School of Veterinary Medicine, Department of Veterinary Medicine
萩原克郎 ハギワラ カツロウ
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Last Update :2018/11/09

Researcher Profile and Settings

Name

HAGIWARA Katsuro

Affiliation (Master)

School of Veterinary Medicine, Department of Veterinary Medicine

Affiliation & Job

School of Veterinary Medicine, Department of Veterinary Medicine
Professor

Degree

(BLANK)

Research Activities

Research Areas

Zootechnical science/Veterinary medical science, Basic veterinary science/Basic zootechnical science

Research Interests

Virology, Intestinal microbiota, Immunity, Tumor immunity,
Veterinary Microbiology,

Published Papers

Influence of changes in the intestinal microflora on the immune function in mice., Kishida S, Kato-Mori Y, Hagiwara K, The Journal of veterinary medical science, 80, 3, 440, 446, 20180200, Refereed
Socio-economic factors associated with voluntary rabies control measures in Vietnam., Kadowaki H, Duc PP, Sato K, Phuong PTM, Hagiwara K, Makita K, Preventive veterinary medicine, 157, 105, 114, 20180901, Refereed
Bacteriophage ΦSA012 Has a Broad Host Range against Staphylococcus aureus and Effective Lytic Capacity in a Mouse Mastitis Model., Iwano H, Inoue Y, Takasago T, Kobayashi H, Furusawa T, Taniguchi K, Fujiki J, Yokota H, Usui M, Tanji Y, Hagiwara K, Higuchi H, Tamura Y, Biology, 7, 1, 20180100, Refereed
Survey of tick-borne zoonotic viruses in wild deer in Hokkaido, Japan., Uchida L, Hayasaka D, Ngwe Tun MM, Morita K, Muramatsu Y, Hagiwara K, The Journal of veterinary medical science, 80, 6, 985, 988, 20180600, Refereed
Determining the Frequency of Dry Lake Bed Formation in Semi-Arid Mongolia From Satellite Data, Demura Yuta, Hoshino Buho, Baba Kenji, McCarthy Christopher, Sofue Yuki, Kai Kenji, Purevsuren Tsedendamba, Hagiwara Katsuro, Noda Jun, LAND, 6, 4, 20171200, Refereed
Zoonotic Enterobacterial Pathogens Detected in Wild Chimpanzees., McLennan MR, Mori H, Mahittikorn A, Prasertbun R, Hagiwara K, Huffman MA, EcoHealth, 15, 1, 143, 147, 20171100, Refereed
Regulation of Constitutive Interferon-Stimulated Genes (Isgs) in Tumor Cells Contributes to Enhanced Antitumor Response of Newcastle Disease Virus-Infected Tumor Vaccines., Takamura-Ishii M, Nakaya T, Hagiwara K, Cancers, 10, 6, 20180600, Refereed
Problems in the Protection of Reintroduced Przewalski's Horses (Equus ferus przewalskii) Caused by Piroplasmosis., Tarav M, Tokunaga M, Kondo T, Kato-Mori Y, Hoshino B, Dorj U, Hagiwara K, Journal of wildlife diseases, 53, 4, 911, 915, 20170700, Refereed
Induction of antitumor response to fibrosarcoma by Newcastle disease virus-infected tumor vaccine., Takamura-Ishii M, Miura T, Nakaya T, Hagiwara K, Medical oncology (Northwood, London, England), 34, 10, 171, 20170900, Refereed
Postnatal changes in the relative abundance of intestinal Lactobacillus spp. in newborn calves., Takino T, Kato-Mori Y, Motooka D, Nakamura S, Iida T, Hagiwara K, The Journal of veterinary medical science, 79, 3, 452, 455, 20170300, Refereed
Neutralizing activities against seasonal influenza viruses in human intravenous immunoglobulin., Onodera H, Urayama T, Hirota K, Maeda K, Kubota-Koketsu R, Takahashi K, Hagiwara K, Okuno Y, Ikuta K, Yunoki M, Biologics : targets & therapy, 11, 23, 30, 20170000, Refereed
Sero-epidemiological analysis of vertical transmission relative risk of Borna disease virus infection in dairy herds., Ando T, Takino T, Makita K, Tajima M, Koiwa M, Hagiwara K, The Journal of veterinary medical science, 78, 11, 1672, 1672, 20161200, Refereed
Change in the responsiveness of interferon-stimulated genes during early pregnancy in cows with Borna virus-1 infection., Takino T, Okamura T, Ando T, Hagiwara K, BMC veterinary research, 12, 1, 253, 20161114, Refereed
Phage Therapy Is Effective in a Mouse Model of Bacterial Equine Keratitis., Furusawa T, Iwano H, Hiyashimizu Y, Matsubara K, Higuchi H, Nagahata H, Niwa H, Katayama Y, Kinoshita Y, Hagiwara K, Iwasaki T, Tanji Y, Yokota H, Tamura Y, Applied and environmental microbiology, 82, 17, 5332, 5339, 20160900, Refereed
Hepatitis E virus derived from different sources exhibits different behaviour in virus inactivation and/or removal studies with plasma derivatives., Yunoki M, Tanaka H, Takahashi K, Urayama T, Hattori S, Ideno S, Furuki R, Sakai K, Hagiwara K, Ikuta K, Biologicals : journal of the International Association of Biological Standardization, 44, 5, 403, 411, 20160900, Refereed
Isolation and characterization of a novel Rhabdovirus from a wild boar (Sus scrofa) in Japan., Sakai K, Hagiwara K, Omatsu T, Hamasaki C, Kuwata R, Shimoda H, Suzuki K, Endoh D, Nagata N, Nagai M, Katayama Y, Oba M, Kurane I, Saijo M, Morikawa S, Mizutani T, Maeda K, Veterinary microbiology, 179, 3-4, 197, 203, 20150900, Refereed
Viral detection by high-throughput sequencing., Motooka D, Nakamura S, Hagiwara K, Nakaya T, Methods in molecular biology (Clifton, N.J.), 1236, 125, 134, 20150000, Refereed
