの特性を考慮した制御法が必要である。 Serotype of Salmonella enterica subsp. enterica sent to our laboratory between 1996-2006, 吉井 紀代; 秋庭 正人; 鮫島 俊哉, Bulletin of the National Institute of Animal Health, (114) 45 - 50, 2007 , 1996～2006年の11年間に動物衛生研究所腸内系細菌ユニット(旧ズーノーシス研究室)に公的機関から送付された動物由来サルモネラの血清型についてまとめた。牛由来19血清型158株，豚由来17血清型100株，鶏由来60血清型605株，その他の動物由来11血清型78株であった。家畜由来サルモネラの主要血清型は1980～1995年送付株と1996～2006年送付株で大部分は共通であった。一方，どの家畜由来株でも1980～1995年よりも1996～2006年の送付株で抗原構造表に記載のない血清型の割合が増加していた。 Salmonella Typhimurium DT104はADP‐ribosyltransferase毒素と相同性を示す遺伝子(artAB)を保持している, 内田郁夫; 西森敬; 田中聖; 牧野壮一; 菅野徹; 石原涼子; 畠間真一; 北野理恵; 木嶋眞人; 鮫島俊哉; 秋庭正人; 中沢宗生; 横溝祐一; 斉藤真理子, 動物衛生研究成果情報, (5) 31 - 32, 2006 08 31 牛由来サルモネラの薬剤耐性に関する最近の知見, 秋庭正人, 臨床獣医, 24, 2006 牛由来大腸菌O157:H7に関する分子疫学的研究 (特集 家畜衛生と疫学), 秋庭 正人, 畜産の研究, 54, (10) 1065 - 1072, 2000 10 パルスフィールドゲル電気泳動による腸管出血性大腸菌O157:H7の分子疫学的解析法, 秋庭 正人, 獣医畜産新報, 53, (1) 14 - 21, 2000 01 Fecal Shedding and Serum Antibody Responses of Cattle Experimentally Infected with Enterohaemorrhagic Escherichia coli O157 : H7, AKIBA Masato; SAMEJIMA Toshiya; MIYAZAWA Hiroshi; SHINAGAWA Kunihiro; NAKAZAWA Muneo, 感染症学雑誌 : 日本伝染病学会機関誌 : the journal of the Japanese Association for Infectious Diseases, 73, (10) 1082 - 1083, 1999 10 20 Carrier State of Escherichia coli O157 : H7 in Cattle, AKIBA Masato; SAMEJIMA Toshiya; NAKAZAWA Muneo, 感染症学雑誌 : 日本伝染病学会機関誌 : the journal of the Japanese Association for Infectious Diseases, 72, (2) 167 - 168, 1998 02 20 Serotype of Salmonella choleraesuis subsp. Choleraesuis isolated from domestic animals., 秋庭正人; 大宅辰夫; 三森真琴; 鮫島俊哉; 中沢宗生, 農林水産省家畜衛生試験場研究報告, (102/103) 43 - 48, 1996 10
Research Grants & Projects
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Nation-wide spatio-temporal phylogenomics of E. coli O157 and search for high risk O157 lineages, 20160401, 20190331, Hayashi Tetsuya; Ogura Yoshitoshi; IYODA Sunao; KUSUMOTO Masahiro, We sequenced 2,354 clinical O157 strains isolated in 2005, 2010, 2015 in Japan, which covered most strains isolated in each year in Japan, and 271 strains that were isolated from bovine between 1996 and 2017 in 19 prefectures in Japan. Using the core SNP information obtained from these draft sequences, we performed high-resolution phylogenetic analysis and identified several sublineages dominating in Japan and the turnovers of the dominant clones or sublineages. By developing a novel Stx2 subtyping method, we identified the difference in dominant subtype between sublineages and subtype change in several sublineages. We are now performing more detailed analyses, including analysis of sublineage specific genes and integrated analyses of clinical and bacterial genome information to identify high-risk O157 clones or sublineages and their dynamics. Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Amplification of a novel chromosomal resistance island of Salmonella Typhimurium confers increased resistance to antimicrobials, 20130401, 20160331, AKIBA Masato; KURODA Makoto, We found that antimicrobial resistance of Salmonella Typhimurium increased by gene amplification of a novel chromosomal resistance island (GI). Copy number of GI increased up to 85 and the elevation was accompanied by increased amount of transcription. The amplification occurred at the original position. In some cases, the transposition of part of GI occurred after insertion of IS26 or IS1, leading to the amplification of GI at different position. Elevation of transcription of SOS response related genes was observed in a comprehensive gene expression analysis. 日本学術振興会, 科学研究費助成事業, インドにおける医薬品及び薬剤耐性菌環境汚染調査, 20120401, 20160331, グルゲ キールティ・シリ; 山下 信義; 秋庭 正人, 2014年にチェンナイより採集した河川水・底質試料について医薬品及び有害化学物質の分析を行った。先進国では過去50年以上にわたって汚染が進行している有機過フッ素化合物や有機シロキサンについては、インドの汚染は始まったばかりであることが柱状底質試料を用いた歴史的復元により明らかとなった。医薬品ではイブプロフェンの濃度が最も高く、薬剤耐性菌への関与が疑われるキノロン剤のオフロキサシン濃度が次いで高濃度であった。
また、同じ底質試料の変異原性強度の空間的と鉛直分布を、コメットアッセイを用いて、多環芳香族炭化水素（PAHs）、およびそのハロゲン化物を指標として判定した。チェンナイ市・クーム川の底質は石油起源のPAHsによる高度な汚染が検証された。底質の変異原性の強さは、PAHsの汚染度に関連していた。また、４種類の下水汚泥の抽出物を雄性マウスに摂取させ、肝臓の遺伝子発現の変化をマイクロアレイ解析した。200以上の遺伝子について、それぞれの下水処理施設に特有な異なる誘導が見いだされ、排水の性質は排出元に大きく影響されることが示唆された。
さらに、これまでに分離した446株の大腸菌のうち、セフォタキシムやイミペネムといった人の治療に汎用されるβ-ラクタム剤に耐性を示す169株（38%）の薬剤耐性因子等を詳細に解析した。12の抗菌剤に対する薬剤感受性を調べたところ2剤を除いて50%以上の耐性菌分布率を示した。また、全ての菌は3剤以上に耐性を示し、最も分布率が高かったのは10剤耐性菌（25%）であった。β-ラクタマーゼ遺伝子型別のうち、CTX-M型の分布率が66%と最も高く、次いでTEM、OXA、CIT型の分布率がそれぞれ約40%であった。無作為に選んだ36の環境水サンプルに由来する49プラスミドの全塩基配列を解読し、β-ラクタマーゼ遺伝子を含む各プラスミドの系統と薬剤耐性遺伝子の分布を明らかにした。 日本学術振興会, 科学研究費助成事業, わが国の動物由来サルモネラにおけるセフェム系抗菌剤耐性に関する分子遺伝学的研究, 2010, 2011, 秋庭 正人; PETERFRANCIS SHAHADA; PETER FRANCIS Shahada, 抗菌剤が使用される家畜の生産現場は薬剤耐性菌選択の場の一つと考えられている。家畜由来薬剤耐性菌がヒトに感染した場合、その治療を難しくする可能性があるため、家畜由来食中毒菌の薬剤耐性化は公衆衛生上、看過できない。とりわけ、ヒトの治療に汎用される広域セファロスポリン(ESC)に対する耐性菌の出現には注意を払う必要がある。
2004年以降、北海道の牛においてESCに耐性を示す特定遺伝子型のSalmonella Typhimurium(ST-VII-6)が分離されている。本研究では、こめESC耐性が染色体上の多剤耐性ゲノミックアイランドの一つであるGI-VII-6に規定されていることを明らかにした。GI-VII-6の全長は125kbでESC耐性を規定するCMY-2 β-ラクタマーゼ遺伝子の他、10の薬剤耐性遺伝子を含んでおり、その塩基配列の特徴から薬剤耐性プラスミドが染色体上に転移したものと推察された。解析した22株のST-VII-6は全てGI-VII-6を保有しており、ユニークなSalmonella Typhimurium(ST)クローンが北海道の牛に拡散したものと推察された。
また、1農場で発生した牛サルモネラ症から薬剤耐性パターンの異なるST 4株が分離されたので諸性状を解析したところ、プラスミドプロファイルに違いは認められるものの、由来は同一の菌株で農場内において保有プラスミドが変化したものと推察された。薬剤耐性パターンは保有プラスミドによるため菌株の遺伝子型による近縁関係を反映するとは限らないことが示された。
さらに南九州地域のブロイラーからESCに耐性を示すサルモネラ血清型Infantis、Manhattan、および大腸菌を分離し、菌種内および菌種間の薬剤耐性プラスミド伝達の有無を調べたところ、両菌種内で薬剤耐性プラスミドの交換が起こっている可能性を指摘することができた。加えて、これらプラスミドが実験的に他菌種に伝達可能であることを示した。
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Last Update :2024/01/31
Researcher Profile and Settings
Name
| AKIBA Masato |
Affiliation (Master)
| School of Veterinary Medicine, Department of Veterinary Medicine |
Affiliation & Job
| Rakuno Gakuen University |
| Veterinary Bacteriology, Department of Pathobiology, School of Veterinary Medicine |
| Professor |
Degree
| Doctor of Veterinary Science, The University of Tokyo |
Association Memberships
