School of Veterinary Medicine, Department of Veterinary Medicine
萩原克郎 ハギワラ カツロウ
E-Mail:
Website:

Last Update :2020/09/02

Researcher Profile and Settings

Name

HAGIWARA Katsuro

Affiliation (Master)

School of Veterinary Medicine, Department of Veterinary Medicine

Affiliation & Job

School of Veterinary Medicine, Department of Veterinary Medicine
Professor

Degree

(BLANK)

Association Memberships

THE JAPANESE ASSOCIATION FOR INFECTIOUS DISEASES

Research Activities

Research Areas

Life sciences, Veterinary medicine
Life sciences, Veterinary medicine
Life sciences, Veterinary medicine

Research Interests

Virology, Intestinal microbiota, Immunity, Tumor immunity,

Published Papers

Imaging of micro-organisms on topsoil particles collected from different landscape in the Gobi Desert, Morine Kuribayashi, Keiichi Kawano, Yuta Demura, Kenji Baba, Yuki Sofue, Purevsuren Tsedendamba, Tamaki Matsumoto, Katsuro Hagiwara, Olaf Karthaus, Kenji Kai, Buho Hoshino, EDP Sciences, EDP Sciences, 99, 01011, 1, 4, 20190600, Refereed
Socio-economic factors associated with voluntary rabies control measures in Vietnam., Hazumu Kadowaki, Phuc Pham Duc, Kazuo Sato, Pham Thi Minh Phuong, Katsuro Hagiwara, Kohei Makita, Preventive veterinary medicine, Preventive veterinary medicine, 157, 105, 114, 20180901, Refereed
Regulation of Constitutive Interferon-Stimulated Genes (Isgs) in Tumor Cells Contributes to Enhanced Antitumor Response of Newcastle Disease Virus-Infected Tumor Vaccines., Mai Takamura-Ishii, Takaaki Nakaya, Katsuro Hagiwara, Cancers, Cancers, 10, 6, 20180606, Refereed
Influence of changes in the intestinal microflora on the immune function in mice., Shigefumi Kishida, Yuko Kato-Mori, Katsuro Hagiwara, The Journal of veterinary medical science, The Journal of veterinary medical science, 80, 3, 440, 446, 20180324, Refereed
Zoonotic Enterobacterial Pathogens Detected in Wild Chimpanzees., Matthew R McLennan, Hirotake Mori, Aongart Mahittikorn, Rapeepun Prasertbun, Katsuro Hagiwara, Michael A Huffman, EcoHealth, EcoHealth, 15, 1, 143, 147, 20180300, Refereed
Bacteriophage ΦSA012 Has a Broad Host Range against Staphylococcus aureus and Effective Lytic Capacity in a Mouse Mastitis Model., Hidetomo Iwano, Yusuke Inoue, Takuji Takasago, Hironori Kobayashi, Takaaki Furusawa, Kotomi Taniguchi, Jumpei Fujiki, Hiroshi Yokota, Masaru Usui, Yasunori Tanji, Katsuro Hagiwara, Hidetoshi Higuchi, Yutaka Tamura, Biology, Biology, 7, 1, 20180109, Refereed
Detection of dry lake beds formation and estimate of environmental regime shift in semi-arid region, BABA KENJI, Journal of Arid Land Studies, Journal of Arid Land Studies, 28, 5, 109, 113, 20180000, Refereed
Problems in the Protection of Reintroduced Przewalski's Horses (Equus ferus przewalskii) Caused by Piroplasmosis., Munkhbat Tarav, Makoto Tokunaga, Tatsunari Kondo, Yuko Kato-Mori, Buho Hoshino, Usukhjargal Dorj, Katsuro Hagiwara, Journal of wildlife diseases, Journal of wildlife diseases, 53, 4, 911, 915, 20171000, Refereed
Induction of antitumor response to fibrosarcoma by Newcastle disease virus-infected tumor vaccine., Mai Takamura-Ishii, Takahiro Miura, Takaaki Nakaya, Katsuro Hagiwara, Medical oncology (Northwood, London, England), Medical oncology (Northwood, London, England), 34, 10, 171, 171, 20170901, Refereed
Postnatal changes in the relative abundance of intestinal Lactobacillus spp. in newborn calves., Tadashi Takino, Yuko Kato-Mori, Daisuke Motooka, Shota Nakamura, Tetsuya Iida, Katsuro Hagiwara, The Journal of veterinary medical science, The Journal of veterinary medical science, 79, 3, 452, 455, 20170318, Refereed
Neutralizing activities against seasonal influenza viruses in human intravenous immunoglobulin., Hiroyuki Onodera, Takeru Urayama, Kazue Hirota, Kazuhiro Maeda, Ritsuko Kubota-Koketsu, Kazuo Takahashi, Katsuro Hagiwara, Yoshinobu Okuno, Kazuyoshi Ikuta, Mikihiro Yunoki, Biologics : targets & therapy, Biologics : targets & therapy, 11, 23, 30, 20170000, Refereed
Sero-epidemiological analysis of vertical transmission relative risk of Borna disease virus infection in dairy herds., Tatsuya Ando, Tadashi Takino, Kohei Makita, Motoshi Tajima, Masateru Koiwa, Katsuro Hagiwara, The Journal of veterinary medical science, The Journal of veterinary medical science, 78, 11, 1669, 1672, 20161201, Refereed
Change in the responsiveness of interferon-stimulated genes during early pregnancy in cows with Borna virus-1 infection., Tadashi Takino, Taku Okamura, Tatsuya Ando, Katsuro Hagiwara, BMC veterinary research, BMC veterinary research, 12, 1, 253, 253, 20161114, Refereed
Phage Therapy Is Effective in a Mouse Model of Bacterial Equine Keratitis., Takaaki Furusawa, Hidetomo Iwano, Yutaro Hiyashimizu, Kazuki Matsubara, Hidetoshi Higuchi, Hajime Nagahata, Hidekazu Niwa, Yoshinari Katayama, Yuta Kinoshita, Katsuro Hagiwara, Tomohito Iwasaki, Yasunori Tanji, Hiroshi Yokota, Yutaka Tamura, Applied and environmental microbiology, Applied and environmental microbiology, 82, 17, 5332, 9, 20160901, Refereed
Hepatitis E virus derived from different sources exhibits different behaviour in virus inactivation and/or removal studies with plasma derivatives., Mikihiro Yunoki, Hiroyuki Tanaka, Kadue Takahashi, Takeru Urayama, Shinji Hattori, Shoji Ideno, Rie Furuki, Kaoru Sakai, Katsuro Hagiwara, Kazuyoshi Ikuta, Biologicals : journal of the International Association of Biological Standardization, Biologicals : journal of the International Association of Biological Standardization, 44, 5, 403, 11, 20160900, Refereed
Change in the responsiveness of interferon-stimulated genes during early pregnancy in cows with Borna virus-1 infection, Takino,T, Okamura,T, Ando,T, Hagiwara,K, J.Jpn.Vet.Med. Assoc, J.Jpn.Vet.Med. Assoc, 53, 577, 581, 20160600
Nutritional Indicators in Holstein Dairy Heifers Infected with Respiratory Syncytial Virus with Referring to Changes in Lipid Profile, Tumor Necrosis Factor-a and Acute Phase Proteins, Enas Elmeligy Arafat Khalphallah, Shin Oikawa, Masato Motokawa, Ken Nakada, Katsuro Hagiwara, Ahmad A. Aamer, Tharwat AbdelAll, 3, 1, 2, 28, 20160000, Refereed
Isolation and characterization of a novel Rhabdovirus from a wild boar (Sus scrofa) in Japan., Kouji Sakai, Katsuro Hagiwara, Tsutomu Omatsu, Chinami Hamasaki, Ryusei Kuwata, Hiroshi Shimoda, Kazuo Suzuki, Daiji Endoh, Noriyo Nagata, Makoto Nagai, Yukie Katayama, Mami Oba, Ichiro Kurane, Masayuki Saijo, Shigeru Morikawa, Tetsuya Mizutani, Ken Maeda, Veterinary microbiology, Veterinary microbiology, 179, 3-4, 197, 203, 20150930, Refereed
Viral detection by high-throughput sequencing., Daisuke Motooka, Shota Nakamura, Katsuro Hagiwara, Takaaki Nakaya, Methods in molecular biology (Clifton, N.J.), Methods in molecular biology (Clifton, N.J.), 1236, 125, 34, 20150000, Refereed
Comparative characteristics of the home ranges of domestic and wild animals in arid and sub-arid Afro-Eurasia, and watering places as hot spot for the pasture degradation, Hoshino BUHO, Hiroshi NAWATA, Kenji KAI, Hiroshi YASUDA, Kenji BABA, Sumiya GANZORIG, SURIGA, Manayeva KARINA, Tsedendamba PUREVSUREN, Miki HASHIMOTO, Kenji KAWASHIMA, Jun NODA, Katsuro HAGIWARA, Yuka SHIBATA, Journal of Arid Land Studies, Journal of Arid Land Studies, 24, 1, 51, 56, 20140600, Refereed
Temporal Changes in Environmental Health Risks and Socio-psychological Status in Areas Affected by the 2011 Tsunami in Ishinomaki, Japan, Makita, K, Inoshita, K, Kayano, T, Uenoyama, K, Hagiwara, K, Asakawa, M, Ogawa, K, Kawamura, S, Noda, J, Sera, K, Sasaki, H, Nakatani, N, Higuchi, H, Ishikawa, N, Iwano, H, Tamura, Y, Environment and Pollution, Environment and Pollution, 3, 1, 20130000, Refereed
The influence of Borna disease viral infection on dairy cow reproduction., Katsuro Hagiwara, Tatsuya Ando, Masateru Koiwa, The Journal of veterinary medical science, The Journal of veterinary medical science, 74, 4, 419, 21, 20120400, Refereed
Interleukin-6 in quarter milk as a further prediction marker for bovine subclinical mastitis., Yoko Sakemi, Yutaka Tamura, Katsuro Hagiwara, The Journal of dairy research, The Journal of dairy research, 78, 1, 118, 21, 20110200, Refereed
Fermentation metabolites from Lactobacillus gasseri and Propionibacterium freudenreichii exert bacteriocidal effects in mice., Yuko Kato-Mori, Takenori Orihashi, Yuta Kanai, Michiko Sato, Kenji Sera, Katsuro Hagiwara, Journal of medicinal food, Journal of medicinal food, 13, 6, 1460, 7, 20101200, Refereed
Significant neutralizing activity of human immunoglobulin preparations against pandemic 2009 H1N1., Mikihiro Yunoki, Ritsuko Kubota-Koketsu, Takeru Urayama, Tadahiro Sasaki, Du Analiwa, Yuko Konoshima, Shoji Ideno, Yuki Fukunaga, Saeko Morikawa, Satoshi Hiroi, Kazuo Takahashi, Yoshinobu Okuno, Katsuro Hagiwara, Kazuyoshi Ikuta, British journal of haematology, British journal of haematology, 148, 6, 953, 5, 20100300, Refereed
Leptin inhibits mitogen-induced proliferation of peripheral T lymphocytes from Holstein cows., Mohamed Ahmed, Kazuhiro Kimura, Mohamed Soliman, Daisuke Yamaji, Yuko Okamatsu-Ogura, Katsumi Ishioka, Kennedy Makondo, Katsuro Hagiwara, Masayuki Saito, Veterinary journal (London, England : 1997), Veterinary journal (London, England : 1997), 176, 3, 361, 8, 20080600, Refereed
Characterization of a Borna disease virus field isolate which shows efficient viral propagation and transmissibility., Yohei Watanabe, Madiha S Ibrahim, Katsuro Hagiwara, Minoru Okamoto, Wataru Kamitani, Hideyuki Yanai, Naohiro Ohtaki, Yohei Hayashi, Hiroyuki Taniyama, Kazuyoshi Ikuta, Keizo Tomonaga, Microbes and infection, Microbes and infection, 9, 4, 417, 27, 20070400, Refereed
Distribution of PrP(Sc) in cattle with bovine spongiform encephalopathy slaughtered at abattoirs in Japan., Iwata N, Sato Y, Higuchi Y, Nohtomi K, Nagata N, Hasegawa H, Tobiume M, Nakamura Y, Hagiwara K, Furuoka H, Horiuchi M, Yamakawa Y, Sata T, Japanese journal of infectious diseases, Japanese journal of infectious diseases, 59, 2, 100, 107, 20060400, Refereed
Molecular characterization and functional expression of equine interleukin-1 type I and type II receptor cDNAs., Rikio Kirisawa, Norikazu Hashimoto, Mizuho Tazaki, Hitoki Yamanaka, Risako Ishii, Katsuro Hagiwara, Hiroshi Iwai, Veterinary immunology and immunopathology, Veterinary immunology and immunopathology, 109, 3-4, 219, 31, 20060215, Refereed
The role of IFN-gamma in cattle infected with Theirelia sergenti., Katsuro Hagiwara, Masafumi Tokuda, Tomohisa Baba, Hitoki Yamanaka, Rikio Kirisawa, Masayoshi Tsuji, Chiaki Ishihara, Hiroshi Iwai, Veterinary parasitology, Veterinary parasitology, 127, 2, 105, 10, 20050120, Refereed
Changes in interleukin-6 concentration in peripheral blood of pre- and post-partum dairy cattle and its relationship to postpartum reproductive diseases., Yukikazu Ishikawa, Ken Nakada, Katsuro Hagiwara, Rikio Kirisawa, Hiroshi Iwai, Masaharu Moriyoshi, Yutaka Sawamukai, The Journal of veterinary medical science, The Journal of veterinary medical science, 66, 11, 1403, 8, 20041100, Refereed
Epizootiologic survey for Babesia microti among small wild mammals in northeastern Eurasia and a geographic diversity in the beta-tubulin gene sequences., Aya Zamoto, Masayoshi Tsuji, Qiang Wei, Shin-Hyeong Cho, E-Hyun Shin, Tong-Soo Kim, Galina N Leonova, Katsuro Hagiwara, Mitsuhiko Asakawa, Hiroaki Kariwa, Ikuo Takashima, Chiaki Ishihara, The Journal of veterinary medical science, The Journal of veterinary medical science, 66, 7, 785, 92, 20040700, Refereed
Transient detection of proinflammatory cytokines in sera of colostrum-fed newborn calves., Hitoki Yamanaka, Katsuro Hagiwara, Rikio Kirisawa, Hiroshi Iwai, The Journal of veterinary medical science, The Journal of veterinary medical science, 65, 7, 813, 6, 20030700, Refereed
Proinflammatory cytokines in bovine colostrum potentiate the mitogenic response of peripheral blood mononuclear cells from newborn calves through IL-2 and CD25 expression., Hitoki Yamanaka, Katsuro Hagiwara, Rikio Kirisawa, Hiroshi Iwai, Microbiology and immunology, Microbiology and immunology, 47, 6, 461, 8, 20030000, Refereed
Cytokine profile of a Holstein calf with bovine leukocyte adhesion deficiency during the acute-phase inflammatory response., Hajime Nagahata, Katsuro Hagiwara, Masahiko Kasamatsu, Hidetoshi Higuchi, Takashi Kurosawa, The Journal of veterinary medical science, The Journal of veterinary medical science, 64, 12, 1141, 3, 20021200, Refereed
Analysis of serum cytokine profile in a holstein heifer with leukocyte adhesion deficiency which survived for long period., Hajime Nagahata, Katsuro Hagiwara, Hidetoshi Higuchi, Rikio Kirisawa, Hiroshi Iwai, The Journal of veterinary medical science, The Journal of veterinary medical science, 64, 8, 683, 7, 20020800, Refereed
Detection of Theileria sergenti schizonts in bovine lymph node., Hagiwara K, Takahashi K, Taniyama H, Kawamoto S, Kurosawa T, Ikuta K, Ishihara C, Int J Parasitol., Int J Parasitol., 27, 11, 1375, 1378, 19971100, Refereed
Sporogony of Theileria sergenti in the salivary glands of the tick vector Haemaphysalis longicornis., Takahashi K, Kawai S, Yaehata K, Kawamoto S, Hagiwara K, Kurosawa T, Tajima M, Sonoda M, Parasitol Res., Parasitol Res., 79, 1, 19930100, Refereed
Northeast Asian Dust Transport: A Case Study of a Dust Storm Event from 28 March to 2 April 2012., HAGIWARA Katsuro, Atmosphere, Atmosphere, 10, 69, Refereed