The influence of Borna disease viral infection on dairy cow reproduction., Hagiwara K, Ando T, Koiwa M, The Journal of veterinary medical science, 74, 4, 419, 421, 20120400, Refereed
Hepatitis E virus in Norway rats (Rattus norvegicus) captured around a pig farm., Kanai Y, Miyasaka S, Uyama S, Kawami S, Kato-Mori Y, Tsujikawa M, Yunoki M, Nishiyama S, Ikuta K, Hagiwara K, BMC research notes, 5, 4, 20120100, Refereed
Changes in acute-phase proteins and cytokines in serum and milk whey from dairy cows with naturally occurring peracute mastitis caused by Klebsiella pneumoniae and the relationship to clinical outcome., Hisaeda K, Arima H, Sonobe T, Nasu M, Hagiwara K, Kirisawa R, Takahashi T, Kikuchi N, Nagahata H, The Journal of veterinary medical science, 73, 11, 1399, 1404, 20111100, Refereed
Interleukin-6 in quarter milk as a further prediction marker for bovine subclinical mastitis., Sakemi Y, Tamura Y, Hagiwara K, The Journal of dairy research, 78, 1, 118, 121, 20110200, Refereed
Fermentation metabolites from Lactobacillus gasseri and Propionibacterium freudenreichii exert bacteriocidal effects in mice., Kato-Mori Y, Orihashi T, Kanai Y, Sato M, Sera K, Hagiwara K, Journal of medicinal food, 13, 6, 1460, 1467, 20101200, Refereed
Significant neutralizing activity of human immunoglobulin preparations against pandemic 2009 H1N1., Yunoki M, Kubota-Koketsu R, Urayama T, Sasaki T, Analiwa D, Konoshima Y, Ideno S, Fukunaga Y, Morikawa S, Hiroi S, Takahashi K, Okuno Y, Hagiwara K, Ikuta K, British journal of haematology, 148, 6, 953, 955, 20100300, Refereed
Leptin inhibits mitogen-induced proliferation of peripheral T lymphocytes from Holstein cows., Ahmed M, Kimura K, Soliman M, Yamaji D, Okamatsu-Ogura Y, Ishioka K, Makondo K, Hagiwara K, Saito M, Veterinary journal (London, England : 1997), 176, 3, 361, 368, 20080600, Refereed
Characterization of a Borna disease virus field isolate which shows efficient viral propagation and transmissibility., Watanabe Y, Ibrahim MS, Hagiwara K, Okamoto M, Kamitani W, Yanai H, Ohtaki N, Hayashi Y, Taniyama H, Ikuta K, Tomonaga K, Microbes and infection, 9, 4, 417, 427, 20070400, Refereed
Molecular characterization and functional expression of equine interleukin-1 type I and type II receptor cDNAs., Kirisawa R, Hashimoto N, Tazaki M, Yamanaka H, Ishii R, Hagiwara K, Iwai H, Veterinary immunology and immunopathology, 109, 3-4, 219, 231, 20060200, Refereed
The role of IFN-gamma in cattle infected with Theirelia sergenti., Hagiwara K, Tokuda M, Baba T, Yamanaka H, Kirisawa R, Tsuji M, Ishihara C, Iwai H, Veterinary parasitology, 127, 2, 105, 110, 20050100, Refereed
Changes in interleukin-6 concentration in peripheral blood of pre- and post-partum dairy cattle and its relationship to postpartum reproductive diseases., Ishikawa Y, Nakada K, Hagiwara K, Kirisawa R, Iwai H, Moriyoshi M, Sawamukai Y, The Journal of veterinary medical science, 66, 11, 1403, 1408, 20041100, Refereed
Epizootiologic survey for Babesia microti among small wild mammals in northeastern Eurasia and a geographic diversity in the beta-tubulin gene sequences., Zamoto A, Tsuji M, Wei Q, Cho SH, Shin EH, Kim TS, Leonova GN, Hagiwara K, Asakawa M, Kariwa H, Takashima I, Ishihara C, The Journal of veterinary medical science, 66, 7, 785, 792, 20040700, Refereed
Transient detection of proinflammatory cytokines in sera of colostrum-fed newborn calves., Yamanaka H, Hagiwara K, Kirisawa R, Iwai H, The Journal of veterinary medical science, 65, 7, 813, 816, 20030700, Refereed
Proinflammatory cytokines in bovine colostrum potentiate the mitogenic response of peripheral blood mononuclear cells from newborn calves through IL-2 and CD25 expression., Yamanaka H, Hagiwara K, Kirisawa R, Iwai H, Microbiology and immunology, 47, 6, 461, 468, 20030000, Refereed
Cytokine profile of a Holstein calf with bovine leukocyte adhesion deficiency during the acute-phase inflammatory response., Nagahata H, Hagiwara K, Kasamatsu M, Higuchi H, Kurosawa T, The Journal of veterinary medical science, 64, 12, 1141, 1143, 20021200, Refereed
Analysis of serum cytokine profile in a holstein heifer with leukocyte adhesion deficiency which survived for long period., Nagahata H, Hagiwara K, Higuchi H, Kirisawa R, Iwai H, The Journal of veterinary medical science, 64, 8, 683, 687, 20020800, Refereed
Nosological study of Borna disease virus infection in race horses., Hagiwara K, Okamoto M, Kamitani W, Takamura S, Taniyama H, Tsunoda N, Tanaka H, Iwai H, Ikuta K, Veterinary microbiology, 84, 4, 367, 374, 20020200, Refereed
Meriones meridianus and Lagurus lagurus as alternative definitive hosts of Echinococcus multilocularis and E. granulosus., Oku Y, Wei J, Chai JJ, Osman I, Wei J, Liao LF, Asakawa M, Hagiwara K, Kobayashi K, Ito M, Experimental animals, 51, 1, 27, 32, 20020100, Refereed