| 動物用抗菌剤研究会 |
| 日本カンピロバクター研究会 |
| 腸管出血性大腸菌感染症研究会 |
| JAPANESE SOCIETY FOR BACTERIOLOGY |
| JAPANESE SOCIETY OF VETERINARY SCIENCE |
Research Activities
Research Areas
| Life sciences, Veterinary medicine |
| Life sciences, Hygiene and public health (laboratory) |
| Life sciences, Bacteriology |
Research Interests
| animal, |
| infectious disease, |
| bacteria, |
| molecular epidemiology, |
| pathogenesis, |
| antimicrobial resistance, |
Published Papers
| Characteristics of antimicrobial residues in manure composts from swine farms: Residual patterns, removal efficiencies, and relation to purchased quantities and composting methods in Japan, Mafumi Watanabe; Prasun Goswami; Katsumasa Kure; Itsuro Yamane; Sota Kobayashi; Masato Akiba; Keerthi S. Guruge, Journal of Hazardous Materials, 2023 08 |
| Nationwide analysis of antimicrobial resistance in pathogenic Escherichia coli strains isolated from diseased swine over 29 years in Japan, Masahiro Kusumoto; Yukino Tamamura-Andoh; Yuna Hikoda-Kogiku; Asami Magome; Erina Okuhama; Keisuke Sato; Yoshino Mizuno; Nobuo Arai; Ayako Watanabe-Yanai; Taketoshi Iwata; Yoshitoshi Ogura; Yasuhiro Gotoh; Keiji Nakamura; Tetsuya Hayashi; Masato Akiba, Frontiers in Microbiology, Frontiers Media SA, 14, 2023 03 17 |
| Occurrence and the potential ecological risk of veterinary antimicrobials in swine farm wastewaters in Japan: Seasonal changes, relation to purchased quantity and after termination of oxytetracycline usage, Mafumi Watanabe; Keerthi S Guruge; Ryuichi Uegaki; Katsumasa Kure; Itsuro Yamane; Sota Kobayashi; Masato Akiba, Environment International, 173, 107812 - 107812, 2023 03 |
| Tryptanthrin Reduces Campylobacter jejuni Colonization in the Chicken Gut by a Bactericidal Mechanism., Taketoshi Iwata; Ayako Watanabe-Yanai; Yukino Tamamura-Andoh; Nobuo Arai; Masato Akiba; Masahiro Kusumoto, Applied and environmental microbiology, 89, (2) , 2023 02 28 |
| Duplication of blaCTX-M-1 and a class 1 integron on the chromosome enhances antimicrobial resistance in Escherichia coli isolated from racehorses in Japan., Yukino Tamamura-Andoh; Hideaki Niwa; Yuta Kinoshita; Eri Uchida-Fujii; Nobuo Arai; Ayako Watanabe-Yanai; Taketoshi Iwata; Masato Akiba; Masahiro Kusumoto, Journal of global antimicrobial resistance, 27, 225 - 227, 2021 12 |
| The association between antimicrobials and the antimicrobial-resistant phenotypes and resistance genes of Escherichia coli isolated from hospital wastewaters and adjacent surface waters in Sri Lanka, Keerthi S. Guruge; Yukino A. Tamamura; Prasun Goswami; Rumi Tanoue; K.B.S.N. Jinadasa; Kei Nomiyama; Takeshi Ohura; Tatsuya Kunisue; Shinsuke Tanabe; Masato Akiba, Chemosphere, Elsevier BV, 279, 130591 - 130591, 2021 09 |
| Identification of a Recently Dominant Sublineage in Salmonella 4,[5],12:i:- Sequence Type 34 Isolated From Food Animals in Japan, Nobuo Arai; Tsuyoshi Sekizuka; Yukino Tamamura-Andoh; Lisa Barco; Atsushi Hinenoya; Shinji Yamasaki; Taketoshi Iwata; Ayako Watanabe-Yanai; Makoto Kuroda; Masato Akiba; Masahiro Kusumoto, Frontiers in Microbiology, Frontiers Media SA, 12, 2021 07 01 |
| A survey of antimicrobial resistance in Escherichia coli isolated from wild sika deer (Cervus nippon) in Japan, Yukino TAMAMURA-ANDOH; Nobuyuki TANAKA; Keisuke SATO; Yoshino MIZUNO; Nobuo ARAI; Ayako WATANABE-YANAI; Masato AKIBA; Masahiro KUSUMOTO, Journal of Veterinary Medical Science, Japanese Society of Veterinary Science, 83, (5) 754 - 758, 2021 |
| Influence of SOS-inducing agents on the expression of ArtAB toxin gene in Salmonella enterica and Salmonella bongori., Shou Miura; Yukino Tamamura; Mariko Takayasu; Miwa Sasaki; Natsuko Nishimura; Kanetaka Tokugawa; Izumi Suwa; Ryo Murata; Masato Akiba; Masahiro Kusumoto; Ikuo Uchida, Microbiology (Reading, England), 166, (8) 785 - 793, 2020 08 |
| Occurrence of antimicrobial-resistant Escherichia coli in sewage treatment plants of South India, Praveenkumarreddy Yerabham; Masato Akiba; Keerthi Siri Guruge; Keshava Balakrishna; Kalwaje Eshwara Vandana; Virendra Kumar, JOURNAL OF WATER SANITATION AND HYGIENE FOR DEVELOPMENT, IWA PUBLISHING, 10, (1) 48 - 55, 2020 03 |
| Molecular Epidemiology of <i>Campylobacter fetus</i> Isolates from Cattle in the Tokachi Area of Hokkaido, Japan, Hiroyasu TAKAHASHI; Taketoshi IWATA; Kiyoko NOBUMOTO; Erika OKAMOTO; Masato AKIBA; Satoshi TACHIBANA, Journal of the Japan Veterinary Medical Association, Japan Veterinary Medical Association, 72, (12) 750 - 756, 2019 12 20 |
| Identification of an Antigen Specific to a Protective Salmonella Monoclonal Antibody, Swarmistha Devi ARIBAM; Yumiko MASUKAGAMI; Kazumasa SHIRAIWA; Sayaka NISHIKAWA; Yohsuke OGAWA; Masato AKIBA; Yoshihiro SHIMOJI; Masahiro EGUCHI, JARQ, 53, (3) , 2019 07 |
| Salmonella Genomic Island 3 Is an Integrative and Conjugative Element and Contributes to Copper and Arsenic Tolerance of Salmonella enterica, Nobuo Arai; Tsuyoshi Sekizuka; Yukino Tamamura; Masahiro Kusumoto; Atsushi Hinenoya; Shinji Yamasaki; Taketoshi Iwata; Ayako Watanabe-Yanai; Makoto Kuroda; Masato Akiba, Antimicrobial Agents and Chemotherapy, American Society for Microbiology, 63, (9) , 2019 06 17 |
| Transcriptomic analysis of Campylobacter jejuni grown in a medium containing serine as the main energy source, Ayako Watanabe-YanaiTaketoshi IwataMasahiro KusumotoYukino TamamuraMasato Akiba, Archives of Microbiology, 2018 11 |
| In vivo effect of a TLR5 SNP (C1205T) on Salmonella enterica serovar Typhimurium infection in weaned, specific pathogen-free Landrace piglets, Yoshihiro Muneta; Nobuo Arai; Yoko Yakabe; Masahiro Eguchi; Tomoyuki Shibahara; Akiko Sakuma; Hiroki Shinkai; Hirohide Uenishi; Kensuke Hirose; Masato Akiba, Microbiology and Immunology, Wiley, 62, (6) 380 - 387, 2018 06 |