Books etc

Veterinary Microbiology, HAGIWARA Katsuro, Contributor, Hepatitis E virus, Buneido,   2018 06

Conference Activities & Talks

Foodborne Risks Caused by Hepatitis E Virus Distribution in Pigs in Vietna, Katsuro Hagiwara, Yukari Kezuka, Vuong Bui Nghia, Hu Suk Lee, Hung Nguyen-Viet, Kohei Makita, 67th Japan Virology Meeting,   2019 10 29
Prevention of zoonoses requires the one health concept, Katsuro Hagiwara, 17th National Virology Conference, Mongolia.,   2019 10 04 , 招待有り
Effect of persistent infection with BoDV on reproduction, Katsuro Hagiwara, 162th JVMS meeting,   2019 09 10
One Health and Hepatitis E, HAGIWARA Katsuro, Mongolia IVM research seminar,   2019 01 31
Diversity of Intestinal Microflora in Takhi, HAGIWARA Katsuro, HNP annual meeting,   2019 01 27
We live with microorganisms, HAGIWARA Katsuro, ILRI Vietnam research seminar,   2019 01 23
One health and human health, HAGIWARA Katsuro, One Health symposium講演,   2018 11 12 , 招待有り
A potential reservoir animal of hepatitis E virus in Mongolia, HAGIWARA Katsuro, 66th Japan virology meeting,   2018 10 29
Influence of ruxolitinib, Janus-kinase (JAK) inhibitor, on Murine leukemia Virus (MuLV)multiplication, HAGIWARA Katsuro, 66th Japan virology meeting,   2018 10 29
Possibility of Mx expression to reflect differences in Influenza virus susceptibility in birds, HAGIWARA Katsuro, 66th Japan virology meeting,   2018 10 29
The concept of Onea Health in Zoonosis, HAGIWARA Katsuro, Mahidol University Trop.Med. Seminar,   2018 08 27 , 招待有り
reatment of JAK inhibitor improve the infectivity to tumor and immune response by Newcastle disease virus-infected tumor vaccine, ○Mai Ishii, Takaaki Nakaya, Katsuro Hagiwara,   2017 10
Anti-viral effect of Lactobacilli on Influenza A (H1N1) virus infection., Yuko Kato-Mori, Shigefumi Kishida, Minoru Okamoto, Katsuro Hagiwara, International Union of Microbiological Societies Congresses,   2017 06