Conference Activities & Talks

reatment of JAK inhibitor improve the infectivity to tumor and immune response by Newcastle disease virus-infected tumor vaccine, ○Mai Ishii, Takaaki Nakaya, Katsuro Hagiwara,   2017 10
Anti-viral effect of Lactobacilli on Influenza A (H1N1) virus infection., Yuko Kato-Mori, Shigefumi Kishida, Minoru Okamoto, and Katsuro Hagiwara, International Union of Microbiological Societies Congresses,   2017 06

Misc

呼吸の病態生理学 第51回 呼吸器疾患の臨床微生物学 2:呼吸器疾患関連ウイルスによる発症機序(後編), 萩原克郎, 鈴木一由, 加藤敏英, 臨床獣医, 34,   2016 10 01 , http://jglobal.jst.go.jp/public/201602292192288446
Silage production using Lactobacillus plantarum which separated from the natural world, 石井智美, 森ゆうこ, 萩原克郎, 酪農学園大学紀要 自然科学編(Web), 41,   2016 10 , http://jglobal.jst.go.jp/public/201602217359111392
道内で特用家畜・家禽として飼育されるアルパカ(Vicugna pacos)とエミュー(Dromaius novaehollandiae)の寄生虫保有状況に関する予備試験, 高野結衣, 竹内萌香, 立本完吾, 萩原克郎, 浅川満彦, 北海道獣医師会雑誌, 60,   2016 09 10 , http://jglobal.jst.go.jp/public/201602257790438430
呼吸の病態生理学 第50回 呼吸器疾患の臨床微生物学 1:呼吸器疾患関連ウイルスによる発症機序(前編), 萩原克郎, 鈴木一由, 加藤敏英, 臨床獣医, 34,   2016 09 01 , http://jglobal.jst.go.jp/public/201602214653251230
エゾシカにおけるE型肝炎ウイルス感染の疫学調査, 萩原克郎, 高橋万世, 加藤(森)ゆうこ, 加藤(森)ゆうこ, 岡本実, 浅川満彦, 日本獣医学会学術集会講演要旨集, 159th,   2016 08 30 , http://jglobal.jst.go.jp/public/201602216726879762
採卵鶏3種におけるワクチン応答の違いについて, 中村優臣, 瀧野祥司, 瀧野祥司, 峯苫健太, 加藤(森)ゆうこ, 萩原克郎, 北海道獣医師会雑誌, 60,   2016 08 10 , http://jglobal.jst.go.jp/public/201602269636157263
大気ブラックカーボン及びその役割を知る, 安成哲平, 兼保直樹, 能田淳, 星野仏方, 野口泉, 村尾直人, 秋山雅行, 藤岡真路, 馬場賢治, 萩原克郎, 甲斐憲次, 山口高志, 鈴木啓明, 松本利恵, 細氷(Web),   2016 , http://jglobal.jst.go.jp/public/201802267117231037
北海道内乳牛におけるボルナ病ウイルスの感染状況の把握, 小林洋平, 近藤達成, 加藤(森)ゆうこ, 安藤達哉, 小岩政照, 萩原克郎, 北海道獣医師会雑誌, 59,   2015 08 10 , http://jglobal.jst.go.jp/public/201502211864098666
乳牛におけるボルナ病ウイルス母子感染の分析疫学的相対リスク, 安藤達哉, 小林洋平, 近藤達成, 蒔田浩平, 田島誉士, 萩原克郎, 小岩政照, 北海道獣医師会雑誌, 59,   2015 08 10 , http://jglobal.jst.go.jp/public/201502206183800148
競走馬の細菌性角膜炎に対するファージセラピーの有効性, 古澤貴章, 岩野英知, 樋口豪紀, 萩原克郎, 丹羽秀和, 田村豊, 感染症学雑誌, 89,   2015 03 20 , http://jglobal.jst.go.jp/public/201502212881115120
牛初乳中の細菌叢と利用方法の検討, 高橋沙季, 武井誠子, 石井智美, 萩原克郎, 栄養学雑誌, 72,   2014 08 20 , http://jglobal.jst.go.jp/public/201402236455417002
生乳(初乳)中にLactobacillus plantarumを入れて発酵した際の細菌叢の変化, 武井誠子, 高橋沙季, 石井智美, 萩原克郎, 栄養学雑誌, 72,   2014 08 20 , http://jglobal.jst.go.jp/public/201402255704783221
競走馬の細菌性角膜炎に対するファージセラピーの検討, 岩野英知, 冷清水雄太郎, 古澤貴章, 樋口豪紀, 永幡肇, 萩原克郎, 丹羽秀和, 片山芳也, 木下優太, 横田博, 田村豊, 北海道獣医師会雑誌, 58,   2014 08 10 , http://jglobal.jst.go.jp/public/201402288695854604
マウス馴化vCJD株を用いた血漿分画製剤工程のプリオン除去, 上平崇, 久保純, 大久保祐士, 坂井薫, 加藤(森)ゆうこ, 萩原克郎, 生田和良, 柚木幹弘, 血液事業, 37,   2014 08 01 , http://jglobal.jst.go.jp/public/201402204544946419
大脳萎縮が顕著に認められたボルナ病ウイルス抗体陽性の猫の1例, 坂大智洋, 新野猛, 萩原克郎, 二瓶和美, チェンバーズ ジェームズ, 内田和幸, 獣医神経病学会, 40th,   2014 07 05 , http://jglobal.jst.go.jp/public/201502265315208532
プロバイオティクスとプレバイオティクス~プレバイオティクスとしての発酵代謝産物の利用~, 加藤(森)ゆうこ, 安井勝巳, 後藤正光, 萩原克郎, 宮城県獣医師会会報, 67,   2014 04 , http://jglobal.jst.go.jp/public/201702222511586476
Lactobacillus plantarum投与マウスにおける抗炎症効果の検討, 加藤(森)ゆうこ, 萩原克郎, 日本細菌学雑誌, 69,   2014 02 25 , http://jglobal.jst.go.jp/public/201402299813946154
北海道日高地方におけるエゾシカ(Cervus nippon yesoensis)の内部寄生虫相および道内エゾシカ寄生日本産肝蛭(Fasciola sp.)の分布域について, 森昇子, 三觜慶, 鈴木瑞穂, 萩原克郎, 浅川満彦, 北海道獣医師会雑誌, 58, (2) 44 - 47,   2014 02 10 , http://jglobal.jst.go.jp/public/201402244380515022
由来の異なるE型肝炎ウイルスの熱感受性の違いについて, 高橋一惠, 大久保祐士, 古木理恵, 服部眞次, 浦山健, 坂井薫, 柚木幹弘, 萩原克郎, 生田和良, 血液事業, 36, (3) 679 - 685,   2013 11 01 , http://jglobal.jst.go.jp/public/201402267579352769
血液製剤の安全対策―プリオン除去の現状―, 加藤(森)ゆうこ, 柚木幹弘, 生田和良, 萩原克郎, モダンメディア, 59, (9) 231 - 237,   2013 09 , http://jglobal.jst.go.jp/public/201302219531888142
高淘汰更新牛群におけるボルナ病ウイルスの感染状況と抗体陽性牛の母系血統的考察, 安藤達哉, 小岩政照, 萩原克郎, 及川伸, 太田浩運, 篠田浩二郎, 山本康朗, 矢崎美恵, 染谷勇介, 大野和吉, 藪崎尚弘, 阿部竜大, 北海道獣医師会雑誌, 57,   2013 08 10 , http://jglobal.jst.go.jp/public/201302275730263728
知床半島を中心に生息するエゾシカCervus nippon yesoensisから見出された外部寄生虫性昆虫2種の記録, 水主川剛賢, 石名坂豪, 増田泰, 小川人士, 高須恵美, 森昇子, 萩原克郎, 浅川満彦, 知床博物館研究報告, (35) 11 - 14,   2013 05 21 , http://jglobal.jst.go.jp/public/201402278195919658
Case Reports on Two Species of Parasitic Insects Obtained from Sika Deer Cervus nippon yesoensis in Shiretoko Peninsula and Its Proximity, Hokkaido, Japan, 水主川 剛賢, 石名坂 豪, 増田 泰, 小川 人士, 高須 恵美, 森 昇子, 萩原 克郎, 浅川 満彦, 知床博物館研究報告, (35) 11 - 14,   2013 05 , http://ci.nii.ac.jp/naid/120005761888, Case reports on two species of parasitic insects including Solenopotes sp. cf. binipilosus (Linognathidae: Anoplura) and Lipoptera fortisetosa (Hippoboscidae: Diptera) obtained from sika deer Cervus nippon yesoensis in Shiretoko Peninsula and its proximity, Hokkaido, Japan, were presented. Parasitism of Solenopotes sp. cf. binipilosus on C. nippon was first established, although a record of Solenopotes sp. without any morphological data was presented in the other part of Hokkaido in the 1990's. Photographs of both insect species and their brief epidemiological comments are given.
宮城県石巻市津波被災地域における環境リスクおよび仮設住宅居住者の現状, 蒔田浩平, 伊下一人, 渡辺洋子, 茅野大志, 萩原克郎, 浅川満彦, 小川健太, 能田淳, 佐々木均, 中谷暢丈, 樋口豪紀, 岩野英知, 田村豊, Journal of Epidemiology, 23,   2013 01 24 , http://jglobal.jst.go.jp/public/201502253576358126
機能性飼料投与による新生子牛の免疫機能変化, 和田雄治, 岡村卓, 加藤(森)ゆうこ, 萩原克郎, 北海道獣医師会雑誌, 56,   2012 08 10 , http://jglobal.jst.go.jp/public/201202288151910107
E型肝炎ウイルス(HEV)の熱感受性の違いについて, 高橋一恵, 大久保祐士, 古木理恵, 服部眞次, 浦山健, 坂井薫, 柚木幹弘, 萩原克郎, 生田和良, 血液事業, 35,   2012 08 01 , http://jglobal.jst.go.jp/public/201202298709979937
HEV mini‐pool‐NAT法確立とベネシスにおける原料血漿の試験成績, 皆木隆男, 井手野祥次, 上田千晶, 高橋一恵, 浦山健, 服部眞次, 坂井薫, 柚木幹弘, 萩原克郎, 生田和良, 血液事業, 35,   2012 08 01 , http://jglobal.jst.go.jp/public/201202207553492670
宮城県石巻市津波被災地域における環境リスクの評価, 蒔田浩平, 伊下一人, 茅野大志, 萩原克郎, 浅川満彦, 小川健太, 能田淳, 佐々木均, 中谷暢丈, 樋口豪紀, 岩野英知, 田村豊, 獣医疫学雑誌, 16,   2012 07 20 , 10.2743/jve.16.9, http://jglobal.jst.go.jp/public/201202245200355113