| Phylogenetic Characterization ofSalmonella entericaSerovar Typhimurium and Its Monophasic Variant Isolated from Food Animals in Japan Revealed Replacement of Major Epidemic Clones in the Last 4 Decades, Nobuo Arai; Tsuyoshi Sekizuka; Yukino Tamamura; Kiyoshi Tanaka; Lisa Barco; Hidemasa Izumiya; Masahiro Kusumoto; Atsushi Hinenoya; Shinji Yamasaki; Taketoshi Iwata; Ayako Watanabe; Makoto Kuroda; Ikuo Uchida; Masato Akiba, Journal of Clinical Microbiology, American Society for Microbiology, 56, (5) , 2018 02 28 |
| Seasonal movement of trace-element discharge in a typical south-Indian suburban community, Derrick Ian Joshua; Sumith Abeykoon; Izumi Watanabe; Lucyna Paszek; Keshava Balakrishna; Masato Akiba; Keerthi Siri Guruge, Water Science and Technology, IWA Publishing, 77, (4) 1035 - 1047, 2018 02 01 |
| Population structure of Escherichia coli O26 : H11 with recent and repeated stx2 acquisition in multiple lineages., Yoshitoshi Ogura; Yasuhiro Gotoh; Takehiko Itoh; Mitsuhiko P Sato; Kazuko Seto; Shyuji Yoshino; Junko Isobe; Yoshiki Etoh; Mariko Kurogi; Keiko Kimata; Eriko Maeda; Denis Piérard; Masahiro Kusumoto; Masato Akiba; Kiyoshi Tominaga; Yumi Kirino; Yuki Kato; Katsuhiko Shirahige; Tadasuke Ooka; Nozomi Ishijima; Ken-Ichi Lee; Sunao Iyoda; Jacques Georges Mainil; Tetsuya Hayashi, Microbial genomics, 3, (11) , 2017 11 |
| Nationwide investigation of Shiga toxin-producing Escherichia coli among cattle in Japan revealed the risk factors and potentially virulent subgroups, K. Lee; M. Kusumoto; T. Iwata; S. Iyoda; M. Akiba, EPIDEMIOLOGY AND INFECTION, CAMBRIDGE UNIV PRESS, 145, (8) 1557 - 1566, 2017 06 |
| Determination of O:4 antigen-antibody affinity level in O: 5 antigen positive and negative variants of Salmonella enterica serovar Typhimurium, Yuka Nakai; Akihisa Ito; Yohsuke Ogawa; Swarmistha Devi Aribam; Marta Elsheimer-Matulova; Kazumasa Shiraiwa; Stevens M. B. Kisaka; Hirokazu Hikono; Sayaka Nishikawa; Masato Akiba; Kazuyoshi Kawahara; Yoshihiro Shimoji; Masahiro Eguchi, FEMS MICROBIOLOGY LETTERS, OXFORD UNIV PRESS, 364, (7) , 2017 04 |
| Trace element distribution and risk assessment in South Indian surface waterways, K. S. Guruge; P. Goswami; I. Watanabe; S. Abeykoon; V. P. Prabhasankar; K. R. Binu; D. I. Joshua; K. Balakrishna; M. Akiba; N. Munuswamy, INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY, SPRINGER, 14, (1) 1 - 18, 2017 01 |
| Transcription factor-mediated induction of gene expression and their association with the prevalence of antimicrobial-resistant Escherichia coli in wastewater, K. S. Guruge; N. Yamanaka; M. Yoshioka; M. Akiba; T. Yamamoto; T. Tsutsui; K. Balakrishna, TOXICOLOGY LETTERS, ELSEVIER IRELAND LTD, 259, S125 - S126, 2016 10 |
| Peptidoglycan Acetylation of Campylobacter jejuni Is Essential for Maintaining Cell Wall Integrity and Colonization in Chicken Intestines, Taketoshi Iwata; Ayako Watanabe; Masahiro Kusumoto; Masato Akiba, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMER SOC MICROBIOLOGY, 82, (20) 6284 - 6290, 2016 10 |
| Spatio-temporal distribution, source, and genotoxic potential of polycyclic aromatic hydrocarbons in estuarine and riverine sediments from southern India, Prasun Goswami; Takeshi Ohura; Keerthi S. Guruge; Miyako Yoshioka; Noriko Yamanaka; Masato Akiba; Natesan Munuswamy, ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, ACADEMIC PRESS INC ELSEVIER SCIENCE, 130, 113 - 123, 2016 08 |
| Colistin-Resistant mcr-1-Positive Pathogenic Escherichia coil in Swine, Japan, 2007-2014, Masahiro Kusumoto; Yoshitoshi Ogura; Yasuhiro Gotoh; Taketoshi Iwata; Tetsuya Hayashi; Masato Akiba, EMERGING INFECTIOUS DISEASES, CENTERS DISEASE CONTROL, 22, (7) 1315 - 1317, 2016 07 |
| Removal rates of antibiotics in four sewage treatment plants in South India, Valiparambil Prabhakaranunni Prabhasankar; Derrick Ian Joshua; Keshava Balakrishna; Iyanee Faroza Siddiqui; Sachi Taniyasu; Nobuyoshi Yamashita; Kurunthachalam Kannan; Masato Akiba; Yerabham Praveenkumarreddy; Keerthi S. Guruge, ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, SPRINGER HEIDELBERG, 23, (9) 8679 - 8685, 2016 05 |
| Distribution and Relationships of Antimicrobial Resistance Determinants among Extended-Spectrum-Cephalosporin-Resistant or Carbapenem-Resistant Escherichia coli Isolates from Rivers and Sewage Treatment Plants in India, Masato Akiba; Tsuyoshi Sekizuka; Akifumi Yamashita; Makoto Kuroda; Yuki Fujii; Misato Murata; Ken-ichi Lee; Derrick Ian Joshua; Keshava Balakrishna; Indira Bairy; Kaushik Subramanian; Padma Krishnan; Natesan Munuswamy; Ravindra K. Sinha; Taketoshi Iwata; Masahiro Kusumoto; Keerthi S. Guruge, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, AMER SOC MICROBIOLOGY, 60, (5) 2972 - 2980, 2016 05 |
| Emergence of a Multidrug-Resistant Shiga Toxin-Producing Enterotoxigenic Escherichia coli Lineage in Diseased Swine in Japan, Masahiro Kusumoto; Yuna Hikoda; Yuki Fujii; Misato Murata; Hirotsugu Miyoshi; Yoshitoshi Ogura; Yasuhiro Gotoh; Taketoshi Iwata; Tetsuya Hayashi; Masato Akiba, JOURNAL OF CLINICAL MICROBIOLOGY, AMER SOC MICROBIOLOGY, 54, (4) 1074 - 1081, 2016 04 |
| Investigation of a plasmid genome database for colistin-resistance gene mcr-1, Satowa Suzuki; Mamoru Ohnishi; Michiko Kawanishi; Masato Akiba; Makoto Kuroda, LANCET INFECTIOUS DISEASES, ELSEVIER SCI LTD, 16, (3) 284 - 285, 2016 03 |
| Source-Related Effects of Wastewater on Transcription Factor (AhR, CAR and PXR)-Mediated Induction of Gene Expression in Cultured Rat Hepatocytes and Their Association with the Prevalence of Antimicrobial-Resistant Escherichia coli, Keerthi S. Guruge; Noriko Yamanaka; Miyuki Sonobe; Wataru Fujizono; Miyako Yoshioka; Masato Akiba; Takehisa Yamamoto; Derrick I. Joshua; Keshava Balakrishna; Nobuyoshi Yamashita; Kurunthachalam Kannan; Toshiyuki Tsutsui, PLOS ONE, PUBLIC LIBRARY SCIENCE, 10, (9) , 2015 09 |