Misc

Silage production using Lactobacillus plantarum which separated from the natural world, 石井智美, 森ゆうこ, 萩原克郎, 酪農学園大学紀要 自然科学編(Web), 41,   2016 10 , http://jglobal.jst.go.jp/public/201602217359111392
呼吸の病態生理学 第51回 呼吸器疾患の臨床微生物学 2:呼吸器疾患関連ウイルスによる発症機序(後編), 萩原克郎, 鈴木一由, 加藤敏英, 臨床獣医, 34,   2016 10 01 , http://jglobal.jst.go.jp/public/201602292192288446
道内で特用家畜・家禽として飼育されるアルパカ(Vicugna pacos)とエミュー(Dromaius novaehollandiae)の寄生虫保有状況に関する予備試験, 高野結衣, 竹内萌香, 立本完吾, 萩原克郎, 浅川満彦, 北海道獣医師会雑誌, 60,   2016 09 10 , http://jglobal.jst.go.jp/public/201602257790438430
呼吸の病態生理学 第50回 呼吸器疾患の臨床微生物学 1:呼吸器疾患関連ウイルスによる発症機序(前編), 萩原克郎, 鈴木一由, 加藤敏英, 臨床獣医, 34,   2016 09 01 , http://jglobal.jst.go.jp/public/201602214653251230
エゾシカにおけるE型肝炎ウイルス感染の疫学調査, 萩原克郎, 高橋万世, 加藤(森)ゆうこ, 加藤(森)ゆうこ, 岡本実, 浅川満彦, 日本獣医学会学術集会講演要旨集, 159th,   2016 08 30 , http://jglobal.jst.go.jp/public/201602216726879762
採卵鶏3種におけるワクチン応答の違いについて, 中村優臣, 瀧野祥司, 瀧野祥司, 峯苫健太, 加藤(森)ゆうこ, 萩原克郎, 北海道獣医師会雑誌, 60,   2016 08 10 , http://jglobal.jst.go.jp/public/201602269636157263
大気ブラックカーボン及びその役割を知る, 安成哲平, 兼保直樹, 能田淳, 星野仏方, 野口泉, 村尾直人, 秋山雅行, 藤岡真路, 馬場賢治, 萩原克郎, 甲斐憲次, 山口高志, 鈴木啓明, 松本利恵, 細氷(Web),   2016 , http://jglobal.jst.go.jp/public/201802267117231037
北海道内乳牛におけるボルナ病ウイルスの感染状況の把握, 小林洋平, 近藤達成, 加藤(森)ゆうこ, 安藤達哉, 小岩政照, 萩原克郎, 北海道獣医師会雑誌, 59,   2015 08 10 , http://jglobal.jst.go.jp/public/201502211864098666
乳牛におけるボルナ病ウイルス母子感染の分析疫学的相対リスク, 安藤達哉, 小林洋平, 近藤達成, 蒔田浩平, 田島誉士, 萩原克郎, 小岩政照, 北海道獣医師会雑誌, 59,   2015 08 10 , http://jglobal.jst.go.jp/public/201502206183800148
全国の日本シカにおけるBabesia divergens感染状況調査, 新倉 綾, 座本, 今岡 浩一, 森川 茂, 萩原 克郎, 加藤 ゆうこ, 森, 平田 晴之, 石原 智明, 花木 賢一, 日本獣医学会学術集会講演要旨集, 158回,   2015 08
競走馬の細菌性角膜炎に対するファージセラピーの有効性, 古澤貴章, 岩野英知, 樋口豪紀, 萩原克郎, 丹羽秀和, 田村豊, 感染症学雑誌, 89,   2015 03 20 , http://jglobal.jst.go.jp/public/201502212881115120
ウイルス等感染性因子安全性評価に関する研究―異常型プリオンのin vivo検出系の評価に関する研究―, 萩原克郎, 加藤(森)ゆうこ, 岡本実, 柚木幹弘, 坂井薫, 上平崇, ウイルス等感染性因子安全性評価に関する研究 平成26年度 総括・分担研究報告書,   2015 , http://jglobal.jst.go.jp/public/201502216175824911
変異型クロイツフェルトヤコブ病(vCJD)持続発現細胞プリオン蛋白のマウスへの伝達性, 加藤(森)ゆうこ, 上平崇, 坂井薫, 柚木幹弘, 岡本実, 萩原克郎, 日本ウイルス学会学術集会プログラム・抄録集, 62nd,   2014 10 31 , http://jglobal.jst.go.jp/public/201402264149968937
牛初乳中の細菌叢と利用方法の検討, 高橋沙季, 武井誠子, 石井智美, 萩原克郎, 栄養学雑誌, 72,   2014 08 20 , http://jglobal.jst.go.jp/public/201402236455417002
生乳(初乳)中にLactobacillus plantarumを入れて発酵した際の細菌叢の変化, 武井誠子, 高橋沙季, 石井智美, 萩原克郎, 栄養学雑誌, 72,   2014 08 20 , http://jglobal.jst.go.jp/public/201402255704783221
競走馬の細菌性角膜炎に対するファージセラピーの検討, 岩野英知, 冷清水雄太郎, 古澤貴章, 樋口豪紀, 永幡肇, 萩原克郎, 丹羽秀和, 片山芳也, 木下優太, 横田博, 田村豊, 北海道獣医師会雑誌, 58,   2014 08 10 , http://jglobal.jst.go.jp/public/201402288695854604
マウス馴化vCJD株を用いた血漿分画製剤工程のプリオン除去, 上平崇, 久保純, 大久保祐士, 坂井薫, 加藤(森)ゆうこ, 萩原克郎, 生田和良, 柚木幹弘, 血液事業, 37,   2014 08 01 , http://jglobal.jst.go.jp/public/201402204544946419
大脳萎縮が顕著に認められたボルナ病ウイルス抗体陽性の猫の1例, 坂大智洋, 新野猛, 萩原克郎, 二瓶和美, チェンバーズ ジェームズ, 内田和幸, 獣医神経病学会, 40th,   2014 07 05 , http://jglobal.jst.go.jp/public/201502265315208532
プロバイオティクスとプレバイオティクス~プレバイオティクスとしての発酵代謝産物の利用~, 加藤(森)ゆうこ, 安井勝巳, 後藤正光, 萩原克郎, 宮城県獣医師会会報, 67,   2014 04 , http://jglobal.jst.go.jp/public/201702222511586476
Lactobacillus plantarum投与マウスにおける抗炎症効果の検討, 加藤(森)ゆうこ, 萩原克郎, 日本細菌学雑誌, 69,   2014 02 25 , http://jglobal.jst.go.jp/public/201402299813946154
北海道日高地方におけるエゾシカ(Cervus nippon yesoensis)の内部寄生虫相および道内エゾシカ寄生日本産肝蛭(Fasciola sp.)の分布域について, 森昇子, 三觜慶, 鈴木瑞穂, 萩原克郎, 浅川満彦, 北海道獣医師会雑誌, 58, (2) 44 - 47,   2014 02 10 , http://jglobal.jst.go.jp/public/201402244380515022
由来の異なるE型肝炎ウイルスの熱感受性の違いについて, 高橋一惠, 大久保祐士, 古木理恵, 服部眞次, 浦山健, 坂井薫, 柚木幹弘, 萩原克郎, 生田和良, 血液事業, 36, (3) 679 - 685,   2013 11 01 , http://jglobal.jst.go.jp/public/201402267579352769
血液製剤の安全対策―プリオン除去の現状―, 加藤(森)ゆうこ, 柚木幹弘, 生田和良, 萩原克郎, モダンメディア, 59, (9) 231 - 237,   2013 09 , http://jglobal.jst.go.jp/public/201302219531888142
高淘汰更新牛群におけるボルナ病ウイルスの感染状況と抗体陽性牛の母系血統的考察, 安藤達哉, 小岩政照, 萩原克郎, 及川伸, 太田浩運, 篠田浩二郎, 山本康朗, 矢崎美恵, 染谷勇介, 大野和吉, 藪崎尚弘, 阿部竜大, 北海道獣医師会雑誌, 57,   2013 08 10 , http://jglobal.jst.go.jp/public/201302275730263728
野生イノシシから分離された新規ラブドウイルスの系統解析, 大松 勉, 酒井 宏治, 前田 健, 片山 幸枝, 萩原 克郎, 下田 宙, 鈴木 和夫, 遠藤 大二, 永田 典代, 佐々 悠木子, 長井 誠, 古谷 哲也, 森川 茂, 水谷 哲也, 日本獣医学会学術集会講演要旨集, 156回,   2013 08
知床半島を中心に生息するエゾシカCervus nippon yesoensisから見出された外部寄生虫性昆虫2種の記録, 水主川剛賢, 石名坂豪, 増田泰, 小川人士, 高須恵美, 森昇子, 萩原克郎, 浅川満彦, 知床博物館研究報告, (35) 11 - 14,   2013 05 21 , http://jglobal.jst.go.jp/public/201402278195919658
Case Reports on Two Species of Parasitic Insects Obtained from Sika Deer Cervus nippon yesoensis in Shiretoko Peninsula and Its Proximity, Hokkaido, Japan, 水主川 剛賢, 石名坂 豪, 増田 泰, 小川 人士, 高須 恵美, 森 昇子, 萩原 克郎, 浅川 満彦, 知床博物館研究報告, (35) 11 - 14,   2013 05 , http://ci.nii.ac.jp/naid/120005761888, Case reports on two species of parasitic insects including Solenopotes sp. cf. binipilosus (Linognathidae: Anoplura) and Lipoptera fortisetosa (Hippoboscidae: Diptera) obtained from sika deer Cervus nippon yesoensis in Shiretoko Peninsula and its proximity, Hokkaido, Japan, were presented. Parasitism of Solenopotes sp. cf. binipilosus on C. nippon was first established, although a record of Solenopotes sp. without any morphological data was presented in the other part of Hokkaido in the 1990's. Photographs of both insect species and their brief epidemiological comments are given.
宮城県石巻市津波被災地域における環境リスクおよび仮設住宅居住者の現状, 蒔田浩平, 伊下一人, 渡辺洋子, 茅野大志, 萩原克郎, 浅川満彦, 小川健太, 能田淳, 佐々木均, 中谷暢丈, 樋口豪紀, 岩野英知, 田村豊, Journal of Epidemiology, 23,   2013 01 24 , http://jglobal.jst.go.jp/public/201502253576358126
機能性飼料投与による新生子牛の免疫機能変化, 和田雄治, 岡村卓, 加藤(森)ゆうこ, 萩原克郎, 北海道獣医師会雑誌, 56,   2012 08 10 , http://jglobal.jst.go.jp/public/201202288151910107
E型肝炎ウイルス(HEV)の熱感受性の違いについて, 高橋一恵, 大久保祐士, 古木理恵, 服部眞次, 浦山健, 坂井薫, 柚木幹弘, 萩原克郎, 生田和良, 血液事業, 35,   2012 08 01 , http://jglobal.jst.go.jp/public/201202298709979937
HEV mini‐pool‐NAT法確立とベネシスにおける原料血漿の試験成績, 皆木隆男, 井手野祥次, 上田千晶, 高橋一恵, 浦山健, 服部眞次, 坂井薫, 柚木幹弘, 萩原克郎, 生田和良, 血液事業, 35,   2012 08 01 , http://jglobal.jst.go.jp/public/201202207553492670
宮城県石巻市津波被災地域における環境リスクの評価, 蒔田浩平, 伊下一人, 茅野大志, 萩原克郎, 浅川満彦, 小川健太, 能田淳, 佐々木均, 中谷暢丈, 樋口豪紀, 岩野英知, 田村豊, 獣医疫学雑誌, 16,   2012 07 20 , 10.2743/jve.16.9, http://jglobal.jst.go.jp/public/201202245200355113
A Case of Preliminary Utilization of Commercial ELISA Kit for Detecting Excretion of Urinary 8-hydroxyguanosine (8-OHdG) in Captive Chimpanzees, Pan troglodytes, 大島由子, 水尾愛, 洲鎌圭子, 伊谷原一, 志村良治, 上林亜紀子, 高橋悟, 大沼学, 翁長武紀, 萩原克郎, 浅川満彦, 動物園水族館雑誌, 52, (4) 140 - 144,   2012 04 , http://id.ndl.go.jp/bib/023847591
Characteristics and prognosis of dairy cows with naturally occurring peracute Escherichia coli and Klebsiella pneumoniae mastitis, 久枝 啓一, 今村 智子, 園部 隆久, 杉山 美恵子, 那須 正信, 永幡 肇, 家畜衛生学雑誌, 37, (4) 121 - 130,   2012 03 , http://ci.nii.ac.jp/naid/120005761798, This study was conducted to evaluate the characteristics and prognosis of dairy cows with naturally occurring peracute Escherichia coli (E. coli) and Klebsiella pneumoniae (K. Pneumoniae) mastitis on dairy farms. Clinical findings, concentrations of acute-phase proteins and cytokines in serum and milk from dairy cows with naturally occurring coliform mastitis were evaluated. The classification values for K. pneumoniae peracute mastitis of dairy cows were respiratory rates of 40 beats/min, blood urea nitrogen (BUN) 19 mg/di, haptoglobin (Hp) in serum and milk of 2000 μg/ml and score >2. The values for total score 8, BUN17mg/dl, serum Hp2000 μg/ml and milk Hp1000 μg/ml and score for prognosis > 2 were considered to be fatal by E. coli peracute mastitis. The values for total score 8, BUN22mg/dl, serum Hp2030 μg/ml, and milk Hp2050 μg/ml and prognosis score >3 were considered to be fatal in K. pneumoniae mastitis. The evaluation of dairy cows with peracute coliform-mastitis by scoring selective measures employed may be valuable for estimating pathogen causing coliform mastitis and its prognosis.
札幌市内のペットショップで販売されていたトカゲ類の寄生虫保有状況, 岩尾一, 篠田理恵, 吉田宗則, 吉田宗則, 萩原克郎, 浅川満彦, 北海道獣医師会雑誌, 56, (1) 5 - 7,   2012 01 10 , http://jglobal.jst.go.jp/public/201202216567278425
An experiment to measure the bowel movement and lipid metabolism of female students who drank 200ml Trial Fermented Milk (TFM) for two trial periods each lasting 21 days., 石井智美, 堀江雅美, 川見祥代, 森ゆうこ, 萩原克郎, 酪農学園大学紀要 自然科学編(Web), 36,   2011 10 , http://jglobal.jst.go.jp/public/201202290807422664
An experiment to measure the bowel movement and lipid metabolism of female students who drank 200 ml Trial Fermented Milk (TFM) for two trial periods each lastins 21 days., ISHII Satomi, HORIE Masami, KAWAMI Sathiyo, MORI Yuuko, HAGIWARA Katuro, Journal of the College of Dairying. Natural science, 36, (1) 67 - 73,   2011 10 , http://ci.nii.ac.jp/naid/110008678193
野鳥で観察された高病原性鳥インフルエンザの臨床所見および簡易検査における注意点, 渡辺有希子, 亀ケ谷千尋, 齊藤慶輔, 萩原克郎, 北海道獣医師会雑誌, 55,   2011 08 10 , http://jglobal.jst.go.jp/public/201102295840725766
出荷齢豚におけるE型肝炎ウイルスの体内分布, 川見 祥代, 柚木 幹弘, 山口 照英, 生田 和良, 萩原 克郎, 日本獣医学会学術集会講演要旨集, 152回,   2011 08
マウス馴化型vCJDを用いた工程評価系;作用機序が異なる3種類のろ過膜のプリオン除去能評価, 久保純, 大久保祐士, 中川典子, 川見祥代, 坂井薫, 柚木幹弘, 萩原克郎, 生田和良, 血液事業, 34,   2011 08 01 , http://jglobal.jst.go.jp/public/201102285493397200