A Case of Preliminary Utilization of Commercial ELISA Kit for Detecting Excretion of Urinary 8-hydroxyguanosine (8-OHdG) in Captive Chimpanzees, Pan troglodytes, 大島由子, 水尾愛, 洲鎌圭子, 伊谷原一, 志村良治, 上林亜紀子, 高橋悟, 大沼学, 翁長武紀, 萩原克郎, 浅川満彦, 動物園水族館雑誌, 52, (4) 140 - 144,   2012 04 25 , http://jglobal.jst.go.jp/public/201202263046002995
Characteristics and prognosis of dairy cows with naturally occurring peracute Escherichia coli and Klebsiella pneumoniae mastitis, 久枝 啓一, 今村 智子, 園部 隆久, 杉山 美恵子, 那須 正信, 永幡 肇, 家畜衛生学雑誌, 37, (4) 121 - 130,   2012 03 , http://ci.nii.ac.jp/naid/120005761798, This study was conducted to evaluate the characteristics and prognosis of dairy cows with naturally occurring peracute Escherichia coli (E. coli) and Klebsiella pneumoniae (K. Pneumoniae) mastitis on dairy farms. Clinical findings, concentrations of acute-phase proteins and cytokines in serum and milk from dairy cows with naturally occurring coliform mastitis were evaluated. The classification values for K. pneumoniae peracute mastitis of dairy cows were respiratory rates of 40 beats/min, blood urea nitrogen (BUN) 19 mg/di, haptoglobin (Hp) in serum and milk of 2000 μg/ml and score >2. The values for total score 8, BUN17mg/dl, serum Hp2000 μg/ml and milk Hp1000 μg/ml and score for prognosis > 2 were considered to be fatal by E. coli peracute mastitis. The values for total score 8, BUN22mg/dl, serum Hp2030 μg/ml, and milk Hp2050 μg/ml and prognosis score >3 were considered to be fatal in K. pneumoniae mastitis. The evaluation of dairy cows with peracute coliform-mastitis by scoring selective measures employed may be valuable for estimating pathogen causing coliform mastitis and its prognosis.
札幌市内のペットショップで販売されていたトカゲ類の寄生虫保有状況, 岩尾一, 篠田理恵, 吉田宗則, 吉田宗則, 萩原克郎, 浅川満彦, 北海道獣医師会雑誌, 56, (1) 5 - 7,   2012 01 10 , http://jglobal.jst.go.jp/public/201202216567278425
An experiment to measure the bowel movement and lipid metabolism of female students who drank 200ml Trial Fermented Milk (TFM) for two trial periods each lasting 21 days., 石井智美, 堀江雅美, 川見祥代, 森ゆうこ, 萩原克郎, 酪農学園大学紀要 自然科学編(Web), 36,   2011 10 , http://jglobal.jst.go.jp/public/201202290807422664
An experiment to measure the bowel movement and lipid metabolism of female students who drank 200 ml Trial Fermented Milk (TFM) for two trial periods each lastins 21 days., ISHII Satomi, HORIE Masami, KAWAMI Sathiyo, MORI Yuuko, HAGIWARA Katuro, Journal of the College of Dairying. Natural science, 36, (1) 67 - 73,   2011 10 , http://ci.nii.ac.jp/naid/110008678193
野鳥で観察された高病原性鳥インフルエンザの臨床所見および簡易検査における注意点, 渡辺有希子, 亀ケ谷千尋, 齊藤慶輔, 萩原克郎, 北海道獣医師会雑誌, 55,   2011 08 10 , http://jglobal.jst.go.jp/public/201102295840725766
マウス馴化型vCJDを用いた工程評価系;作用機序が異なる3種類のろ過膜のプリオン除去能評価, 久保純, 大久保祐士, 中川典子, 川見祥代, 坂井薫, 柚木幹弘, 萩原克郎, 生田和良, 血液事業, 34,   2011 08 01 , http://jglobal.jst.go.jp/public/201102285493397200
バイオ医薬品におけるプリオンの問題―ヒト赤血球を原料とする人工酸素運搬体をめぐる問題―, 柚木幹弘, 萩原克郎, 生田和良, 人工血液, 18, (4) 142 - 150,   2011 03 30 , http://jglobal.jst.go.jp/public/201102263905628110
知っておきたい!ボルナ病 ボルナ病ウイルスの疫学―家畜から野生動物まで―, 萩原克郎, 臨床獣医, 29,   2011 02 01 , http://jglobal.jst.go.jp/public/201102248323283348
E型肝炎ウイルスRNAの核酸増幅検査(NAT)用参照パネルの作製, 内田恵理子, 岡田義昭, 水澤左衛子, 柚木幹弘, 辻川宗男, 皆木隆男, 稲田耕一, 小西久郎, 五十嵐正志, 鈴木光, 嘉悦洋, 下瀬克郎, 萩原克郎, 安江博, 生田和良, 鈴木和博, 山口照英, 生化学,   2011 , http://jglobal.jst.go.jp/public/201202283172617203
Influenza A/H1N1 virus(seasonal/pandemic)に対する人免疫グロブリン製剤の抗体価, 井手野祥次, 浦山健, 辻川宗男, 柚木幹弘, 萩原克郎, 高橋和郎, 奥野良信, 生田和良, 感染症学雑誌, 84, (6) 813 - 814,   2010 11 20 , http://jglobal.jst.go.jp/public/201002219745619082
A型インフルエンザ感染マウスモデルにおける人免疫グロブリン製剤の投与効果, 小林不二夫, 萩原克郎, 浦山健, 柏原純一, 柚木幹弘, 高橋和郎, 奥野良信, 生田和良, 感染症学雑誌, 84,   2010 11 20 , http://jglobal.jst.go.jp/public/201002297550708006
動物の感染症から学ぶ Vol.5 E型肝炎ウイルス―食品媒介性の人獣共通感染症, 萩原克郎, 医学のあゆみ, 234, (12) 1131 - 1136,   2010 09 18 , http://jglobal.jst.go.jp/public/201002258908614321
Hepatitis E virus, 萩原 克郎, 医学のあゆみ, 234, (12) 1131 - 1136,   2010 09 18 , http://ci.nii.ac.jp/naid/40017273874
Relationship between hypocalcemia and cytokines in peracute mastitis of dairy cattle caused by coliform bacteria, 久枝 啓一, 今村 智子, 有馬 春樹, Journal of livestock medicine, 57, (6) 371 - 376,   2010 06 , http://ci.nii.ac.jp/naid/40017170476
反芻家畜における血液代替物としての初乳, 萩原克郎, 人工血液, 18, (1) 9 - 14,   2010 05 20 , http://jglobal.jst.go.jp/public/201002217712122430
Lactobacillus gasseriおよびPropionibacterium freudenreichiiの発酵代謝産物による自然免疫賦活化作用, 加藤(森)ゆうこ, 折橋毅典, 世良健司, 萩原克郎, 腸内細菌学雑誌, 24,   2010 04 , http://jglobal.jst.go.jp/public/201002255757754240
獣医医療における血液代替物の意義, 萩原克郎, 人工血液, 17,   2009 09 16 , http://jglobal.jst.go.jp/public/200902288071463678
黒毛和種牛および半血種馬にみられたボルナ病抗体陽性の2症例, 福永成己, 小松勝一, 大場光洋, 大西光徳, 渡邊敬文, 小岩正照, 萩原克郎, 北海道獣医師会雑誌, 53,   2009 08 01 , http://jglobal.jst.go.jp/public/200902271953078037
Lactobacillus gasseriおよびPropionibacterium freudenreichiiの発酵代謝産物混合剤がマウスにおけるリステリア菌排菌能に及ぼす影響, 加藤(森)ゆうこ, 折橋毅典, 佐藤倫子, 金井祐太, 世良健司, 萩原克郎, 腸内細菌学雑誌, 23,   2009 04 , http://jglobal.jst.go.jp/public/200902286153904114
Preliminary Research on the Excretion of Urinary 8-Hydroxyguanosine (8-OHdG) as a Marker of Protozoan Parasites Infection in Captive Western Lowland Gorillas (Gorilla gorilla gorilla), MIZUO Ai, OHSHIMA Yuko, IMANISHI Ryo, KITADA Yuji, KASAHARA Michiko, HASHIZAKI Fumitaka, WADA Seitaro, MATUNAGA Masayuki, TAKAI Susumu, ONUMA Manabu, ONAGA Takenori, HAGIWARA Katsuro, SANADA Yoshinori, ASAKAWA Mitsuhiko, Jpn J Zoo Wildl Med, 14, (1) 77 - 80,   2009 03 01 , http://ci.nii.ac.jp/naid/110007225779, Urinary 8-hydroxyguanosine (8-OHdG), a common biomarker of oxidative stress, was measured in 9 captive western lowland gorillas (Gorilla gorilla gorilla) by ELISA kit. The data showed that urinary 8-OHdG ranged from 4.3 to 193.1ng/mg creatinine. An individual range of median value was 6.8-52.4ng/mg creatinine. No statistically significant effect was found for infection of protozoan parasites (Balantidium coli and Isospora sp.) without any symptoms (>0.05).
