College of Agriculture,Food and Environment Sciences, Department of Food Science and Human Wellness
若宮伸隆 ワカミヤ ノブタカ
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Last Update :2020/09/01

Researcher Profile and Settings

Name

WAKAMIYA Nobutaka

Affiliation (Master)

College of Agriculture,Food and Environment Sciences, Department of Food Science and Human Wellness

Education

  1982 04  - 1986 03 , Osaka University, Ph.D course of Medical Science
  1974 04  - 1980 03 , Hirosaki University, School of Medicine

Degree

医学博士, 大阪大学

Committee Memberships

  2016 09  - Today, International Complement Society, Councilor

Academic & Professional Experience

  2018 04  - Today, Rakuno Gakuen University
  1988  - 2000 , Research Institute for Microbial Diseases, Osaka University
  1986  - 1987 , Harvard University

Research Activities

Research Areas

Life sciences, Immunology
Life sciences, Medical biochemistry
Life sciences, Food sciences
Life sciences, Virology
Life sciences, Experimental pathology

Research Interests

complement,

Misc

補体検査プロジェクト報告 検査受諾状況と遺伝子検査, 日高 義彦, 井上 徳光, 中村 道子, 福森 泰雄, 大谷 克城, 若宮 伸隆, 補体, 56, (1) 21 - 22,   2019 07
補体検査プロジェクト報告 補体関連タンパク質検査, 大谷 克城, 井上 徳光, 日高 義彦, 中村 道子, 福森 泰雄, 若宮 伸隆, 補体, 56, (1) 23 - 24,   2019 07
妊娠高血圧症候群における補体マーカー検査と補体関連遺伝子解析の検討, 根木 玲子, 宮田 敏行, 伊田 和史, 小西 妙, 中西 篤史, 吉松 淳, 小亀 浩市, 大谷 克城, 日高 義彦, 若宮 伸隆, 井上 徳光, 補体, 56, (1) 40 - 41,   2019 07
日本補体学会に依頼のあった先天性補体欠損症疑い患者の補体異常, 福森 泰雄, 日高 義彦, 中村 道子, 大谷 克城, 若宮 伸隆, 塚本 浩, 井上 徳光, 補体, 56, (1) 57 - 58,   2019 07
膜型コレクチンCL-P1は、CRPを介して補体経路を活性化する, Roy Nitai, 大谷 克城, 松田 泰幸, 森 健一郎, 黄 仁秀, 井上 徳光, 若宮 伸隆, 日本生化学会大会プログラム・講演要旨集, 89回,   2016 09
新規コレクチンCL-K1は、マウスにおける肺炎球菌感染に対して防御的に働く, 黄 仁秀, 森 健一郎, 大谷 克城, 松田 泰幸, ロイ・ニタイ, 若宮 伸隆, 日本生化学会大会プログラム・講演要旨集, 89回,   2016 09
コレクチンCL-L1の組織局在と分子構造に関する解析, 松田 泰幸, ニタイ・ロイ, 森 健一郎, 黄 仁秀, 大谷 克城, 若宮 伸隆, 日本生化学会大会プログラム・講演要旨集, 86回,   2013 09
コレクチンCL‐P1のリガンド認識ドメインについての解析, 森健一郎, 大谷克城, JANG SeongJae, KIM YounUc, 本村亘, HWANG Insu, 吉田逸朗, 鈴木定彦, 若宮伸隆, 日本糖質学会年会要旨集, 30th,   2011 06 27 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201102221588444941
コレクチンCL‐P1のリガンド認識ドメインの解析, 森健一郎, 大谷克城, JANG SeongJae, KIM YounUck, 本村亘, SUN QiHui, 吉田逸朗, 鈴木定彦, 若宮伸隆, 日本分子生物学会年会講演要旨集, 32nd,   2009 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=201002267174782180
動物血清中のマンナン結合蛋白(MBP)はin vivoにおけるインフルエンザウイルス増殖に関与する, 加瀬哲男, 大谷克城, 江田宗司, 河合高生, 鈴木定彦, 前田章子, 奥野良信, 坂本隆志, 若宮伸隆, 日本免疫学会総会・学術集会記録, 28,   1998 10 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902150300857659
Inhibition of the influenza virus infection by animal serum lectin and its significance., KASE TETSUO, SUZUKI SADAHIKO, EDA SOJI, KAWAI TAKAO, OYA KATSUKI, SAKAMOTO TAKASHI, KURIMURA TAKASHI, WAKAMIYA NOBUTAKA, 日本免疫学会総会・学術集会記録, 26,   1996 10 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902157783529902
リコンビナント動物血清レクチンの作成とそのウイルス感染初期防禦における役割, 若宮伸隆, 坂本隆志, 栗村敬, 江田宗二, 河合高生, 加瀬哲男, 大谷克城, 鈴木定彦, 日本免疫学会総会・学術集会記録, 25,   1995 10 , http://jglobal.jst.go.jp/detail.php?from=API&JGLOBAL_ID=200902142244587144