バイオ医薬品におけるプリオンの問題―ヒト赤血球を原料とする人工酸素運搬体をめぐる問題―, 柚木幹弘, 萩原克郎, 生田和良, 人工血液, 18, (4) 142 - 150,   2011 03 30 , http://jglobal.jst.go.jp/public/201102263905628110
E型肝炎ウイルスの核酸増幅検査(NAT)評価用標準パネルの樹立, 内田 恵理子, 岡田 義昭, 水澤 左衛子, 柚木 幹弘, 辻川 宗男, 皆木 隆男, 稲田 耕一, 小西 久郎, 五十嵐 正志, 鈴木 光, 嘉悦 洋, 下瀬 克郎, 萩原 克郎, 安江 博, 生田 和良, 鈴木 和博, 山口 照英, 日本薬学会年会要旨集, 131年会, (3) 216 - 216,   2011 03
知っておきたい!ボルナ病 ボルナ病ウイルスの疫学―家畜から野生動物まで―, 萩原克郎, 臨床獣医, 29,   2011 02 01 , http://jglobal.jst.go.jp/public/201102248323283348
E型肝炎ウイルスRNAの核酸増幅検査(NAT)用参照パネルの作製, 内田恵理子, 岡田義昭, 水澤左衛子, 柚木幹弘, 辻川宗男, 皆木隆男, 稲田耕一, 小西久郎, 五十嵐正志, 鈴木光, 嘉悦洋, 下瀬克郎, 萩原克郎, 安江博, 生田和良, 鈴木和博, 山口照英, 生化学,   2011 , http://jglobal.jst.go.jp/public/201202283172617203
Influenza A/H1N1 virus(seasonal/pandemic)に対する人免疫グロブリン製剤の抗体価, 井手野祥次, 浦山健, 辻川宗男, 柚木幹弘, 萩原克郎, 高橋和郎, 奥野良信, 生田和良, 感染症学雑誌, 84, (6) 813 - 814,   2010 11 20 , http://jglobal.jst.go.jp/public/201002219745619082
A型インフルエンザ感染マウスモデルにおける人免疫グロブリン製剤の投与効果, 小林不二夫, 萩原克郎, 浦山健, 柏原純一, 柚木幹弘, 高橋和郎, 奥野良信, 生田和良, 感染症学雑誌, 84,   2010 11 20 , http://jglobal.jst.go.jp/public/201002297550708006
動物の感染症から学ぶ Vol.5 E型肝炎ウイルス―食品媒介性の人獣共通感染症, 萩原克郎, 医学のあゆみ, 234, (12) 1131 - 1136,   2010 09 18 , http://jglobal.jst.go.jp/public/201002258908614321
Hepatitis E virus, 萩原 克郎, 医学のあゆみ, 234, (12) 1131 - 1136,   2010 09 18 , http://ci.nii.ac.jp/naid/40017273874
PEIビーズを用いたE型肝炎ウイルス(HEV)の高感度検出法の検討, 川見 祥代, 辻川 宗男, 柚木 幹弘, 山口 照英, 生田 和良, 萩原 克郎, 日本獣医学会学術集会講演要旨集, 150回,   2010 09
Relationship between hypocalcemia and cytokines in peracute mastitis of dairy cattle caused by coliform bacteria, 久枝 啓一, 今村 智子, 有馬 春樹, Journal of livestock medicine, 57, (6) 371 - 376,   2010 06 , http://ci.nii.ac.jp/naid/40017170476
反芻家畜における血液代替物としての初乳, 萩原克郎, 人工血液, 18, (1) 9 - 14,   2010 05 20 , http://jglobal.jst.go.jp/public/201002217712122430
Lactobacillus gasseriおよびPropionibacterium freudenreichiiの発酵代謝産物による自然免疫賦活化作用, 加藤(森)ゆうこ, 折橋毅典, 世良健司, 萩原克郎, 腸内細菌学雑誌, 24,   2010 04 , http://jglobal.jst.go.jp/public/201002255757754240
Infectious prion protein in the filtrate even after 15 nm filtration., Mikihiro Yunoki, Hiroyuki Tanaka, Takeru Urayama, Yuta Kanai, Aya Nishida, Maki Yoshikawa, Yuji Ohkubo, Yoshiyasu Kawabata, Katsuro Hagiwara, Kazuyoshi Ikuta, Biologicals : journal of the International Association of Biological Standardization, 38, (2) 311 - 3,   2010 03 , Refereed, 10.1016/j.biologicals.2009.10.018, The evaluation of the removal efficacy during manufacturing is important for the risk assessment of plasma products with respect to possible contamination by infectious prions, as recently reported in several papers on the potential for prion transmission through plasma products. Here, we evaluated a virus removal filter which has 15 nm pores. An antithrombin sample immediately prior to nano-filtration was spiked with prion material prepared in two different ways. The removal (log reduction factor) of prion infectivity using animal bioassays was >or=4.72 and 4.00 in two independent filtrations. However, infectivity was detected in both the pellet and supernatant following ultracentrifugation of the 15 nm filtered samples, indicating difficulty in complete removal. The data supports the conclusion that a certain amount of infectious prion protein is present as a smaller and/or soluble form (less than approximately 15 nm in diameter).
遺伝子組換え医薬品等のプリオン安全性確保のための検出手法の標準化及びプリオン除去工程評価への適用に関する研究 異常型プリオンの検出及び工程評価に関する試験研究, 生田和良, 萩原克郎, 作道章一, 柚木幹弘, 遺伝子組換え医薬品等のプリオン安全性確保のための検出手法の標準化及びプリオン除去工程評価への適用に関する研究 平成19-21年度 総合研究報告書,   2010 , http://jglobal.jst.go.jp/public/201002248869030877
遺伝子組換え医薬品等のプリオン安全性確保のための検出手法の標準化及びプリオン除去工程評価への適用に関する研究 異常型プリオンの検出及び工程評価に関する試験研究, 生田和良, 萩原克郎, 作道章一, 柚木幹弘, 遺伝子組換え医薬品等のプリオン安全性確保のための検出手法の標準化及びプリオン除去工程評価への適用に関する研究 平成21年度 総合・分担研究報告書,   2010 , http://jglobal.jst.go.jp/public/201002257776128787
Long-term shedding of hepatitis E virus in the feces of pigs infected naturally, born to sows with and without maternal antibodies., Yuta Kanai, Muneo Tsujikawa, Mikihiro Yunoki, Shoko Nishiyama, Kazuyoshi Ikuta, Katsuro Hagiwara, Journal of medical virology, 82, (1) 69 - 76,   2010 01 , Refereed, 10.1002/jmv.21647, Pigs are presumed reservoirs for hepatitis E virus (HEV) transmission to humans. To examine infection kinetics, two litters of domestic pigs (A and B, each containing 10 piglets) infected naturally with HEV were studied until pigs were 6 months old. Maternal IgG and IgA antibodies were detected in litter A piglets, but not in litter B ones. All pigs shed HEV in feces when they were 30-110 days old, and 17 developed viremia at 40-100 days of age. Phylogenetic analysis revealed a highly close sequence of HEV genotype 3 in all pigs. The serum levels of specific IgG and IgA were similar in all pigs, although IgA was not detected in the feces. Interestingly, the onset of both viremia and seroconversion was delayed significantly in litter A pigs. The kinetics of fecal virus shedding was similar in both litters; shedding was not detected after the pigs were 120 days old. The differences in the infection kinetics between litters A and B suggested that maternal antibodies delayed the onset of viremia and seroconversion. Quantitative real-time reverse transcriptase-polymerase chain reaction revealed that HEV RNA in feces peaked 10 days after initial shedding of approximately 10(6.0) copies/g. The viral load was much lower in the serum than in the feces. At 200 days of age, HEV RNA was found in the internal organs of 3 out of 13 pigs. These study findings improve the understanding of the dynamics of natural HEV transmission in pigs, which could help in controlling virus transmission from pigs to humans.
Classical Swine Influenza A(H1N1)virus及びInfluenza A(H1N1)pdm virusに対する静注用グロブリン製剤の抗体価, 井手野祥次, 纐纈律子, ドウアナリワ, 佐々木正大, 浦山健, 柚木幹弘, 萩原克郎, 高橋和郎, 奥野良信, 生田和良, 日本ウイルス学会学術集会プログラム・抄録集, 57th,   2009 10 01 , http://jglobal.jst.go.jp/public/200902270158010720
獣医医療における血液代替物の意義, 萩原克郎, 人工血液, 17,   2009 09 16 , http://jglobal.jst.go.jp/public/200902288071463678
Borna disease virus in Raccoons (Procyon lotor) in Japan., Katsuro Hagiwara, Youhei Matoba, Mitsuhiko Asakawa, The Journal of veterinary medical science, 71, (8) 1009 - 15,   2009 08 , Refereed, 10.1292/jvms.71.1009, We have examined the seroprevalence of BDV in wild Raccoons (Procyon lotor) in Hokkaido, Japan. Serum samples from raccoons were examined using ELISA and Western blot assays to detect the presence of serum antibodies that react specifically to BDV antigens. Among 549 investigated individuals, eleven (2.0%) showed a positive reaction to BDV antigens. Brain tissue samples from five individuals were subjected to RT-PCR, which detected BDV sequences in three of them. Sequence analysis revealed a high degree of genetic conservation between BDV sequences derived from raccoons and previously published sequences derived from other animal species.
黒毛和種牛および半血種馬にみられたボルナ病抗体陽性の2症例, 福永成己, 小松勝一, 大場光洋, 大西光徳, 渡邊敬文, 小岩正照, 萩原克郎, 北海道獣医師会雑誌, 53,   2009 08 01 , http://jglobal.jst.go.jp/public/200902271953078037
Lactobacillus gasseriおよびPropionibacterium freudenreichiiの発酵代謝産物混合剤がマウスにおけるリステリア菌排菌能に及ぼす影響, 加藤(森)ゆうこ, 折橋毅典, 佐藤倫子, 金井祐太, 世良健司, 萩原克郎, 腸内細菌学雑誌, 23,   2009 04 , http://jglobal.jst.go.jp/public/200902286153904114
Preliminary Research on the Excretion of Urinary 8-Hydroxyguanosine (8-OHdG) as a Marker of Protozoan Parasites Infection in Captive Western Lowland Gorillas (Gorilla gorilla gorilla), MIZUO Ai, ONUMA Manabu, ONAGA Takenori, HAGIWARA Katsuro, SANADA Yoshinori, ASAKAWA Mitsuhiko, OHSHIMA Yuko, IMANISHI Ryo, KITADA Yuji, KASAHARA Michiko, HASHIZAKI Fumitaka, WADA Seitaro, MATUNAGA Masayuki, TAKAI Susumu, Japanese Journal of Zoo and Wildlife Medicine, 14, (1) 77 - 80,   2009 , 10.5686/jjzwm.14.77, http://hdl.handle.net/10659/2422, Urinary 8-hydroxyguanosine (8-OHdG), a common biomarker of oxidative stress, was measured in 9 captive western lowland gorillas (Gorilla gorilla gorilla) by ELISA kit. The data showed that urinary 8-OHdG ranged from 4.3 to 193.1ng/mg creatinine. An individual range of median value was 6.8-52.4ng/mg creatinine. No statistically significant effect was found for infection of protozoan parasites (Balantidium coli and Isospora sp.) without any symptoms (>0.05).
牛の内科実習 第76回 黒毛和種牛のボルナ病, 染谷勇介, 小岩政照, 鈴木一由, 田口清, 萩原克郎, 福永成己, 臨床獣医, 26, (10) 46 - 50,   2008 10 01 , http://jglobal.jst.go.jp/public/200902259635265359
母牛の産歴における黒毛和種子牛の免疫機能の比較, 中山哲也, 田原口貞生, 上野大作, 加藤(森)ゆうこ, 折橋毅典, 萩原克郎, 北海道獣医師会雑誌, 52,   2008 08 01 , http://jglobal.jst.go.jp/public/200902290212012262