| Lipooligosaccharide Core Truncations Affect the Ability of Campylobacter jejuni to Attach to Glass and Form Biofilms under Aerobic Conditions, Vu Tuan Nguyen; Taketoshi Iwata; Masahiro Kusumoto; Masato Akiba, JARQ-JAPAN AGRICULTURAL RESEARCH QUARTERLY, JAPAN INT RESEARCH CENTER AGRICULTURAL SCIENCES, 49, (3) 269 - 275, 2015 07 |
| Molecular typing of Salmonella enterica serovar 4,[5],12:i:- isolates from humans, animals and river water in Japan by multilocus variable-number tandem repeat analysis and pulsed-field gel electrophoresis, Noriko Ido; Kaori Iwabuchi; Yusuke Sato'o; Yasuo Sato; Masaru Sugawara; Gakuji Yaegashi; Masaru Konno; Masato Akiba; Kiyoshi Tanaka; Katsuhiko Omoe; Ikuo Uchida, JOURNAL OF VETERINARY MEDICAL SCIENCE, JAPAN SOC VET SCI, 77, (5) 609 - 613, 2015 05 |
| Impact of wastewater from different sources on the prevalence of antimicrobial-resistant Escherichia coli in sewage treatment plants in South India, Masato Akiba; Hironobu Senba; Haruna Otagiri; Valipparambil P. Prabhasankar; Sachi Taniyasu; Nobuyoshi Yamashita; Ken-ichi Lee; Takehisa Yamamoto; Toshiyuki Tsutsui; Derrick Ian Joshua; Keshava Balakrishna; Indira Bairy; Taketoshi Iwata; Masahiro Kusumoto; Kurunthachalam Kannan; Keerthi S. Guruge, ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, ACADEMIC PRESS INC ELSEVIER SCIENCE, 115, 203 - 208, 2015 05 |
| Extensive amplification of GI-VII-6, a multidrug resistance genomic island of Salmonella enterica serovar Typhimurium, increases resistance to extended-spectrum cephalosporins, Ken-ichi Lee; Masahiro Kusumoto; Tsuyoshi Sekizuka; Makoto Kuroda; Ikuo Uchida; Taketoshi Iwata; Susumu Okamoto; Kimiko Yabe; Takashi Inaoka; Masato Akiba, FRONTIERS IN MICROBIOLOGY, FRONTIERS RESEARCH FOUNDATION, 6, 2015 02 |
| Monoclonal antibody-based competitive enzyme-linked immunosorbent assay to detect antibodies to O:4 Salmonella in the sera of livestock and poultry, Swarmistha Devi Aribam; Yohsuke Ogawa; Hidenori Matsui; Jiro Hirota; Masashi Okamura; Masato Akiba; Yoshihiro Shimoji; Masahiro Eguchi, JOURNAL OF MICROBIOLOGICAL METHODS, ELSEVIER SCIENCE BV, 108, 1 - 3, 2015 01 |
| Characteristics of Salmonella enterica Serovar 4,[5], 12: i:- as a Monophasic Variant of Serovar Typhimurium, Noriko Ido; Ken-ichi Lee; Kaori Iwabuchi; Hidemasa Izumiya; Ikuo Uchida; Masahiro Kusumoto; Taketoshi Iwata; Makoto Ohnishi; Masato Akiba, PLOS ONE, PUBLIC LIBRARY SCIENCE, 9, (8) , 2014 08 |
| Molecular typing of Salmonella enterica serovar Enteritidis isolates from food-producing animals in Japan by multilocus variable-number tandem repeat analysis: evidence of clonal dissemination and replacement, Ayumi Kobayashi; Sayaka Takahashi; Masaaki Ono; Kiyoshi Tanaka; Masato Kishima; Masato Akiba; Ikuo Uchida, ACTA VETERINARIA SCANDINAVICA, BIOMED CENTRAL LTD, 56, 2014 05 |
| HPCD子豚へのSalmonella enterica serovar Choleraesuis実験感染 病態の再現とTLR5一塩基多型(C1205T)の影響検証の試み, 溝呂木 聖子; 矢ヶ部 陽子; 芝原 友幸; 秋庭 正人; 楠本 正博; 佐久間 晶子; 中條 満; 新開 浩樹; 上西 博英; 宗田 吉広, 日本豚病研究会報, 日本豚病研究会, (63) 6 - 11, 2014 02 |
| Lineage-Specific Distribution of Insertion Sequence Excision Enhancer in Enterotoxigenic Escherichia coli Isolated from Swine, Masahiro Kusumoto; Dai Fukamizu; Yoshitoshi Ogura; Eiji Yoshida; Fumiko Yamamoto; Taketoshi Iwata; Tadasuke Ooka; Masato Akiba; Tetsuya Hayashi, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMER SOC MICROBIOLOGY, 80, (4) 1394 - 1402, 2014 02 |
| Whole-genome sequence of CMY-2 β-lactamase-producing Salmonella enterica serovar Typhimurium strain L-3553, Tsuyoshi Sekizuka; Ken-Ichi Lee; Makoto Kuroda; Masahiro Kusumoto; Taketoshi Iwata; Ikuo Uchida; Kiyoshi Tanaka; Yukino Tamamura; Masato Akiba, Genome Announcements, American Society for Microbiology, 2, (4) , 2014 |
| Complete Nucleotide Sequences of Virulence-Resistance Plasmids Carried by Emerging Multidrug-Resistant Salmonella enterica Serovar Typhimurium Isolated from Cattle in Hokkaido, Japan, Yukino Tamamura; Kiyoshi Tanaka; Masato Akiba; Toru Kanno; Shinichi Hatama; Ryoko Ishihara; Ikuo Uchida, PLoS ONE, 8, (10) , 2013 10 14 |
| Distribution of extended-spectrum cephalosporin resistance determinants in Salmonella enterica and Escherichia coli isolated from broilers in southern Japan, F. Shahada; T. Chuma; G. Kosugi; M. Kusumoto; T. Iwata; M. Akiba, Poultry Science, 92, (6) 1641 - 1649, 2013 06 |
| Genomic Analysis of Salmonella enterica Serovar Typhimurium Definitive Phage Type 104, Hidemasa Izumiya; Jun Terajima; Shouji Yamamoto; Makoto Ohnishi; Haruo Watanabe; Akemi Kai; Takayuki Kurazono; Masumi Taguchi; Tetsuo Asai; Masato Akiba; Yuko Matsumoto; Yutaka Tamura, EMERGING INFECTIOUS DISEASES, CENTERS DISEASE CONTROL, 19, (5) 823 - 825, 2013 05 |
| Chronological change of resistance to beta-lactams in Salmonella enterica serovar Infantis isolated from broilers in Japan, Takehisa Chuma; Daisuke Miyasako; Hesham Dahshan; Tomoko Takayama; Yuko Nakamoto; Francis Shahada; Masato Akiba; Karoku Okamoto, FRONTIERS IN MICROBIOLOGY, FRONTIERS RESEARCH FOUNDATION, 4, 2013 05 |
| Effects of Lipooligosaccharide Inner Core Truncation on Bile Resistance and Chick Colonization by Campylobacter jejuni, Taketoshi Iwata; Kazuhiro Chiku; Ken-ichi Amano; Masahiro Kusumoto; Mayumi Ohnishi-Kameyama; Hiroshi Ono; Masato Akiba, PLoS ONE, 8, (2) , 2013 02 20 |
| Colonization of chicken flocks by Campylobacter jejuni in multiple farms in Japan, S. Yano; T. Kira; Y. Morishita; K. Ishihara; T. Asai; T. Iwata; M. Akiba; T. Murase, Poultry Science, 92, (2) 375 - 381, 2013 02 |
| Distinguishable epidemics of multidrug-resistant Salmonella typhimurium DT104 in different hosts, A. E. Mather; S. W.J. Reid; D. J. Maskell; J. Parkhill; M. C. Fookes; S. R. Harris; D. J. Brown; J. E. Coia; M. R. Mulvey; M. W. Gilmour; L. Petrovska; E. De Pinna; M. Kuroda; M. Akiba; H. Izumiya; T. R. Connor; M. A. Suchard; P. Lemey; D. J. Mellor; D. T. Haydon; N. R. Thomson, Science, American Association for the Advancement of Science, 341, (6153) 1514 - 1517, 2013 |