Research Grants & Projects

Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), The role of CL-K1 in DIC patients, In collectin CL-K1 which Wakamiya discovered, it is supposed as well as conventional collectin that I have a natural immunity function. I performed DIC patient registration and performed sample collection of blood. I measured the inspection item of the DIC mainly on solidification fibrinolysis system. On the other hand, about collectin, I narrowed it down to two of CL-K1 and MBL. In the previous study, CL-K1 took the value that related to in progress of the DIC. However, in the measurement of this Japanese, the meaningful difference was not seen in presence of the DIC.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research, Study of Kawasaki Disease, Kawasaki disease generally develops due to microbe infection and is a disease characterized by intense inflammation of the blood vessel in the whole body. Applicants focused on CL-P1 and went the inspection of the hypothesis to bring about sustained blood vessel inflammation in this study. Firstly, they chose CL-P1 as a candidate to bind CRP and evaluated the activation of the complement to on this occasion that an ascent of sudden CRP was generated when some infection and inflammation happened. They showed that CRP trapped by CL-P1 draws C1q, and activates a classical pathway at this place. Furthermore, it was revealed that this reaction activates the alternative pathway. These results suggest the possibility of exercised new complement activation mechanism through CRP.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Basic study of evaluation system in vascular diseases, The basic study of CL-P1 and CL-K1 has been performed by using recombinant collectins and gene-modified mice. Both established recombinant collectins could produce their antibodies, which were used for the development of ELISA system and evaluation of tissue expressions in those mice and human. The knock-out mice with CL-P1 and CL-K1 have been established. CL-K1 KO mice were analyzed using above antibodies. The prototype ELISA system for the evaluation of its blood concentration was established. The CL-P1 KO and TG mice have been established and their complementation experiments have been performed.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Basic analysis of new collectins in renal tract.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), Study of analysis for vascular damage due to embolism and inflammation, CL-P1 is a new collectin which was found by Ohtani at 2001. It was expressed in vascular endothelial cells in murine tissues and also found in vascular endothelial cell lines. Here we established the antibody against above CL-P1 to investigate their biological functions. We have immunized mice with recombinant CL-P1 protein and made hybridoma for monoclonal antibodies. Several monoclonal antibodies were established and were divided to two or three groups. We tried to make the analysis method to detect CL-P1 using above monoclonal antibodies. We made several prototype assay systems but we could not get a good result because of less CL-P1 volume. Now we began to make a new analysis system using polyclonal antibodies.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B), The study on functions on the membrane type collectm CL-P1 in vascular cells., Collectins are a family of C type lectins which have collagen-like sequences and carbohydrate recognition domains (CRD). The scavenger receptors type A and MARCO are classical type scavenger receptors which have internal collagen-like domains. Here, we found a new scavenger receptor which is a membrane type collectin from placenta (collectin placenta 1=CL-P1) that has a typical collectin collagen-like domain and a CRD. To elucidate the functions in CL-P1 in animal body is our purpose. In 3 years, we finished seven projects below, 1.Cloning of CL-P1 cDNA in human, mouse, rat, zebra fish, xenopus. 2.Establishment of CL-P1 permanent expression cells. 3.Production of soluble recombinant CL-P1 and mutant one having deletion or point mutation. 4.Discovery of binding protein against CL-P1. 5.Analyses oxidized LDLs after having different oxidation treatment. 6.Study on the promoter activity in CL-P1 5'-franking region. 7.Study on the kinetics of CL-P1 after several physiological treatments.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), The relationship between the stress and serum mannan-binding lectin., Mannan-binding lectin (MBL) is a C-type serum lectin which is believed to play an important role in innate immunity.It is one of the collectin family which are characterized by having a collagen-like sequence and a carbohydrate recognition domain.Collectin can bind to sugar determinants of several microorganisms, neutralize them and inhibit infection by the of complement activation through the lectin pathway and opsonization by collectin receptors.First we tried to isolate collectin genes in several animal and establish the production system of collectin in E.coli and CHO cells.Next to reveal the role of collectin under the stress condition, we investigated the serum concentration from persons who were given with/without stress. (1) We isolated and characterized the cDNA encoding bovine and rabbit MBP (Kawai et al., Gene. 1997, Glycobiology. 