牛の内科実習 第76回 黒毛和種牛のボルナ病, 染谷勇介, 小岩政照, 鈴木一由, 田口清, 萩原克郎, 福永成己, 臨床獣医, 26, (10) 46 - 50,   2008 10 01 , http://jglobal.jst.go.jp/public/200902259635265359
母牛の産歴における黒毛和種子牛の免疫機能の比較, 中山哲也, 田原口貞生, 上野大作, 加藤(森)ゆうこ, 折橋毅典, 萩原克郎, 北海道獣医師会雑誌, 52,   2008 08 01 , http://jglobal.jst.go.jp/public/200902290212012262
Changes in Cytokine Concentrations in Serum and Whey from Dairy Cows with Peracute Mastitis Caused by Escherichia coli and Association with Clinical Findings, 久枝啓一, 有馬春樹, 園部隆久, 那須正信, 萩原克郎, 桐沢力雄, 岩井浤, 永幡肇, 日本獣医師会雑誌, 61, (6) 443 - 448,   2008 06 20 , 10.12935/jvma1951.61.443, http://jglobal.jst.go.jp/public/200902294339310284
Changes in Cytokine Concentrations in Serum and Whey from Dairy Cows with Peracute Mastitis Caused by Escherichia coli and Association with Clinical Findings, HISAEDA Keiichi, ARIMA Haruki, SONOBE Takahisa, NASU Masanobu, HAGIWARA Katsuro, KIRISAWA Rikio, IWAI Hiroshi, NAGAHATA Hajime, Journal of the Japan Veterinary Medical Association, 61, (6) 443 - 448,   2008 06 20 , http://ci.nii.ac.jp/naid/10025574175, This study was conducted to determine the concentrations of cytokines, interleukin (IL)-1β, IL-1 receptorantagonist (ra), IL-6, interferon (IFN)-γ and tumor necrosis factor (TNF)-α in serum and whey from clinicallyhealthy cows and cows with peracute coliform mastitis, and to evaluate the association of increased cytokinelevels in mastitic cows with their clinical findings. The concentrations of IL-1β, IL-6, and IFN-γ in serumand whey from E. coli mastitic cows at the onset of mastitis were apparently increased, compared with thoseof healthy cows. Increased levels of cytokine concentrations in whey were reduced during the mastitis recoveryprocess of. Correlations were found between increased levels of IL-6 in serum and whey and IFN-γ inwhey and their characteristic findings in E. coli mastitic cows at the day of diagnosis. These findings suggestthat increased levels of cytokine concentrations in dairy cows with E. coli mastitis may be associated with thepathogenesis and their characteristic findings.
An Epidemiological Survey of Hepatitis E virus in Sika Deer, Cervus nippon, in Nara Park, Japan, HAGIWARA Katsuro, TSUJI Masayoshi, KAWABUCHI Takako, TORII Haruki, KOBAYASHI Tomoko, ASAKAWA Mitsuhiko, ISHIHARA Chiaki, Japanese Journal of Zoo and Wildlife Medicine, 13, (1) 35 - 37,   2008 03 01 , http://ci.nii.ac.jp/naid/110006781658, Hepatitis E Virus(HEV) infections have been reported in deer as well as in domestic animals; however, the precise epidemiological information regarding HEV infections in the Shika Deer in Nara Park in Japan remains to be investigated. In this study, we examined the anti-HEV antibodies and HEV-RNA in sera from 173 of female sika deer in the park. The reactivity to HEV antigen in the serum samples were low levels. The detection of HEV-RNA in sera from the deer revealed no positive samples by RT-PCR analysis. In conclusion, we could not able to identify the HEV antibody and HEV-RNA positive individual in the sika deer which inhabited Nara Park. Therefore, the possibility of the HEV infection in the deer of the park would be extremely low.
An Epidemiological Survey of Hepatitis E virus in Sika Deer, Cervus nippon, in Nara Park, Japan, 萩原克郎, 辻正義, 川渕貴子, 鳥居春己, 小林朋子, 浅川満彦, 石原智明, 日本野生動物医学会誌, 13, (1) 35 - 37,   2008 03 , http://jglobal.jst.go.jp/public/200902218604749252
ブタの胆管周囲嚢胞と胆管嚢胞における病理組織学的検索, 小嶺美紗, 岡本実, 溝奥尋子, 高田豊樹, 川迫一史, 周本剛大, 萩原克郎, 谷山弘行, 北海道獣医師会雑誌, 51,   2007 08 01 , http://jglobal.jst.go.jp/public/200902288316321602
輸入牧草に混入し北海道にてその死体が確認されたホシムクドリSturnus vulgarisの記録とウイルス学的検査, 吉野智生, 国藤泰輔, 渡辺竜己, 久木田優美, 前田秋彦, 萩原克郎, 大沼学, 桑名貴, 村田浩一, 浅川満彦, 北海道獣医師会雑誌, 51, (3) 68 - 70,   2007 03 01 , http://jglobal.jst.go.jp/public/200902257435271378
腫瘍疾患を有する犬におけるリンパ球分画の動態, 山本竜太, 三輪祐一郎, 萩原克郎, 廉澤剛, 北海道獣医師会雑誌, 50,   2006 08 01 , http://jglobal.jst.go.jp/public/200902262387199566
牛群検診で高体細胞を呈する乳牛に対するステビア抽出発酵液経口投与の効果, 酒見蓉子, 青木ひとみ, 藤平ひとみ, 小岩政照, 萩原克郎, 澤向豊, 北海道獣医師会雑誌, 50,   2006 08 01 , http://jglobal.jst.go.jp/public/200902221030929126
乳房炎の新知見―ここまで進んだ乳房炎研究―サイトカインと予後診断, 萩原克郎, 獣医畜産新報, (1019) 453 - 457,   2006 06 01 , http://jglobal.jst.go.jp/public/200902261291355404
ボルナ病ウイルス感染牛群における臨床および繁殖調査成績, 三好正一, 萩原克郎, 小岩政照, 青木創, 虻川孝秀, 望月誠, 山中栄, 北海道獣医師会雑誌, 49,   2005 08 01 , http://jglobal.jst.go.jp/public/200902227189128475
敗血症小動物モデル重症化における経時的採血の影響とその対策, 須田康一, 北川雄光, 小沢壮治, 才川義朗, 上田政和, 矢野和仁, 大山隆史, 小熊潤也, 田淵悟, 安藤崇史, 宮庄拓, 萩原克郎, 横田博, 石坂彰敏, 北島政樹, 外科と代謝・栄養, 39,   2005 06 15 , http://jglobal.jst.go.jp/public/200902231199582619
Serum cytokine levels evaluated in neonatal calves in relation to disease incidence up to 4 months after colostrum ingestion, 平子毅, 久枝啓一, 泉賢一, 黒沢隆, 萩原克郎, 桐沢力雄, 岩井ひろし, 酪農学園大学紀要 自然科学編, 29, (2) 171 - 175,   2005 04 20 , http://jglobal.jst.go.jp/public/200902278175899570
Serum cytokine levels evaluated in neonatal calves in relation to disease incidence up to 4 months after colostrum ingestion, HIRAKO Tsuyoshi, HISAEDA Keiichi, IZUMI Kenichi, KUROSAWA Takashi, HAGIWARA Katsuro, KIRISAWA Rikio, IWAI Hiroshi, Journal of the College of Dairying. Natural science, 29, (2) 171 - 175,   2005 04 , http://ci.nii.ac.jp/naid/110002970372