| Fluoroquinolone resistance mechanisms in an Escherichia coli isolate, HUE1, without quinolone resistance-determining region mutations, Toyotaka Sato; Shin-Ichi Yokota; Ikuo Uchida; Torahiko Okubo; Masaru Usui; Masahiro Kusumoto; Masato Akiba; Nobuhiro Fujii; Yutaka Tamura, Frontiers in Microbiology, Frontiers Research Foundation, 4, (MAY) , 2013 |
| Molecular typing of Salmonella enterica serotype Typhimurium and serotype 4,5,12:i:- isolates from cattle by multiple-locus variable-number tandem-repeats analysis, Atsushi Kurosawa; Takeshi Imamura; Kiyoshi Tanaka; Yukino Tamamura; Ikuo Uchida; Ayumi Kobayashi; Eiji Hata; Toru Kanno; Masato Akiba; Shouichirou Yukawa; Yutaka Tamura, VETERINARY MICROBIOLOGY, ELSEVIER SCIENCE BV, 160, (1-2) 264 - 268, 2012 11 |
| Change in Antimicrobial Resistance Pattern in Salmonella enterica serovar Typhimurium Isolates Detected in a Beef Cattle Farm, Masaru Sugawara; Francis Shahada; Hidemasa Izumiya; Haruo Watanabe; Ikuo Uchida; Yukino Tamamura; Masahiro Kusumoto; Taketoshi Iwata; Masato Akiba, JOURNAL OF VETERINARY MEDICAL SCIENCE, JAPAN SOC VET SCI, 74, (1) 93 - 97, 2012 01 |
| Characterization of Salmonella enterica Serovar Typhimurium Isolates Harboring a Chromosomally Encoded CMY-2 beta-Lactamase Gene Located on a Multidrug Resistance Genomic Island, Francis Shahada; Tsuyoshi Sekizuka; Makoto Kuroda; Masahiro Kusumoto; Daiki Ohishi; Atsuko Matsumoto; Hizuru Okazaki; Kiyoshi Tanaka; Ikuo Uchida; Hidemasa Izumiya; Haruo Watanabe; Yukino Tamamura; Taketoshi Iwata; Masato Akiba, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, AMER SOC MICROBIOLOGY, 55, (9) 4114 - 4121, 2011 09 |
| Porcine Toll-like receptors: Recognition of Salmonella enterica serovar Choleraesuis and influence of polymorphisms, Hiroki Shinkai; Rintaro Suzuki; Masato Akiba; Naohiko Okumura; Hirohide Uenishi, MOLECULAR IMMUNOLOGY, PERGAMON-ELSEVIER SCIENCE LTD, 48, (9-10) 1114 - 1120, 2011 05 |
| Rapid identification of Salmonella enterica serovars, Typhimurium, Choleraesuis, Infantis, Hadar, Enteritidis, Dublin and Gallinarum, by multiplex PCR, Masato Akiba; Masahiro Kusumoto; Taketoshi Iwata, JOURNAL OF MICROBIOLOGICAL METHODS, ELSEVIER SCIENCE BV, 85, (1) 9 - 15, 2011 04 |
| Molecular and Phenotypic Characteristics of CMY-2 beta-Lactamase-Producing Salmonella enterica Serovar Typhimurium Isolated from Cattle in Japan, Masaru Sugawara; Junko Komori; Marimo Kawakami; Hidemasa Izumiya; Haruo Watanabe; Masato Akiba, JOURNAL OF VETERINARY MEDICAL SCIENCE, JAPAN SOC VET SCI, 73, (3) 345 - 349, 2011 03 |
| Molecular Epidemiology of Salmonella enterica Serovar Typhimurium Isolates from Cattle in Hokkaido, Japan: Evidence of Clonal Replacement and Characterization of the Disseminated Clone, Yukino Tamamura; Ikuo Uchida; Kiyoshi Tanaka; Hizuru Okazaki; Satoru Tezuka; Hideki Hanyu; Natsumi Kataoka; Sou-ichi Makino; Masato Kishima; Takayuki Kubota; Toru Kanno; Shinichi Hatama; Ryoko Ishihara; Eiji Hata; Hironari Yamada; Yuuji Nakaoka; Masato Akiba, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMER SOC MICROBIOLOGY, 77, (5) 1739 - 1750, 2011 03 |
| Molecular and Phenotypic Characteristics of Salmonella enterica Serovar 4,5,12:i:- Isolated from Cattle and Humans in Iwate Prefecture, Japan, Noriko Ido; Takashi Kudo; Koji Sasaki; Masato Motokawa; Kaori Iwabuchi; Hiroki Matsudate; Yukio M. Seimiya; Masato Akiba, JOURNAL OF VETERINARY MEDICAL SCIENCE, JAPAN SOC VET SCI, 73, (2) 241 - 244, 2011 02 |
| Insertion sequence-excision enhancer removes transposable elements from bacterial genomes and induces various genomic deletions, Masahiro Kusumoto; Tadasuke Ooka; Yoshiaki Nishiya; Yoshitoshi Ogura; Takashi Saito; Yasuhiko Sekine; Taketoshi Iwata; Masato Akiba; Tetsuya Hayashi, NATURE COMMUNICATIONS, NATURE PUBLISHING GROUP, 2, 2011 01 |
| Identification of a Key Amino Acid of LuxS Involved in AI-2 Production in Campylobacter jejuni, Paul Plummer; Jinge Zhu; Masato Akiba; Dehua Pei; Qijing Zhang, PLOS ONE, PUBLIC LIBRARY SCIENCE, 6, (1) , 2011 01 |
| Characterization of Antibiotic Resistance and the Emergence of AmpC-Producing Salmonella Infantis from Pigs, Hesham Dahshan; Takehisa Chuma; Francis Shahada; Masato Akiba; Hideki Fujimoto; Keishirou Akasaka; Yuji Kamimura; Karoku Okamoto, JOURNAL OF VETERINARY MEDICAL SCIENCE, JAPAN SOC VET SCI, 72, (11) 1437 - 1442, 2010 11 |
| Detection and Characterization of Extended-Spectrum beta-Lactamase (TEM-52)-Producing Salmonella Serotype Infantis from Broilers in Japan, Francis Shahada; Takehisa Chuma; Hesham Dahshan; Masato Akiba; Masuo Sueyoshi; Karoku Okamoto, FOODBORNE PATHOGENS AND DISEASE, MARY ANN LIEBERT INC, 7, (5) 515 - 521, 2010 05 |
| Pulsed-Field Gel Electrophoresis Profile Changes Resulting from Spontaneous Chromosomal Deletions in Enterohemorrhagic Escherichia coli O157:H7 during Passage in Cattle, Noriyo Yoshii; Yoshitoshi Ogura; Tetsuya Hayashi; Takashi Ajiro; Toshiya Sameshima; Muneo Nakazawa; Masahiro Kusumoto; Taketoshi Iwata; Masato Akiba, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMER SOC MICROBIOLOGY, 75, (17) 5719 - 5726, 2009 09 |
| Salmonella enterica serotype Typhimurium DT104 ArtA-dependent modification of pertussis toxin-sensitive G proteins in the presence of [32P]NAD, Ikuo Uchida; Ryoko Ishihara; Kiyoshi Tanaka; Eiji Hata; Sou-Ichi Makino; Toru Kanno; Shinichi Hatama; Masato Kishima; Masato Akiba; Atsushi Watanabe; Takayuki Kubota, Microbiology, 155, (11) 3710 - 3718, 2009 |
| Phage type and antimicrobial susceptibility of Salmonella enterica serovar Enteritidis from food-producing animals in Japan between 1976 and 2004., Tetsuo Asai; Kazuki Harada; Akemi Kojima; Toshiya Sameshima; Toshio Takahashi; Masato Akiba; Muneo Nakazawa; Hidemasa Izumiya; Jun Terajima; Haruo Watanbe, The new microbiologica, 31, (4) 555 - 9, 2008 10 |