Bovine colostral CD8-positive cells are potent IFN-gamma-producing cells., Katsuro Hagiwara, Mayumi Domi, Junichi Ando, Veterinary immunology and immunopathology, 124, (1-2) 93 - 8,   2008 07 15 , Refereed, 10.1016/j.vetimm.2008.02.018, IFN-gamma plays an important role in cellular immunity contributing to microorganism elimination. We have previously reported that bovine colostrum contains high levels of IFN-gamma as well as immunoglobulin. Lymphocytes are potent producers of IFN-gamma, so establishing the lymphocyte population in colostrum will help to identify the source of colostral cytokines. In this study, we used flow cytometric analysis to quantify the population of three types of lymphocytes found in colostrum; namely, CD4 (Th) cells, CD8 (cytotoxic T) cells, and gammadelta-T cells. We also quantified the concentration of colostral IFN-gamma using ELISA. IFN-gamma concentrations were measured in colostrum obtained from 96 healthy Holstein cows; the levels tended to decrease on the first day post-parturition. Flow cytometric analysis showed that many gammadelta-T- and CD8-positive cells were present in the colostrum, and that the CD4/CD8 ratio was low. The ratios of CD8- and gammadelta-T-positive cells to cells of other types decreased during the 5 days after parturition, but that of CD4-positive cells showed no change during the observation period. To identify IFN-gamma-expressing colostral lymphocytes, we used magnetic separation technology to separate the lymphocytes (CD4, CD8 and gammadelta-T) from colostral cells, then examined them for IFN-gamma mRNA expression with real-time PCR (RT-PCR). RT-PCR analysis revealed potent expression of the IFN-gamma gene in CD8-positive cells, reaching higher levels than in CD4- or gammadelta-T-positive cells. These results suggest that the CD8-positive T cells in colostrum play a role as producers of IFN-gamma.
Changes in Cytokine Concentrations in Serum and Whey from Dairy Cows with Peracute Mastitis Caused by Escherichia coli and Association with Clinical Findings, 久枝啓一, 有馬春樹, 園部隆久, 那須正信, 萩原克郎, 桐沢力雄, 岩井浤, 永幡肇, 日本獣医師会雑誌, 61, (6) 443 - 448,   2008 06 20 , 10.12935/jvma1951.61.443, http://jglobal.jst.go.jp/public/200902294339310284
Changes in Cytokine Concentrations in Serum and Whey from Dairy Cows with Peracute Mastitis Caused by Escherichia coli and Association with Clinical Findings, HISAEDA Keiichi, ARIMA Haruki, SONOBE Takahisa, NASU Masanobu, HAGIWARA Katsuro, KIRISAWA Rikio, IWAI Hiroshi, NAGAHATA Hajime, Journal of the Japan Veterinary Medical Association, 61, (6) 443 - 448,   2008 06 , 10.12935/jvma1951.61.443, http://ci.nii.ac.jp/naid/10025574175, This study was conducted to determine the concentrations of cytokines, interleukin (IL)-1β, IL-1 receptorantagonist (ra), IL-6, interferon (IFN)-γ and tumor necrosis factor (TNF)-α in serum and whey from clinicallyhealthy cows and cows with peracute coliform mastitis, and to evaluate the association of increased cytokinelevels in mastitic cows with their clinical findings. The concentrations of IL-1β, IL-6, and IFN-γ in serumand whey from <I>E. coli</I> mastitic cows at the onset of mastitis were apparently increased, compared with thoseof healthy cows. Increased levels of cytokine concentrations in whey were reduced during the mastitis recoveryprocess of. Correlations were found between increased levels of IL-6 in serum and whey and IFN-γ inwhey and their characteristic findings in <I>E. coli</I> mastitic cows at the day of diagnosis. These findings suggestthat increased levels of cytokine concentrations in dairy cows with <I>E. coli</I> mastitis may be associated with thepathogenesis and their characteristic findings.
An Epidemiological Survey of Hepatitis E virus in Sika Deer, Cervus nippon, in Nara Park, Japan, 萩原克郎, 辻正義, 川渕貴子, 鳥居春己, 小林朋子, 浅川満彦, 石原智明, 日本野生動物医学会誌, 13, (1) 35 - 37,   2008 03 , 10.5686/jjzwm.13.35, http://jglobal.jst.go.jp/public/200902218604749252
An Epidemiological Survey of Hepatitis E virus in Sika Deer, Cervus nippon, in Nara Park, Japan, HAGIWARA Katsuro, TSUJI Masayoshi, KAWABUCHI Takako, TORII Haruki, KOBAYASHI Tomoko, ASAKAWA Mitsuhiko, ISHIHARA Chiaki, Japanese Journal of Zoo and Wildlife Medicine, 13, (1) 35 - 37,   2008 03 01 , 10.5686/jjzwm.13.35, http://ci.nii.ac.jp/naid/110006781658, Hepatitis E Virus(HEV) infections have been reported in deer as well as in domestic animals; however, the precise epidemiological information regarding HEV infections in the Shika Deer in Nara Park in Japan remains to be investigated. In this study, we examined the anti-HEV antibodies and HEV-RNA in sera from 173 of female sika deer in the park. The reactivity to HEV antigen in the serum samples were low levels. The detection of HEV-RNA in sera from the deer revealed no positive samples by RT-PCR analysis. In conclusion, we could not able to identify the HEV antibody and HEV-RNA positive individual in the sika deer which inhabited Nara Park. Therefore, the possibility of the HEV infection in the deer of the park would be extremely low.
Borna disease virus RNA detected in Japanese macaques (Macaca fuscata)., Katsuro Hagiwara, Yusuke Tsuge, Mitsuhiko Asakawa, Hajime Kabaya, Minoru Okamoto, Taku Miyasho, Hiroyuki Taniyama, Chiaki Ishihara, Juan Carlos de la Torre, Kazuyoshi Ikuta, Primates; journal of primatology, 49, (1) 57 - 64,   2008 01 , Refereed, 10.1007/s10329-007-0068-8, We have examined the seroprevalence of BDV in wild Japanese macaques (Macaca fuscata) in the peninsula (Chiba prefecture), Japan. Serum samples from macaques were examined by the ELISA, Western blot and immunofluorescence assays to detect the presence of serum antibodies that react specifically to BDV antigens. Among 49 investigated individuals, 6 (12.2%) showed positive reaction to BDV antigens. RT-PCR studies detected BDV sequences in brain tissue of one case among four seropositive cases examined. Sequence analysis revealed a high degree of genetic conservation between BDV sequences derived from Japanese macaques and those documented for other animal species. Nevertheless, phylogenetic analysis revealed unique differences between macaque and other species derived BDV sequences.
ブタの胆管周囲嚢胞と胆管嚢胞における病理組織学的検索, 小嶺美紗, 岡本実, 溝奥尋子, 高田豊樹, 川迫一史, 周本剛大, 萩原克郎, 谷山弘行, 北海道獣医師会雑誌, 51,   2007 08 01 , http://jglobal.jst.go.jp/public/200902288316321602
輸入牧草に混入し北海道にてその死体が確認されたホシムクドリSturnus vulgarisの記録とウイルス学的検査, 吉野智生, 国藤泰輔, 渡辺竜己, 久木田優美, 前田秋彦, 萩原克郎, 大沼学, 桑名貴, 村田浩一, 浅川満彦, 北海道獣医師会雑誌, 51, (3) 68 - 70,   2007 03 01 , http://jglobal.jst.go.jp/public/200902257435271378
腫瘍疾患を有する犬におけるリンパ球分画の動態, 山本竜太, 三輪祐一郎, 萩原克郎, 廉澤剛, 北海道獣医師会雑誌, 50,   2006 08 01 , http://jglobal.jst.go.jp/public/200902262387199566
牛群検診で高体細胞を呈する乳牛に対するステビア抽出発酵液経口投与の効果, 酒見蓉子, 青木ひとみ, 藤平ひとみ, 小岩政照, 萩原克郎, 澤向豊, 北海道獣医師会雑誌, 50,   2006 08 01 , http://jglobal.jst.go.jp/public/200902221030929126
乳房炎の新知見―ここまで進んだ乳房炎研究―サイトカインと予後診断, 萩原克郎, 獣医畜産新報, (1019) 453 - 457,   2006 06 01 , http://jglobal.jst.go.jp/public/200902261291355404
Development of a sandwich ELISA assay for measuring bovine soluble type II IL-1 receptor (IL1R2) concentration in serum and milk., Katsuro Hagiwara, Keiko Kitajima, Hitoki Yamanaka, Rikio Kirisawa, Hiroshi Iwai, Cytokine, 32, (3-4) 132 - 6,   2005 11 03 , Refereed, 10.1016/j.cyto.2005.08.007, The IL1R is composed of two kinds of molecule, type I (IL1R I) and type II (IL1R2). IL1R1 contributes to IL-1 signaling, whereas the IL1R2 has no signaling property and acts as a decoy for IL-1. In this study, we developed a bovine IL1R2-specific sandwich ELISA to examine the sIL1R2 concentration in serum and milk from dairy cows. The concentration of colostral IL-1beta was examined to estimate the correlation to sIL1R2. The results showed that the sIL1R2 concentration in sera and milk changes with the stages of lactation. The serum sIL1R2 concentrations were 5.56+/-0.69 ng/ml (colostrum), 3.14+/-0.72 ng/ml (the early stage of lactation) and 5.76+/-1.25 ng/ml (the late stage of lactation). The milk sIL1R2 concentrations were 1.83+/-0.47 ng/ml (colostrum), 0.73+/-0.22 ng/ml (the early stage of lactation) and 2.92+/-0.56 ng/ml (the late stage of lactation). The concentrations of IL1R2 in sera and milk were significantly higher at the late stage of lactation and colostrum than that of the early stage of lactation. The reduction rates of sIL1R2 levels from the colostrum to the early stage of lactation were 43.6% (serum) and 61% (whey). IL-1beta was detected in all the colostrum (995.9+/-346.6 ng/ml). Significant correlation was observed between the levels of colostral IL-1beta and IL1R2 (r=0.75).
ボルナ病ウイルス感染牛群における臨床および繁殖調査成績, 三好正一, 萩原克郎, 小岩政照, 青木創, 虻川孝秀, 望月誠, 山中栄, 北海道獣医師会雑誌, 49,   2005 08 01 , http://jglobal.jst.go.jp/public/200902227189128475
敗血症小動物モデル重症化における経時的採血の影響とその対策, 須田康一, 北川雄光, 小沢壮治, 才川義朗, 上田政和, 矢野和仁, 大山隆史, 小熊潤也, 田淵悟, 安藤崇史, 宮庄拓, 萩原克郎, 横田博, 石坂彰敏, 北島政樹, 外科と代謝・栄養, 39,   2005 06 15 , http://jglobal.jst.go.jp/public/200902231199582619
Serum cytokine levels evaluated in neonatal calves in relation to disease incidence up to 4 months after colostrum ingestion, 平子毅, 久枝啓一, 泉賢一, 黒沢隆, 萩原克郎, 桐沢力雄, 岩井ひろし, 酪農学園大学紀要 自然科学編, 29, (2) 171 - 175,   2005 04 20 , http://jglobal.jst.go.jp/public/200902278175899570