Changes in Interleukin-6 Concentration in Peripheral Blood of Pre- and Post-Partum Dairy Cattle and Its Relationship to Postpartum Reproductive Diseases(Theriogenology), ISHIKAWA Yukikazu, NAKADA Ken, HAGIWARA Katsuro, KIRISAWA Rikio, IWAI Hiroshi, MORIYOSHI Masaharu, SAWAMUKAI Yutaka, The journal of veterinary medical science, 66, (11) 1403 - 1408,   2004 11 25 , http://ci.nii.ac.jp/naid/110003963621, Reproductive diseases after parturition are a serious problem in dairy cattle. It is important to predict postpartum reproductive diseases early and to develop prophylaxis. The objectives of this study were to demonstrate changes in the peripheral blood concentration of interleukin-6 (IL-6) before parturition, which was mainly produced by T helper 2 type (Th2) cells, and to investigate a correlation between the IL-6 concentration and the occurrence of the postpartum retained placenta, endometritis and/or follicular cyst in dairy cattle. Twenty-seven Holstein-Friesian cows were used for this study. Thirteen had no clinical disease, 8 had retained placenta, 4 were diagnosed with endometritis by vaginal inspection, and 2 were diagnosed with follicular cyst by rectal palpation at 1 and 2 months after parturition. Blood samples were collected 60 days pre- and post-partum. They used for IL-6, progesterone (P_4) and estradiol-17β (E_2) concentration determination. This study showed that the IL-6 concentration prepartum was higher than postpartum. Low levels of IL-6 and P_4 in peripheral blood prepartum tended to affect retained placenta and a high level of IL-6 prepartum tended to affect endometritis. These results indicate that measurement of change in the IL-6 concentration during pregnancy is one useful tool for predicting crisis in postpartum reproductive diseases in dairy cattle.
A Preliminary Report of Parasitological and Microbiological survey of Freeranging Japanese Macaques (Macaca fuscata (Blyth)) in Boso Peninsula, Japan, SATOYOSHI Ayako, KABAYA Hajime, HAGIWARA Ko, TANIYAMA Hiroyuki, YOSHIZAWA Kazunori, TUJI Masayoshi, HAGIWARA Kasturo, MURAMATSU Yasukazu, ASAKAWA Mitsuhiko, Japanese Journal of Zoo and Wildlife Medicine, 9, (2) 79 - 83,   2004 09 01 , http://ci.nii.ac.jp/naid/110002665451, Because alien macaques (probably, Macaca mulatta) occur in the southern part of the Boso Peninsula, Japan, there is a possibility that their parasites and/or other pathogens may infect Japanese macaques (M.fuscata). To verify the possibility, this study was conducted mainly on parasites that included protozoan, helminth and arthropod, and other pathogens including Rickettsia and the Borna virus of 50 free ranging Japanese macaques in the peninsula. Consequently, 5 arthropod and nematode parasite species common to the Japanese macaque, namely Pedicinus obtusus, P.eurygaster, Streptopharagus pigmentatus, Strongyloides fulleborni, and Trichuris trichiura, were obtained in this survey. It is the first record of sites where Pedicinus spp. are seen in a host species.Coccidia and Babesia sp. were not obtained. Coxciella and the Borna virus from the same host samples will be published elsewhere.
Epizootiologic Survey for Babesia microti among Small Wild Mammals in Northeastern Eurasia and a Geographic Deversity in the β-Tubulin Gene Sequences(Public Health), ZAMOTO Aya, TSUJI Masayoshi, WEI Qiang, CHO Shin-Hyeong, SHIN E-Hyun, KIM Tong-Soo, LEONOVA Galina N., HAGIWARA Katsuro, ASAKAWA Mitsuhiko, KARIWA Hiroaki, TAKASHIMA Ikuo, ISHIHARA Chiaki, The journal of veterinary medical science, 66, (7) 785 - 792, s-vii-s-viii,   2004 07 25 , http://ci.nii.ac.jp/naid/110003960139, We previously reported that small wild rodents in Japan harbor two types of novel Babesia microti-like parasites(designated as Hobetsu and Kobe types), but not the type commonly found in the northeastern United States(U. S. type) where human babesiosis is endemic. To determine whether these new types of parasites are distributed in places surrounding Japan, an epizootiologic survey was undertaken in three geographically distant areas in northeastern Eurasia ; South Korea, Vladivostok in Russia, and Xinjiang in China. Blood samples were collected from a total of 387 animals comprising 24 species. DNAs extracted from the samples were tested by nested PCR targeting babesial nuclear small-subunit rRNA gene(rDNA), which revealed that small rodents harboring B. microti exist in all three survey areas. Sequence analysis showed that all PCR-positive samples had rDNA sequences virtually identical to that of U. S.-type B. microti. However, when β-tubulin gene sequences were compared, evident geographic variations were seen. By use of primers specific for each of the β-tubulin genes of Kobe-, Hobetsu-, and U. S.-type parasites, a type-specific PCR was developed. Parasite with Hobetsu- or Kobe-type sequence was not detected from any of the three survey areas. These findings suggest that U. S.-type B. microti is widely distributed among small wild mammals in temperate zones of not only North America, but also Eurasia, whereas that Hobetsu- and Kobe-type parasites may be uniquely distributed in Japan.
脳に感染するウイルス―日本におけるボルナ病ウイルスについて―, 萩原克郎, 酪農ジャーナル, 56, (7) 33 - 35,   2003 07 01 , http://jglobal.jst.go.jp/public/200902213357486381
Cytokine Profile of a Holstein Calf with Bovine Leukocyte Adhesion Deficiency during the Acute-Phase Inflammatory Response(Immunology), NAGAHATA Hajime, HAGIWARA Katsuro, KASAMATSU Masahiko, HIGUCHI Hidetoshi, KUROSAWA Takashi, The journal of veterinary medical science, 64, (12) 1141 - 1143,   2002 12 25 , http://ci.nii.ac.jp/naid/110003886083, Changes in interleukin (IL)-1β, IL-6 and IL-8 in serum, and their mRNA expression on neutrophils from a 4.6-month old Holstein young calf with bovine leukocyte adhesion deficiency (BLAD) during the acute phase were evaluated. IL-1β concentrations in the serum of the calf with BLAD at age 143-162 days ranged from 8.7 to 16.6 ng/ml, whereas the values were less than 2.7 ng/ml in control calves. Serum IL-6 (0.04 ng/ml) was only detected on the 1st day when the animal was diagnosed with the BLAD. IL-1β and IL-8 mRNA expression on neutrophils from the affected calf appeared to be similar to those of controls. Serum cysokine levels and their mRNA expression on neutrophils from the calf with BLAD appeared to be little affected by the deficient expression of 132-integrin on leukocytes, and are considered to be modulated by the inflammatory stimuli.