| Antimicrobial resistance of Salmonella enterica serovar Typhimurium isolated from cattle in Japan, Masato Akiba; Toshiya Sameshima; Ikuo Uchida; Muneo Nakazawa, Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment, 25, (9) 1076 - 1079, 2008 09 |
| Antimicrobial resistance of Salmonella enterica serovar Typhimurium isolated from cattle in Japan, Masato Akiba; Toshiya Sameshima; Ikuo Uchida; Muneo Nakazawa, FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, TAYLOR & FRANCIS LTD, 25, (9) 1076 - 1079, 2008 |
| Changes in antimicrobial susceptibility in a population of Salmonella enterica serovar Dublin isolated from cattle in Japan from 1976 to 2005, Masato Akiba; Yuji Nakaoka; Muneyoshi Kida; Yukiko Ishioka; Toshiya Sameshima; Noriyo Yoshii; Muneo Nakazawa; Ikuo Uchida; Nobuyuki Terakado, JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, OXFORD UNIV PRESS, 60, (6) 1235 - 1242, 2007 12 |
| Detection and characterization of variant Salmonella genomic island is from Salmonella Derby isolates, Masato Akiba; Kotaro Nakamura; David Shinoda; Noriyo Yoshii; Hiroya Ito; Ikuo Uchida; Muneo Nakazawa, JAPANESE JOURNAL OF INFECTIOUS DISEASES, NATL INST INFECTIOUS DISEASES, 59, (5) 341 - 345, 2006 10 |
| Comparison of PCR-RFLP and PFGE for determining the clonality of enterohemorrhagic Escherichia coli strains, Kensuke Shima; Noriyo Yoshii; Masato Akiba; Kazuhiko Nishimura; Muneo Nakazawa; Shinji Yamasaki, FEMS Microbiology Letters, 257, (1) 124 - 131, 2006 04 |
| Interaction of CmeABC and CmeDEF in conferring antimicrobial resistance and maintaining cell viability in Campylobacter jejuni, M Akiba; J Lin; YW Barton; QJ Zhang, JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, OXFORD UNIV PRESS, 57, (1) 52 - 60, 2006 01 |
| The artAB genes encode a putative ADP-ribosyltransferase toxin homologue associated with Salmonella enterica serovar Typhimurium DT104, Mariko Saitoh; Kiyoshi Tanaka; Kei Nishimori; Sou-Ichi Makino; Toru Kanno; Ryoko Ishihara; Shinichi Hatama; Rie Kitano; Masato Kishima; Toshiya Sameshima; Masato Akiba; Muneo Nakazawa; Yuichi Yokomizo; Ikuo Uchida, Microbiology, 151, (9) 3089 - 3096, 2005 09 |
| Contamination of Salmonella in retail meats and shrimps in the Mekong Delta, Vietnam, Thi Phan Tran; Ly Thi Lien Khai; Natsue Ogasawara; Thu Tam Nguyen; Alexandre Tomomitsu Okatani; Masato Akiba; Hideki Hayashidani, Journal of Food Protection, 68, (5) 1077 - 1080, 2005 05 |
| CmeR functions as a transcriptional repressor for the multidrug efflux pump CmeABC in Campylobacter jejuni, J Lin; M Akiba; O Sahin; QJ Zhang, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, AMER SOC MICROBIOLOGY, 49, (3) 1067 - 1075, 2005 03 |
| The importance of a 95-kb virulence plasmid in the pathogenicity of Salmonella abortusequi in horses, Toru Anzai; Yasushi Kuwamoto; Seiji Hobo; Hidekazu Niwa; Yoshinari Katayama; Hirotaka Ode; Nariaki Abe; Akira Doi; Masato Akiba; Toshiya Sameshima, Journal of Equine Science, 16, (4) 111 - 116, 2005 |
| Prevalence of Salmonella spp. in pigs, chickens and ducks in the Mekong Delta, Vietnam, Thi Phan Tran; Thi Lien Khai Ly; Thu Tan Nguyen; Masato Akiba; Natsue Ogasawara; David Shinoda; Alexandre Tomomitsu Okatani; Hideki Hayashidani, Journal of Veterinary Medical Science, 66, (8) 1011 - 1014, 2004 08 |
| Molecular Characterization of a Prophage of Salmonella enterica Serotype Typhimurium DT104, Kiyoshi Tanaka; Kei Nishimori; Sou-Ichi Makino; Tomoko Nishimori; Toru Kanno; Ryoko Ishihara; Toshiya Sameshima; Masato Akiba; Muneo Nakazawa; Yuichi Yokomizo; Ikuo Uchida, Journal of Clinical Microbiology, 42, (4) 1807 - 1812, 2004 04 |
| Analysis of the complete nucleotide sequence of an Actinobacillus pleuropneumoniae streptomycin-sulfonamide resistance plasmid, pMS260, Hiroya Ito; Hiroshi Ishii; Masato Akiba, Plasmid, Academic Press Inc., 51, (1) 41 - 47, 2004 |
| Virulence and other biological properties of Salmonella Typhimurium isolated from foals in Japan from 1981 until 1996, Toru Anzai; Yasushi Kuwamoto; Ryuichi Wada; Masato Akiba; Tatsuo Ohya; Toshiya Sameshima, Journal of Equine Science, 14, (3) 87 - 92, 2003 09 |
| Comparison of Salmonella enterica serovar Abortusequi isolates of equine origin by pulsed-field gel electrophoresis and fluorescent amplified-fragment length polymorphism fingerprinting, M Akiba; Uchida, I; K Nishimori; K Tanaka; T Anzai; Y Kuwamoto; R Wada; T Ohya; H Ito, VETERINARY MICROBIOLOGY, ELSEVIER SCIENCE BV, 92, (4) 379 - 388, 2003 04 |
| Isolation of Salmonella Strains from the Aquatic Environment and Comparison with those of Animal Origin in Tan Phu Thanh Village, Mekong Delta, Vietnam, Tran Thi Phan; Ly Thi Lien Khai; Chau Ba Loc; Hideki Hayashidani; Toshiya Sameshima; Takeshi Watanabe; Toshiaki Taniguchi; Hideki Kobayashi; Hiroya Ito; Masato Akiba, Japan Agricultural Research Quarterly, Japan International Research Center for Agricultural Sciences, 37, (4) 237 - 241, 2003 |
| Use of a trial probiotic product in calves experimentally infected with Escherichia coli O157, T Ohya; M Akiba; H Ito, JARQ-JAPAN AGRICULTURAL RESEARCH QUARTERLY, TROPICAL AGRICULTURE RES CENTR, 35, (3) 189 - 194, 2001 07 |
| Molecular typing and epidemiological study of Salmonella enterica serotype typhimurium isolates from cattle by fluorescent amplified-fragment length polymorphism fingerprinting and pulsed-field gel electrophoresis, Y Tamada; Y Nakaoka; K Nishimori; A Doi; T Kumaki; N Uemura; K Tanaka; SI Makino; T Sameshima; M Akiba; M Nakazawa; Uchida, I, JOURNAL OF CLINICAL MICROBIOLOGY, AMER SOC MICROBIOLOGY, 39, (3) 1057 - 1066, 2001 03 |
| Salmonella typhimurium DT104 from livestock in Japan, Toshiya Sameshima; Masato Akiba; Hidemasa Izumiya; Jun Terajima; Kazumiti Tamura; Haruo Watanabe; Muneo Nakazawa, Japanese Journal of Infectious Diseases, 53, (1) 15 - 16, 2000 02 |