Serum cytokine levels evaluated in neonatal calves in relation to disease incidence up to 4 months after colostrum ingestion, HIRAKO Tsuyoshi, HISAEDA Keiichi, IZUMI Kenichi, KUROSAWA Takashi, HAGIWARA Katsuro, KIRISAWA Rikio, IWAI Hiroshi, Journal of the College of Dairying. Natural science, 29, (2) 171 - 175,   2005 04 , http://ci.nii.ac.jp/naid/110002970372
The role of IFN-gamma in cattle infected with Theirelia sergenti, K Hagiwara, M Tokuda, T Baba, H Yamanaka, R Kirisawa, M Tsuji, C Ishihara, F Iwai, VETERINARY PARASITOLOGY, 127, (2) 105 - 110,   2005 01 , 10.1016/j.vetpar.2004.09.022, The spleen plays an important role in cellular immunity leading to parasite elimination, especially in hemG-protozoan parasite infection. In the present study, we quantified the serum IFN-gamma in splenectomized (SP) and non-splenectomized (NSP) cows infected with Theirelia sergenti (TS) to investigate whether the spleen plays a role in the Th1-type cytokine responses in cows following parasite infection. A transient increase in IFN-gamma was observed in the early stages of infection in the NSP cowl., and the cows did not develop parasitemia. In contrast, the SP cows showed no IFN-gamma response at the early stage of infection. and the cows developed parasitemia following anemia. The NSP cows produced IgG1 and IgG2 antibodies to TS-specific. whereas the SP cows showed increases only in IgG1. Increased PBMC proliferation by cocanavalin A stimulation was observed concomitant with the IFN-gamma response. Pretreatment with IFN-gamma suppressed the propagation of TS in the SP cattle. These results suggest that the spleen plays an important role in the resistance to TS infection. leading to a Th1-type immune response. IFN-gamma might contribute to the activation of immunocytes and play an important role in the immune response to resist TS proliferation. (C) 2004 Elsevier B.V. All rights reserved.
Changes in Interleukin-6 Concentration in Peripheral Blood of Pre- and Post-Partum Dairy Cattle and Its Relationship to Postpartum Reproductive Diseases(Theriogenology), ISHIKAWA Yukikazu, NAKADA Ken, HAGIWARA Katsuro, KIRISAWA Rikio, IWAI Hiroshi, MORIYOSHI Masaharu, SAWAMUKAI Yutaka, The journal of veterinary medical science, 66, (11) 1403 - 1408,   2004 11 25 , 10.1292/jvms.66.1403, http://ci.nii.ac.jp/naid/110003963621, Reproductive diseases after parturition are a serious problem in dairy cattle. It is important to predict postpartum reproductive diseases early and to develop prophylaxis. The objectives of this study were to demonstrate changes in the peripheral blood concentration of interleukin-6 (IL-6) before parturition, which was mainly produced by T helper 2 type (Th2) cells, and to investigate a correlation between the IL-6 concentration and the occurrence of the postpartum retained placenta, endometritis and/or follicular cyst in dairy cattle. Twenty-seven Holstein-Friesian cows were used for this study. Thirteen had no clinical disease, 8 had retained placenta, 4 were diagnosed with endometritis by vaginal inspection, and 2 were diagnosed with follicular cyst by rectal palpation at 1 and 2 months after parturition. Blood samples were collected 60 days pre- and post-partum. They used for IL-6, progesterone (P_4) and estradiol-17β (E_2) concentration determination. This study showed that the IL-6 concentration prepartum was higher than postpartum. Low levels of IL-6 and P_4 in peripheral blood prepartum tended to affect retained placenta and a high level of IL-6 prepartum tended to affect endometritis. These results indicate that measurement of change in the IL-6 concentration during pregnancy is one useful tool for predicting crisis in postpartum reproductive diseases in dairy cattle.
A Preliminary Report of Parasitological and Microbiological survey of Freeranging Japanese Macaques (Macaca fuscata (Blyth)) in Boso Peninsula, Japan, SATOYOSHI Ayako, KABAYA Hajime, HAGIWARA Ko, TANIYAMA Hiroyuki, YOSHIZAWA Kazunori, TUJI Masayoshi, HAGIWARA Kasturo, MURAMATSU Yasukazu, ASAKAWA Mitsuhiko, Japanese Journal of Zoo and Wildlife Medicine, 9, (2) 79 - 83,   2004 09 01 , 10.5686/jjzwm.9.79, http://ci.nii.ac.jp/naid/110002665451, Because alien macaques (probably, Macaca mulatta) occur in the southern part of the Boso Peninsula, Japan, there is a possibility that their parasites and/or other pathogens may infect Japanese macaques (M.fuscata). To verify the possibility, this study was conducted mainly on parasites that included protozoan, helminth and arthropod, and other pathogens including Rickettsia and the Borna virus of 50 free ranging Japanese macaques in the peninsula. Consequently, 5 arthropod and nematode parasite species common to the Japanese macaque, namely Pedicinus obtusus, P.eurygaster, Streptopharagus pigmentatus, Strongyloides fulleborni, and Trichuris trichiura, were obtained in this survey. It is the first record of sites where Pedicinus spp. are seen in a host species.Coccidia and Babesia sp. were not obtained. Coxciella and the Borna virus from the same host samples will be published elsewhere.
Epizootiologic Survey for Babesia microti among Small Wild Mammals in Northeastern Eurasia and a Geographic Deversity in the β-Tubulin Gene Sequences(Public Health), ZAMOTO Aya, TSUJI Masayoshi, WEI Qiang, CHO Shin-Hyeong, SHIN E-Hyun, KIM Tong-Soo, LEONOVA Galina N, HAGIWARA Katsuro, ASAKAWA Mitsuhiko, KARIWA Hiroaki, TAKASHIMA Ikuo, ISHIHARA Chiaki, The journal of veterinary medical science, 66, (7) 785 - 792, s-vii-s-viii,   2004 07 25 , 10.1292/jvms.66.785, http://ci.nii.ac.jp/naid/110003960139, We previously reported that small wild rodents in Japan harbor two types of novel Babesia microti-like parasites(designated as Hobetsu and Kobe types), but not the type commonly found in the northeastern United States(U. S. type) where human babesiosis is endemic. To determine whether these new types of parasites are distributed in places surrounding Japan, an epizootiologic survey was undertaken in three geographically distant areas in northeastern Eurasia ; South Korea, Vladivostok in Russia, and Xinjiang in China. Blood samples were collected from a total of 387 animals comprising 24 species. DNAs extracted from the samples were tested by nested PCR targeting babesial nuclear small-subunit rRNA gene(rDNA), which revealed that small rodents harboring B. microti exist in all three survey areas. Sequence analysis showed that all PCR-positive samples had rDNA sequences virtually identical to that of U. S.-type B. microti. However, when β-tubulin gene sequences were compared, evident geographic variations were seen. By use of primers specific for each of the β-tubulin genes of Kobe-, Hobetsu-, and U. S.-type parasites, a type-specific PCR was developed. Parasite with Hobetsu- or Kobe-type sequence was not detected from any of the three survey areas. These findings suggest that U. S.-type B. microti is widely distributed among small wild mammals in temperate zones of not only North America, but also Eurasia, whereas that Hobetsu- and Kobe-type parasites may be uniquely distributed in Japan.
脳に感染するウイルス―日本におけるボルナ病ウイルスについて―, 萩原克郎, 酪農ジャーナル, 56, (7) 33 - 35,   2003 07 01 , http://jglobal.jst.go.jp/public/200902213357486381
Cytokine Profile of a Holstein Calf with Bovine Leukocyte Adhesion Deficiency during the Acute-Phase Inflammatory Response(Immunology), NAGAHATA Hajime, HAGIWARA Katsuro, KASAMATSU Masahiko, HIGUCHI Hidetoshi, KUROSAWA Takashi, The journal of veterinary medical science, 64, (12) 1141 - 1143,   2002 12 25 , 10.1292/jvms.64.1141, http://ci.nii.ac.jp/naid/110003886083, Changes in interleukin (IL)-1β, IL-6 and IL-8 in serum, and their mRNA expression on neutrophils from a 4.6-month old Holstein young calf with bovine leukocyte adhesion deficiency (BLAD) during the acute phase were evaluated. IL-1β concentrations in the serum of the calf with BLAD at age 143-162 days ranged from 8.7 to 16.6 ng/ml, whereas the values were less than 2.7 ng/ml in control calves. Serum IL-6 (0.04 ng/ml) was only detected on the 1st day when the animal was diagnosed with the BLAD. IL-1β and IL-8 mRNA expression on neutrophils from the affected calf appeared to be similar to those of controls. Serum cysokine levels and their mRNA expression on neutrophils from the calf with BLAD appeared to be little affected by the deficient expression of 132-integrin on leukocytes, and are considered to be modulated by the inflammatory stimuli.