1998). (2) We established the high and effective production system of bovine conglutinin and human SP-D and MBP (Eda et al, Biochem. J.1997, Biosci. Biotech. Bioch., 1998., Suzuki, et al., Biochem. Biophys. Res. Commun., 1997, Ohtani et al., J.Immunol. Methods, 1999). (3) We measured the serum concentration and gene allele of MBP in persons from different groups.We found only the gene mutation of codon 54 in all Japanese population (over 700 samples).Fulminant Hepatitis patient (death group) was seen in low MBP level, in comparison with the patient (survived group).
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for General Scientific Research (C), PURIFICATION AND CHARACTERIZATION OF INFLUENZA A VIRUS INHIBITORS IN ANIMAL SERA, Normal horse and guinea pig sera contain a2-macroglobulin which inhibits the infectivity and hemagglutinating activity of influenza A viruses of the H2 and H3 subtypes. On the other hand, normal bovine serum contains a component termed beta inhibitor that inhibits the infectivity and hemagglutinating activity on influenza A viruses of the H1 and H3 subtypes. To investigate the nature of the beta inhibitor of influenza A virus, we purified the conglutinin and examined its characteristics. The conglutinin , purified from bovine serum, had neutralizing-activity as well as HI activity on influenza A viruses of the H1 and H3 subtypes. The HI activity of conglutinin was heat stable, Ca^<++>-dependent, and resistant to both neuraminidase and periodate treatments. The HI activity of purified conglutinin was blocked by N-acetylglucosamine but not by D- mannose. The conglutinin was bound to hemagglutinin which had high mannose and complex sugar chains and its binding was inhibited by N- acetylglucosamine and dependent on devalent cations. These data indicate that the beta-like inhibitor activity of bovine serum is mainly dependent on conglutinin which inhibits hemagglutination and neutralizes the virus infectivity by its binding to a carbohydrate site at the HA. Nextly, we tried to clone the cDNA coding for bovine conglutinin. cDNA clones encoding the bovine conglutinin were isolated from a bovine liver cDNA library using a specific probe obtained from the PCR product. These cDNAs carry an insert of 1113 bp coding for a protein of 371 amino acid residues with a signal peptide of 20 residues. Southern blot analysis of total bovine genomic DNA indicated that there is only one copy of the gene encoding bovine conglutinin. Northern blot analysis of bovine tissues showed that conglutinin mRNA of about 1.5 kb is expressed in the liver and also slightly in the lung.
Japan Society for the Promotion of Science, Grants-in-Aid for Scientific Research Grant-in-Aid for General Scientific Research (A), Inhalation anesthetics and oncogene, Although almost cancer patients are undergone with general anesthesia, a few researchers and anesthesiologists are interest in the interaction between cancer cells and anesthesia. So we have been doing researches of the influence of anesthesia on cancer cells in order to try to establish the best anesthesia for cancer patients. We put on a focus on "oncogene". We guessed that anesthesia agents might influence oncogene. We studied as follows; (1)halothane and the expression of c-myc protein in human leukemia cells in vitro, (2)halothane and the amplification of N-myc in neuroblastoma in vitro. Results were as follows; (1)the expression of c-myc protein did not change during/after halothane exposure, (2)the amplification of N-myc did not also change during/after halothane exposure. As far as our results, anesthesia did not influence oncogene. There are many other oncogenes. Some of other oncogene might change during/after anesthesia. Furthermore, we did other researches. We tried to evaluate the change of antigens in cancer cells during/after anesthesia in vitro. We studied as follows; (1)halothane and the expression of HLA-DR antigen in melanoma cells, (2)halothane/sevoflurane and the expression of intercellular adhesion molecule-1 (ICAM-1) in melanoma cells. The results were as follows; (1)the expression of HLA-DR in melanoma cells increased after halothane exposure, (2)the expression of ICAM-1 in melanoma cells decreased during halothane/sevoflurane exposure. These results suggested that anesthetic agents influence on antigen expression in cancer cells, and that the change might decrease the recognition of immune surveillance. Anesthetic agents might deteriorate cancer patients. Anesthesiologists and surgeons should be aware that anesthesia might deteriorate cancer patients, and had better continue to do research on the relation between cancer and anesthesia.


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