| A comparison of Escherichia coli O157 isolates from cattle in Japan and the USA by molecular biological methods, M. Akiba; D. H. Rice; M. A. Davis; T. Masuda; T. Sameshima; M. Nakazawa; D. D. Hancock, Epidemiology and Infection, 125, (1) 221 - 224, 2000 |
| Salmonella Abortusequi strains of equine origin harbor a 95kb plasmid responsible for virulence in mice, Masato Akiba; Toshiya Sameshima; Toru Anzai; Ryuichi Wada; Muneo Nakazawa, Veterinary Microbiology, 68, (3-4) 265 - 272, 1999 08 31 |
| Molecular typing of Escherichia coli O157:H7 (H-) isolates from cattle in Japan, M. Akiba; T. Masuda; T. Sameshima; K. Katsuda; M. Nakazawa, Epidemiology and Infection, 122, (2) 337 - 341, 1999 04 |
| Swine as a potential reservoir of shiga toxin-producing Escherichia coli O157:H7 in Japan [1], M. Nakazawa; M. Akiba; T. Sameshima, Emerging Infectious Diseases, Centers for Disease Control and Prevention (CDC), 5, (6) 833 - 834, 1999 |
| Characterisation of Escherichia coli O157:H7 (H-) derived from cattle using random amplification of polymorphic DNA analysis, Ken Katsuda; Toshiya Sameshima; Masato Akiba; Muneo Nakazawa, Research in Veterinary Science, Elsevier, 65, (2) 177 - 178, 1998 |
| Clonal turnover of enterohemorrhagic Escherichia coli O157: H7 in experimentally infected cattle, FEMS Microbiol. Lett. (2000), 184(1), 79-83 |
| The shift of genetic subtypes of Escherichia coli O157: H7 isolates from cattle, Epidemiol. Infect. (1999), 122 (2), 343-346 |
Misc
| サルモネラ認識を低下させる豚TLR5の一塩基多型とその豚サルモネラ症に及ぼす影響の検証, 宗田吉広; 新井暢夫; 新井暢夫; 矢ヶ部陽子; 新開浩樹; 芝原友幸; 江口正浩; 上西博英; 秋庭正人, 日本獣医学会学術集会講演要旨集, 161回, 2018 08 |
| Comparative Analysis of Pathogenic Escherichia coli Isolated from Diseased Swine in Ibaraki Prefecture, Japan : Analysis of the Most Prevalent Serogroup O116, 藤井 勇紀; 田邊 ひとみ; 西野 弘人; 大谷 芳子; 都筑 智子; 大内 義尚; 秋庭 正人; 楠本 正博, 日本獣医師会雑誌 = Journal of the Japan Veterinary Medical Association, 70, (10) 643 - 649, 2017 10 |
| Studies on porcine TLR5 SNP (C1205T) which related with impaired recognition of Salmonella. SNP discovery, functional analysis, genotyping method, frequency distribution, and experimental infection, Muneta Y; Arai N; Yakabe, Y; Eguchi M; Shibahara, T; Sakuma, A; Shinkai, H; Uenishi, H; Hirose K; Akiba M, Proceedings of the Japanese Pig Veterinary Society, (70) 25 - 31, 2017 08 |
| サルモネラ薬剤耐性アイランド多コピー化の様式解明, 李謙一; 関塚剛史; 黒田誠; 楠本正博; 内田郁夫; 岩田剛敏; 岡本晋; 矢部希見子; 稲岡隆史; 秋庭正人, 日本獣医学会学術集会講演要旨集, 157th, 2014 08 11 |
| HPCD子豚へのSalmonella enterica serovar Choleraesuis実験感染, 溝呂木 聖子; 矢ヶ部 陽子; 芝原 友幸; 秋庭 正人; 楠本 正博; 佐久間 晶子; 中條 満; 新開 浩樹; 上西 博英; 宗田 吉広, 日本豚病研究会報, (63) 6 - 11, 2014 02 , Toll-like receptor5(TLR5)は細菌のべん毛タンパク質(flagellin)を認識するパターン認識受容体で、豚ゲノムの解析においてサルモネラのflagellin及びSalmonella enterica serovar Choleraesuis(SC)の認識を低下させるTLR5一塩基多型(single nucleotide polymorphism: SNP) (C1205T)が報告されている。当該SNPがヘテロ型のランドレース豚の雌雄を交配して得られる妊娠母豚の子宮切除術によって作出されたhysterectomy produced colostrum deprived(HPCD)子豚へSCを無菌ミルクに混ぜて2回の経口実験感染を行った結果、HPCD子豚へのSC経口感染により、これまで再現が困難であった下痢や敗血症を特徴とする、豚サルモネラ症の病態モデルの再現ができた。しかし当該TLR5 SNPのSC感染に対する影響については、感染初期での排菌量に有意差は認められず、生存日数への影響についても2回の実験からは確かめられなかった。その理由としてHPCD子豚ではflagellin刺激に対するIL-6応答の顕著な低下が原因である可能性が示唆された。 |
| Analysis of intestinal bacterial flora transplanted from bovine to chicken, 楠本 正博; 岩田 剛敏; 秋庭 正人, 動物衛生研究所研究報告, (121) 31 - 33, 2014 |
| 薬剤耐性アイランドの多コピー化に基づくサルモネラの耐性増強, 李謙一; 稲岡隆史; 楠本正博; 内田郁夫; 岩田剛敏; 岡本晋; 矢部季見子; 秋庭正人, 日本獣医学会学術集会講演要旨集, 156th, 2013 08 30 |
| Salmonella Choleraesuisの離乳子豚に対する感染試験:TLR5の一塩基多型(C1205T)の影響, 宗田吉広; 宗田吉広; 溝呂木聖子; 溝呂木聖子; 矢ケ部陽子; 芝原友幸; 秋庭正人; 楠本正博; 佐久間晶子; 中條満; 新開浩樹; 上西博英, 日本獣医学会学術集会講演要旨集, 156回, 2013 08 |
| カンピロバクター研究の最前線 細胞表面糖鎖の構造変化がカンピロバクター環境抵抗性に及ぼす影響, 岩田 剛敏; 知久 和寛; 天野 憲一; 楠本 正博; 秋庭 正人, 日本細菌学雑誌, 68, (1) 106 - 106, 2013 02 , https://search-tp.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2013&ichushi_jid=J01077&link_issn=&doc_id=20130227320078&doc_link_id=%2Fck0saiki%2F2013%2F006801%2F084%2F0106-0106%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fck0saiki%2F2013%2F006801%2F084%2F0106-0106%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif |
| 細胞表面糖鎖の構造変化がカンピロバクター環境抵抗性に及ぼす影響, 岩田 剛敏; 知久 和寛; 天野 憲一; 楠本 正博; 秋庭 正人, 日本細菌学雑誌, 68, (1) 138 - 138, 2013 02 , https://search-tp.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2013&ichushi_jid=J01077&link_issn=&doc_id=20130227320173&doc_link_id=%2Fck0saiki%2F2013%2F006801%2F179%2F0138-0138%26dl%3D0&url=https%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fck0saiki%2F2013%2F006801%2F179%2F0138-0138%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif |
| カンピロバクターの細胞表面糖脂質の糖鎖欠損により鶏腸管内定着性が低下する, 岩田剛敏; 知久和寛; 天野憲一; 楠本正博; 亀山眞由美; 小野裕嗣; 秋庭正人, 農研機構動物衛生研究所成果情報(Web), 2013, 2013 |
| カンピロバクターの胆汁酸抵抗性及び鶏腸管内定着に重要なLOS糖鎖構造, 岩田 剛敏; 知久 和寛; 天野 憲一; 楠本 正博; 秋庭 正人, 日本獣医学会学術集会講演要旨集, 154回, 2012 08 |
| ブタTLR遺伝子の多型がサルモネラ認識に及ぼす影響の解析, 新開浩樹; 鈴木倫太郎; 秋庭正人; 奥村直彦; 上西博英, 日本畜産学会大会講演要旨, 114回, 2011 08 |
| 近年北海道で分離される牛由来Salmonella Typhimuriumの遺伝学的特徴, 玉村雪乃; 内田郁夫; 田中聖; 秋庭正人; 窪田宜之; 秦英司; 菅野徹; 畠間真一; 石原凉子, 農研機構動物衛生研究所成果情報(Web), 2011, 2011 |
| Salmonella Typhimurium DT104は百日咳毒素様のADP‐リボシル化酵素を産生する, 内田郁夫; 石原涼子; 田中聖; 秦英司; 牧野壮一; 菅野徹; 畠間真一; 木嶋眞人; 秋庭正人; 渡部淳; 窪田宜之, 動物衛生研究成果情報, (9) 39 - 40, 2010 10 29 |
| Avian pathogenic Escherichia coli infection as an extraintestinal pathogenic E. coli infection, 秋庭 正人, 医学のあゆみ, 235, (4) 345 - 350, 2010 10 23 |
| Bactericidal effect of superfine nanobubble and high density ozone water on Campylobacter jejuni, 岩田 剛敏; 秋庭 正人; 高木 昌美, Research report of animal feed, 34, 2009 06 |