Analysis of Serum Cytokine Profile in a Holstein Heifer with Leukocyte Adhesion Deficiency Which Survived for Long Period(Clinical Pathology), NAGAHATA Hajime, HAGIWARA Katsuro, HIGUCHI Hidetoshi, KIRISAWA Rikio, IWAI Hiroshi, The journal of veterinary medical science, 64, (8) 683 - 687, s・iv,   2002 08 25 , http://ci.nii.ac.jp/naid/110003920898, Serum cytokine levels and their expression of mRNA on neutrophils from a bone marrow (BM) transplanted heifer with leukocyte adhesion deficiency were evaluated. The clinical condition of the affected heifer was relatively stable after BM-transplantation. Persistent hyper γ-globulinemia and increased serum immunoglobulin G (IgG) concentrations were monitored longitudinally. The concentration of interleukin (IL)-1β in serum from the affected heifer ranged from 15.8 to 321.7 ng/ml, and maximum concentration occurred at the time which coincided with peak IL-6. Serum levels of IL-6 ranged from 0.32 to 27.9 ng/ml, and they appeared to be associated with the increment of serum IgG in the affected heifer. mRNAs for IL-1β, IL-6, IL-8 and granulocyte and macrophage colony stimulating factor (GM-CSF) were increased in neutrophils from the affected heifer compared to controls. Persistent hyper γ-globulinemia of the affected heifer appeared to be associated with enhanced mRNA expression for IL-6 and its serum levels. These findings suggest that humoral immunity of the affected heifer is activated and the production of neutrophils appears to be enhanced under the incapability of β_2 integrin-mediated functions of phagocytic cells.
新生子牛血清中サイトカイン動態とその意義, 山中仁木, 萩原克郎, 桐沢力雄, 岩井ひろし, 北海道獣医師会雑誌, 46,   2002 08 01 , http://jglobal.jst.go.jp/public/200902119911389516
ボルナ病発生牛のボルナ病ウイルス垂直感染, 岡本実, 小岩政照, 古岡秀文, 萩原克郎, 桐沢力雄, 岩井ひろし, 鈴木隆秀, 谷山弘行, 北海道獣医師会雑誌, 46,   2002 08 01 , http://jglobal.jst.go.jp/public/200902186707353960
Effect of Interleukin-1 Receptor Antagonist on Concanavalin A Response of Peripheral Blood Mononuclear Cells from Newborn Calves, YAMANAKA Hitoki, KATAGIRI Yoshito, DOMI Mayumi, HAGIWARA Katsuro, KIRISAWA Rikio, IWAI Hiroshi, The journal of veterinary medical science, 63, (8) 933 - 935,   2001 08 25 , http://ci.nii.ac.jp/naid/110003920721, The coexistence of interleukin (IL)-1β with IL-1 receptor antagonist (ra) in bovine colostrum and the possibility of simultaneous transfer of these cytokines to neonates via colostrum have been demonstrated. In the present study, we investigated the effect of IL-1ra on the mitogenic response of calf peripheral blood mononuclear cells (PBMC) stimulated by concanavalin A (ConA), which was mediated by IL-1. Pretreatment of PBMC with recombinant bovine (rb) IL-1ra alone significantly suppressed the proliferation of ConA-stimulated cells. However, in the presence of rbIL-1β, the suppressive activity of rbIL-1ra was counteracted. These results suggest that coexistence of IL-1ra with IL-1 in colostrum may have no effect on the activation of the neonatal immune system by IL-1β.
Interferon-γ and tumor necrosis factor-α levels in sera and whey of cattle with naturally occurring coliform mastitis(Immunology), HISAEDA K., HAGIWARA K., EGUCHI J., YAMANAKA H., KIRISAWA R., IWAI H., The journal of veterinary medical science, 63, (9) 1009 - 1011,   2001 08 25 , http://ci.nii.ac.jp/naid/110003920736, Concentrations of interferon-γ(IFN-γ)and tumor necrosis factor-α(TNF-α)were determined in serum and whey samples from cattle with naturally occurring coliform mastitis for two weeks after onset using bovine INF-γ- and TNF-α-specific ELISA. In serum and whey samples from healthy cows, IFN-γ was almost undetectable and TNF-α was detected at low levels. At the onset of illness, INF-γ in sera and whey and TNF-α in whey from the mastitic cows were significantly higher than their respective values in healthy cows. Concentrations of IFN-γ and TNF-α in whey from mastitic cattle decreased significantly as the cows recovered.
乳房炎り患時における臨床症状と炎症性サイトカインの動態, 秦秀明, 岡井和彦, 田原口貞夫, 萩原克郎, 北海道獣医師会雑誌, 45,   2001 08 01 , http://jglobal.jst.go.jp/public/200902130379634446
わが国における馬ボルナ病の初発生例, 岡本実, 萩原克郎, 桐沢力雄, 岩井ひろし, 谷山弘行, 北海道獣医師会雑誌, 45, (7) 205 - 207,   2001 07 01 , http://jglobal.jst.go.jp/public/200902197325119888
ELISA for Bovine Interleukin-1 Receptor Antagonist and Its Application to Mastitic Sera and Whey, YAMANAKA Hitoki, HISAEDA Keiichi, HAGIWARA Katsuro, KIRISAWA Rikio, IWAI Hiroshi, The journal of veterinary medical science, 62, (6) 661 - 664,   2000 06 25 , http://ci.nii.ac.jp/naid/110003920385, A sandwich ELISA for the bovine IL-1 receptor antagonist(bIL-lra)was developed using recombinant(r)bIL-lra produced by Escherichia coli, anti-rbIL-lra rabbit IgG, its biotinylated one and avidin-peroxidase.This ELISA system enabled detection of rbIL-lra at a concentration of more than 2ng / ml.This ELISA was applied to quantitation of bIL-lra in sera and whey of mastitic and healthy cows.The results indicate that although IL-lra levels in healthy and mastitic sera and whey were comparable, serum IL-lra / IL-lβ ratio of euthanized cows was significantly lower than that of the recovered.
A Method for Analysis of Cell to Cell Transmission of Theileria sergenti Piroplasms, TERADA Yutaka, TSUJI Masayoshi, NAKAMURA Yurie, HAGIWARA Katsuro, TAKAHASHI Kiyoshi, ISHIHARA Chiaki, The journal of veterinary medical science, 60, (1) 115 - 117,   1998 01 25 , http://ci.nii.ac.jp/naid/110003918388, We tried to develop an experimental system for quantifying piroplasm transmission in Theileria sergenti infection using a SCID mouse model, of which red blood cells (RBC) had been substituted with bovine (Bo). A mouse hybridoma cell producing antibody against Holstein RBC was established for distinguishing Holstein RBC from Japanese Black RBC by a immuno-staining method. T. sergenti piroplasms in RBC were stained by 4', 6-diamidino-2-phenylindole. With the aid of these techniques the piroplasm transmission from one to another race of Bo-RBCs could successfully be quantified in SCID mice.