Analysis of Serum Cytokine Profile in a Holstein Heifer with Leukocyte Adhesion Deficiency Which Survived for Long Period(Clinical Pathology), NAGAHATA Hajime, HAGIWARA Katsuro, HIGUCHI Hidetoshi, KIRISAWA Rikio, IWAI Hiroshi, The journal of veterinary medical science, 64, (8) 683 - 687, s・iv,   2002 08 25 , 10.1292/jvms.64.683, http://ci.nii.ac.jp/naid/110003920898, Serum cytokine levels and their expression of mRNA on neutrophils from a bone marrow (BM) transplanted heifer with leukocyte adhesion deficiency were evaluated. The clinical condition of the affected heifer was relatively stable after BM-transplantation. Persistent hyper γ-globulinemia and increased serum immunoglobulin G (IgG) concentrations were monitored longitudinally. The concentration of interleukin (IL)-1β in serum from the affected heifer ranged from 15.8 to 321.7 ng/ml, and maximum concentration occurred at the time which coincided with peak IL-6. Serum levels of IL-6 ranged from 0.32 to 27.9 ng/ml, and they appeared to be associated with the increment of serum IgG in the affected heifer. mRNAs for IL-1β, IL-6, IL-8 and granulocyte and macrophage colony stimulating factor (GM-CSF) were increased in neutrophils from the affected heifer compared to controls. Persistent hyper γ-globulinemia of the affected heifer appeared to be associated with enhanced mRNA expression for IL-6 and its serum levels. These findings suggest that humoral immunity of the affected heifer is activated and the production of neutrophils appears to be enhanced under the incapability of β_2 integrin-mediated functions of phagocytic cells.
新生子牛血清中サイトカイン動態とその意義, 山中仁木, 萩原克郎, 桐沢力雄, 岩井ひろし, 北海道獣医師会雑誌, 46,   2002 08 01 , http://jglobal.jst.go.jp/public/200902119911389516
ボルナ病発生牛のボルナ病ウイルス垂直感染, 岡本実, 小岩政照, 古岡秀文, 萩原克郎, 桐沢力雄, 岩井ひろし, 鈴木隆秀, 谷山弘行, 北海道獣医師会雑誌, 46,   2002 08 01 , http://jglobal.jst.go.jp/public/200902186707353960
Interferon-γ and Tumor Necrosis Factor-α Levels in Sera and Whey of Cattle with Naturally Occurring Coliform Mastitis, Keiichi Hisaeda, Katsuro Hagiwara, Junko Eguchi, Hitoki Yamanaka, Rikio Kirisawa, Hiroshi Iwai, Journal of Veterinary Medical Science, 63, (9) 1009 - 1011,   2001 09 , 10.1292/jvms.63.1009, http://ci.nii.ac.jp/naid/110003920736, Concentrations of interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) were determined in serum and whey samples from cattle with naturally occurring coliform mastitis for two weeks after onset using bovine INF-γ- and TNF-α-specific ELISA. In serum and whey samples from healthy cows, IFN-γ was almost undetectable and TNF-α was detected at low levels. At the onset of illness, INF-γ in sera and whey and TNF-α in whey from the mastitic cows were significantly higher than their respective values in healthy cows. Concentrations of IFN-γ and TNF-α in whey from mastitic cattle decreased significantly as the cows recovered.
乳房炎り患時における臨床症状と炎症性サイトカインの動態, 秦秀明, 岡井和彦, 田原口貞夫, 萩原克郎, 北海道獣医師会雑誌, 45,   2001 08 01 , http://jglobal.jst.go.jp/public/200902130379634446
Effect of interleukin-1 receptor antagonist on concanavalin A response of peripheral blood mononuclear cells from newborn calves, H Yamanaka, Y Katagiri, M Domi, K Hagiwara, R Kirisawa, H Iwai, JOURNAL OF VETERINARY MEDICAL SCIENCE, 63, (8) 933 - 935,   2001 08 , 10.1292/jvms.63.933, http://ci.nii.ac.jp/naid/110003920721, The coexistence of interleukin (IL)-1 beta with IL-1 receptor antagonist (ra) in bovine colostrum and the possibility of simultaneous transfer of these cytokines to neonates via colostrum have been demonstrated. In the present study, we investigated the effect of IL-1ra on the mitogenic response of calf peripheral blood mononuclear cells (PBMC) stimulated by concanavalin A (ConA), which was mediated by IL-1. Pretreatment of PBMC with recombinant bovine (rb) IL-1ra alone significantly suppressed the proliferation of ConA-stimulated cells. However, in the presence of rbIL-1 beta, the suppressive activity of rbIL-1ra was counteracted. These results suggest that coexistence of IL-1ra with IL-1 in colostrum may have no effect on the activation of the neonatal immune system by IL-1 beta.
わが国における馬ボルナ病の初発生例, 岡本実, 萩原克郎, 桐沢力雄, 岩井ひろし, 谷山弘行, 北海道獣医師会雑誌, 45, (7) 205 - 207,   2001 07 01 , http://jglobal.jst.go.jp/public/200902197325119888
ELISA for bovine interleukin-1 receptor antagonist and its application to mastitic sera and whey, H Yamanaka, K Hisaeda, K Hagiwara, R Kirisawa, H Iwai, JOURNAL OF VETERINARY MEDICAL SCIENCE, 62, (6) 661 - 664,   2000 06 , 10.1292/jvms.62.661, http://ci.nii.ac.jp/naid/110003920385, A sandwich ELISA for the bovine IL-1 receptor antagonist (bIL-1ra) was developed using recombinant (r) bIL-1ra produced by Escherichia coli, anti-rbIL-1ra rabbit IgG, its biotinylated one and avidin-peroxidase. This ELISA system enabled detection of rbIL-1ra at a concentration of more than 2 ng/ml. This ELISA was applied to quantitation of bIL-1ra in sera and whey of mastitic and healthy cows. The results indicate thar although IL-1ra levels in healthy and mastitic sera and whey were comparable, serum IL-1ra/IL-1P beta ratio of euthanized cows was significantly lower than that of the recovered.
A method for analysis of cell to cell transmission of Theileria sergenti piroplasms, Y Terada, M Tsuji, Y Nakamura, K Hagiwara, K Takahashi, C Ishihara, JOURNAL OF VETERINARY MEDICAL SCIENCE, 60, (1) 115 - 117,   1998 01 , 10.1292/jvms.60.115, http://ci.nii.ac.jp/naid/110003918388, We tried to develop an experimental system for quantifying piroplasm transmission in Theileria sergenti infection using a SCID mouse model, of which red blood cells (RBC) had been substituted with bovine (Bo). A mouse hybridoma cell producing antibody against Holstein RBC was established for distinguishing Holstein RBC from Japanese Black RBC by a immune-staining method. T. sergenti piroplasms in RBC were stained by 4',6-diamidino-2-phenylindole. With the aid of these techniques the piroplasm transmission from one to another race of Bo-RBCs could successfully be quantified in SCID mice.
Studies on an experimental animal model for Theileria sergenti infection, HAGIWARA Katsuro, Journal of the College of Dairying. Natural science, 20, (1) 131 - 132,   1995 10 , http://ci.nii.ac.jp/naid/110000363115
TRANSFUSION WITH XENOGENEIC ERYTHROCYTES INTO SCID MICE AND THEIR CLEARANCE FROM THE CIRCULATION, C ISHIHARA, M TSUJI, K HAGIWARA, K HIOKI, J ARIKAWA, AZUMA, I, JOURNAL OF VETERINARY MEDICAL SCIENCE, 56, (6) 1149 - 1154,   1994 12 , 10.1292/jvms.56.1149, http://ci.nii.ac.jp/naid/110003916178, We have previously demonstrated that the red blood cells (RBCs) in the blood circulation of SCID mice could be almost completely substituted with bovine RBCs by means of repeated transfusions. In the present study, transfusion experiments were carried out with RBCs from various animal species to investigate the ability of SCID mice to accept xenogeneic RBCs. Bovine (Bo), equine (Eq), human (Hu) and murine (Mu) RBCs were labeled with a fluorescent dye to trace their trafficking in the blood stream. Following the intravenous injection these RBCs were cleared from the circulation at various rates; the 1/100 reduction time in the labeled cell counts was 2 to 7 days, 4 to 7 hr and 1 to 2 hr with Bo-, Hu- and Eq-RBCs, respectively. In contrast, labeled Mu-RBCs from not only syngeneic but also allogeneic mouse strain were able to stay over 50 days. The difference in clearance rates was attributable to the difference in uptake of the RBCs by the splenic macrophages. The clearance rates of labeled RBCs were significantly decreased by co-transfusion with unlabeled RBCs of the same species, indicating that xenogeneic RBCs were recognized by the reticulo-endothelial system of SCID mice in a species-specific fashion. Furthermore, at least in the case of Hu-RBC, the complement component 3 may play a role in the Hu-RBC clearance in SCID mice since C3 deposition was observed on Hu-RBCs but not on Bo- and Eq-RBCs.
THE DETECTION OF A MUTATION OF CD18 GENE IN BOVINE LEUKOCYTE ADHESION DEFICIENCY (BLAD), M TAJIMA, M IRIE, R KIRISAWA, K HAGIWARA, T KUROSAWA, K TAKAHASHI, JOURNAL OF VETERINARY MEDICAL SCIENCE, 55, (1) 145 - 146,   1993 02 , 10.1292/jvms.55.145, http://ci.nii.ac.jp/naid/110003915772, Two calves (5 and 9 months old) affected with pneumonia and gingivitis were also diagnosed as having bovine leukocyte adhesion deficiency (BLAD). The gene of leukocyte adhesion molecule CD18 in these BLAD calves and their dams (carrier) were examined by means of polymerase chain reaction (PCR) and digestion of restriction endonuclease. The splicing in mRNA coded CD 18 reported in human LAD was not recognized in BLAD on the basis of the results of PCR amplification. The region including the portion of point mutation, which corresponded to the region reported in the human patient, was amplified by PCR, and the PCR product was then digested with TaqI. An obvious difference was recognized in the pattern of digestion among healthy calves, BLAD calves and their dams. In BLAD, therefore, the point mutation reported in human patients was strongly suggested. Moreover it may be a method able to be used in detecting the carrier.