| ヒトと動物に共通する腸内細菌のストレス耐性, 中澤 宗生; 秋庭 正人; 佐藤 国雄, 畜産の研究, 62, (2) 252 - 254, 2008 02 , 人獣共通感染性腸内細菌である腸管出血性大腸菌（EHEC）O157を含む志賀毒素産生性大腸菌、サルモネラ、エンテロバクターなどの各種ストレスに対する耐性を調べた。EHEC O157：H7、志賀毒素産生性大腸菌、一般大腸菌はサルモネラやエンテロバクターに比べ高い酸耐性を示した。EHEC O157の酸耐性は一般大腸菌と比較しても有意に高かく、熱耐性でも、他の大腸菌やサルモネラより高い数値を示した。ヒトのEHEC O157：H7の感染菌量は100個未満であり、少量菌で感染が成立する要因の一つとして本菌のもつ強い酸耐性が考えられた。また、サルモネラの熱耐性では、感染宿主域が狭く種特異性が高い血清型Dublin、TyphisuisおよびPullormは熱ストレスに対してまったく抵抗できなかったが、宿主域が広く種特異性の低い血清型Enteritidis、Typhimurium、Agona、Infantis、SenftenbergおよびVirchowは0.001％〜30.0％の生残率を示した。サルモネラ食中毒の主要血清型であるこれら菌型のもつストレス耐性能は、環境や食品中での生存性に関与する要因の一つである考えられた。以上のように、腸内細菌のストレス耐性は菌種よる違いがあるが、強いストレス耐性能を有する菌型が存在することから、これらの特性を考慮した制御法が必要である。 |
| Serotype of Salmonella enterica subsp. enterica sent to our laboratory between 1996-2006, 吉井 紀代; 秋庭 正人; 鮫島 俊哉, Bulletin of the National Institute of Animal Health, (114) 45 - 50, 2007 , 1996～2006年の11年間に動物衛生研究所腸内系細菌ユニット(旧ズーノーシス研究室)に公的機関から送付された動物由来サルモネラの血清型についてまとめた。牛由来19血清型158株，豚由来17血清型100株，鶏由来60血清型605株，その他の動物由来11血清型78株であった。家畜由来サルモネラの主要血清型は1980～1995年送付株と1996～2006年送付株で大部分は共通であった。一方，どの家畜由来株でも1980～1995年よりも1996～2006年の送付株で抗原構造表に記載のない血清型の割合が増加していた。 |
| Salmonella Typhimurium DT104はADP‐ribosyltransferase毒素と相同性を示す遺伝子(artAB)を保持している, 内田郁夫; 西森敬; 田中聖; 牧野壮一; 菅野徹; 石原涼子; 畠間真一; 北野理恵; 木嶋眞人; 鮫島俊哉; 秋庭正人; 中沢宗生; 横溝祐一; 斉藤真理子, 動物衛生研究成果情報, (5) 31 - 32, 2006 08 31 |
| 牛由来サルモネラの薬剤耐性に関する最近の知見, 秋庭正人, 臨床獣医, 24, 2006 |
| 牛由来大腸菌O157:H7に関する分子疫学的研究 (特集 家畜衛生と疫学), 秋庭 正人, 畜産の研究, 54, (10) 1065 - 1072, 2000 10 |
| パルスフィールドゲル電気泳動による腸管出血性大腸菌O157:H7の分子疫学的解析法, 秋庭 正人, 獣医畜産新報, 53, (1) 14 - 21, 2000 01 |
| Fecal Shedding and Serum Antibody Responses of Cattle Experimentally Infected with Enterohaemorrhagic Escherichia coli O157 : H7, AKIBA Masato; SAMEJIMA Toshiya; MIYAZAWA Hiroshi; SHINAGAWA Kunihiro; NAKAZAWA Muneo, 感染症学雑誌 : 日本伝染病学会機関誌 : the journal of the Japanese Association for Infectious Diseases, 73, (10) 1082 - 1083, 1999 10 20 |
| Carrier State of Escherichia coli O157 : H7 in Cattle, AKIBA Masato; SAMEJIMA Toshiya; NAKAZAWA Muneo, 感染症学雑誌 : 日本伝染病学会機関誌 : the journal of the Japanese Association for Infectious Diseases, 72, (2) 167 - 168, 1998 02 20 |
| Serotype of Salmonella choleraesuis subsp. Choleraesuis isolated from domestic animals., 秋庭正人; 大宅辰夫; 三森真琴; 鮫島俊哉; 中沢宗生, 農林水産省家畜衛生試験場研究報告, (102/103) 43 - 48, 1996 10 |
Research Grants & Projects
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Nation-wide spatio-temporal phylogenomics of E. coli O157 and search for high risk O157 lineages, 20160401, 20190331, Hayashi Tetsuya; Ogura Yoshitoshi; IYODA Sunao; KUSUMOTO Masahiro, We sequenced 2,354 clinical O157 strains isolated in 2005, 2010, 2015 in Japan, which covered most strains isolated in each year in Japan, and 271 strains that were isolated from bovine between 1996 and 2017 in 19 prefectures in Japan. Using the core SNP information obtained from these draft sequences, we performed high-resolution phylogenetic analysis and identified several sublineages dominating in Japan and the turnovers of the dominant clones or sublineages. By developing a novel Stx2 subtyping method, we identified the difference in dominant subtype between sublineages and subtype change in several sublineages. We are now performing more detailed analyses, including analysis of sublineage specific genes and integrated analyses of clinical and bacterial genome information to identify high-risk O157 clones or sublineages and their dynamics. |
| Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research, Amplification of a novel chromosomal resistance island of Salmonella Typhimurium confers increased resistance to antimicrobials, 20130401, 20160331, AKIBA Masato; KURODA Makoto, We found that antimicrobial resistance of Salmonella Typhimurium increased by gene amplification of a novel chromosomal resistance island (GI). Copy number of GI increased up to 85 and the elevation was accompanied by increased amount of transcription. The amplification occurred at the original position. In some cases, the transposition of part of GI occurred after insertion of IS26 or IS1, leading to the amplification of GI at different position. Elevation of transcription of SOS response related genes was observed in a comprehensive gene expression analysis. |
| 日本学術振興会, 科学研究費助成事業, インドにおける医薬品及び薬剤耐性菌環境汚染調査, 20120401, 20160331, グルゲ キールティ・シリ; 山下 信義; 秋庭 正人, 2014年にチェンナイより採集した河川水・底質試料について医薬品及び有害化学物質の分析を行った。先進国では過去50年以上にわたって汚染が進行している有機過フッ素化合物や有機シロキサンについては、インドの汚染は始まったばかりであることが柱状底質試料を用いた歴史的復元により明らかとなった。医薬品ではイブプロフェンの濃度が最も高く、薬剤耐性菌への関与が疑われるキノロン剤のオフロキサシン濃度が次いで高濃度であった。 また、同じ底質試料の変異原性強度の空間的と鉛直分布を、コメットアッセイを用いて、多環芳香族炭化水素（PAHs）、およびそのハロゲン化物を指標として判定した。チェンナイ市・クーム川の底質は石油起源のPAHsによる高度な汚染が検証された。底質の変異原性の強さは、PAHsの汚染度に関連していた。また、４種類の下水汚泥の抽出物を雄性マウスに摂取させ、肝臓の遺伝子発現の変化をマイクロアレイ解析した。200以上の遺伝子について、それぞれの下水処理施設に特有な異なる誘導が見いだされ、排水の性質は排出元に大きく影響されることが示唆された。 さらに、これまでに分離した446株の大腸菌のうち、セフォタキシムやイミペネムといった人の治療に汎用されるβ-ラクタム剤に耐性を示す169株（38%）の薬剤耐性因子等を詳細に解析した。12の抗菌剤に対する薬剤感受性を調べたところ2剤を除いて50%以上の耐性菌分布率を示した。また、全ての菌は3剤以上に耐性を示し、最も分布率が高かったのは10剤耐性菌（25%）であった。β-ラクタマーゼ遺伝子型別のうち、CTX-M型の分布率が66%と最も高く、次いでTEM、OXA、CIT型の分布率がそれぞれ約40%であった。無作為に選んだ36の環境水サンプルに由来する49プラスミドの全塩基配列を解読し、β-ラクタマーゼ遺伝子を含む各プラスミドの系統と薬剤耐性遺伝子の分布を明らかにした。 |
| 日本学術振興会, 科学研究費助成事業, わが国の動物由来サルモネラにおけるセフェム系抗菌剤耐性に関する分子遺伝学的研究, 2010, 2011, 秋庭 正人; PETERFRANCIS SHAHADA; PETER FRANCIS Shahada, 抗菌剤が使用される家畜の生産現場は薬剤耐性菌選択の場の一つと考えられている。家畜由来薬剤耐性菌がヒトに感染した場合、その治療を難しくする可能性があるため、家畜由来食中毒菌の薬剤耐性化は公衆衛生上、看過できない。とりわけ、ヒトの治療に汎用される広域セファロスポリン(ESC)に対する耐性菌の出現には注意を払う必要がある。 2004年以降、北海道の牛においてESCに耐性を示す特定遺伝子型のSalmonella Typhimurium(ST-VII-6)が分離されている。本研究では、こめESC耐性が染色体上の多剤耐性ゲノミックアイランドの一つであるGI-VII-6に規定されていることを明らかにした。GI-VII-6の全長は125kbでESC耐性を規定するCMY-2 β-ラクタマーゼ遺伝子の他、10の薬剤耐性遺伝子を含んでおり、その塩基配列の特徴から薬剤耐性プラスミドが染色体上に転移したものと推察された。解析した22株のST-VII-6は全てGI-VII-6を保有しており、ユニークなSalmonella Typhimurium(ST)クローンが北海道の牛に拡散したものと推察された。 また、1農場で発生した牛サルモネラ症から薬剤耐性パターンの異なるST 4株が分離されたので諸性状を解析したところ、プラスミドプロファイルに違いは認められるものの、由来は同一の菌株で農場内において保有プラスミドが変化したものと推察された。薬剤耐性パターンは保有プラスミドによるため菌株の遺伝子型による近縁関係を反映するとは限らないことが示された。 さらに南九州地域のブロイラーからESCに耐性を示すサルモネラ血清型Infantis、Manhattan、および大腸菌を分離し、菌種内および菌種間の薬剤耐性プラスミド伝達の有無を調べたところ、両菌種内で薬剤耐性プラスミドの交換が起こっている可能性を指摘することができた。加えて、これらプラスミドが実験的に他菌種に伝達可能であることを示した。 |
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