Studies on an experimental animal model for Theileria sergenti infection, HAGIWARA Katsuro, Journal of the College of Dairying. Natural science, 20, (1) 131 - 132,   1995 10 , http://ci.nii.ac.jp/naid/110000363115
Transfusion with Xenogeneic Erythrocytes into SCID Mice and Their Clearance from the Circulation, ISHIHARA Chiaki, TSUJI Masayoshi, HAGIWARA Katsuro, HIOKI Kyoji, ARIKAWA Jiro, AZUMA Ichiro, The journal of veterinary medical science, 56, (6) 1149 - 1154,   1994 12 15 , http://ci.nii.ac.jp/naid/110003916178, We have previously demonstrated that the red blood cells (RBCs) in the blood circulation of SCID mice could be almost completely substituted with bovine RBCs by means of repeated transfusions. In the present study, transfusion experiments were carried out with RBCs from various animal species to investigate the ability of SCID mice to accept xenogeneic RBCs. Bovine (Bo), equine (Eq), human (Hu) and murine (Mu) RBCs were labeled with a fluorescent dye to trace their trafficking in the blood stream. Following the intravenous injection these RBCs were cleared from the circulation at various rates; the 1/100 reduction time in the labeled cell counts was 2 to 7 days, 4 to 7 hr and 1 to 2 hr with Bo-, Hu- and Eq-RBCs, respectively. In contrast, labeled Mu-RBCs from not only syngeneic but also allogeneic mouse strain were able to stay over 50 days. The difference in clearance rates was attributable to the difference in uptake of the RBCs by the splenic macrophages. The clearance rates of labeled RBCs were significantly decreased by co-transfusion with unlabeled RBCs of the same species, indicating that xenogeneic RBCs were recognized by the reticulo-endothelial system of SCID mice in a species-specific fashion. Furthermore, at least in the case of Hu-RBC, the complement component 3 may play a role in the Hu-RBC clearance in SCID mice since C3 deposition was observed on Hu-RBCs but not on Bo- and Eq-RBCs.
The Detection of a Mutation of CD18 Gene in Bovine Leukocyte Adhesion Deficiency (BLAD), TAJIMA Motoshi, IRIE Mitsuhiro, KIRISAWA Rikio, HAGIWARA Katsuro, KUROSAWA Takashi, TAKAHASHI Kiyoshi, The journal of veterinary medical science, 55, (1) 145 - 146,   1993 02 15 , http://ci.nii.ac.jp/naid/110003915772, Two calves (5 and 9 months old) affected with pneumonia and gingivitis were also diagnosed as having bovine leukocyte adhesion deficiency (BLAD). The gene of leukocyte adhesion molecule CD18 in these BLAD calves and their dams (carrier) were examined by means of polymerase chain reaction (PCR) and digestion of restriction endonuclease. The splicing in mRNA coded CD18 reported in human LAD was not recognized in BLAD on the basis of the results of PCR amplification. The region including the portion of point mutation, which corresponded to the region reported in the human patient, was amplified by PCR, and the PCR product was then digested with TaqI. An obvious difference was recognized in the pattern of digestion among healthy calves, BLAD calves and their dams. In BLAD, therefore, the point mutation reported in human patients was strongly suggested. Moreover it may be a method able to be used in detecting the carrier.
Inhibitory effect of heparin on red blood cell invasion by Theileria sergenti merozoites, International Journal for Parasitology, 27,   1997 , 10.1016/S0020-7519(97)00031-3
Demonstration of Borna Disease virus(BDV)in Specific Regions of the Brain from Horses Positive for Serum Antibodies to BDV but Negative for BDV RNA in the Blood and Internal Organs, Medical Microbiology Immunology, 186,   1997 , 10.1007/s004300050041
High Prevalence of Borna Disease Virus Intection in Healthy Sheep in Japan, Clinical and Diagnostic Laboratory Immunology, 4, (3) 339 - 344,   1997
Studies on an experimental system for the invasion of Theileria sergenti merozoite into erythrocyte, Veterinary Parasitology, 63,   1996 , 10.1016/0304-4017(95)00914-0
Borna disease virus RNA in peripheral blood mononuclear cells obtained from healthy dairy cattle, Medical Microbiology and Immunology, 185,   1996 , 10.1007/s004300050024
Serum from Theilenia sergenti intected cattle accelerates the clearance of bovine erythrocytes in SCID mice, Parasitology Reseach, 81,   1995
The Bo-RBC-SCID mouse model for eralvating the efficacy of anti-Theileria drugs, International Journal for Parasitology, 23,   1993
Theileria sergenti infection in the Bo-RBC-SCID mouse model, Parasitology Reseach, 79,   1993
Enzymatic amplifiation and expression of bovine interleukin-1 receptor antagonist cDNA., Veterinary Immunology and Immunopathology, 62,   1998 , 10.1016/S0165-2427(98)00099-3
A sulfated chitin inhibits hemagglutination by Theileria sergenti nerozoifes, Carbohydrate Polymers, 39, (3) 245 - 248,   1999 , 10.1016/S0144-8617(99)00016-8
High suscepfibility of Mongolian gerbil(Meriones unguiculatus)to Borna disease virus, Vaccine, 17,   1999 , 10.1016/S0264-410X(98)00222-9
Replication Ability in Vitro and in Viro of Equine Infectious Anemia Virus Avirulent Japanese Strain, Virology, 266, (1) 129 - 139,   2000 , 10.1006/viro.1999.0076
Detection of Borna disease virus in a pregnant mare and her fetus, Veterinary microbiology, 72,   2000 , 10.1016/S0378-1135(99)00206-0
ELISA for Bovine Interlevkin-1 Receptor Antagonist and Its Application to mactitic sera and whey, The Journal of Veterinary Medical Science, 62, (6) 661 - 664,   2000 , 10.1292/jvms.62.661

Research Grants & Projects

Study on bovine cytokines
Epidemiological Studies on Borna Disease Virus
Epidemiological studies on Hepatitis E Virus


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