Research Grants & Projects

Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Elucidation of sex hormone secretion dynamics in Borna disease virus-1 persistently infected animals
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Impact assessment for air pollutants affecting infectivity of bioaerosols and disease prevention, This work tried to understand the mechanism and linkage between soot particles and bioaerosols, which containing bacteria or virus, to minimize an adverse health effect associated with airborne infectious diseases. We have targeted the soot as a model air pollutant, which often generated from incomplete combustion of various fuels, and ozone as environmental stress factor to measure the effect on the viability of bacteria in a simulation reaction chamber. From the result, the soot and bacteria co-existing condition showed less influence of ozone stress. Furthermore, we have demonstrated that examination of soot and/or other organic compounds to contribute to maintaining the viability of airborne bacteria.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Control of bovine viral diarrhea virus infection as a latent disease: preventive investigation for the transmission, In order to estimate the spread manner of bovine viral diarrhea virus (BVDV) in and among the dairy herd, epidemiological and immunological analyzes were performed for the BVDV detected and free herd discriminated by the bulk tank milk tests and the mass surveillance. The results indicated that in-uterus persistently infected fetus moved as pregnant cow with the dam among the herd, then the new prevalence was confirmed. It seemed to be important for the prevention to keep the BVDV-free status besides vaccination.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research, Bioaerosol time-space transition during Asian dust transport incorporating cloud physics process, We investigated the spatio-temporal variation of bioaerosol occurred in the Mongolian Gobi Desert. The asian dust which occurred in the Mongolian Gobi desert arrived in Hokkaido area through the Westerly Wind without spreading in the meridian direction, according to meteorological model. We were also able to confirm occurrence / transport of Asian dust from LIDAR (Light Detection and Ranging, Laser Imaging Detection and Ranging) and artificial satellites CALIPSO. From aerosol samples acquired in Mongolian Gobi desert and Sapporo, they were biologically identified between them.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Research of phage therapy and phage vaccine for bacterial infectious diseases., Antimicrobial resistance in bacteria is a concern not only in veterinary medicine, but also in general, worldwide. There is a demand for new strategies to treat bacterial infections. In this research, we investigated the ‘phage therapy for bacterial infection’ and ‘phage vaccine’. We examined the use of phage to treat horse bacterial keratitis as a research of phage therapy. We isolated phages toward P. aeruginosa causing horse keratitis. The phages in this study could completely prevent the keratitis caused by P. aeruginosa in a keratitis mouse model. In the phage vaccine, we constructed phage with surface antigen of Mycoplasma.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research, Investigation of tsunami deposit as a source of airborne dust and its potential effect and prevention for infection by bioaerosols, Dry marine sediment and sludge deposit may become a source of suspended particulate matter (SPM) after the tsunami disaster in 2011, Japan. Further understanding of interaction between SPM and bioaeosol in the atmosphere may contribute to prevent spread of pathogenic microbes through air. This work investigates the effect of marine deposits and some other type of SPM to attribute a survival of biological aerosol in the tropospheric condition. Experiments were carried out with a reaction chamber to examine different types of SPM and bioaerosol in a laboratory. Results indicated that the SPM of marine deposit and model enteric bacteria had significantly higher survival rate than other SPMs examined. The different SPMs may play a significant role for the survival of airborne bacteria and other pathogenic microbes. It is necessary to further investigate the interactions between SPM and bioaerosols to understand factor influencing spread of pathogens to safeguard public health.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Epidemiological study on Hepatitis E virus in wild and domestic animals, We conducted an epidemiological survey of the hepatitis E virus in the wild animal. As a result, a HEV-RNA was detected in approximately 47% of individual livers out of 629 Yezo deer (Cervus nippon yesoensis) which we investigated and showed a viral gene in 12% more individual feces. Also, after investigating the palm civet (Paguma larvata) which inhabited Honshu, we detected the viral genome of 30% in feces, 53% in liver. It was suggested that a HEV habitually resided to these wild animals. Also, the deer HEV was transmitted by the experimental infection to a pig, and HEV was isolated from the feces of some individuals and confirmed infectious viral excretion from the infected deer.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Investigation for pathogenesis of influenza virus and SARS coronavirus, The unprecedented re-emergence of highly pathogenic avian influenza H5N1 viruses (H5N1-Flu) and the outbreak of severe acute respiratory syndrome coronavirus (SARS-CoV) pose a significant threat to humans. Better basic knowledge of these highly pathogenic viruses and novel technologies such as reverse genetics will help us to generate effective prophylactic and/or therapeutic strategies to control the disease load. We have developed the reverse genetics of negative strand RNA viruses and were analyzing the mechanisms behind the replication and cytopathicity of these viruses. Primary airway epithelial cells from alveolar tissues were prepared from 1-year-old pigs and the growth kinetics of avian H5 influenza viruses in these cells was investigated. H5N1 virus significantly induced cell death, especially apoptosis, in porcine airway epithelial cells although these three viruses induced similar cell toxicity in chicken embryonic fibroblasts, suggesting that avian H5N1 influenza virus leads severe cell death in mammalian airway epithelial cells due to the induction of apoptosis. A cell clone #21 is a long-term producer of the infectious SARS-CoV, although the incorporation rate of spike (S) protein into virions is significantly lower. Sequencing analysis of the viral structural proteins revealed four and one amino acid substitutions in the S and membrane (M) proteins, respectively. We demonstrated, using a viral-like particle formation system, that the S mutations were involved in lower incorporation of the S protein into virions, although the M mutation disrupting the glycosylation was not involved in this phenotype. Further mutational experiments identified two substitutions, Y442C and L472F, within the receptor binding domain that could be critical for the reduced S incorporation, as well as reduced binding affinity between the S and ACE2 receptor. Thus, these two amino acid substitutions might lead to a conformational change in the S protein, resulting in reduced incorporation into viral particles.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Experimental transmission of abnormal prion protein (PrPsc) in the small intestinal epithelial cells of neonatal mice, Using an immunohistochemical method, we attempted to detect the transmission of abnormal prion protein (PrPsc) to the enterocytes of the small intestine of neonatal mice by oral exposure with sheep brain affected by scrapie. Five 1-day-old neonatal mice were exposed by oral inoculation to the homogenized brain of a scrapie-affected sheep. In the small intestine of all mice 1 hour after inoculation, immunoreactivity with antinormal prion protein (PrPc) antibody was seen in the cytoplasm of viilus enterocytes. This finding suggests transmission of abnormal PrPsc into the cytoplasm of enterocytes. In control mice treated with normal sheep brain, no PrPc signal was seen in enterocytes of the small intestine. Immunopositivity for neurofilament protein and glial fibrillary acidic protein was seen in the cytoplasm of enterocytes of mice inoculated with scrapie and normal sheep brain. This suggests that the enterocytes of neonatal mice can absorb PrPsc and other macromolecular proteins of the sheep brain affected by scrapie and may be more important than previously thought as a pathway for PrPsc transmission in neonatal animals. After 7 days to 3months of oral inoculation to the homogenized brain of a scrapie-affected sheep, immunoreactivity with antinormal prion protein (PrPc) antibody was not seen in the cytoplasm of villus enterocytes and other tissues in the mucosa. The results suggest that the term of the transmission of abnormal priors protein (PrPsc) ineeds over 3 months and another system of the transmission is present in the mucosa of mouse. Lam inine reseptor (LR) and normal prion were more frequently present in the cytoplasm of enterocytes of suckling mice and young cattle than adult mice and cattle, thus young animals have the higher risk of transmission of abnormal prion than adult animals, via the gastrointestinal menbrane.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Study on Persistent infection of Borna Disease Virus in Brain, The interferon (IFN)-induced proznyelocytic leukemia (PML) protein is associated with nuclear bodies (NBs) whose functions remain poorly understood. Overexpression of PML has been shown to affect the replication of several RNA viruses, including influenza and the complex retrovirus human foamy virus (HFV). Various viruses induce disorganization of the PML NBs, which is thought to be part of a general viral strategy to counteract IFN action. The mononegavirus Boron disease virus (BDV) can persistently infect neurons and astrocytes in the rat central nervous system (CNS) in the presence of a robust IFN response. Replication and transcription of BDV occur in the nucleus, a unique feature among known animal mononegaviruses. BDV antigens colocalize with PML, and persistence of BDV induces PML NBs disorganization suggesting a possible mechanism whereby BDV counteract the IFN response. Vero cells do not produce IFN, hence facilitating the evaluation of PML antiviral activity in the absence of other IFNinduced genes. Overexpression of PML via a recombinant adenovirus (AdPML) induced high levels of PML NBs structures in Vero cells, but did not exert any antiviiral activity against BDV Virus titers, as well as levels of BDV replication and transcription were similar in Vero cells that were infected with AdPML or mock-infected prior infection with BDV. In contrast, and consistent with a previous report, infection with AdPML resulted in about 1000-fold decrease in the production of infectious VSV. NBs have been implicated in hormone signaling, and glucocorticoids are potent modulators of astrocyte function. The resistance of BDV to the antiviral action of PML together with BDV mediated disorganization of PML NBs may contribute to disturbances in CNS function associated with BDV persistence. PML is required for p53 pro-apoptotic signaling, as well as growth arrest inducing pathways. BDV persistence provides increased resistance to program cell death following exposure to Camptothecin. This effect was associated with impaired expression of the pro-apoptotic genes Caspase 3 and Bax. Protection from apoptosis was also observed in cells transfected with BDV N, Together these findings suggest that BDV N might be a repressor of apoptosis, an activity that could contribute to the exquisite ability of BDV to persist in brain cells.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), A BASIC APPROACH TO ASSOCIATION OF INSULIN RESISTANCE WITH TNF α IN COWS WITH FATTY LIVER, It has been recently reported that cows with insulin resistance are sometime observed during the periparturient period, and their milk production are remarkably decreased. It is pointed out that fatty liver is closely linked to that pathogenesis. Tumor necrosis factor ct (TNFα) secreted from fatty cell is considered to be the most important factor in the insulin resistance. We designed two experiments for induction of fatty liver. In experiment-1, cows were fasted for 4 day to be fallen into negative energy balance. Moreover, as experiment-2, cows were administrated ethionine, analogous substance of methionine, to reduce the production of proteins in the liver. Insulin resistance and TNF α of used cows were analyzed in each experiment. Experiment-1 1. Liver triglyceride (TG) content was apparently increased after fasting. 2. The increase of insulin resistance was observed in the development of fatty liver. 3. Serum concentration of TNFα was not significantly changed in the fasted cow. 4. In micromorphological analysis of liver, hepatic basal lamina in the cow with fatty liver remarkably became thicker compared to that in control. Experiment-2 1. Liver TG contents were remarkably increased in ethionine-treated cows. 2. Serum concentration of apolipoprotein B-100 was significantly decreased in the development of fatty liver. 3. Hepatic lipidosis apparently induced insulin resistance. 4. Hyperglycemia and hyperinsulinism were observed in treated cows. 5. The apparent association of fatty liver with serum concentration of TNFα was not detected. From these results, two experiments were suggested to be effective for induction of fatty liver. Although TNFα of sampled liver tissues were analyzed biochemically and histologically, its relevance to hepatic lipidosis was not detected. We are planning to improve TNFα determination and histological specimens to scrutinize these experiments.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), INCOORDINATION AND LOCALIZATION OF BORNA DISEASE VIRUS (BDV) IN THE BRAIN OF HORSES WITH BDV PERSISTENT INFECTION, 1.To investigate the prevalence of diseases in the Borna disease virus (BDV) antibody positive race horses, we undertook seroepidemiological studies of BDV infection on 125 culled race horses in Hokkaido, Japan. The serological study disclosed the presence of antibodies only to BDV- p40 or -p24 in 19.2 % (24/125) and 3.2 % (4/125) of culled horses, respectively. Antibodies to both BDV -p40 and -p24 were found in 24.0 % (30/125) of these horses. Of particular note was the finding that locomotorium disorders were detectable at a significantly higher rate in BDV antibody positive horses than that in the seronegative horses. These results imply that BDV infection may possibly contribute to an increase in the incidence rate of locomotorium disorders in racehorses. 2.Three central nervous systems collected from three horses with clinical wobbler disease and the Borna disease virus (BDV) positive antibody were investigated histopathologically. Immunohistochemical and in situ PCR examinations were performed for BDV antigens and BDV-RNAs. In their cerebellums, severe glial fibrosis was observed in the medulla and partly cortex. However, inflammatory changes such as perivascular cell infiltration with lymphocytes and macrophages were not observed in any areas. BDV antigens (p40 and p24) in the neuron and neuropil were not observed with immunohistochemical examinations. In situ PCR methods were revealed the minimal presence of BDV-RNAs in the cytoplasm and nuclei of a few number of neuron.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Red blood cell-substituted NOD-scid mouse model for erythroparasitic protozoan infections., We have previously developed C.B-17 scid mice whose circulating red blood cells (RBCs) had been substituted with RBCs of other animal species. This innovative animal model system greatly facilitated in vivo research for erythroparasitic protozoa that are in nature not infectious to mice. However, the substitution of C.B-17 scid mice with RBCs of some animal species, such as humans and horses, is proved to be very difficult, because of rapid clearance of RBCs transfused in C.B-17 scid mice. In recent years, attempts have been made to develop better SCID mice by changing the genetic background from C.B-17 into the other mice strains, and the NOD-scid mice (the non-obese diabetic mice having the scid mutation) were reported to have significantly improved hematopoietic xeno-transplant acceptability. In the present study, therefore, we examined acceptability of xenogeneic RBCs in NOD/shi-scid in comparison to C.B-17 scid. Clearance tests with fluorescent labeled RBCs of human, dog, horse and cattle showed that life spans of all these RBCs were significantly longer in NOD-scid than in C.B-17 scid mice. In addition, the ability of xeno-RBCs clearance in C.B-17 scid mice was greatly enhanced by some factors, such as optimistic infections, macrophage activating stimuli and aging, whereas such enhancement was poorly observed in NOD-scid mice. These lines of evidence collectively suggest that NOD-scid mice serve as more suitable SCID mice for preparation of the RBC-substitution model than C.B-17 scid. However, NOD-scid mice were less capable of eliminating mouse RBCs upon administration of anti-mouse RBC antibodies, which was effectively used in C.B-17 scid mice to assist rapid RBC-substitution. We were able to demonstrated that NOD-scid mice can easily substituted with human RBCs, and that the human RBC-substituted SCID mice served as an exellent tool for isolation of parasites from a human babesiosis case, which was found for the first time in Japan.

社会貢献活動情報

Social Contribution

  2018 08 01  - 2019 08 31 , Visiting Professor, Trop. Med. Mahidol University, Lecturer, Mahidol University###


教員総覧